Rw1G030280

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000536
Physical Location & Seq
Forward (+)
58241290 .. 58243238
1949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G030280.1

Sequence Viewer

Length: 1134 bp
ATGGCAGAAAGTGAGGACATTCAGCCGCTCGTGTGTGATAATGGAACCGGAATGGTCAAGGCCGGGTTTGCTGGAGATGATGCTCCGCGAGCTGTTTTTCCTAGCATTGTAGGGCGCCCTCGTCACACTGGTGTCATGGTTGGCATGGGCCAGAAAGATGCTTATGTTGGTGACGAGGCTCAATCCAAGCGAGGTATCTTGACTTTGAAATATCCAATTGAGCATGGTATTGTCAGCAACTGGGATGACATGGAGAAGATTTGGCATCACACCTTCTACAATGAGCTTCGTGTTGCCCCAGAAGAGCACCCAGTTCTGCTAACTGAGGCCCCCCTGAATCCCAAGGCTAATCGTGAGAAGATGACCCAGATAATGTTCGAGACCTTTAACACTCCTGCTATGTATGTGGCCATTCAAGCTGTTCTTTCCCTGTATGCAAGTGGGCGTACAACTGGTATTGTGCTTGACTCTGGTGATGGTGTCAGCCATACAGTCCCCATATACGAGGGATATGCCCTCCCTCATGCCATCCTTCGTCTTGACCTTGCTGGTCGTGATCTCACTGATAGCTTGATGAAAATTCTTACCGAGCGTGGATATTCTTTCACTACCACTGCTGAGCGTGAAATTGTTAGGGACATGAAGGAAAAACTTGCTTACATTGCCCTTGACTATGAGCAGGAGCTGGAGACATCCAAAACCAGCTCCTCAGTTGAGAAGAGCTATGAGTTACCAGATGGGCAGGTCATTACCATTGGTGCAGAGCGTTTCAGATGCCCAGAAGTCCTTTTCCAACCCTCAATGATCGGAATGGAAGCAGCTGGCATTCATGAGACCACTTACAACTCAATCATGAAGTGTGATGTTGATATCAGGAAAGACCTTTACGGAAATATTGTACTTTCTGGTGGTTCAACTATGTTTCCAGGCATTGCTGATAGGATGAGCAAGGAAATTTCTGCTCTCGCCCCAAGCAGCATGAAGATCAAGGTGGTGGCACCACCTGAGAGGAAGTACAGTGTCTGGATTGGAGGCTCCATCTTGGCTTCGCTCAGTACCTTCCAGCAGATGTGGATTGCAAAAGCGGAGTATGATGAGTCTGGGCCGTCCATAGTTCATAGGAAATGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

377

Amino Acids

41.72

Weight (kDa)

