Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0016296

Physical Location & Seq

Genomic Coordinates Reverse (-)

4019296 .. 4020134

Gene Structure 839 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1050400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 591 bp

ATGTCAGGAGGAAGACCAATGGTAGAGAAGGAGAGTACAATGAGTGGAATATTACCAAACTATCATCATCCTCATCATCATCATTTGGTTCTGATCCATGGTATAGGGCATGGAGCTTGGTGCTGGTACAAGATCCGGTGTCTAATGGAAGCTTCAGGCTACAAAGTCACATGTCTTGACCTCAAAGGTGCTGGTGTTGATCAATCTGATCCTAATACCGTCCTCACTTTCCAAGACTATAACCAGCCTCTCACTGACTTCTTGTCCAGTTTGCCAGGAAATGAAAGGGTAATACTTGTGGGACATAGTGCAGGAGGTATGAGCCTGACTGATGCCATACACAGGTTTGCTAGCAAAATACATATGGCGATATATGTTGCAGCAACCATGTTGGAGCATGGATTTTCCACCCATCAAGATATCATAGATGTAAGAATATTAATTTCTTCTATTCCATTTTTCTTCTTATTAAAATCCTCTTCATTTTTAAGAAAAGTTCTTCGGAGTTCAAGTGCTATACTATGTAATTTTCTTGTCTTTTCCCTTTATTTACACGGAAAAATAGGCAAACTTACATATTATAATTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006082 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009683 GO:0009850 GO:0009987 GO:0010817 GO:0016787 GO:0016788 GO:0019752 GO:0032501 GO:0032502 GO:0032787 GO:0033473 GO:0034641 GO:0034754 GO:0042430 GO:0042445 GO:0043436 GO:0044237 GO:0044281 GO:0046483 GO:0048367 GO:0048731 GO:0048856 GO:0052689 GO:0065007 GO:0065008 GO:0071704 GO:0080030 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6

Protein Analysis

197

Amino Acids

21.89

Weight (kDa)

8.67

Isoelectric Point (pI)

