Rorug05G0383200

phosphatidylinositol ceramide inositolphosphotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006031
Physical Location & Seq
Forward (+)
52013846 .. 52019327
5482 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0383200.1

Sequence Viewer

Length: 1086 bp
ATGTCTTCAACACTACTTCGCTCCCGTTTAGCCTCTCTCGCATCGAAGCGCGGTCGCCGTCTGTTGAGCTCCGACGTCGCTTCAAACCCTCCTAAGGACCCATTCGTTGCCGCCGAAACCCTCGCTTCCAATCGCACACCTCCTCCACCGCCACCGCCAAGCGCCGCCGCTGCTAGCGCGTCAACTAGAAAACCCTGGAGCTTTCTCAAGTACTCCCTCATTGGCGCTCTCACCGGAGCCACCGCTTCTGTTGCTTATGTCTCCTATGCTTACTCGATTGATGAAATAGCTGAAAAGACAAAGGCTCTGCGAGAAGCGCGTGCAAGTATCGAAATTCCAGATGATGCGTCTACTCTTGATAAATTCAAAACTATGCTCTACTCTTCTGCAATTATAGTGCCTGCTAAAGCAGCTGAAGCTTACCTTGATGCTAGGAGGGCAATTGAAGAACAAGTTCGAGGTTATACTGAACCATATGCGGAAAAGCTTCTTCCAGATTTGCTTCCCATGGAGAGAAATGTTTATACACTTGTTCTGGATCTACAGGAGACATTACTTTACTCTTATTGGACGAGGGAAAAGGGCTGGCAGACGATCAAAAGACCTGGAGTTGATGCTTTCTTAGAGCATCTTGCTCAGTATTATGAAATCATCGTATTTTCGGACTATTCAAATATGTATGTAGATCCTGTAATGGAGAGATTGGACCCAAAACATTGTGTAAGATTCAGGCTAGGAAAGGCTGCCACCAAGTATCAAGATGGAGTGCATTATAGGGACCTTTCAAAGCTAAATAGAGATCCAAGAAAGATCATATATCTGAGTGCCCATGCACGAGAAAATAGCCTACAGCCTGAAAATGGTGCCGTAATAAAGCCATATAAGTCTGAGCTGGATGATACAGCGCTTGTAGATTTCATCCCATTTCTTGAATTTGTCGCCCGTAACCCTCCAGCAGATATCAGACAAGTATTAGCATCGTATGAAGGACATGAAATTCCAGCTGAGTTCATTAGGCGTACTAAGGAGCATCAGAGGAAAATGCAAGAACAGAAGCAGCATGGTCCTCGACTCTGGAGGCGTTGA

Protein Analysis

361

Amino Acids

41.01

Weight (kDa)

