Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0007595

Physical Location & Seq

Genomic Coordinates Reverse (-)

6527538 .. 6531654

Gene Structure 4117 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1081000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1470 bp

ATGGGAGCTCGTTGCTCCAAGTTTTCCCTCTGCTTCTGGCCGTCCAATACCAAATCCAATCCCAACGACTCTTCAGACCCAGAGAATAAGAACAAAAATGAGGATTCGCTTCCTGGGTTCAACGAGTTCAGCCTGGACCAACTGAAAGCTGCCACGTCAGGATTCTCGTCGGAGAACGTGGTCTCCGAGCACGGAGAGAAAGCTCCCAATGTGGTCTACAAAGGGAAGCTCGAAGACGGCCGTTGGATCGCTGTTAAGCGCTTCAACCGATCAGCTTGGCCTGATTCGCGCCAATTTCTGGAGGAAGCTAGAGCTGTGGGGCAGCTGAGGAACGAGCGGTTGGCGAATTTGATTGGGTGTTGCTGTGAGGGTGACAATAGATTGCTTGTGGCCGAGTTCATGCCTAATGAAACTCTCTCTAAGCATCTCTTTCATTGGGAAGCTCAGCCGATGAAATGGGCGATGAGGTTGCGAGTGGCTTTATATCTCGCACAAGCTTTAGACTATTGCAGCAGTAAAGGGCAGGCATTGTACCATGATCTTAATGCTTATAGAGTTCTCTTTGATCAGGATGGGAATCCCAGACTCTCCTGCTTTGGTCTTATGAAGAATAGCAAAGACGGCAAGAGCTATAGTACCAACCTGGCTTTCACCCCTCCAGAGTACTTGAGGACTGGAAGAGTGATACCAGAAAGTTTAGTTTACAGCTTTGGGACCGTATTGCTTGATCTGCTCAGTGGAAAACATATTCCCCCAAGCCATGCACTTGACCTGATACGTGGCAAAAACTTTTTGATGCTGATGGACTCATGCTTGGAAGGTCATTTCTCAAATGATGATGGAACTGAGCTGGTGAGGTTAGCTTCACGATGCTTGCAGTACGAACCTCGTGAGAGGCCAAATGCCAAGTCTCTTGTGACTGCCCTCATTCCTCTTCAAAAAGAAACTGAGGTTCCATCGTACGTTTTATTGGGTATTGCACATGGAAATACGCTTCTGAATCAGACATCGTTGTCACCTCTAGGTGAAGCTTGCTCGAGATTGGATCTTACTGCAATACATGAAATATTGGAGAAGCTTGGATACAAGGATGATGAGGGGGTCACAAATGATCTTTCTTTCCAAATGTGGACAAATCAAATACAAGAGACATTGAACTCTAAAAAGCATGCAGATGCTGCATTTCGAGCGAAAGATTTTGCTGCTGCCATAGATTCATACACAGAATTCATTGACGGTGGAACAATGATATCTCCAACTGTGTTTGCTCGACGTTGCTTATGTTACTTGATGAATGACATGGCGCAAGAAGCTCTTGGAGATGCAATGCAAGCACTAGTAATAAACCCCGAATGGTCGACTGGATTCTATCTTCAAGCGGCTGCCCTCAAGAGCCTGGGGATGGACAACGATGCGCAGGAAACGCTAAAAGACGGATCATCCTTGGAAGCCAAAAGAGAGAAAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009506 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009741 GO:0009742 GO:0009755 GO:0009987 GO:0010033 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0030054 GO:0032870 GO:0033993 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043401 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048545 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060089 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

490

Amino Acids

54.62

Weight (kDa)

5.6

Isoelectric Point (pI)

