Rh3DG254400

Actin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000536
Physical Location & Seq
Forward (+)
25191979 .. 25196369
4391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG254400.1

Sequence Viewer

Length: 828 bp
ATGCTTTGCTGTTCAGGTATTGTTCTTGACTCTGGGGATGGTGTGAGTCACACAGTTCCGATATATGAAGGGTATGCCCTCCCACATGCCATCCTTCGTTTGGACCTGGCAGGTCGTGATCTTACAGATGCCCTCATGAAAATCTTGACTGAACGTGGTTACTCTTTCACCACTACTGCTGAGCGGGAAATTGTTAGAGACATGAAGGAGAAATTAGCCTATATTGCTCTAGACTATGAACAAGAACTAGAGACAGCAAAGACCAGCTCATCTGTTGAGAAGAGTTATGAATTACCTGATGGGCAGGTTATCACGATTGGTGCTGAAAGATTCCGATGCCCTGAAGTCCTCTTCCAGCCATCCATGATCGGGATGGAAGCTGCTGGTATCCATGAAACCACATATAACTCTATCATGAAGTGTGATGTTGATATCAGGAAGGATCTCTATGGCAACATTGTTCTTTCCGGAGGCTCCACTATGTTCCCTGGAATTGCTGATAGGATGAGCAAAGAGATTACAGCATTAGCTCCAAGTAGCATGAAAATCAAGGTCGTAGCACCGCCTGAGAGGAAATACAGTGTCTGGATAGGAGGCTCCATTTTGGCATCCCTCAGCACCTTCCAACAGGTACTGTGCTGTGTGCAAATGCACATCTGCTTAGGCACTCTACTAATGCATTTCTCGTTCAACTTCCATCCTTCCATGTCTTTTGTACATCTATATGTTAAAAGTCACCGTAAGATGACTATAACGCCTAGTTTGCAGATACATGTTACACATATGGCACACCATTGCACAATTGACTTGAAATCCCATGAGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

275

Amino Acids

30.67

Weight (kDa)

6.22

Isoelectric Point (pI)

