MD12G1140800.v1.1

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000536
Physical Location & Seq
Forward (+)
21886901 .. 21889846
2946 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1140800.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGGTTAAGGCTGGATTTGCTGGAGATGATGCTCCAAGAGCCGTGTTCCCTAGCATTGTTGGCCGCCCACGACACACTGGTGTTATGGTTGGAATGGGTCAGAAAGATGCATATGTTGGGGATGAGGCTCAGTCCAAGCGTGGTATCTTAACCCTCAAGTACCCAATTGAGCACGGAATTGTGAGCAATTGGGATGACATGGAGAAGATTTGGCATCATACCTTCTATAATGAACTGCGTGTTGCACCCGAAGAGCACCCAATTCTCCTCACTGAAGCACCTCTCAACCCTAAAGCAAATCGTGAAAAGATGACCCAGATCATGTTTGAGACCTTCAATGCTCCAGCTATGTATGTAGCCATCCAGGCCGTGCTTTCCCTCTATGCCAGTGGTCGTACTACTGGTATCGTTCTTGACTCTGGAGATGGTGTCAGCCACACGGTCCCTATTTATGAAGGGTATGCTCTCCCACATGCCATCTTGAGGCTTGACCTTGCAGGTCGTGATCTGACTGATGCCTTGATGAAAATCTTGACTGAGCGTGGCTATTCATTCACCACCACAGCAGAGCGTGAAATTGTGAGGGACATGAAGGAAAAGTTGGCCTACATTGCTCTTGACTATGAACAGGAATTGGAAACTTCTAAAACCAGCTCTTCTGTTGAGAAGAGCTATGAGCTACCTGATGGTCAGGTGATCACTATTGGGGCAGAGCGTTTCCGATGCCCAGAAGTCCTCTTCCAACCATCCATGATCGGAATGGAGGCTGCTGGTATTCATGAGACCACTTACAATTCCATAATGAAATGCGATGTCGATATCAGGAAGGATTTGTATGGCAACATTGTTCTTAGTGGTGGTTCAACCATGTTCCCTGGAATTGCTGACCGAATGAGTAAGGAAATTACTGCATTGGCCCCAAGCAGCATGAAGATCAAGGTGGTTGCCCCACCAGAGAGAAAGTACAGTGTCTGGATCGGAGGCTCTATCTTAGCTTCCCTCAGTACCTTCCAGCAGATGTGGATTGCCAAAGCTGAGTACGATGAATCCGGTCCCTCAATCGTGCACAGGAAATGCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

361

Amino Acids

39.94

Weight (kDa)

5.67

Isoelectric Point (pI)

35.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 1 - 360 2.1e-141 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000536)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37620 AT2G37620 AT2G37620 AT2G37620 AT3G46520 AT3G46520 AT3G53750 AT3G53750 AT5G59370 AT5G59370
fragaria_vesca FvH4_3g35960 FvH4_6g13940 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_7g22410 FvH4_7g22410 FvH4_7g22410
malus_domestica MD03G1095300.v1.1 MD04G1127400.v1.1 MD11G1110300.v1.1 MD12G1056900.v1.1 MD12G1140800.v1.1 MD14G1056700.v1.1
prunus_persica Prupe.6G078800_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.7G077300_v2.0.a1 Prupe.7G077300_v2.0.a1
pyrus_communis pycom01g02640 pycom03g07780 pycom11g09110 pycom126g00040 pycom14g04650 pycom15g30330
