MD03G1095300.v1.1

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000536
Physical Location & Seq
Reverse (-)
8080006 .. 8083221
3216 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1095300.v1.1.491

Sequence Viewer

Length: 1134 bp
ATGGCCGAAGCTGAGGATATTCAACCACTCGTCTGTGACAATGGAACAGGAATGGTTAAGGCTGGATTTGCTGGAGATGATGCTCCAAGGGCTGTCTTTCCTAGCATTGTCGGCCGCCCACGTCACACTGGTGTGATGGTTGGAATGGGTCAAAAGGATGCATATGTTGGGGATGAGGCTCAGTCGAAGCGTGGTATCTTGACTCTGAAATACCCCATTGAGCATGGTATTGTGAGCAACTGGGATGATATGGAGAAGATTTGGCATCACACGTTCTACAATGAACTTCGTGTGGCCCCAGAAGAACATCCAGTTCTGCTCACTGAAGCACCTCTGAACCCAAAGGCTAATCGTGAGAAAATGACCCAAATCATGTTTGAGACATTCAACACTCCTGCTATGTATGTCGCCATCCAGGCTGTTCTTTCCCTCTACGCCAGTGGTCGTACAACTGGTATTGTTCTTGACTCTGGAGATGGTGTCAGTCACACTGTCCCCATTTATGAGGGATATGCTCTTCCCCATGCCATCCTCCGTCTTGACTTAGCAGGTCGTGACCTCACCGATGCCCTTATGAAAATTTTGACTGAGCGTGGGTATTCCTTCACCACCACAGCTGAGCGAGAAATTGTCAGGGATATGAAGGAGAAGCTTGCATATATTGCCCTCGATTATGAGCAGGAGCTTGAAACTTCCAAGACAAGTTCTTCTGTTGAGAAGAGCTATGAATTGCCTGATGGGCAGGTGATCACCATCGGTGCTGAGCGTTTCCGCTGCCCAGAAGTACTCTTCCAGCCATCCATGATTGGAATGGAAGCTGCAGGCATCCATGAGACCACATACAACTCTATCATGAAGTGCGACGTCGATATTAGGAAGGATTTGTATGGAAACATTGTCCTCAGTGGTGGTTCTACCATGTTCCCTGGCATTGCTGACAGAATGAGCAAGGAAATCACAGCTTTGGCACCTAGCAGCATGAAAATCAAGGTTGTTGCTCCACCCGAGAGGAAGTACAGTGTCTGGATCGGTGGATCCATCCTTGCATCTCTTAGTACTTTCCAGCAGATGTGGATCGCCAAGGCAGAGTACGACGAATCTGGCCCATCAATCGTACACAGGAAGTGTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

378

Amino Acids

41.7

Weight (kDa)

