Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0011766

Physical Location & Seq

Genomic Coordinates Reverse (-)

5557849 .. 5561283

Gene Structure 3435 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1068400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2124 bp

ATGTGCAGTGGTTCCAAGACGAAACCTTCCTCCACGGATATGGTCATGGAGCATGAGAAACAAGAAGGGATGCTGTATAGCTTTTCGATTTTGCTTGAACTAGCAGCCTGCGATGATCTTGAAGGGCTCAAAAGGGCTGTCGAAGAAGAGGGTCATGATGTTGATGAGGCGAGCTATTGGTACGGGAGGCTGATTGGATCGAAGAAGTTGGGGTATGAGGAGAGGACGCCCCTCATGATCGCTGCTATGTTCGGTAGCAAGAACGTGTTGAATTATATTCTTCAATTGTGTATTGTTAATGTTAATAGAGCCTGCGGTTCAGATAGAGCCACTGCTCTTCATTGTGCTGTTGCTGGTGGCTCTGCTGCTTTGGCGGAGGTTGTCAAGCTCTTGCTTGCTGCTTCTGCTGATTCGAGTGCTCTTGATGCTAATGGAAACCAAGCTGGTGACTTGATCACATCTGCATATGGTTCGAGCTTCAATTTGAGGAAGAAAGCTTTGGAGGGCATGCTCAAGGGTGTTCCCAGTATTGATGAACCTTTTGACTTCTCGGAGCAAATGATTAATGAGACAGAAGGTCAAGAACAGAAAGAGATTACAACTCCTCGTGCTTCAAAAGATGGAACGGAGAGGAAAGAGTATCCTGTTGATCTCTCTCTTCCAGATATCAAGAATGGGATTTATAGCACAGATGAGTTTAGAATGTATACTTTCAAGGTTAAGCCTTGCTCTAGGGCTTACTCACACGACTGGACAGAGTGCCCATTTGTCCACCCTGGAGAAAATGCGAGGCGGCGTGATCCAAGGAAATATCATTACAGCTGTGTTCCTTGCCCGGAGTTCAGAAAGGGGACCTGCAGGCAGGGAGATGATTGTGAATATGCACATGGTATTTTTGAATGTTGGCTTCACCCTGCCCAGTACCGCACTCGTCTTTGTAAGGATGAGACCGGATGCACAAGAAGGGTATGTTTCTTTGCTCACAAGCCAGAAGAGCTTCGTCCCTTGTATGCTTCGACAGGTTCTGCTGTGCCTTCTCCGAGGTCATTCTCAGCCAGTGGTGCTTCTCTGGATATGGGGTCAATTACCCCACTTTCCCTCAATTCTCCATCCATGATGATTCCTCCTACTTCAACTCCACCCATGACTCCCACTGGACCTTCTTCTCCTAGGAGTGGTTTCATGTGGCAAAACAATCCAAACTTTGCACCCCCTACCTTGCAGCTCCCAGGTAGCAGGTTGAAATCTACTCTAAGTGCTAGAGATATGGATTTTGACATTGAAATGCTTAGCCTGGAGAAGGATCGCCGAAGGCAGCAACGCTTGATAGATGAGATATCTGGTTCCCCATCTAACTGGAACAATGGCTTATCTCCTGCGTCACCTTTTTCTGCCTCTGGGAATCGAACAGGGGAATTGAATAGCCTGGGAGGAGTGAACCCTACTAACCTTGACGACATTTTTGGATCTGTTGAACCTGCAATTTTGCCTCAATTTAATGGCCTTTCGCGTGATTCAACAGCATCCCAGTTACATTCTCCGACTGGGATGCGCCAAAACATGAACCTGCAGGCCCGTCCCAGTTACTCTGCCAGCCTCTCATCCTCTCCTGGAAGGGCTTCTTCAATGTTTGGAGTTGATGCATCTAGTGCAGCTGCTGTTTTTAGTTCAAGGTCAGCTGCATTTGCAAACAGGAGTCAGAGTTTCATTGAGCGTAGTGCCGGCAACCGTAACACTGGAGTTTCTTCTTCTGCTGATTTTGGAACATTAAAGCCCTCTAACCTTTCAGATTGGGGCTCACCTGGTGGCAAATTGGACTGGGGTATGCAGGGGGAAGAGCTGAACAAGCTGAGGAAATCTGCTTCTTTTGGAATCCGAAGCAACGGAAGCAGTTTCCCAACTGCTTCATCCACGATGCCAACACATGTTGATGAACCTGATGTATCATGGGTTCAATCTCTTGTCAAGGATGGACCTCAACCCTTGCAACAACGTGGGCAATTTGGTTTCGAGGATCAGCAGCAGCAGCAGCAGCAGCAGCATAACCCTAACAACGGAGGTCCAGAAATGCTCCCCGCTTGGGTGGAGCAGCTGTACTTTGAGCAGGAGCAGATGGTGGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Ank_3 Ank_4 zf-CCCH