9.17

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 175 - 315 1.9e-35 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 78
AccB1I GGYRCC 1 cut(s) 863
AccI GTMKAC 1 cut(s) 350
AccII CGCG 3 cut(s) 51, 179, 319
AciI CCGC 8 cut(s) 51, 111, 149, 155, 165, 168, 243, 479
AclWI GGATC 3 cut(s) 546, 680, 794
AcsI RAATTY 4 cut(s) 333, 362, 932, 996
AcuI CTGAAG 1 cut(s) 435
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 2 cut(s) 212, 1021
AfeI AGCGCT 1 cut(s) 906
AfiI CCNNNNNNNGG 2 cut(s) 94, 860
AgsI TTSAA 7 cut(s) 9, 84, 367, 446, 672, 786, 932
AjnI CCWGG 2 cut(s) 194, 604
AluBI AGCT 9 cut(s) 69, 201, 290, 413, 419, 487, 790, 892, 1004
AluI AGCT 9 cut(s) 69, 201, 290, 413, 419, 487, 790, 892, 1004
Alw21I GWGCWC 1 cut(s) 71
Alw26I GTCTC 2 cut(s) 265, 542
AlwI GGATC 3 cut(s) 546, 680, 794
Aor51HI AGCGCT 1 cut(s) 906
ApeKI GCWGC 4 cut(s) 170, 410, 743, 1057
ApoI RAATTY 4 cut(s) 333, 362, 932, 996
Asp700I GAANNNNTTC 2 cut(s) 453, 486
AspLEI GCGC 6 cut(s) 51, 164, 179, 227, 319, 907
AspS9I GGNCC 4 cut(s) 97, 706, 778, 1064
AsuHPI GGTGA 1 cut(s) 223
AsuNHI GCTAGC 1 cut(s) 173
AvaII GGWCC 4 cut(s) 97, 706, 778, 1064
AxyI CCTNAGG 1 cut(s) 93
BaeGI GKGCMC 1 cut(s) 829
BanI GGYRCC 1 cut(s) 863
BanII GRGCYC 1 cut(s) 71
BauI CACGAG 1 cut(s) 834
Bbv12I GWGCWC 1 cut(s) 71
BbvI GCAGC 4 cut(s) 157, 422, 730, 1069
BccI CCATC 1 cut(s) 755
BceAI ACGGC 2 cut(s) 42, 851
BcgI CGANNNNNNTGC 2 cut(s) 1049, 1083
BciT130I CCWGG 2 cut(s) 196, 606
BcoDI GTCTC 2 cut(s) 265, 542
BfaI CTAG 4 cut(s) 174, 186, 432, 734
BfmI CTRYAG 2 cut(s) 542, 848
BfoI RGCGCY 3 cut(s) 165, 228, 908
BisI GCNGC 7 cut(s) 111, 165, 168, 171, 411, 744, 1058
BlsI GCNGC 7 cut(s) 112, 166, 169, 172, 412, 745, 1059
BmcAI AGTACT 1 cut(s) 212
Bme1390I CCNGG 2 cut(s) 196, 606
Bme18I GGWCC 4 cut(s) 97, 706, 778, 1064
BmgT120I GGNCC 4 cut(s) 97, 706, 778, 1064
BmiI GGNNCC 5 cut(s) 99, 238, 708, 779, 865
BmrFI CCNGG 2 cut(s) 196, 606
BmsI GCATC 7 cut(s) 50, 334, 418, 604, 637, 986, 1039
BmtI GCTAGC 1 cut(s) 177
BpmI CTGGAG 3 cut(s) 217, 627, 936
BpuEI CTTGAG 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 2 cut(s) 194, 507
BsaWI WCCGGW 1 cut(s) 233
BsaXI ACNNNNNCTCC 2 cut(s) 127, 157
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 860
Bse21I CCTNAGG 1 cut(s) 93
BseBI CCWGG 2 cut(s) 196, 606
BseDI CCNNGG 2 cut(s) 194, 507
BseGI GGATG 2 cut(s) 901, 918
BseLI CCNNNNNNNGG 2 cut(s) 94, 860
BseMII CTCAG 4 cut(s) 650, 812, 879, 996
BseRI GAGGAG 1 cut(s) 132
