pycom14g04650

Actin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0000536
Physical Location & Seq
Forward (+)
4349600 .. 4351041
1442 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g04650.1

Sequence Viewer

Length: 1083 bp
ATGCAGGCTGGATTTGCTGGAGATGATGCTCCAAGGGCTGTATTTCCGAGCATTGTAGGTCGTCCTCGTCACACTGGTGTGATGGTTGGTATGGGTCAGAAGGATGCCTATGTAGGGGATGAGGCTCAATCGAAGAGAGGTATTTTGACCTTGAAATATCCAATTGAGCACGGTATTGTAAGCAATTGGGATGACATGGAGAAGATCGGGCATCATACATTCTACAATGAGCTCCGTGTGGCTCCGGAAGAGCATCCAGTTCTCCTGACAAAAGCACCCCTTAACCCCAAGGCCAATCGTGAAAAAATGACTCAAATTATGTTTGAGACATTCAACACCCCGGCTATGTACGTTGCAATCCAGGCTGTCCTTTCCCTATATGCTAGTGGGCGTACAACTGGTATTGTTCTCGACTCAGGGGATGGTGTGAGCCACACAGTTCCAATTTATGAAGGGTATGCCCTCCCACATGCCATCCTTCGTTTGGACCTGGCAGGTCGTGATCTTACAGATGCTCTCATGAAAATCTTGACTGAACGTGGTTACTCTGTCACCACAACTGCTGAGCGGGAAATTGTGAGGGACATGAAGGAGAAGTTAGCCTATATTGCTCTTGACTATGAACAAGAGCTAGAGACATCAAAGACCAGCTCGTCTGTGGAGAAGAGTTACGAGCTACCTGATGGACAGGTAATCACTATCGGAGCTGAGAGATTCCGGTGCCCTGAAGTCCTCTTCCAGCCATCCATGATTGGAATGGAAGCTGCCGGTATCCATGAAACCACATACAACTCTATCATGAAGTGTGATGTTGATATTAGGAAGGATCTGTATGGAAATATTGTCCTCAGTGGAGGCTCCACTATGTTCCCTGGAATTGCTGACAGGATGAGCAAAGAGATTACAGCCTTGGCTCCAAGTAGCATGAAGATTAAGGTCGTAGCACCACCTGAGAGGAAATACAGTGTCTGGATTGGAGGCTCCATTTTAGCATCCCTCAGCACCTTCCAACAGGTAATTTGTCGCGCGCGTATGTGTGCGTCTTTTCAATTGAAAACAGCTAAAACACCTCTTCTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

361

Amino Acids

39.66

Weight (kDa)

6.27

Isoelectric Point (pI)