Protein Analysis

708

Amino Acids

77.28

Weight (kDa)

5.62

Isoelectric Point (pI)

53.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	65 - 137	4.8e-08	Ankyrin repeats (3 copies)
zf-CCCH_AtC3H23	PF25512	232 - 265	9.6e-21	AtC3H23-like, CCCH zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011766)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58620
fragaria_vesca	FvH4_3g38750 FvH4_3g38750 FvH4_3g38750
malus_domestica	MD03G1068400.v1.1 MD11G1072800.v1.1
prunus_persica	Prupe.6G056300_v2.0.a1
pyrus_communis	pycom03g05490 pycom11g06050
rosa_chinensis	RchiOBHm_Chr5g0069901
rosa_laevigata	RLG00000036106
rosa_multiflora	Rmu_ssc0000133.1_g000002
rosa_roxburghii	Rroxscaffold_1G00011160
rosa_rugosa	Rorug05G0400600
rosa_samantha	Rh5AG457700 Rh5BG476500 Rh5CG500300 Rh5DG488400
rosa_wichuraiana	Rw5G042740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	4 cut(s)	863, 1227, 1486, 1575
AccI	GTMKAC	1 cut(s)	707
AccII	CGCG	1 cut(s)	1510
AciI	CCGC	5 cut(s)	315, 374, 793, 925, 2076
AclWI	GGATC	5 cut(s)	205, 794, 1311, 1474, 2022
AcyI	GRCGYC	1 cut(s)	227
AdeI	CACNNNGTG	1 cut(s)	1805
AfaI	GTAC	3 cut(s)	182, 923, 2096
AfiI	CCNNNNNNNGG	4 cut(s)	1175, 1300, 2054, 2080
AflIII	ACRYGT	2 cut(s)	264, 1924
AhdI	GACNNNNNGTC	1 cut(s)	576
AjnI	CCWGG	6 cut(s)	775, 1228, 1293, 1425, 1609, 1801
AjuI	GAANNNNNNNTTGG	2 cut(s)	482, 514
Alw21I	GWGCWC	1 cut(s)	421
Alw26I	GTCTC	2 cut(s)	563, 941
AlwI	GGATC	5 cut(s)	205, 794, 1311, 1474, 2022
AlwNI	CAGNNNCTG	2 cut(s)	1025, 1658
AoxI	GGCC	2 cut(s)	1501, 1572
ArsI	GACNNNNNNTTYG	4 cut(s)	123, 155, 1445, 1477
AseI	ATTAAT	1 cut(s)	564
Asp700I	GAANNNNTTC	2 cut(s)	996, 1618
AspA2I	CCTAGG	1 cut(s)	1169
AspLEI	GCGC	1 cut(s)	1554
AspS9I	GGNCC	5 cut(s)	852, 1157, 1573, 1973, 2060
AsuC2I	CCSGG	1 cut(s)	836
AsuHPI	GGTGA	4 cut(s)	458, 902, 1374, 1791
AvaII	GGWCC	4 cut(s)	852, 1157, 1973, 2060
AvrII	CCTAGG	1 cut(s)	1169
BaeGI	GKGCMC	1 cut(s)	764
BanII	GRGCYC	2 cut(s)	129, 1799
BauI	CACGAG	1 cut(s)	606
Bbv12I	GWGCWC	1 cut(s)	421
BbvCI	CCTCAGC	1 cut(s)	1850
BccI	CCATC	5 cut(s)	614, 1117, 1357, 1964, 2107
BcgI	CGANNNNNNTGC	4 cut(s)	453, 487, 1531, 1565
BciT130I	CCWGG	6 cut(s)	777, 1230, 1295, 1427, 1611, 1803
