MD14G1056700.v1.1

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000536
Physical Location & Seq
Forward (+)
5854581 .. 5857336
2756 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1056700.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGGTCAAGGCTGGATTTGCTGGAGATGATGCTCCAAGGGCTGTATTTCCCAGCATTGTAGGTCGTCCTCGTCACACTGGTGTGATGGTTGGTATGGGTCAGAAGGATGCCTACGTAGGGGATGAGGCTCAATCGAAGAGAGGTATTTTGACCTTGAAATATCCAATTGAGCACGGTATTGTAAGCAATTGGGATGACATGGAGAAGATCTGGCATCACACATTCTACAATGAGCTCCGTGTGGCGCCGGAAGAGCATCCAGTTCTCCTGACAGAAGCACCCCTTAACCCCAAGGCCAATCGTGAAAAAATGACTCAAATTATGTTTGAGACATTCAACACCCCGGCTATGTACGTTGCAATCCAGGCTGTCCTTTCCCTATATGCTAGTGGACGTACAACTGGTATTGTTCTCGACTCAGGGGATGGTGTGAGCCACACAGTTCCAATTTATGAAGGGTATGCCCTCCCACATGCCATCCTTCGTTTGGACCTGGCAGGACGTGATCTTACAGATGCTCTCATGAAAATCTTGACTGAACGTGGTTACTCTTTCACCACTACTGCTGAGCGGGAAATTGTGAGGGACATGAAGGAGAAGTTAGCCTATATTGCTCTTGACTATGAACAAGAGCTAGAGACATCGAAGACCAGCTCGTCCGTGGAGAAGAGTTACGAGCTACCTGATGGACAGGTAATCACTATCGGAGCTGAGAGATTCCGGTGCCCTGAAGTCCTCTTCCAGCCATCCATGATTGGAATGGAAGCTGCCGGTATCCATGAAACCACATACAACTCTATCATGAAGTGTGATGTTGATATTAGGAAGGATCTGTATGGAAATATTGTCCTCAGTGGAGGCTCCACTATGTTCCCTGGAATTGCTGACAGGATGAGCAAAGAGATTACAGCCTTGGCTCCAAGTAGCATGAAGATTAAGGTCGTAGCACCACCGGAGAGGAAATACAGTGTCTGGATTGGAGGCTCCATTTTAGCATCCCTCAGCACCTTCCAACAGATGTGGATTGCAAAGGCAGAGTACGATGAGTCTGGCCCCTCAATTGTTCACAGGAAATGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

361

Amino Acids

39.96

Weight (kDa)

5.67

Isoelectric Point (pI)

35.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 1 - 360 2.2e-141 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000536)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37620 AT2G37620 AT2G37620 AT2G37620 AT3G46520 AT3G46520 AT3G53750 AT3G53750 AT5G59370 AT5G59370
fragaria_vesca FvH4_3g35960 FvH4_6g13940 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_7g22410 FvH4_7g22410 FvH4_7g22410
malus_domestica MD03G1095300.v1.1 MD04G1127400.v1.1 MD11G1110300.v1.1 MD12G1056900.v1.1 MD12G1140800.v1.1 MD14G1056700.v1.1
prunus_persica Prupe.6G078800_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.7G077300_v2.0.a1 Prupe.7G077300_v2.0.a1
pyrus_communis pycom01g02640 pycom03g07780 pycom11g09110 pycom126g00040 pycom14g04650 pycom15g30330
