Rroxscaffold_6G00402090

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000536
Physical Location & Seq
Reverse (-)
24470246 .. 24473259
3014 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00402090.1

Sequence Viewer

Length: 1134 bp
ATGGCAGAAAGCGAGGATATTCAGCCCCTTGTTTGTGACAATGGTACCGGAATGGTCAAGGCAGGATTTGCTGGAGATGATGCTCCAAGAGCTGTGTTTCCCAGCATTGTAGGGCGCCCTCGCCACACGGGTGTGATGGTTGGGATGGGACAGAAGGATGCCTATGTGGGGGATGAGGCTCAATCGAAGAGAGGTATTCTGACTTTGAAATACCCGATCGAGCATGGTATTGTTAGCAATTGGGATGATATGGAGAAGATCTGGCATCACACATTCTACAATGAGCTCCGTGTGGCTCCAGAAGAGCATCCAGTTCTCCTCACAGAAGCACCCCTTAACCCCAAGGCCAATCGTGAGAAAATGACTCAAATTATGTTTGAGACATTCAACACTCCTGCTATGTACGTTGCAATCCAGGCCGTCCTTTCCCTGTATGCTAGTGGACGTACAACTGGTATTGTTCTTGACTCTGGGGATGGTGTGAGTCACACAGTTCCGATATATGAAGGGTATGCCCTCCCACATGCCATCCTTCGTTTGGACCTGGCAGGTCGTGATCTTACAGATGCCCTCATGAAAATCTTGACTGAACGTGGTTACTCTTTCACCACTACTGCTGAGCGGGAAATTGTGAGAGACATGAAGGAGAAATTAGCCTATATTGCTCTAGACTATGAACAAGAACTAGAGACAGCGAAGACCAGCTCATCTGTTGAGAAGAGTTATGAATTACCTGATGGGCAGGTTATCACGATTGGTGCTGAAAGATTCCGATGCCCTGAAGTCCTCTTCCAGCCATCCATGATCGGGATGGAAGCTGCTGGTATCCATGAAACCACATACAACTCTATCATGAAGTGTGATGTTGATATCAGGAAGGATCTCTATGGCAACATTGTTCTTTCCGGAGGCTCCACTATGTTCCCTGGAATTGCTGATAGGATGAGCAAAGAGATTACAGCATTAGCTCCAAGTAGCATGAAAATCAAGGTCGTAGCACCGCCCGAGAGGAAATACAGTGTCTGGATAGGAGGCTCCATTTTGGCATCCCTCAGCACCTTCCAACAGATGTGGATTGCAAAGGCTGAGTATGATGAGTCTGGCCCCTCAATTGTTCACAGGAAATGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

377

Amino Acids

41.7

Weight (kDa)

