Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0009672

Physical Location & Seq

Genomic Coordinates Reverse (-)

8069684 .. 8075203

Gene Structure 5520 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1095200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2577 bp

ATGCGCCCTTCCCACTTGCTTCCCAACAACACCAACACCCACAAACCTCAGACCCCAACTCCGCCACCACCGGCGGAGCTGCCTCCGCCTCACCCTTCCATTTCCATGGAGCTCGTACAGCCTTCGCACCCGCACCATGGCCCGGTGGCGGAATCCCTTTCTGGGTTCTCTTCTCCGGTGAGTTTCCGACGTGGGTTTGACCGGCCGGCGACTTCCTCCTCTCAGTCGCTTGAGATTTTTGAAGAAGACGGTGGTGGTGGTGGTGACAAGGTGCACGTGGCGGTTGGGAAGTCTGTGGAGAAAGCTTTGAGCTTGCTTCGTTGGACTTTCAAGCACTTTGGGTGTAAAGAAATTTGTCTTCTTCATGTTCATAAGCCTTCTCAGCACATTCCAACTCTCTTAGGAAAACTGCCCGCAGGTCAGGCAAATGCCGAGGTGGTGTCGGCTTTCAGGAGGGAGGAGAAGGAGCGGAAAATGAAGCTGTTGCAAAATTATTTGAGGGCTTGCTCTATTGCAAAGGTTAAAGCAAGCATTGTTATGGTTGAAGCTGATGAAATTCAGAAAGGAATTGTAGAGTTGGTCAACAAAAATGGTGTTAGGAAGCTTGTTATGGGAGCTGTGCCAGAAAATTGCCTGAAGGTGAAAAAGAGCTCCAGCAAGGCAAATTATGCAGCCAAATGTGCTCCATTGTTCTGTGAAATATGGTTTATCAACAAAGGGAAACATGTCTGGACGAGAGATGCTTCAGAAGGCCAAAGTTTTCCAACCTCATGTAGGCAACTGCAGGTTGCATCTGCAGAAAGTTTGAGGTCTGGATCGTTTGAAAATAGAGAGAATACGAACCTCCGTGAAGCATCGCCAACTTTAGCTTGTCCTACAGATACGTGCTTACCCTACAATATTCATACTTCAACTAGTACAAGTACTAGCTCTGGTTCTGGATATATTTCTGCTGAGGTAAGGATGTCCTCAGATTCTGATTTAACCGTTGAGGAACAGAGCTTATATCGTATGCTTACGGAAGCTACAACAGAAGCTGAGACCTCAAAGGACAAGGCATTTGCAGAGCTATCGAAGTGCAAAAGTTTAGAATCAGAAGCTATGAATGCTATAAACAAGGCCAAGGCATTTGAGTTAGCCCGTGCCCGAGAAGTTAAGCTTAGAAAAGAGGCTGGGGATGCCCTGAGAACCACGATCGAGGAAGAACAAAAACTCTTGGAAGAGAAGGAAGAAATTAGTGGTGAGATAAGGAGGGCCATGAGAAATATTGCCCTTCTAGACGTCCGAGCACAGGAAGCAAGTCGAAGGGTTGATGAAGTTTCTGGGGAAGTGAAGCAAATTCAGGCTTCGATTGCAACTCTTTGGCAGGAAAAACAGGGTATTCAAAGACAGAAGATGGAAGCCCTTCGCTGGATTGAGCGGTGGAGAAATCATCGACAAGCTGGAGCCGCAAACTGCAACGGGCTCATTGGGTTTGTTGAAGAACTGCCCAAGTTAGCGGAATTCTCATTGTCAGATTTGCAGACTGCAACATGCAATTTTTCTGAGAGCTTTAAAATCGGGCAGGGAGGATATGGTTGTGTTTATAAGGGGGAAATGCTGGGCAGAACTGTTGCCATAAGGAAGTTGCATCCACATAACATGCAAGAACAAGCCGAGTTTCAGCAAGAGGTGCAAGTTCTTGGAAAATTACAGCATCGTCATCTTGTGACCTTGCTTGGTGTGTGTCCAGAAGCATGGTCGCTTGTATACGAGTACTTATCAAACGGGAGCCTGCAAGACCATCTTTTTAGGAAAAGCAATATATCTCCAATGACATGGAAGACCCGAACACGGATAATTGCTGAAATCTCTAGTGCCCTTTGCTTCTTGCACTCATCTAAACCTGAGAAGATTGTCCATGGTGATTTGAAGCCGCAAAACATACTACTAGATTCTGAACTCAGATGCAAGATCTGTGATTTCGGGATTTGCAGGCTGGTAACTGAAGACAATCTTTACACACGAAGTTTCCGCCGGGGGGCTGAGCCAAAGGGTGCTTTTCCTTACACAGATCCAGAGGTGCAGAGAATCGGAGTCCTGACGCACAAGTCTGATATTTATTCCTTTGGGTTGATCATTCTGCAGTTAGTCACCAGACGGCCGCCAGTTGGCTTAGCTGGTGAGGTGCGCAAGGCAGTTTTAGGTGGAAACTTGGCATCAATCCTGGATTCTTCCGCTGGAGAATGGCCTATATCTGTGGCAACACGATTGGTAGATCTTGGTTTGCAATGCTGTGAATTGAATAGTAGGGAAAGACCAGAGCTTACACCAGCTTTAGTGAGGGAATTGGAGCAGTTGCATGCCTCGGAAGAGCGACCAGTGCCATCTTTTTTCTTGTGTCCGATCCTTCAGGAAATAATGCATGACCCTCAGTTGGCAGCTGATGGCTTCACTTACGAAGGTGAAGCTTTGCGTGGATGGTTACAAAATTGTCGCGAAACTTCTCCAATGACCAATTTGAAATTGAGTCACTTGCATCTCACTCCTAACCACGCCCTACGGCTTGCTATTCAAGATTGGCTTTGCAAGACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr U-box Usp

Protein Analysis

859

Amino Acids

95.61

Weight (kDa)

8.21

Isoelectric Point (pI)

54.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	517 - 772	2e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	519 - 770	5.2e-40	Protein tyrosine and serine/threonine kinase
U-box	PF04564	788 - 857	6.8e-17	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009672)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g35970 FvH4_3g35970 FvH4_3g35970 FvH4_3g35970 FvH4_3g35970
malus_domestica	MD03G1095200.v1.1 MD11G1110200.v1.1
prunus_persica	Prupe.6G078700_v2.0.a1 Prupe.6G078700_v2.0.a1
pyrus_communis	pycom03g07770 pycom11g09100
rosa_chinensis	RchiOBHm_Chr5g0064331
rosa_laevigata	RLG00000035717
rosa_multiflora	Rmu_sc0000806.1_g000029
rosa_roxburghii	Rroxscaffold_1G00016670
rosa_rugosa	Rorug05G0364100 Rorug05G0364100
rosa_samantha	Rh5AG422200 Rh5BG438100 Rh5CG460600 Rh5DG451000
rosa_wichuraiana	Rw5G039740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1587
AasI	GACNNNNNNGTC	1 cut(s)	2089
AatII	GACGTC	1 cut(s)	1284
Acc16I	TGCGCA	1 cut(s)	2171
Acc36I	ACCTGC	2 cut(s)	407, 775
AccBSI	CCGCTC	2 cut(s)	469, 1420
AccI	GTMKAC	1 cut(s)	1749
AccII	CGCG	1 cut(s)	2478
AclWI	GGATC	3 cut(s)	823, 2048, 2380
AcoI	YGGCCR	2 cut(s)	203, 2141
AcsI	RAATTY	4 cut(s)	351, 555, 1338, 1502
AcuI	CTGAAG	4 cut(s)	656, 729, 2007, 2375
