Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0016296

Physical Location & Seq

Genomic Coordinates Reverse (-)

4012128 .. 4015459

Gene Structure 3332 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1050200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 846 bp

ATGTCAGGAGGAAGACCAATGGCAGAGAAGGAGAGTACAATGAGTGGAATATTACCAAACTATCATCATCCTCATCATCATCATTTGGTTCTGATCCATGGTATCGGGCATGGAGCTTGGTGCTGGTACAAGATCCGGTGTCTAATGGAAGCTTCAGGCTACAAAGTCACATGTCTTGACCTCAAAGGTGCTGGTGTTGATCAATCTGACCCTAATACCGTCCTCACTTTCCAAGACTACAATCAGCCTCTCACTGACTTCTTGTCCAGTTTGCCAGGAAATGAAAGGGTAATACTCGTGGGACATAGTGCAGGAGGTATGAGCCTGACTGATGCCATACACAGGTTTGCTAGCAAAATACATATGGCGATATATGTTGCAGCAACCATGTTGGAGCATGGATTTTCCACACATCAAGATATCATAGATGGAGAACCTGACTTATCGGCATTTGGAAACGTAAACAAGTTCATATATGGGTTGGGAGTTGACCAGCCTCCAACTAGTGTCATAATAAAGGAAAATTTTCATCACCAAATCTTGTACCAGATGAGCCCTATAGAGGACGCAACTTTAGCTTCAATGCTGCTGAGGCCAGGACCTGTTATGGCGTTGAAAGGCGCTCGCTTTCAAGGAAACAAGGAAGCTGCTGATCGTGTGCCAAGAGTGTACATCAAGACCATGCATGACCATGTTCTAAAGCTTGAGCAGCAAGATGCCATGATCAAGAGATGGCCACCATCCCAGGTCTTTGTTCTCCAAAGCGATCATAGCCCGTTTTTCTCCACCCCCATTTTGCTCTTTGGTTTGTTGGTTAAAGCATTGGCTTCCATCAAATGTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006082 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009683 GO:0009850 GO:0009987 GO:0010817 GO:0016787 GO:0016788 GO:0019752 GO:0032501 GO:0032502 GO:0032787 GO:0033473 GO:0034641 GO:0034754 GO:0042430 GO:0042445 GO:0043436 GO:0044237 GO:0044281 GO:0046483 GO:0048367 GO:0048731 GO:0048856 GO:0052689 GO:0065007 GO:0065008 GO:0071704 GO:0080030 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6

Protein Analysis

282

Amino Acids

31.13

Weight (kDa)

6.8

Isoelectric Point (pI)