38.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 5 - 211 3.1e-70 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000536)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37620 AT2G37620 AT2G37620 AT2G37620 AT3G46520 AT3G46520 AT3G53750 AT3G53750 AT5G59370 AT5G59370
fragaria_vesca FvH4_3g35960 FvH4_6g13940 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_7g22410 FvH4_7g22410 FvH4_7g22410
malus_domestica MD03G1095300.v1.1 MD04G1127400.v1.1 MD11G1110300.v1.1 MD12G1056900.v1.1 MD12G1140800.v1.1 MD14G1056700.v1.1
prunus_persica Prupe.6G078800_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.7G077300_v2.0.a1 Prupe.7G077300_v2.0.a1
pyrus_communis pycom01g02640 pycom03g07780 pycom11g09110 pycom126g00040 pycom14g04650 pycom15g30330
rosa_chinensis RchiOBHm_Chr1g0367041 RchiOBHm_Chr3g0466761 RchiOBHm_Chr3g0479651 RchiOBHm_Chr5g0064311
rosa_laevigata RLG00000023550 RLG00000024555 RLG00000027304 RLG00000035715
rosa_multiflora Rmu_co8518979.1_g000001 Rmu_sc0002269.1_g000004 Rmu_sc0003467.1_g000022 Rmu_sc0003755.1_g000002 Rmu_sc0013440.1_g000001 Rmu_sc0013700.1_g000002 Rmu_sc0026659.1_g000001 Rmu_sc0037142.1_g000001
rosa_roxburghii Rroxscaffold_1G00016700 Rroxscaffold_4G00289680 Rroxscaffold_6G00402090
rosa_rugosa Rorug01G0334100 Rorug03G0083700 Rorug03G0083800 Rorug03G0177100 Rorug03G0177200 Rorug05G0364000 Rorug05G0364100
rosa_samantha Rh1AG340900 Rh1BG301400 Rh1CG317600 Rh1DG333800 Rh2AG276500 Rh3AG130800 Rh3AG227900 Rh3BG151800 Rh3BG261100 Rh3CG151700 Rh3DG152500 Rh3DG254400 Rh5AG421900 Rh5BG437600 Rh5CG460300 Rh5DG450700
rosa_wichuraiana Rw0G023870 Rw1G030280 Rw3G012360 Rw3G020390 Rw5G039710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 101, 295
AccBSI CCGCTC 1 cut(s) 184
AccIII TCCGGA 1 cut(s) 467
AciI CCGC 2 cut(s) 184, 563
AclWI GGATC 1 cut(s) 450
AcuI CTGAAG 1 cut(s) 363
AfaI GTAC 2 cut(s) 633, 717
AfiI CCNNNNNNNGG 2 cut(s) 100, 369
AflIII ACRYGT 1 cut(s) 772
AgsI TTSAA 2 cut(s) 691, 811
AjnI CCWGG 2 cut(s) 105, 487
AluBI AGCT 3 cut(s) 267, 380, 530
AluI AGCT 3 cut(s) 267, 380, 530
Alw26I GTCTC 2 cut(s) 192, 245
AlwI GGATC 1 cut(s) 450
AlwNI CAGNNNCTG 2 cut(s) 585, 634
Aor13HI TCCGGA 1 cut(s) 467
ApeKI GCWGC 1 cut(s) 380
AspS9I GGNCC 1 cut(s) 103
AsuHPI GGTGA 2 cut(s) 160, 728
AvaII GGWCC 1 cut(s) 103
BbvCI CCTCAGC 1 cut(s) 614
BbvI GCAGC 1 cut(s) 367
BccI CCATC 6 cut(s) 32, 98, 293, 367, 367, 705
BciT130I CCWGG 2 cut(s) 107, 489
BciVI GTATCC 1 cut(s) 398
BcoDI GTCTC 2 cut(s) 192, 245
BfaI CTAG 3 cut(s) 230, 248, 759
BfuAI ACCTGC 2 cut(s) 101, 295
BfuI GTATCC 1 cut(s) 398
BisI GCNGC 1 cut(s) 381
BlpI GCTNAGC 1 cut(s) 180
BlsI GCNGC 1 cut(s) 382
Bme1390I CCNGG 2 cut(s) 107, 489
Bme18I GGWCC 1 cut(s) 103
BmgT120I GGNCC 1 cut(s) 103
BmiI GGNNCC 2 cut(s) 475, 598
