Rorug05G0364100

U-box domain-containing protein 33-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000536
Physical Location & Seq
Reverse (-)
48348267 .. 48356821
8555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0364100.1

Sequence Viewer

Length: 2223 bp
ATGGGCACCCAAAGGCCAATTCTGCAAGTGAGAACCCTCACGACCTTCACAAGACACCCAGTGTTTCACTCTCAGAGATCCCATTCTATATCTTCTTGCCCATCAAACCGCTACTTGGAAAATGGTGTTACACTCGTGCAGTCCAAGCGTACCAGCCAAGAACCCTTTTTATCAGCTTCTCTGTCAGATTCTCTTTTGGTAGAGAAGCTTTTGTTGGGTTTGAAGCAAGGTAATCTCAATTATCTGCGTAACTATCTGTTTCGGTTAAACCCACTTCTTGTTGTTGAACTTGTTTCCCGTTGCCGTGAAAATCTGCATCTGGGTCTGAAATTCGTTGACTTGATTATGTTAAATTGTCCGAATTTCAAGCACTCGTCACAGTCTTTGAGTGCAATGATTCACATTTTGGTCAGGGCCCGAAGGGCTTCGGATGCTCAAGCTTTAATGCTTAGAATGGTTAGGAAGAGTGGCGTCTCCCGTGTTGAAATAGTTGAGTCTTTGGTTTTGACCTGTAGCAGTTGTGGCTCTAGCTGCTTCGTTTTTGACTTGTTGATTAGGACGTATGTGCAAGCTAGGAAGTTGAGAGAAGGGTTTGAGGCGTTTAAGGCACTTAGAAGCAAGGGAGTTGCTGTTTCGATAAATGCTGCTAATAGTCTTCTTGGTGGGCTTGTGAAGGTAGGGTGGGTAGATTTGGCATGGGAAGTGTATGGGGAAGTTGTTAGCAGCGGGGTTCAGTTAAATGTTTATACACTAAACATTATGGTTAATGCCTTGTGTAAAGATGGTAAAATCGATAGGGTTAAATTGTTCATATCGGACATGGCTGAGAAGGGGGTTTGTACAGATATTGTGACATATAATACTCTAATCAATGCCTATTGTCGTGAAGGGCTTGTGGAAGAAGCTTTCCAGTTGAAAAACTCTATGGCTTCTAAGGGTTTGAGACCAGAGCTTTTCACATACAATGCTATCATAAATGGATTATGTAGGGTAGGAAATTATGCGAGGGCAAAGGAAGTTTTGTATGAGATGTTGCAGAATGGATTAAGTCCTGATACTACTACCTTTAATACACTGCTGGTTGAGAGTTGTAGAAAAGATAATATTTCAGAGGCTGAAGGAATTTTTTGTGAAATGTCATGTAGAGGTGTTGTTCCTGATTTAGTCAGCTTCAGTTCAATAATTGGGGTGCTTTTGAGGAATGGACATTGTGATCATGCACTATTATATTTTCAAGAAATGAAAACTGCAGGCTTGGTTCCAGATAATGTGATCTATACTATCCTTATAGACGGATATTGTAGAAATGGCAAAATGTCAGAGGCGTTGAAGTTGCGGGATGAAATGCTTGAGCAAGGCTGCGTGGTGGATGTTATTACCTTCAATACTATTTTAAATGGACTTTGTCGGGAGAAGATGCTTGCTGATGCAGAAAAACTCTTCAATGAGATGGTGGAAAGGGGTGTCTTTCCAGATTTTTATACTTTCACAACACTTATTCATGGTTATTGTAAAAACGGGAATATGGCTAAATCCTTAAGTTTGTTCGAGGCAATGACTTGTAAAAATATCAAGCCTGATATTGTCACGTACAACACATTGATTGATGGATTCTGCAAAGTAGGTGACATGGATAAAGCTAAGGAGCTATGGAGTGACATGGTTTCTAGAAGAATGTTCCCTAATTACATTTCATATGGCATTCTTATCAATGGATTCTGTAGCTTGGGACTTGTCCATGAGGCGCTTCGTTTGTGGGACCAGATGATTGAAGAAGGTATCAAACCCACTCTAGTGACTTGTAATACTGTCATAAAGGGCTATAGCCGCTCTGGTGACACAGAAAAGGCATATGAGTTCTTAGGCAAAATGATTTCAAAAGGAATTGTTCCTGATAGTATTACTTACAACACTCTGATTAATGGATATGTAAAAGAAGAAAACCTGGATAAAGCCTTTTTTTCAGTGAAGGAGATGGAAAAGCAAGGACTACTGCCAGATGTGATTACATACAATGTCATTCTATATGGGTTCTGTAGACAGGGTAGACTGCATGAAGCTGAGTTAGTTTTGCGAAAGATGATTGAGAAAGGTGTAAATCCTGATAGATCCACTTATACGTCATTAATAAATGGACATGTGACCCAGGACAACTTGAGAGAGGCATTCCGCTACCATGATGAAATGCTGCAGAGGGGATTTGTACCAGATGATGAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr U-box Usp