43.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase_4	PF12146	25 - 132	2e-10	Serine aminopeptidase, S33
Abhydrolase_1	PF00561	28 - 133	1.4e-12	alpha/beta hydrolase fold
Abhydrolase_6	PF12697	29 - 264	8.7e-19	Alpha/beta hydrolase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016296)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g40710 FvH4_3g40710
malus_domestica	MD03G1050200.v1.1 MD03G1050400.v1.1
prunus_persica	Prupe.6G040000_v2.0.a1 Prupe.6G040000_v2.0.a1 Prupe.6G040000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0073501
rosa_multiflora	Rmu_ssc0000226.1_g000015
rosa_rugosa	Rorug05G0423900 Rorug05G0424000
rosa_samantha	Rh5AG481300 Rh5BG502000 Rh5CG525200
rosa_wichuraiana	Rw5G044710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AclWI	GGATC	2 cut(s)	88, 127
AcoI	YGGCCR	1 cut(s)	734
AcsI	RAATTY	1 cut(s)	523
AcuI	CTGAAG	1 cut(s)	138
AfaI	GTAC	4 cut(s)	37, 128, 545, 671
AfiI	CCNNNNNNNGG	2 cut(s)	342, 562
AflIII	ACRYGT	1 cut(s)	170
AgsI	TTSAA	3 cut(s)	582, 616, 632
AhdI	GACNNNNNGTC	1 cut(s)	262
AhlI	ACTAGT	1 cut(s)	503
AjnI	CCWGG	3 cut(s)	274, 595, 744
AluBI	AGCT	5 cut(s)	116, 152, 578, 647, 703
AluI	AGCT	5 cut(s)	116, 152, 578, 647, 703
AlwI	GGATC	2 cut(s)	88, 127
AlwNI	CAGNNNCTG	1 cut(s)	602
AoxI	GGCC	2 cut(s)	593, 734
ApeKI	GCWGC	4 cut(s)	380, 586, 647, 709
ApoI	RAATTY	1 cut(s)	523
Asp700I	GAANNNNTTC	1 cut(s)	525
AspLEI	GCGC	1 cut(s)	623
AspS9I	GGNCC	1 cut(s)	599
AsuHPI	GGTGA	1 cut(s)	524
AsuNHI	GCTAGC	1 cut(s)	350
AvaII	GGWCC	1 cut(s)	599
BalI	TGGCCA	1 cut(s)	736
BanII	GRGCYC	1 cut(s)	557
BauI	CACGAG	1 cut(s)	296
BbsI	GAAGAC	1 cut(s)	19
BbvCI	CCTCAGC	1 cut(s)	590
BbvI	GCAGC	4 cut(s)	392, 573, 634, 721
BccI	CCATC	4 cut(s)	422, 726, 748, 839
BciT130I	CCWGG	3 cut(s)	276, 597, 746
BclI	TGATCA	2 cut(s)	199, 723
BcuI	ACTAGT	1 cut(s)	503
BfaI	CTAG	2 cut(s)	351, 504
BfmI	CTRYAG	1 cut(s)	558
BfoI	RGCGCY	1 cut(s)	624
BisI	GCNGC	4 cut(s)	381, 587, 648, 710
BlsI	GCNGC	4 cut(s)	382, 588, 649, 711
Bme1390I	CCNGG	3 cut(s)	276, 597, 746
Bme18I	GGWCC	1 cut(s)	599
BmeRI	GACNNNNNGTC	1 cut(s)	262
BmgT120I	GGNCC	1 cut(s)	599
BmrFI	CCNGG	3 cut(s)	276, 597, 746
BmsI	GCATC	2 cut(s)	322, 706
BmtI	GCTAGC	1 cut(s)	354
BpiI	GAAGAC	1 cut(s)	19