31.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 2 - 339 1.4e-123 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000536)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37620 AT2G37620 AT2G37620 AT2G37620 AT3G46520 AT3G46520 AT3G53750 AT3G53750 AT5G59370 AT5G59370
fragaria_vesca FvH4_3g35960 FvH4_6g13940 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_7g22410 FvH4_7g22410 FvH4_7g22410
malus_domestica MD03G1095300.v1.1 MD04G1127400.v1.1 MD11G1110300.v1.1 MD12G1056900.v1.1 MD12G1140800.v1.1 MD14G1056700.v1.1
prunus_persica Prupe.6G078800_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.7G077300_v2.0.a1 Prupe.7G077300_v2.0.a1
pyrus_communis pycom01g02640 pycom03g07780 pycom11g09110 pycom126g00040 pycom14g04650 pycom15g30330
rosa_chinensis RchiOBHm_Chr1g0367041 RchiOBHm_Chr3g0466761 RchiOBHm_Chr3g0479651 RchiOBHm_Chr5g0064311
rosa_laevigata RLG00000023550 RLG00000024555 RLG00000027304 RLG00000035715
rosa_multiflora Rmu_co8518979.1_g000001 Rmu_sc0002269.1_g000004 Rmu_sc0003467.1_g000022 Rmu_sc0003755.1_g000002 Rmu_sc0013440.1_g000001 Rmu_sc0013700.1_g000002 Rmu_sc0026659.1_g000001 Rmu_sc0037142.1_g000001
rosa_roxburghii Rroxscaffold_1G00016700 Rroxscaffold_4G00289680 Rroxscaffold_6G00402090
rosa_rugosa Rorug01G0334100 Rorug03G0083700 Rorug03G0083800 Rorug03G0177100 Rorug03G0177200 Rorug05G0364000 Rorug05G0364100
rosa_samantha Rh1AG340900 Rh1BG301400 Rh1CG317600 Rh1DG333800 Rh2AG276500 Rh3AG130800 Rh3AG227900 Rh3BG151800 Rh3BG261100 Rh3CG151700 Rh3DG152500 Rh3DG254400 Rh5AG421900 Rh5BG437600 Rh5CG460300 Rh5DG450700
rosa_wichuraiana Rw0G023870 Rw1G030280 Rw3G012360 Rw3G020390 Rw5G039710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 652
Acc36I ACCTGC 1 cut(s) 485
AccB1I GGYRCC 1 cut(s) 720
AccBSI CCGCTC 1 cut(s) 568
AccII CGCG 3 cut(s) 1026, 1028, 1030
AccIII TCCGGA 1 cut(s) 244
AciI CCGC 1 cut(s) 568
AclWI GGATC 1 cut(s) 834
AcuI CTGAAG 1 cut(s) 747
AfaI GTAC 2 cut(s) 350, 394
AfiI CCNNNNNNNGG 2 cut(s) 114, 484
AgsI TTSAA 4 cut(s) 154, 334, 1049, 1054
AjnI CCWGG 3 cut(s) 360, 489, 871
AleI CACNNNNGTG 2 cut(s) 75, 77
AluBI AGCT 7 cut(s) 232, 631, 651, 676, 707, 764, 1061
AluI AGCT 7 cut(s) 232, 631, 651, 676, 707, 764, 1061
Alw21I GWGCWC 2 cut(s) 171, 234
Alw26I GTCTC 2 cut(s) 320, 629
AlwI GGATC 1 cut(s) 834
AlwNI CAGNNNCTG 1 cut(s) 969
Aor13HI TCCGGA 1 cut(s) 244
AoxI GGCC 1 cut(s) 291
ApeKI GCWGC 1 cut(s) 764
AspLEI GCGC 2 cut(s) 1028, 1030
AspS9I GGNCC 1 cut(s) 487
AsuC2I CCSGG 1 cut(s) 341
AsuHPI GGTGA 1 cut(s) 544
AvaII GGWCC 1 cut(s) 487
BaeGI GKGCMC 1 cut(s) 725
BanI GGYRCC 1 cut(s) 720
BanII GRGCYC 1 cut(s) 234
Bbv12I GWGCWC 2 cut(s) 171, 234
BbvCI CCTCAGC 1 cut(s) 998
BbvI GCAGC 1 cut(s) 751
BccI CCATC 5 cut(s) 76, 416, 482, 677, 751
BciT130I CCWGG 3 cut(s) 362, 491, 873
BciVI GTATCC 1 cut(s) 782