Protein Analysis

740

Amino Acids

83.37

Weight (kDa)

7.91

Isoelectric Point (pI)

33.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 215 - 260 7.5e-10 PPR repeat family
PPR_long PF17177 232 - 352 1.4e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 237 - 293 7.5e-06 Pentatricopeptide repeat domain
PPR_3 PF13812 272 - 326 9.4e-11 Pentatricopeptide repeat domain
TPR_24 PF23276 276 - 376 7.3e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 277 - 309 3.3e-13 PPR repeat
PPR_2 PF13041 282 - 330 1e-18 PPR repeat family
PPR PF01535 284 - 314 5.3e-09 PPR repeat
PPR_3 PF13812 305 - 360 4.8e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 312 - 344 2.6e-09 PPR repeat
PPR_2 PF13041 317 - 364 5.2e-12 PPR repeat family
PPR PF01535 319 - 349 6.7e-07 PPR repeat
PPR_3 PF13812 340 - 398 2.6e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 351 - 396 3.8e-09 PPR repeat family
PPR_2 PF13041 386 - 432 1.5e-08 PPR repeat family
PPR_3 PF13812 411 - 466 2.5e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 418 - 449 3.5e-13 PPR repeat
PPR_2 PF13041 421 - 470 2.9e-18 PPR repeat family
PPR PF01535 424 - 454 2.9e-10 PPR repeat
PPR_long PF17177 435 - 563 3.9e-10 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 453 - 485 1.8e-12 PPR repeat
PPR_2 PF13041 457 - 505 1.3e-19 PPR repeat family
PPR PF01535 459 - 489 6.4e-08 PPR repeat
PPR_1 PF12854 487 - 519 3.3e-11 PPR repeat
PPR PF01535 494 - 524 2.9e-07 PPR repeat
PPR_2 PF13041 495 - 540 4.8e-17 PPR repeat family
PPR_3 PF13812 514 - 572 4.4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 522 - 555 1.2e-14 PPR repeat
PPR_2 PF13041 526 - 574 1.1e-18 PPR repeat family
PPR PF01535 529 - 558 1.9e-09 PPR repeat
PPR_1 PF12854 559 - 589 1.8e-09 PPR repeat
PPR_2 PF13041 561 - 600 8.7e-09 PPR repeat family
PPR PF01535 564 - 594 9.4e-08 PPR repeat
PPR_long PF17177 580 - 716 6e-13 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 585 - 645 2.1e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 593 - 624 2.6e-06 PPR repeat
PPR_2 PF13041 596 - 643 2.6e-15 PPR repeat family
PPR PF01535 599 - 629 9.6e-08 PPR repeat
PPR_1 PF12854 627 - 660 8.5e-09 PPR repeat
PPR_2 PF13041 631 - 680 2.6e-16 PPR repeat family
