Rmu_ssc0000133.1_g000002

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000133.1
OG0011766
Physical Location & Seq
Reverse (-)
5919 .. 8009
2091 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000133.1_g000002.1.cds

Sequence Viewer

Length: 2091 bp
atgtgcagcggttcaaagaggaaaccttgttccactgactcgaccatggatcatgagaaacaggaagggttgaggtttagcttttcgattttgttggagttagcagcggccgatgatcttgatgggttcaaaagtgctgtggaggaagatggtcatgatgttgatgaggtgagttattggtatgggaggcgtgttggctccaagaagatggggtttgaggagagaacaccgcttatgattgctgctatgtttggcagcatgaatgtgttgaattatatcctccaaacgtgtcgcgttgatgttaatagagcttgtggttcagatagagccacggctcttcactgtgctgctgctggtggctctggtgctgcagctgaggttgtcaaggtcttgcttgctgcttctgcggatgccagttctattgatgttaatggaaaccaagcaggggacttgatcgcacctgcctacagttcagccttcaattcgaggaagaaagctttggaagtcatgctcaagggcgattcgggttttgatgaagcttttgatttctctgagcagatggctagggaagcagaaggtgaagatcagcaagagatcacaactcctcgtgcctcaaaggatgggacggagaggaaggaatatcctgttgatctttcccttcctgacatcaagaacgggatttatagcacagatgagtttagaatgtatactttcaaggtcaagccttgctctagggcttactcacacgattggacagagtgcccatttgtacacccaggggaaaatgcaaggcggcgtgatccaaggaaatatcattatagctgcgttccttgcccagagttcagaaaggggacttgcaggcaaggggatgcttgtgaatatgcacatggtatttttgaatgttggcttcaccctgcccagtaccgcacccgtctttgcaaggatgagactgggtgcacaagaagggtctgtttctttgctcacaagacagaagagcttcgtcccctctatgcttcaacaggttcggctgtgccttctcccagatcattctctgcgggtgcttctctggacatgggatcattgagtccactttccctcaactctccgtctgccctgattccttctgtttcaactccacccatgactccaactggtgcttcttcccctatgaatggaaacatgtggcaaaatcagccaaatattgcgccccccaccttgcagctcccaggtagcaggttgaagtctactctaagtgctagagatatggatttagacatggaattccttagcctggaaaatcatcgtcgcaggcagcgcttgatagatgagttgtccagtccctcatccggctggaataatggcttatctagtccttcagccttcgcggcctctgggaatcgaataggggaattgaataggattggaggagtgaggcccactaacctcgaagatgcttttggatcaattgatcctgcaatgttgcctcagttacaggggctttcacgggataccacagcatcccagttgcaatctccaactggtatccagatgcgccaaaacatgaacatgcaactccgatcaagctactcaagcagccctgcttcatctcctgttaggggttctcctttgcatgggtttgatgcatctggtgcagcagcagttttgagttccaggtcagctgcatttgcaaagcagcggagccagagcttcattgagcgcagtgcaaaccacaattatgggttttcctcacccgctgattctggaactccaaaaacctccaacctttcaaattggggctctcctagtggaaagctagactggggtatccagggggaagaactgaataagctgaggaaatctgcttcttttggataccgaaacaatggaaacagcgttccaactgttgatgagcctgatgtatcttgggtaaacacccttgttaaggatgcaccaggaccttcgagacctggaccacttggttttgaagaccagcagcagcagcagcagtgtcacctcgacaatggagattcagagatactccctgcttgggtggagcagatgtacttagagcaggagcagatggtggcttaa

Protein Analysis

696

Amino Acids

75.89

Weight (kDa)

5.67

Isoelectric Point (pI)