rosa_chinensis RchiOBHm_Chr1g0367041 RchiOBHm_Chr3g0466761 RchiOBHm_Chr3g0479651 RchiOBHm_Chr5g0064311
rosa_laevigata RLG00000023550 RLG00000024555 RLG00000027304 RLG00000035715
rosa_multiflora Rmu_co8518979.1_g000001 Rmu_sc0002269.1_g000004 Rmu_sc0003467.1_g000022 Rmu_sc0003755.1_g000002 Rmu_sc0013440.1_g000001 Rmu_sc0013700.1_g000002 Rmu_sc0026659.1_g000001 Rmu_sc0037142.1_g000001
rosa_roxburghii Rroxscaffold_1G00016700 Rroxscaffold_4G00289680 Rroxscaffold_6G00402090
rosa_rugosa Rorug01G0334100 Rorug03G0083700 Rorug03G0083800 Rorug03G0177100 Rorug03G0177200 Rorug05G0364000 Rorug05G0364100
rosa_samantha Rh1AG340900 Rh1BG301400 Rh1CG317600 Rh1DG333800 Rh2AG276500 Rh3AG130800 Rh3AG227900 Rh3BG151800 Rh3BG261100 Rh3CG151700 Rh3DG152500 Rh3DG254400 Rh5AG421900 Rh5BG437600 Rh5CG460300 Rh5DG450700
rosa_wichuraiana Rw0G023870 Rw1G030280 Rw3G012360 Rw3G020390 Rw5G039710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 488
AciI CCGC 1 cut(s) 64
AclWI GGATC 1 cut(s) 983
AcoI YGGCCR 1 cut(s) 61
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 5 cut(s) 161, 397, 965, 1006, 1040
AfiI CCNNNNNNNGG 1 cut(s) 483
AgsI TTSAA 2 cut(s) 337, 864
AjnI CCWGG 2 cut(s) 363, 874
AjuI GAANNNNNNNTTGG 2 cut(s) 584, 616
AleI CACNNNNGTG 1 cut(s) 78
AluBI AGCT 6 cut(s) 347, 654, 672, 679, 995, 1034
AluI AGCT 6 cut(s) 347, 654, 672, 679, 995, 1034
Alw21I GWGCWC 3 cut(s) 174, 258, 1068
Alw26I GTCTC 2 cut(s) 323, 776
Alw44I GTGCAC 1 cut(s) 1064
AlwI GGATC 1 cut(s) 983
AlwNI CAGNNNCTG 1 cut(s) 972
AoxI GGCC 4 cut(s) 61, 366, 603, 915
ApaLI GTGCAC 1 cut(s) 1064
ApeKI GCWGC 2 cut(s) 767, 924
Asp700I GAANNNNTTC 1 cut(s) 1076
AspS9I GGNCC 3 cut(s) 442, 916, 1052
AsuHPI GGTGA 2 cut(s) 547, 706
AvaII GGWCC 2 cut(s) 442, 1052
BaeGI GKGCMC 1 cut(s) 1068
Bbv12I GWGCWC 3 cut(s) 174, 258, 1068
BbvI GCAGC 2 cut(s) 754, 936
BccI CCATC 5 cut(s) 368, 419, 485, 680, 754
BceAI ACGGC 2 cut(s) 26, 353
BciT130I CCWGG 2 cut(s) 365, 876
BclI TGATCA 1 cut(s) 696
BcoDI GTCTC 2 cut(s) 323, 776
BfaI CTAG 1 cut(s) 51
BfuAI ACCTGC 1 cut(s) 488
BglI GCCNNNNNGGC 1 cut(s) 365
BisI GCNGC 3 cut(s) 64, 768, 925
BlsI GCNGC 3 cut(s) 65, 769, 926
Bme1390I CCNGG 2 cut(s) 365, 876
Bme18I GGWCC 2 cut(s) 442, 1052
BmgT120I GGNCC 3 cut(s) 442, 916, 1052
BmiI GGNNCC 3 cut(s) 444, 918, 1054
BmrFI CCNGG 2 cut(s) 365, 876