rosa_chinensis RchiOBHm_Chr1g0367041 RchiOBHm_Chr3g0466761 RchiOBHm_Chr3g0479651 RchiOBHm_Chr5g0064311
rosa_laevigata RLG00000023550 RLG00000024555 RLG00000027304 RLG00000035715
rosa_multiflora Rmu_co8518979.1_g000001 Rmu_sc0002269.1_g000004 Rmu_sc0003467.1_g000022 Rmu_sc0003755.1_g000002 Rmu_sc0013440.1_g000001 Rmu_sc0013700.1_g000002 Rmu_sc0026659.1_g000001 Rmu_sc0037142.1_g000001
rosa_roxburghii Rroxscaffold_1G00016700 Rroxscaffold_4G00289680 Rroxscaffold_6G00402090
rosa_rugosa Rorug01G0334100 Rorug03G0083700 Rorug03G0083800 Rorug03G0177100 Rorug03G0177200 Rorug05G0364000 Rorug05G0364100
rosa_samantha Rh1AG340900 Rh1BG301400 Rh1CG317600 Rh1DG333800 Rh2AG276500 Rh3AG130800 Rh3AG227900 Rh3BG151800 Rh3BG261100 Rh3CG151700 Rh3DG152500 Rh3DG254400 Rh5AG421900 Rh5BG437600 Rh5CG460300 Rh5DG450700
rosa_wichuraiana Rw0G023870 Rw1G030280 Rw3G012360 Rw3G020390 Rw5G039710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 655
AccB1I GGYRCC 2 cut(s) 244, 723
AccBSI CCGCTC 1 cut(s) 571
AciI CCGC 1 cut(s) 571
AclWI GGATC 1 cut(s) 837
AcuI CTGAAG 1 cut(s) 750
AcyI GRCGYC 1 cut(s) 245
AfaI GTAC 3 cut(s) 353, 397, 1040
AfiI CCNNNNNNNGG 2 cut(s) 117, 487
AgsI TTSAA 2 cut(s) 157, 337
AjiI CACGTC 1 cut(s) 503
AjnI CCWGG 3 cut(s) 363, 492, 874
AleI CACNNNNGTG 2 cut(s) 78, 80
AluBI AGCT 6 cut(s) 235, 634, 654, 679, 710, 767
AluI AGCT 6 cut(s) 235, 634, 654, 679, 710, 767
Alw21I GWGCWC 2 cut(s) 174, 237
Alw26I GTCTC 2 cut(s) 323, 632
AlwI GGATC 1 cut(s) 837
AlwNI CAGNNNCTG 1 cut(s) 972
AoxI GGCC 2 cut(s) 294, 1051
ApeKI GCWGC 1 cut(s) 767
Asp700I GAANNNNTTC 1 cut(s) 1076
AspLEI GCGC 1 cut(s) 247
AspS9I GGNCC 2 cut(s) 490, 1052
AsuC2I CCSGG 1 cut(s) 344
AsuHPI GGTGA 1 cut(s) 547
AvaII GGWCC 1 cut(s) 490
BaeGI GKGCMC 1 cut(s) 728
BanI GGYRCC 2 cut(s) 244, 723
BanII GRGCYC 1 cut(s) 237
BarI GAAGNNNNNNTAC 2 cut(s) 95, 127
BbsI GAAGAC 1 cut(s) 653
Bbv12I GWGCWC 2 cut(s) 174, 237
BbvCI CCTCAGC 1 cut(s) 1001
BbvI GCAGC 1 cut(s) 754
BccI CCATC 5 cut(s) 79, 419, 485, 680, 754
BciT130I CCWGG 3 cut(s) 365, 494, 876
BciVI GTATCC 1 cut(s) 785
BcnI CCSGG 1 cut(s) 344
BcoDI GTCTC 2 cut(s) 323, 632
BfaI CTAG 2 cut(s) 387, 635
BfoI RGCGCY 1 cut(s) 248
BfuI GTATCC 1 cut(s) 785