BcnI CCSGG 1 cut(s) 341
BcoDI GTCTC 2 cut(s) 320, 629
BfaI CTAG 2 cut(s) 384, 632
BfuAI ACCTGC 1 cut(s) 485
BfuI GTATCC 1 cut(s) 782
BisI GCNGC 1 cut(s) 765
BlpI GCTNAGC 1 cut(s) 564
BlsI GCNGC 1 cut(s) 766
Bme1390I CCNGG 4 cut(s) 341, 362, 491, 873
Bme18I GGWCC 1 cut(s) 487
BmgT120I GGNCC 1 cut(s) 487
BmiI GGNNCC 5 cut(s) 243, 722, 859, 915, 982
BmrFI CCNGG 4 cut(s) 341, 362, 491, 873
BmsI GCATC 6 cut(s) 16, 94, 220, 262, 502, 1001
BpmI CTGGAG 1 cut(s) 39
Bpu10I CCTNAGC 1 cut(s) 998
Bpu1102I GCTNAGC 1 cut(s) 564
BpuMI CCSGG 1 cut(s) 341
BsaJI CCNNGG 5 cut(s) 32, 288, 339, 871, 909
BsaWI WCCGGW 2 cut(s) 244, 717
Bsc4I CCNNNNNNNGG 2 cut(s) 114, 484
Bse118I RCCGGY 1 cut(s) 767
Bse1I ACTGG 3 cut(s) 79, 257, 403
BseAI TCCGGA 1 cut(s) 244
BseBI CCWGG 3 cut(s) 362, 491, 873
BseDI CCNNGG 5 cut(s) 32, 288, 339, 871, 909
BseGI GGATG 9 cut(s) 109, 124, 196, 253, 427, 474, 743, 894, 992
BseLI CCNNNNNNNGG 2 cut(s) 114, 484
BseMII CTCAG 6 cut(s) 429, 555, 699, 862, 942, 1012
BseNI ACTGG 3 cut(s) 79, 257, 403
BsePI GCGCGC 1 cut(s) 1026
BseSI GKGCMC 1 cut(s) 725
BseXI GCAGC 1 cut(s) 751
Bsh1236I CGCG 3 cut(s) 1026, 1028, 1030
BshFI GGCC 1 cut(s) 293
BshNI GGYRCC 1 cut(s) 720
BsiHKAI GWGCWC 2 cut(s) 171, 234
BsiSI CCGG 4 cut(s) 245, 341, 718, 768
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 2 cut(s) 114, 484
BsmAI GTCTC 2 cut(s) 320, 629
BsmFI GGGAC 1 cut(s) 596
BsnI GGCC 1 cut(s) 293
Bsp1286I GDGCHC 3 cut(s) 171, 234, 725
Bsp13I TCCGGA 1 cut(s) 244
Bsp143I GATC 3 cut(s) 204, 502, 826
Bsp1720I GCTNAGC 1 cut(s) 564
BspACI CCGC 1 cut(s) 568
BspANI GGCC 1 cut(s) 293
BspCNI CTCAG 6 cut(s) 428, 556, 700, 861, 943, 1011
BspEI TCCGGA 1 cut(s) 244
BspFNI CGCG 3 cut(s) 1026, 1028, 1030
BspHI TCATGA 2 cut(s) 519, 798
BspLI GGNNCC 5 cut(s) 243, 722, 859, 915, 982
BspMI ACCTGC 1 cut(s) 485
BspPI GGATC 1 cut(s) 834
BspQI GCTCTTC 1 cut(s) 243
BspT107I GGYRCC 1 cut(s) 720
BsrBI CCGCTC 1 cut(s) 568
BsrFI RCCGGY 1 cut(s) 767
BsrI ACTGG 3 cut(s) 79, 257, 403
BssAI RCCGGY 1 cut(s) 767
BssECI CCNNGG 5 cut(s) 32, 288, 339, 871, 909
BssHII GCGCGC 1 cut(s) 1026
BssMI GATC 3 cut(s) 204, 502, 826
BssT1I CCWWGG 3 cut(s) 32, 288, 909
Bst2UI CCWGG 3 cut(s) 362, 491, 873
Bst4CI ACNGT 3 cut(s) 173, 439, 965
Bst6I CTCTTC 5 cut(s) 128, 243, 659, 740, 1077
BstC8I GCNNGC 2 cut(s) 6, 1028
BstDEI CTNAG 6 cut(s) 415, 564, 708, 848, 951, 998
BstF5I GGATG 9 cut(s) 109, 124, 196, 253, 427, 474, 743, 894, 992
BstFNI CGCG 3 cut(s) 1026, 1028, 1030
BstHHI GCGC 2 cut(s) 1028, 1030
BstKTI GATC 3 cut(s) 207, 505, 829
BstMAI GTCTC 2 cut(s) 320, 629
BstMBI GATC 3 cut(s) 204, 502, 826
BstMWI GCNNNNNNNGC 4 cut(s) 14, 35, 362, 608
BstNI CCWGG 3 cut(s) 362, 491, 873
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 4 cut(s) 339, 360, 489, 871
BstSLI GKGCMC 1 cut(s) 725
