MD03G1081100.v1.1

phosphatidylinositol ceramide inositolphosphotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006031
Physical Location & Seq
Reverse (-)
6539109 .. 6542565
3457 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1081100.v1.1.491

Sequence Viewer

Length: 861 bp
ATGCCGTTTATCTTTGCTCGCGAGGCTCCTAAGCTATGGAGGAAAGTATGCGCAGAGACGTCAACTGAGCTTTCGCTTCTCGCCGGAAATTGGAAGTTTGTTCTTGCTGGTCTCATTTTTCAGTATCTCCATGGGGTAGCCGCTCATGGAGTCCATTATTTACATCAGCCAGGACCAACGCTTCAGGATGCTGGGTTCTTTCTGCTCCCGGAACTTGGTCAAGCAAAAAATTATCTGAGTGAGACTTTCTTCGCTGTCATCTTCTTTTCCTTTATCTTGTGGACATTTCACCCTTTTGTTTTCCAGTTGAAAAAGATATATACAGTTCTGATATGGTGCAGAGTATTTGTGTATTTAGCCGTGTGTCAAATGCTCCGGATCATCACCTTCTACTCTACTCTACTTCCTGGTCCAAGCTACCATTGTCGTGAGGGCTCTAATCTTGCCAGGCTCCCCCCTCCAGAGAGTGCACTTGAAGTGCTCTTAATCAACTTTCCCCAAGGCATTGTGTATGGTTGTGGTGATTTGATTTTTTCATCGCACATGATTTTTACCTTAGTATTTGTGCGCACATATCAGAAATATGGAACAAGGAGGTGCTTTAAGCAGCTTGCTTGGTCAGTTGCTGTGGTTCAGAGCCTCCTGATTCTAACTTCTCGCAAACATTACACAGTGGATGTTGTTGTTGCCTGGTATACCGTGAATTTGGTAGTGTTCTTTATAGACCAAAAATTGCCAGAACTGCCTGATCGATCTACATCTCAACCACTGCTACCAATGAGCACCAGAGAGAAGGATAGCAAGAACAGAGAAGAGCTTGACAAATTCCTTAACGGAAATTCTGCCAACATTACCACTTGA

Protein Analysis

287

Amino Acids

32.81

Weight (kDa)