BglII AGATCT 1 cut(s) 207
BisI GCNGC 1 cut(s) 768
BlpI GCTNAGC 1 cut(s) 567
BlsI GCNGC 1 cut(s) 769
Bme1390I CCNGG 4 cut(s) 344, 365, 494, 876
Bme18I GGWCC 1 cut(s) 490
BmgBI CACGTC 1 cut(s) 503
BmgT120I GGNCC 2 cut(s) 490, 1052
BmiI GGNNCC 6 cut(s) 246, 725, 862, 918, 985, 1054
BmrFI CCNGG 4 cut(s) 344, 365, 494, 876
BmsI GCATC 6 cut(s) 19, 97, 223, 265, 505, 1004
BpiI GAAGAC 1 cut(s) 653
BpmI CTGGAG 1 cut(s) 42
Bpu10I CCTNAGC 1 cut(s) 1001
Bpu1102I GCTNAGC 1 cut(s) 567
BpuMI CCSGG 1 cut(s) 344
BsaAI YACGTR 1 cut(s) 115
BsaHI GRCGYC 1 cut(s) 245
BsaJI CCNNGG 6 cut(s) 35, 291, 342, 660, 874, 912
BsaWI WCCGGW 2 cut(s) 720, 952
Bsc4I CCNNNNNNNGG 2 cut(s) 117, 487
Bse118I RCCGGY 1 cut(s) 770
Bse1I ACTGG 3 cut(s) 82, 260, 406
BseBI CCWGG 3 cut(s) 365, 494, 876
BseDI CCNNGG 6 cut(s) 35, 291, 342, 660, 874, 912
BseGI GGATG 9 cut(s) 112, 127, 199, 256, 430, 477, 746, 897, 995
BseLI CCNNNNNNNGG 2 cut(s) 117, 487
BseMII CTCAG 5 cut(s) 432, 558, 702, 865, 1015
BseNI ACTGG 3 cut(s) 82, 260, 406
BseSI GKGCMC 1 cut(s) 728
BseXI GCAGC 1 cut(s) 754
BseYI CCCAGC 1 cut(s) 50
BshFI GGCC 2 cut(s) 296, 1053
BshNI GGYRCC 2 cut(s) 244, 723
BsiHKAI GWGCWC 2 cut(s) 174, 237
BsiSI CCGG 5 cut(s) 248, 344, 721, 771, 953
BslFI GGGAC 1 cut(s) 599
BslI CCNNNNNNNGG 2 cut(s) 117, 487
BsmAI GTCTC 2 cut(s) 323, 632
BsmFI GGGAC 1 cut(s) 599
BsnI GGCC 2 cut(s) 296, 1053
Bsp1286I GDGCHC 3 cut(s) 174, 237, 728
Bsp143I GATC 3 cut(s) 207, 505, 829
Bsp1720I GCTNAGC 1 cut(s) 567
BspACI CCGC 1 cut(s) 571
BspANI GGCC 2 cut(s) 296, 1053
BspCNI CTCAG 5 cut(s) 431, 559, 703, 864, 1014
BspHI TCATGA 2 cut(s) 522, 801
BspLI GGNNCC 6 cut(s) 246, 725, 862, 918, 985, 1054
BspPI GGATC 1 cut(s) 837
BspQI GCTCTTC 1 cut(s) 246
BspT107I GGYRCC 2 cut(s) 244, 723
BsrBI CCGCTC 1 cut(s) 571
BsrFI RCCGGY 1 cut(s) 770
BsrI ACTGG 3 cut(s) 82, 260, 406
BssAI RCCGGY 1 cut(s) 770
BssECI CCNNGG 6 cut(s) 35, 291, 342, 660, 874, 912
BssMI GATC 3 cut(s) 207, 505, 829
BssNI GRCGYC 1 cut(s) 245
BssT1I CCWWGG 3 cut(s) 35, 291, 912
Bst2UI CCWGG 3 cut(s) 365, 494, 876
Bst4CI ACNGT 3 cut(s) 176, 442, 968
Bst6I CTCTTC 4 cut(s) 131, 246, 662, 743
BstACI GRCGYC 1 cut(s) 245
BstBAI YACGTR 1 cut(s) 115
BstDEI CTNAG 5 cut(s) 418, 567, 711, 851, 1001