BseSI GKGCMC 1 cut(s) 829
BseXI GCAGC 4 cut(s) 157, 422, 730, 1069
Bsh1236I CGCG 3 cut(s) 51, 179, 319
Bsh1285I CGRYCG 1 cut(s) 55
BshNI GGYRCC 1 cut(s) 863
BsiEI CGRYCG 1 cut(s) 55
BsiHKAI GWGCWC 1 cut(s) 71
BsiSI CCGG 1 cut(s) 234
BslFI GGGAC 1 cut(s) 791
BslI CCNNNNNNNGG 2 cut(s) 94, 860
BsmAI GTCTC 2 cut(s) 265, 542
BsmFI GGGAC 1 cut(s) 791
Bsp1286I GDGCHC 2 cut(s) 71, 829
Bsp143I GATC 5 cut(s) 538, 594, 685, 799, 810
Bsp19I CCATGG 1 cut(s) 507
BspACI CCGC 8 cut(s) 51, 111, 149, 155, 165, 168, 243, 479
BspCNI CTCAG 4 cut(s) 649, 813, 880, 997
BspFNI CGCG 3 cut(s) 51, 179, 319
BspLI GGNNCC 5 cut(s) 99, 238, 708, 779, 865
BspOI GCTAGC 1 cut(s) 177
BspPI GGATC 3 cut(s) 546, 680, 794
BspT107I GGYRCC 1 cut(s) 863
BssECI CCNNGG 2 cut(s) 194, 507
BssMI GATC 5 cut(s) 538, 594, 685, 799, 810
BssNI GRCGYC 1 cut(s) 75
BssSI CACGAG 1 cut(s) 834
BssT1I CCWWGG 1 cut(s) 507
Bst2BI CACGAG 1 cut(s) 834
Bst2UI CCWGG 2 cut(s) 196, 606
Bst6I CTCTTC 1 cut(s) 388
BstACI GRCGYC 1 cut(s) 75
BstC8I GCNNGC 4 cut(s) 175, 321, 402, 587
BstDEI CTNAG 7 cut(s) 93, 622, 636, 821, 888, 1005, 1023
BstDSI CCRYGG 1 cut(s) 507
BstF5I GGATG 2 cut(s) 901, 918
BstFNI CGCG 3 cut(s) 51, 179, 319
BstH2I RGCGCY 3 cut(s) 165, 228, 908
BstHHI GCGC 6 cut(s) 51, 164, 179, 227, 319, 907
BstKTI GATC 5 cut(s) 541, 597, 688, 802, 813
BstMAI GTCTC 2 cut(s) 265, 542
BstMBI GATC 5 cut(s) 538, 594, 685, 799, 810
BstMCI CGRYCG 1 cut(s) 55
BstMWI GCNNNNNNNGC 8 cut(s) 38, 170, 176, 251, 316, 410, 416, 437
BstNI CCWGG 2 cut(s) 196, 606
BstSCI CCNGG 2 cut(s) 194, 604
BstSFI CTRYAG 2 cut(s) 542, 848
BstSLI GKGCMC 1 cut(s) 829
BstUI CGCG 3 cut(s) 51, 179, 319
BstV1I GCAGC 4 cut(s) 157, 422, 730, 1069
BstX2I RGATCY 3 cut(s) 538, 685, 799
BstYI RGATCY 3 cut(s) 538, 685, 799
Bsu36I CCTNAGG 1 cut(s) 93
BtgI CCRYGG 1 cut(s) 507
BtsCI GGATG 2 cut(s) 901, 918
Cac8I GCNNGC 4 cut(s) 175, 321, 402, 587
CfoI GCGC 6 cut(s) 51, 164, 179, 227, 319, 907
Cfr13I GGNCC 4 cut(s) 97, 706, 778, 1064
CseI GACGC 2 cut(s) 168, 336
Csp6I GTAC 2 cut(s) 211, 1020
CviAII CATG 4 cut(s) 508, 830, 992, 1061
CviQI GTAC 2 cut(s) 211, 1020
DdeI CTNAG 7 cut(s) 93, 622, 636, 821, 888, 1005, 1023
DpnI GATC 5 cut(s) 540, 596, 687, 801, 812
DpnII GATC 5 cut(s) 538, 594, 685, 799, 810
Eam1104I CTCTTC 1 cut(s) 388
EarI CTCTTC 1 cut(s) 388
Ecl136II GAGCTC 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 507
Eco24I GRGCYC 1 cut(s) 71
Eco32I GATATC 1 cut(s) 961
Eco47I GGWCC 4 cut(s) 97, 706, 778, 1064
