RchiOBHm_Chr5g0067241

phosphatidylinositol ceramide inositolphosphotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006031
Physical Location & Seq
Forward (+)
73435859 .. 73439666
3808 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34297

Sequence Viewer

Length: 876 bp
ATGCCGTTTTACTTTGCTCGTGAGGCTCCTAAGTTGTGGAGGAAAATATGCAACGAGTCGTCAACTGAAGCTTCGCTTCTTGCTGGGAATTGGAAGTTTGTTCTTGCTGGTCTCATTTGTCAGTATCTTCATGGACTGGCTGTGCACGGAGTTCATTATCTACATCGGCCAGGACCAATACTTCAGGATGCTGGCTTCTTTTTACTCCCGGAACTTGGACAAGACAAAACTTATCTGAGTGAGACTCTGTTTGCTGTTGTATTTTTTTCTTTTATCTTGTGGACATTTCACCCGTTTGTTTTTCAGAGCAAAAAAATCTATACAGTTCTGATATGGTGCAGGGTACTTGCTTATTTAGCTGTGTCTCAGTTTCTCCGGATCATCACCTTCTACGCTACTCAGCTTCCTGGTCCAAACTATCATTGCCGCGAGGGCTCAGAACTTGCCAGGCTGCCCCCTCCAAAGAGTGCACTTGCAGTGCTCTTAATCAACTTTCCCCGAGGTGTTATGTATGGCTGTGGTGATTTGATTTTTTCATCACACATGATTTTCACTTTAGTATTTGTGCGCACATTTCAGAAATATGGAACAAGGAGGTGCATCAAGCAGTTTGCTTGGTTGGTTTCTGTGATTCTAAGTGTCTTGATTATAGCATCTCGTAAACATTACACCGTTGATGTTGTTGTTGCCTGGTACACTGTAAATTTGGTAGTTTCCTTTGTCGACCAAAAATTGCCAGAATTGCCTGATCGTTCCTTGGGTTCTACATCTCCACCGCTACTACCAACGAGTACCAAGGATAGGGACAGCAAAAACAGAGAAGAGCTTGAAAAATTCCGGAATGGAAATTCTTCTGACATCAGCACACGGGTCTGA

Protein Analysis

291

Amino Acids

33.22

Weight (kDa)

