Rw5G039710

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000536
Physical Location & Seq
Forward (+)
70146482 .. 70148888
2407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G039710.1

Sequence Viewer

Length: 1134 bp
ATGGCTGAGGCTGAGGACATTCAACCACTTGTCTGTGACAATGGGACTGGGATGGTTAAGGCTGGATTTGCTGGAGATGATGCTCCAAGGGCTGTCTTTCCTAGCATTGTTGGCCGCCCACGTCACACTGGTGTGATGGTTGGCATGGGTCAAAAGGATGCATATGTTGGAGACGAGGCTCAGTCTAAGCGTGGTATCTTGACCCTGAAATACCCCATTGAACATGGTATTGTGAGCAACTGGGATGATATGGAGAAAATTTGGCATCATACTTTCTACAATGAACTTCGTGTTGCCCCAGAAGAACATCCAGTTCTACTCACAGAAGCACCTCTGAACCCAAAGGCCAATCGTGAGAAAATGACCCAAATCATGTTTGAGACATTCAACACTCCTGCCATGTATGTCGCCATCCAGGCTGTTCTTTCCCTCTATGCCAGTGGTCGTACAACTGGTATTGTCCTGGACTCTGGAGATGGTGTCAGCCACACTGTTCCCATTTATGAGGGGTATGCTCTCCCACATGCCATTCTCCGTCTTGACTTAGCAGGTCGGGACCTCACTGATGCTCTTATGAAAATCTTGACTGAGCGTGGCTATTCCTTCACCACCACAGCTGAGCGAGAAATAGTCAGGGACATGAAGGAGAAGCTTGCATATCTTGCTCTCGACTATGAGCAGGAGCTTGAAACTGCTAAGACAAGTTCTTCAGTTGAGAAAAGCTATGAATTGCCTGATGGACAGGTGATCACCATCGGTGCTGAGAGATTCCGTTGCCCAGAAGTACTTTTCCAACCATCTATGATTGGAATGGAAGCTGCAGGCATTCATGAGACTACATACAACTCTATCATGAAGTGTGATGTCGATATCAGGAAGGATTTGTATGGAAACATTGTTCTCAGTGGTGGGTCTACCATGTTCCCTGGCATTGCTGACAGAATGAGCAAGGAAATCACGGCTTTGGCACCAAGCAGCATGAAAATCAAGGTTGTTGCTCCACCTGAGAGGAAGTACAGTGTCTGGATTGGTGGATCCATCCTTGCATCTCTAAGCACTTTCCAGCAGATGTGGATCGCAAAGGCAGAGTATGATGAATCTGGTCCATCAATTGTACACAGGAAGTGTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

377

Amino Acids

41.68

Weight (kDa)

