Belongs to the actin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 682 |
| Acc36I | ACCTGC | 2 cut(s) | 488, 682 |
| AccB1I | GGYRCC | 1 cut(s) | 916 |
| AciI | CCGC | 2 cut(s) | 64, 721 |
| AclWI | GGATC | 1 cut(s) | 978 |
| AcoI | YGGCCR | 1 cut(s) | 61 |
| AcsI | RAATTY | 1 cut(s) | 528 |
| AcuI | CTGAAG | 1 cut(s) | 294 |
| AcyI | GRCGYC | 1 cut(s) | 516 |
| AfaI | GTAC | 5 cut(s) | 353, 397, 735, 965, 1065 |
| AfeI | AGCGCT | 1 cut(s) | 716 |
| AgsI | TTSAA | 2 cut(s) | 337, 638 |
| AjiI | CACGTC | 1 cut(s) | 71 |
| AjnI | CCWGG | 2 cut(s) | 363, 874 |
| AleI | CACNNNNGTG | 2 cut(s) | 78, 80 |
| AluBI | AGCT | 6 cut(s) | 566, 601, 634, 672, 767, 911 |
| AluI | AGCT | 6 cut(s) | 566, 601, 634, 672, 767, 911 |
| Alw26I | GTCTC | 2 cut(s) | 323, 776 |
| AlwI | GGATC | 1 cut(s) | 978 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 972 |
| Ama87I | CYCGRG | 1 cut(s) | 953 |
| Aor51HI | AGCGCT | 1 cut(s) | 716 |
| AoxI | GGCC | 4 cut(s) | 61, 243, 1032, 1051 |
| ApeKI | GCWGC | 3 cut(s) | 723, 767, 924 |
| ApoI | RAATTY | 1 cut(s) | 528 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1076 |
| AspLEI | GCGC | 1 cut(s) | 717 |
| AspS9I | GGNCC | 2 cut(s) | 244, 1052 |
| AsuHPI | GGTGA | 4 cut(s) | 502, 547, 691, 706 |
| AvaI | CYCGRG | 1 cut(s) | 953 |
| BanI | GGYRCC | 1 cut(s) | 916 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 590, 622 |
| BauI | CACGAG | 1 cut(s) | 779 |
| BbvI | GCAGC | 3 cut(s) | 710, 754, 936 |
| BccI | CCATC | 8 cut(s) | 79, 368, 419, 485, 680, 754, 995, 1063 |
| BciT130I | CCWGG | 2 cut(s) | 365, 876 |
| BclI | TGATCA | 1 cut(s) | 696 |
| BcoDI | GTCTC | 2 cut(s) | 323, 776 |
| BfaI | CTAG | 2 cut(s) | 51, 921 |
| BfmI | CTRYAG | 1 cut(s) | 768 |
| BfoI | RGCGCY | 1 cut(s) | 718 |
| BfuAI | ACCTGC | 2 cut(s) | 488, 682 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 365, 688 |
| BisI | GCNGC | 4 cut(s) | 64, 724, 768, 925 |
| BlpI | GCTNAGC | 2 cut(s) | 567, 711 |
| BlsI | GCNGC | 4 cut(s) | 65, 725, 769, 926 |
| BmcAI | AGTACT | 1 cut(s) | 735 |
| Bme1390I | CCNGG | 2 cut(s) | 365, 876 |
| BmeT110I | CYCGRG | 1 cut(s) | 953 |
| BmgBI | CACGTC | 1 cut(s) | 71 |
| BmgT120I | GGNCC | 2 cut(s) | 244, 1052 |
| BmiI | GGNNCC | 2 cut(s) | 246, 918 |
| BmrFI | CCNGG | 2 cut(s) | 365, 876 |
| BmsI | GCATC | 4 cut(s) | 19, 97, 223, 1004 |
| BpmI | CTGGAG | 2 cut(s) | 42, 441 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 567, 711 |
| Bsa29I | ATCGAT | 1 cut(s) | 982 |
| BsaHI | GRCGYC | 1 cut(s) | 516 |
| BsaI | GGTCTC | 1 cut(s) | 776 |
