MD03G1064100.v1.1

N-acetyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012341
Physical Location & Seq
Forward (+)
5142593 .. 5144741
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1064100.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGGGATTTCAGGAGGCTATAATTCGAAGCTATGACGGGCTGGCGGATAGAGGAAGAGTGGAAGATCTCGAACGAAGATGTGAAGTAGGGCCATCCGAACGTGTGTTTCTCTTCACCGATACGATGGGTGACCCCATATGTAGGATCAGAAGCAGTCCGATGCACAAGATGCTGGTGGCTGAGTTGGACAACCAGTTGGTTGGGGTCATCCAGGGCTCTATAAAGGTGGTCACAGTTCATCACAACAAGCCACCACCTAATGATCAGGCCAAAGTTGGCTACATCTTAGGCCTCAGGGTTTCACCGCTGTATAGAAGAAAAGGAATTGGGTCAAACCTTGTGAGCAAATTAGAAGAATGGTTCATAGACAATGATGCTGATTATGCATACATGGCTACGGAGAAAGATAACCTTGCGTCAGTCAGACTTTTCATAACAAAGTTTGGCTACATCAGGTTCCGGACGCCGGCGATTCTGGTGAACCCGGTTCGTTACAGGCCCCGCCACGTGTCTTCCAACGTTGAGATTGCAAAGTTGAAGGTTGAAGAAGCTGAATATCTCTACAGAAAGTGTATGGAAAATACTGAGTTTTTTCCTCATGATATAGAAAAAGTGCTTGGGAATAAGCTGAGTTTGGGGACTTGGGTGGCTTACCCTAGAGGAGAGTTGAGAGATTTTGGGGTAAATGGTCATTTACCTGAAAGTTGGGCTATGCTTAGTGTGTGGAATAGTGGGGAGCTTTTTAACCTGAGGCTAGGGAATGCACCCTTATCTTGCTTAATGTATGCAAAAAGTTGGAAATGGATGGCTAGGGTTTTTCCTTGCTTTAAATTACTGCCTTCCATGCCTGATTTTTTTATCCCTTTTGGATTTTACTTCATGTATGGGTTGCACCATGAAGGACCATTGTCAGGGAAATTGGTGAGGGCTCTCTGCCAGTTTGTGCACAACATGGCTGCTACCACTTCCGAGAATTGCAAGGTTATAGTGACAGAAGTTGGAGGCTGTGATCCAGTGAGACATCACATCCCACATTGGAAATTGCTCTCATGTTATGAAGATTTGTGGTGCATAAAGGCCTTGAAAAGTGAAGATAGAAGAACTAATAGTGTCCATGAATTCACAAGAACCCACCCAACAAAATCTCTCTTTGTAGATCCAAGAGAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1

Protein Analysis

391

Amino Acids

44.81

Weight (kDa)

8.7

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acetyltransf_1 PF00583 45 - 150 1.9e-16 Acetyltransferase (GNAT) family
Acetyltransf_7 PF13508 55 - 151 1.1e-07 Acetyltransferase (GNAT) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30090
fragaria_vesca FvH4_3g39120 FvH4_3g39120
malus_domestica MD03G1064100.v1.1 MD11G1067900.v1.1
prunus_persica Prupe.6G053400_v2.0.a1
pyrus_communis pycom03g05160 pycom11g05700
rosa_chinensis RchiOBHm_Chr5g0070491
rosa_laevigata RLG00000036137
rosa_multiflora Rmu_sc0005708.1_g000009
rosa_roxburghii Rroxscaffold_1G00010740
rosa_rugosa Rorug05G0406200 Rorug05G0406300
rosa_samantha Rh5AG461400 Rh5BG479700 Rh5CG503900 Rh5DG492000
rosa_wichuraiana Rw5G043020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 459