5.31

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 5 - 377 1.1e-145 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000536)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37620 AT2G37620 AT2G37620 AT2G37620 AT3G46520 AT3G46520 AT3G53750 AT3G53750 AT5G59370 AT5G59370
fragaria_vesca FvH4_3g35960 FvH4_6g13940 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_7g22410 FvH4_7g22410 FvH4_7g22410
malus_domestica MD03G1095300.v1.1 MD04G1127400.v1.1 MD11G1110300.v1.1 MD12G1056900.v1.1 MD12G1140800.v1.1 MD14G1056700.v1.1
prunus_persica Prupe.6G078800_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.7G077300_v2.0.a1 Prupe.7G077300_v2.0.a1
pyrus_communis pycom01g02640 pycom03g07780 pycom11g09110 pycom126g00040 pycom14g04650 pycom15g30330
rosa_chinensis RchiOBHm_Chr1g0367041 RchiOBHm_Chr3g0466761 RchiOBHm_Chr3g0479651 RchiOBHm_Chr5g0064311
rosa_laevigata RLG00000023550 RLG00000024555 RLG00000027304 RLG00000035715
rosa_multiflora Rmu_co8518979.1_g000001 Rmu_sc0002269.1_g000004 Rmu_sc0003467.1_g000022 Rmu_sc0003755.1_g000002 Rmu_sc0013440.1_g000001 Rmu_sc0013700.1_g000002 Rmu_sc0026659.1_g000001 Rmu_sc0037142.1_g000001
rosa_roxburghii Rroxscaffold_1G00016700 Rroxscaffold_4G00289680 Rroxscaffold_6G00402090
rosa_rugosa Rorug01G0334100 Rorug03G0083700 Rorug03G0083800 Rorug03G0177100 Rorug03G0177200 Rorug05G0364000 Rorug05G0364100
rosa_samantha Rh1AG340900 Rh1BG301400 Rh1CG317600 Rh1DG333800 Rh2AG276500 Rh3AG130800 Rh3AG227900 Rh3BG151800 Rh3BG261100 Rh3CG151700 Rh3DG152500 Rh3DG254400 Rh5AG421900 Rh5BG437600 Rh5CG460300 Rh5DG450700
rosa_wichuraiana Rw0G023870 Rw1G030280 Rw3G012360 Rw3G020390 Rw5G039710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 539
AccB1I GGYRCC 1 cut(s) 967
AccI GTMKAC 1 cut(s) 914
AciI CCGC 1 cut(s) 115
AclWI GGATC 3 cut(s) 1029, 1042, 1082
AcoI YGGCCR 1 cut(s) 112
AcsI RAATTY 1 cut(s) 258
AcuI CTGAAG 1 cut(s) 693
AfaI GTAC 4 cut(s) 448, 786, 1016, 1116
AgsI TTSAA 4 cut(s) 23, 221, 388, 689
AjiI CACGTC 1 cut(s) 122
AjnI CCWGG 3 cut(s) 414, 462, 925
AleI CACNNNNGTG 2 cut(s) 129, 131
AloI GAACNNNNNNTCC 2 cut(s) 882, 914
AluBI AGCT 5 cut(s) 617, 652, 685, 723, 818
AluI AGCT 5 cut(s) 617, 652, 685, 723, 818
Alw26I GTCTC 3 cut(s) 165, 374, 827
AlwI GGATC 3 cut(s) 1029, 1042, 1082
AlwNI CAGNNNCTG 1 cut(s) 1023
AoxI GGCC 2 cut(s) 112, 345
ApeKI GCWGC 2 cut(s) 818, 975
ApoI RAATTY 1 cut(s) 258
Asp700I GAANNNNTTC 1 cut(s) 1127
AspS9I GGNCC 2 cut(s) 556, 1103
AsuHPI GGTGA 3 cut(s) 598, 742, 757
AvaII GGWCC 2 cut(s) 556, 1103