5.31

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 5 - 377 2.7e-146 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000536)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37620 AT2G37620 AT2G37620 AT2G37620 AT3G46520 AT3G46520 AT3G53750 AT3G53750 AT5G59370 AT5G59370
fragaria_vesca FvH4_3g35960 FvH4_6g13940 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_7g22410 FvH4_7g22410 FvH4_7g22410
malus_domestica MD03G1095300.v1.1 MD04G1127400.v1.1 MD11G1110300.v1.1 MD12G1056900.v1.1 MD12G1140800.v1.1 MD14G1056700.v1.1
prunus_persica Prupe.6G078800_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.7G077300_v2.0.a1 Prupe.7G077300_v2.0.a1
pyrus_communis pycom01g02640 pycom03g07780 pycom11g09110 pycom126g00040 pycom14g04650 pycom15g30330
rosa_chinensis RchiOBHm_Chr1g0367041 RchiOBHm_Chr3g0466761 RchiOBHm_Chr3g0479651 RchiOBHm_Chr5g0064311
rosa_laevigata RLG00000023550 RLG00000024555 RLG00000027304 RLG00000035715
rosa_multiflora Rmu_co8518979.1_g000001 Rmu_sc0002269.1_g000004 Rmu_sc0003467.1_g000022 Rmu_sc0003755.1_g000002 Rmu_sc0013440.1_g000001 Rmu_sc0013700.1_g000002 Rmu_sc0026659.1_g000001 Rmu_sc0037142.1_g000001
rosa_roxburghii Rroxscaffold_1G00016700 Rroxscaffold_4G00289680 Rroxscaffold_6G00402090
rosa_rugosa Rorug01G0334100 Rorug03G0083700 Rorug03G0083800 Rorug03G0177100 Rorug03G0177200 Rorug05G0364000 Rorug05G0364100
rosa_samantha Rh1AG340900 Rh1BG301400 Rh1CG317600 Rh1DG333800 Rh2AG276500 Rh3AG130800 Rh3AG227900 Rh3BG151800 Rh3BG261100 Rh3CG151700 Rh3DG152500 Rh3DG254400 Rh5AG421900 Rh5BG437600 Rh5CG460300 Rh5DG450700
rosa_wichuraiana Rw0G023870 Rw1G030280 Rw3G012360 Rw3G020390 Rw5G039710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 733
AatII GACGTC 1 cut(s) 867
Acc36I ACCTGC 2 cut(s) 539, 733
AccB1I GGYRCC 1 cut(s) 967
AciI CCGC 2 cut(s) 115, 772
AclWI GGATC 4 cut(s) 1029, 1034, 1042, 1082
AcoI YGGCCR 2 cut(s) 3, 112
AcsI RAATTY 1 cut(s) 579
AcuI CTGAAG 1 cut(s) 345
AcyI GRCGYC 1 cut(s) 864
AfaI GTAC 6 cut(s) 448, 786, 1016, 1057, 1091, 1116
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 3 cut(s) 23, 388, 689
AjiI CACGTC 1 cut(s) 122
AjnI CCWGG 2 cut(s) 414, 925
AleI CACNNNNGTG 2 cut(s) 129, 131
AluBI AGCT 7 cut(s) 11, 617, 652, 685, 723, 818, 962
AluI AGCT 7 cut(s) 11, 617, 652, 685, 723, 818, 962
Alw26I GTCTC 2 cut(s) 374, 827
AlwI GGATC 4 cut(s) 1029, 1034, 1042, 1082
AlwNI CAGNNNCTG 1 cut(s) 1023
Ama87I CYCGRG 1 cut(s) 1004
AoxI GGCC 4 cut(s) 3, 112, 294, 1102
ApeKI GCWGC 3 cut(s) 774, 818, 975
ApoI RAATTY 1 cut(s) 579
Asp700I GAANNNNTTC 1 cut(s) 1127
AspS9I GGNCC 2 cut(s) 295, 1103
AsuHPI GGTGA 4 cut(s) 553, 598, 742, 757
AvaI CYCGRG 1 cut(s) 1004
BamHI GGATCC 1 cut(s) 1034
BanI GGYRCC 1 cut(s) 967
BbvCI CCTCAGC 1 cut(s) 12
BbvI GCAGC 3 cut(s) 761, 805, 987
BccI CCATC 9 cut(s) 130, 419, 470, 536, 731, 761, 805, 1046, 1114