56.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011766)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58620
fragaria_vesca FvH4_3g38750 FvH4_3g38750 FvH4_3g38750
malus_domestica MD03G1068400.v1.1 MD11G1072800.v1.1
prunus_persica Prupe.6G056300_v2.0.a1
pyrus_communis pycom03g05490 pycom11g06050
rosa_chinensis RchiOBHm_Chr5g0069901
rosa_laevigata RLG00000036106
rosa_multiflora Rmu_ssc0000133.1_g000002
rosa_roxburghii Rroxscaffold_1G00011160
rosa_rugosa Rorug05G0400600
rosa_samantha Rh5AG457700 Rh5BG476500 Rh5CG500300 Rh5DG488400
rosa_wichuraiana Rw5G042740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 469
Acc36I ACCTGC 2 cut(s) 469, 1224
AccI GTMKAC 2 cut(s) 707, 1244
AccII CGCG 2 cut(s) 294, 1385
AclWI GGATC 5 cut(s) 57, 794, 1084, 1463, 1468
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 1 cut(s) 1280
AcuI CTGAAG 1 cut(s) 1359
AfaI GTAC 3 cut(s) 771, 923, 2063
AfeI AGCGCT 1 cut(s) 1316
AfiI CCNNNNNNNGG 8 cut(s) 445, 1172, 1291, 1346, 1616, 1631, 1942, 2047
AflIII ACRYGT 2 cut(s) 287, 1179
AjnI CCWGG 7 cut(s) 775, 1225, 1290, 1670, 1827, 1951, 1966
AjuI GAANNNNNNNTTGG 6 cut(s) 482, 514, 1141, 1173, 1440, 1472
AloI GAACNNNNNNTCC 4 cut(s) 13, 45, 1878, 1910
Alw21I GWGCWC 1 cut(s) 959
Alw26I GTCTC 2 cut(s) 941, 1957
Alw44I GTGCAC 1 cut(s) 955
AlwI GGATC 5 cut(s) 57, 794, 1084, 1463, 1468
Aor51HI AGCGCT 1 cut(s) 1316
AoxI GGCC 3 cut(s) 108, 1386, 1433
ApaLI GTGCAC 1 cut(s) 955
ApoI RAATTY 1 cut(s) 1280
Asp700I GAANNNNTTC 1 cut(s) 996
AspLEI GCGC 4 cut(s) 1207, 1317, 1554, 1719
AspS9I GGNCC 3 cut(s) 1434, 1955, 1970
AsuHPI GGTGA 5 cut(s) 181, 590, 902, 1740, 2003
AvaII GGWCC 2 cut(s) 1955, 1970
BaeGI GKGCMC 2 cut(s) 764, 959
BaeI ACNNNNGTAYC 2 cut(s) 1807, 1840
BanII GRGCYC 1 cut(s) 1799
BauI CACGAG 1 cut(s) 606
BbsI GAAGAC 1 cut(s) 1992
Bbv12I GWGCWC 1 cut(s) 959
BbvCI CCTCAGC 2 cut(s) 375, 1850
BccI CCATC 6 cut(s) 116, 143, 202, 553, 614, 2074
BceAI ACGGC 1 cut(s) 348
BciT130I CCWGG 7 cut(s) 777, 1227, 1292, 1672, 1829, 1953, 1968
BciVI GTATCC 4 cut(s) 1501, 1553, 1835, 1865
BcoDI GTCTC 2 cut(s) 941, 1957
BfaI CTAG 6 cut(s) 564, 732, 1257, 1368, 1803, 1814
BfmI CTRYAG 2 cut(s) 369, 466
BfoI RGCGCY 1 cut(s) 1318
BfuAI ACCTGC 2 cut(s) 469, 1224
BfuI GTATCC 4 cut(s) 1501, 1553, 1835, 1865
BglI GCCNNNNNGGC 1 cut(s) 1385