5.31

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 5 - 377 2.6e-146 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000536)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37620 AT2G37620 AT2G37620 AT2G37620 AT3G46520 AT3G46520 AT3G53750 AT3G53750 AT5G59370 AT5G59370
fragaria_vesca FvH4_3g35960 FvH4_6g13940 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_7g22410 FvH4_7g22410 FvH4_7g22410
malus_domestica MD03G1095300.v1.1 MD04G1127400.v1.1 MD11G1110300.v1.1 MD12G1056900.v1.1 MD12G1140800.v1.1 MD14G1056700.v1.1
prunus_persica Prupe.6G078800_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.7G077300_v2.0.a1 Prupe.7G077300_v2.0.a1
pyrus_communis pycom01g02640 pycom03g07780 pycom11g09110 pycom126g00040 pycom14g04650 pycom15g30330
rosa_chinensis RchiOBHm_Chr1g0367041 RchiOBHm_Chr3g0466761 RchiOBHm_Chr3g0479651 RchiOBHm_Chr5g0064311
rosa_laevigata RLG00000023550 RLG00000024555 RLG00000027304 RLG00000035715
rosa_multiflora Rmu_co8518979.1_g000001 Rmu_sc0002269.1_g000004 Rmu_sc0003467.1_g000022 Rmu_sc0003755.1_g000002 Rmu_sc0013440.1_g000001 Rmu_sc0013700.1_g000002 Rmu_sc0026659.1_g000001 Rmu_sc0037142.1_g000001
rosa_roxburghii Rroxscaffold_1G00016700 Rroxscaffold_4G00289680 Rroxscaffold_6G00402090
rosa_rugosa Rorug01G0334100 Rorug03G0083700 Rorug03G0083800 Rorug03G0177100 Rorug03G0177200 Rorug05G0364000 Rorug05G0364100
rosa_samantha Rh1AG340900 Rh1BG301400 Rh1CG317600 Rh1DG333800 Rh2AG276500 Rh3AG130800 Rh3AG227900 Rh3BG151800 Rh3BG261100 Rh3CG151700 Rh3DG152500 Rh3DG254400 Rh5AG421900 Rh5BG437600 Rh5CG460300 Rh5DG450700
rosa_wichuraiana Rw0G023870 Rw1G030280 Rw3G012360 Rw3G020390 Rw5G039710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 733
AccB1I GGYRCC 2 cut(s) 114, 997
AccBSI CCGCTC 1 cut(s) 28
AccII CGCG 1 cut(s) 88
AciI CCGC 3 cut(s) 26, 86, 1085
AcoI YGGCCR 1 cut(s) 408
AcsI RAATTY 2 cut(s) 579, 954
AcyI GRCGYC 1 cut(s) 115
AfaI GTAC 4 cut(s) 448, 900, 1016, 1057
AgsI TTSAA 3 cut(s) 208, 416, 915
AjnI CCWGG 1 cut(s) 925
AleI CACNNNNGTG 1 cut(s) 129
AluBI AGCT 8 cut(s) 92, 286, 419, 570, 685, 705, 723, 821
AluI AGCT 8 cut(s) 92, 286, 419, 570, 685, 705, 723, 821
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 3 cut(s) 374, 683, 827
AlwNI CAGNNNCTG 3 cut(s) 240, 685, 1023
AoxI GGCC 5 cut(s) 60, 148, 327, 408, 1103
ApeKI GCWGC 2 cut(s) 818, 975
ApoI RAATTY 2 cut(s) 579, 954
Asp700I GAANNNNTTC 1 cut(s) 1127
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 3 cut(s) 148, 328, 1103
AsuC2I CCSGG 1 cut(s) 64
AsuHPI GGTGA 2 cut(s) 182, 485
BalI TGGCCA 1 cut(s) 410
BanI GGYRCC 2 cut(s) 114, 997
BauI CACGAG 1 cut(s) 29
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 2 cut(s) 830, 987