| BsaJI | CCNNGG | 3 cut(s) | 35, 874, 1029 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 414, 444 |
| Bse1I | ACTGG | 4 cut(s) | 82, 260, 387, 406 |
| Bse3DI | GCAATG | 2 cut(s) | 609, 879 |
| BseBI | CCWGG | 2 cut(s) | 365, 876 |
| BseCI | ATCGAT | 1 cut(s) | 982 |
| BseDI | CCNNGG | 3 cut(s) | 35, 874, 1029 |
| BseGI | GGATG | 7 cut(s) | 112, 199, 256, 360, 477, 746, 987 |
| BseMI | GCAATG | 2 cut(s) | 609, 879 |
| BseMII | CTCAG | 4 cut(s) | 143, 558, 702, 865 |
| BseNI | ACTGG | 4 cut(s) | 82, 260, 387, 406 |
| BseX3I | CGGCCG | 1 cut(s) | 61 |
| BseXI | GCAGC | 3 cut(s) | 710, 754, 936 |
| Bsh1285I | CGRYCG | 1 cut(s) | 64 |
| BshFI | GGCC | 4 cut(s) | 63, 245, 1034, 1053 |
| BshNI | GGYRCC | 1 cut(s) | 916 |
| BshVI | ATCGAT | 1 cut(s) | 982 |
| BsiEI | CGRYCG | 1 cut(s) | 64 |
| BsiHKCI | CYCGRG | 1 cut(s) | 953 |
| BslFI | GGGAC | 1 cut(s) | 428 |
| BsmAI | GTCTC | 2 cut(s) | 323, 776 |
| BsmFI | GGGAC | 1 cut(s) | 428 |
| BsmI | GAATGC | 1 cut(s) | 774 |
| BsnI | GGCC | 4 cut(s) | 63, 245, 1034, 1053 |
| Bso31I | GGTCTC | 1 cut(s) | 776 |
| BsoBI | CYCGRG | 1 cut(s) | 953 |
| Bsp1407I | TGTACA | 1 cut(s) | 1063 |
| Bsp143I | GATC | 3 cut(s) | 696, 979, 983 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 567, 711 |
| BspACI | CCGC | 2 cut(s) | 64, 721 |
| BspANI | GGCC | 4 cut(s) | 63, 245, 1034, 1053 |
| BspCNI | CTCAG | 4 cut(s) | 142, 559, 703, 864 |
| BspDI | ATCGAT | 1 cut(s) | 982 |
| BspHI | TCATGA | 1 cut(s) | 801 |
| BspLI | GGNNCC | 2 cut(s) | 246, 918 |
| BspMAI | CTGCAG | 1 cut(s) | 772 |
| BspMI | ACCTGC | 2 cut(s) | 488, 682 |
| BspPI | GGATC | 1 cut(s) | 978 |
| BspQI | GCTCTTC | 2 cut(s) | 471, 662 |
| BspT107I | GGYRCC | 1 cut(s) | 916 |
| BspTNI | GGTCTC | 1 cut(s) | 776 |
| BsrDI | GCAATG | 2 cut(s) | 609, 879 |
| BsrGI | TGTACA | 1 cut(s) | 1063 |
| BsrI | ACTGG | 4 cut(s) | 82, 260, 387, 406 |
| BssECI | CCNNGG | 3 cut(s) | 35, 874, 1029 |
| BssMI | GATC | 3 cut(s) | 696, 979, 983 |
| BssNI | GRCGYC | 1 cut(s) | 516 |
| BssSI | CACGAG | 1 cut(s) | 779 |
| BssT1I | CCWWGG | 2 cut(s) | 35, 1029 |
| Bst2BI | CACGAG | 1 cut(s) | 779 |
| Bst2UI | CCWGG | 2 cut(s) | 365, 876 |
| Bst4CI | ACNGT | 2 cut(s) | 442, 968 |
| Bst6I | CTCTTC | 3 cut(s) | 471, 662, 743 |
| BstACI | GRCGYC | 1 cut(s) | 516 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 611 |
| BstAUI | TGTACA | 1 cut(s) | 1063 |
| BstC8I | GCNNGC | 2 cut(s) | 603, 772 |
| BstDEI | CTNAG | 6 cut(s) | 129, 493, 567, 711, 851, 1001 |
| BstF5I | GGATG | 7 cut(s) | 112, 199, 256, 360, 477, 746, 987 |
| BstH2I | RGCGCY | 1 cut(s) | 718 |
| BstHHI | GCGC | 1 cut(s) | 717 |