BciVI	GTATCC	1 cut(s)	651
BclI	TGATCA	1 cut(s)	453
BcnI	CCSGG	1 cut(s)	836
BcoDI	GTCTC	2 cut(s)	563, 941
BfaI	CTAG	5 cut(s)	101, 732, 1170, 1260, 1647
BfmI	CTRYAG	2 cut(s)	856, 1568
BfuAI	ACCTGC	4 cut(s)	863, 1227, 1486, 1575
BfuI	GTATCC	1 cut(s)	651
BlnI	CCTAGG	1 cut(s)	1169
BlpI	GCTNAGC	1 cut(s)	1289
Bme1390I	CCNGG	7 cut(s)	777, 836, 1230, 1295, 1427, 1611, 1803
Bme18I	GGWCC	4 cut(s)	852, 1157, 1973, 2060
BmeRI	GACNNNNNGTC	1 cut(s)	576
BmgT120I	GGNCC	5 cut(s)	852, 1157, 1573, 1973, 2060
BmiI	GGNNCC	3 cut(s)	13, 853, 1345
BmrFI	CCNGG	7 cut(s)	777, 836, 1230, 1295, 1427, 1611, 1803
BmrI	ACTGGG	6 cut(s)	519, 913, 1522, 1554, 1575, 1828
BmsI	GCATC	8 cut(s)	60, 415, 944, 1532, 1539, 1630, 1652, 1905
BmuI	ACTGGG	6 cut(s)	519, 913, 1522, 1554, 1575, 1828
BpmI	CTGGAG	3 cut(s)	798, 1316, 1758
Bpu10I	CCTNAGC	1 cut(s)	1850
Bpu1102I	GCTNAGC	1 cut(s)	1289
BpuEI	CTTGAG	1 cut(s)	497
BpuMI	CCSGG	1 cut(s)	836
BsaHI	GRCGYC	1 cut(s)	227
BsaI	GGTCTC	1 cut(s)	941
BsaJI	CCNNGG	7 cut(s)	33, 775, 803, 1040, 1169, 1228, 1426
BsaWI	WCCGGW	1 cut(s)	950
BsaXI	ACNNNNNCTCC	6 cut(s)	1120, 1150, 1687, 1717, 2078, 2108
Bsc4I	CCNNNNNNNGG	4 cut(s)	1175, 1300, 2054, 2080
Bse118I	RCCGGY	1 cut(s)	1721
BseBI	CCWGG	6 cut(s)	777, 1230, 1295, 1427, 1611, 1803
BseDI	CCNNGG	7 cut(s)	33, 775, 803, 1040, 1169, 1228, 1426
BseGI	GGATG	9 cut(s)	75, 949, 959, 1109, 1523, 1554, 1601, 1907, 1975
BseLI	CCNNNNNNNGG	4 cut(s)	1175, 1300, 2054, 2080
BseMII	CTCAG	2 cut(s)	1065, 1841
BseRI	GAGGAG	3 cut(s)	233, 594, 1446
BseSI	GKGCMC	1 cut(s)	764
BsgI	GTGCAG	2 cut(s)	25, 1671
Bsh1236I	CGCG	1 cut(s)	1510
BshFI	GGCC	2 cut(s)	1503, 1574
BsiHKAI	GWGCWC	1 cut(s)	421
BsiSI	CCGG	3 cut(s)	836, 951, 1722
BslFI	GGGAC	3 cut(s)	865, 987, 1563
BslI	CCNNNNNNNGG	4 cut(s)	1175, 1300, 2054, 2080
BsmAI	GTCTC	2 cut(s)	563, 941
BsmFI	GGGAC	3 cut(s)	865, 987, 1563
BsnI	GGCC	2 cut(s)	1503, 1574
Bso31I	GGTCTC	1 cut(s)	941
Bsp1286I	GDGCHC	4 cut(s)	129, 421, 764, 1799
Bsp143I	GATC	9 cut(s)	115, 197, 237, 453, 649, 799, 1303, 1466, 2014
Bsp1720I	GCTNAGC	1 cut(s)	1289
BspACI	CCGC	5 cut(s)	315, 374, 793, 925, 2076
BspANI	GGCC	2 cut(s)	1503, 1574
BspCNI	CTCAG	2 cut(s)	1064, 1842