9.44

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP2_C PF14360 172 - 241 5.5e-23 PAP2 superfamily C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 569
AccI GTMKAC 1 cut(s) 723
AccII CGCG 1 cut(s) 429
AccIII TCCGGA 2 cut(s) 375, 837
AciI CCGC 2 cut(s) 427, 776
AclWI GGATC 1 cut(s) 386
AcoI YGGCCR 1 cut(s) 167
AcsI RAATTY 3 cut(s) 703, 833, 847
AcuI CTGAAG 2 cut(s) 87, 167
AfaI GTAC 3 cut(s) 345, 695, 793
AfiI CCNNNNNNNGG 2 cut(s) 215, 801
AgsI TTSAA 1 cut(s) 830
AjnI CCWGG 4 cut(s) 169, 406, 446, 689
AluBI AGCT 4 cut(s) 71, 359, 403, 826
AluI AGCT 4 cut(s) 71, 359, 403, 826
Alw21I GWGCWC 3 cut(s) 147, 472, 483
Alw26I GTCTC 3 cut(s) 116, 236, 369
Alw44I GTGCAC 2 cut(s) 143, 468
AlwI GGATC 1 cut(s) 386
Ama87I CYCGRG 1 cut(s) 498
Aor13HI TCCGGA 2 cut(s) 375, 837
AoxI GGCC 1 cut(s) 167
ApaLI GTGCAC 2 cut(s) 143, 468
ApeKI GCWGC 1 cut(s) 451
ApoI RAATTY 3 cut(s) 703, 833, 847
Asp700I GAANNNNTTC 1 cut(s) 850
AspLEI GCGC 1 cut(s) 570
AspS9I GGNCC 2 cut(s) 173, 410
AsuC2I CCSGG 1 cut(s) 209
AsuHPI GGTGA 3 cut(s) 281, 376, 533
AvaI CYCGRG 1 cut(s) 498
AvaII GGWCC 2 cut(s) 173, 410
BaeGI GKGCMC 2 cut(s) 147, 472
BanII GRGCYC 1 cut(s) 437
BauI CACGAG 1 cut(s) 18
Bbv12I GWGCWC 3 cut(s) 147, 472, 483
BbvI GCAGC 1 cut(s) 438
BciT130I CCWGG 4 cut(s) 171, 408, 448, 691
BcnI CCSGG 1 cut(s) 209
BcoDI GTCTC 3 cut(s) 116, 236, 369
BglI GCCNNNNNGGC 1 cut(s) 432
BisI GCNGC 2 cut(s) 427, 452
BlsI GCNGC 2 cut(s) 428, 453
Bme1390I CCNGG 5 cut(s) 171, 209, 408, 448, 691
Bme18I GGWCC 2 cut(s) 173, 410
BmeT110I CYCGRG 1 cut(s) 498
BmgT120I GGNCC 2 cut(s) 173, 410
BmiI GGNNCC 1 cut(s) 27
BmrFI CCNGG 5 cut(s) 171, 209, 408, 448, 691
BmsI GCATC 3 cut(s) 178, 609, 662
BplI GAGNNNNNCTC 2 cut(s) 229, 261
BpuMI CCSGG 1 cut(s) 209
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 3 cut(s) 499, 756, 795
BsaWI WCCGGW 2 cut(s) 375, 837
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 801
Bse1I ACTGG 1 cut(s) 141
Bse3DI GCAATG 1 cut(s) 421
BseAI TCCGGA 2 cut(s) 375, 837
BseBI CCWGG 4 cut(s) 171, 408, 448, 691
BseDI CCNNGG 3 cut(s) 499, 756, 795
BseGI GGATG 1 cut(s) 193
BseLI CCNNNNNNNGG 2 cut(s) 215, 801
BseMI GCAATG 1 cut(s) 421
BseMII CTCAG 4 cut(s) 227, 380, 413, 450
BseNI ACTGG 1 cut(s) 141
BseSI GKGCMC 2 cut(s) 147, 472
BseXI GCAGC 1 cut(s) 438
BseYI CCCAGC 1 cut(s) 83
BsgI GTGCAG 1 cut(s) 358
Bsh1236I CGCG 1 cut(s) 429
BshFI GGCC 1 cut(s) 169
BsiHKAI GWGCWC 3 cut(s) 147, 472, 483
BsiHKCI CYCGRG 1 cut(s) 498
BsiSI CCGG 3 cut(s) 209, 376, 838
BslFI GGGAC 1 cut(s) 818
BslI CCNNNNNNNGG 2 cut(s) 215, 801
BsmAI GTCTC 3 cut(s) 116, 236, 369
BsmFI GGGAC 1 cut(s) 818
BsnI GGCC 1 cut(s) 169
Bso31I GGTCTC 1 cut(s) 116
BsoBI CYCGRG 1 cut(s) 498
Bsp1286I GDGCHC 4 cut(s) 147, 437, 472, 483
Bsp13I TCCGGA 2 cut(s) 375, 837