AcvI	CACGTG	1 cut(s)	277
AcyI	GRCGYC	1 cut(s)	1281
AfaI	GTAC	4 cut(s)	117, 919, 925, 1757
AflIII	ACRYGT	1 cut(s)	724
AhlI	ACTAGT	1 cut(s)	914
AjiI	CACGTC	1 cut(s)	191
AjnI	CCWGG	1 cut(s)	2205
Alw21I	GWGCWC	5 cut(s)	114, 276, 653, 685, 1291
Alw26I	GTCTC	1 cut(s)	1034
Alw44I	GTGCAC	1 cut(s)	272
AlwI	GGATC	3 cut(s)	823, 2048, 2380
AlwNI	CAGNNNCTG	2 cut(s)	977, 1037
Ama87I	CYCGRG	1 cut(s)	1146
AoxI	GGCC	7 cut(s)	139, 203, 751, 1119, 1254, 2141, 2228
ApaLI	GTGCAC	1 cut(s)	272
ApeKI	GCWGC	3 cut(s)	79, 671, 2420
ApoI	RAATTY	4 cut(s)	351, 555, 1338, 1502
ArsI	GACNNNNNNTTYG	2 cut(s)	1043, 1075
Asp700I	GAANNNNTTC	1 cut(s)	1404
AspLEI	GCGC	2 cut(s)	6, 2172
AspS9I	GGNCC	2 cut(s)	140, 1254
AsuC2I	CCSGG	2 cut(s)	143, 2018
AsuHPI	GGTGA	9 cut(s)	83, 190, 275, 652, 1253, 1916, 2125, 2174, 2456
AvaI	CYCGRG	1 cut(s)	1146
BaeGI	GKGCMC	3 cut(s)	276, 1147, 1861
BanII	GRGCYC	3 cut(s)	114, 653, 1467
BbrPI	CACGTG	1 cut(s)	277
BbsI	GAAGAC	4 cut(s)	252, 350, 1829, 1995
Bbv12I	GWGCWC	5 cut(s)	114, 276, 653, 685, 1291
BbvCI	CCTCAGC	1 cut(s)	954
BbvI	GCAGC	3 cut(s)	66, 683, 2432
BccI	CCATC	5 cut(s)	1390, 1791, 2374, 2420, 2454
BceAI	ACGGC	2 cut(s)	2156, 2558
BcgI	CGANNNNNNTGC	2 cut(s)	1053, 1087
BciT130I	CCWGG	1 cut(s)	2207
BclI	TGATCA	1 cut(s)	2115
BcnI	CCSGG	2 cut(s)	143, 2018
BcoDI	GTCTC	1 cut(s)	1034
BcuI	ACTAGT	1 cut(s)	914
BfaI	CTAG	5 cut(s)	915, 927, 1277, 1854, 1931
BfmI	CTRYAG	4 cut(s)	782, 795, 876, 2123
BfuAI	ACCTGC	2 cut(s)	407, 775
BglII	AGATCT	2 cut(s)	1953, 2257
BisI	GCNGC	6 cut(s)	80, 672, 1449, 1916, 2144, 2421
BlpI	GCTNAGC	2 cut(s)	2025, 2155
BlsI	GCNGC	6 cut(s)	81, 673, 1450, 1917, 2145, 2422
BmcAI	AGTACT	2 cut(s)	925, 1757
Bme1390I	CCNGG	3 cut(s)	143, 2018, 2207
BmeT110I	CYCGRG	1 cut(s)	1146
BmgBI	CACGTC	1 cut(s)	191
BmgT120I	GGNCC	2 cut(s)	140, 1254
BmiI	GGNNCC	2 cut(s)	1447, 1772
BmrFI	CCNGG	3 cut(s)	143, 2018, 2207
BmsI	GCATC	9 cut(s)	730, 800, 863, 1168, 1639, 1705, 1937, 2207, 2527
BpiI	GAAGAC	4 cut(s)	252, 350, 1829, 1995
BpmI	CTGGAG	3 cut(s)	637, 1464, 2241
Bpu10I	CCTNAGC	1 cut(s)	954
Bpu1102I	GCTNAGC	2 cut(s)	2025, 2155
BpuEI	CTTGAG	1 cut(s)	251
BpuMI	CCSGG	2 cut(s)	143, 2018