9.08

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP2_C PF14360 172 - 240 1.2e-22 PAP2 superfamily C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 62
Acc16I TGCGCA 2 cut(s) 52, 569
AccBSI CCGCTC 1 cut(s) 143
AccI GTMKAC 1 cut(s) 695
AccII CGCG 1 cut(s) 21
AccIII TCCGGA 1 cut(s) 375
AciI CCGC 1 cut(s) 141
AclWI GGATC 1 cut(s) 386
AcsI RAATTY 3 cut(s) 703, 824, 838
AcuI CTGAAG 1 cut(s) 167
AcyI GRCGYC 1 cut(s) 59
AfiI CCNNNNNNNGG 2 cut(s) 90, 215
AgsI TTSAA 2 cut(s) 310, 476
AjnI CCWGG 4 cut(s) 169, 406, 446, 689
AloI GAACNNNNNNTCC 2 cut(s) 179, 211
AluBI AGCT 5 cut(s) 34, 70, 417, 610, 817
AluI AGCT 5 cut(s) 34, 70, 417, 610, 817
Alw21I GWGCWC 3 cut(s) 472, 483, 785
Alw26I GTCTC 3 cut(s) 50, 116, 236
Alw44I GTGCAC 1 cut(s) 468
AlwI GGATC 1 cut(s) 386
AlwNI CAGNNNCTG 1 cut(s) 626
Aor13HI TCCGGA 1 cut(s) 375
ApaLI GTGCAC 1 cut(s) 468
ApeKI GCWGC 1 cut(s) 607
ApoI RAATTY 3 cut(s) 703, 824, 838
AspLEI GCGC 2 cut(s) 53, 570
AspS9I GGNCC 2 cut(s) 173, 410
AsuC2I CCSGG 1 cut(s) 209
AsuHPI GGTGA 3 cut(s) 281, 376, 533
AvaII GGWCC 2 cut(s) 173, 410
BaeGI GKGCMC 1 cut(s) 472
BanII GRGCYC 1 cut(s) 437
Bbv12I GWGCWC 3 cut(s) 472, 483, 785
BbvI GCAGC 1 cut(s) 619
BceAI ACGGC 1 cut(s) 344
BciT130I CCWGG 4 cut(s) 171, 408, 448, 691
BcnI CCSGG 1 cut(s) 209
BcoDI GTCTC 3 cut(s) 50, 116, 236
BisI GCNGC 2 cut(s) 141, 608
BlsI GCNGC 2 cut(s) 142, 609
Bme1390I CCNGG 5 cut(s) 171, 209, 408, 448, 691
Bme18I GGWCC 2 cut(s) 173, 410
BmgT120I GGNCC 2 cut(s) 173, 410
BmiI GGNNCC 2 cut(s) 27, 452
BmrFI CCNGG 5 cut(s) 171, 209, 408, 448, 691
BmsI GCATC 1 cut(s) 178
BpmI CTGGAG 1 cut(s) 444
Bpu10I CCTNAGC 1 cut(s) 30
BpuMI CCSGG 1 cut(s) 209
Bsa29I ATCGAT 1 cut(s) 751
BsaBI GATNNNNATC 1 cut(s) 757
BsaHI GRCGYC 1 cut(s) 59
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 2 cut(s) 130, 499
BsaWI WCCGGW 1 cut(s) 375
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 215
Bse1I ACTGG 1 cut(s) 304
Bse8I GATNNNNATC 1 cut(s) 757
BseAI TCCGGA 1 cut(s) 375
BseBI CCWGG 4 cut(s) 171, 408, 448, 691
BseCI ATCGAT 1 cut(s) 751
BseDI CCNNGG 2 cut(s) 130, 499
BseGI GGATG 2 cut(s) 193, 682
BseJI GATNNNNATC 1 cut(s) 757
BseLI CCNNNNNNNGG 2 cut(s) 90, 215
BseMII CTCAG 2 cut(s) 57, 227
BseNI ACTGG 1 cut(s) 304
BseSI GKGCMC 1 cut(s) 472
BseXI GCAGC 1 cut(s) 619
BseYI CCCAGC 1 cut(s) 191
BsgI GTGCAG 1 cut(s) 358
Bsh1236I CGCG 1 cut(s) 21
BshVI ATCGAT 1 cut(s) 751
BsiHKAI GWGCWC 3 cut(s) 472, 483, 785
BsiSI CCGG 3 cut(s) 84, 209, 376
BslI CCNNNNNNNGG 2 cut(s) 90, 215
BsmAI GTCTC 3 cut(s) 50, 116, 236
BsmBI CGTCTC 1 cut(s) 50
Bso31I GGTCTC 1 cut(s) 116
Bsp1286I GDGCHC 4 cut(s) 437, 472, 483, 785
Bsp13I TCCGGA 1 cut(s) 375
Bsp143I GATC 3 cut(s) 378, 748, 752
Bsp19I CCATGG 1 cut(s) 130
Bsp68I TCGCGA 1 cut(s) 21
BspACI CCGC 1 cut(s) 141
BspCNI CTCAG 2 cut(s) 58, 228
BspDI ATCGAT 1 cut(s) 751