BspFNI	CGCG	1 cut(s)	1510
BspHI	TCATGA	2 cut(s)	154, 234
BspLI	GGNNCC	3 cut(s)	13, 853, 1345
BspMAI	CTGCAG	2 cut(s)	860, 1572
BspMI	ACCTGC	4 cut(s)	863, 1227, 1486, 1575
BspPI	GGATC	5 cut(s)	205, 794, 1311, 1474, 2022
BspQI	GCTCTTC	3 cut(s)	342, 987, 1830
BspTNI	GGTCTC	1 cut(s)	941
BsrFI	RCCGGY	1 cut(s)	1721
BssAI	RCCGGY	1 cut(s)	1721
BssECI	CCNNGG	7 cut(s)	33, 775, 803, 1040, 1169, 1228, 1426
BssMI	GATC	9 cut(s)	115, 197, 237, 453, 649, 799, 1303, 1466, 2014
BssNAI	GTATAC	1 cut(s)	708
BssNI	GRCGYC	1 cut(s)	227
BssSI	CACGAG	1 cut(s)	606
BssT1I	CCWWGG	2 cut(s)	803, 1169
Bst1107I	GTATAC	1 cut(s)	708
Bst2BI	CACGAG	1 cut(s)	606
Bst2UI	CCWGG	6 cut(s)	777, 1230, 1295, 1427, 1611, 1803
Bst4CI	ACNGT	1 cut(s)	1730
Bst6I	CTCTTC	5 cut(s)	141, 342, 663, 987, 1830
BstACI	GRCGYC	1 cut(s)	227
BstAPI	GCANNNNNTGC	1 cut(s)	1649
BstC8I	GCNNGC	9 cut(s)	109, 172, 313, 396, 509, 860, 1572, 1594, 1723
BstDEI	CTNAG	4 cut(s)	1051, 1253, 1289, 1850
BstDSI	CCRYGG	1 cut(s)	33
BstENI	CCTNNNNNAGG	1 cut(s)	1298
BstF5I	GGATG	9 cut(s)	75, 949, 959, 1109, 1523, 1554, 1601, 1907, 1975
BstFNI	CGCG	1 cut(s)	1510
BstHHI	GCGC	1 cut(s)	1554
BstKTI	GATC	9 cut(s)	118, 200, 240, 456, 652, 802, 1306, 1469, 2017
BstMAI	GTCTC	2 cut(s)	563, 941
BstMBI	GATC	9 cut(s)	115, 197, 237, 453, 649, 799, 1303, 1466, 2014
BstNI	CCWGG	6 cut(s)	777, 1230, 1295, 1427, 1611, 1803
BstNSI	RCATGY	2 cut(s)	511, 1928
BstSCI	CCNGG	7 cut(s)	775, 834, 1228, 1293, 1425, 1609, 1801
BstSFI	CTRYAG	2 cut(s)	856, 1568
BstSLI	GKGCMC	1 cut(s)	764
BstUI	CGCG	1 cut(s)	1510
BstX2I	RGATCY	1 cut(s)	1466
BstXI	CCANNNNNNTGG	2 cut(s)	40, 1356
BstYI	RGATCY	1 cut(s)	1466
BstZ17I	GTATAC	1 cut(s)	708
BsuI	GTATCC	1 cut(s)	651
BsuRI	GGCC	2 cut(s)	1503, 1574
BtgI	CCRYGG	1 cut(s)	33
BtgZI	GCGATG	1 cut(s)	126
BtsCI	GGATG	9 cut(s)	75, 949, 959, 1109, 1523, 1554, 1601, 1907, 1975
BtsI	GCAGTG	2 cut(s)	13, 330
BtsIMutI	CAGTG	5 cut(s)	13, 330, 1063, 1152, 1734
BveI	ACCTGC	4 cut(s)	863, 1227, 1486, 1575
Cac8I	GCNNGC	9 cut(s)	109, 172, 313, 396, 509, 860, 1572, 1594, 1723
CaiI	CAGNNNCTG	2 cut(s)	1025, 1658
CciI	TCATGA	2 cut(s)	154, 234
CfoI	GCGC	1 cut(s)	1554
Cfr10I	RCCGGY	1 cut(s)	1721