Bsp143I GATC 2 cut(s) 378, 748
BspACI CCGC 2 cut(s) 427, 776
BspANI GGCC 1 cut(s) 169
BspCNI CTCAG 4 cut(s) 228, 379, 412, 449
BspEI TCCGGA 2 cut(s) 375, 837
BspFNI CGCG 1 cut(s) 429
BspLI GGNNCC 1 cut(s) 27
BspPI GGATC 1 cut(s) 386
BspQI GCTCTTC 1 cut(s) 816
BspTNI GGTCTC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 421
BsrI ACTGG 1 cut(s) 141
BssECI CCNNGG 3 cut(s) 499, 756, 795
BssMI GATC 2 cut(s) 378, 748
BssSI CACGAG 1 cut(s) 18
BssT1I CCWWGG 2 cut(s) 756, 795
Bst2BI CACGAG 1 cut(s) 18
Bst2UI CCWGG 4 cut(s) 171, 408, 448, 691
Bst4CI ACNGT 3 cut(s) 325, 673, 700
Bst6I CTCTTC 1 cut(s) 816
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 6 cut(s) 30, 236, 366, 399, 436, 635
BstF5I GGATG 1 cut(s) 193
BstFNI CGCG 1 cut(s) 429
BstHHI GCGC 1 cut(s) 570
BstKTI GATC 2 cut(s) 381, 751
BstMAI GTCTC 3 cut(s) 116, 236, 369
BstMBI GATC 2 cut(s) 378, 748
BstMWI GCNNNNNNNGC 4 cut(s) 23, 356, 432, 742
BstNI CCWGG 4 cut(s) 171, 408, 448, 691
BstSCI CCNGG 5 cut(s) 169, 207, 406, 446, 689
BstSLI GKGCMC 2 cut(s) 147, 472
BstUI CGCG 1 cut(s) 429
BstV1I GCAGC 1 cut(s) 438
BsuRI GGCC 1 cut(s) 169
BtsCI GGATG 1 cut(s) 193
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 2 cut(s) 483, 696
Cac8I GCNNGC 1 cut(s) 193
CfoI GCGC 1 cut(s) 570
Cfr13I GGNCC 2 cut(s) 173, 410
Csp6I GTAC 3 cut(s) 344, 694, 792
CviAII CATG 2 cut(s) 131, 544
CviQI GTAC 3 cut(s) 344, 694, 792
DdeI CTNAG 6 cut(s) 30, 236, 366, 399, 436, 635
DpnI GATC 2 cut(s) 380, 750
DpnII GATC 2 cut(s) 378, 748
EaeI YGGCCR 1 cut(s) 167
Eam1104I CTCTTC 1 cut(s) 816
EarI CTCTTC 1 cut(s) 816
Eco130I CCWWGG 2 cut(s) 756, 795
Eco24I GRGCYC 1 cut(s) 437
Eco31I GGTCTC 1 cut(s) 116
Eco47I GGWCC 2 cut(s) 173, 410
Eco57I CTGAAG 2 cut(s) 87, 167
Eco88I CYCGRG 1 cut(s) 498
EcoRII CCWGG 4 cut(s) 169, 406, 446, 689
EcoT14I CCWWGG 2 cut(s) 756, 795
EcoT38I GRGCYC 1 cut(s) 437
ErhI CCWWGG 2 cut(s) 756, 795
FaeI CATG 2 cut(s) 134, 547
FaiI YATR 9 cut(s) 49, 132, 321, 334, 509, 513, 545, 585, 650
FalI AAGNNNNNCTT 2 cut(s) 60, 92
FaqI GGGAC 1 cut(s) 818
FatI CATG 2 cut(s) 130, 543
FblI GTMKAC 1 cut(s) 723
Fnu4HI GCNGC 2 cut(s) 427, 452
FokI GGATG 1 cut(s) 200
FriOI GRGCYC 1 cut(s) 437
Fsp4HI GCNGC 2 cut(s) 427, 452
FspAI RTGCGCAY 1 cut(s) 569
FspI TGCGCA 1 cut(s) 569
GlaI GCGC 1 cut(s) 569
GluI GCNGC 2 cut(s) 427, 452
GsaI CCCAGC 1 cut(s) 87
HaeIII GGCC 1 cut(s) 169
HapII CCGG 3 cut(s) 209, 376, 838
HhaI GCGC 1 cut(s) 570
Hin1II CATG 2 cut(s) 134, 547
Hin6I GCGC 1 cut(s) 568
HinP1I GCGC 1 cut(s) 568
HincII GTYRAC 2 cut(s) 63, 724
HindII GTYRAC 2 cut(s) 63, 724
HindIII AAGCTT 1 cut(s) 69
HinfI GANTC 3 cut(s) 56, 244, 631
HpaII CCGG 3 cut(s) 209, 376, 838
HphI GGTGA 3 cut(s) 281, 376, 533
Hpy166II GTNNAC 7 cut(s) 63, 145, 282, 470, 662, 696, 724
Hpy188I TCNGA 7 cut(s) 237, 306, 330, 439, 579, 856, 875