BspEI TCCGGA 1 cut(s) 375
BspFNI CGCG 1 cut(s) 21
BspLI GGNNCC 2 cut(s) 27, 452
BspPI GGATC 1 cut(s) 386
BspQI GCTCTTC 1 cut(s) 807
BspTNI GGTCTC 1 cut(s) 116
BsrBI CCGCTC 1 cut(s) 143
BsrI ACTGG 1 cut(s) 304
BssECI CCNNGG 2 cut(s) 130, 499
BssMI GATC 3 cut(s) 378, 748, 752
BssNAI GTATAC 1 cut(s) 696
BssNI GRCGYC 1 cut(s) 59
BssT1I CCWWGG 2 cut(s) 130, 499
Bst1107I GTATAC 1 cut(s) 696
Bst2UI CCWGG 4 cut(s) 171, 408, 448, 691
Bst4CI ACNGT 3 cut(s) 325, 673, 700
Bst6I CTCTTC 1 cut(s) 807
BstACI GRCGYC 1 cut(s) 59
BstC8I GCNNGC 2 cut(s) 19, 612
BstDEI CTNAG 4 cut(s) 30, 66, 236, 556
BstDSI CCRYGG 1 cut(s) 130
BstF5I GGATG 2 cut(s) 193, 682
BstFNI CGCG 1 cut(s) 21
BstHHI GCGC 2 cut(s) 53, 570
BstKTI GATC 3 cut(s) 381, 751, 755
BstMAI GTCTC 3 cut(s) 50, 116, 236
BstMBI GATC 3 cut(s) 378, 748, 752
BstMWI GCNNNNNNNGC 2 cut(s) 23, 742
BstNI CCWGG 4 cut(s) 171, 408, 448, 691
BstSCI CCNGG 5 cut(s) 169, 207, 406, 446, 689
BstSLI GKGCMC 1 cut(s) 472
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 619
BstZ17I GTATAC 1 cut(s) 696
Bsu15I ATCGAT 1 cut(s) 751
BsuTUI ATCGAT 1 cut(s) 751
BtgI CCRYGG 1 cut(s) 130
BtgZI GCGATG 1 cut(s) 522
BtsCI GGATG 2 cut(s) 193, 682
BtsI GCAGTG 1 cut(s) 767
BtsIMutI CAGTG 2 cut(s) 678, 767
BtuMI TCGCGA 1 cut(s) 21
Cac8I GCNNGC 2 cut(s) 19, 612
CaiI CAGNNNCTG 1 cut(s) 626
CfoI GCGC 2 cut(s) 53, 570
Cfr13I GGNCC 2 cut(s) 173, 410
ClaI ATCGAT 1 cut(s) 751
CviAII CATG 3 cut(s) 131, 146, 544
DdeI CTNAG 4 cut(s) 30, 66, 236, 556
DpnI GATC 3 cut(s) 380, 750, 754
DpnII GATC 3 cut(s) 378, 748, 752
Eam1104I CTCTTC 1 cut(s) 807
EarI CTCTTC 1 cut(s) 807
Eco130I CCWWGG 2 cut(s) 130, 499
Eco24I GRGCYC 1 cut(s) 437
Eco31I GGTCTC 1 cut(s) 116
Eco47I GGWCC 2 cut(s) 173, 410
Eco57I CTGAAG 1 cut(s) 167
EcoRII CCWGG 4 cut(s) 169, 406, 446, 689
EcoT14I CCWWGG 2 cut(s) 130, 499
EcoT38I GRGCYC 1 cut(s) 437
ErhI CCWWGG 2 cut(s) 130, 499
Esp3I CGTCTC 1 cut(s) 50
FaeI CATG 3 cut(s) 134, 149, 547
FatI CATG 3 cut(s) 130, 145, 543
FblI GTMKAC 1 cut(s) 695
Fnu4HI GCNGC 2 cut(s) 141, 608
FokI GGATG 2 cut(s) 200, 689
FriOI GRGCYC 1 cut(s) 437
Fsp4HI GCNGC 2 cut(s) 141, 608
FspAI RTGCGCAY 1 cut(s) 569
FspI TGCGCA 2 cut(s) 52, 569
GlaI GCGC 2 cut(s) 52, 569
GluI GCNGC 2 cut(s) 141, 608
GsaI CCCAGC 1 cut(s) 195
GsuI CTGGAG 1 cut(s) 444
HapII CCGG 3 cut(s) 84, 209, 376
HhaI GCGC 2 cut(s) 53, 570
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 3 cut(s) 134, 149, 547
Hin6I GCGC 2 cut(s) 51, 568
HinP1I GCGC 2 cut(s) 51, 568
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HinfI GANTC 2 cut(s) 150, 646
HpaII CCGG 3 cut(s) 84, 209, 376
HphI GGTGA 3 cut(s) 281, 376, 533
Hpy166II GTNNAC 4 cut(s) 63, 282, 470, 696
Hpy188I TCNGA 4 cut(s) 237, 330, 579, 636
Hpy188III TCNNGA 6 cut(s) 20, 185, 376, 428, 461, 643
Hpy8I GTNNAC 4 cut(s) 63, 282, 470, 696
HpyAV CCTTC 2 cut(s) 397, 787