BsaAI	YACGTR	2 cut(s)	277, 885
BsaHI	GRCGYC	1 cut(s)	1281
BsaI	GGTCTC	1 cut(s)	1034
BsaJI	CCNNGG	7 cut(s)	105, 136, 432, 1122, 1900, 2017, 2346
BsaWI	WCCGGW	1 cut(s)	175
BsaXI	ACNNNNNCTCC	2 cut(s)	43, 73
Bse118I	RCCGGY	3 cut(s)	70, 201, 205
Bse1I	ACTGG	2 cut(s)	2147, 2360
Bse3DI	GCAATG	1 cut(s)	2276
BseBI	CCWGG	1 cut(s)	2207
BseDI	CCNNGG	7 cut(s)	105, 136, 432, 1122, 1900, 2017, 2346
BseGI	GGATG	4 cut(s)	969, 1183, 1630, 2465
BseMI	GCAATG	1 cut(s)	2276
BseNI	ACTGG	2 cut(s)	2147, 2360
BseRI	GAGGAG	2 cut(s)	208, 473
BseSI	GKGCMC	3 cut(s)	276, 1147, 1861
BseX3I	CGGCCG	2 cut(s)	203, 2141
BseXI	GCAGC	3 cut(s)	66, 683, 2432
BseYI	CCCAGC	2 cut(s)	1172, 1600
BsgI	GTGCAG	1 cut(s)	2084
Bsh1236I	CGCG	1 cut(s)	2478
Bsh1285I	CGRYCG	3 cut(s)	206, 1197, 2144
BshFI	GGCC	7 cut(s)	141, 205, 753, 1121, 1256, 2143, 2230
BsiEI	CGRYCG	3 cut(s)	206, 1197, 2144
BsiHKAI	GWGCWC	5 cut(s)	114, 276, 653, 685, 1291
BsiHKCI	CYCGRG	1 cut(s)	1146
BsiSI	CCGG	6 cut(s)	71, 143, 176, 202, 206, 2017
BsmAI	GTCTC	1 cut(s)	1034
BsmI	GAATGC	1 cut(s)	1111
BsnI	GGCC	7 cut(s)	141, 205, 753, 1121, 1256, 2143, 2230
Bso31I	GGTCTC	1 cut(s)	1034
BsoBI	CYCGRG	1 cut(s)	1146
Bsp1286I	GDGCHC	8 cut(s)	114, 276, 653, 685, 1147, 1291, 1467, 1861
Bsp143I	GATC	7 cut(s)	815, 1194, 1953, 2053, 2115, 2257, 2385
Bsp1720I	GCTNAGC	2 cut(s)	2025, 2155
Bsp19I	CCATGG	3 cut(s)	105, 136, 1900
Bsp68I	TCGCGA	1 cut(s)	2478
BspANI	GGCC	7 cut(s)	141, 205, 753, 1121, 1256, 2143, 2230
BspFNI	CGCG	1 cut(s)	2478
BspLI	GGNNCC	2 cut(s)	1447, 1772
BspMAI	CTGCAG	3 cut(s)	786, 799, 2127
BspMI	ACCTGC	2 cut(s)	407, 775
BspPI	GGATC	3 cut(s)	823, 2048, 2380
BspQI	GCTCTTC	1 cut(s)	2346
BspTNI	GGTCTC	1 cut(s)	1034
BsrBI	CCGCTC	2 cut(s)	469, 1420
BsrDI	GCAATG	1 cut(s)	2276
BsrFI	RCCGGY	3 cut(s)	70, 201, 205
BsrI	ACTGG	2 cut(s)	2147, 2360
BssAI	RCCGGY	3 cut(s)	70, 201, 205
BssECI	CCNNGG	7 cut(s)	105, 136, 432, 1122, 1900, 2017, 2346
BssMI	GATC	7 cut(s)	815, 1194, 1953, 2053, 2115, 2257, 2385
BssNAI	GTATAC	1 cut(s)	1750
BssNI	GRCGYC	1 cut(s)	1281
BssT1I	CCWWGG	4 cut(s)	105, 136, 1122, 1900
Bst1107I	GTATAC	1 cut(s)	1750
Bst2UI	CCWGG	1 cut(s)	2207
Bst4CI	ACNGT	3 cut(s)	251, 988, 1612