35.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase_4	PF12146	25 - 131	1.2e-10	Serine aminopeptidase, S33
Abhydrolase_1	PF00561	28 - 132	7.3e-13	alpha/beta hydrolase fold
Abhydrolase_6	PF12697	29 - 139	8.2e-15	Alpha/beta hydrolase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0016296)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g40710 FvH4_3g40710
malus_domestica	MD03G1050200.v1.1 MD03G1050400.v1.1
prunus_persica	Prupe.6G040000_v2.0.a1 Prupe.6G040000_v2.0.a1 Prupe.6G040000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0073501
rosa_multiflora	Rmu_ssc0000226.1_g000015
rosa_rugosa	Rorug05G0423900 Rorug05G0424000
rosa_samantha	Rh5AG481300 Rh5BG502000 Rh5CG525200
rosa_wichuraiana	Rw5G044710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	582
AclWI	GGATC	3 cut(s)	88, 127, 203
AcuI	CTGAAG	1 cut(s)	138
AfaI	GTAC	2 cut(s)	37, 128
AfiI	CCNNNNNNNGG	1 cut(s)	342
AflIII	ACRYGT	1 cut(s)	170
AgsI	TTSAA	1 cut(s)	510
AhdI	GACNNNNNGTC	1 cut(s)	262
AjnI	CCWGG	1 cut(s)	274
AluBI	AGCT	2 cut(s)	116, 152
AluI	AGCT	2 cut(s)	116, 152
AlwI	GGATC	3 cut(s)	88, 127, 203
ApeKI	GCWGC	1 cut(s)	380
AseI	ATTAAT	1 cut(s)	440
AsuNHI	GCTAGC	1 cut(s)	350
BbsI	GAAGAC	1 cut(s)	19
BbvI	GCAGC	1 cut(s)	392
BccI	CCATC	1 cut(s)	420
BciT130I	CCWGG	1 cut(s)	276
BclI	TGATCA	1 cut(s)	199
BfaI	CTAG	1 cut(s)	351
BisI	GCNGC	1 cut(s)	381
BlsI	GCNGC	1 cut(s)	382
Bme1390I	CCNGG	1 cut(s)	276
BmeRI	GACNNNNNGTC	1 cut(s)	262
BmrFI	CCNGG	1 cut(s)	276
BmsI	GCATC	1 cut(s)	322
BmtI	GCTAGC	1 cut(s)	354
BpiI	GAAGAC	1 cut(s)	19
BsaJI	CCNNGG	1 cut(s)	97
BsaWI	WCCGGW	1 cut(s)	135
BsaXI	ACNNNNNCTCC	2 cut(s)	496, 526
Bsc4I	CCNNNNNNNGG	1 cut(s)	342
Bse1I	ACTGG	1 cut(s)	267
BseBI	CCWGG	1 cut(s)	276
BseDI	CCNNGG	1 cut(s)	97
BseGI	GGATG	1 cut(s)	67
BseLI	CCNNNNNNNGG	1 cut(s)	342
BseNI	ACTGG	1 cut(s)	267
BseXI	GCAGC	1 cut(s)	392
BsgI	GTGCAG	1 cut(s)	330
BsiSI	CCGG	1 cut(s)	136
BslFI	GGGAC	1 cut(s)	315
BslI	CCNNNNNNNGG	1 cut(s)	342
BsmFI	GGGAC	1 cut(s)	315
Bsp143I	GATC	4 cut(s)	93, 132, 199, 208
Bsp19I	CCATGG	1 cut(s)	97
BspOI	GCTAGC	1 cut(s)	354
BspPI	GGATC	3 cut(s)	88, 127, 203
BsrI	ACTGG	1 cut(s)	267
BssECI	CCNNGG	1 cut(s)	97
BssMI	GATC	4 cut(s)	93, 132, 199, 208
BssT1I	CCWWGG	1 cut(s)	97
Bst2UI	CCWGG	1 cut(s)	276
Bst4CI	ACNGT	1 cut(s)	220
Bst6I	CTCTTC	1 cut(s)	484
BstC8I	GCNNGC	1 cut(s)	352
BstDSI	CCRYGG	1 cut(s)	97
BstF5I	GGATG	1 cut(s)	67
BstKTI	GATC	4 cut(s)	96, 135, 202, 211
BstMBI	GATC	4 cut(s)	93, 132, 199, 208
BstNI	CCWGG	1 cut(s)	276
BstNSI	RCATGY	1 cut(s)	174
BstSCI	CCNGG	1 cut(s)	274
BstV1I	GCAGC	1 cut(s)	392
BstV2I	GAAGAC	1 cut(s)	19
BstX2I	RGATCY	1 cut(s)	132
BstYI	RGATCY	1 cut(s)	132
BtgI	CCRYGG	1 cut(s)	97
BtsCI	GGATG	1 cut(s)	67
BtsIMutI	CAGTG	1 cut(s)	252
Cac8I	GCNNGC	1 cut(s)	352
Csp6I	GTAC	2 cut(s)	36, 127
CviAII	CATG	5 cut(s)	98, 110, 171, 388, 398
CviJI	RGCY	5 cut(s)	116, 152, 159, 247, 324