AciI CCGC 3 cut(s) 44, 305, 502
AclI AACGTT 1 cut(s) 519
AclWI GGATC 3 cut(s) 152, 1004, 1151
AcsI RAATTY 1 cut(s) 1118
AcvI CACGTG 1 cut(s) 508
AcyI GRCGYC 1 cut(s) 464
AfiI CCNNNNNNNGG 3 cut(s) 141, 466, 911
AflIII ACRYGT 2 cut(s) 100, 507
AgsI TTSAA 3 cut(s) 538, 545, 1084
AjnI CCWGG 1 cut(s) 210
AjuI GAANNNNNNNTTGG 2 cut(s) 600, 632
AluBI AGCT 4 cut(s) 30, 551, 628, 739
AluI AGCT 4 cut(s) 30, 551, 628, 739
Alw21I GWGCWC 1 cut(s) 948
Alw26I GTCTC 1 cut(s) 1012
Alw44I GTGCAC 1 cut(s) 944
AlwI GGATC 3 cut(s) 152, 1004, 1151
Aor13HI TCCGGA 1 cut(s) 459
AoxI GGCC 5 cut(s) 89, 267, 289, 497, 1077
ApaLI GTGCAC 1 cut(s) 944
ApeKI GCWGC 1 cut(s) 956
ApoI RAATTY 1 cut(s) 1118
AspS9I GGNCC 3 cut(s) 89, 498, 902
AsuC2I CCSGG 1 cut(s) 485
AsuHPI GGTGA 5 cut(s) 106, 140, 294, 490, 934
AsuII TTCGAA 1 cut(s) 25
AvaII GGWCC 1 cut(s) 902
AxyI CCTNAGG 2 cut(s) 293, 749
BaeGI GKGCMC 1 cut(s) 948
BanII GRGCYC 2 cut(s) 218, 931
BbrPI CACGTG 1 cut(s) 508
BbsI GAAGAC 1 cut(s) 504
Bbv12I GWGCWC 1 cut(s) 948
BbvI GCAGC 1 cut(s) 943
BccI CCATC 3 cut(s) 100, 118, 799
BciT130I CCWGG 1 cut(s) 212
BclI TGATCA 1 cut(s) 262
BcnI CCSGG 1 cut(s) 485
BcoDI GTCTC 1 cut(s) 1012
BfaI CTAG 3 cut(s) 657, 755, 810
BfmI CTRYAG 1 cut(s) 562
BglII AGATCT 1 cut(s) 64
BisI GCNGC 1 cut(s) 957
BlsI GCNGC 1 cut(s) 958
Bme1390I CCNGG 2 cut(s) 212, 485
Bme18I GGWCC 1 cut(s) 902
BmgT120I GGNCC 3 cut(s) 89, 498, 902
BmiI GGNNCC 2 cut(s) 458, 500
BmrFI CCNGG 2 cut(s) 212, 485
BmsI GCATC 3 cut(s) 150, 159, 364
BoxI GACNNNNGTC 1 cut(s) 907
BpiI GAAGAC 1 cut(s) 504
Bpu14I TTCGAA 1 cut(s) 25
BpuMI CCSGG 1 cut(s) 485
BsaAI YACGTR 1 cut(s) 508
BsaHI GRCGYC 1 cut(s) 464
BsaJI CCNNGG 1 cut(s) 211
BsaWI WCCGGW 1 cut(s) 459
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 466, 911
Bse118I RCCGGY 1 cut(s) 466
Bse1I ACTGG 3 cut(s) 193, 937, 1013
Bse21I CCTNAGG 2 cut(s) 293, 749
BseAI TCCGGA 1 cut(s) 459
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 1 cut(s) 211
BseGI GGATG 4 cut(s) 92, 207, 810, 1026
BseLI CCNNNNNNNGG 3 cut(s) 141, 466, 911
BseMII CTCAG 5 cut(s) 171, 307, 576, 620, 740
BseNI ACTGG 3 cut(s) 193, 937, 1013
BseRI GAGGAG 1 cut(s) 675
BseSI GKGCMC 1 cut(s) 948
BseXI GCAGC 1 cut(s) 943
BshFI GGCC 5 cut(s) 91, 269, 291, 499, 1079
BsiHKAI GWGCWC 1 cut(s) 948
BsiSI CCGG 3 cut(s) 460, 467, 485
BslFI GGGAC 1 cut(s) 652