| BstKTI | GATC | 3 cut(s) | 699, 982, 986 |
| BstMAI | GTCTC | 2 cut(s) | 323, 776 |
| BstMBI | GATC | 3 cut(s) | 696, 979, 983 |
| BstMCI | CGRYCG | 1 cut(s) | 64 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 17, 38, 60, 365, 611, 688, 723 |
| BstNI | CCWGG | 2 cut(s) | 365, 876 |
| BstSCI | CCNGG | 2 cut(s) | 363, 874 |
| BstSFI | CTRYAG | 1 cut(s) | 768 |
| BstV1I | GCAGC | 3 cut(s) | 710, 754, 936 |
| BstZI | CGGCCG | 1 cut(s) | 61 |
| Bsu15I | ATCGAT | 1 cut(s) | 982 |
| BsuRI | GGCC | 4 cut(s) | 63, 245, 1034, 1053 |
| BsuTUI | ATCGAT | 1 cut(s) | 982 |
| BtrI | CACGTC | 1 cut(s) | 71 |
| BtsCI | GGATG | 7 cut(s) | 112, 199, 256, 360, 477, 746, 987 |
| BtsIMutI | CAGTG | 6 cut(s) | 75, 270, 394, 438, 859, 973 |
| BveI | ACCTGC | 2 cut(s) | 488, 682 |
| Cac8I | GCNNGC | 2 cut(s) | 603, 772 |
| CaiI | CAGNNNCTG | 1 cut(s) | 972 |
| CciI | TCATGA | 1 cut(s) | 801 |
| CfoI | GCGC | 1 cut(s) | 717 |
| Cfr13I | GGNCC | 2 cut(s) | 244, 1052 |
| ClaI | ATCGAT | 1 cut(s) | 982 |
| CseI | GACGC | 1 cut(s) | 524 |
| Csp6I | GTAC | 5 cut(s) | 352, 396, 734, 964, 1064 |
| CviAII | CATG | 7 cut(s) | 173, 322, 473, 751, 802, 868, 928 |
| CviQI | GTAC | 5 cut(s) | 352, 396, 734, 964, 1064 |
| DdeI | CTNAG | 6 cut(s) | 129, 493, 567, 711, 851, 1001 |
| DpnI | GATC | 3 cut(s) | 698, 981, 985 |
| DpnII | GATC | 3 cut(s) | 696, 979, 983 |
| EaeI | YGGCCR | 1 cut(s) | 61 |
| EagI | CGGCCG | 1 cut(s) | 61 |
| Eam1104I | CTCTTC | 3 cut(s) | 471, 662, 743 |
| EarI | CTCTTC | 3 cut(s) | 471, 662, 743 |
| EclXI | CGGCCG | 1 cut(s) | 61 |
| Eco130I | CCWWGG | 2 cut(s) | 35, 1029 |
| Eco31I | GGTCTC | 1 cut(s) | 776 |
| Eco47III | AGCGCT | 1 cut(s) | 716 |
| Eco52I | CGGCCG | 1 cut(s) | 61 |
| Eco57I | CTGAAG | 1 cut(s) | 294 |
| Eco88I | CYCGRG | 1 cut(s) | 953 |
| EcoRII | CCWGG | 2 cut(s) | 363, 874 |
| EcoT14I | CCWWGG | 2 cut(s) | 35, 1029 |
| EcoT22I | ATGCAT | 1 cut(s) | 112 |
| ErhI | CCWWGG | 2 cut(s) | 35, 1029 |
| FaeI | CATG | 7 cut(s) | 176, 325, 476, 754, 805, 871, 931 |
| FaqI | GGGAC | 1 cut(s) | 428 |
| FatI | CATG | 7 cut(s) | 172, 321, 472, 750, 801, 867, 927 |
| FauNDI | CATATG | 1 cut(s) | 112 |
| FbaI | TGATCA | 1 cut(s) | 696 |
| Fnu4HI | GCNGC | 4 cut(s) | 64, 724, 768, 925 |
| FokI | GGATG | 7 cut(s) | 119, 206, 243, 347, 464, 733, 974 |
| Fsp4HI | GCNGC | 4 cut(s) | 64, 724, 768, 925 |
| FspBI | CTAG | 2 cut(s) | 51, 921 |
| GlaI | GCGC | 1 cut(s) | 716 |
| GluI | GCNGC | 4 cut(s) | 64, 724, 768, 925 |
| GsuI | CTGGAG | 2 cut(s) | 42, 441 |
| HaeII | RGCGCY | 1 cut(s) | 718 |
| HaeIII | GGCC | 4 cut(s) | 63, 245, 1034, 1053 |