Bme1390I CCNGG 7 cut(s) 777, 1227, 1292, 1672, 1829, 1953, 1968
Bme18I GGWCC 2 cut(s) 1955, 1970
BmgT120I GGNCC 3 cut(s) 1434, 1955, 1970
BmiI GGNNCC 2 cut(s) 199, 1700
BmrFI CCNGG 7 cut(s) 777, 1227, 1292, 1672, 1829, 1953, 1968
BmrI ACTGGG 4 cut(s) 913, 960, 1516, 1828
BmsI GCATC 8 cut(s) 400, 859, 1441, 1526, 1539, 1630, 1652, 1936
BmuI ACTGGG 4 cut(s) 913, 960, 1516, 1828
BpiI GAAGAC 1 cut(s) 1992
Bpu10I CCTNAGC 3 cut(s) 375, 1286, 1850
BpuEI CTTGAG 2 cut(s) 497, 1573
BsaI GGTCTC 1 cut(s) 1957
BsaJI CCNNGG 7 cut(s) 45, 330, 775, 776, 803, 1225, 1828
BsaXI ACNNNNNCTCC 2 cut(s) 2045, 2075
Bsc4I CCNNNNNNNGG 8 cut(s) 445, 1172, 1291, 1346, 1616, 1631, 1942, 2047
Bse1I ACTGG 8 cut(s) 414, 919, 955, 1156, 1335, 1522, 1543, 1823
Bse3DI GCAATG 1 cut(s) 1482
BseBI CCWGG 7 cut(s) 777, 1227, 1292, 1672, 1829, 1953, 1968
BseDI CCNNGG 7 cut(s) 45, 330, 775, 776, 803, 1225, 1828
BseGI GGATG 7 cut(s) 415, 625, 874, 949, 1343, 1517, 1951
BseLI CCNNNNNNNGG 8 cut(s) 445, 1172, 1291, 1346, 1616, 1631, 1942, 2047
BseMI GCAATG 1 cut(s) 1482
BseMII CTCAG 4 cut(s) 366, 543, 1499, 1841
BseNI ACTGG 8 cut(s) 414, 919, 955, 1156, 1335, 1522, 1543, 1823
BseRI GAGGAG 3 cut(s) 233, 594, 1440
BseSI GKGCMC 2 cut(s) 764, 959
BseX3I CGGCCG 1 cut(s) 108
BsgI GTGCAG 2 cut(s) 25, 1671
Bsh1236I CGCG 2 cut(s) 294, 1385
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 3 cut(s) 110, 1388, 1435
BsiEI CGRYCG 1 cut(s) 111
BsiHKAI GWGCWC 1 cut(s) 959
BsiSI CCGG 1 cut(s) 1347
BslFI GGGAC 5 cut(s) 461, 637, 865, 987, 1323
BslI CCNNNNNNNGG 8 cut(s) 445, 1172, 1291, 1346, 1616, 1631, 1942, 2047
BsmAI GTCTC 2 cut(s) 941, 1957
BsmFI GGGAC 5 cut(s) 461, 637, 865, 987, 1323
BsnI GGCC 3 cut(s) 110, 1388, 1435
Bso31I GGTCTC 1 cut(s) 1957
Bsp1286I GDGCHC 3 cut(s) 764, 959, 1799
Bsp1407I TGTACA 1 cut(s) 769
Bsp19I CCATGG 1 cut(s) 45
BspANI GGCC 3 cut(s) 110, 1388, 1435
BspCNI CTCAG 4 cut(s) 367, 544, 1498, 1842
BspFNI CGCG 2 cut(s) 294, 1385
BspHI TCATGA 2 cut(s) 52, 154
BspLI GGNNCC 2 cut(s) 199, 1700
BspMAI CTGCAG 1 cut(s) 373
BspMI ACCTGC 2 cut(s) 469, 1224
BspPI GGATC 5 cut(s) 57, 794, 1084, 1463, 1468
BspQI GCTCTTC 2 cut(s) 342, 987
BspTNI GGTCTC 1 cut(s) 1957
BsrDI GCAATG 1 cut(s) 1482
BsrGI TGTACA 1 cut(s) 769
BsrI ACTGG 8 cut(s) 414, 919, 955, 1156, 1335, 1522, 1543, 1823