33.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	71 - 309	5.6e-26	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	71 - 306	4.9e-22	Protein kinase domain
TPR_BSK1_C	PF25575	343 - 460	9.7e-62	Serine/threonine-protein kinase BSK1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007595)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G59010 AT5G59010
fragaria_vesca	FvH4_3g37400 FvH4_3g37400 FvH4_3g37400
malus_domestica	MD03G1081000.v1.1
prunus_persica	Prupe.6G065400_v2.0.a1 Prupe.6G065400_v2.0.a1 Prupe.6G065600_v2.0.a1 Prupe.6G065600_v2.0.a1 Prupe.6G065600_v2.0.a1 Prupe.6G065600_v2.0.a1
pyrus_communis	pycom11g07340
rosa_chinensis	RchiOBHm_Chr5g0067251
rosa_laevigata	RLG00000035919
rosa_multiflora	Rmu_sc0000015.1_g000015
rosa_roxburghii	Rroxscaffold_1G00013880
rosa_rugosa	Rorug05G0383200 Rorug05G0383300
rosa_samantha	Rh5AG442800 Rh5BG459900 Rh5CG482300 Rh5DG474800
rosa_wichuraiana	Rw5G041370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1012
Acc16I	TGCGCA	1 cut(s)	1416
AccB7I	CCANNNNNTGG	1 cut(s)	298
AccBSI	CCGCTC	1 cut(s)	337
AccI	GTMKAC	2 cut(s)	216, 1358
AccII	CGCG	1 cut(s)	289
AciI	CCGC	2 cut(s)	337, 1379
AclWI	GGATC	3 cut(s)	254, 1053, 1444
AcoI	YGGCCR	3 cut(s)	38, 238, 390
AcsI	RAATTY	2 cut(s)	346, 1226
AcuI	CTGAAG	1 cut(s)	57
AfaI	GTAC	5 cut(s)	533, 637, 665, 881, 962
AfeI	AGCGCT	1 cut(s)	260
AfiI	CCNNNNNNNGG	2 cut(s)	298, 1402
AgsI	TTSAA	5 cut(s)	121, 265, 938, 1156, 1376
AhlI	ACTAGT	1 cut(s)	1336
AjiI	CACGTC	1 cut(s)	156
AjnI	CCWGG	4 cut(s)	112, 132, 642, 1395
AjuI	GAANNNNNNNTTGG	4 cut(s)	323, 355, 632, 664
AloI	GAACNNNNNNTCC	2 cut(s)	167, 199
Alw21I	GWGCWC	2 cut(s)	10, 192
Alw26I	GTCTC	3 cut(s)	187, 915, 1142
AlwI	GGATC	3 cut(s)	254, 1053, 1444
AlwNI	CAGNNNCTG	1 cut(s)	1178
Ama87I	CYCGRG	1 cut(s)	1036
Aor51HI	AGCGCT	1 cut(s)	260
AoxI	GGCC	5 cut(s)	38, 238, 278, 390, 896
ApeKI	GCWGC	7 cut(s)	149, 322, 510, 1178, 1202, 1205, 1382
ApoI	RAATTY	2 cut(s)	346, 1226
ArsI	GACNNNNNNTTYG	2 cut(s)	893, 925
AspLEI	GCGC	4 cut(s)	261, 291, 1306, 1417
AspS9I	GGNCC	2 cut(s)	136, 714
AsuHPI	GGTGA	5 cut(s)	383, 643, 865, 1008, 1037
AvaI	CYCGRG	1 cut(s)	1036
AvaII	GGWCC	2 cut(s)	136, 714
BanII	GRGCYC	1 cut(s)	10