BciT130I CCWGG 2 cut(s) 416, 927
BclI TGATCA 1 cut(s) 747
BcoDI GTCTC 2 cut(s) 374, 827
BfaI CTAG 2 cut(s) 102, 972
BfmI CTRYAG 1 cut(s) 819
BfuAI ACCTGC 2 cut(s) 539, 733
BglI GCCNNNNNGGC 2 cut(s) 416, 739
BisI GCNGC 4 cut(s) 115, 775, 819, 976
BlpI GCTNAGC 2 cut(s) 618, 762
BlsI GCNGC 4 cut(s) 116, 776, 820, 977
BmcAI AGTACT 2 cut(s) 786, 1057
Bme1390I CCNGG 2 cut(s) 416, 927
BmeT110I CYCGRG 1 cut(s) 1004
BmgBI CACGTC 1 cut(s) 122
BmgT120I GGNCC 2 cut(s) 295, 1103
BmiI GGNNCC 3 cut(s) 297, 969, 1036
BmrFI CCNGG 2 cut(s) 416, 927
BmrI ACTGGG 1 cut(s) 250
BmsI GCATC 6 cut(s) 70, 148, 274, 556, 834, 1055
BmuI ACTGGG 1 cut(s) 250
BpmI CTGGAG 2 cut(s) 93, 492
Bpu10I CCTNAGC 1 cut(s) 12
Bpu1102I GCTNAGC 2 cut(s) 618, 762
BsaBI GATNNNNATC 2 cut(s) 752, 1073
BsaHI GRCGYC 1 cut(s) 864
BsaI GGTCTC 1 cut(s) 827
BsaJI CCNNGG 3 cut(s) 86, 925, 1080
BsaXI ACNNNNNCTCC 2 cut(s) 465, 495
Bse1I ACTGG 5 cut(s) 133, 245, 311, 438, 457
Bse3DI GCAATG 1 cut(s) 930
Bse8I GATNNNNATC 2 cut(s) 752, 1073
BseBI CCWGG 2 cut(s) 416, 927
BseDI CCNNGG 3 cut(s) 86, 925, 1080
BseGI GGATG 9 cut(s) 163, 178, 250, 307, 411, 528, 797, 825, 1038
BseJI GATNNNNATC 2 cut(s) 752, 1073
BseMI GCAATG 1 cut(s) 930
BseMII CTCAG 5 cut(s) 194, 579, 609, 753, 916
BseNI ACTGG 5 cut(s) 133, 245, 311, 438, 457
BseX3I CGGCCG 1 cut(s) 112
BseXI GCAGC 3 cut(s) 761, 805, 987
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 4 cut(s) 5, 114, 296, 1104
BshNI GGYRCC 1 cut(s) 967
BsiEI CGRYCG 1 cut(s) 115
BsiHKCI CYCGRG 1 cut(s) 1004
BslFI GGGAC 1 cut(s) 479
BsmAI GTCTC 2 cut(s) 374, 827
BsmFI GGGAC 1 cut(s) 479
BsnI GGCC 4 cut(s) 5, 114, 296, 1104
Bso31I GGTCTC 1 cut(s) 827
BsoBI CYCGRG 1 cut(s) 1004
Bsp143I GATC 4 cut(s) 747, 1026, 1034, 1074
Bsp1720I GCTNAGC 2 cut(s) 618, 762
BspACI CCGC 2 cut(s) 115, 772
BspANI GGCC 4 cut(s) 5, 114, 296, 1104
BspCNI CTCAG 6 cut(s) 4, 193, 580, 610, 754, 915
BspHI TCATGA 1 cut(s) 852
BspLI GGNNCC 3 cut(s) 297, 969, 1036
BspMAI CTGCAG 1 cut(s) 823
BspMI ACCTGC 2 cut(s) 539, 733
BspPI GGATC 4 cut(s) 1029, 1034, 1042, 1082
BspQI GCTCTTC 2 cut(s) 522, 713
BspT107I GGYRCC 1 cut(s) 967
BspTNI GGTCTC 1 cut(s) 827
BsrDI GCAATG 1 cut(s) 930
BsrI ACTGG 5 cut(s) 133, 245, 311, 438, 457
BssECI CCNNGG 3 cut(s) 86, 925, 1080
BssMI GATC 4 cut(s) 747, 1026, 1034, 1074
BssNI GRCGYC 1 cut(s) 864
BssT1I CCWWGG 2 cut(s) 86, 1080
Bst2UI CCWGG 2 cut(s) 416, 927
Bst4CI ACNGT 2 cut(s) 493, 1019
Bst6I CTCTTC 3 cut(s) 522, 713, 794
BstACI GRCGYC 1 cut(s) 864
BstAPI GCANNNNNTGC 1 cut(s) 662
BstC8I GCNNGC 2 cut(s) 654, 823
BstDEI CTNAG 8 cut(s) 12, 180, 544, 588, 618, 762, 902, 1052
BstF5I GGATG 9 cut(s) 163, 178, 250, 307, 411, 528, 797, 825, 1038
BstKTI GATC 4 cut(s) 750, 1029, 1037, 1077
BstMAI GTCTC 2 cut(s) 374, 827
BstMBI GATC 4 cut(s) 747, 1026, 1034, 1074
BstMCI CGRYCG 1 cut(s) 115