BccI CCATC 4 cut(s) 470, 536, 731, 1046
BceAI ACGGC 1 cut(s) 1090
BcgI CGANNNNNNTGC 2 cut(s) 494, 528
BciT130I CCWGG 1 cut(s) 927
BcnI CCSGG 1 cut(s) 64
BcoDI GTCTC 3 cut(s) 374, 683, 827
BfaI CTAG 1 cut(s) 102
BfoI RGCGCY 1 cut(s) 118
BfuAI ACCTGC 1 cut(s) 733
BisI GCNGC 3 cut(s) 26, 819, 976
BlpI GCTNAGC 1 cut(s) 618
BlsI GCNGC 3 cut(s) 27, 820, 977
Bme1390I CCNGG 2 cut(s) 64, 927
BmgT120I GGNCC 3 cut(s) 148, 328, 1103
BmiI GGNNCC 5 cut(s) 46, 116, 330, 999, 1036
BmrFI CCNGG 2 cut(s) 64, 927
BmrI ACTGGG 2 cut(s) 250, 305
BmsI GCATC 4 cut(s) 70, 148, 274, 764
BmuI ACTGGG 2 cut(s) 250, 305
BpmI CTGGAG 2 cut(s) 93, 707
Bpu1102I GCTNAGC 1 cut(s) 618
BpuMI CCSGG 1 cut(s) 64
BsaHI GRCGYC 1 cut(s) 115
BsaI GGTCTC 2 cut(s) 374, 827
BsaJI CCNNGG 1 cut(s) 342
BsaWI WCCGGW 1 cut(s) 47
Bse1I ACTGG 4 cut(s) 133, 245, 311, 457
Bse3DI GCAATG 2 cut(s) 660, 930
BseBI CCWGG 1 cut(s) 927
BseDI CCNNGG 1 cut(s) 342
BseGI GGATG 4 cut(s) 250, 528, 692, 948
BseMI GCAATG 2 cut(s) 660, 930
BseMII CTCAG 5 cut(s) 315, 609, 723, 996, 1066
BseNI ACTGG 4 cut(s) 133, 245, 311, 457
BseRI GAGGAG 1 cut(s) 697
BseXI GCAGC 2 cut(s) 830, 987
BsgI GTGCAG 1 cut(s) 780
Bsh1236I CGCG 1 cut(s) 88
BshFI GGCC 5 cut(s) 62, 150, 329, 410, 1105
BshNI GGYRCC 2 cut(s) 114, 997
BsiHKAI GWGCWC 1 cut(s) 309
BsiSI CCGG 2 cut(s) 48, 63
BslFI GGGAC 2 cut(s) 479, 650
BsmAI GTCTC 3 cut(s) 374, 683, 827
BsmFI GGGAC 2 cut(s) 479, 650
BsmI GAATGC 1 cut(s) 825
BsnI GGCC 5 cut(s) 62, 150, 329, 410, 1105
Bso31I GGTCTC 2 cut(s) 374, 827
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 3 cut(s) 556, 804, 984
Bsp1720I GCTNAGC 1 cut(s) 618
BspACI CCGC 3 cut(s) 26, 86, 1085
BspANI GGCC 5 cut(s) 62, 150, 329, 410, 1105
BspCNI CTCAG 5 cut(s) 316, 610, 722, 997, 1065
BspFNI CGCG 1 cut(s) 88
BspHI TCATGA 2 cut(s) 829, 852
BspLI GGNNCC 5 cut(s) 46, 116, 330, 999, 1036
BspMI ACCTGC 1 cut(s) 733
BspQI GCTCTTC 2 cut(s) 297, 713
BspT107I GGYRCC 2 cut(s) 114, 997
BspTNI GGTCTC 2 cut(s) 374, 827
BsrBI CCGCTC 1 cut(s) 28
BsrDI GCAATG 2 cut(s) 660, 930
BsrI ACTGG 4 cut(s) 133, 245, 311, 457
BssECI CCNNGG 1 cut(s) 342
BssMI GATC 3 cut(s) 556, 804, 984
BssNI GRCGYC 1 cut(s) 115
BssSI CACGAG 1 cut(s) 29
BssT1I CCWWGG 1 cut(s) 342
Bst2BI CACGAG 1 cut(s) 29
Bst2UI CCWGG 1 cut(s) 927
Bst4CI ACNGT 2 cut(s) 493, 1019
Bst6I CTCTTC 2 cut(s) 297, 713
BstACI GRCGYC 1 cut(s) 115
BstC8I GCNNGC 2 cut(s) 90, 823
BstDEI CTNAG 5 cut(s) 324, 618, 709, 1005, 1052
BstF5I GGATG 4 cut(s) 250, 528, 692, 948
BstFNI CGCG 1 cut(s) 88
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 3 cut(s) 559, 807, 987
BstMAI GTCTC 3 cut(s) 374, 683, 827
BstMBI GATC 3 cut(s) 556, 804, 984
BstMWI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
BstNI CCWGG 1 cut(s) 927