BstUI CGCG 3 cut(s) 1026, 1028, 1030
BstV1I GCAGC 1 cut(s) 751
BstX2I RGATCY 1 cut(s) 826
BstYI RGATCY 1 cut(s) 826
BsuI GTATCC 1 cut(s) 782
BsuRI GGCC 1 cut(s) 293
BtsCI GGATG 9 cut(s) 109, 124, 196, 253, 427, 474, 743, 894, 992
BtsIMutI CAGTG 3 cut(s) 72, 856, 970
BveI ACCTGC 1 cut(s) 485
Cac8I GCNNGC 2 cut(s) 6, 1028
CaiI CAGNNNCTG 1 cut(s) 969
CciI TCATGA 2 cut(s) 519, 798
CfoI GCGC 2 cut(s) 1028, 1030
Cfr10I RCCGGY 1 cut(s) 767
Cfr13I GGNCC 1 cut(s) 487
CseI GACGC 1 cut(s) 1029
Csp6I GTAC 2 cut(s) 349, 393
CviAII CATG 8 cut(s) 196, 470, 520, 586, 748, 776, 799, 925
CviQI GTAC 2 cut(s) 349, 393
DdeI CTNAG 6 cut(s) 415, 564, 708, 848, 951, 998
DpnI GATC 3 cut(s) 206, 504, 828
DpnII GATC 3 cut(s) 204, 502, 826
DrdI GACNNNNNNGTC 1 cut(s) 652
DseDI GACNNNNNNGTC 1 cut(s) 652
Eam1104I CTCTTC 5 cut(s) 128, 243, 659, 740, 1077
EarI CTCTTC 5 cut(s) 128, 243, 659, 740, 1077
Ecl136II GAGCTC 1 cut(s) 232
Eco130I CCWWGG 3 cut(s) 32, 288, 909
Eco24I GRGCYC 1 cut(s) 234
Eco47I GGWCC 1 cut(s) 487
Eco53kI GAGCTC 1 cut(s) 232
Eco57I CTGAAG 1 cut(s) 747
EcoICRI GAGCTC 1 cut(s) 232
EcoRII CCWGG 3 cut(s) 360, 489, 871
EcoT14I CCWWGG 3 cut(s) 32, 288, 909
EcoT38I GRGCYC 1 cut(s) 234
ErhI CCWWGG 3 cut(s) 32, 288, 909
FaeI CATG 8 cut(s) 199, 473, 523, 589, 751, 779, 802, 928
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FaqI GGGAC 1 cut(s) 596
FatI CATG 8 cut(s) 195, 469, 519, 585, 747, 775, 798, 924
FauI CCCGC 1 cut(s) 561
Fnu4HI GCNGC 1 cut(s) 765
FokI GGATG 9 cut(s) 116, 131, 203, 240, 434, 461, 730, 901, 979
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 1 cut(s) 765
FspBI CTAG 2 cut(s) 384, 632
GlaI GCGC 2 cut(s) 1027, 1029
GluI GCNGC 1 cut(s) 765
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 1 cut(s) 293
HapII CCGG 4 cut(s) 245, 341, 718, 768
HgaI GACGC 1 cut(s) 1029
HhaI GCGC 2 cut(s) 1028, 1030
Hin1II CATG 8 cut(s) 199, 473, 523, 589, 751, 779, 802, 928
Hin6I GCGC 2 cut(s) 1026, 1028
HinP1I GCGC 2 cut(s) 1026, 1028
HinfI GANTC 3 cut(s) 310, 413, 714
HpaII CCGG 4 cut(s) 245, 341, 718, 768
HphI GGTGA 1 cut(s) 544
Hpy188I TCNGA 3 cut(s) 48, 99, 704
HpyAV CCTTC 6 cut(s) 94, 446, 488, 583, 817, 1015
HpyCH4III ACNGT 3 cut(s) 173, 439, 965
HpyCH4IV ACGT 2 cut(s) 351, 538
HpyCH4V TGCA 2 cut(s) 4, 356
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 35, 362, 608
HpyF3I CTNAG 6 cut(s) 415, 564, 708, 848, 951, 998
HpySE526I ACGT 2 cut(s) 351, 538
Hsp92II CATG 8 cut(s) 199, 473, 523, 589, 751, 779, 802, 928
HspAI GCGC 2 cut(s) 1026, 1028
Kpn2I TCCGGA 1 cut(s) 244
Kzo9I GATC 3 cut(s) 204, 502, 826
LguI GCTCTTC 1 cut(s) 243
LmnI GCTCC 7 cut(s) 34, 237, 247, 704, 863, 919, 986
Lsp1109I GCAGC 1 cut(s) 751
LweI GCATC 6 cut(s) 16, 94, 220, 262, 502, 1001
MaeI CTAG 2 cut(s) 384, 632
MaeII ACGT 2 cut(s) 351, 538
MaeIII GTNAC 4 cut(s) 68, 542, 550, 668
