Rorug05G0424000

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0016296
Physical Location & Seq
Forward (+)
58929902 .. 58930633
732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0424000.1

Sequence Viewer

Length: 732 bp
ATGTATAATGTTTTGGCCATATTTCTTCTGGCTTTTGTTTTTCTGGCTGGAGCATGGTTGGGATTCTGGACAGTTCGTAAAATGGCCTTGAACGAAGATGGATCAATTGATTCTAGCACATCTCTGTTTGTTTTGTGGACCATTCGCTTCGTAGCTGTTATTTTTCTTTCACAGAGTTCCAGCGATCAACTACTGGCAGCAGGAGCTATAATGGTACCTTTAGTCCCTTTTATAGTGAGGAAAATTGCCATTATACTCGGAACTTTAGTCCCTTTAATCAGGAAAATCACTATATCCTTTGGACCTATAGTCGTTTTCATAGCGAGGACATTCACTATAGTATGTGGATGCATAATCTCTTCAGCAAGGGAAATCTTTAGATGGATCCGTGGTCGTCACATGTACAGGAAATTGTTGAGATCACCCAAAAAGAAGAGCAGAAGATTAGAGATTCCTGATTCTTCACCTTCTGAATGTGCTCCTAATGAAGTCGTTAACGAGTTTCAGAGCAATGAGGACTATAAGCTACCGAGGCCTGGTTCCAGAAACTCTACCTCGTCTTCACGCATTTCACCTGGCCTAGGTTCTAAAAGTGCATCACCATCAGATGGAAGGTTATATCCTTCTTACATCCACACTACACCTGAAAGGAGAGCAATGACAAAACATGAGTGGGACAGTCTCACTAGACACAACAGAGAAGGCCTTGGAAGAACTTGCTTCTTCTCCTGA

Protein Analysis

243

Amino Acids

27.32

Weight (kDa)

10.27

Isoelectric Point (pI)