BamHI GGATCC 1 cut(s) 1034
BanI GGYRCC 1 cut(s) 967
BbvCI CCTCAGC 2 cut(s) 6, 12
BbvI GCAGC 2 cut(s) 805, 987
BccI CCATC 9 cut(s) 46, 130, 419, 470, 731, 761, 805, 1046, 1114
BceAI ACGGC 1 cut(s) 975
BciT130I CCWGG 3 cut(s) 416, 464, 927
BclI TGATCA 1 cut(s) 747
BcoDI GTCTC 3 cut(s) 165, 374, 827
BfaI CTAG 2 cut(s) 102, 1132
BfmI CTRYAG 1 cut(s) 819
BfuAI ACCTGC 1 cut(s) 539
BglI GCCNNNNNGGC 1 cut(s) 416
BisI GCNGC 3 cut(s) 115, 819, 976
BlpI GCTNAGC 1 cut(s) 618
BlsI GCNGC 3 cut(s) 116, 820, 977
BmcAI AGTACT 1 cut(s) 786
Bme1390I CCNGG 3 cut(s) 416, 464, 927
Bme18I GGWCC 2 cut(s) 556, 1103
BmgBI CACGTC 1 cut(s) 122
BmgT120I GGNCC 2 cut(s) 556, 1103
BmiI GGNNCC 3 cut(s) 557, 969, 1036
BmrFI CCNGG 3 cut(s) 416, 464, 927
BmrI ACTGGG 2 cut(s) 57, 250
BmsI GCATC 5 cut(s) 70, 148, 274, 556, 1055
BmuI ACTGGG 2 cut(s) 57, 250
BpmI CTGGAG 2 cut(s) 93, 492
Bpu10I CCTNAGC 2 cut(s) 6, 12
Bpu1102I GCTNAGC 1 cut(s) 618
BsaBI GATNNNNATC 2 cut(s) 752, 1073
BsaJI CCNNGG 2 cut(s) 86, 925
BsaXI ACNNNNNCTCC 2 cut(s) 465, 495
Bse1I ACTGG 6 cut(s) 52, 133, 245, 311, 438, 457
Bse3DI GCAATG 1 cut(s) 930
Bse8I GATNNNNATC 2 cut(s) 752, 1073
BseBI CCWGG 3 cut(s) 416, 464, 927
BseDI CCNNGG 2 cut(s) 86, 925
BseGI GGATG 6 cut(s) 57, 163, 250, 307, 411, 1038
BseJI GATNNNNATC 2 cut(s) 752, 1073
BseMI GCAATG 1 cut(s) 930
BseMII CTCAG 6 cut(s) 194, 579, 609, 753, 916, 996
BseNI ACTGG 6 cut(s) 52, 133, 245, 311, 438, 457
BseXI GCAGC 2 cut(s) 805, 987
BshFI GGCC 2 cut(s) 114, 347
BshNI GGYRCC 1 cut(s) 967
BslFI GGGAC 3 cut(s) 58, 569, 650
BsmAI GTCTC 3 cut(s) 165, 374, 827
BsmBI CGTCTC 1 cut(s) 165
BsmFI GGGAC 3 cut(s) 58, 569, 650
BsmI GAATGC 1 cut(s) 825
BsnI GGCC 2 cut(s) 114, 347
Bsp1407I TGTACA 1 cut(s) 1114
Bsp143I GATC 3 cut(s) 747, 1034, 1074
Bsp1720I GCTNAGC 1 cut(s) 618
BspACI CCGC 1 cut(s) 115
BspANI GGCC 2 cut(s) 114, 347
BspCNI CTCAG 7 cut(s) 4, 193, 580, 610, 754, 915, 997
BspHI TCATGA 2 cut(s) 829, 852
BspLI GGNNCC 3 cut(s) 557, 969, 1036
BspMAI CTGCAG 1 cut(s) 823
BspMI ACCTGC 1 cut(s) 539
BspPI GGATC 3 cut(s) 1029, 1042, 1082
BspT107I GGYRCC 1 cut(s) 967
BsrDI GCAATG 1 cut(s) 930
BsrGI TGTACA 1 cut(s) 1114
BsrI ACTGG 6 cut(s) 52, 133, 245, 311, 438, 457
BssECI CCNNGG 2 cut(s) 86, 925
BssMI GATC 3 cut(s) 747, 1034, 1074
BssT1I CCWWGG 1 cut(s) 86
Bst2UI CCWGG 3 cut(s) 416, 464, 927
Bst4CI ACNGT 2 cut(s) 493, 1019
BstAPI GCANNNNNTGC 1 cut(s) 662
BstAUI TGTACA 1 cut(s) 1114
BstC8I GCNNGC 2 cut(s) 654, 823
BstF5I GGATG 6 cut(s) 57, 163, 250, 307, 411, 1038
BstKTI GATC 3 cut(s) 750, 1037, 1077