BarI	GAAGNNNNNNTAC	2 cut(s)	1067, 1099
BauI	CACGAG	1 cut(s)	888
BbsI	GAAGAC	1 cut(s)	240
Bbv12I	GWGCWC	2 cut(s)	10, 192
BbvCI	CCTCAGC	1 cut(s)	326
BbvI	GCAGC	7 cut(s)	136, 334, 522, 1165, 1189, 1192, 1369
BccI	CCATC	5 cut(s)	566, 796, 833, 964, 1396
BceAI	ACGGC	4 cut(s)	25, 225, 253, 637
BcgI	CGANNNNNNTGC	2 cut(s)	451, 485
BciT130I	CCWGG	4 cut(s)	114, 134, 644, 1397
BciVI	GTATCC	1 cut(s)	1076
BclI	TGATCA	1 cut(s)	565
BcoDI	GTCTC	3 cut(s)	187, 915, 1142
BcuI	ACTAGT	1 cut(s)	1336
BfaI	CTAG	3 cut(s)	309, 1022, 1337
BfmI	CTRYAG	1 cut(s)	631
BfoI	RGCGCY	1 cut(s)	262
BfuI	GTATCC	1 cut(s)	1076
BisI	GCNGC	8 cut(s)	150, 323, 511, 1179, 1203, 1206, 1380, 1383
BlpI	GCTNAGC	1 cut(s)	444
BlsI	GCNGC	8 cut(s)	151, 324, 512, 1180, 1204, 1207, 1381, 1384
BmcAI	AGTACT	1 cut(s)	665
Bme1390I	CCNGG	4 cut(s)	114, 134, 644, 1397
Bme18I	GGWCC	2 cut(s)	136, 714
BmeT110I	CYCGRG	1 cut(s)	1036
BmgBI	CACGTC	1 cut(s)	156
BmgT120I	GGNCC	2 cut(s)	136, 714
BmiI	GGNNCC	2 cut(s)	715, 954
BmrFI	CCNGG	4 cut(s)	114, 134, 644, 1397
BmsI	GCATC	6 cut(s)	433, 786, 860, 1165, 1312, 1402
BpiI	GAAGAC	1 cut(s)	240
BpmI	CTGGAG	2 cut(s)	320, 642
Bpu10I	CCTNAGC	1 cut(s)	326
Bpu1102I	GCTNAGC	1 cut(s)	444
BpuEI	CTTGAG	2 cut(s)	688, 1373
BsaAI	YACGTR	1 cut(s)	779
BsaBI	GATNNNNATC	1 cut(s)	576
BsaI	GGTCTC	1 cut(s)	187
BsaJI	CCNNGG	3 cut(s)	113, 1396, 1443
BsaXI	ACNNNNNCTCC	2 cut(s)	167, 197
Bsc4I	CCNNNNNNNGG	2 cut(s)	298, 1402
Bse1I	ACTGG	2 cut(s)	679, 1366
Bse3DI	GCAATG	1 cut(s)	1332
Bse8I	GATNNNNATC	1 cut(s)	576
BseBI	CCWGG	4 cut(s)	114, 134, 644, 1397
BseDI	CCNNGG	3 cut(s)	113, 1396, 1443
BseGI	GGATG	4 cut(s)	577, 1096, 1407, 1439
BseJI	GATNNNNATC	1 cut(s)	576
BseLI	CCNNNNNNNGG	2 cut(s)	298, 1402
BseMI	GCAATG	1 cut(s)	1332
BseMII	CTCAG	5 cut(s)	317, 458, 748, 837, 939
BseNI	ACTGG	2 cut(s)	679, 1366
BseX3I	CGGCCG	1 cut(s)	238
BseXI	GCAGC	7 cut(s)	136, 334, 522, 1165, 1189, 1192, 1369
Bsh1236I	CGCG	1 cut(s)	289
Bsh1285I	CGRYCG	1 cut(s)	241
BshFI	GGCC	5 cut(s)	40, 240, 280, 392, 898
BsiEI	CGRYCG	1 cut(s)	241
BsiHKAI	GWGCWC	2 cut(s)	10, 192
BsiHKCI	CYCGRG	1 cut(s)	1036
BsiWI	CGTACG	1 cut(s)	960
BslFI	GGGAC	1 cut(s)	727
BslI	CCNNNNNNNGG	2 cut(s)	298, 1402
BsmAI	GTCTC	3 cut(s)	187, 915, 1142
BsmFI	GGGAC	1 cut(s)	727