Bst6I	CTCTTC	3 cut(s)	175, 1215, 2346
BstACI	GRCGYC	1 cut(s)	1281
BstAPI	GCANNNNNTGC	2 cut(s)	668, 1672
BstBAI	YACGTR	2 cut(s)	277, 885
BstC8I	GCNNGC	9 cut(s)	207, 314, 414, 505, 529, 1775, 1976, 2343, 2547
BstDSI	CCRYGG	3 cut(s)	105, 136, 1900
BstENI	CCTNNNNNAGG	1 cut(s)	772
BstF5I	GGATG	4 cut(s)	969, 1183, 1630, 2465
BstFNI	CGCG	1 cut(s)	2478
BstHHI	GCGC	2 cut(s)	6, 2172
BstKTI	GATC	7 cut(s)	818, 1197, 1956, 2056, 2118, 2260, 2388
BstMAI	GTCTC	1 cut(s)	1034
BstMBI	GATC	7 cut(s)	815, 1194, 1953, 2053, 2115, 2257, 2385
BstMCI	CGRYCG	3 cut(s)	206, 1197, 2144
BstNI	CCWGG	1 cut(s)	2207
BstNSI	RCATGY	4 cut(s)	728, 1536, 1645, 2345
BstSCI	CCNGG	3 cut(s)	141, 2016, 2205
BstSFI	CTRYAG	4 cut(s)	782, 795, 876, 2123
BstSLI	GKGCMC	3 cut(s)	276, 1147, 1861
BstUI	CGCG	1 cut(s)	2478
BstV1I	GCAGC	3 cut(s)	66, 683, 2432
BstV2I	GAAGAC	4 cut(s)	252, 350, 1829, 1995
BstX2I	RGATCY	3 cut(s)	1953, 2053, 2257
BstXI	CCANNNNNNTGG	3 cut(s)	106, 1737, 1818
BstYI	RGATCY	3 cut(s)	1953, 2053, 2257
BstZ17I	GTATAC	1 cut(s)	1750
BstZI	CGGCCG	2 cut(s)	203, 2141
BsuRI	GGCC	7 cut(s)	141, 205, 753, 1121, 1256, 2143, 2230
BtgI	CCRYGG	3 cut(s)	105, 136, 1900
BtgZI	GCGATG	1 cut(s)	840
BtrI	CACGTC	1 cut(s)	191
BtsCI	GGATG	4 cut(s)	969, 1183, 1630, 2465
BtsIMutI	CAGTG	1 cut(s)	2367
BtuMI	TCGCGA	1 cut(s)	2478
BveI	ACCTGC	2 cut(s)	407, 775
Cac8I	GCNNGC	9 cut(s)	207, 314, 414, 505, 529, 1775, 1976, 2343, 2547
CaiI	CAGNNNCTG	2 cut(s)	977, 1037
CfoI	GCGC	2 cut(s)	6, 2172
Cfr10I	RCCGGY	3 cut(s)	70, 201, 205
Cfr13I	GGNCC	2 cut(s)	140, 1254
CseI	GACGC	1 cut(s)	2092
Csp6I	GTAC	4 cut(s)	116, 918, 924, 1756
CviQI	GTAC	4 cut(s)	116, 918, 924, 1756
DpnI	GATC	7 cut(s)	817, 1196, 1955, 2055, 2117, 2259, 2387
DpnII	GATC	7 cut(s)	815, 1194, 1953, 2053, 2115, 2257, 2385
DraI	TTTAAA	1 cut(s)	1555
DrdI	GACNNNNNNGTC	1 cut(s)	2089
DseDI	GACNNNNNNGTC	1 cut(s)	2089
EaeI	YGGCCR	2 cut(s)	203, 2141
EagI	CGGCCG	2 cut(s)	203, 2141
Eam1104I	CTCTTC	3 cut(s)	175, 1215, 2346
EarI	CTCTTC	3 cut(s)	175, 1215, 2346
EciI	GGCGGA	5 cut(s)	51, 75, 89, 164, 2003
Ecl136II	GAGCTC	2 cut(s)	112, 651
EclXI	CGGCCG	2 cut(s)	203, 2141
Eco130I	CCWWGG	4 cut(s)	105, 136, 1122, 1900