| HgaI | GACGC | 1 cut(s) | 524 |
| HhaI | GCGC | 1 cut(s) | 717 |
| Hin1I | GRCGYC | 1 cut(s) | 516 |
| Hin1II | CATG | 7 cut(s) | 176, 325, 476, 754, 805, 871, 931 |
| Hin6I | GCGC | 1 cut(s) | 715 |
| HinP1I | GCGC | 1 cut(s) | 715 |
| HindIII | AAGCTT | 1 cut(s) | 599 |
| HinfI | GANTC | 3 cut(s) | 151, 416, 1046 |
| HphI | GGTGA | 4 cut(s) | 502, 547, 691, 706 |
| Hpy166II | GTNNAC | 1 cut(s) | 1066 |
| Hpy188I | TCNGA | 3 cut(s) | 119, 156, 285 |
| Hpy188III | TCNNGA | 9 cut(s) | 148, 302, 413, 420, 488, 503, 779, 802, 973 |
| Hpy8I | GTNNAC | 1 cut(s) | 1066 |
| Hpy99I | CGWCG | 2 cut(s) | 359, 518 |
| HpyAV | CCTTC | 3 cut(s) | 562, 586, 1018 |
| HpyCH4III | ACNGT | 2 cut(s) | 442, 968 |
| HpyCH4IV | ACGT | 3 cut(s) | 70, 139, 354 |
| HpyCH4V | TGCA | 4 cut(s) | 110, 605, 770, 995 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 17, 38, 60, 365, 611, 688, 723 |
| HpyF3I | CTNAG | 6 cut(s) | 129, 493, 567, 711, 851, 1001 |
| HpySE526I | ACGT | 3 cut(s) | 70, 139, 354 |
| Hsp92I | GRCGYC | 1 cut(s) | 516 |
| Hsp92II | CATG | 7 cut(s) | 176, 325, 476, 754, 805, 871, 931 |
| HspAI | GCGC | 1 cut(s) | 715 |
| Ksp22I | TGATCA | 1 cut(s) | 696 |
| Kzo9I | GATC | 3 cut(s) | 696, 979, 983 |
| LguI | GCTCTTC | 2 cut(s) | 471, 662 |
| LmnI | GCTCC | 4 cut(s) | 37, 346, 631, 952 |
| Lsp1109I | GCAGC | 3 cut(s) | 710, 754, 936 |
| LweI | GCATC | 4 cut(s) | 19, 97, 223, 1004 |
| MaeI | CTAG | 2 cut(s) | 51, 921 |
| MaeII | ACGT | 3 cut(s) | 70, 139, 354 |
| MaeIII | GTNAC | 3 cut(s) | 71, 434, 503 |
| MalI | GATC | 3 cut(s) | 698, 981, 985 |
| MboI | GATC | 3 cut(s) | 696, 979, 983 |
| MboII | GAAGA | 6 cut(s) | 217, 263, 458, 648, 679, 730 |
| MfeI | CAATTG | 2 cut(s) | 187, 1059 |
| MluCI | AATT | 4 cut(s) | 187, 528, 576, 1059 |
| MlyI | GAGTC | 2 cut(s) | 145, 410 |
| MmeI | TCCRAC | 2 cut(s) | 70, 97 |
| MnlI | CCTC | 8 cut(s) | 118, 291, 389, 448, 491, 518, 860, 951 |
| Mph1103I | ATGCAT | 1 cut(s) | 112 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1076 |
| MseI | TTAA | 1 cut(s) | 6 |
| MslI | CAYNNNNRTG | 2 cut(s) | 78, 80 |
| MspA1I | CMGCKG | 2 cut(s) | 566, 723 |
| MspR9I | CCNGG | 2 cut(s) | 365, 876 |
| MunI | CAATTG | 2 cut(s) | 187, 1059 |
| Mva1269I | GAATGC | 1 cut(s) | 774 |
| MvaI | CCWGG | 2 cut(s) | 365, 876 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 17, 38, 60, 365, 611, 688, 723 |
| NdeI | CATATG | 1 cut(s) | 112 |
| NdeII | GATC | 3 cut(s) | 696, 979, 983 |
| NlaIII | CATG | 7 cut(s) | 176, 325, 476, 754, 805, 871, 931 |
| NlaIV | GGNNCC | 2 cut(s) | 246, 918 |
| NmeAIII | GCCGAG | 1 cut(s) | 1060 |