BmsI GCATC 5 cut(s) 19, 97, 223, 505, 713
BpmI CTGGAG 3 cut(s) 42, 327, 441
BpuEI CTTGAG 2 cut(s) 140, 502
BsaBI GATNNNNATC 1 cut(s) 527
BsaI GGTCTC 2 cut(s) 323, 776
BsaJI CCNNGG 1 cut(s) 874
BsaWI WCCGGW 1 cut(s) 1049
BsaXI ACNNNNNCTCC 2 cut(s) 414, 444
Bsc4I CCNNNNNNNGG 1 cut(s) 483
Bse1I ACTGG 3 cut(s) 82, 387, 406
Bse3DI GCAATG 1 cut(s) 609
Bse8I GATNNNNATC 1 cut(s) 527
BseBI CCWGG 2 cut(s) 365, 876
BseDI CCNNGG 1 cut(s) 874
BseGI GGATG 4 cut(s) 127, 199, 360, 746
BseJI GATNNNNATC 1 cut(s) 527
BseLI CCNNNNNNNGG 1 cut(s) 483
BseMI GCAATG 1 cut(s) 609
BseMII CTCAG 4 cut(s) 143, 528, 1015, 1026
BseNI ACTGG 3 cut(s) 82, 387, 406
BseRI GAGGAG 1 cut(s) 257
BseSI GKGCMC 1 cut(s) 1068
BseXI GCAGC 2 cut(s) 754, 936
BshFI GGCC 4 cut(s) 63, 368, 605, 917
BsiHKAI GWGCWC 3 cut(s) 174, 258, 1068
BsiSI CCGG 1 cut(s) 1050
BslFI GGGAC 3 cut(s) 428, 599, 1038
BslI CCNNNNNNNGG 1 cut(s) 483
BsmAI GTCTC 2 cut(s) 323, 776
BsmFI GGGAC 3 cut(s) 428, 599, 1038
BsnI GGCC 4 cut(s) 63, 368, 605, 917
Bso31I GGTCTC 2 cut(s) 323, 776
Bsp1286I GDGCHC 3 cut(s) 174, 258, 1068
Bsp143I GATC 6 cut(s) 318, 505, 696, 753, 933, 975
BspACI CCGC 1 cut(s) 64
BspANI GGCC 4 cut(s) 63, 368, 605, 917
BspCNI CTCAG 4 cut(s) 142, 529, 1014, 1027
BspHI TCATGA 1 cut(s) 778
BspLI GGNNCC 3 cut(s) 444, 918, 1054
BspMI ACCTGC 1 cut(s) 488
BspPI GGATC 1 cut(s) 983
BspQI GCTCTTC 3 cut(s) 246, 661, 662
BspTNI GGTCTC 2 cut(s) 323, 776
BsrDI GCAATG 1 cut(s) 609
BsrI ACTGG 3 cut(s) 82, 387, 406
BssECI CCNNGG 1 cut(s) 874
BssMI GATC 6 cut(s) 318, 505, 696, 753, 933, 975
Bst2UI CCWGG 2 cut(s) 365, 876
Bst4CI ACNGT 2 cut(s) 442, 968
Bst6I CTCTTC 4 cut(s) 246, 661, 662, 743
BstDEI CTNAG 6 cut(s) 129, 537, 851, 991, 1001, 1035
BstF5I GGATG 4 cut(s) 127, 199, 360, 746
BstKTI GATC 6 cut(s) 321, 508, 699, 756, 936, 978
BstMAI GTCTC 2 cut(s) 323, 776
BstMBI GATC 6 cut(s) 318, 505, 696, 753, 933, 975
BstMWI GCNNNNNNNGC 5 cut(s) 17, 38, 60, 365, 611
BstNI CCWGG 2 cut(s) 365, 876
BstNSI RCATGY 1 cut(s) 476
BstSCI CCNGG 2 cut(s) 363, 874
BstSLI GKGCMC 1 cut(s) 1068
BstV1I GCAGC 2 cut(s) 754, 936
BsuRI GGCC 4 cut(s) 63, 368, 605, 917
BtgZI GCGATG 1 cut(s) 825
BtsCI GGATG 4 cut(s) 127, 199, 360, 746
BtsIMutI CAGTG 4 cut(s) 75, 270, 394, 973
BveI ACCTGC 1 cut(s) 488
CaiI CAGNNNCTG 1 cut(s) 972