MalI GATC 3 cut(s) 206, 504, 828
MauBI CGCGCGCG 1 cut(s) 1026
MbiI CCGCTC 1 cut(s) 568
MboI GATC 3 cut(s) 204, 502, 826
MboII GAAGA 7 cut(s) 145, 214, 260, 676, 727, 940, 1064
MfeI CAATTG 3 cut(s) 162, 184, 1049
MflI RGATCY 1 cut(s) 826
MhlI GDGCHC 3 cut(s) 171, 234, 725
MluCI AATT 8 cut(s) 162, 184, 315, 444, 573, 876, 1017, 1049
MlyI GAGTC 2 cut(s) 304, 407
MmeI TCCRAC 1 cut(s) 1033
MroI TCCGGA 1 cut(s) 244
MseI TTAA 3 cut(s) 282, 933, 1081
MslI CAYNNNNRTG 2 cut(s) 75, 77
MspI CCGG 4 cut(s) 245, 341, 718, 768
MspR9I CCNGG 4 cut(s) 341, 362, 491, 873
MunI CAATTG 3 cut(s) 162, 184, 1049
MvaI CCWGG 3 cut(s) 362, 491, 873
MvnI CGCG 3 cut(s) 1026, 1028, 1030
MwoI GCNNNNNNNGC 4 cut(s) 14, 35, 362, 608
NciI CCSGG 1 cut(s) 341
NdeII GATC 3 cut(s) 204, 502, 826
NlaIII CATG 8 cut(s) 199, 473, 523, 589, 751, 779, 802, 928
NlaIV GGNNCC 5 cut(s) 243, 722, 859, 915, 982
NmuCI GTSAC 2 cut(s) 68, 550
NspI RCATGY 1 cut(s) 473
OliI CACNNNNGTG 2 cut(s) 75, 77
PagI TCATGA 2 cut(s) 519, 798
PauI GCGCGC 1 cut(s) 1026
PciSI GCTCTTC 1 cut(s) 243
PfeI GAWTC 1 cut(s) 714
PkrI GCNGC 1 cut(s) 766
PleI GAGTC 2 cut(s) 304, 407
PpsI GAGTC 2 cut(s) 304, 407
Psp124BI GAGCTC 1 cut(s) 234
Psp6I CCWGG 3 cut(s) 360, 489, 871
PspGI CCWGG 3 cut(s) 360, 489, 871
PspN4I GGNNCC 5 cut(s) 243, 722, 859, 915, 982
PspPI GGNCC 1 cut(s) 487
PstNI CAGNNNCTG 1 cut(s) 969
PsuI RGATCY 1 cut(s) 826
PteI GCGCGC 1 cut(s) 1026
RsaI GTAC 2 cut(s) 350, 394
RsaNI GTAC 2 cut(s) 349, 393
RseI CAYNNNNRTG 2 cut(s) 75, 77
SacI GAGCTC 1 cut(s) 234
SapI GCTCTTC 1 cut(s) 243
SaqAI TTAA 3 cut(s) 282, 933, 1081
SatI GCNGC 1 cut(s) 765
Sau3AI GATC 3 cut(s) 204, 502, 826
Sau96I GGNCC 1 cut(s) 487
SchI GAGTC 2 cut(s) 304, 407
ScrFI CCNGG 4 cut(s) 341, 362, 491, 873
SduI GDGCHC 3 cut(s) 171, 234, 725
SfaNI GCATC 6 cut(s) 16, 94, 220, 262, 502, 1001
SinI GGWCC 1 cut(s) 487
SmiMI CAYNNNNRTG 2 cut(s) 75, 77
Sse9I AATT 8 cut(s) 162, 184, 315, 444, 573, 876, 1017, 1049
SsiI CCGC 1 cut(s) 568
SspI AATATT 1 cut(s) 841
SspMI CTAG 2 cut(s) 384, 632
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 4 cut(s) 339, 360, 489, 871
StyI CCWWGG 3 cut(s) 32, 288, 909
TaaI ACNGT 3 cut(s) 173, 439, 965
TaiI ACGT 2 cut(s) 354, 541
TaqI TCGA 2 cut(s) 131, 411
TasI AATT 8 cut(s) 162, 184, 315, 444, 573, 876, 1017, 1049
TfiI GAWTC 1 cut(s) 714
Tru1I TTAA 3 cut(s) 282, 933, 1081
Tru9I TTAA 3 cut(s) 282, 933, 1081
TscAI CASTG 3 cut(s) 79, 856, 970
TseFI GTSAC 2 cut(s) 68, 550
TseI GCWGC 1 cut(s) 764
Tsp45I GTSAC 2 cut(s) 68, 550
TspDTI ATGAA 7 cut(s) 465, 536, 602, 636, 792, 815, 941
TspGWI ACGGA 1 cut(s) 224
TspRI CASTG 3 cut(s) 79, 856, 970
VpaK11BI GGWCC 1 cut(s) 487
XceI RCATGY 1 cut(s) 473
XcmI CCANNNNNNNNNTGG 3 cut(s) 481, 655, 754
XspI CTAG 2 cut(s) 384, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.