MD03G1061400.v1.1
ERF Family

Gamma-interferon-inducible lysosomal thiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007317
Physical Location & Seq
Forward (+)
4945194 .. 4949406
4213 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1061400.v1.1.491

Sequence Viewer

Length: 669 bp
ATGGCTTCCTCTCGGCTCGAACGTAGAACCCTTTCTTTTTCTCTTTTGTCTTGCCTCTTCTTGGTTGTTGTGTTCACACCCCCTTCGTCTTCTACAGACAGAACTCCACCTTCGGATTCTAGTAAAGTTTCACTGGCTTTGTATTACGAGTCTCTTTGCCCCTACAGCCCCAACTTCATTGTGAATTACCTTGTCAAGCTCTTCAAAGACGAGTTTATCTCCATTGTTGATCTCAAGGTTTCTCTTTGGGGTAAGGCCAAGCTCAGAAGCAATGACACATTCACTTGCCAGAATGATCTTTTCCTTTCGTTAATTGCGCCAAGAGTTTGGTTTACGAGCACAAGTAGACCCAGTGGGAGTCGTGTTACGAGAAACTGGGCTTGGATTCAAAACCTATTACTGAATGCTACAGCAGTGGACATGCATGGTAAAGAGGACTACGAAAACTTTCTGAGCTATGTCTGCAATGCCTGTAACGGCACCACTGCGCTCAAGGCTTGCAGTAGATTTTCCCTCAGTACCATCCAAAACGAAAGTTCGAAATCTACGCGTTCAGTTGGCTACAACGAGGGAAATATGGCAGCAGTAAGAGGAAGAATAGGATCGACCATAACATCGTGGATCCGTCAAATGAACCTGGCAATTTTGATATGGACGTTTATGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04612 map04612
Pfam Domains
Protein Families
GILT

Protein Analysis

223

Amino Acids

24.95

Weight (kDa)