CciI TCATGA 1 cut(s) 778
Cfr13I GGNCC 3 cut(s) 442, 916, 1052
Csp6I GTAC 5 cut(s) 160, 396, 964, 1005, 1039
CviAII CATG 8 cut(s) 199, 322, 473, 589, 751, 779, 868, 928
CviQI GTAC 5 cut(s) 160, 396, 964, 1005, 1039
DdeI CTNAG 6 cut(s) 129, 537, 851, 991, 1001, 1035
DpnI GATC 6 cut(s) 320, 507, 698, 755, 935, 977
DpnII GATC 6 cut(s) 318, 505, 696, 753, 933, 975
EaeI YGGCCR 1 cut(s) 61
Eam1104I CTCTTC 4 cut(s) 246, 661, 662, 743
EarI CTCTTC 4 cut(s) 246, 661, 662, 743
Eco31I GGTCTC 2 cut(s) 323, 776
Eco32I GATATC 1 cut(s) 820
Eco47I GGWCC 2 cut(s) 442, 1052
Eco57I CTGAAG 1 cut(s) 294
EcoRII CCWGG 2 cut(s) 363, 874
EcoRV GATATC 1 cut(s) 820
EcoT22I ATGCAT 1 cut(s) 112
FaeI CATG 8 cut(s) 202, 325, 476, 592, 754, 782, 871, 931
FaqI GGGAC 3 cut(s) 428, 599, 1038
FatI CATG 8 cut(s) 198, 321, 472, 588, 750, 778, 867, 927
FauNDI CATATG 1 cut(s) 112
FbaI TGATCA 1 cut(s) 696
Fnu4HI GCNGC 3 cut(s) 64, 768, 925
FokI GGATG 4 cut(s) 134, 206, 347, 733
Fsp4HI GCNGC 3 cut(s) 64, 768, 925
FspBI CTAG 1 cut(s) 51
GluI GCNGC 3 cut(s) 64, 768, 925
GsuI CTGGAG 3 cut(s) 42, 327, 441
HaeIII GGCC 4 cut(s) 63, 368, 605, 917
HapII CCGG 1 cut(s) 1050
Hin1II CATG 8 cut(s) 202, 325, 476, 592, 754, 782, 871, 931
HinfI GANTC 2 cut(s) 416, 1046
HpaII CCGG 1 cut(s) 1050
HphI GGTGA 2 cut(s) 547, 706
Hpy166II GTNNAC 1 cut(s) 1066
Hpy188I TCNGA 6 cut(s) 102, 510, 722, 758, 980, 1082
Hpy8I GTNNAC 1 cut(s) 1066
HpyAV CCTTC 6 cut(s) 232, 343, 449, 586, 820, 1018
HpyCH4III ACNGT 2 cut(s) 442, 968
HpyCH4V TGCA 5 cut(s) 110, 245, 497, 911, 1066
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 38, 60, 365, 611
HpyF3I CTNAG 6 cut(s) 129, 537, 851, 991, 1001, 1035
Hsp92II CATG 8 cut(s) 202, 325, 476, 592, 754, 782, 871, 931
Ksp22I TGATCA 1 cut(s) 696
Kzo9I GATC 6 cut(s) 318, 505, 696, 753, 933, 975
LguI GCTCTTC 3 cut(s) 246, 661, 662
LmnI GCTCC 2 cut(s) 37, 346
Lsp1109I GCAGC 2 cut(s) 754, 936
LweI GCATC 5 cut(s) 19, 97, 223, 505, 713
MaeI CTAG 1 cut(s) 51
MalI GATC 6 cut(s) 320, 507, 698, 755, 935, 977
MboI GATC 6 cut(s) 318, 505, 696, 753, 933, 975
MboII GAAGA 6 cut(s) 217, 263, 648, 679, 730, 943
MfeI CAATTG 2 cut(s) 165, 187
MhlI GDGCHC 3 cut(s) 174, 258, 1068
MluCI AATT 9 cut(s) 165, 177, 187, 261, 576, 632, 793, 879, 903
MlyI GAGTC 1 cut(s) 410