Bpu10I	CCTNAGC	1 cut(s)	590
BpuEI	CTTGAG	1 cut(s)	725
BsaJI	CCNNGG	2 cut(s)	97, 744
BsaWI	WCCGGW	1 cut(s)	135
Bsc4I	CCNNNNNNNGG	2 cut(s)	342, 562
Bse1I	ACTGG	1 cut(s)	267
BseBI	CCWGG	3 cut(s)	276, 597, 746
BseDI	CCNNGG	2 cut(s)	97, 744
BseGI	GGATG	2 cut(s)	67, 740
BseLI	CCNNNNNNNGG	2 cut(s)	342, 562
BseMII	CTCAG	1 cut(s)	581
BseNI	ACTGG	1 cut(s)	267
BseXI	GCAGC	4 cut(s)	392, 573, 634, 721
BsgI	GTGCAG	1 cut(s)	330
BshFI	GGCC	2 cut(s)	595, 736
BsiSI	CCGG	1 cut(s)	136
BslFI	GGGAC	1 cut(s)	315
BslI	CCNNNNNNNGG	2 cut(s)	342, 562
BsmFI	GGGAC	1 cut(s)	315
BsnI	GGCC	2 cut(s)	595, 736
Bsp1286I	GDGCHC	1 cut(s)	557
Bsp1407I	TGTACA	1 cut(s)	669
Bsp143I	GATC	6 cut(s)	93, 132, 199, 652, 723, 766
Bsp19I	CCATGG	1 cut(s)	97
BspANI	GGCC	2 cut(s)	595, 736
BspCNI	CTCAG	1 cut(s)	582
BspOI	GCTAGC	1 cut(s)	354
BspPI	GGATC	2 cut(s)	88, 127
BsrGI	TGTACA	1 cut(s)	669
BsrI	ACTGG	1 cut(s)	267
BssECI	CCNNGG	2 cut(s)	97, 744
BssMI	GATC	6 cut(s)	93, 132, 199, 652, 723, 766
BssSI	CACGAG	1 cut(s)	296
BssT1I	CCWWGG	1 cut(s)	97
Bst2BI	CACGAG	1 cut(s)	296
Bst2UI	CCWGG	3 cut(s)	276, 597, 746
Bst4CI	ACNGT	1 cut(s)	220
BstAUI	TGTACA	1 cut(s)	669
BstC8I	GCNNGC	2 cut(s)	352, 625
BstDEI	CTNAG	1 cut(s)	590
BstDSI	CCRYGG	1 cut(s)	97
BstF5I	GGATG	2 cut(s)	67, 740
BstH2I	RGCGCY	1 cut(s)	624
BstHHI	GCGC	1 cut(s)	623
BstKTI	GATC	6 cut(s)	96, 135, 202, 655, 726, 769
BstMBI	GATC	6 cut(s)	93, 132, 199, 652, 723, 766
BstMWI	GCNNNNNNNGC	4 cut(s)	575, 592, 709, 771
BstNI	CCWGG	3 cut(s)	276, 597, 746
BstNSI	RCATGY	1 cut(s)	174
BstSCI	CCNGG	3 cut(s)	274, 595, 744
BstSFI	CTRYAG	1 cut(s)	558
BstV1I	GCAGC	4 cut(s)	392, 573, 634, 721
BstV2I	GAAGAC	1 cut(s)	19
BstX2I	RGATCY	1 cut(s)	132
BstYI	RGATCY	1 cut(s)	132
BsuRI	GGCC	2 cut(s)	595, 736
BtgI	CCRYGG	1 cut(s)	97
BtsCI	GGATG	2 cut(s)	67, 740
BtsIMutI	CAGTG	1 cut(s)	252
Cac8I	GCNNGC	2 cut(s)	352, 625
CaiI	CAGNNNCTG	1 cut(s)	602
CfoI	GCGC	1 cut(s)	623
Cfr13I	GGNCC	1 cut(s)	599
CseI	GACGC	1 cut(s)	575
Csp6I	GTAC	4 cut(s)	36, 127, 544, 670
CviAII	CATG	9 cut(s)	98, 110, 171, 388, 398, 682, 686, 692, 721
CviQI	GTAC	4 cut(s)	36, 127, 544, 670
DdeI	CTNAG	1 cut(s)	590
DpnI	GATC	6 cut(s)	95, 134, 201, 654, 725, 768
DpnII	GATC	6 cut(s)	93, 132, 199, 652, 723, 766
DriI	GACNNNNNGTC	1 cut(s)	262