Cfr13I	GGNCC	5 cut(s)	852, 1157, 1573, 1973, 2060
CseI	GACGC	2 cut(s)	235, 1368
CsiI	ACCWGGT	1 cut(s)	1801
Csp6I	GTAC	3 cut(s)	181, 922, 2095
CviQI	GTAC	3 cut(s)	181, 922, 2095
DdeI	CTNAG	4 cut(s)	1051, 1253, 1289, 1850
DpnI	GATC	9 cut(s)	117, 199, 239, 455, 651, 801, 1305, 1468, 2016
DpnII	GATC	9 cut(s)	115, 197, 237, 453, 649, 799, 1303, 1466, 2014
DraIII	CACNNNGTG	1 cut(s)	1805
DriI	GACNNNNNGTC	1 cut(s)	576
Eam1104I	CTCTTC	5 cut(s)	141, 342, 663, 987, 1830
Eam1105I	GACNNNNNGTC	1 cut(s)	576
EarI	CTCTTC	5 cut(s)	141, 342, 663, 987, 1830
EciI	GGCGGA	1 cut(s)	389
Eco130I	CCWWGG	2 cut(s)	803, 1169
Eco24I	GRGCYC	2 cut(s)	129, 1799
Eco31I	GGTCTC	1 cut(s)	941
Eco32I	GATATC	2 cut(s)	667, 1338
Eco47I	GGWCC	4 cut(s)	852, 1157, 1973, 2060
EcoNI	CCTNNNNNAGG	1 cut(s)	1298
EcoO109I	RGGNCCY	1 cut(s)	852
EcoRII	CCWGG	6 cut(s)	775, 1228, 1293, 1425, 1609, 1801
EcoRV	GATATC	2 cut(s)	667, 1338
EcoT14I	CCWWGG	2 cut(s)	803, 1169
EcoT22I	ATGCAT	1 cut(s)	1645
EcoT38I	GRGCYC	2 cut(s)	129, 1799
ErhI	CCWWGG	2 cut(s)	803, 1169
FalI	AAGNNNNNCTT	2 cut(s)	1606, 1638
FaqI	GGGAC	3 cut(s)	865, 987, 1563
FauI	CCCGC	1 cut(s)	2083
FauNDI	CATATG	1 cut(s)	466
FbaI	TGATCA	1 cut(s)	453
FblI	GTMKAC	1 cut(s)	707
FokI	GGATG	9 cut(s)	82, 956, 966, 1096, 1510, 1561, 1588, 1894, 1982
FriOI	GRGCYC	2 cut(s)	129, 1799
FspBI	CTAG	5 cut(s)	101, 732, 1170, 1260, 1647
GlaI	GCGC	1 cut(s)	1553
GsuI	CTGGAG	3 cut(s)	798, 1316, 1758
HaeIII	GGCC	2 cut(s)	1503, 1574
HapII	CCGG	3 cut(s)	836, 951, 1722
HgaI	GACGC	2 cut(s)	235, 1368
HhaI	GCGC	1 cut(s)	1554
Hin1I	GRCGYC	1 cut(s)	227
Hin6I	GCGC	1 cut(s)	1552
HinP1I	GCGC	1 cut(s)	1552
HindIII	AAGCTT	1 cut(s)	495
HinfI	GANTC	7 cut(s)	410, 1120, 1147, 1402, 1514, 1696, 1872
HpaII	CCGG	3 cut(s)	836, 951, 1722
HphI	GGTGA	4 cut(s)	458, 902, 1374, 1791
Hpy166II	GTNNAC	3 cut(s)	708, 772, 1438
Hpy188I	TCNGA	8 cut(s)	322, 553, 845, 1041, 1542, 1701, 1789, 1877
Hpy188III	TCNNGA	9 cut(s)	119, 155, 235, 422, 581, 662, 670, 1070, 2063
Hpy8I	GTNNAC	3 cut(s)	708, 772, 1438
HpyCH4III	ACNGT	1 cut(s)	1730
HpyCH4IV	ACGT	2 cut(s)	264, 1993
HpyF3I	CTNAG	4 cut(s)	1051, 1253, 1289, 1850
HpySE526I	ACGT	2 cut(s)	264, 1993
Hsp92I	GRCGYC	1 cut(s)	227