BmrFI CCNGG 2 cut(s) 107, 489
BmsI GCATC 3 cut(s) 118, 326, 617
Bpu10I CCTNAGC 2 cut(s) 614, 661
Bpu1102I GCTNAGC 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 487
BsaWI WCCGGW 1 cut(s) 467
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 369
Bse3DI GCAATG 1 cut(s) 793
BseAI TCCGGA 1 cut(s) 467
BseBI CCWGG 2 cut(s) 107, 489
BseDI CCNNGG 1 cut(s) 487
BseGI GGATG 7 cut(s) 43, 90, 359, 378, 510, 608, 697
BseLI CCNNNNNNNGG 2 cut(s) 100, 369
BseMI GCAATG 1 cut(s) 793
BseMII CTCAG 3 cut(s) 171, 558, 628
BseXI GCAGC 1 cut(s) 367
BsiSI CCGG 1 cut(s) 468
BslI CCNNNNNNNGG 2 cut(s) 100, 369
BsmAI GTCTC 2 cut(s) 192, 245
Bsp13I TCCGGA 1 cut(s) 467
Bsp1407I TGTACA 1 cut(s) 715
Bsp143I GATC 3 cut(s) 118, 366, 442
Bsp1720I GCTNAGC 1 cut(s) 180
BspACI CCGC 2 cut(s) 184, 563
BspCNI CTCAG 3 cut(s) 172, 559, 627
BspEI TCCGGA 1 cut(s) 467
BspHI TCATGA 2 cut(s) 135, 414
BspLI GGNNCC 2 cut(s) 475, 598
BspMI ACCTGC 2 cut(s) 101, 295
BspPI GGATC 1 cut(s) 450
BsrBI CCGCTC 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 793
BsrGI TGTACA 1 cut(s) 715
BssECI CCNNGG 1 cut(s) 487
BssMI GATC 3 cut(s) 118, 366, 442
Bst2UI CCWGG 2 cut(s) 107, 489
Bst4CI ACNGT 4 cut(s) 55, 581, 636, 740
Bst6I CTCTTC 2 cut(s) 275, 356
BstAUI TGTACA 1 cut(s) 715
BstDEI CTNAG 4 cut(s) 180, 567, 614, 661
BstF5I GGATG 7 cut(s) 43, 90, 359, 378, 510, 608, 697
BstKTI GATC 3 cut(s) 121, 369, 445
BstMAI GTCTC 2 cut(s) 192, 245
BstMBI GATC 3 cut(s) 118, 366, 442
BstMWI GCNNNNNNNGC 2 cut(s) 224, 763
BstNI CCWGG 2 cut(s) 107, 489
BstNSI RCATGY 2 cut(s) 89, 776
BstSCI CCNGG 2 cut(s) 105, 487
BstV1I GCAGC 1 cut(s) 367
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
BsuI GTATCC 1 cut(s) 398
BtsCI GGATG 7 cut(s) 43, 90, 359, 378, 510, 608, 697
BtsIMutI CAGTG 1 cut(s) 586
BveI ACCTGC 2 cut(s) 101, 295
CaiI CAGNNNCTG 2 cut(s) 585, 634
CciI TCATGA 2 cut(s) 135, 414
Cfr13I GGNCC 1 cut(s) 103
Csp6I GTAC 2 cut(s) 632, 716
CviJI RGCY 7 cut(s) 218, 267, 358, 380, 474, 530, 597
CviKI_1 RGCY 7 cut(s) 218, 267, 358, 380, 474, 530, 597
CviQI GTAC 2 cut(s) 632, 716
DdeI CTNAG 4 cut(s) 180, 567, 614, 661
DpnI GATC 3 cut(s) 120, 368, 444
DpnII GATC 3 cut(s) 118, 366, 442
Eam1104I CTCTTC 2 cut(s) 275, 356
EarI CTCTTC 2 cut(s) 275, 356
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 363
EcoRII CCWGG 2 cut(s) 105, 487
EcoRV GATATC 1 cut(s) 433
EcoT22I ATGCAT 1 cut(s) 681
FauI CCCGC 1 cut(s) 177
FauNDI CATATG 1 cut(s) 783
Fnu4HI GCNGC 1 cut(s) 381
FokI GGATG 7 cut(s) 50, 77, 346, 385, 517, 595, 684
Fsp4HI GCNGC 1 cut(s) 381
FspBI CTAG 3 cut(s) 230, 248, 759
GluI GCNGC 1 cut(s) 381
HapII CCGG 1 cut(s) 468
HinfI GANTC 3 cut(s) 29, 46, 330
HpaII CCGG 1 cut(s) 468
HphI GGTGA 2 cut(s) 160, 728
Hpy188I TCNGA 2 cut(s) 60, 335