| NmuCI | GTSAC | 3 cut(s) | 71, 434, 503 |
| NsiI | ATGCAT | 1 cut(s) | 112 |
| OliI | CACNNNNGTG | 2 cut(s) | 78, 80 |
| PagI | TCATGA | 1 cut(s) | 801 |
| PaqCI | CACCTGC | 1 cut(s) | 682 |
| PciSI | GCTCTTC | 2 cut(s) | 471, 662 |
| PctI | GAATGC | 1 cut(s) | 774 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1076 |
| PfeI | GAWTC | 1 cut(s) | 1046 |
| PkrI | GCNGC | 4 cut(s) | 65, 725, 769, 926 |
| PleI | GAGTC | 2 cut(s) | 145, 410 |
| PpsI | GAGTC | 2 cut(s) | 145, 410 |
| Psp6I | CCWGG | 2 cut(s) | 363, 874 |
| PspGI | CCWGG | 2 cut(s) | 363, 874 |
| PspN4I | GGNNCC | 2 cut(s) | 246, 918 |
| PspPI | GGNCC | 2 cut(s) | 244, 1052 |
| PstI | CTGCAG | 1 cut(s) | 772 |
| PstNI | CAGNNNCTG | 1 cut(s) | 972 |
| PvuII | CAGCTG | 1 cut(s) | 566 |
| RsaI | GTAC | 5 cut(s) | 353, 397, 735, 965, 1065 |
| RsaNI | GTAC | 5 cut(s) | 352, 396, 734, 964, 1064 |
| RseI | CAYNNNNRTG | 2 cut(s) | 78, 80 |
| SapI | GCTCTTC | 2 cut(s) | 471, 662 |
| SaqAI | TTAA | 1 cut(s) | 6 |
| SatI | GCNGC | 4 cut(s) | 64, 724, 768, 925 |
| Sau3AI | GATC | 3 cut(s) | 696, 979, 983 |
| Sau96I | GGNCC | 2 cut(s) | 244, 1052 |
| ScaI | AGTACT | 1 cut(s) | 735 |
| SchI | GAGTC | 2 cut(s) | 145, 410 |
| ScrFI | CCNGG | 2 cut(s) | 365, 876 |
| SfaNI | GCATC | 4 cut(s) | 19, 97, 223, 1004 |
| SfcI | CTRYAG | 1 cut(s) | 768 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 78, 80 |
| Sse9I | AATT | 4 cut(s) | 187, 528, 576, 1059 |
| SsiI | CCGC | 2 cut(s) | 64, 721 |
| SspMI | CTAG | 2 cut(s) | 51, 921 |
| StyD4I | CCNGG | 2 cut(s) | 363, 874 |
| StyI | CCWWGG | 2 cut(s) | 35, 1029 |
| TaaI | ACNGT | 2 cut(s) | 442, 968 |
| TaiI | ACGT | 3 cut(s) | 73, 142, 357 |
| TaqI | TCGA | 3 cut(s) | 414, 816, 982 |
| TaqII | GACCGA | 1 cut(s) | 551 |
| TasI | AATT | 4 cut(s) | 187, 528, 576, 1059 |
| TatI | WGTACW | 3 cut(s) | 733, 963, 1063 |
| TauI | GCSGC | 1 cut(s) | 66 |
| TfiI | GAWTC | 1 cut(s) | 1046 |
| Tru1I | TTAA | 1 cut(s) | 6 |
| Tru9I | TTAA | 1 cut(s) | 6 |
| TscAI | CASTG | 6 cut(s) | 82, 277, 394, 445, 859, 973 |
| TseFI | GTSAC | 3 cut(s) | 71, 434, 503 |
| TseI | GCWGC | 3 cut(s) | 723, 767, 924 |
| Tsp45I | GTSAC | 3 cut(s) | 71, 434, 503 |
| TspDTI | ATGAA | 7 cut(s) | 246, 539, 605, 639, 818, 944, 1059 |
| TspGWI | ACGGA | 1 cut(s) | 473 |
| TspRI | CASTG | 6 cut(s) | 82, 277, 394, 445, 859, 973 |
| XapI | RAATTY | 1 cut(s) | 528 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 757 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1076 |
| XspI | CTAG | 2 cut(s) | 51, 921 |
| ZrmI | AGTACT | 1 cut(s) | 735 |
| Zsp2I | ATGCAT | 1 cut(s) | 112 |