Eco47III AGCGCT 1 cut(s) 906
Eco53kI GAGCTC 1 cut(s) 69
Eco57I CTGAAG 1 cut(s) 435
Eco81I CCTNAGG 1 cut(s) 93
EcoICRI GAGCTC 1 cut(s) 69
EcoO109I RGGNCCY 2 cut(s) 97, 778
EcoRII CCWGG 2 cut(s) 194, 604
EcoRV GATATC 1 cut(s) 961
EcoT14I CCWWGG 1 cut(s) 507
EcoT38I GRGCYC 1 cut(s) 71
ErhI CCWWGG 1 cut(s) 507
FaeI CATG 4 cut(s) 511, 833, 995, 1064
FalI AAGNNNNNCTT 2 cut(s) 408, 440
FaqI GGGAC 1 cut(s) 791
FatI CATG 4 cut(s) 507, 829, 991, 1060
FauNDI CATATG 1 cut(s) 475
FblI GTMKAC 1 cut(s) 350
Fnu4HI GCNGC 7 cut(s) 111, 165, 168, 171, 411, 744, 1058
FokI GGATG 2 cut(s) 905, 908
FriOI GRGCYC 1 cut(s) 71
Fsp4HI GCNGC 7 cut(s) 111, 165, 168, 171, 411, 744, 1058
FspBI CTAG 4 cut(s) 174, 186, 432, 734
GlaI GCGC 6 cut(s) 50, 163, 178, 226, 318, 906
GluI GCNGC 7 cut(s) 111, 165, 168, 171, 411, 744, 1058
GsuI CTGGAG 3 cut(s) 217, 627, 936
HaeII RGCGCY 3 cut(s) 165, 228, 908
HapII CCGG 1 cut(s) 234
HgaI GACGC 2 cut(s) 168, 336
HhaI GCGC 6 cut(s) 51, 164, 179, 227, 319, 907
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 4 cut(s) 511, 833, 995, 1064
Hin6I GCGC 6 cut(s) 49, 162, 177, 225, 317, 905
HinP1I GCGC 6 cut(s) 49, 162, 177, 225, 317, 905
HincII GTYRAC 1 cut(s) 183
HindII GTYRAC 1 cut(s) 183
HindIII AAGCTT 2 cut(s) 417, 485
HinfI GANTC 2 cut(s) 726, 1071
HpaII CCGG 1 cut(s) 234
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 2 cut(s) 183, 351
Hpy188I TCNGA 6 cut(s) 73, 664, 822, 889, 965, 1035
Hpy188III TCNNGA 7 cut(s) 338, 356, 494, 536, 758, 929, 1075
Hpy8I GTNNAC 2 cut(s) 183, 351
Hpy99I CGWCG 2 cut(s) 77, 80
HpyAV CCTTC 1 cut(s) 980
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 5 cut(s) 323, 389, 769, 833, 1045
HpyF10VI GCNNNNNNNGC 8 cut(s) 38, 170, 176, 251, 316, 410, 416, 437
HpyF3I CTNAG 7 cut(s) 93, 622, 636, 821, 888, 1005, 1023
HpySE526I ACGT 1 cut(s) 75
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 4 cut(s) 511, 833, 995, 1064
HspAI GCGC 6 cut(s) 49, 162, 177, 225, 317, 905
Kzo9I GATC 5 cut(s) 538, 594, 685, 799, 810
LmnI GCTCC 5 cut(s) 26, 74, 198, 236, 1027
Lsp1109I GCAGC 4 cut(s) 157, 422, 730, 1069
LweI GCATC 7 cut(s) 50, 334, 418, 604, 637, 986, 1039
MaeI CTAG 4 cut(s) 174, 186, 432, 734
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 1 cut(s) 944
MalI GATC 5 cut(s) 540, 596, 687, 801, 812
MboI GATC 5 cut(s) 538, 594, 685, 799, 810
MboII GAAGA 3 cut(s) 375, 458, 482
MfeI CAATTG 1 cut(s) 441
MflI RGATCY 3 cut(s) 538, 685, 799
MhlI GDGCHC 2 cut(s) 71, 829