CviKI_1	RGCY	5 cut(s)	116, 152, 159, 247, 324
CviQI	GTAC	2 cut(s)	36, 127
DpnI	GATC	4 cut(s)	95, 134, 201, 210
DpnII	GATC	4 cut(s)	93, 132, 199, 208
DriI	GACNNNNNGTC	1 cut(s)	262
Eam1104I	CTCTTC	1 cut(s)	484
Eam1105I	GACNNNNNGTC	1 cut(s)	262
EarI	CTCTTC	1 cut(s)	484
Eco130I	CCWWGG	1 cut(s)	97
Eco32I	GATATC	1 cut(s)	421
Eco57I	CTGAAG	1 cut(s)	138
EcoRII	CCWGG	1 cut(s)	274
EcoRV	GATATC	1 cut(s)	421
EcoT14I	CCWWGG	1 cut(s)	97
ErhI	CCWWGG	1 cut(s)	97
FaeI	CATG	5 cut(s)	101, 113, 174, 391, 401
FaqI	GGGAC	1 cut(s)	315
FatI	CATG	5 cut(s)	97, 109, 170, 387, 397
FauNDI	CATATG	1 cut(s)	363
FbaI	TGATCA	1 cut(s)	199
Fnu4HI	GCNGC	1 cut(s)	381
FokI	GGATG	1 cut(s)	54
Fsp4HI	GCNGC	1 cut(s)	381
FspBI	CTAG	1 cut(s)	351
GluI	GCNGC	1 cut(s)	381
HapII	CCGG	1 cut(s)	136
Hin1II	CATG	5 cut(s)	101, 113, 174, 391, 401
HindIII	AAGCTT	1 cut(s)	150
HpaII	CCGG	1 cut(s)	136
Hpy188I	TCNGA	3 cut(s)	93, 208, 504
Hpy188III	TCNNGA	3 cut(s)	6, 176, 416
HpyAV	CCTTC	1 cut(s)	22
HpyCH4III	ACNGT	1 cut(s)	220
HpyCH4V	TGCA	2 cut(s)	311, 380
Hsp92II	CATG	5 cut(s)	101, 113, 174, 391, 401
Ksp22I	TGATCA	1 cut(s)	199
Kzo9I	GATC	4 cut(s)	93, 132, 199, 208
LmnI	GCTCC	2 cut(s)	113, 394
Lsp1109I	GCAGC	1 cut(s)	392
LweI	GCATC	1 cut(s)	322
MaeI	CTAG	1 cut(s)	351
MaeIII	GTNAC	1 cut(s)	166
MalI	GATC	4 cut(s)	95, 134, 201, 210
MboI	GATC	4 cut(s)	93, 132, 199, 208
MboII	GAAGA	5 cut(s)	24, 438, 454, 471, 491
MflI	RGATCY	1 cut(s)	132
MluCI	AATT	3 cut(s)	441, 526, 583
MmeI	TCCRAC	1 cut(s)	372
MnlI	CCTC	6 cut(s)	81, 191, 233, 258, 308, 487
MseI	TTAA	3 cut(s)	440, 470, 488
MspI	CCGG	1 cut(s)	136
MspR9I	CCNGG	1 cut(s)	276
MvaI	CCWGG	1 cut(s)	276
NcoI	CCATGG	1 cut(s)	97
NdeI	CATATG	1 cut(s)	363
NdeII	GATC	4 cut(s)	93, 132, 199, 208
NheI	GCTAGC	1 cut(s)	350
NlaIII	CATG	5 cut(s)	101, 113, 174, 391, 401
NmuCI	GTSAC	1 cut(s)	166
NspI	RCATGY	1 cut(s)	174
PciI	ACATGT	1 cut(s)	170
PkrI	GCNGC	1 cut(s)	382
PscI	ACATGT	1 cut(s)	170
PshBI	ATTAAT	1 cut(s)	440
PsiI	TTATAA	1 cut(s)	582
Psp6I	CCWGG	1 cut(s)	274
PspGI	CCWGG	1 cut(s)	274
PsuI	RGATCY	1 cut(s)	132
RsaI	GTAC	2 cut(s)	37, 128
RsaNI	GTAC	2 cut(s)	36, 127
SaqAI	TTAA	3 cut(s)	440, 470, 488
SatI	GCNGC	1 cut(s)	381
Sau3AI	GATC	4 cut(s)	93, 132, 199, 208
ScrFI	CCNGG	1 cut(s)	276
SetI	ASST	6 cut(s)	118, 154, 183, 190, 319, 347
SfaNI	GCATC	1 cut(s)	322
Sse9I	AATT	3 cut(s)	441, 526, 583
SspI	AATATT	2 cut(s)	51, 438
SspMI	CTAG	1 cut(s)	351
StyD4I	CCNGG	1 cut(s)	274
StyI	CCWWGG	1 cut(s)	97
TaaI	ACNGT	1 cut(s)	220
TasI	AATT	3 cut(s)	441, 526, 583
TatI	WGTACW	1 cut(s)	35
Tru1I	TTAA	3 cut(s)	440, 470, 488
Tru9I	TTAA	3 cut(s)	440, 470, 488
TscAI	CASTG	1 cut(s)	259
TseFI	GTSAC	1 cut(s)	166
TseI	GCWGC	1 cut(s)	380
Tsp45I	GTSAC	1 cut(s)	166
TspDTI	ATGAA	2 cut(s)	297, 471
TspGWI	ACGGA	1 cut(s)	570
TspRI	CASTG	1 cut(s)	259
VspI	ATTAAT	1 cut(s)	440
XceI	RCATGY	1 cut(s)	174
XspI	CTAG	1 cut(s)	351

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.