BsnI	GGCC	5 cut(s)	40, 240, 280, 392, 898
Bso31I	GGTCTC	1 cut(s)	187
BsoBI	CYCGRG	1 cut(s)	1036
Bsp1286I	GDGCHC	2 cut(s)	10, 192
Bsp143I	GATC	8 cut(s)	246, 269, 538, 565, 727, 1045, 1111, 1436
Bsp1720I	GCTNAGC	1 cut(s)	444
BspACI	CCGC	2 cut(s)	337, 1379
BspANI	GGCC	5 cut(s)	40, 240, 280, 392, 898
BspCNI	CTCAG	5 cut(s)	318, 457, 747, 838, 940
BspFNI	CGCG	1 cut(s)	289
BspLI	GGNNCC	2 cut(s)	715, 954
BspPI	GGATC	3 cut(s)	254, 1053, 1444
BspTNI	GGTCTC	1 cut(s)	187
BsrBI	CCGCTC	1 cut(s)	337
BsrDI	GCAATG	1 cut(s)	1332
BsrI	ACTGG	2 cut(s)	679, 1366
BssECI	CCNNGG	3 cut(s)	113, 1396, 1443
BssMI	GATC	8 cut(s)	246, 269, 538, 565, 727, 1045, 1111, 1436
BssSI	CACGAG	1 cut(s)	888
BssT1I	CCWWGG	1 cut(s)	1443
Bst2BI	CACGAG	1 cut(s)	888
Bst2UI	CCWGG	4 cut(s)	114, 134, 644, 1397
Bst4CI	ACNGT	3 cut(s)	718, 1238, 1261
Bst6I	CTCTTC	3 cut(s)	76, 673, 939
BstAPI	GCANNNNNTGC	1 cut(s)	1178
BstBAI	YACGTR	1 cut(s)	779
BstC8I	GCNNGC	5 cut(s)	525, 875, 1033, 1170, 1332
BstDEI	CTNAG	6 cut(s)	326, 420, 444, 734, 846, 948
BstF5I	GGATG	4 cut(s)	577, 1096, 1407, 1439
BstFNI	CGCG	1 cut(s)	289
BstH2I	RGCGCY	1 cut(s)	262
BstHHI	GCGC	4 cut(s)	261, 291, 1306, 1417
BstKTI	GATC	8 cut(s)	249, 272, 541, 568, 730, 1048, 1114, 1439
BstMAI	GTCTC	3 cut(s)	187, 915, 1142
BstMBI	GATC	8 cut(s)	246, 269, 538, 565, 727, 1045, 1111, 1436
BstMCI	CGRYCG	1 cut(s)	241
BstMWI	GCNNNNNNNGC	8 cut(s)	286, 621, 730, 1178, 1187, 1310, 1331, 1423
BstNI	CCWGG	4 cut(s)	114, 134, 644, 1397
BstNSI	RCATGY	1 cut(s)	1172
BstSCI	CCNGG	4 cut(s)	112, 132, 642, 1395
BstSFI	CTRYAG	1 cut(s)	631
BstUI	CGCG	1 cut(s)	289
BstV1I	GCAGC	7 cut(s)	136, 334, 522, 1165, 1189, 1192, 1369
BstV2I	GAAGAC	1 cut(s)	240
BstX2I	RGATCY	1 cut(s)	1045
BstYI	RGATCY	1 cut(s)	1045
BstZI	CGGCCG	1 cut(s)	238
BsuI	GTATCC	1 cut(s)	1076
BsuRI	GGCC	5 cut(s)	40, 240, 280, 392, 898
BtgZI	GCGATG	1 cut(s)	476
BtrI	CACGTC	1 cut(s)	156
BtsCI	GGATG	4 cut(s)	577, 1096, 1407, 1439
BtsIMutI	CAGTG	1 cut(s)	742
Cac8I	GCNNGC	5 cut(s)	525, 875, 1033, 1170, 1332
CaiI	CAGNNNCTG	1 cut(s)	1178
CfoI	GCGC	4 cut(s)	261, 291, 1306, 1417
Cfr13I	GGNCC	2 cut(s)	136, 714
Csp6I	GTAC	5 cut(s)	532, 636, 664, 880, 961
CviAII	CATG	8 cut(s)	400, 536, 761, 810, 983, 1061, 1169, 1300