BssECI CCNNGG 7 cut(s) 45, 330, 775, 776, 803, 1225, 1828
BssNAI GTATAC 1 cut(s) 708
BssSI CACGAG 1 cut(s) 606
BssT1I CCWWGG 2 cut(s) 45, 803
Bst1107I GTATAC 1 cut(s) 708
Bst2BI CACGAG 1 cut(s) 606
Bst2UI CCWGG 7 cut(s) 777, 1227, 1292, 1672, 1829, 1953, 1968
Bst4CI ACNGT 3 cut(s) 344, 470, 1903
Bst6I CTCTTC 2 cut(s) 342, 987
BstAPI GCANNNNNTGC 1 cut(s) 1649
BstAUI TGTACA 1 cut(s) 769
BstC8I GCNNGC 3 cut(s) 396, 860, 1310
BstDEI CTNAG 7 cut(s) 375, 552, 1250, 1286, 1485, 1850, 2065
BstDSI CCRYGG 2 cut(s) 45, 330
BstENI CCTNNNNNAGG 1 cut(s) 1940
BstF5I GGATG 7 cut(s) 415, 625, 874, 949, 1343, 1517, 1951
BstFNI CGCG 2 cut(s) 294, 1385
BstH2I RGCGCY 1 cut(s) 1318
BstHHI GCGC 4 cut(s) 1207, 1317, 1554, 1719
BstMAI GTCTC 2 cut(s) 941, 1957
BstMCI CGRYCG 1 cut(s) 111
BstNI CCWGG 7 cut(s) 777, 1227, 1292, 1672, 1829, 1953, 1968
BstNSI RCATGY 2 cut(s) 1183, 1570
BstSCI CCNGG 7 cut(s) 775, 1225, 1290, 1670, 1827, 1951, 1966
BstSFI CTRYAG 2 cut(s) 369, 466
BstSLI GKGCMC 2 cut(s) 764, 959
BstUI CGCG 2 cut(s) 294, 1385
BstV2I GAAGAC 1 cut(s) 1992
BstXI CCANNNNNNTGG 2 cut(s) 208, 1736
BstZ17I GTATAC 1 cut(s) 708
BstZI CGGCCG 1 cut(s) 108
BsuI GTATCC 4 cut(s) 1501, 1553, 1835, 1865
BsuRI GGCC 3 cut(s) 110, 1388, 1435
BtgI CCRYGG 2 cut(s) 45, 330
BtsCI GGATG 7 cut(s) 415, 625, 874, 949, 1343, 1517, 1951
BtsI GCAGTG 2 cut(s) 1726, 2012
BtsIMutI CAGTG 4 cut(s) 33, 340, 1726, 2012
BveI ACCTGC 2 cut(s) 469, 1224
Cac8I GCNNGC 3 cut(s) 396, 860, 1310
CciI TCATGA 2 cut(s) 52, 154
CfoI GCGC 4 cut(s) 1207, 1317, 1554, 1719
Cfr13I GGNCC 3 cut(s) 1434, 1955, 1970
Csp6I GTAC 3 cut(s) 770, 922, 2062
CviQI GTAC 3 cut(s) 770, 922, 2062
DdeI CTNAG 7 cut(s) 375, 552, 1250, 1286, 1485, 1850, 2065
EaeI YGGCCR 1 cut(s) 108
EagI CGGCCG 1 cut(s) 108
Eam1104I CTCTTC 2 cut(s) 342, 987
EarI CTCTTC 2 cut(s) 342, 987
EclXI CGGCCG 1 cut(s) 108
Eco130I CCWWGG 2 cut(s) 45, 803
Eco24I GRGCYC 1 cut(s) 1799
Eco31I GGTCTC 1 cut(s) 1957
Eco47I GGWCC 2 cut(s) 1955, 1970
Eco47III AGCGCT 1 cut(s) 1316
Eco52I CGGCCG 1 cut(s) 108
Eco57I CTGAAG 1 cut(s) 1359
EcoNI CCTNNNNNAGG 1 cut(s) 1940
EcoO109I RGGNCCY 1 cut(s) 1955
EcoRI GAATTC 1 cut(s) 1280
EcoRII CCWGG 7 cut(s) 775, 1225, 1290, 1670, 1827, 1951, 1966
EcoT14I CCWWGG 2 cut(s) 45, 803
EcoT22I ATGCAT 1 cut(s) 1645
EcoT38I GRGCYC 1 cut(s) 1799