MmeI TCCRAC 2 cut(s) 70, 766
Mph1103I ATGCAT 1 cut(s) 112
MroXI GAANNNNTTC 1 cut(s) 1076
MseI TTAA 2 cut(s) 6, 149
MslI CAYNNNNRTG 1 cut(s) 78
MspI CCGG 1 cut(s) 1050
MspR9I CCNGG 2 cut(s) 365, 876
MunI CAATTG 2 cut(s) 165, 187
MvaI CCWGG 2 cut(s) 365, 876
MwoI GCNNNNNNNGC 5 cut(s) 17, 38, 60, 365, 611
NdeI CATATG 1 cut(s) 112
NdeII GATC 6 cut(s) 318, 505, 696, 753, 933, 975
NlaIII CATG 8 cut(s) 202, 325, 476, 592, 754, 782, 871, 931
NlaIV GGNNCC 3 cut(s) 444, 918, 1054
NsiI ATGCAT 1 cut(s) 112
NspI RCATGY 1 cut(s) 476
OliI CACNNNNGTG 1 cut(s) 78
PagI TCATGA 1 cut(s) 778
PciSI GCTCTTC 3 cut(s) 246, 661, 662
PdmI GAANNNNTTC 1 cut(s) 1076
PfeI GAWTC 1 cut(s) 1046
PkrI GCNGC 3 cut(s) 65, 769, 926
PleI GAGTC 1 cut(s) 410
PpsI GAGTC 1 cut(s) 410
Psp6I CCWGG 2 cut(s) 363, 874
PspGI CCWGG 2 cut(s) 363, 874
PspN4I GGNNCC 3 cut(s) 444, 918, 1054
PspPI GGNCC 3 cut(s) 442, 916, 1052
PstNI CAGNNNCTG 1 cut(s) 972
RsaI GTAC 5 cut(s) 161, 397, 965, 1006, 1040
RsaNI GTAC 5 cut(s) 160, 396, 964, 1005, 1039
RseI CAYNNNNRTG 1 cut(s) 78
SapI GCTCTTC 3 cut(s) 246, 661, 662
SaqAI TTAA 2 cut(s) 6, 149
SatI GCNGC 3 cut(s) 64, 768, 925
Sau3AI GATC 6 cut(s) 318, 505, 696, 753, 933, 975
Sau96I GGNCC 3 cut(s) 442, 916, 1052
SchI GAGTC 1 cut(s) 410
ScrFI CCNGG 2 cut(s) 365, 876
SduI GDGCHC 3 cut(s) 174, 258, 1068
SfaNI GCATC 5 cut(s) 19, 97, 223, 505, 713
SinI GGWCC 2 cut(s) 442, 1052
SmiMI CAYNNNNRTG 1 cut(s) 78
SmlI CTYRAG 2 cut(s) 155, 481
SmoI CTYRAG 2 cut(s) 155, 481
Sse9I AATT 9 cut(s) 165, 177, 187, 261, 576, 632, 793, 879, 903
SsiI CCGC 1 cut(s) 64
SspMI CTAG 1 cut(s) 51
StyD4I CCNGG 2 cut(s) 363, 874
TaaI ACNGT 2 cut(s) 442, 968
TaqI TCGA 1 cut(s) 816
TaqII GACCGA 1 cut(s) 903
TasI AATT 9 cut(s) 165, 177, 187, 261, 576, 632, 793, 879, 903
TatI WGTACW 1 cut(s) 963
TauI GCSGC 1 cut(s) 66
TfiI GAWTC 1 cut(s) 1046
Tru1I TTAA 2 cut(s) 6, 149
Tru9I TTAA 2 cut(s) 6, 149
TscAI CASTG 4 cut(s) 82, 277, 394, 973
TseI GCWGC 2 cut(s) 767, 924
TspGWI ACGGA 1 cut(s) 189
TspRI CASTG 4 cut(s) 82, 277, 394, 973
VneI GTGCAC 1 cut(s) 1064
VpaK11BI GGWCC 2 cut(s) 442, 1052
XceI RCATGY 1 cut(s) 476
XcmI CCANNNNNNNNNTGG 1 cut(s) 757
XmnI GAANNNNTTC 1 cut(s) 1076
XspI CTAG 1 cut(s) 51
Zsp2I ATGCAT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.