CviQI	GTAC	5 cut(s)	532, 636, 664, 880, 961
DdeI	CTNAG	6 cut(s)	326, 420, 444, 734, 846, 948
DpnI	GATC	8 cut(s)	248, 271, 540, 567, 729, 1047, 1113, 1438
DpnII	GATC	8 cut(s)	246, 269, 538, 565, 727, 1045, 1111, 1436
DrdI	GACNNNNNNGTC	1 cut(s)	1012
DseDI	GACNNNNNNGTC	1 cut(s)	1012
EaeI	YGGCCR	3 cut(s)	38, 238, 390
EagI	CGGCCG	1 cut(s)	238
Eam1104I	CTCTTC	3 cut(s)	76, 673, 939
EarI	CTCTTC	3 cut(s)	76, 673, 939
Ecl136II	GAGCTC	1 cut(s)	8
EclXI	CGGCCG	1 cut(s)	238
Eco130I	CCWWGG	1 cut(s)	1443
Eco24I	GRGCYC	1 cut(s)	10
Eco31I	GGTCTC	1 cut(s)	187
Eco32I	GATATC	1 cut(s)	1251
Eco47I	GGWCC	2 cut(s)	136, 714
Eco47III	AGCGCT	1 cut(s)	260
Eco52I	CGGCCG	1 cut(s)	238
Eco53kI	GAGCTC	1 cut(s)	8
Eco57I	CTGAAG	1 cut(s)	57
Eco88I	CYCGRG	1 cut(s)	1036
EcoICRI	GAGCTC	1 cut(s)	8
EcoRI	GAATTC	1 cut(s)	1226
EcoRII	CCWGG	4 cut(s)	112, 132, 642, 1395
EcoRV	GATATC	1 cut(s)	1251
EcoT14I	CCWWGG	1 cut(s)	1443
EcoT38I	GRGCYC	1 cut(s)	10
ErhI	CCWWGG	1 cut(s)	1443
FaeI	CATG	8 cut(s)	403, 539, 764, 813, 986, 1064, 1172, 1303
FalI	AAGNNNNNCTT	4 cut(s)	413, 445, 1299, 1331
FaqI	GGGAC	1 cut(s)	727
FatI	CATG	8 cut(s)	399, 535, 760, 809, 982, 1060, 1168, 1299
FbaI	TGATCA	1 cut(s)	565
FblI	GTMKAC	2 cut(s)	216, 1358
Fnu4HI	GCNGC	8 cut(s)	150, 323, 511, 1179, 1203, 1206, 1380, 1383
FokI	GGATG	4 cut(s)	584, 1103, 1414, 1426
FriOI	GRGCYC	1 cut(s)	10
Fsp4HI	GCNGC	8 cut(s)	150, 323, 511, 1179, 1203, 1206, 1380, 1383
FspBI	CTAG	3 cut(s)	309, 1022, 1337
FspI	TGCGCA	1 cut(s)	1416
GlaI	GCGC	4 cut(s)	260, 290, 1305, 1416
GluI	GCNGC	8 cut(s)	150, 323, 511, 1179, 1203, 1206, 1380, 1383
GsuI	CTGGAG	2 cut(s)	320, 642
HaeII	RGCGCY	1 cut(s)	262
HaeIII	GGCC	5 cut(s)	40, 240, 280, 392, 898
HhaI	GCGC	4 cut(s)	261, 291, 1306, 1417
Hin1II	CATG	8 cut(s)	403, 539, 764, 813, 986, 1064, 1172, 1303
Hin6I	GCGC	4 cut(s)	259, 289, 1304, 1415
HinP1I	GCGC	4 cut(s)	259, 289, 1304, 1415
HincII	GTYRAC	1 cut(s)	1359
HindII	GTYRAC	1 cut(s)	1359
HindIII	AAGCTT	3 cut(s)	495, 1029, 1076
HphI	GGTGA	5 cut(s)	383, 643, 865, 1008, 1037
Hpy166II	GTNNAC	4 cut(s)	217, 703, 1131, 1359
Hpy188I	TCNGA	5 cut(s)	76, 172, 187, 999, 1005
Hpy188III	TCNNGA	8 cut(s)	159, 299, 569, 659, 867, 890, 1038, 1390