HpyAV CCTTC 6 cut(s) 62, 104, 199, 433, 631, 711
HpyCH4III ACNGT 4 cut(s) 55, 581, 636, 740
HpyCH4IV ACGT 1 cut(s) 154
HpyCH4V TGCA 5 cut(s) 646, 652, 679, 766, 798
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 763
HpyF3I CTNAG 4 cut(s) 180, 567, 614, 661
HpySE526I ACGT 1 cut(s) 154
Kpn2I TCCGGA 1 cut(s) 467
Kzo9I GATC 3 cut(s) 118, 366, 442
LmnI GCTCC 3 cut(s) 479, 535, 602
Lsp1109I GCAGC 1 cut(s) 367
LweI GCATC 3 cut(s) 118, 326, 617
MaeI CTAG 3 cut(s) 230, 248, 759
MaeII ACGT 1 cut(s) 154
MaeIII GTNAC 4 cut(s) 47, 158, 734, 775
MalI GATC 3 cut(s) 120, 368, 444
MbiI CCGCTC 1 cut(s) 184
MboI GATC 3 cut(s) 118, 366, 442
MboII GAAGA 2 cut(s) 292, 343
MfeI CAATTG 1 cut(s) 801
MflI RGATCY 1 cut(s) 442
MluCI AATT 5 cut(s) 189, 212, 290, 492, 801
MlyI GAGTC 2 cut(s) 23, 55
MmeI TCCRAC 1 cut(s) 649
MnlI CCTC 7 cut(s) 89, 143, 359, 464, 564, 587, 623
Mph1103I ATGCAT 1 cut(s) 681
MroI TCCGGA 1 cut(s) 467
MseI TTAA 2 cut(s) 729, 826
MslI CAYNNNNRTG 1 cut(s) 723
MspI CCGG 1 cut(s) 468
MspR9I CCNGG 2 cut(s) 107, 489
MunI CAATTG 1 cut(s) 801
MvaI CCWGG 2 cut(s) 107, 489
MwoI GCNNNNNNNGC 2 cut(s) 224, 763
NdeI CATATG 1 cut(s) 783
NdeII GATC 3 cut(s) 118, 366, 442
NlaIV GGNNCC 2 cut(s) 475, 598
NmuCI GTSAC 2 cut(s) 47, 734
NsiI ATGCAT 1 cut(s) 681
NspI RCATGY 2 cut(s) 89, 776
PagI TCATGA 2 cut(s) 135, 414
PciI ACATGT 1 cut(s) 772
PfeI GAWTC 1 cut(s) 330
PkrI GCNGC 1 cut(s) 382
PleI GAGTC 2 cut(s) 23, 54
PpsI GAGTC 2 cut(s) 23, 54
PscI ACATGT 1 cut(s) 772
Psp6I CCWGG 2 cut(s) 105, 487
PspGI CCWGG 2 cut(s) 105, 487
PspN4I GGNNCC 2 cut(s) 475, 598
PspPI GGNCC 1 cut(s) 103
PstNI CAGNNNCTG 2 cut(s) 585, 634
PsuI RGATCY 1 cut(s) 442
RsaI GTAC 2 cut(s) 633, 717
RsaNI GTAC 2 cut(s) 632, 716
RseI CAYNNNNRTG 1 cut(s) 723
SaqAI TTAA 2 cut(s) 729, 826
SatI GCNGC 1 cut(s) 381
Sau3AI GATC 3 cut(s) 118, 366, 442
Sau96I GGNCC 1 cut(s) 103
SchI GAGTC 2 cut(s) 23, 55
ScrFI CCNGG 2 cut(s) 107, 489
SfaNI GCATC 3 cut(s) 118, 326, 617
SinI GGWCC 1 cut(s) 103
SmiMI CAYNNNNRTG 1 cut(s) 723
Sse9I AATT 5 cut(s) 189, 212, 290, 492, 801
SsiI CCGC 2 cut(s) 184, 563
SspMI CTAG 3 cut(s) 230, 248, 759
StyD4I CCNGG 2 cut(s) 105, 487
TaaI ACNGT 4 cut(s) 55, 581, 636, 740
TaiI ACGT 1 cut(s) 157
TasI AATT 5 cut(s) 189, 212, 290, 492, 801
TatI WGTACW 1 cut(s) 715
TfiI GAWTC 1 cut(s) 330
Tru1I TTAA 2 cut(s) 729, 826
Tru9I TTAA 2 cut(s) 729, 826
TscAI CASTG 1 cut(s) 586
TseFI GTSAC 2 cut(s) 47, 734
TseI GCWGC 1 cut(s) 380
Tsp45I GTSAC 2 cut(s) 47, 734
TspDTI ATGAA 8 cut(s) 81, 152, 218, 252, 303, 408, 431, 557
TspRI CASTG 1 cut(s) 586
VpaK11BI GGWCC 1 cut(s) 103
XbaI TCTAGA 1 cut(s) 229
XceI RCATGY 2 cut(s) 89, 776
XcmI CCANNNNNNNNNTGG 2 cut(s) 97, 370
XspI CTAG 3 cut(s) 230, 248, 759
Zsp2I ATGCAT 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.