EaeI	YGGCCR	1 cut(s)	734
Eam1105I	GACNNNNNGTC	1 cut(s)	262
Eco130I	CCWWGG	1 cut(s)	97
Eco24I	GRGCYC	1 cut(s)	557
Eco32I	GATATC	1 cut(s)	421
Eco47I	GGWCC	1 cut(s)	599
Eco57I	CTGAAG	1 cut(s)	138
EcoO109I	RGGNCCY	1 cut(s)	599
EcoRII	CCWGG	3 cut(s)	274, 595, 744
EcoRV	GATATC	1 cut(s)	421
EcoT14I	CCWWGG	1 cut(s)	97
EcoT22I	ATGCAT	1 cut(s)	687
EcoT38I	GRGCYC	1 cut(s)	557
ErhI	CCWWGG	1 cut(s)	97
FaeI	CATG	9 cut(s)	101, 113, 174, 391, 401, 685, 689, 695, 724
FaqI	GGGAC	1 cut(s)	315
FatI	CATG	9 cut(s)	97, 109, 170, 387, 397, 681, 685, 691, 720
FauNDI	CATATG	1 cut(s)	363
FbaI	TGATCA	2 cut(s)	199, 723
Fnu4HI	GCNGC	4 cut(s)	381, 587, 648, 710
FokI	GGATG	2 cut(s)	54, 727
FriOI	GRGCYC	1 cut(s)	557
Fsp4HI	GCNGC	4 cut(s)	381, 587, 648, 710
FspBI	CTAG	2 cut(s)	351, 504
GlaI	GCGC	1 cut(s)	622
GluI	GCNGC	4 cut(s)	381, 587, 648, 710
HaeII	RGCGCY	1 cut(s)	624
HaeIII	GGCC	2 cut(s)	595, 736
HapII	CCGG	1 cut(s)	136
HgaI	GACGC	1 cut(s)	575
HhaI	GCGC	1 cut(s)	623
Hin1II	CATG	9 cut(s)	101, 113, 174, 391, 401, 685, 689, 695, 724
Hin6I	GCGC	1 cut(s)	621
HinP1I	GCGC	1 cut(s)	621
HincII	GTYRAC	1 cut(s)	490
HindII	GTYRAC	1 cut(s)	490
HindIII	AAGCTT	2 cut(s)	150, 701
HpaII	CCGG	1 cut(s)	136
HphI	GGTGA	1 cut(s)	524
Hpy166II	GTNNAC	3 cut(s)	463, 490, 670
Hpy188I	TCNGA	2 cut(s)	93, 208
Hpy188III	TCNNGA	5 cut(s)	6, 176, 416, 676, 727
Hpy8I	GTNNAC	3 cut(s)	463, 490, 670
HpyAV	CCTTC	1 cut(s)	22
HpyCH4III	ACNGT	1 cut(s)	220
HpyCH4IV	ACGT	1 cut(s)	459
HpyCH4V	TGCA	3 cut(s)	311, 380, 685
HpyF10VI	GCNNNNNNNGC	4 cut(s)	575, 592, 709, 771
HpyF3I	CTNAG	1 cut(s)	590
HpySE526I	ACGT	1 cut(s)	459
Hsp92II	CATG	9 cut(s)	101, 113, 174, 391, 401, 685, 689, 695, 724
HspAI	GCGC	1 cut(s)	621
Ksp22I	TGATCA	2 cut(s)	199, 723
Kzo9I	GATC	6 cut(s)	93, 132, 199, 652, 723, 766
LmnI	GCTCC	2 cut(s)	113, 394
Lsp1109I	GCAGC	4 cut(s)	392, 573, 634, 721
LweI	GCATC	2 cut(s)	322, 706
MaeI	CTAG	2 cut(s)	351, 504
MaeII	ACGT	1 cut(s)	459
MaeIII	GTNAC	1 cut(s)	166
MalI	GATC	6 cut(s)	95, 134, 201, 654, 725, 768
MboI	GATC	6 cut(s)	93, 132, 199, 652, 723, 766
MboII	GAAGA	1 cut(s)	24
MflI	RGATCY	1 cut(s)	132
MhlI	GDGCHC	1 cut(s)	557
MlsI	TGGCCA	1 cut(s)	736
MluCI	AATT	1 cut(s)	523
MluNI	TGGCCA	1 cut(s)	736
MmeI	TCCRAC	2 cut(s)	372, 524