59.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 6 - 76 1.1e-06 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 214
AccB1I GGYRCC 1 cut(s) 214
AccB7I CCANNNNNTGG 1 cut(s) 608
AclWI GGATC 3 cut(s) 109, 379, 392
AcoI YGGCCR 1 cut(s) 15
AcuI CTGAAG 1 cut(s) 345
AfaI GTAC 2 cut(s) 216, 404
AfiI CCNNNNNNNGG 3 cut(s) 536, 581, 608
AflIII ACRYGT 1 cut(s) 399
AgsI TTSAA 1 cut(s) 91
AhdI GACNNNNNGTC 1 cut(s) 308
AjnI CCWGG 2 cut(s) 535, 574
AluBI AGCT 3 cut(s) 155, 206, 526
AluI AGCT 3 cut(s) 155, 206, 526
Alw21I GWGCWC 1 cut(s) 481
Alw26I GTCTC 1 cut(s) 686
AlwI GGATC 3 cut(s) 109, 379, 392
AoxI GGCC 5 cut(s) 15, 84, 533, 577, 703
ApeKI GCWGC 1 cut(s) 197
Asp718I GGTACC 1 cut(s) 214
AspA2I CCTAGG 1 cut(s) 580
AspS9I GGNCC 2 cut(s) 138, 302
AsuHPI GGTGA 4 cut(s) 414, 456, 564, 591
AvaII GGWCC 2 cut(s) 138, 302
AvrII CCTAGG 1 cut(s) 580
BalI TGGCCA 1 cut(s) 17
BamHI GGATCC 1 cut(s) 384
BanI GGYRCC 1 cut(s) 214
BbsI GAAGAC 1 cut(s) 552
Bbv12I GWGCWC 1 cut(s) 481
BbvI GCAGC 1 cut(s) 209
BccI CCATC 4 cut(s) 92, 375, 602, 610
BciT130I CCWGG 2 cut(s) 537, 576
BcoDI GTCTC 1 cut(s) 686
BfaI CTAG 3 cut(s) 114, 581, 687
BfmI CTRYAG 2 cut(s) 306, 336
BisI GCNGC 1 cut(s) 198
BlnI CCTAGG 1 cut(s) 580
BlsI GCNGC 1 cut(s) 199
Bme1390I CCNGG 2 cut(s) 537, 576
Bme18I GGWCC 2 cut(s) 138, 302
BmeRI GACNNNNNGTC 1 cut(s) 308
BmgT120I GGNCC 2 cut(s) 138, 302
BmiI GGNNCC 3 cut(s) 216, 386, 541
BmrFI CCNGG 2 cut(s) 537, 576
BmsI GCATC 2 cut(s) 338, 605
BpiI GAAGAC 1 cut(s) 552
BpmI CTGGAG 1 cut(s) 69
BsaJI CCNNGG 4 cut(s) 388, 530, 580, 706
Bsc4I CCNNNNNNNGG 3 cut(s) 536, 581, 608
Bse1I ACTGG 1 cut(s) 198
Bse3DI GCAATG 2 cut(s) 517, 663
BseBI CCWGG 2 cut(s) 537, 576
BseDI CCNNGG 4 cut(s) 388, 530, 580, 706
BseGI GGATG 2 cut(s) 353, 630
BseLI CCNNNNNNNGG 3 cut(s) 536, 581, 608
BseMI GCAATG 2 cut(s) 517, 663
BseNI ACTGG 1 cut(s) 198
BseXI GCAGC 1 cut(s) 209
BshFI GGCC 5 cut(s) 17, 86, 535, 579, 705
BshNI GGYRCC 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 481
BslFI GGGAC 3 cut(s) 209, 254, 689
BslI CCNNNNNNNGG 3 cut(s) 536, 581, 608
BsmAI GTCTC 1 cut(s) 686
BsmFI GGGAC 3 cut(s) 209, 254, 689
BsnI GGCC 5 cut(s) 17, 86, 535, 579, 705
Bsp1286I GDGCHC 1 cut(s) 481
Bsp1407I TGTACA 1 cut(s) 402
Bsp143I GATC 4 cut(s) 101, 184, 384, 419
BspANI GGCC 5 cut(s) 17, 86, 535, 579, 705
BspLI GGNNCC 3 cut(s) 216, 386, 541
BspPI GGATC 3 cut(s) 109, 379, 392
BspQI GCTCTTC 1 cut(s) 428
BspT107I GGYRCC 1 cut(s) 214
BsrDI GCAATG 2 cut(s) 517, 663
BsrGI TGTACA 1 cut(s) 402
BsrI ACTGG 1 cut(s) 198
BssECI CCNNGG 4 cut(s) 388, 530, 580, 706
BssMI GATC 4 cut(s) 101, 184, 384, 419
BssT1I CCWWGG 2 cut(s) 580, 706
Bst2UI CCWGG 2 cut(s) 537, 576
Bst4CI ACNGT 2 cut(s) 73, 680
Bst6I CTCTTC 2 cut(s) 364, 428
BstAUI TGTACA 1 cut(s) 402
BstDSI CCRYGG 1 cut(s) 388
BstENI CCTNNNNNAGG 1 cut(s) 579
BstF5I GGATG 2 cut(s) 353, 630
BstKTI GATC 4 cut(s) 104, 187, 387, 422
BstMAI GTCTC 1 cut(s) 686
BstMBI GATC 4 cut(s) 101, 184, 384, 419
BstMWI GCNNNNNNNGC 2 cut(s) 203, 532
BstNI CCWGG 2 cut(s) 537, 576
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 2 cut(s) 535, 574
BstSFI CTRYAG 2 cut(s) 306, 336
BstV1I GCAGC 1 cut(s) 209
BstV2I GAAGAC 1 cut(s) 552
BstX2I RGATCY 1 cut(s) 384
BstYI RGATCY 1 cut(s) 384
BsuRI GGCC 5 cut(s) 17, 86, 535, 579, 705
BtgI CCRYGG 1 cut(s) 388
BtsCI GGATG 2 cut(s) 353, 630
Cfr13I GGNCC 2 cut(s) 138, 302
Csp6I GTAC 2 cut(s) 215, 403
CviAII CATG 3 cut(s) 54, 400, 668
CviQI GTAC 2 cut(s) 215, 403
DpnI GATC 4 cut(s) 103, 186, 386, 421
DpnII GATC 4 cut(s) 101, 184, 384, 419
DriI GACNNNNNGTC 1 cut(s) 308
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 2 cut(s) 364, 428
Eam1105I GACNNNNNGTC 1 cut(s) 308
EarI CTCTTC 2 cut(s) 364, 428