Hpy8I	GTNNAC	4 cut(s)	217, 703, 1131, 1359
Hpy99I	CGWCG	2 cut(s)	172, 1275
HpyAV	CCTTC	1 cut(s)	812
HpyCH4III	ACNGT	3 cut(s)	718, 1238, 1261
HpyCH4IV	ACGT	5 cut(s)	155, 177, 778, 963, 1273
HpyCH4V	TGCA	9 cut(s)	510, 764, 877, 980, 1055, 1172, 1181, 1325, 1330
HpyF10VI	GCNNNNNNNGC	8 cut(s)	286, 621, 730, 1178, 1187, 1310, 1331, 1423
HpyF3I	CTNAG	6 cut(s)	326, 420, 444, 734, 846, 948
HpySE526I	ACGT	5 cut(s)	155, 177, 778, 963, 1273
Hsp92II	CATG	8 cut(s)	403, 539, 764, 813, 986, 1064, 1172, 1303
HspAI	GCGC	4 cut(s)	259, 289, 1304, 1415
Ksp22I	TGATCA	1 cut(s)	565
Kzo9I	GATC	8 cut(s)	246, 269, 538, 565, 727, 1045, 1111, 1436
LmnI	GCTCC	3 cut(s)	5, 20, 208
Lsp1109I	GCAGC	7 cut(s)	136, 334, 522, 1165, 1189, 1192, 1369
LweI	GCATC	6 cut(s)	433, 786, 860, 1165, 1312, 1402
MaeI	CTAG	3 cut(s)	309, 1022, 1337
MaeII	ACGT	5 cut(s)	155, 177, 778, 963, 1273
MaeIII	GTNAC	5 cut(s)	371, 916, 1014, 1102, 1283
MalI	GATC	8 cut(s)	248, 271, 540, 567, 729, 1047, 1113, 1438
MbiI	CCGCTC	1 cut(s)	337
MboI	GATC	8 cut(s)	246, 269, 538, 565, 727, 1045, 1111, 1436
MboII	GAAGA	6 cut(s)	63, 245, 619, 690, 926, 1364
MflI	RGATCY	1 cut(s)	1045
MhlI	GDGCHC	2 cut(s)	10, 192
MluCI	AATT	3 cut(s)	293, 346, 1226
MlyI	GAGTC	3 cut(s)	62, 579, 800
MmeI	TCCRAC	3 cut(s)	150, 224, 1280
MseI	TTAA	2 cut(s)	255, 543
MslI	CAYNNNNRTG	1 cut(s)	1173
MspA1I	CMGCKG	1 cut(s)	325
MspR9I	CCNGG	4 cut(s)	114, 134, 644, 1397
MvaI	CCWGG	4 cut(s)	114, 134, 644, 1397
MvnI	CGCG	1 cut(s)	289
MwoI	GCNNNNNNNGC	8 cut(s)	286, 621, 730, 1178, 1187, 1310, 1331, 1423
NdeII	GATC	8 cut(s)	246, 269, 538, 565, 727, 1045, 1111, 1436
NlaIII	CATG	8 cut(s)	403, 539, 764, 813, 986, 1064, 1172, 1303
NlaIV	GGNNCC	2 cut(s)	715, 954
NmeAIII	GCCGAG	1 cut(s)	418
NmuCI	GTSAC	4 cut(s)	371, 916, 1014, 1102
NsbI	TGCGCA	1 cut(s)	1416
NspI	RCATGY	1 cut(s)	1172
PaeI	GCATGC	1 cut(s)	1172
PaeR7I	CTCGAG	1 cut(s)	1036
PcsI	WCGNNNNNNNCGW	1 cut(s)	183
PfeI	GAWTC	7 cut(s)	104, 162, 284, 577, 1000, 1214, 1365
Pfl23II	CGTACG	1 cut(s)	960
PflMI	CCANNNNNTGG	1 cut(s)	298
PkrI	GCNGC	8 cut(s)	151, 324, 512, 1180, 1204, 1207, 1381, 1384
PleI	GAGTC	3 cut(s)	62, 579, 800
PpsI	GAGTC	3 cut(s)	62, 579, 800
Ppu21I	YACGTR	1 cut(s)	779
Psp124BI	GAGCTC	1 cut(s)	10