ErhI CCWWGG 2 cut(s) 45, 803
FaqI GGGAC 5 cut(s) 461, 637, 865, 987, 1323
FauI CCCGC 2 cut(s) 1048, 1759
FblI GTMKAC 2 cut(s) 707, 1244
FokI GGATG 7 cut(s) 422, 632, 881, 956, 1330, 1504, 1958
FriOI GRGCYC 1 cut(s) 1799
FspBI CTAG 6 cut(s) 564, 732, 1257, 1368, 1803, 1814
GlaI GCGC 4 cut(s) 1206, 1316, 1553, 1718
HaeII RGCGCY 1 cut(s) 1318
HaeIII GGCC 3 cut(s) 110, 1388, 1435
HapII CCGG 1 cut(s) 1347
HhaI GCGC 4 cut(s) 1207, 1317, 1554, 1719
Hin6I GCGC 4 cut(s) 1205, 1315, 1552, 1717
HinP1I GCGC 4 cut(s) 1205, 1315, 1552, 1717
HindIII AAGCTT 2 cut(s) 495, 537
HinfI GANTC 8 cut(s) 38, 521, 1084, 1117, 1144, 1396, 1757, 2027
HpaII CCGG 1 cut(s) 1347
HphI GGTGA 5 cut(s) 181, 590, 902, 1740, 2003
Hpy166II GTNNAC 6 cut(s) 708, 772, 957, 1088, 1245, 1930
Hpy188I TCNGA 5 cut(s) 322, 553, 845, 1577, 2032
Hpy188III TCNNGA 9 cut(s) 53, 119, 155, 662, 670, 1067, 1546, 1761, 1962
Hpy8I GTNNAC 6 cut(s) 708, 772, 957, 1088, 1245, 1930
Hpy99I CGWCG 1 cut(s) 1308
HpyCH4III ACNGT 3 cut(s) 344, 470, 1903
HpyCH4IV ACGT 1 cut(s) 287
HpyF3I CTNAG 7 cut(s) 375, 552, 1250, 1286, 1485, 1850, 2065
HpySE526I ACGT 1 cut(s) 287
HspAI GCGC 4 cut(s) 1205, 1315, 1552, 1717
LguI GCTCTTC 2 cut(s) 342, 987
LmnI GCTCC 5 cut(s) 203, 1227, 1698, 2053, 2074
LweI GCATC 8 cut(s) 400, 859, 1441, 1526, 1539, 1630, 1652, 1936
MaeI CTAG 6 cut(s) 564, 732, 1257, 1368, 1803, 1814
MaeII ACGT 1 cut(s) 287
MaeIII GTNAC 2 cut(s) 1488, 2009
MfeI CAATTG 1 cut(s) 1464
MhlI GDGCHC 3 cut(s) 764, 959, 1799
MluCI AATT 7 cut(s) 271, 481, 1280, 1409, 1464, 1732, 1789
MlyI GAGTC 3 cut(s) 32, 1093, 1138
MmeI TCCRAC 5 cut(s) 75, 1172, 1559, 1803, 1922
Mph1103I ATGCAT 1 cut(s) 1645
MroXI GAANNNNTTC 1 cut(s) 996
MseI TTAA 4 cut(s) 303, 429, 1941, 2089
MslI CAYNNNNRTG 3 cut(s) 263, 1565, 1734
MspA1I CMGCKG 6 cut(s) 9, 107, 374, 1679, 1696, 1754
MspI CCGG 1 cut(s) 1347
MspR9I CCNGG 7 cut(s) 777, 1227, 1292, 1672, 1829, 1953, 1968
MunI CAATTG 1 cut(s) 1464
MvaI CCWGG 7 cut(s) 777, 1227, 1292, 1672, 1829, 1953, 1968
MvnI CGCG 2 cut(s) 294, 1385
NcoI CCATGG 1 cut(s) 45
NlaIV GGNNCC 2 cut(s) 199, 1700
NmuCI GTSAC 1 cut(s) 2009
NsiI ATGCAT 1 cut(s) 1645
NspI RCATGY 2 cut(s) 1183, 1570
PagI TCATGA 2 cut(s) 52, 154
PaqCI CACCTGC 1 cut(s) 469
PasI CCCWGGG 1 cut(s) 776
PciI ACATGT 1 cut(s) 1179