PPR PF01535 634 - 664 4.8e-06 PPR repeat
PPR_3 PF13812 656 - 711 2e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 663 - 694 7.3e-12 PPR repeat
PPR_2 PF13041 666 - 713 6.1e-15 PPR repeat family
PPR PF01535 669 - 699 4.8e-07 PPR repeat
PPR_2 PF13041 705 - 738 4.8e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000536)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37620 AT2G37620 AT2G37620 AT2G37620 AT3G46520 AT3G46520 AT3G53750 AT3G53750 AT5G59370 AT5G59370
fragaria_vesca FvH4_3g35960 FvH4_6g13940 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_7g22410 FvH4_7g22410 FvH4_7g22410
malus_domestica MD03G1095300.v1.1 MD04G1127400.v1.1 MD11G1110300.v1.1 MD12G1056900.v1.1 MD12G1140800.v1.1 MD14G1056700.v1.1
prunus_persica Prupe.6G078800_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.7G077300_v2.0.a1 Prupe.7G077300_v2.0.a1
pyrus_communis pycom01g02640 pycom03g07780 pycom11g09110 pycom126g00040 pycom14g04650 pycom15g30330
rosa_chinensis RchiOBHm_Chr1g0367041 RchiOBHm_Chr3g0466761 RchiOBHm_Chr3g0479651 RchiOBHm_Chr5g0064311
rosa_laevigata RLG00000023550 RLG00000024555 RLG00000027304 RLG00000035715
rosa_multiflora Rmu_co8518979.1_g000001 Rmu_sc0002269.1_g000004 Rmu_sc0003467.1_g000022 Rmu_sc0003755.1_g000002 Rmu_sc0013440.1_g000001 Rmu_sc0013700.1_g000002 Rmu_sc0026659.1_g000001 Rmu_sc0037142.1_g000001
rosa_roxburghii Rroxscaffold_1G00016700 Rroxscaffold_4G00289680 Rroxscaffold_6G00402090
rosa_rugosa Rorug01G0334100 Rorug03G0083700 Rorug03G0083800 Rorug03G0177100 Rorug03G0177200 Rorug05G0364000 Rorug05G0364100
rosa_samantha Rh1AG340900 Rh1BG301400 Rh1CG317600 Rh1DG333800 Rh2AG276500 Rh3AG130800 Rh3AG227900 Rh3BG151800 Rh3BG261100 Rh3CG151700 Rh3DG152500 Rh3DG254400 Rh5AG421900 Rh5BG437600 Rh5CG460300 Rh5DG450700
rosa_wichuraiana Rw0G023870 Rw1G030280 Rw3G012360 Rw3G020390 Rw5G039710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 5
AccBSI CCGCTC 1 cut(s) 1830
AccI GTMKAC 2 cut(s) 2038, 2047
AciI CCGC 5 cut(s) 109, 726, 1336, 1828, 2170
AclWI GGATC 2 cut(s) 72, 2103
AcsI RAATTY 4 cut(s) 329, 361, 1122, 2216
AcuI CTGAAG 2 cut(s) 1137, 1156
AcyI GRCGYC 1 cut(s) 471
AdeI CACNNNGTG 1 cut(s) 61
AfaI GTAC 4 cut(s) 151, 841, 1592, 2205
AfiI CCNNNNNNNGG 1 cut(s) 115
AflII CTTAAG 1 cut(s) 1537
AflIII ACRYGT 1 cut(s) 2137
AjnI CCWGG 2 cut(s) 1944, 2145
AjuI GAANNNNNNNTTGG 3 cut(s) 35, 197, 229
AleI CACNNNNGTG 1 cut(s) 1793