MluCI AATT 6 cut(s) 333, 362, 390, 441, 932, 996
MlyI GAGTC 1 cut(s) 1065
MmeI TCCRAC 1 cut(s) 96
MroXI GAANNNNTTC 2 cut(s) 453, 486
MspA1I CMGCKG 3 cut(s) 170, 413, 1004
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 2 cut(s) 196, 606
MunI CAATTG 1 cut(s) 441
MvaI CCWGG 2 cut(s) 196, 606
MvnI CGCG 3 cut(s) 51, 179, 319
MwoI GCNNNNNNNGC 8 cut(s) 38, 170, 176, 251, 316, 410, 416, 437
NcoI CCATGG 1 cut(s) 507
NdeI CATATG 1 cut(s) 475
NdeII GATC 5 cut(s) 538, 594, 685, 799, 810
NheI GCTAGC 1 cut(s) 173
NlaIII CATG 4 cut(s) 511, 833, 995, 1064
NlaIV GGNNCC 5 cut(s) 99, 238, 708, 779, 865
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PdmI GAANNNNTTC 2 cut(s) 453, 486
PfeI GAWTC 1 cut(s) 726
PkrI GCNGC 7 cut(s) 112, 166, 169, 172, 412, 745, 1059
PleI GAGTC 1 cut(s) 1065
PpsI GAGTC 1 cut(s) 1065
PpuMI RGGWCCY 2 cut(s) 97, 778
Psp124BI GAGCTC 1 cut(s) 71
Psp5II RGGWCCY 2 cut(s) 97, 778
Psp6I CCWGG 2 cut(s) 194, 604
PspGI CCWGG 2 cut(s) 194, 604
PspN4I GGNNCC 5 cut(s) 99, 238, 708, 779, 865
PspPI GGNCC 4 cut(s) 97, 706, 778, 1064
PspPPI RGGWCCY 2 cut(s) 97, 778
PsuI RGATCY 3 cut(s) 538, 685, 799
PvuII CAGCTG 2 cut(s) 413, 1004
RsaI GTAC 2 cut(s) 212, 1021
RsaNI GTAC 2 cut(s) 211, 1020
SacI GAGCTC 1 cut(s) 71
SatI GCNGC 7 cut(s) 111, 165, 168, 171, 411, 744, 1058
Sau3AI GATC 5 cut(s) 538, 594, 685, 799, 810
Sau96I GGNCC 4 cut(s) 97, 706, 778, 1064
ScaI AGTACT 1 cut(s) 212
SchI GAGTC 1 cut(s) 1065
ScrFI CCNGG 2 cut(s) 196, 606
SduI GDGCHC 2 cut(s) 71, 829
SfaNI GCATC 7 cut(s) 50, 334, 418, 604, 637, 986, 1039
SfcI CTRYAG 2 cut(s) 542, 848
SinI GGWCC 4 cut(s) 97, 706, 778, 1064
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 6 cut(s) 333, 362, 390, 441, 932, 996
SsiI CCGC 8 cut(s) 51, 111, 149, 155, 165, 168, 243, 479
SspMI CTAG 4 cut(s) 174, 186, 432, 734
SstI GAGCTC 1 cut(s) 71
StyD4I CCNGG 2 cut(s) 194, 604
StyI CCWWGG 1 cut(s) 507
TaiI ACGT 1 cut(s) 78
TaqI TCGA 5 cut(s) 44, 275, 330, 457, 1069
TasI AATT 6 cut(s) 333, 362, 390, 441, 932, 996
TatI WGTACW 1 cut(s) 210
TauI GCSGC 3 cut(s) 113, 167, 170
TfiI GAWTC 1 cut(s) 726
TseI GCWGC 4 cut(s) 170, 410, 743, 1057
TspDTI ATGAA 6 cut(s) 297, 660, 907, 999, 1000, 1008
VpaK11BI GGWCC 4 cut(s) 97, 706, 778, 1064
XapI RAATTY 4 cut(s) 333, 362, 932, 996
XmiI GTMKAC 1 cut(s) 350
XmnI GAANNNNTTC 2 cut(s) 453, 486
XspI CTAG 4 cut(s) 174, 186, 432, 734
ZraI GACGTC 1 cut(s) 76
ZrmI AGTACT 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.