5.31

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 5 - 377 2.9e-146 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000536)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37620 AT2G37620 AT2G37620 AT2G37620 AT3G46520 AT3G46520 AT3G53750 AT3G53750 AT5G59370 AT5G59370
fragaria_vesca FvH4_3g35960 FvH4_6g13940 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_7g22410 FvH4_7g22410 FvH4_7g22410
malus_domestica MD03G1095300.v1.1 MD04G1127400.v1.1 MD11G1110300.v1.1 MD12G1056900.v1.1 MD12G1140800.v1.1 MD14G1056700.v1.1
prunus_persica Prupe.6G078800_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.7G077300_v2.0.a1 Prupe.7G077300_v2.0.a1
pyrus_communis pycom01g02640 pycom03g07780 pycom11g09110 pycom126g00040 pycom14g04650 pycom15g30330
rosa_chinensis RchiOBHm_Chr1g0367041 RchiOBHm_Chr3g0466761 RchiOBHm_Chr3g0479651 RchiOBHm_Chr5g0064311
rosa_laevigata RLG00000023550 RLG00000024555 RLG00000027304 RLG00000035715
rosa_multiflora Rmu_co8518979.1_g000001 Rmu_sc0002269.1_g000004 Rmu_sc0003467.1_g000022 Rmu_sc0003755.1_g000002 Rmu_sc0013440.1_g000001 Rmu_sc0013700.1_g000002 Rmu_sc0026659.1_g000001 Rmu_sc0037142.1_g000001
rosa_roxburghii Rroxscaffold_1G00016700 Rroxscaffold_4G00289680 Rroxscaffold_6G00402090
rosa_rugosa Rorug01G0334100 Rorug03G0083700 Rorug03G0083800 Rorug03G0177100 Rorug03G0177200 Rorug05G0364000 Rorug05G0364100
rosa_samantha Rh1AG340900 Rh1BG301400 Rh1CG317600 Rh1DG333800 Rh2AG276500 Rh3AG130800 Rh3AG227900 Rh3BG151800 Rh3BG261100 Rh3CG151700 Rh3DG152500 Rh3DG254400 Rh5AG421900 Rh5BG437600 Rh5CG460300 Rh5DG450700
rosa_wichuraiana Rw0G023870 Rw1G030280 Rw3G012360 Rw3G020390 Rw5G039710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 539, 733
Acc65I GGTACC 1 cut(s) 44
AccB1I GGYRCC 2 cut(s) 44, 114
AccBSI CCGCTC 1 cut(s) 622
AccIII TCCGGA 1 cut(s) 905
AciI CCGC 2 cut(s) 622, 1001
AclWI GGATC 1 cut(s) 888
AcuI CTGAAG 1 cut(s) 801
AcyI GRCGYC 1 cut(s) 115
AfaI GTAC 3 cut(s) 46, 404, 448
AfiI CCNNNNNNNGG 3 cut(s) 168, 538, 807
AgsI TTSAA 2 cut(s) 208, 388
AjnI CCWGG 3 cut(s) 414, 543, 925
AleI CACNNNNGTG 2 cut(s) 129, 131
AluBI AGCT 5 cut(s) 92, 286, 705, 818, 968
AluI AGCT 5 cut(s) 92, 286, 705, 818, 968
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 3 cut(s) 374, 630, 683
AlwI GGATC 1 cut(s) 888
AlwNI CAGNNNCTG 1 cut(s) 1023
Ama87I CYCGRG 1 cut(s) 1004
Aor13HI TCCGGA 1 cut(s) 905
AoxI GGCC 3 cut(s) 345, 417, 1102
ApeKI GCWGC 1 cut(s) 818
Asp700I GAANNNNTTC 1 cut(s) 1127
Asp718I GGTACC 1 cut(s) 44
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 2 cut(s) 541, 1103
AsuHPI GGTGA 1 cut(s) 598
AvaI CYCGRG 1 cut(s) 1004
AvaII GGWCC 1 cut(s) 541
BaeI ACNNNNGTAYC 2 cut(s) 28, 61
BanI GGYRCC 2 cut(s) 44, 114
BanII GRGCYC 1 cut(s) 288
BbsI GAAGAC 1 cut(s) 704
Bbv12I GWGCWC 1 cut(s) 288
BbvCI CCTCAGC 1 cut(s) 1052
BbvI GCAGC 1 cut(s) 805
BccI CCATC 7 cut(s) 130, 139, 470, 536, 731, 805, 805
BceAI ACGGC 1 cut(s) 404