BstMAI GTCTC 3 cut(s) 165, 374, 827
BstMBI GATC 3 cut(s) 747, 1034, 1074
BstMWI GCNNNNNNNGC 5 cut(s) 68, 89, 111, 416, 662
BstNI CCWGG 3 cut(s) 416, 464, 927
BstNSI RCATGY 1 cut(s) 527
BstSCI CCNGG 3 cut(s) 414, 462, 925
BstSFI CTRYAG 1 cut(s) 819
BstV1I GCAGC 2 cut(s) 805, 987
BstX2I RGATCY 1 cut(s) 1034
BstYI RGATCY 1 cut(s) 1034
BsuRI GGCC 2 cut(s) 114, 347
BtrI CACGTC 1 cut(s) 122
BtsCI GGATG 6 cut(s) 57, 163, 250, 307, 411, 1038
BtsIMutI CAGTG 6 cut(s) 126, 445, 489, 561, 910, 1024
BveI ACCTGC 1 cut(s) 539
Cac8I GCNNGC 2 cut(s) 654, 823
CaiI CAGNNNCTG 1 cut(s) 1023
CciI TCATGA 2 cut(s) 829, 852
Cfr13I GGNCC 2 cut(s) 556, 1103
Csp6I GTAC 4 cut(s) 447, 785, 1015, 1115
CviQI GTAC 4 cut(s) 447, 785, 1015, 1115
DpnI GATC 3 cut(s) 749, 1036, 1076
DpnII GATC 3 cut(s) 747, 1034, 1074
EaeI YGGCCR 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 86
Eco32I GATATC 1 cut(s) 871
Eco47I GGWCC 2 cut(s) 556, 1103
Eco57I CTGAAG 1 cut(s) 693
EcoO109I RGGNCCY 1 cut(s) 556
EcoRII CCWGG 3 cut(s) 414, 462, 925
EcoRV GATATC 1 cut(s) 871
EcoT14I CCWWGG 1 cut(s) 86
EcoT22I ATGCAT 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 86
Esp3I CGTCTC 1 cut(s) 165
FaqI GGGAC 3 cut(s) 58, 569, 650
FauNDI CATATG 1 cut(s) 163
FbaI TGATCA 1 cut(s) 747
FblI GTMKAC 1 cut(s) 914
Fnu4HI GCNGC 3 cut(s) 115, 819, 976
FokI GGATG 6 cut(s) 64, 170, 257, 294, 398, 1025
Fsp4HI GCNGC 3 cut(s) 115, 819, 976
FspBI CTAG 2 cut(s) 102, 1132
GluI GCNGC 3 cut(s) 115, 819, 976
GsuI CTGGAG 2 cut(s) 93, 492
HaeIII GGCC 2 cut(s) 114, 347
HindIII AAGCTT 1 cut(s) 650
HinfI GANTC 3 cut(s) 467, 768, 1097
HphI GGTGA 3 cut(s) 598, 742, 757
Hpy166II GTNNAC 2 cut(s) 915, 1117
Hpy188I TCNGA 1 cut(s) 336
Hpy8I GTNNAC 2 cut(s) 915, 1117
HpyAV CCTTC 3 cut(s) 613, 637, 871
HpyCH4III ACNGT 2 cut(s) 493, 1019
HpyCH4IV ACGT 1 cut(s) 121
HpyCH4V TGCA 4 cut(s) 161, 656, 821, 1046
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 89, 111, 416, 662
HpySE526I ACGT 1 cut(s) 121
Ksp22I TGATCA 1 cut(s) 747
Kzo9I GATC 3 cut(s) 747, 1034, 1074
LmnI GCTCC 3 cut(s) 88, 682, 1003
Lsp1109I GCAGC 2 cut(s) 805, 987
LweI GCATC 5 cut(s) 70, 148, 274, 556, 1055
MaeI CTAG 2 cut(s) 102, 1132
MaeII ACGT 1 cut(s) 121
MaeIII GTNAC 2 cut(s) 35, 122
MalI GATC 3 cut(s) 749, 1036, 1076
MboI GATC 3 cut(s) 747, 1034, 1074
MboII GAAGA 2 cut(s) 314, 699
MfeI CAATTG 1 cut(s) 1110
MflI RGATCY 1 cut(s) 1034
MluCI AATT 3 cut(s) 258, 728, 1110
MlyI GAGTC 1 cut(s) 461
MmeI TCCRAC 2 cut(s) 148, 817
MnlI CCTC 7 cut(s) 7, 169, 342, 440, 499, 569, 1002