BstDSI CCRYGG 1 cut(s) 660
BstF5I GGATG 9 cut(s) 112, 127, 199, 256, 430, 477, 746, 897, 995
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 3 cut(s) 210, 508, 832
BstMAI GTCTC 2 cut(s) 323, 632
BstMBI GATC 3 cut(s) 207, 505, 829
BstMWI GCNNNNNNNGC 5 cut(s) 17, 38, 253, 365, 611
BstNI CCWGG 3 cut(s) 365, 494, 876
BstNSI RCATGY 1 cut(s) 476
BstSCI CCNGG 4 cut(s) 342, 363, 492, 874
BstSLI GKGCMC 1 cut(s) 728
BstSNI TACGTA 1 cut(s) 115
BstV1I GCAGC 1 cut(s) 754
BstV2I GAAGAC 1 cut(s) 653
BstX2I RGATCY 2 cut(s) 207, 829
BstYI RGATCY 2 cut(s) 207, 829
BsuI GTATCC 1 cut(s) 785
BsuRI GGCC 2 cut(s) 296, 1053
BtgI CCRYGG 1 cut(s) 660
BtrI CACGTC 1 cut(s) 503
BtsCI GGATG 9 cut(s) 112, 127, 199, 256, 430, 477, 746, 897, 995
BtsIMutI CAGTG 3 cut(s) 75, 859, 973
CaiI CAGNNNCTG 1 cut(s) 972
CciI TCATGA 2 cut(s) 522, 801
CfoI GCGC 1 cut(s) 247
Cfr10I RCCGGY 1 cut(s) 770
Cfr13I GGNCC 2 cut(s) 490, 1052
Csp6I GTAC 3 cut(s) 352, 396, 1039
CspCI CAANNNNNGTGG 2 cut(s) 1001, 1036
CviAII CATG 8 cut(s) 199, 473, 523, 589, 751, 779, 802, 928
CviQI GTAC 3 cut(s) 352, 396, 1039
DdeI CTNAG 5 cut(s) 418, 567, 711, 851, 1001
DinI GGCGCC 1 cut(s) 246
DpnI GATC 3 cut(s) 209, 507, 831
DpnII GATC 3 cut(s) 207, 505, 829
DrdI GACNNNNNNGTC 1 cut(s) 655
DseDI GACNNNNNNGTC 1 cut(s) 655
Eam1104I CTCTTC 4 cut(s) 131, 246, 662, 743
EarI CTCTTC 4 cut(s) 131, 246, 662, 743
Ecl136II GAGCTC 1 cut(s) 235
Eco105I TACGTA 1 cut(s) 115
Eco130I CCWWGG 3 cut(s) 35, 291, 912
Eco24I GRGCYC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 490
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 750
EcoICRI GAGCTC 1 cut(s) 235
EcoRII CCWGG 3 cut(s) 363, 492, 874
EcoT14I CCWWGG 3 cut(s) 35, 291, 912
EcoT38I GRGCYC 1 cut(s) 237
EgeI GGCGCC 1 cut(s) 246
EheI GGCGCC 1 cut(s) 246
ErhI CCWWGG 3 cut(s) 35, 291, 912
FaeI CATG 8 cut(s) 202, 476, 526, 592, 754, 782, 805, 931
FalI AAGNNNNNCTT 2 cut(s) 267, 299
FaqI GGGAC 1 cut(s) 599
FatI CATG 8 cut(s) 198, 472, 522, 588, 750, 778, 801, 927
FauI CCCGC 1 cut(s) 564
Fnu4HI GCNGC 1 cut(s) 768
FokI GGATG 9 cut(s) 119, 134, 206, 243, 437, 464, 733, 904, 982
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 1 cut(s) 768
FspBI CTAG 2 cut(s) 387, 635