Hpy188III TCNNGA 5 cut(s) 20, 185, 376, 643, 838
Hpy8I GTNNAC 7 cut(s) 63, 145, 282, 470, 662, 696, 724
HpyAV CCTTC 1 cut(s) 397
HpyCH4III ACNGT 3 cut(s) 325, 673, 700
HpyCH4V TGCA 6 cut(s) 51, 145, 339, 470, 476, 600
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 356, 432, 742
HpyF3I CTNAG 6 cut(s) 30, 236, 366, 399, 436, 635
Hsp92II CATG 2 cut(s) 134, 547
HspAI GCGC 1 cut(s) 568
Kpn2I TCCGGA 2 cut(s) 375, 837
Kzo9I GATC 2 cut(s) 378, 748
LguI GCTCTTC 1 cut(s) 816
LmnI GCTCC 1 cut(s) 31
Lsp1109I GCAGC 1 cut(s) 438
LweI GCATC 3 cut(s) 178, 609, 662
MalI GATC 2 cut(s) 380, 750
MboI GATC 2 cut(s) 378, 748
MboII GAAGA 3 cut(s) 119, 833, 843
MhlI GDGCHC 4 cut(s) 147, 437, 472, 483
MluCI AATT 6 cut(s) 88, 703, 731, 740, 833, 847
MlyI GAGTC 2 cut(s) 65, 238
MnlI CCTC 6 cut(s) 16, 33, 424, 468, 494, 588
MroI TCCGGA 2 cut(s) 375, 837
MroXI GAANNNNTTC 1 cut(s) 850
MseI TTAA 1 cut(s) 485
MspI CCGG 3 cut(s) 209, 376, 838
MspR9I CCNGG 5 cut(s) 171, 209, 408, 448, 691
MvaI CCWGG 4 cut(s) 171, 408, 448, 691
MvnI CGCG 1 cut(s) 429
MwoI GCNNNNNNNGC 4 cut(s) 23, 356, 432, 742
NciI CCSGG 1 cut(s) 209
NdeII GATC 2 cut(s) 378, 748
NlaIII CATG 2 cut(s) 134, 547
NlaIV GGNNCC 1 cut(s) 27
NsbI TGCGCA 1 cut(s) 569
PciSI GCTCTTC 1 cut(s) 816
PdmI GAANNNNTTC 1 cut(s) 850
PfeI GAWTC 1 cut(s) 631
PfoI TCCNGGA 1 cut(s) 207
PkrI GCNGC 2 cut(s) 428, 453
PleI GAGTC 2 cut(s) 64, 238
PpsI GAGTC 2 cut(s) 64, 238
Psp6I CCWGG 4 cut(s) 169, 406, 446, 689
PspFI CCCAGC 1 cut(s) 83
PspGI CCWGG 4 cut(s) 169, 406, 446, 689
PspN4I GGNNCC 1 cut(s) 27
PspPI GGNCC 2 cut(s) 173, 410
RsaI GTAC 3 cut(s) 345, 695, 793
RsaNI GTAC 3 cut(s) 344, 694, 792
SalI GTCGAC 1 cut(s) 722
SapI GCTCTTC 1 cut(s) 816
SaqAI TTAA 1 cut(s) 485
SatI GCNGC 2 cut(s) 427, 452
Sau3AI GATC 2 cut(s) 378, 748
Sau96I GGNCC 2 cut(s) 173, 410
SchI GAGTC 2 cut(s) 65, 238
ScrFI CCNGG 5 cut(s) 171, 209, 408, 448, 691
SduI GDGCHC 4 cut(s) 147, 437, 472, 483
SetI ASST 7 cut(s) 73, 361, 389, 405, 505, 599, 828
SfaNI GCATC 3 cut(s) 178, 609, 662
SinI GGWCC 2 cut(s) 173, 410
Sse9I AATT 6 cut(s) 88, 703, 731, 740, 833, 847
SsiI CCGC 2 cut(s) 427, 776
StyD4I CCNGG 5 cut(s) 169, 207, 406, 446, 689
StyI CCWWGG 2 cut(s) 756, 795
TaaI ACNGT 3 cut(s) 325, 673, 700
TaqI TCGA 1 cut(s) 723
TasI AATT 6 cut(s) 88, 703, 731, 740, 833, 847
TauI GCSGC 1 cut(s) 429
TfiI GAWTC 1 cut(s) 631
Tru1I TTAA 1 cut(s) 485
Tru9I TTAA 1 cut(s) 485
TscAI CASTG 2 cut(s) 483, 703
TseI GCWGC 1 cut(s) 451
TspDTI ATGAA 3 cut(s) 119, 143, 525
TspGWI ACGGA 1 cut(s) 162
TspRI CASTG 2 cut(s) 483, 703
VneI GTGCAC 2 cut(s) 143, 468
VpaK11BI GGWCC 2 cut(s) 173, 410
XapI RAATTY 3 cut(s) 703, 833, 847
XmiI GTMKAC 1 cut(s) 723
XmnI GAANNNNTTC 1 cut(s) 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.