BciT130I CCWGG 3 cut(s) 416, 545, 927
BciVI GTATCC 1 cut(s) 836
BcoDI GTCTC 3 cut(s) 374, 630, 683
BfaI CTAG 3 cut(s) 438, 668, 686
BfoI RGCGCY 1 cut(s) 118
BfuAI ACCTGC 2 cut(s) 539, 733
BfuI GTATCC 1 cut(s) 836
BglII AGATCT 1 cut(s) 258
BisI GCNGC 1 cut(s) 819
BlpI GCTNAGC 1 cut(s) 618
BlsI GCNGC 1 cut(s) 820
Bme1390I CCNGG 3 cut(s) 416, 545, 927
Bme18I GGWCC 1 cut(s) 541
BmeT110I CYCGRG 1 cut(s) 1004
BmgT120I GGNCC 2 cut(s) 541, 1103
BmiI GGNNCC 6 cut(s) 46, 116, 297, 913, 1036, 1105
BmrFI CCNGG 3 cut(s) 416, 545, 927
BmsI GCATC 7 cut(s) 70, 148, 274, 316, 556, 764, 1055
BpiI GAAGAC 1 cut(s) 704
BpmI CTGGAG 2 cut(s) 93, 282
Bpu10I CCTNAGC 1 cut(s) 1052
Bpu1102I GCTNAGC 1 cut(s) 618
BsaHI GRCGYC 1 cut(s) 115
BsaJI CCNNGG 2 cut(s) 342, 925
BsaWI WCCGGW 2 cut(s) 47, 905
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 538, 807
Bse1I ACTGG 2 cut(s) 311, 457
BseAI TCCGGA 1 cut(s) 905
BseBI CCWGG 3 cut(s) 416, 545, 927
BseDI CCNNGG 2 cut(s) 342, 925
BseLI CCNNNNNNNGG 3 cut(s) 168, 538, 807
BseMII CTCAG 3 cut(s) 609, 1066, 1077
BseNI ACTGG 2 cut(s) 311, 457
BseRI GAGGAG 1 cut(s) 308
BseXI GCAGC 1 cut(s) 805
BseYI CCCAGC 1 cut(s) 101
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 3 cut(s) 347, 419, 1104
BshNI GGYRCC 2 cut(s) 44, 114
BsiEI CGRYCG 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 288
BsiHKCI CYCGRG 1 cut(s) 1004
BsiSI CCGG 2 cut(s) 48, 906
BslFI GGGAC 1 cut(s) 162
BslI CCNNNNNNNGG 3 cut(s) 168, 538, 807
BsmAI GTCTC 3 cut(s) 374, 630, 683
BsmFI GGGAC 1 cut(s) 162
BsnI GGCC 3 cut(s) 347, 419, 1104
BsoBI CYCGRG 1 cut(s) 1004
Bsp1286I GDGCHC 1 cut(s) 288
Bsp13I TCCGGA 1 cut(s) 905
Bsp143I GATC 5 cut(s) 216, 258, 556, 804, 880
Bsp1720I GCTNAGC 1 cut(s) 618
BspACI CCGC 2 cut(s) 622, 1001
BspANI GGCC 3 cut(s) 347, 419, 1104
BspCNI CTCAG 3 cut(s) 610, 1065, 1078
BspEI TCCGGA 1 cut(s) 905
BspHI TCATGA 2 cut(s) 573, 852
BspLI GGNNCC 6 cut(s) 46, 116, 297, 913, 1036, 1105
BspMI ACCTGC 2 cut(s) 539, 733
BspPI GGATC 1 cut(s) 888
BspQI GCTCTTC 1 cut(s) 297
BspT107I GGYRCC 2 cut(s) 44, 114
BsrBI CCGCTC 1 cut(s) 622
BsrI ACTGG 2 cut(s) 311, 457
BssECI CCNNGG 2 cut(s) 342, 925
BssMI GATC 5 cut(s) 216, 258, 556, 804, 880
BssNI GRCGYC 1 cut(s) 115
BssT1I CCWWGG 1 cut(s) 342
Bst2UI CCWGG 3 cut(s) 416, 545, 927
Bst4CI ACNGT 2 cut(s) 493, 1019
Bst6I CTCTTC 4 cut(s) 182, 297, 713, 794
BstACI GRCGYC 1 cut(s) 115
BstAPI GCANNNNNTGC 1 cut(s) 68
BstDEI CTNAG 3 cut(s) 618, 1052, 1086
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 5 cut(s) 219, 261, 559, 807, 883
BstMAI GTCTC 3 cut(s) 374, 630, 683
BstMBI GATC 5 cut(s) 216, 258, 556, 804, 880
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
BstNI CCWGG 3 cut(s) 416, 545, 927
BstNSI RCATGY 1 cut(s) 527
BstSCI CCNGG 3 cut(s) 414, 543, 925
BstV1I GCAGC 1 cut(s) 805
BstV2I GAAGAC 1 cut(s) 704
BstX2I RGATCY 2 cut(s) 258, 880
BstYI RGATCY 2 cut(s) 258, 880
BsuI GTATCC 1 cut(s) 836