Eco24I	GRGCYC	3 cut(s)	114, 653, 1467
Eco31I	GGTCTC	1 cut(s)	1034
Eco52I	CGGCCG	2 cut(s)	203, 2141
Eco53kI	GAGCTC	2 cut(s)	112, 651
Eco57I	CTGAAG	4 cut(s)	656, 729, 2007, 2375
Eco72I	CACGTG	1 cut(s)	277
Eco88I	CYCGRG	1 cut(s)	1146
EcoICRI	GAGCTC	2 cut(s)	112, 651
EcoNI	CCTNNNNNAGG	1 cut(s)	772
EcoRI	GAATTC	1 cut(s)	1502
EcoRII	CCWGG	1 cut(s)	2205
EcoT14I	CCWWGG	4 cut(s)	105, 136, 1122, 1900
EcoT22I	ATGCAT	1 cut(s)	2406
EcoT38I	GRGCYC	3 cut(s)	114, 653, 1467
ErhI	CCWWGG	4 cut(s)	105, 136, 1122, 1900
FalI	AAGNNNNNCTT	7 cut(s)	1143, 1175, 1770, 1802, 1980, 2012, 2547
FauI	CCCGC	2 cut(s)	138, 421
FbaI	TGATCA	1 cut(s)	2115
FblI	GTMKAC	1 cut(s)	1749
Fnu4HI	GCNGC	6 cut(s)	80, 672, 1449, 1916, 2144, 2421
FokI	GGATG	4 cut(s)	976, 1190, 1617, 2472
FriOI	GRGCYC	3 cut(s)	114, 653, 1467
Fsp4HI	GCNGC	6 cut(s)	80, 672, 1449, 1916, 2144, 2421
FspBI	CTAG	5 cut(s)	915, 927, 1277, 1854, 1931
FspI	TGCGCA	1 cut(s)	2171
GlaI	GCGC	2 cut(s)	5, 2171
GluI	GCNGC	6 cut(s)	80, 672, 1449, 1916, 2144, 2421
GsaI	CCCAGC	2 cut(s)	1176, 1604
GsuI	CTGGAG	3 cut(s)	637, 1464, 2241
HaeIII	GGCC	7 cut(s)	141, 205, 753, 1121, 1256, 2143, 2230
HapII	CCGG	6 cut(s)	71, 143, 176, 202, 206, 2017
HgaI	GACGC	1 cut(s)	2092
HhaI	GCGC	2 cut(s)	6, 2172
Hin1I	GRCGYC	1 cut(s)	1281
Hin6I	GCGC	2 cut(s)	4, 2170
HinP1I	GCGC	2 cut(s)	4, 2170
HincII	GTYRAC	1 cut(s)	583
HindII	GTYRAC	1 cut(s)	583
HindIII	AAGCTT	4 cut(s)	303, 602, 1157, 2448
HinfI	GANTC	8 cut(s)	152, 974, 1091, 1934, 2070, 2076, 2210, 2509
HpaII	CCGG	6 cut(s)	71, 143, 176, 202, 206, 2017
HphI	GGTGA	9 cut(s)	83, 190, 275, 652, 1253, 1916, 2125, 2174, 2456
Hpy166II	GTNNAC	3 cut(s)	274, 583, 1750
Hpy8I	GTNNAC	3 cut(s)	274, 583, 1750
Hpy99I	CGWCG	1 cut(s)	192
HpyCH4III	ACNGT	3 cut(s)	251, 988, 1612
HpyCH4IV	ACGT	4 cut(s)	190, 276, 884, 1281
HpySE526I	ACGT	4 cut(s)	190, 276, 884, 1281
Hsp92I	GRCGYC	1 cut(s)	1281
HspAI	GCGC	2 cut(s)	4, 2170
KroI	GCCGGC	1 cut(s)	205
KroNI	GCCGGC	1 cut(s)	207
Ksp22I	TGATCA	1 cut(s)	2115
Kzo9I	GATC	7 cut(s)	815, 1194, 1953, 2053, 2115, 2257, 2385
LguI	GCTCTTC	1 cut(s)	2346
LmnI	GCTCC	9 cut(s)	76, 109, 466, 614, 656, 688, 1445, 1770, 2332