BstSCI CCNGG 2 cut(s) 62, 925
BstUI CGCG 1 cut(s) 88
BstV1I GCAGC 2 cut(s) 830, 987
BsuRI GGCC 5 cut(s) 62, 150, 329, 410, 1105
BtsCI GGATG 4 cut(s) 250, 528, 692, 948
BtsI GCAGTG 1 cut(s) 612
BtsIMutI CAGTG 4 cut(s) 126, 561, 612, 1024
BveI ACCTGC 1 cut(s) 733
Cac8I GCNNGC 2 cut(s) 90, 823
CaiI CAGNNNCTG 3 cut(s) 240, 685, 1023
CciI TCATGA 2 cut(s) 829, 852
CfoI GCGC 1 cut(s) 117
Cfr13I GGNCC 3 cut(s) 148, 328, 1103
Csp6I GTAC 4 cut(s) 447, 899, 1015, 1056
CviAII CATG 9 cut(s) 136, 145, 224, 250, 524, 640, 830, 853, 979
CviQI GTAC 4 cut(s) 447, 899, 1015, 1056
DdeI CTNAG 5 cut(s) 324, 618, 709, 1005, 1052
DinI GGCGCC 1 cut(s) 116
DpnI GATC 3 cut(s) 558, 806, 986
DpnII GATC 3 cut(s) 556, 804, 984
EaeI YGGCCR 1 cut(s) 408
Eam1104I CTCTTC 2 cut(s) 297, 713
EarI CTCTTC 2 cut(s) 297, 713
Eco130I CCWWGG 1 cut(s) 342
Eco31I GGTCTC 2 cut(s) 374, 827
Eco32I GATATC 1 cut(s) 871
EcoO109I RGGNCCY 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 925
EcoRV GATATC 1 cut(s) 871
EcoT14I CCWWGG 1 cut(s) 342
EgeI GGCGCC 1 cut(s) 116
EheI GGCGCC 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 342
FaeI CATG 9 cut(s) 139, 148, 227, 253, 527, 643, 833, 856, 982
FalI AAGNNNNNCTT 2 cut(s) 408, 440
FaqI GGGAC 2 cut(s) 479, 650
FatI CATG 9 cut(s) 135, 144, 223, 249, 523, 639, 829, 852, 978
Fnu4HI GCNGC 3 cut(s) 26, 819, 976
FokI GGATG 4 cut(s) 257, 515, 679, 955
Fsp4HI GCNGC 3 cut(s) 26, 819, 976
FspBI CTAG 1 cut(s) 102
GlaI GCGC 1 cut(s) 116
GluI GCNGC 3 cut(s) 26, 819, 976
GsuI CTGGAG 2 cut(s) 93, 707
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 5 cut(s) 62, 150, 329, 410, 1105
HapII CCGG 2 cut(s) 48, 63
HhaI GCGC 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 115
Hin1II CATG 9 cut(s) 139, 148, 227, 253, 527, 643, 833, 856, 982
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HinfI GANTC 3 cut(s) 337, 467, 1097
HpaII CCGG 2 cut(s) 48, 63
HphI GGTGA 2 cut(s) 182, 485
Hpy188I TCNGA 2 cut(s) 773, 809
Hpy188III TCNNGA 9 cut(s) 199, 353, 379, 539, 554, 830, 853, 874, 1024
HpyAV CCTTC 4 cut(s) 283, 542, 637, 1069
HpyCH4III ACNGT 2 cut(s) 493, 1019
HpyCH4V TGCA 3 cut(s) 437, 761, 1079
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
HpyF3I CTNAG 5 cut(s) 324, 618, 709, 1005, 1052
Hsp92I GRCGYC 1 cut(s) 115
Hsp92II CATG 9 cut(s) 139, 148, 227, 253, 527, 643, 833, 856, 982
HspAI GCGC 1 cut(s) 115
KasI GGCGCC 1 cut(s) 114
Kzo9I GATC 3 cut(s) 556, 804, 984
LguI GCTCTTC 2 cut(s) 297, 713
LmnI GCTCC 4 cut(s) 88, 682, 710, 1040
Lsp1109I GCAGC 2 cut(s) 830, 987
LweI GCATC 4 cut(s) 70, 148, 274, 764
MaeI CTAG 1 cut(s) 102
MaeIII GTNAC 3 cut(s) 122, 170, 729
MalI GATC 3 cut(s) 558, 806, 986
MbiI CCGCTC 1 cut(s) 28
MboI GATC 3 cut(s) 556, 804, 984