MnlI	CCTC	8 cut(s)	81, 191, 233, 258, 308, 507, 556, 585
Mox20I	TGGCCA	1 cut(s)	736
Mph1103I	ATGCAT	1 cut(s)	687
MroXI	GAANNNNTTC	1 cut(s)	525
MscI	TGGCCA	1 cut(s)	736
MseI	TTAA	2 cut(s)	816, 844
MslI	CAYNNNNRTG	1 cut(s)	690
Msp20I	TGGCCA	1 cut(s)	736
MspI	CCGG	1 cut(s)	136
MspR9I	CCNGG	3 cut(s)	276, 597, 746
MvaI	CCWGG	3 cut(s)	276, 597, 746
MwoI	GCNNNNNNNGC	4 cut(s)	575, 592, 709, 771
NcoI	CCATGG	1 cut(s)	97
NdeI	CATATG	1 cut(s)	363
NdeII	GATC	6 cut(s)	93, 132, 199, 652, 723, 766
NheI	GCTAGC	1 cut(s)	350
NlaIII	CATG	9 cut(s)	101, 113, 174, 391, 401, 685, 689, 695, 724
NmuCI	GTSAC	1 cut(s)	166
NsiI	ATGCAT	1 cut(s)	687
NspI	RCATGY	1 cut(s)	174
PciI	ACATGT	1 cut(s)	170
PdmI	GAANNNNTTC	1 cut(s)	525
PkrI	GCNGC	4 cut(s)	382, 588, 649, 711
PpuMI	RGGWCCY	1 cut(s)	599
PscI	ACATGT	1 cut(s)	170
Psp5II	RGGWCCY	1 cut(s)	599
Psp6I	CCWGG	3 cut(s)	274, 595, 744
PspGI	CCWGG	3 cut(s)	274, 595, 744
PspPI	GGNCC	1 cut(s)	599
PspPPI	RGGWCCY	1 cut(s)	599
PstNI	CAGNNNCTG	1 cut(s)	602
PsuI	RGATCY	1 cut(s)	132
RsaI	GTAC	4 cut(s)	37, 128, 545, 671
RsaNI	GTAC	4 cut(s)	36, 127, 544, 670
RseI	CAYNNNNRTG	1 cut(s)	690
SaqAI	TTAA	2 cut(s)	816, 844
SatI	GCNGC	4 cut(s)	381, 587, 648, 710
Sau3AI	GATC	6 cut(s)	93, 132, 199, 652, 723, 766
Sau96I	GGNCC	1 cut(s)	599
ScrFI	CCNGG	3 cut(s)	276, 597, 746
SduI	GDGCHC	1 cut(s)	557
SfaNI	GCATC	2 cut(s)	322, 706
SfcI	CTRYAG	1 cut(s)	558
SinI	GGWCC	1 cut(s)	599
SmiMI	CAYNNNNRTG	1 cut(s)	690
SmlI	CTYRAG	1 cut(s)	704
SmoI	CTYRAG	1 cut(s)	704
SpeI	ACTAGT	1 cut(s)	503
Sse9I	AATT	1 cut(s)	523
SspI	AATATT	1 cut(s)	51
SspMI	CTAG	2 cut(s)	351, 504
StyD4I	CCNGG	3 cut(s)	274, 595, 744
StyI	CCWWGG	1 cut(s)	97
TaaI	ACNGT	1 cut(s)	220
TaiI	ACGT	1 cut(s)	462
TasI	AATT	1 cut(s)	523
TatI	WGTACW	2 cut(s)	35, 669
Tru1I	TTAA	2 cut(s)	816, 844
Tru9I	TTAA	2 cut(s)	816, 844
TscAI	CASTG	1 cut(s)	259
TseFI	GTSAC	1 cut(s)	166
TseI	GCWGC	4 cut(s)	380, 586, 647, 709
Tsp45I	GTSAC	1 cut(s)	166
TspDTI	ATGAA	3 cut(s)	297, 460, 518
TspRI	CASTG	1 cut(s)	259
VpaK11BI	GGWCC	1 cut(s)	599
XapI	RAATTY	1 cut(s)	523
XceI	RCATGY	1 cut(s)	174
XmnI	GAANNNNTTC	1 cut(s)	525
XspI	CTAG	2 cut(s)	351, 504
Zsp2I	ATGCAT	1 cut(s)	687

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.