9.34

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GILT PF03227 45 - 98 8.1e-11 Gamma interferon inducible lysosomal thiol reductase (GILT)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 479
AccI GTMKAC 1 cut(s) 346
AccII CGCG 1 cut(s) 550
AclWI GGATC 3 cut(s) 610, 616, 629
AfaI GTAC 1 cut(s) 520
AfiI CCNNNNNNNGG 1 cut(s) 61
AflIII ACRYGT 1 cut(s) 548
AgsI TTSAA 2 cut(s) 205, 389
AjnI CCWGG 1 cut(s) 636
AjuI GAANNNNNNNTTGG 2 cut(s) 364, 396
AluBI AGCT 3 cut(s) 199, 262, 456
AluI AGCT 3 cut(s) 199, 262, 456
Alw21I GWGCWC 1 cut(s) 341
Alw26I GTCTC 1 cut(s) 156
AlwI GGATC 3 cut(s) 610, 616, 629
AoxI GGCC 1 cut(s) 255
ApeKI GCWGC 1 cut(s) 581
Asp700I GAANNNNTTC 2 cut(s) 31, 447
AspLEI GCGC 2 cut(s) 319, 490
AsuII TTCGAA 1 cut(s) 539
BamHI GGATCC 1 cut(s) 621
BanI GGYRCC 1 cut(s) 479
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 341
BbvI GCAGC 1 cut(s) 593
BccI CCATC 1 cut(s) 530
BceAI ACGGC 1 cut(s) 493
BciT130I CCWGG 1 cut(s) 638
BcoDI GTCTC 1 cut(s) 156
BfaI CTAG 1 cut(s) 120
BfmI CTRYAG 3 cut(s) 93, 163, 408
BisI GCNGC 1 cut(s) 582
BlsI GCNGC 1 cut(s) 583
Bme1390I CCNGG 1 cut(s) 638
BmiI GGNNCC 2 cut(s) 481, 623
BmrFI CCNGG 1 cut(s) 638
BmrI ACTGGG 2 cut(s) 345, 385
BmuI ACTGGG 2 cut(s) 345, 385
BpiI GAAGAC 1 cut(s) 81
BplI GAGNNNNNCTC 2 cut(s) 203, 235
Bpu14I TTCGAA 1 cut(s) 539
BpuEI CTTGAG 2 cut(s) 218, 476
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse1I ACTGG 3 cut(s) 138, 351, 380
Bse3DI GCAATG 2 cut(s) 277, 472
BseBI CCWGG 1 cut(s) 638
BseGI GGATG 1 cut(s) 522
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMI GCAATG 2 cut(s) 277, 472
BseMII CTCAG 3 cut(s) 277, 443, 529
BseNI ACTGG 3 cut(s) 138, 351, 380
BseXI GCAGC 1 cut(s) 593
Bsh1236I CGCG 1 cut(s) 550
BshFI GGCC 1 cut(s) 257
BshNI GGYRCC 1 cut(s) 479
BsiHKAI GWGCWC 1 cut(s) 341
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 1 cut(s) 156
BsmI GAATGC 1 cut(s) 409
BsnI GGCC 1 cut(s) 257
Bsp119I TTCGAA 1 cut(s) 539
Bsp1286I GDGCHC 1 cut(s) 341
Bsp143I GATC 4 cut(s) 229, 295, 602, 621
BspANI GGCC 1 cut(s) 257
BspCNI CTCAG 3 cut(s) 276, 444, 528
BspFNI CGCG 1 cut(s) 550
BspLI GGNNCC 2 cut(s) 481, 623
BspPI GGATC 3 cut(s) 610, 616, 629
BspQI GCTCTTC 1 cut(s) 206
BspT104I TTCGAA 1 cut(s) 539
BspT107I GGYRCC 1 cut(s) 479
BsrDI GCAATG 2 cut(s) 277, 472
BsrI ACTGG 3 cut(s) 138, 351, 380
BssMI GATC 4 cut(s) 229, 295, 602, 621
Bst2UI CCWGG 1 cut(s) 638
Bst6I CTCTTC 2 cut(s) 62, 206
BstBI TTCGAA 1 cut(s) 539
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 3 cut(s) 263, 452, 515
BstF5I GGATG 1 cut(s) 522
BstFNI CGCG 1 cut(s) 550
BstHHI GCGC 2 cut(s) 319, 490
BstKTI GATC 4 cut(s) 232, 298, 605, 624
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 4 cut(s) 229, 295, 602, 621
BstMWI GCNNNNNNNGC 3 cut(s) 165, 462, 494
BstNI CCWGG 1 cut(s) 638
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 1 cut(s) 636
BstSFI CTRYAG 3 cut(s) 93, 163, 408
BstUI CGCG 1 cut(s) 550
BstV1I GCAGC 1 cut(s) 593
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 1 cut(s) 621
BstXI CCANNNNNNTGG 1 cut(s) 327
BstYI RGATCY 1 cut(s) 621
BsuRI GGCC 1 cut(s) 257
BtsCI GGATG 1 cut(s) 522
BtsI GCAGTG 2 cut(s) 420, 483
BtsIMutI CAGTG 4 cut(s) 131, 358, 420, 483
Cac8I GCNNGC 1 cut(s) 499
CfoI GCGC 2 cut(s) 319, 490
Csp6I GTAC 1 cut(s) 519
CviAII CATG 2 cut(s) 421, 425