Mph1103I ATGCAT 1 cut(s) 163
MroXI GAANNNNTTC 1 cut(s) 1127
MseI TTAA 1 cut(s) 57
MslI CAYNNNNRTG 2 cut(s) 129, 131
MspA1I CMGCKG 1 cut(s) 617
MspR9I CCNGG 3 cut(s) 416, 464, 927
MunI CAATTG 1 cut(s) 1110
Mva1269I GAATGC 1 cut(s) 825
MvaI CCWGG 3 cut(s) 416, 464, 927
MwoI GCNNNNNNNGC 5 cut(s) 68, 89, 111, 416, 662
NdeI CATATG 1 cut(s) 163
NdeII GATC 3 cut(s) 747, 1034, 1074
NlaIV GGNNCC 3 cut(s) 557, 969, 1036
NmuCI GTSAC 2 cut(s) 35, 122
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 1 cut(s) 527
OliI CACNNNNGTG 2 cut(s) 129, 131
PagI TCATGA 2 cut(s) 829, 852
PctI GAATGC 1 cut(s) 825
PdmI GAANNNNTTC 1 cut(s) 1127
PfeI GAWTC 2 cut(s) 768, 1097
PfoI TCCNGGA 1 cut(s) 462
PkrI GCNGC 3 cut(s) 116, 820, 977
PleI GAGTC 1 cut(s) 461
PpsI GAGTC 1 cut(s) 461
PpuMI RGGWCCY 1 cut(s) 556
Psp5II RGGWCCY 1 cut(s) 556
Psp6I CCWGG 3 cut(s) 414, 462, 925
PspGI CCWGG 3 cut(s) 414, 462, 925
PspN4I GGNNCC 3 cut(s) 557, 969, 1036
PspPI GGNCC 2 cut(s) 556, 1103
PspPPI RGGWCCY 1 cut(s) 556
PstI CTGCAG 1 cut(s) 823
PstNI CAGNNNCTG 1 cut(s) 1023
PsuI RGATCY 1 cut(s) 1034
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 4 cut(s) 448, 786, 1016, 1116
RsaNI GTAC 4 cut(s) 447, 785, 1015, 1115
RseI CAYNNNNRTG 2 cut(s) 129, 131
SaqAI TTAA 1 cut(s) 57
SatI GCNGC 3 cut(s) 115, 819, 976
Sau3AI GATC 3 cut(s) 747, 1034, 1074
Sau96I GGNCC 2 cut(s) 556, 1103
ScaI AGTACT 1 cut(s) 786
SchI GAGTC 1 cut(s) 461
ScrFI CCNGG 3 cut(s) 416, 464, 927
SfaNI GCATC 5 cut(s) 70, 148, 274, 556, 1055
SfcI CTRYAG 1 cut(s) 819
SinI GGWCC 2 cut(s) 556, 1103
SmiMI CAYNNNNRTG 2 cut(s) 129, 131
Sse9I AATT 3 cut(s) 258, 728, 1110
SsiI CCGC 1 cut(s) 115
SspMI CTAG 2 cut(s) 102, 1132
StyD4I CCNGG 3 cut(s) 414, 462, 925
StyI CCWWGG 1 cut(s) 86
TaaI ACNGT 2 cut(s) 493, 1019
TaiI ACGT 1 cut(s) 124
TaqI TCGA 2 cut(s) 669, 867
TasI AATT 3 cut(s) 258, 728, 1110
TatI WGTACW 3 cut(s) 784, 1014, 1114
TauI GCSGC 1 cut(s) 117
TfiI GAWTC 2 cut(s) 768, 1097
Tru1I TTAA 1 cut(s) 57
Tru9I TTAA 1 cut(s) 57
TscAI CASTG 6 cut(s) 133, 445, 496, 568, 910, 1024
TseFI GTSAC 2 cut(s) 35, 122
TseI GCWGC 2 cut(s) 818, 975
Tsp45I GTSAC 2 cut(s) 35, 122
TspDTI ATGAA 8 cut(s) 297, 590, 656, 741, 818, 869, 995, 1110
TspGWI ACGGA 2 cut(s) 524, 761
TspRI CASTG 6 cut(s) 133, 445, 496, 568, 910, 1024
VpaK11BI GGWCC 2 cut(s) 556, 1103
XapI RAATTY 1 cut(s) 258
XceI RCATGY 1 cut(s) 527
XmiI GTMKAC 1 cut(s) 914
XmnI GAANNNNTTC 1 cut(s) 1127
XspI CTAG 2 cut(s) 102, 1132
ZrmI AGTACT 1 cut(s) 786
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.