HpyCH4III ACNGT 3 cut(s) 325, 673, 700
HpyCH4IV ACGT 1 cut(s) 59
HpyCH4V TGCA 2 cut(s) 339, 470
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 742
HpyF3I CTNAG 4 cut(s) 30, 66, 236, 556
HpySE526I ACGT 1 cut(s) 59
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 3 cut(s) 134, 149, 547
HspAI GCGC 2 cut(s) 51, 568
Kpn2I TCCGGA 1 cut(s) 375
Kzo9I GATC 3 cut(s) 378, 748, 752
LguI GCTCTTC 1 cut(s) 807
LmnI GCTCC 4 cut(s) 31, 210, 378, 456
Lsp1109I GCAGC 1 cut(s) 619
LweI GCATC 1 cut(s) 178
MaeII ACGT 1 cut(s) 59
MalI GATC 3 cut(s) 380, 750, 754
MbiI CCGCTC 1 cut(s) 143
MboI GATC 3 cut(s) 378, 748, 752
MboII GAAGA 3 cut(s) 241, 253, 824
MhlI GDGCHC 4 cut(s) 437, 472, 483, 785
MluCI AATT 6 cut(s) 88, 229, 703, 731, 824, 838
MlyI GAGTC 1 cut(s) 159
MnlI CCTC 6 cut(s) 16, 33, 424, 468, 588, 650
MroI TCCGGA 1 cut(s) 375
MseI TTAA 3 cut(s) 485, 603, 831
MslI CAYNNNNRTG 1 cut(s) 426
MspI CCGG 3 cut(s) 84, 209, 376
MspR9I CCNGG 5 cut(s) 171, 209, 408, 448, 691
MvaI CCWGG 4 cut(s) 171, 408, 448, 691
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 2 cut(s) 23, 742
NciI CCSGG 1 cut(s) 209
NcoI CCATGG 1 cut(s) 130
NdeII GATC 3 cut(s) 378, 748, 752
NlaIII CATG 3 cut(s) 134, 149, 547
NlaIV GGNNCC 2 cut(s) 27, 452
NruI TCGCGA 1 cut(s) 21
NsbI TGCGCA 2 cut(s) 52, 569
PciSI GCTCTTC 1 cut(s) 807
PfeI GAWTC 1 cut(s) 646
PfoI TCCNGGA 1 cut(s) 207
PkrI GCNGC 2 cut(s) 142, 609
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
Psp6I CCWGG 4 cut(s) 169, 406, 446, 689
PspFI CCCAGC 1 cut(s) 191
PspGI CCWGG 4 cut(s) 169, 406, 446, 689
PspN4I GGNNCC 2 cut(s) 27, 452
PspPI GGNCC 2 cut(s) 173, 410
PstNI CAGNNNCTG 1 cut(s) 626
RruI TCGCGA 1 cut(s) 21
RseI CAYNNNNRTG 1 cut(s) 426
SapI GCTCTTC 1 cut(s) 807
SaqAI TTAA 3 cut(s) 485, 603, 831
SatI GCNGC 2 cut(s) 141, 608
Sau3AI GATC 3 cut(s) 378, 748, 752
Sau96I GGNCC 2 cut(s) 173, 410
SchI GAGTC 1 cut(s) 159
ScrFI CCNGG 5 cut(s) 171, 209, 408, 448, 691
SduI GDGCHC 4 cut(s) 437, 472, 483, 785
SetI ASST 9 cut(s) 36, 62, 72, 389, 419, 557, 599, 612, 819
SfaNI GCATC 1 cut(s) 178
SinI GGWCC 2 cut(s) 173, 410
SmiMI CAYNNNNRTG 1 cut(s) 426
Sse9I AATT 6 cut(s) 88, 229, 703, 731, 824, 838
SsiI CCGC 1 cut(s) 141
StyD4I CCNGG 5 cut(s) 169, 207, 406, 446, 689
StyI CCWWGG 2 cut(s) 130, 499
TaaI ACNGT 3 cut(s) 325, 673, 700
TaiI ACGT 1 cut(s) 62
TaqI TCGA 1 cut(s) 751
TasI AATT 6 cut(s) 88, 229, 703, 731, 824, 838
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 646
Tru1I TTAA 3 cut(s) 485, 603, 831
Tru9I TTAA 3 cut(s) 485, 603, 831
TscAI CASTG 2 cut(s) 678, 774
TseI GCWGC 1 cut(s) 607
TspDTI ATGAA 1 cut(s) 525
TspGWI ACGGA 1 cut(s) 849
TspRI CASTG 2 cut(s) 678, 774
VneI GTGCAC 1 cut(s) 468
VpaK11BI GGWCC 2 cut(s) 173, 410
XapI RAATTY 3 cut(s) 703, 824, 838
XmiI GTMKAC 1 cut(s) 695
ZraI GACGTC 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.