Alw26I GTCTC 2 cut(s) 478, 937
AlwI GGATC 2 cut(s) 72, 2103
AlwNI CAGNNNCTG 1 cut(s) 1115
AoxI GGCC 2 cut(s) 14, 414
ApaI GGGCCC 1 cut(s) 418
ApeKI GCWGC 5 cut(s) 531, 644, 723, 1359, 2188
ApoI RAATTY 4 cut(s) 329, 361, 1122, 2216
AseI ATTAAT 2 cut(s) 1920, 2126
Asp700I GAANNNNTTC 1 cut(s) 424
AspLEI GCGC 1 cut(s) 1747
AspS9I GGNCC 3 cut(s) 414, 415, 1759
AsuHPI GGTGA 2 cut(s) 1637, 1847
AvaII GGWCC 1 cut(s) 1759
BaeGI GKGCMC 2 cut(s) 8, 418
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 418
BauI CACGAG 1 cut(s) 134
BbsI GAAGAC 1 cut(s) 647
BbvI GCAGC 5 cut(s) 518, 631, 735, 1346, 2175
BccI CCATC 5 cut(s) 109, 776, 1444, 1601, 1969
BceAI ACGGC 1 cut(s) 288
BciT130I CCWGG 2 cut(s) 1946, 2147
BclI TGATCA 1 cut(s) 1213
BcoDI GTCTC 2 cut(s) 478, 937
BfaI CTAG 5 cut(s) 528, 573, 1668, 1793, 2221
BfmI CTRYAG 6 cut(s) 511, 1248, 1720, 1822, 2035, 2189
BfoI RGCGCY 1 cut(s) 1748
BfrI CTTAAG 1 cut(s) 1537
BglI GCCNNNNNGGC 1 cut(s) 422
BisI GCNGC 6 cut(s) 532, 645, 724, 1360, 1828, 2189
BlsI GCNGC 6 cut(s) 533, 646, 725, 1361, 1829, 2190
Bme1390I CCNGG 2 cut(s) 1946, 2147
Bme18I GGWCC 1 cut(s) 1759
BmgT120I GGNCC 3 cut(s) 414, 415, 1759
BmiI GGNNCC 4 cut(s) 7, 416, 1260, 1760
BmrFI CCNGG 2 cut(s) 1946, 2147
BmrI ACTGGG 1 cut(s) 53
BmsI GCATC 4 cut(s) 325, 421, 1407, 1417
BmuI ACTGGG 1 cut(s) 53
BpiI GAAGAC 1 cut(s) 647
Bpu10I CCTNAGC 1 cut(s) 1641
BpuEI CTTGAG 3 cut(s) 420, 1370, 2176
Bsa29I ATCGAT 1 cut(s) 792
BsaAI YACGTR 1 cut(s) 1590
BsaHI GRCGYC 1 cut(s) 471
BsaI GGTCTC 1 cut(s) 937
BsaJI CCNNGG 1 cut(s) 2145
BsaXI ACNNNNNCTCC 2 cut(s) 615, 645
Bsc4I CCNNNNNNNGG 1 cut(s) 115
Bse1I ACTGG 2 cut(s) 59, 910
Bse3DI GCAATG 2 cut(s) 399, 1560
BseBI CCWGG 2 cut(s) 1946, 2147
BseCI ATCGAT 1 cut(s) 792
BseDI CCNNGG 1 cut(s) 2145
BseGI GGATG 3 cut(s) 436, 1345, 1375
BseLI CCNNNNNNNGG 1 cut(s) 115
BseMI GCAATG 2 cut(s) 399, 1560
BseMII CTCAG 3 cut(s) 86, 816, 2052
BseNI ACTGG 2 cut(s) 59, 910
BseSI GKGCMC 2 cut(s) 8, 418
BseXI GCAGC 5 cut(s) 518, 631, 735, 1346, 2175
BsgI GTGCAG 1 cut(s) 158
BshFI GGCC 2 cut(s) 16, 416
BshNI GGYRCC 1 cut(s) 5
BshVI ATCGAT 1 cut(s) 792
BslFI GGGAC 2 cut(s) 1743, 1772
BslI CCNNNNNNNGG 1 cut(s) 115
BsmAI GTCTC 2 cut(s) 478, 937
BsmBI CGTCTC 1 cut(s) 478
BsmFI GGGAC 2 cut(s) 1743, 1772
BsmI GAATGC 2 cut(s) 1701, 2165
BsnI GGCC 2 cut(s) 16, 416
Bso31I GGTCTC 1 cut(s) 937
Bsp120I GGGCCC 1 cut(s) 414
Bsp1286I GDGCHC 2 cut(s) 8, 418