MboII GAAGA 5 cut(s) 268, 314, 370, 730, 994
MfeI CAATTG 1 cut(s) 216
MhlI GDGCHC 1 cut(s) 309
MlsI TGGCCA 1 cut(s) 410
MluCI AATT 4 cut(s) 216, 579, 627, 954
MluNI TGGCCA 1 cut(s) 410
Mly113I GGCGCC 1 cut(s) 115
MlyI GAGTC 2 cut(s) 461, 1106
MmeI TCCRAC 1 cut(s) 817
Mox20I TGGCCA 1 cut(s) 410
MroXI GAANNNNTTC 1 cut(s) 1127
MscI TGGCCA 1 cut(s) 410
MseI TTAA 1 cut(s) 387
MslI CAYNNNNRTG 1 cut(s) 129
Msp20I TGGCCA 1 cut(s) 410
MspA1I CMGCKG 1 cut(s) 821
MspI CCGG 2 cut(s) 48, 63
MspR9I CCNGG 2 cut(s) 64, 927
MunI CAATTG 1 cut(s) 216
Mva1269I GAATGC 1 cut(s) 825
MvaI CCWGG 1 cut(s) 927
MvnI CGCG 1 cut(s) 88
MwoI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
NarI GGCGCC 1 cut(s) 115
NciI CCSGG 1 cut(s) 64
NdeII GATC 3 cut(s) 556, 804, 984
NlaIII CATG 9 cut(s) 139, 148, 227, 253, 527, 643, 833, 856, 982
NlaIV GGNNCC 5 cut(s) 46, 116, 330, 999, 1036
NmuCI GTSAC 2 cut(s) 122, 170
OliI CACNNNNGTG 1 cut(s) 129
PagI TCATGA 2 cut(s) 829, 852
PciSI GCTCTTC 2 cut(s) 297, 713
PctI GAATGC 1 cut(s) 825
PdmI GAANNNNTTC 1 cut(s) 1127
PfeI GAWTC 1 cut(s) 337
PkrI GCNGC 3 cut(s) 27, 820, 977
PleI GAGTC 2 cut(s) 461, 1105
PluTI GGCGCC 1 cut(s) 118
PpsI GAGTC 2 cut(s) 461, 1105
Psp6I CCWGG 1 cut(s) 925
PspGI CCWGG 1 cut(s) 925
PspN4I GGNNCC 5 cut(s) 46, 116, 330, 999, 1036
PspPI GGNCC 3 cut(s) 148, 328, 1103
PstNI CAGNNNCTG 3 cut(s) 240, 685, 1023
PvuII CAGCTG 1 cut(s) 821
RsaI GTAC 4 cut(s) 448, 900, 1016, 1057
RsaNI GTAC 4 cut(s) 447, 899, 1015, 1056
RseI CAYNNNNRTG 1 cut(s) 129
SapI GCTCTTC 2 cut(s) 297, 713
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 3 cut(s) 26, 819, 976
Sau3AI GATC 3 cut(s) 556, 804, 984
Sau96I GGNCC 3 cut(s) 148, 328, 1103
SchI GAGTC 2 cut(s) 461, 1106
ScrFI CCNGG 2 cut(s) 64, 927
SduI GDGCHC 1 cut(s) 309
SfaNI GCATC 4 cut(s) 70, 148, 274, 764
SfoI GGCGCC 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 129
Sse9I AATT 4 cut(s) 216, 579, 627, 954
SsiI CCGC 3 cut(s) 26, 86, 1085
SspDI GGCGCC 1 cut(s) 114
SspI AATATT 1 cut(s) 895
SspMI CTAG 1 cut(s) 102
StyD4I CCNGG 2 cut(s) 62, 925
StyI CCWWGG 1 cut(s) 342
TaaI ACNGT 2 cut(s) 493, 1019
TaqI TCGA 1 cut(s) 378
TasI AATT 4 cut(s) 216, 579, 627, 954
TatI WGTACW 2 cut(s) 898, 1014
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 1 cut(s) 337
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 4 cut(s) 133, 568, 619, 1024
TseFI GTSAC 2 cut(s) 122, 170
TseI GCWGC 2 cut(s) 818, 975
Tsp45I GTSAC 2 cut(s) 122, 170
TspDTI ATGAA 6 cut(s) 590, 656, 818, 869, 995, 1106
TspGWI ACGGA 1 cut(s) 903
TspRI CASTG 4 cut(s) 133, 568, 619, 1024
XapI RAATTY 2 cut(s) 579, 954
XmnI GAANNNNTTC 1 cut(s) 1127
XspI CTAG 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.