BslI CCNNNNNNNGG 3 cut(s) 141, 466, 911
BsmAI GTCTC 1 cut(s) 1012
BsmFI GGGAC 1 cut(s) 652
BsmI GAATGC 1 cut(s) 766
BsnI GGCC 5 cut(s) 91, 269, 291, 499, 1079
Bsp119I TTCGAA 1 cut(s) 25
Bsp1286I GDGCHC 3 cut(s) 218, 931, 948
Bsp13I TCCGGA 1 cut(s) 459
Bsp143I GATC 5 cut(s) 64, 144, 262, 1009, 1156
BspACI CCGC 3 cut(s) 44, 305, 502
BspANI GGCC 5 cut(s) 91, 269, 291, 499, 1079
BspCNI CTCAG 5 cut(s) 172, 306, 577, 621, 741
BspEI TCCGGA 1 cut(s) 459
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 2 cut(s) 458, 500
BspPI GGATC 3 cut(s) 152, 1004, 1151
BspT104I TTCGAA 1 cut(s) 25
BsrFI RCCGGY 1 cut(s) 466
BsrI ACTGG 3 cut(s) 193, 937, 1013
BssAI RCCGGY 1 cut(s) 466
BssECI CCNNGG 1 cut(s) 211
BssMI GATC 5 cut(s) 64, 144, 262, 1009, 1156
BssNI GRCGYC 1 cut(s) 464
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 1 cut(s) 235
Bst6I CTCTTC 2 cut(s) 49, 116
BstACI GRCGYC 1 cut(s) 464
BstAPI GCANNNNNTGC 1 cut(s) 169
BstBAI YACGTR 1 cut(s) 508
BstBI TTCGAA 1 cut(s) 25
BstC8I GCNNGC 2 cut(s) 42, 468
BstDEI CTNAG 7 cut(s) 180, 286, 293, 585, 629, 716, 749
BstEII GGTNACC 1 cut(s) 128
BstF5I GGATG 4 cut(s) 92, 207, 810, 1026
BstKTI GATC 5 cut(s) 67, 147, 265, 1012, 1159
BstMAI GTCTC 1 cut(s) 1012
BstMBI GATC 5 cut(s) 64, 144, 262, 1009, 1156
BstMWI GCNNNNNNNGC 4 cut(s) 169, 383, 392, 844
BstNI CCWGG 1 cut(s) 212
BstPAI GACNNNNGTC 1 cut(s) 907
BstPI GGTNACC 1 cut(s) 128
BstSCI CCNGG 2 cut(s) 210, 483
BstSFI CTRYAG 1 cut(s) 562
BstSLI GKGCMC 1 cut(s) 948
BstV1I GCAGC 1 cut(s) 943
BstV2I GAAGAC 1 cut(s) 504
BstX2I RGATCY 2 cut(s) 64, 1156
BstXI CCANNNNNNTGG 1 cut(s) 200
BstYI RGATCY 2 cut(s) 64, 1156
Bsu36I CCTNAGG 2 cut(s) 293, 749
BsuRI GGCC 5 cut(s) 91, 269, 291, 499, 1079
BtsCI GGATG 4 cut(s) 92, 207, 810, 1026
BtsIMutI CAGTG 1 cut(s) 1020
Cac8I GCNNGC 2 cut(s) 42, 468
CciI TCATGA 1 cut(s) 598
Cfr10I RCCGGY 1 cut(s) 466
Cfr13I GGNCC 3 cut(s) 89, 498, 902
CseI GACGC 2 cut(s) 405, 472
CviAII CATG 8 cut(s) 391, 599, 844, 880, 896, 952, 1050, 1115
DdeI CTNAG 7 cut(s) 180, 286, 293, 585, 629, 716, 749
DpnI GATC 5 cut(s) 66, 146, 264, 1011, 1158
DpnII GATC 5 cut(s) 64, 144, 262, 1009, 1156
DraI TTTAAA 1 cut(s) 829
Eam1104I CTCTTC 2 cut(s) 49, 116
EarI CTCTTC 2 cut(s) 49, 116
EciI GGCGGA 1 cut(s) 59
Eco147I AGGCCT 2 cut(s) 291, 1079
Eco24I GRGCYC 2 cut(s) 218, 931