Bsp1407I TGTACA 1 cut(s) 839
Bsp143I GATC 4 cut(s) 77, 1213, 1272, 2108
BspACI CCGC 5 cut(s) 109, 726, 1336, 1828, 2170
BspANI GGCC 2 cut(s) 16, 416
BspCNI CTCAG 3 cut(s) 85, 817, 2053
BspDI ATCGAT 1 cut(s) 792
BspLI GGNNCC 4 cut(s) 7, 416, 1260, 1760
BspMAI CTGCAG 2 cut(s) 1252, 2193
BspPI GGATC 2 cut(s) 72, 2103
BspT107I GGYRCC 1 cut(s) 5
BspTI CTTAAG 1 cut(s) 1537
BspTNI GGTCTC 1 cut(s) 937
BsrBI CCGCTC 1 cut(s) 1830
BsrDI GCAATG 2 cut(s) 399, 1560
BsrGI TGTACA 1 cut(s) 839
BsrI ACTGG 2 cut(s) 59, 910
BssECI CCNNGG 1 cut(s) 2145
BssMI GATC 4 cut(s) 77, 1213, 1272, 2108
BssNI GRCGYC 1 cut(s) 471
BssSI CACGAG 1 cut(s) 134
Bst2BI CACGAG 1 cut(s) 134
Bst2UI CCWGG 2 cut(s) 1946, 2147
Bst4CI ACNGT 2 cut(s) 381, 1810
Bst6I CTCTTC 2 cut(s) 458, 1445
BstACI GRCGYC 1 cut(s) 471
BstAFI CTTAAG 1 cut(s) 1537
BstAUI TGTACA 1 cut(s) 839
BstBAI YACGTR 1 cut(s) 1590
BstC8I GCNNGC 3 cut(s) 570, 1252, 1422
BstDEI CTNAG 8 cut(s) 72, 449, 611, 825, 933, 1641, 1861, 2061
BstF5I GGATG 3 cut(s) 436, 1345, 1375
BstH2I RGCGCY 1 cut(s) 1748
BstHHI GCGC 1 cut(s) 1747
BstKTI GATC 4 cut(s) 80, 1216, 1275, 2111
BstMAI GTCTC 2 cut(s) 478, 937
BstMBI GATC 4 cut(s) 77, 1213, 1272, 2108
BstMWI GCNNNNNNNGC 8 cut(s) 22, 145, 422, 431, 522, 531, 605, 1827
BstNI CCWGG 2 cut(s) 1946, 2147
BstNSI RCATGY 1 cut(s) 2141
BstSCI CCNGG 2 cut(s) 1944, 2145
BstSFI CTRYAG 6 cut(s) 511, 1248, 1720, 1822, 2035, 2189
BstSLI GKGCMC 2 cut(s) 8, 418
BstV1I GCAGC 5 cut(s) 518, 631, 735, 1346, 2175
BstV2I GAAGAC 1 cut(s) 647
BstX2I RGATCY 2 cut(s) 77, 2108
BstYI RGATCY 2 cut(s) 77, 2108
Bsu15I ATCGAT 1 cut(s) 792
BsuRI GGCC 2 cut(s) 16, 416
BsuTUI ATCGAT 1 cut(s) 792
BtsCI GGATG 3 cut(s) 436, 1345, 1375
BtsI GCAGTG 1 cut(s) 1073
BtsIMutI CAGTG 3 cut(s) 66, 1073, 1971
Cac8I GCNNGC 3 cut(s) 570, 1252, 1422
CaiI CAGNNNCTG 1 cut(s) 1115
CfoI GCGC 1 cut(s) 1747
Cfr13I GGNCC 3 cut(s) 414, 415, 1759
ClaI ATCGAT 1 cut(s) 792
CseI GACGC 1 cut(s) 460
Csp6I GTAC 4 cut(s) 150, 840, 1591, 2204
CviQI GTAC 4 cut(s) 150, 840, 1591, 2204
DdeI CTNAG 8 cut(s) 72, 449, 611, 825, 933, 1641, 1861, 2061
DpnI GATC 4 cut(s) 79, 1215, 1274, 2110
DpnII GATC 4 cut(s) 77, 1213, 1272, 2108
DraI TTTAAA 1 cut(s) 1395
DraIII CACNNNGTG 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 458, 1445
EarI CTCTTC 2 cut(s) 458, 1445
Eco24I GRGCYC 1 cut(s) 418
Eco31I GGTCTC 1 cut(s) 937
Eco47I GGWCC 1 cut(s) 1759