BstMWI GCNNNNNNNGC 6 cut(s) 68, 89, 111, 416, 662, 739
BstNI CCWGG 2 cut(s) 416, 927
BstSCI CCNGG 2 cut(s) 414, 925
BstSFI CTRYAG 1 cut(s) 819
BstV1I GCAGC 3 cut(s) 761, 805, 987
BstX2I RGATCY 1 cut(s) 1034
BstYI RGATCY 1 cut(s) 1034
BstZI CGGCCG 1 cut(s) 112
BsuRI GGCC 4 cut(s) 5, 114, 296, 1104
BtrI CACGTC 1 cut(s) 122
BtsCI GGATG 9 cut(s) 163, 178, 250, 307, 411, 528, 797, 825, 1038
BtsIMutI CAGTG 6 cut(s) 126, 321, 445, 489, 910, 1024
BveI ACCTGC 2 cut(s) 539, 733
Cac8I GCNNGC 2 cut(s) 654, 823
CaiI CAGNNNCTG 1 cut(s) 1023
CciI TCATGA 1 cut(s) 852
Cfr13I GGNCC 2 cut(s) 295, 1103
Csp6I GTAC 6 cut(s) 447, 785, 1015, 1056, 1090, 1115
CviAII CATG 8 cut(s) 224, 373, 524, 802, 830, 853, 919, 979
CviQI GTAC 6 cut(s) 447, 785, 1015, 1056, 1090, 1115
DdeI CTNAG 8 cut(s) 12, 180, 544, 588, 618, 762, 902, 1052
DpnI GATC 4 cut(s) 749, 1028, 1036, 1076
DpnII GATC 4 cut(s) 747, 1026, 1034, 1074
EaeI YGGCCR 2 cut(s) 3, 112
EagI CGGCCG 1 cut(s) 112
Eam1104I CTCTTC 3 cut(s) 522, 713, 794
EarI CTCTTC 3 cut(s) 522, 713, 794
EclXI CGGCCG 1 cut(s) 112
Eco130I CCWWGG 2 cut(s) 86, 1080
Eco31I GGTCTC 1 cut(s) 827
Eco52I CGGCCG 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 345
Eco88I CYCGRG 1 cut(s) 1004
EcoRII CCWGG 2 cut(s) 414, 925
EcoT14I CCWWGG 2 cut(s) 86, 1080
EcoT22I ATGCAT 1 cut(s) 163
ErhI CCWWGG 2 cut(s) 86, 1080
FaeI CATG 8 cut(s) 227, 376, 527, 805, 833, 856, 922, 982
FaqI GGGAC 1 cut(s) 479
FatI CATG 8 cut(s) 223, 372, 523, 801, 829, 852, 918, 978
FauNDI CATATG 1 cut(s) 163
FbaI TGATCA 1 cut(s) 747
Fnu4HI GCNGC 4 cut(s) 115, 775, 819, 976
FokI GGATG 9 cut(s) 170, 185, 257, 294, 398, 515, 784, 812, 1025
Fsp4HI GCNGC 4 cut(s) 115, 775, 819, 976
FspBI CTAG 2 cut(s) 102, 972
GluI GCNGC 4 cut(s) 115, 775, 819, 976
GsuI CTGGAG 2 cut(s) 93, 492
HaeIII GGCC 4 cut(s) 5, 114, 296, 1104
Hin1I GRCGYC 1 cut(s) 864
Hin1II CATG 8 cut(s) 227, 376, 527, 805, 833, 856, 922, 982
HindIII AAGCTT 1 cut(s) 650
HinfI GANTC 3 cut(s) 202, 467, 1097
HphI GGTGA 4 cut(s) 553, 598, 742, 757
Hpy166II GTNNAC 1 cut(s) 1117
Hpy188I TCNGA 2 cut(s) 207, 336
Hpy188III TCNNGA 8 cut(s) 199, 353, 464, 471, 539, 554, 853, 1024
Hpy8I GTNNAC 1 cut(s) 1117
Hpy99I CGWCG 3 cut(s) 866, 869, 1097
HpyAV CCTTC 3 cut(s) 613, 637, 871
HpyCH4III ACNGT 2 cut(s) 493, 1019
HpyCH4IV ACGT 3 cut(s) 121, 272, 864
HpyCH4V TGCA 4 cut(s) 161, 656, 821, 1046
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 89, 111, 416, 662, 739
HpyF3I CTNAG 8 cut(s) 12, 180, 544, 588, 618, 762, 902, 1052
HpySE526I ACGT 3 cut(s) 121, 272, 864
Hsp92I GRCGYC 1 cut(s) 864
Hsp92II CATG 8 cut(s) 227, 376, 527, 805, 833, 856, 922, 982
Ksp22I TGATCA 1 cut(s) 747
Kzo9I GATC 4 cut(s) 747, 1026, 1034, 1074
LguI GCTCTTC 2 cut(s) 522, 713
LmnI GCTCC 3 cut(s) 88, 682, 1003
Lsp1109I GCAGC 3 cut(s) 761, 805, 987
LweI GCATC 6 cut(s) 70, 148, 274, 556, 834, 1055