Psp6I	CCWGG	4 cut(s)	112, 132, 642, 1395
PspGI	CCWGG	4 cut(s)	112, 132, 642, 1395
PspLI	CGTACG	1 cut(s)	960
PspN4I	GGNNCC	2 cut(s)	715, 954
PspPI	GGNCC	2 cut(s)	136, 714
PstNI	CAGNNNCTG	1 cut(s)	1178
PsuI	RGATCY	1 cut(s)	1045
PvuII	CAGCTG	1 cut(s)	325
RsaI	GTAC	5 cut(s)	533, 637, 665, 881, 962
RsaNI	GTAC	5 cut(s)	532, 636, 664, 880, 961
RseI	CAYNNNNRTG	1 cut(s)	1173
SacI	GAGCTC	1 cut(s)	10
SalI	GTCGAC	1 cut(s)	1357
SaqAI	TTAA	2 cut(s)	255, 543
SatI	GCNGC	8 cut(s)	150, 323, 511, 1179, 1203, 1206, 1380, 1383
Sau3AI	GATC	8 cut(s)	246, 269, 538, 565, 727, 1045, 1111, 1436
Sau96I	GGNCC	2 cut(s)	136, 714
ScaI	AGTACT	1 cut(s)	665
SchI	GAGTC	3 cut(s)	62, 579, 800
ScrFI	CCNGG	4 cut(s)	114, 134, 644, 1397
SduI	GDGCHC	2 cut(s)	10, 192
SfaNI	GCATC	6 cut(s)	433, 786, 860, 1165, 1312, 1402
SfcI	CTRYAG	1 cut(s)	631
Sfr274I	CTCGAG	1 cut(s)	1036
SinI	GGWCC	2 cut(s)	136, 714
SlaI	CTCGAG	1 cut(s)	1036
SmiMI	CAYNNNNRTG	1 cut(s)	1173
SmlI	CTYRAG	3 cut(s)	667, 1036, 1388
SmoI	CTYRAG	3 cut(s)	667, 1036, 1388
SpeI	ACTAGT	1 cut(s)	1336
SphI	GCATGC	1 cut(s)	1172
Sse9I	AATT	3 cut(s)	293, 346, 1226
SsiI	CCGC	2 cut(s)	337, 1379
SspI	AATATT	1 cut(s)	1068
SspMI	CTAG	3 cut(s)	309, 1022, 1337
SstI	GAGCTC	1 cut(s)	10
StyD4I	CCNGG	4 cut(s)	112, 132, 642, 1395
StyI	CCWWGG	1 cut(s)	1443
TaaI	ACNGT	3 cut(s)	718, 1238, 1261
TaiI	ACGT	5 cut(s)	158, 180, 781, 966, 1276
TaqI	TCGA	5 cut(s)	231, 1037, 1186, 1270, 1358
TasI	AATT	3 cut(s)	293, 346, 1226
TatI	WGTACW	1 cut(s)	663
TauI	GCSGC	1 cut(s)	1382
TfiI	GAWTC	7 cut(s)	104, 162, 284, 577, 1000, 1214, 1365
Tru1I	TTAA	2 cut(s)	255, 543
Tru9I	TTAA	2 cut(s)	255, 543
TscAI	CASTG	1 cut(s)	742
TseFI	GTSAC	4 cut(s)	371, 916, 1014, 1102
TseI	GCWGC	7 cut(s)	149, 322, 510, 1178, 1202, 1205, 1382
Tsp45I	GTSAC	4 cut(s)	371, 916, 1014, 1102
TspDTI	ATGAA	9 cut(s)	388, 422, 423, 467, 620, 1077, 1206, 1219, 1307
TspGWI	ACGGA	2 cut(s)	207, 1449
TspRI	CASTG	1 cut(s)	742
Van91I	CCANNNNNTGG	1 cut(s)	298
VpaK11BI	GGWCC	2 cut(s)	136, 714
XapI	RAATTY	2 cut(s)	346, 1226
XceI	RCATGY	1 cut(s)	1172
XhoI	CTCGAG	1 cut(s)	1036
XmiI	GTMKAC	2 cut(s)	216, 1358
XspI	CTAG	3 cut(s)	309, 1022, 1337
ZrmI	AGTACT	1 cut(s)	665

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.