Eco47I GGWCC 1 cut(s) 902
Eco72I CACGTG 1 cut(s) 508
Eco81I CCTNAGG 2 cut(s) 293, 749
Eco91I GGTNACC 1 cut(s) 128
EcoO109I RGGNCCY 1 cut(s) 498
EcoO65I GGTNACC 1 cut(s) 128
EcoRI GAATTC 1 cut(s) 1118
EcoRII CCWGG 1 cut(s) 210
EcoT22I ATGCAT 1 cut(s) 388
EcoT38I GRGCYC 2 cut(s) 218, 931
FaeI CATG 8 cut(s) 394, 602, 847, 883, 899, 955, 1053, 1118
FalI AAGNNNNNCTT 2 cut(s) 396, 428
FaqI GGGAC 1 cut(s) 652
FatI CATG 8 cut(s) 390, 598, 843, 879, 895, 951, 1049, 1114
FauI CCCGC 1 cut(s) 509
FauNDI CATATG 1 cut(s) 137
FbaI TGATCA 1 cut(s) 262
Fnu4HI GCNGC 1 cut(s) 957
FokI GGATG 4 cut(s) 79, 194, 817, 1013
FriOI GRGCYC 2 cut(s) 218, 931
Fsp4HI GCNGC 1 cut(s) 957
FspBI CTAG 3 cut(s) 657, 755, 810
GluI GCNGC 1 cut(s) 957
HaeIII GGCC 5 cut(s) 91, 269, 291, 499, 1079
HapII CCGG 3 cut(s) 460, 467, 485
HgaI GACGC 2 cut(s) 405, 472
Hin1I GRCGYC 1 cut(s) 464
Hin1II CATG 8 cut(s) 394, 602, 847, 883, 899, 955, 1053, 1118
HinfI GANTC 1 cut(s) 472
HpaII CCGG 3 cut(s) 460, 467, 485
HphI GGTGA 5 cut(s) 106, 140, 294, 490, 934
Hpy166II GTNNAC 2 cut(s) 481, 946
Hpy188I TCNGA 5 cut(s) 97, 149, 159, 425, 970
Hpy188III TCNNGA 4 cut(s) 11, 68, 460, 599
Hpy8I GTNNAC 2 cut(s) 481, 946
HpyAV CCTTC 3 cut(s) 532, 849, 893
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4IV ACGT 3 cut(s) 100, 507, 519
HpyCH4V TGCA 9 cut(s) 163, 386, 530, 764, 788, 892, 946, 978, 1071
HpyF10VI GCNNNNNNNGC 4 cut(s) 169, 383, 392, 844
HpyF3I CTNAG 7 cut(s) 180, 286, 293, 585, 629, 716, 749
HpySE526I ACGT 3 cut(s) 100, 507, 519
Hsp92I GRCGYC 1 cut(s) 464
Hsp92II CATG 8 cut(s) 394, 602, 847, 883, 899, 955, 1053, 1118
Kpn2I TCCGGA 1 cut(s) 459
KroI GCCGGC 1 cut(s) 466
KroNI GCCGGC 1 cut(s) 468
Ksp22I TGATCA 1 cut(s) 262
Kzo9I GATC 5 cut(s) 64, 144, 262, 1009, 1156
LmnI GCTCC 1 cut(s) 736
Lsp1109I GCAGC 1 cut(s) 943
LweI GCATC 3 cut(s) 150, 159, 364
MaeI CTAG 3 cut(s) 657, 755, 810
MaeII ACGT 3 cut(s) 100, 507, 519
MaeIII GTNAC 4 cut(s) 128, 229, 491, 988
MalI GATC 5 cut(s) 66, 146, 264, 1011, 1158
MboI GATC 5 cut(s) 64, 144, 262, 1009, 1156
MflI RGATCY 2 cut(s) 64, 1156
MhlI GDGCHC 3 cut(s) 218, 931, 948
MluCI AATT 8 cut(s) 21, 324, 347, 830, 917, 973, 1040, 1118
MmeI TCCRAC 4 cut(s) 165, 540, 776, 979
MnlI CCTC 9 cut(s) 7, 44, 302, 606, 653, 744, 918, 995, 1159
Mph1103I ATGCAT 1 cut(s) 388
MreI CGCCGGCG 1 cut(s) 466