Lsp1109I	GCAGC	3 cut(s)	66, 683, 2432
LweI	GCATC	9 cut(s)	730, 800, 863, 1168, 1639, 1705, 1937, 2207, 2527
MaeI	CTAG	5 cut(s)	915, 927, 1277, 1854, 1931
MaeII	ACGT	4 cut(s)	190, 276, 884, 1281
MaeIII	GTNAC	6 cut(s)	263, 1708, 1981, 2131, 2463, 2510
MalI	GATC	7 cut(s)	817, 1196, 1955, 2055, 2117, 2259, 2387
MbiI	CCGCTC	2 cut(s)	469, 1420
MboI	GATC	7 cut(s)	815, 1194, 1953, 2053, 2115, 2257, 2385
MflI	RGATCY	3 cut(s)	1953, 2053, 2257
MhlI	GDGCHC	8 cut(s)	114, 276, 653, 685, 1147, 1291, 1467, 1861
MlyI	GAGTC	2 cut(s)	2085, 2518
MmeI	TCCRAC	4 cut(s)	211, 302, 416, 788
Mph1103I	ATGCAT	1 cut(s)	2406
MroNI	GCCGGC	1 cut(s)	205
MroXI	GAANNNNTTC	1 cut(s)	1404
MseI	TTAA	4 cut(s)	522, 983, 1155, 1554
MslI	CAYNNNNRTG	2 cut(s)	104, 536
MspA1I	CMGCKG	2 cut(s)	2219, 2423
MspI	CCGG	6 cut(s)	71, 143, 176, 202, 206, 2017
MspR9I	CCNGG	3 cut(s)	143, 2018, 2207
Mva1269I	GAATGC	1 cut(s)	1111
MvaI	CCWGG	1 cut(s)	2207
MvnI	CGCG	1 cut(s)	2478
NaeI	GCCGGC	1 cut(s)	207
NciI	CCSGG	2 cut(s)	143, 2018
NcoI	CCATGG	3 cut(s)	105, 136, 1900
NdeII	GATC	7 cut(s)	815, 1194, 1953, 2053, 2115, 2257, 2385
NgoMIV	GCCGGC	1 cut(s)	205
NlaIV	GGNNCC	2 cut(s)	1447, 1772
NmeAIII	GCCGAG	2 cut(s)	457, 1681
NmuCI	GTSAC	4 cut(s)	263, 1708, 2131, 2510
NruI	TCGCGA	1 cut(s)	2478
NsbI	TGCGCA	1 cut(s)	2171
NsiI	ATGCAT	1 cut(s)	2406
NspI	RCATGY	4 cut(s)	728, 1536, 1645, 2345
PaeI	GCATGC	1 cut(s)	2345
PciI	ACATGT	1 cut(s)	724
PciSI	GCTCTTC	1 cut(s)	2346
PctI	GAATGC	1 cut(s)	1111
PdiI	GCCGGC	1 cut(s)	207
PdmI	GAANNNNTTC	1 cut(s)	1404
PfeI	GAWTC	6 cut(s)	152, 974, 1091, 1934, 2070, 2210
PfoI	TCCNGGA	1 cut(s)	2205
PkrI	GCNGC	6 cut(s)	81, 673, 1450, 1917, 2145, 2422
Ple19I	CGATCG	1 cut(s)	1197
PleI	GAGTC	2 cut(s)	2084, 2517
PmaCI	CACGTG	1 cut(s)	277
PmlI	CACGTG	1 cut(s)	277
PpsI	GAGTC	2 cut(s)	2084, 2517
Ppu21I	YACGTR	2 cut(s)	277, 885
PscI	ACATGT	1 cut(s)	724
PsiI	TTATAA	1 cut(s)	1587
Psp124BI	GAGCTC	2 cut(s)	114, 653
Psp6I	CCWGG	1 cut(s)	2205
PspCI	CACGTG	1 cut(s)	277
PspFI	CCCAGC	2 cut(s)	1172, 1600
PspGI	CCWGG	1 cut(s)	2205
PspN4I	GGNNCC	2 cut(s)	1447, 1772
PspPI	GGNCC	2 cut(s)	140, 1254
PstI	CTGCAG	3 cut(s)	786, 799, 2127
PstNI	CAGNNNCTG	2 cut(s)	977, 1037