HspAI	GCGC	1 cut(s)	1552
KroI	GCCGGC	1 cut(s)	1721
KroNI	GCCGGC	1 cut(s)	1723
Ksp22I	TGATCA	1 cut(s)	453
Kzo9I	GATC	9 cut(s)	115, 197, 237, 453, 649, 799, 1303, 1466, 2014
LguI	GCTCTTC	3 cut(s)	342, 987, 1830
LmnI	GCTCC	6 cut(s)	49, 553, 1230, 2076, 2086, 2107
LweI	GCATC	8 cut(s)	60, 415, 944, 1532, 1539, 1630, 1652, 1905
MabI	ACCWGGT	1 cut(s)	1801
MaeI	CTAG	5 cut(s)	101, 732, 1170, 1260, 1647
MaeII	ACGT	2 cut(s)	264, 1993
MaeIII	GTNAC	5 cut(s)	446, 1380, 1530, 1583, 1730
MalI	GATC	9 cut(s)	117, 199, 239, 455, 651, 801, 1305, 1468, 2016
MboI	GATC	9 cut(s)	115, 197, 237, 453, 649, 799, 1303, 1466, 2014
MfeI	CAATTG	1 cut(s)	284
MflI	RGATCY	1 cut(s)	1466
MhlI	GDGCHC	4 cut(s)	129, 421, 764, 1799
MlyI	GAGTC	2 cut(s)	1141, 1705
MmeI	TCCRAC	1 cut(s)	1565
Mph1103I	ATGCAT	1 cut(s)	1645
MroNI	GCCGGC	1 cut(s)	1721
MroXI	GAANNNNTTC	2 cut(s)	996, 1618
MseI	TTAA	7 cut(s)	297, 303, 564, 720, 1497, 1769, 2122
MslI	CAYNNNNRTG	3 cut(s)	38, 1283, 1929
MspA1I	CMGCKG	4 cut(s)	822, 1655, 1679, 2092
MspI	CCGG	3 cut(s)	836, 951, 1722
MspR9I	CCNGG	7 cut(s)	777, 836, 1230, 1295, 1427, 1611, 1803
MunI	CAATTG	1 cut(s)	284
MvaI	CCWGG	6 cut(s)	777, 1230, 1295, 1427, 1611, 1803
MvnI	CGCG	1 cut(s)	1510
NaeI	GCCGGC	1 cut(s)	1723
NciI	CCSGG	1 cut(s)	836
NdeI	CATATG	1 cut(s)	466
NdeII	GATC	9 cut(s)	115, 197, 237, 453, 649, 799, 1303, 1466, 2014
NgoMIV	GCCGGC	1 cut(s)	1721
NlaIV	GGNNCC	3 cut(s)	13, 853, 1345
NmuCI	GTSAC	2 cut(s)	446, 1380
NsiI	ATGCAT	1 cut(s)	1645
NspI	RCATGY	2 cut(s)	511, 1928
PaeI	GCATGC	1 cut(s)	511
PagI	TCATGA	2 cut(s)	154, 234
PciI	ACATGT	1 cut(s)	1924
PciSI	GCTCTTC	3 cut(s)	342, 987, 1830
PdiI	GCCGGC	1 cut(s)	1723
PdmI	GAANNNNTTC	2 cut(s)	996, 1618
PfeI	GAWTC	5 cut(s)	410, 1120, 1402, 1514, 1872
PfoI	TCCNGGA	1 cut(s)	1609
PleI	GAGTC	2 cut(s)	1141, 1704
PpsI	GAGTC	2 cut(s)	1141, 1704
PpuMI	RGGWCCY	1 cut(s)	852
PscI	ACATGT	1 cut(s)	1924
PshBI	ATTAAT	1 cut(s)	564
Psp5II	RGGWCCY	1 cut(s)	852
Psp6I	CCWGG	6 cut(s)	775, 1228, 1293, 1425, 1609, 1801
PspGI	CCWGG	6 cut(s)	775, 1228, 1293, 1425, 1609, 1801
PspN4I	GGNNCC	3 cut(s)	13, 853, 1345
PspPI	GGNCC	5 cut(s)	852, 1157, 1573, 1973, 2060
PspPPI	RGGWCCY	1 cut(s)	852