MroI TCCGGA 1 cut(s) 459
MroNI GCCGGC 1 cut(s) 466
MseI TTAA 3 cut(s) 744, 779, 828
MspA1I CMGCKG 1 cut(s) 307
MspI CCGG 3 cut(s) 460, 467, 485
MspR9I CCNGG 2 cut(s) 212, 485
Mva1269I GAATGC 1 cut(s) 766
MvaI CCWGG 1 cut(s) 212
MwoI GCNNNNNNNGC 4 cut(s) 169, 383, 392, 844
NaeI GCCGGC 1 cut(s) 468
NciI CCSGG 1 cut(s) 485
NdeI CATATG 1 cut(s) 137
NdeII GATC 5 cut(s) 64, 144, 262, 1009, 1156
NgoMIV GCCGGC 1 cut(s) 466
NlaIII CATG 8 cut(s) 394, 602, 847, 883, 899, 955, 1053, 1118
NlaIV GGNNCC 2 cut(s) 458, 500
NmuCI GTSAC 3 cut(s) 128, 229, 988
NsiI ATGCAT 1 cut(s) 388
NspV TTCGAA 1 cut(s) 25
PagI TCATGA 1 cut(s) 598
PceI AGGCCT 2 cut(s) 291, 1079
PctI GAATGC 1 cut(s) 766
PdiI GCCGGC 1 cut(s) 468
PfeI GAWTC 1 cut(s) 472
PkrI GCNGC 1 cut(s) 958
PmaCI CACGTG 1 cut(s) 508
PmlI CACGTG 1 cut(s) 508
Ppu21I YACGTR 1 cut(s) 508
PshAI GACNNNNGTC 1 cut(s) 907
Psp1406I AACGTT 1 cut(s) 519
Psp6I CCWGG 1 cut(s) 210
PspCI CACGTG 1 cut(s) 508
PspEI GGTNACC 1 cut(s) 128
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 2 cut(s) 458, 500
PspPI GGNCC 3 cut(s) 89, 498, 902
PsuI RGATCY 2 cut(s) 64, 1156
SaqAI TTAA 3 cut(s) 744, 779, 828
SatI GCNGC 1 cut(s) 957
Sau3AI GATC 5 cut(s) 64, 144, 262, 1009, 1156
Sau96I GGNCC 3 cut(s) 89, 498, 902
ScrFI CCNGG 2 cut(s) 212, 485
SduI GDGCHC 3 cut(s) 218, 931, 948
SfaNI GCATC 3 cut(s) 150, 159, 364
SfcI CTRYAG 1 cut(s) 562
SfuI TTCGAA 1 cut(s) 25
SgrAI CRCCGGYG 1 cut(s) 466
SinI GGWCC 1 cut(s) 902
Sse9I AATT 8 cut(s) 21, 324, 347, 830, 917, 973, 1040, 1118
SseBI AGGCCT 2 cut(s) 291, 1079
SsiI CCGC 3 cut(s) 44, 305, 502
SspMI CTAG 3 cut(s) 657, 755, 810
StuI AGGCCT 2 cut(s) 291, 1079
StyD4I CCNGG 2 cut(s) 210, 483
TaaI ACNGT 1 cut(s) 235
TaiI ACGT 3 cut(s) 103, 510, 522
TaqI TCGA 2 cut(s) 25, 69
TasI AATT 8 cut(s) 21, 324, 347, 830, 917, 973, 1040, 1118
TfiI GAWTC 1 cut(s) 472
Tru1I TTAA 3 cut(s) 744, 779, 828
Tru9I TTAA 3 cut(s) 744, 779, 828
TscAI CASTG 1 cut(s) 1020
TseFI GTSAC 3 cut(s) 128, 229, 988
TseI GCWGC 1 cut(s) 956
Tsp45I GTSAC 3 cut(s) 128, 229, 988
TspDTI ATGAA 7 cut(s) 227, 352, 421, 868, 912, 1071, 1131
TspGWI ACGGA 1 cut(s) 413
TspRI CASTG 1 cut(s) 1020
VneI GTGCAC 1 cut(s) 944
VpaK11BI GGWCC 1 cut(s) 902
XapI RAATTY 1 cut(s) 1118
XspI CTAG 3 cut(s) 657, 755, 810
Zsp2I ATGCAT 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.