BsuRI GGCC 3 cut(s) 347, 419, 1104
BtsIMutI CAGTG 1 cut(s) 1024
BveI ACCTGC 2 cut(s) 539, 733
CaiI CAGNNNCTG 1 cut(s) 1023
CciI TCATGA 2 cut(s) 573, 852
CfoI GCGC 1 cut(s) 117
Cfr13I GGNCC 2 cut(s) 541, 1103
Csp6I GTAC 3 cut(s) 45, 403, 447
CspCI CAANNNNNGTGG 2 cut(s) 1052, 1087
CviAII CATG 8 cut(s) 224, 524, 574, 640, 802, 830, 853, 979
CviQI GTAC 3 cut(s) 45, 403, 447
DdeI CTNAG 3 cut(s) 618, 1052, 1086
DinI GGCGCC 1 cut(s) 116
DpnI GATC 5 cut(s) 218, 260, 558, 806, 882
DpnII GATC 5 cut(s) 216, 258, 556, 804, 880
Eam1104I CTCTTC 4 cut(s) 182, 297, 713, 794
EarI CTCTTC 4 cut(s) 182, 297, 713, 794
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 342
Eco24I GRGCYC 1 cut(s) 288
Eco32I GATATC 1 cut(s) 871
Eco47I GGWCC 1 cut(s) 541
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 1 cut(s) 801
Eco88I CYCGRG 1 cut(s) 1004
EcoICRI GAGCTC 1 cut(s) 286
EcoRII CCWGG 3 cut(s) 414, 543, 925
EcoRV GATATC 1 cut(s) 871
EcoT14I CCWWGG 1 cut(s) 342
EcoT38I GRGCYC 1 cut(s) 288
EgeI GGCGCC 1 cut(s) 116
EheI GGCGCC 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 342
FaeI CATG 8 cut(s) 227, 527, 577, 643, 805, 833, 856, 982
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FaqI GGGAC 1 cut(s) 162
FatI CATG 8 cut(s) 223, 523, 573, 639, 801, 829, 852, 978
FauI CCCGC 1 cut(s) 615
Fnu4HI GCNGC 1 cut(s) 819
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 1 cut(s) 819
FspBI CTAG 3 cut(s) 438, 668, 686
GlaI GCGC 1 cut(s) 116
GluI GCNGC 1 cut(s) 819
GsaI CCCAGC 1 cut(s) 105
GsuI CTGGAG 2 cut(s) 93, 282
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 3 cut(s) 347, 419, 1104
HapII CCGG 2 cut(s) 48, 906
HhaI GCGC 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 115
Hin1II CATG 8 cut(s) 227, 527, 577, 643, 805, 833, 856, 982
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HinfI GANTC 5 cut(s) 364, 467, 484, 768, 1097
HpaII CCGG 2 cut(s) 48, 906
HphI GGTGA 1 cut(s) 598
Hpy166II GTNNAC 2 cut(s) 443, 1117
Hpy188I TCNGA 3 cut(s) 201, 498, 773
Hpy8I GTNNAC 2 cut(s) 443, 1117
HpyAV CCTTC 6 cut(s) 148, 500, 542, 637, 871, 1069
HpyCH4III ACNGT 2 cut(s) 493, 1019
HpyCH4IV ACGT 3 cut(s) 405, 445, 592
HpyCH4V TGCA 2 cut(s) 410, 1079
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
HpyF3I CTNAG 3 cut(s) 618, 1052, 1086
HpySE526I ACGT 3 cut(s) 405, 445, 592
Hsp92I GRCGYC 1 cut(s) 115
Hsp92II CATG 8 cut(s) 227, 527, 577, 643, 805, 833, 856, 982
HspAI GCGC 1 cut(s) 115
KasI GGCGCC 1 cut(s) 114
Kpn2I TCCGGA 1 cut(s) 905
KpnI GGTACC 1 cut(s) 48
Kzo9I GATC 5 cut(s) 216, 258, 556, 804, 880
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 6 cut(s) 88, 291, 301, 917, 973, 1040
Lsp1109I GCAGC 1 cut(s) 805
LweI GCATC 7 cut(s) 70, 148, 274, 316, 556, 764, 1055
MaeI CTAG 3 cut(s) 438, 668, 686
MaeII ACGT 3 cut(s) 405, 445, 592
MaeIII GTNAC 3 cut(s) 35, 485, 596
MalI GATC 5 cut(s) 218, 260, 558, 806, 882
MbiI CCGCTC 1 cut(s) 622
MboI GATC 5 cut(s) 216, 258, 556, 804, 880