PsrI	GAACNNNNNNTAC	2 cut(s)	1935, 1967
PstI	CTGCAG	2 cut(s)	860, 1572
PstNI	CAGNNNCTG	2 cut(s)	1025, 1658
PsuI	RGATCY	1 cut(s)	1466
PvuII	CAGCTG	4 cut(s)	822, 1655, 1679, 2092
RsaI	GTAC	3 cut(s)	182, 923, 2096
RsaNI	GTAC	3 cut(s)	181, 922, 2095
RseI	CAYNNNNRTG	3 cut(s)	38, 1283, 1929
SapI	GCTCTTC	3 cut(s)	342, 987, 1830
SaqAI	TTAA	7 cut(s)	297, 303, 564, 720, 1497, 1769, 2122
Sau3AI	GATC	9 cut(s)	115, 197, 237, 453, 649, 799, 1303, 1466, 2014
Sau96I	GGNCC	5 cut(s)	852, 1157, 1573, 1973, 2060
SbfI	CCTGCAGG	2 cut(s)	860, 1572
SchI	GAGTC	2 cut(s)	1141, 1705
ScrFI	CCNGG	7 cut(s)	777, 836, 1230, 1295, 1427, 1611, 1803
SdaI	CCTGCAGG	2 cut(s)	860, 1572
SduI	GDGCHC	4 cut(s)	129, 421, 764, 1799
SexAI	ACCWGGT	1 cut(s)	1801
SfaNI	GCATC	8 cut(s)	60, 415, 944, 1532, 1539, 1630, 1652, 1905
SfcI	CTRYAG	2 cut(s)	856, 1568
SinI	GGWCC	4 cut(s)	852, 1157, 1973, 2060
SmiMI	CAYNNNNRTG	3 cut(s)	38, 1283, 1929
SmlI	CTYRAG	1 cut(s)	512
SmoI	CTYRAG	1 cut(s)	512
SphI	GCATGC	1 cut(s)	511
Sse8387I	CCTGCAGG	2 cut(s)	860, 1572
SsiI	CCGC	5 cut(s)	315, 374, 793, 925, 2076
SspMI	CTAG	5 cut(s)	101, 732, 1170, 1260, 1647
StyD4I	CCNGG	7 cut(s)	775, 834, 1228, 1293, 1425, 1609, 1801
StyI	CCWWGG	2 cut(s)	803, 1169
TaaI	ACNGT	1 cut(s)	1730
TaiI	ACGT	2 cut(s)	267, 1996
TaqI	TCGA	8 cut(s)	86, 141, 200, 413, 473, 1016, 1405, 2010
TatI	WGTACW	1 cut(s)	2094
TauI	GCSGC	1 cut(s)	796
TfiI	GAWTC	5 cut(s)	410, 1120, 1402, 1514, 1872
Tru1I	TTAA	7 cut(s)	297, 303, 564, 720, 1497, 1769, 2122
Tru9I	TTAA	7 cut(s)	297, 303, 564, 720, 1497, 1769, 2122
TscAI	CASTG	5 cut(s)	13, 337, 1063, 1159, 1741
TseFI	GTSAC	2 cut(s)	446, 1380
Tsp45I	GTSAC	2 cut(s)	446, 1380
TspDTI	ATGAA	7 cut(s)	329, 549, 1171, 1577, 1696, 1896, 1947
TspGWI	ACGGA	4 cut(s)	50, 641, 1899, 2070
TspRI	CASTG	5 cut(s)	13, 337, 1063, 1159, 1741
VpaK11BI	GGWCC	4 cut(s)	852, 1157, 1973, 2060
VspI	ATTAAT	1 cut(s)	564
XagI	CCTNNNNNAGG	1 cut(s)	1298
XceI	RCATGY	2 cut(s)	511, 1928
XmaJI	CCTAGG	1 cut(s)	1169
XmiI	GTMKAC	1 cut(s)	707
XmnI	GAANNNNTTC	2 cut(s)	996, 1618
XspI	CTAG	5 cut(s)	101, 732, 1170, 1260, 1647
Zsp2I	ATGCAT	1 cut(s)	1645

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.