GlaI GCGC 1 cut(s) 246
GluI GCNGC 1 cut(s) 768
GsaI CCCAGC 1 cut(s) 54
GsuI CTGGAG 1 cut(s) 42
HaeII RGCGCY 1 cut(s) 248
HaeIII GGCC 2 cut(s) 296, 1053
HapII CCGG 5 cut(s) 248, 344, 721, 771, 953
HhaI GCGC 1 cut(s) 247
Hin1I GRCGYC 1 cut(s) 245
Hin1II CATG 8 cut(s) 202, 476, 526, 592, 754, 782, 805, 931
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HinfI GANTC 4 cut(s) 313, 416, 717, 1046
HpaII CCGG 5 cut(s) 248, 344, 721, 771, 953
HphI GGTGA 1 cut(s) 547
Hpy166II GTNNAC 2 cut(s) 392, 1066
Hpy188I TCNGA 2 cut(s) 102, 707
Hpy188III TCNNGA 8 cut(s) 268, 302, 413, 523, 532, 617, 802, 973
Hpy8I GTNNAC 2 cut(s) 392, 1066
HpyAV CCTTC 6 cut(s) 97, 449, 491, 586, 820, 1018
HpyCH4III ACNGT 3 cut(s) 176, 442, 968
HpyCH4IV ACGT 5 cut(s) 114, 354, 394, 502, 541
HpyCH4V TGCA 2 cut(s) 359, 1028
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 38, 253, 365, 611
HpyF3I CTNAG 5 cut(s) 418, 567, 711, 851, 1001
HpySE526I ACGT 5 cut(s) 114, 354, 394, 502, 541
Hsp92I GRCGYC 1 cut(s) 245
Hsp92II CATG 8 cut(s) 202, 476, 526, 592, 754, 782, 805, 931
HspAI GCGC 1 cut(s) 245
KasI GGCGCC 1 cut(s) 244
Kzo9I GATC 3 cut(s) 207, 505, 829
LguI GCTCTTC 1 cut(s) 246
LmnI GCTCC 6 cut(s) 37, 240, 707, 866, 922, 989
Lsp1109I GCAGC 1 cut(s) 754
LweI GCATC 6 cut(s) 19, 97, 223, 265, 505, 1004
MaeI CTAG 2 cut(s) 387, 635
MaeII ACGT 5 cut(s) 114, 354, 394, 502, 541
MaeIII GTNAC 3 cut(s) 71, 545, 671
MalI GATC 3 cut(s) 209, 507, 831
MbiI CCGCTC 1 cut(s) 571
MboI GATC 3 cut(s) 207, 505, 829
MboII GAAGA 7 cut(s) 148, 217, 263, 658, 679, 730, 943
MfeI CAATTG 3 cut(s) 165, 187, 1059
MflI RGATCY 2 cut(s) 207, 829
MhlI GDGCHC 3 cut(s) 174, 237, 728
MluCI AATT 7 cut(s) 165, 187, 318, 447, 576, 879, 1059
Mly113I GGCGCC 1 cut(s) 245
MlyI GAGTC 3 cut(s) 307, 410, 1055
MmeI TCCRAC 1 cut(s) 1036
MroXI GAANNNNTTC 1 cut(s) 1076
MseI TTAA 2 cut(s) 285, 936
MslI CAYNNNNRTG 2 cut(s) 78, 80
MspI CCGG 5 cut(s) 248, 344, 721, 771, 953
MspR9I CCNGG 4 cut(s) 344, 365, 494, 876
MunI CAATTG 3 cut(s) 165, 187, 1059
MvaI CCWGG 3 cut(s) 365, 494, 876
MwoI GCNNNNNNNGC 5 cut(s) 17, 38, 253, 365, 611
NarI GGCGCC 1 cut(s) 245
NciI CCSGG 1 cut(s) 344
NdeII GATC 3 cut(s) 207, 505, 829