Eco130I CCWWGG 2 cut(s) 580, 706
Eco147I AGGCCT 2 cut(s) 535, 705
Eco47I GGWCC 2 cut(s) 138, 302
Eco57I CTGAAG 1 cut(s) 345
EcoNI CCTNNNNNAGG 1 cut(s) 579
EcoRII CCWGG 2 cut(s) 535, 574
EcoT14I CCWWGG 2 cut(s) 580, 706
EcoT22I ATGCAT 1 cut(s) 353
ErhI CCWWGG 2 cut(s) 580, 706
FaeI CATG 3 cut(s) 57, 403, 671
FaqI GGGAC 3 cut(s) 209, 254, 689
FatI CATG 3 cut(s) 53, 399, 667
Fnu4HI GCNGC 1 cut(s) 198
FokI GGATG 2 cut(s) 360, 617
Fsp4HI GCNGC 1 cut(s) 198
FspBI CTAG 3 cut(s) 114, 581, 687
GluI GCNGC 1 cut(s) 198
GsuI CTGGAG 1 cut(s) 69
HaeIII GGCC 5 cut(s) 17, 86, 535, 579, 705
Hin1II CATG 3 cut(s) 57, 403, 671
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HinfI GANTC 4 cut(s) 63, 110, 451, 458
HpaI GTTAAC 1 cut(s) 496
HphI GGTGA 4 cut(s) 414, 456, 564, 591
Hpy166II GTNNAC 2 cut(s) 138, 496
Hpy188I TCNGA 4 cut(s) 260, 472, 507, 607
Hpy188III TCNNGA 5 cut(s) 67, 280, 455, 543, 729
Hpy8I GTNNAC 2 cut(s) 138, 496
HpyAV CCTTC 4 cut(s) 477, 606, 633, 695
HpyCH4III ACNGT 2 cut(s) 73, 680
HpyCH4V TGCA 2 cut(s) 351, 596
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 532
Hsp92II CATG 3 cut(s) 57, 403, 671
KpnI GGTACC 1 cut(s) 218
KspAI GTTAAC 1 cut(s) 496
Kzo9I GATC 4 cut(s) 101, 184, 384, 419
LguI GCTCTTC 1 cut(s) 428
LmnI GCTCC 3 cut(s) 50, 203, 484
Lsp1109I GCAGC 1 cut(s) 209
LweI GCATC 2 cut(s) 338, 605
MaeI CTAG 3 cut(s) 114, 581, 687
MaeIII GTNAC 1 cut(s) 395
MalI GATC 4 cut(s) 103, 186, 386, 421
MboI GATC 4 cut(s) 101, 184, 384, 419
MboII GAAGA 9 cut(s) 17, 107, 351, 445, 453, 453, 552, 715, 723
MfeI CAATTG 1 cut(s) 105
MflI RGATCY 1 cut(s) 384
MhlI GDGCHC 1 cut(s) 481
MlsI TGGCCA 1 cut(s) 17
MluCI AATT 3 cut(s) 105, 243, 410
MluNI TGGCCA 1 cut(s) 17
MnlI CCTC 5 cut(s) 231, 318, 508, 525, 565
Mox20I TGGCCA 1 cut(s) 17
Mph1103I ATGCAT 1 cut(s) 353
MscI TGGCCA 1 cut(s) 17
MseI TTAA 2 cut(s) 275, 495
Msp20I TGGCCA 1 cut(s) 17
MspR9I CCNGG 2 cut(s) 537, 576
MunI CAATTG 1 cut(s) 105
MvaI CCWGG 2 cut(s) 537, 576
MwoI GCNNNNNNNGC 2 cut(s) 203, 532
NdeII GATC 4 cut(s) 101, 184, 384, 419
NlaIII CATG 3 cut(s) 57, 403, 671
NlaIV GGNNCC 3 cut(s) 216, 386, 541
NmuCI GTSAC 1 cut(s) 395
NsiI ATGCAT 1 cut(s) 353
NspI RCATGY 1 cut(s) 403
PceI AGGCCT 2 cut(s) 535, 705
PciI ACATGT 1 cut(s) 399
PciSI GCTCTTC 1 cut(s) 428
PfeI GAWTC 4 cut(s) 63, 110, 451, 458
PflMI CCANNNNNTGG 1 cut(s) 608
PkrI GCNGC 1 cut(s) 199
PscI ACATGT 1 cut(s) 399
Psp6I CCWGG 2 cut(s) 535, 574
PspGI CCWGG 2 cut(s) 535, 574
PspN4I GGNNCC 3 cut(s) 216, 386, 541
PspPI GGNCC 2 cut(s) 138, 302
PsuI RGATCY 1 cut(s) 384
RsaI GTAC 2 cut(s) 216, 404
RsaNI GTAC 2 cut(s) 215, 403
SapI GCTCTTC 1 cut(s) 428
SaqAI TTAA 2 cut(s) 275, 495
SatI GCNGC 1 cut(s) 198
Sau3AI GATC 4 cut(s) 101, 184, 384, 419
Sau96I GGNCC 2 cut(s) 138, 302
ScrFI CCNGG 2 cut(s) 537, 576
SduI GDGCHC 1 cut(s) 481
SfaNI GCATC 2 cut(s) 338, 605
SfcI CTRYAG 2 cut(s) 306, 336
SinI GGWCC 2 cut(s) 138, 302
Sse9I AATT 3 cut(s) 105, 243, 410
SseBI AGGCCT 2 cut(s) 535, 705
SspMI CTAG 3 cut(s) 114, 581, 687
StuI AGGCCT 2 cut(s) 535, 705
StyD4I CCNGG 2 cut(s) 535, 574
StyI CCWWGG 2 cut(s) 580, 706
TaaI ACNGT 2 cut(s) 73, 680
TasI AATT 3 cut(s) 105, 243, 410
TatI WGTACW 1 cut(s) 402
TfiI GAWTC 4 cut(s) 63, 110, 451, 458
Tru1I TTAA 2 cut(s) 275, 495
Tru9I TTAA 2 cut(s) 275, 495
TseFI GTSAC 1 cut(s) 395
TseI GCWGC 1 cut(s) 197
Tsp45I GTSAC 1 cut(s) 395
TspDTI ATGAA 2 cut(s) 307, 501
TspGWI ACGGA 1 cut(s) 377
Van91I CCANNNNNTGG 1 cut(s) 608
VpaK11BI GGWCC 2 cut(s) 138, 302
XagI CCTNNNNNAGG 1 cut(s) 579
XceI RCATGY 1 cut(s) 403
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XmaJI CCTAGG 1 cut(s) 580
XspI CTAG 3 cut(s) 114, 581, 687
Zsp2I ATGCAT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.