Eco57I CTGAAG 2 cut(s) 1137, 1156
EcoO109I RGGNCCY 1 cut(s) 414
EcoRI GAATTC 1 cut(s) 2216
EcoRII CCWGG 2 cut(s) 1944, 2145
EcoT38I GRGCYC 1 cut(s) 418
Esp3I CGTCTC 1 cut(s) 478
FaqI GGGAC 2 cut(s) 1743, 1772
FauI CCCGC 2 cut(s) 719, 1329
FauNDI CATATG 2 cut(s) 1696, 1852
FbaI TGATCA 1 cut(s) 1213
FblI GTMKAC 2 cut(s) 2038, 2047
Fnu4HI GCNGC 6 cut(s) 532, 645, 724, 1360, 1828, 2189
FokI GGATG 3 cut(s) 443, 1352, 1382
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 6 cut(s) 532, 645, 724, 1360, 1828, 2189
FspBI CTAG 5 cut(s) 528, 573, 1668, 1793, 2221
GlaI GCGC 1 cut(s) 1746
GluI GCNGC 6 cut(s) 532, 645, 724, 1360, 1828, 2189
HaeII RGCGCY 1 cut(s) 1748
HaeIII GGCC 2 cut(s) 16, 416
HgaI GACGC 1 cut(s) 460
HhaI GCGC 1 cut(s) 1747
Hin1I GRCGYC 1 cut(s) 471
Hin6I GCGC 1 cut(s) 1745
HinP1I GCGC 1 cut(s) 1745
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HindIII AAGCTT 3 cut(s) 206, 438, 903
HinfI GANTC 5 cut(s) 188, 397, 494, 1611, 1716
HphI GGTGA 2 cut(s) 1637, 1847
Hpy166II GTNNAC 3 cut(s) 337, 2039, 2048
Hpy188I TCNGA 9 cut(s) 75, 187, 327, 360, 430, 817, 1111, 1321, 1917
Hpy8I GTNNAC 3 cut(s) 337, 2039, 2048
HpyCH4III ACNGT 2 cut(s) 381, 1810
HpyCH4IV ACGT 3 cut(s) 560, 1589, 2120
HpyF10VI GCNNNNNNNGC 8 cut(s) 22, 145, 422, 431, 522, 531, 605, 1827
HpyF3I CTNAG 8 cut(s) 72, 449, 611, 825, 933, 1641, 1861, 2061
HpySE526I ACGT 3 cut(s) 560, 1589, 2120
Hsp92I GRCGYC 1 cut(s) 471
HspAI GCGC 1 cut(s) 1745
Ksp22I TGATCA 1 cut(s) 1213
Kzo9I GATC 4 cut(s) 77, 1213, 1272, 2108
LmnI GCTCC 1 cut(s) 1645
Lsp1109I GCAGC 5 cut(s) 518, 631, 735, 1346, 2175
LweI GCATC 4 cut(s) 325, 421, 1407, 1417
MaeI CTAG 5 cut(s) 528, 573, 1668, 1793, 2221
MaeII ACGT 3 cut(s) 560, 1589, 2120
MalI GATC 4 cut(s) 79, 1215, 1274, 2110
MbiI CCGCTC 1 cut(s) 1830
MboI GATC 4 cut(s) 77, 1213, 1272, 2108
MboII GAAGA 9 cut(s) 84, 475, 647, 911, 1426, 1432, 1683, 1784, 1949
MflI RGATCY 2 cut(s) 77, 2108
MhlI GDGCHC 2 cut(s) 8, 418
MlyI GAGTC 1 cut(s) 503
MroXI GAANNNNTTC 1 cut(s) 424
MslI CAYNNNNRTG 1 cut(s) 1793
MspA1I CMGCKG 1 cut(s) 726
MspCI CTTAAG 1 cut(s) 1537
MspR9I CCNGG 2 cut(s) 1946, 2147
Mva1269I GAATGC 2 cut(s) 1701, 2165
MvaI CCWGG 2 cut(s) 1946, 2147
MwoI GCNNNNNNNGC 8 cut(s) 22, 145, 422, 431, 522, 531, 605, 1827
NdeI CATATG 2 cut(s) 1696, 1852
NdeII GATC 4 cut(s) 77, 1213, 1272, 2108
NlaIV GGNNCC 4 cut(s) 7, 416, 1260, 1760