MboII GAAGA 6 cut(s) 199, 268, 314, 709, 730, 781
MfeI CAATTG 2 cut(s) 238, 1110
MflI RGATCY 2 cut(s) 258, 880
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 7 cut(s) 238, 369, 627, 650, 728, 930, 1110
Mly113I GGCGCC 1 cut(s) 115
MlyI GAGTC 4 cut(s) 358, 461, 493, 1106
MmeI TCCRAC 1 cut(s) 1087
MroI TCCGGA 1 cut(s) 905
MroXI GAANNNNTTC 1 cut(s) 1127
MseI TTAA 1 cut(s) 336
MslI CAYNNNNRTG 2 cut(s) 129, 131
MspI CCGG 2 cut(s) 48, 906
MspR9I CCNGG 3 cut(s) 416, 545, 927
MunI CAATTG 2 cut(s) 238, 1110
MvaI CCWGG 3 cut(s) 416, 545, 927
MwoI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
NarI GGCGCC 1 cut(s) 115
NdeII GATC 5 cut(s) 216, 258, 556, 804, 880
NlaIII CATG 8 cut(s) 227, 527, 577, 643, 805, 833, 856, 982
NlaIV GGNNCC 6 cut(s) 46, 116, 297, 913, 1036, 1105
NmuCI GTSAC 2 cut(s) 35, 485
NspI RCATGY 1 cut(s) 527
OliI CACNNNNGTG 2 cut(s) 129, 131
PagI TCATGA 2 cut(s) 573, 852
PciSI GCTCTTC 1 cut(s) 297
PdmI GAANNNNTTC 1 cut(s) 1127
PfeI GAWTC 1 cut(s) 768
PkrI GCNGC 1 cut(s) 820
Ple19I CGATCG 1 cut(s) 219
PleI GAGTC 4 cut(s) 358, 461, 492, 1105
PluTI GGCGCC 1 cut(s) 118
PpsI GAGTC 4 cut(s) 358, 461, 492, 1105
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 3 cut(s) 414, 543, 925
PspFI CCCAGC 1 cut(s) 101
PspGI CCWGG 3 cut(s) 414, 543, 925
PspN4I GGNNCC 6 cut(s) 46, 116, 297, 913, 1036, 1105
PspPI GGNCC 2 cut(s) 541, 1103
PstNI CAGNNNCTG 1 cut(s) 1023
PsuI RGATCY 2 cut(s) 258, 880
PvuI CGATCG 1 cut(s) 219
RsaI GTAC 3 cut(s) 46, 404, 448
RsaNI GTAC 3 cut(s) 45, 403, 447
RseI CAYNNNNRTG 2 cut(s) 129, 131
SacI GAGCTC 1 cut(s) 288
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 1 cut(s) 819
Sau3AI GATC 5 cut(s) 216, 258, 556, 804, 880
Sau96I GGNCC 2 cut(s) 541, 1103
SchI GAGTC 4 cut(s) 358, 461, 493, 1106
ScrFI CCNGG 3 cut(s) 416, 545, 927
SduI GDGCHC 1 cut(s) 288
SfaNI GCATC 7 cut(s) 70, 148, 274, 316, 556, 764, 1055
SfoI GGCGCC 1 cut(s) 116
SinI GGWCC 1 cut(s) 541
SmiMI CAYNNNNRTG 2 cut(s) 129, 131
Sse9I AATT 7 cut(s) 238, 369, 627, 650, 728, 930, 1110
SsiI CCGC 2 cut(s) 622, 1001
SspDI GGCGCC 1 cut(s) 114
SspMI CTAG 3 cut(s) 438, 668, 686
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 3 cut(s) 414, 543, 925
StyI CCWWGG 1 cut(s) 342
TaaI ACNGT 2 cut(s) 493, 1019
TaiI ACGT 3 cut(s) 408, 448, 595
TaqI TCGA 2 cut(s) 185, 219
TasI AATT 7 cut(s) 238, 369, 627, 650, 728, 930, 1110
TfiI GAWTC 1 cut(s) 768
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TscAI CASTG 1 cut(s) 1024
TseFI GTSAC 2 cut(s) 35, 485
TseI GCWGC 1 cut(s) 818
Tsp45I GTSAC 2 cut(s) 35, 485
TspDTI ATGAA 8 cut(s) 519, 590, 656, 690, 741, 846, 869, 995
TspGWI ACGGA 1 cut(s) 278
TspRI CASTG 1 cut(s) 1024
VpaK11BI GGWCC 1 cut(s) 541
XbaI TCTAGA 1 cut(s) 667
XceI RCATGY 1 cut(s) 527
XcmI CCANNNNNNNNNTGG 2 cut(s) 535, 808
XmnI GAANNNNTTC 1 cut(s) 1127
XspI CTAG 3 cut(s) 438, 668, 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.