NlaIII CATG 8 cut(s) 202, 476, 526, 592, 754, 782, 805, 931
NlaIV GGNNCC 6 cut(s) 246, 725, 862, 918, 985, 1054
NmuCI GTSAC 1 cut(s) 71
NspI RCATGY 1 cut(s) 476
OliI CACNNNNGTG 2 cut(s) 78, 80
PagI TCATGA 2 cut(s) 522, 801
PciSI GCTCTTC 1 cut(s) 246
PdmI GAANNNNTTC 1 cut(s) 1076
PfeI GAWTC 1 cut(s) 717
PkrI GCNGC 1 cut(s) 769
PleI GAGTC 3 cut(s) 307, 410, 1054
PluTI GGCGCC 1 cut(s) 248
PpsI GAGTC 3 cut(s) 307, 410, 1054
Ppu21I YACGTR 1 cut(s) 115
Psp124BI GAGCTC 1 cut(s) 237
Psp6I CCWGG 3 cut(s) 363, 492, 874
PspFI CCCAGC 1 cut(s) 50
PspGI CCWGG 3 cut(s) 363, 492, 874
PspN4I GGNNCC 6 cut(s) 246, 725, 862, 918, 985, 1054
PspPI GGNCC 2 cut(s) 490, 1052
PstNI CAGNNNCTG 1 cut(s) 972
PsuI RGATCY 2 cut(s) 207, 829
RsaI GTAC 3 cut(s) 353, 397, 1040
RsaNI GTAC 3 cut(s) 352, 396, 1039
RseI CAYNNNNRTG 2 cut(s) 78, 80
SacI GAGCTC 1 cut(s) 237
SapI GCTCTTC 1 cut(s) 246
SaqAI TTAA 2 cut(s) 285, 936
SatI GCNGC 1 cut(s) 768
Sau3AI GATC 3 cut(s) 207, 505, 829
Sau96I GGNCC 2 cut(s) 490, 1052
SchI GAGTC 3 cut(s) 307, 410, 1055
ScrFI CCNGG 4 cut(s) 344, 365, 494, 876
SduI GDGCHC 3 cut(s) 174, 237, 728
SfaNI GCATC 6 cut(s) 19, 97, 223, 265, 505, 1004
SfoI GGCGCC 1 cut(s) 246
SinI GGWCC 1 cut(s) 490
SmiMI CAYNNNNRTG 2 cut(s) 78, 80
SnaBI TACGTA 1 cut(s) 115
Sse9I AATT 7 cut(s) 165, 187, 318, 447, 576, 879, 1059
SsiI CCGC 1 cut(s) 571
SspDI GGCGCC 1 cut(s) 244
SspI AATATT 1 cut(s) 844
SspMI CTAG 2 cut(s) 387, 635
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 4 cut(s) 342, 363, 492, 874
StyI CCWWGG 3 cut(s) 35, 291, 912
TaaI ACNGT 3 cut(s) 176, 442, 968
TaiI ACGT 5 cut(s) 117, 357, 397, 505, 544
TaqI TCGA 3 cut(s) 134, 414, 644
TasI AATT 7 cut(s) 165, 187, 318, 447, 576, 879, 1059
TfiI GAWTC 1 cut(s) 717
Tru1I TTAA 2 cut(s) 285, 936
Tru9I TTAA 2 cut(s) 285, 936
TscAI CASTG 3 cut(s) 82, 859, 973
TseFI GTSAC 1 cut(s) 71
TseI GCWGC 1 cut(s) 767
Tsp45I GTSAC 1 cut(s) 71
TspDTI ATGAA 7 cut(s) 468, 539, 605, 639, 795, 818, 944
TspGWI ACGGA 2 cut(s) 227, 649
TspRI CASTG 3 cut(s) 82, 859, 973
VpaK11BI GGWCC 1 cut(s) 490
XceI RCATGY 1 cut(s) 476
XcmI CCANNNNNNNNNTGG 3 cut(s) 484, 658, 757
XmnI GAANNNNTTC 1 cut(s) 1076
XspI CTAG 2 cut(s) 387, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.