PciSI GCTCTTC 2 cut(s) 342, 987
PdmI GAANNNNTTC 1 cut(s) 996
PfeI GAWTC 5 cut(s) 521, 1117, 1396, 1757, 2027
PleI GAGTC 3 cut(s) 32, 1092, 1138
PpsI GAGTC 3 cut(s) 32, 1092, 1138
PpuMI RGGWCCY 1 cut(s) 1955
PscI ACATGT 1 cut(s) 1179
Psp5II RGGWCCY 1 cut(s) 1955
Psp6I CCWGG 7 cut(s) 775, 1225, 1290, 1670, 1827, 1951, 1966
PspGI CCWGG 7 cut(s) 775, 1225, 1290, 1670, 1827, 1951, 1966
PspN4I GGNNCC 2 cut(s) 199, 1700
PspPI GGNCC 3 cut(s) 1434, 1955, 1970
PspPPI RGGWCCY 1 cut(s) 1955
PstI CTGCAG 1 cut(s) 373
PvuII CAGCTG 2 cut(s) 374, 1679
RsaI GTAC 3 cut(s) 771, 923, 2063
RsaNI GTAC 3 cut(s) 770, 922, 2062
RseI CAYNNNNRTG 3 cut(s) 263, 1565, 1734
SapI GCTCTTC 2 cut(s) 342, 987
SaqAI TTAA 4 cut(s) 303, 429, 1941, 2089
Sau96I GGNCC 3 cut(s) 1434, 1955, 1970
SchI GAGTC 3 cut(s) 32, 1093, 1138
ScrFI CCNGG 7 cut(s) 777, 1227, 1292, 1672, 1829, 1953, 1968
SduI GDGCHC 3 cut(s) 764, 959, 1799
SfaNI GCATC 8 cut(s) 400, 859, 1441, 1526, 1539, 1630, 1652, 1936
SfcI CTRYAG 2 cut(s) 369, 466
SinI GGWCC 2 cut(s) 1955, 1970
SmiMI CAYNNNNRTG 3 cut(s) 263, 1565, 1734
SmlI CTYRAG 2 cut(s) 512, 1588
SmoI CTYRAG 2 cut(s) 512, 1588
Sse9I AATT 7 cut(s) 271, 481, 1280, 1409, 1464, 1732, 1789
SspI AATATT 1 cut(s) 1201
SspMI CTAG 6 cut(s) 564, 732, 1257, 1368, 1803, 1814
StyD4I CCNGG 7 cut(s) 775, 1225, 1290, 1670, 1827, 1951, 1966
StyI CCWWGG 2 cut(s) 45, 803
TaaI ACNGT 3 cut(s) 344, 470, 1903
TaiI ACGT 1 cut(s) 290
TaqI TCGA 7 cut(s) 41, 86, 485, 1399, 1446, 1961, 2016
TasI AATT 7 cut(s) 271, 481, 1280, 1409, 1464, 1732, 1789
TatI WGTACW 2 cut(s) 769, 2061
TauI GCSGC 3 cut(s) 110, 796, 1388
TfiI GAWTC 5 cut(s) 521, 1117, 1396, 1757, 2027
Tru1I TTAA 4 cut(s) 303, 429, 1941, 2089
Tru9I TTAA 4 cut(s) 303, 429, 1941, 2089
TscAI CASTG 4 cut(s) 40, 347, 1726, 2012
TseFI GTSAC 1 cut(s) 2009
Tsp45I GTSAC 1 cut(s) 2009
TspDTI ATGAA 6 cut(s) 275, 549, 1184, 1577, 1593, 1699
TspGWI ACGGA 2 cut(s) 641, 1095
TspRI CASTG 4 cut(s) 40, 347, 1726, 2012
VneI GTGCAC 1 cut(s) 955
VpaK11BI GGWCC 2 cut(s) 1955, 1970
XagI CCTNNNNNAGG 1 cut(s) 1940
XapI RAATTY 1 cut(s) 1280
XceI RCATGY 2 cut(s) 1183, 1570
XmiI GTMKAC 2 cut(s) 707, 1244
XmnI GAANNNNTTC 1 cut(s) 996
XspI CTAG 6 cut(s) 564, 732, 1257, 1368, 1803, 1814
Zsp2I ATGCAT 1 cut(s) 1645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.