MaeI CTAG 2 cut(s) 102, 972
MaeII ACGT 3 cut(s) 121, 272, 864
MaeIII GTNAC 4 cut(s) 35, 122, 485, 554
MalI GATC 4 cut(s) 749, 1028, 1036, 1076
MboI GATC 4 cut(s) 747, 1026, 1034, 1074
MboII GAAGA 6 cut(s) 268, 314, 509, 699, 730, 781
MflI RGATCY 1 cut(s) 1034
MluCI AATT 3 cut(s) 579, 627, 728
MlyI GAGTC 2 cut(s) 196, 461
MmeI TCCRAC 1 cut(s) 121
Mph1103I ATGCAT 1 cut(s) 163
MroXI GAANNNNTTC 1 cut(s) 1127
MseI TTAA 1 cut(s) 57
MslI CAYNNNNRTG 2 cut(s) 129, 131
MspA1I CMGCKG 2 cut(s) 617, 774
MspR9I CCNGG 2 cut(s) 416, 927
MvaI CCWGG 2 cut(s) 416, 927
MwoI GCNNNNNNNGC 6 cut(s) 68, 89, 111, 416, 662, 739
NdeI CATATG 1 cut(s) 163
NdeII GATC 4 cut(s) 747, 1026, 1034, 1074
NlaIII CATG 8 cut(s) 227, 376, 527, 805, 833, 856, 922, 982
NlaIV GGNNCC 3 cut(s) 297, 969, 1036
NmuCI GTSAC 4 cut(s) 35, 122, 485, 554
NsiI ATGCAT 1 cut(s) 163
OliI CACNNNNGTG 2 cut(s) 129, 131
PagI TCATGA 1 cut(s) 852
PaqCI CACCTGC 1 cut(s) 733
PciSI GCTCTTC 2 cut(s) 522, 713
PdmI GAANNNNTTC 1 cut(s) 1127
PfeI GAWTC 1 cut(s) 1097
PkrI GCNGC 4 cut(s) 116, 776, 820, 977
PleI GAGTC 2 cut(s) 196, 461
PpsI GAGTC 2 cut(s) 196, 461
Psp6I CCWGG 2 cut(s) 414, 925
PspGI CCWGG 2 cut(s) 414, 925
PspN4I GGNNCC 3 cut(s) 297, 969, 1036
PspPI GGNCC 2 cut(s) 295, 1103
PstI CTGCAG 1 cut(s) 823
PstNI CAGNNNCTG 1 cut(s) 1023
PsuI RGATCY 1 cut(s) 1034
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 6 cut(s) 448, 786, 1016, 1057, 1091, 1116
RsaNI GTAC 6 cut(s) 447, 785, 1015, 1056, 1090, 1115
RseI CAYNNNNRTG 2 cut(s) 129, 131
SapI GCTCTTC 2 cut(s) 522, 713
SaqAI TTAA 1 cut(s) 57
SatI GCNGC 4 cut(s) 115, 775, 819, 976
Sau3AI GATC 4 cut(s) 747, 1026, 1034, 1074
Sau96I GGNCC 2 cut(s) 295, 1103
ScaI AGTACT 2 cut(s) 786, 1057
SchI GAGTC 2 cut(s) 196, 461
ScrFI CCNGG 2 cut(s) 416, 927
SfaNI GCATC 6 cut(s) 70, 148, 274, 556, 834, 1055
SfcI CTRYAG 1 cut(s) 819
SmiMI CAYNNNNRTG 2 cut(s) 129, 131
Sse9I AATT 3 cut(s) 579, 627, 728
SsiI CCGC 2 cut(s) 115, 772
SspMI CTAG 2 cut(s) 102, 972
StyD4I CCNGG 2 cut(s) 414, 925
StyI CCWWGG 2 cut(s) 86, 1080
TaaI ACNGT 2 cut(s) 493, 1019
TaiI ACGT 3 cut(s) 124, 275, 867
TaqI TCGA 3 cut(s) 185, 669, 867
TasI AATT 3 cut(s) 579, 627, 728
TatI WGTACW 3 cut(s) 784, 1014, 1055
TauI GCSGC 1 cut(s) 117
TfiI GAWTC 1 cut(s) 1097
Tru1I TTAA 1 cut(s) 57
Tru9I TTAA 1 cut(s) 57
TscAI CASTG 6 cut(s) 133, 328, 445, 496, 910, 1024
TseFI GTSAC 4 cut(s) 35, 122, 485, 554
TseI GCWGC 3 cut(s) 774, 818, 975
Tsp45I GTSAC 4 cut(s) 35, 122, 485, 554
TspDTI ATGAA 6 cut(s) 297, 590, 656, 741, 869, 995
TspGWI ACGGA 1 cut(s) 524
TspRI CASTG 6 cut(s) 133, 328, 445, 496, 910, 1024
XapI RAATTY 1 cut(s) 579
XcmI CCANNNNNNNNNTGG 1 cut(s) 808
XmnI GAANNNNTTC 1 cut(s) 1127
XspI CTAG 2 cut(s) 102, 972
ZraI GACGTC 1 cut(s) 865
ZrmI AGTACT 2 cut(s) 786, 1057
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.