PsuI	RGATCY	3 cut(s)	1953, 2053, 2257
PvuI	CGATCG	1 cut(s)	1197
PvuII	CAGCTG	1 cut(s)	2423
RruI	TCGCGA	1 cut(s)	2478
RsaI	GTAC	4 cut(s)	117, 919, 925, 1757
RsaNI	GTAC	4 cut(s)	116, 918, 924, 1756
RseI	CAYNNNNRTG	2 cut(s)	104, 536
SacI	GAGCTC	2 cut(s)	114, 653
SapI	GCTCTTC	1 cut(s)	2346
SaqAI	TTAA	4 cut(s)	522, 983, 1155, 1554
SatI	GCNGC	6 cut(s)	80, 672, 1449, 1916, 2144, 2421
Sau3AI	GATC	7 cut(s)	815, 1194, 1953, 2053, 2115, 2257, 2385
Sau96I	GGNCC	2 cut(s)	140, 1254
ScaI	AGTACT	2 cut(s)	925, 1757
SchI	GAGTC	2 cut(s)	2085, 2518
ScrFI	CCNGG	3 cut(s)	143, 2018, 2207
SduI	GDGCHC	8 cut(s)	114, 276, 653, 685, 1147, 1291, 1467, 1861
SfaNI	GCATC	9 cut(s)	730, 800, 863, 1168, 1639, 1705, 1937, 2207, 2527
SfcI	CTRYAG	4 cut(s)	782, 795, 876, 2123
SgrAI	CRCCGGYG	1 cut(s)	70
SmiMI	CAYNNNNRTG	2 cut(s)	104, 536
SmlI	CTYRAG	1 cut(s)	230
SmoI	CTYRAG	1 cut(s)	230
SpeI	ACTAGT	1 cut(s)	914
SphI	GCATGC	1 cut(s)	2345
SspI	AATATT	2 cut(s)	901, 1267
SspMI	CTAG	5 cut(s)	915, 927, 1277, 1854, 1931
SstI	GAGCTC	2 cut(s)	114, 653
StyD4I	CCNGG	3 cut(s)	141, 2016, 2205
StyI	CCWWGG	4 cut(s)	105, 136, 1122, 1900
TaaI	ACNGT	3 cut(s)	251, 988, 1612
TaiI	ACGT	4 cut(s)	193, 279, 887, 1284
TaqI	TCGA	5 cut(s)	1073, 1197, 1303, 1349, 1435
TatI	WGTACW	3 cut(s)	917, 923, 1755
TauI	GCSGC	3 cut(s)	1451, 1918, 2146
TfiI	GAWTC	6 cut(s)	152, 974, 1091, 1934, 2070, 2210
Tru1I	TTAA	4 cut(s)	522, 983, 1155, 1554
Tru9I	TTAA	4 cut(s)	522, 983, 1155, 1554
TscAI	CASTG	1 cut(s)	2367
TseFI	GTSAC	4 cut(s)	263, 1708, 2131, 2510
TseI	GCWGC	3 cut(s)	79, 671, 2420
Tsp45I	GTSAC	4 cut(s)	263, 1708, 2131, 2510
TspDTI	ATGAA	7 cut(s)	353, 359, 491, 567, 893, 1118, 1329
TspGWI	ACGGA	3 cut(s)	836, 1034, 1849
TspRI	CASTG	1 cut(s)	2367
VneI	GTGCAC	1 cut(s)	272
XagI	CCTNNNNNAGG	1 cut(s)	772
XapI	RAATTY	4 cut(s)	351, 555, 1338, 1502
XbaI	TCTAGA	1 cut(s)	1276
XceI	RCATGY	4 cut(s)	728, 1536, 1645, 2345
XmiI	GTMKAC	1 cut(s)	1749
XmnI	GAANNNNTTC	1 cut(s)	1404
XspI	CTAG	5 cut(s)	915, 927, 1277, 1854, 1931
ZraI	GACGTC	1 cut(s)	1282
ZrmI	AGTACT	2 cut(s)	925, 1757
Zsp2I	ATGCAT	1 cut(s)	2406

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.