NmuCI GTSAC 8 cut(s) 375, 850, 1585, 1625, 1655, 1795, 1835, 2140
NspI RCATGY 1 cut(s) 2141
OliI CACNNNNGTG 1 cut(s) 1793
PciI ACATGT 1 cut(s) 2137
PctI GAATGC 2 cut(s) 1701, 2165
PdmI GAANNNNTTC 1 cut(s) 424
PfeI GAWTC 4 cut(s) 188, 397, 1611, 1716
PflFI GACNNNGTC 1 cut(s) 1404
PkrI GCNGC 6 cut(s) 533, 646, 725, 1361, 1829, 2190
PleI GAGTC 1 cut(s) 502
PpsI GAGTC 1 cut(s) 502
Ppu21I YACGTR 1 cut(s) 1590
PscI ACATGT 1 cut(s) 2137
PshBI ATTAAT 2 cut(s) 1920, 2126
Psp6I CCWGG 2 cut(s) 1944, 2145
PspGI CCWGG 2 cut(s) 1944, 2145
PspN4I GGNNCC 4 cut(s) 7, 416, 1260, 1760
PspOMI GGGCCC 1 cut(s) 414
PspPI GGNCC 3 cut(s) 414, 415, 1759
PstI CTGCAG 2 cut(s) 1252, 2193
PstNI CAGNNNCTG 1 cut(s) 1115
PsuI RGATCY 2 cut(s) 77, 2108
PsyI GACNNNGTC 1 cut(s) 1404
RsaI GTAC 4 cut(s) 151, 841, 1592, 2205
RsaNI GTAC 4 cut(s) 150, 840, 1591, 2204
RseI CAYNNNNRTG 1 cut(s) 1793
SatI GCNGC 6 cut(s) 532, 645, 724, 1360, 1828, 2189
Sau3AI GATC 4 cut(s) 77, 1213, 1272, 2108
Sau96I GGNCC 3 cut(s) 414, 415, 1759
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 2 cut(s) 1946, 2147
SduI GDGCHC 2 cut(s) 8, 418
SfaNI GCATC 4 cut(s) 325, 421, 1407, 1417
SfcI CTRYAG 6 cut(s) 511, 1248, 1720, 1822, 2035, 2189
SinI GGWCC 1 cut(s) 1759
SmiMI CAYNNNNRTG 1 cut(s) 1793
SmlI CTYRAG 4 cut(s) 435, 1349, 1537, 2155
SmoI CTYRAG 4 cut(s) 435, 1349, 1537, 2155
SsiI CCGC 5 cut(s) 109, 726, 1336, 1828, 2170
SspI AATATT 1 cut(s) 1105
SspMI CTAG 5 cut(s) 528, 573, 1668, 1793, 2221
StyD4I CCNGG 2 cut(s) 1944, 2145
TaaI ACNGT 2 cut(s) 381, 1810
TaiI ACGT 3 cut(s) 563, 1592, 2123
TaqI TCGA 3 cut(s) 635, 792, 1548
TatI WGTACW 1 cut(s) 839
TauI GCSGC 1 cut(s) 1830
TfiI GAWTC 4 cut(s) 188, 397, 1611, 1716
TscAI CASTG 3 cut(s) 66, 1080, 1971
TseFI GTSAC 8 cut(s) 375, 850, 1585, 1625, 1655, 1795, 1835, 2140
TseI GCWGC 5 cut(s) 531, 644, 723, 1359, 2188
Tsp45I GTSAC 8 cut(s) 375, 850, 1585, 1625, 1655, 1795, 1835, 2140
TspDTI ATGAA 7 cut(s) 799, 1256, 1356, 1490, 1683, 2070, 2196
TspGWI ACGGA 1 cut(s) 1308
TspRI CASTG 3 cut(s) 66, 1080, 1971
Tth111I GACNNNGTC 1 cut(s) 1404
Vha464I CTTAAG 1 cut(s) 1537
VpaK11BI GGWCC 1 cut(s) 1759
VspI ATTAAT 2 cut(s) 1920, 2126
XapI RAATTY 4 cut(s) 329, 361, 1122, 2216
XbaI TCTAGA 1 cut(s) 1667
XceI RCATGY 1 cut(s) 2141
XmiI GTMKAC 2 cut(s) 2038, 2047
XmnI GAANNNNTTC 1 cut(s) 424
XspI CTAG 5 cut(s) 528, 573, 1668, 1793, 2221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.