CviQI GTAC 1 cut(s) 519
DdeI CTNAG 3 cut(s) 263, 452, 515
DpnI GATC 4 cut(s) 231, 297, 604, 623
DpnII GATC 4 cut(s) 229, 295, 602, 621
Eam1104I CTCTTC 2 cut(s) 62, 206
EarI CTCTTC 2 cut(s) 62, 206
EcoRII CCWGG 1 cut(s) 636
EcoT22I ATGCAT 1 cut(s) 426
FaeI CATG 2 cut(s) 424, 428
FaiI YATR 7 cut(s) 422, 426, 459, 578, 611, 652, 662
FatI CATG 2 cut(s) 420, 424
FblI GTMKAC 1 cut(s) 346
Fnu4HI GCNGC 1 cut(s) 582
FokI GGATG 1 cut(s) 509
Fsp4HI GCNGC 1 cut(s) 582
FspBI CTAG 1 cut(s) 120
GlaI GCGC 2 cut(s) 318, 489
GluI GCNGC 1 cut(s) 582
HaeIII GGCC 1 cut(s) 257
HhaI GCGC 2 cut(s) 319, 490
Hin1II CATG 2 cut(s) 424, 428
Hin6I GCGC 2 cut(s) 317, 488
HinP1I GCGC 2 cut(s) 317, 488
HinfI GANTC 4 cut(s) 116, 149, 358, 385
Hpy166II GTNNAC 4 cut(s) 75, 333, 347, 418
Hpy188I TCNGA 3 cut(s) 115, 266, 453
Hpy8I GTNNAC 4 cut(s) 75, 333, 347, 418
HpyAV CCTTC 2 cut(s) 93, 120
HpyCH4IV ACGT 2 cut(s) 22, 656
HpyCH4V TGCA 3 cut(s) 424, 465, 501
HpyF10VI GCNNNNNNNGC 3 cut(s) 165, 462, 494
HpyF3I CTNAG 3 cut(s) 263, 452, 515
HpySE526I ACGT 2 cut(s) 22, 656
Hsp92II CATG 2 cut(s) 424, 428
HspAI GCGC 2 cut(s) 317, 488
Kzo9I GATC 4 cut(s) 229, 295, 602, 621
LguI GCTCTTC 1 cut(s) 206
LpnPI CCDG 7 cut(s) 119, 302, 361, 364, 484, 623, 650
Lsp1109I GCAGC 1 cut(s) 593
MaeI CTAG 1 cut(s) 120
MaeII ACGT 2 cut(s) 22, 656
MaeIII GTNAC 2 cut(s) 364, 473
MalI GATC 4 cut(s) 231, 297, 604, 623
MboI GATC 4 cut(s) 229, 295, 602, 621
MboII GAAGA 4 cut(s) 49, 81, 193, 606
MflI RGATCY 1 cut(s) 621
MhlI GDGCHC 1 cut(s) 341
MluCI AATT 3 cut(s) 184, 312, 642
MluI ACGCGT 1 cut(s) 548
MlyI GAGTC 2 cut(s) 158, 367
MnlI CCTC 6 cut(s) 19, 65, 427, 524, 562, 584
Mph1103I ATGCAT 1 cut(s) 426
MroXI GAANNNNTTC 2 cut(s) 31, 447
MseI TTAA 1 cut(s) 311
MspR9I CCNGG 1 cut(s) 638
Mva1269I GAATGC 1 cut(s) 409
MvaI CCWGG 1 cut(s) 638
MvnI CGCG 1 cut(s) 550
MwoI GCNNNNNNNGC 3 cut(s) 165, 462, 494
NdeII GATC 4 cut(s) 229, 295, 602, 621
NlaIII CATG 2 cut(s) 424, 428
NlaIV GGNNCC 2 cut(s) 481, 623
NsiI ATGCAT 1 cut(s) 426
NspI RCATGY 1 cut(s) 424
NspV TTCGAA 1 cut(s) 539
PciSI GCTCTTC 1 cut(s) 206
PcsI WCGNNNNNNNCGW 1 cut(s) 19
PctI GAATGC 1 cut(s) 409
PdmI GAANNNNTTC 2 cut(s) 31, 447
PfeI GAWTC 2 cut(s) 116, 385
PkrI GCNGC 1 cut(s) 583
PleI GAGTC 2 cut(s) 157, 366
PpsI GAGTC 2 cut(s) 157, 366
Psp6I CCWGG 1 cut(s) 636
PspGI CCWGG 1 cut(s) 636
PspN4I GGNNCC 2 cut(s) 481, 623
PsuI RGATCY 1 cut(s) 621
RsaI GTAC 1 cut(s) 520
RsaNI GTAC 1 cut(s) 519
SapI GCTCTTC 1 cut(s) 206
SaqAI TTAA 1 cut(s) 311
SatI GCNGC 1 cut(s) 582
Sau3AI GATC 4 cut(s) 229, 295, 602, 621
SchI GAGTC 2 cut(s) 158, 367
ScrFI CCNGG 1 cut(s) 638
SduI GDGCHC 1 cut(s) 341
SfcI CTRYAG 3 cut(s) 93, 163, 408
SfuI TTCGAA 1 cut(s) 539
SmlI CTYRAG 2 cut(s) 233, 491
SmoI CTYRAG 2 cut(s) 233, 491
Sse9I AATT 3 cut(s) 184, 312, 642
SspMI CTAG 1 cut(s) 120
StyD4I CCNGG 1 cut(s) 636
TaiI ACGT 2 cut(s) 25, 659
TaqI TCGA 3 cut(s) 18, 539, 605
TasI AATT 3 cut(s) 184, 312, 642
TfiI GAWTC 2 cut(s) 116, 385
Tru1I TTAA 1 cut(s) 311
Tru9I TTAA 1 cut(s) 311
TscAI CASTG 4 cut(s) 138, 358, 420, 490
TseI GCWGC 1 cut(s) 581
TspDTI ATGAA 2 cut(s) 166, 647
TspGWI ACGGA 1 cut(s) 614
TspRI CASTG 4 cut(s) 138, 358, 420, 490
XceI RCATGY 1 cut(s) 424
XmiI GTMKAC 1 cut(s) 346
XmnI GAANNNNTTC 2 cut(s) 31, 447
XspI CTAG 1 cut(s) 120
Zsp2I ATGCAT 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.