Rh3BG261100

Actin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000536
Physical Location & Seq
Forward (+)
25299852 .. 25303474
3623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG261100.1

Sequence Viewer

Length: 825 bp
ATGCTAATGCTTTGCTGTTCAGGTATTGTTCTTGACTCTGGGGATGGTGTGAGTCACACAGTTCCGATATATGAAGGGTATGCCCTCCCACATGCCATCCTTCGTTTGGACCTGGCAGGTCGTGATCTTACAGATGCCCTCATGAAAATCTTGACTGAACGTGGTTACTCTTTCACCACTACTGCTGAGCGGGAAATTGTGAGAGACATGAAGGAGAAATTAGCCTATATTGCTCTAGACTATGAACAAGAACTAGAGACAGCAAAGACCAGCTCATCTGTTGAGAAGAGTTATGAATTACCTGATGGGCAGGTTATCACGATTGGTGCTGAAAGATTCCGATGCCCTGAAGTCCTCTTCCAGCCATCCATGATCGGGATGGAAGCTGCTGGTATCCATGAAACCACATACAACTCTATCATGAAGTGTGATGTTGATATCAGGAAGGATCTCTATGGCAACATTGTTCTTTCCGGAGGCTCCACTATGTTCCCTGGAATTGCTGATAGGATGAGCAAAGAGATTACAGCATTAGCTCCAAGTAGCATGAAAATCAAGGTCGTAGCACCGCCCGAGAGGAAATACAGTGTCTGGATAGGAGGCTCCATTTTGGCATCCCTCAGCACCTTCCAACAGGTAATGTGCTGCGTGCAAATGCACATCTGCTTAGGCACTCTACTAATGCATTTCTCGTTCAACTTCCATCCTTCCACGTCTTTTGTACATCTATATGTTAAAACTCACCGTAAGATGACTATAACGCCTAGTTTGCAGATACATGTTACACATGGCACACCATTGCACAATTTACTTGAAATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

274

Amino Acids

30.44

Weight (kDa)

6.11

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 7 - 214 2.3e-70 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000536)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37620 AT2G37620 AT2G37620 AT2G37620 AT3G46520 AT3G46520 AT3G53750 AT3G53750 AT5G59370 AT5G59370
fragaria_vesca FvH4_3g35960 FvH4_6g13940 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_6g22300 FvH4_7g22410 FvH4_7g22410 FvH4_7g22410
malus_domestica MD03G1095300.v1.1 MD04G1127400.v1.1 MD11G1110300.v1.1 MD12G1056900.v1.1 MD12G1140800.v1.1 MD14G1056700.v1.1
prunus_persica Prupe.6G078800_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.6G254100_v2.0.a1 Prupe.7G077300_v2.0.a1 Prupe.7G077300_v2.0.a1
pyrus_communis pycom01g02640 pycom03g07780 pycom11g09110 pycom126g00040 pycom14g04650 pycom15g30330
rosa_chinensis RchiOBHm_Chr1g0367041 RchiOBHm_Chr3g0466761 RchiOBHm_Chr3g0479651 RchiOBHm_Chr5g0064311
rosa_laevigata RLG00000023550 RLG00000024555 RLG00000027304 RLG00000035715
rosa_multiflora Rmu_co8518979.1_g000001 Rmu_sc0002269.1_g000004 Rmu_sc0003467.1_g000022 Rmu_sc0003755.1_g000002 Rmu_sc0013440.1_g000001 Rmu_sc0013700.1_g000002 Rmu_sc0026659.1_g000001 Rmu_sc0037142.1_g000001
rosa_roxburghii Rroxscaffold_1G00016700 Rroxscaffold_4G00289680 Rroxscaffold_6G00402090
rosa_rugosa Rorug01G0334100 Rorug03G0083700 Rorug03G0083800 Rorug03G0177100 Rorug03G0177200 Rorug05G0364000 Rorug05G0364100
rosa_samantha Rh1AG340900 Rh1BG301400 Rh1CG317600 Rh1DG333800 Rh2AG276500 Rh3AG130800 Rh3AG227900 Rh3BG151800 Rh3BG261100 Rh3CG151700 Rh3DG152500 Rh3DG254400 Rh5AG421900 Rh5BG437600 Rh5CG460300 Rh5DG450700
rosa_wichuraiana Rw0G023870 Rw1G030280 Rw3G012360 Rw3G020390 Rw5G039710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 107, 301
AccBSI CCGCTC 1 cut(s) 190
AccIII TCCGGA 1 cut(s) 473
AciI CCGC 2 cut(s) 190, 569
AclWI GGATC 1 cut(s) 456
AcuI CTGAAG 1 cut(s) 369
AfaI GTAC 1 cut(s) 723
AfiI CCNNNNNNNGG 2 cut(s) 106, 375
AflIII ACRYGT 1 cut(s) 778
AgsI TTSAA 2 cut(s) 697, 815
AjiI CACGTC 1 cut(s) 714
AjnI CCWGG 2 cut(s) 111, 493
AluBI AGCT 3 cut(s) 273, 386, 536
AluI AGCT 3 cut(s) 273, 386, 536
Alw26I GTCTC 2 cut(s) 198, 251
AlwI GGATC 1 cut(s) 456
AlwNI CAGNNNCTG 1 cut(s) 591
Ama87I CYCGRG 1 cut(s) 572
Aor13HI TCCGGA 1 cut(s) 473
ApeKI GCWGC 2 cut(s) 386, 645
AspS9I GGNCC 1 cut(s) 109
AsuHPI GGTGA 2 cut(s) 166, 734
AvaI CYCGRG 1 cut(s) 572
AvaII GGWCC 1 cut(s) 109
BbvCI CCTCAGC 1 cut(s) 620
BbvI GCAGC 2 cut(s) 373, 632
BccI CCATC 6 cut(s) 38, 104, 299, 373, 373, 711
BciT130I CCWGG 2 cut(s) 113, 495
BciVI GTATCC 1 cut(s) 404
BcoDI GTCTC 2 cut(s) 198, 251
BfaI CTAG 3 cut(s) 236, 254, 765
BfuAI ACCTGC 2 cut(s) 107, 301
BfuI GTATCC 1 cut(s) 404
BisI GCNGC 2 cut(s) 387, 646
BlpI GCTNAGC 1 cut(s) 186
BlsI GCNGC 2 cut(s) 388, 647
Bme1390I CCNGG 2 cut(s) 113, 495
Bme18I GGWCC 1 cut(s) 109
BmeT110I CYCGRG 1 cut(s) 572
BmgBI CACGTC 1 cut(s) 714
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 2 cut(s) 481, 604
BmrFI CCNGG 2 cut(s) 113, 495
BmsI GCATC 3 cut(s) 124, 332, 623
Bpu10I CCTNAGC 2 cut(s) 620, 667
Bpu1102I GCTNAGC 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 493
BsaWI WCCGGW 1 cut(s) 473
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 375
Bse3DI GCAATG 1 cut(s) 797
BseAI TCCGGA 1 cut(s) 473
BseBI CCWGG 2 cut(s) 113, 495
BseDI CCNNGG 1 cut(s) 493
BseGI GGATG 7 cut(s) 49, 96, 365, 384, 516, 614, 703
BseLI CCNNNNNNNGG 2 cut(s) 106, 375
BseMI GCAATG 1 cut(s) 797
BseMII CTCAG 2 cut(s) 177, 634
BseXI GCAGC 2 cut(s) 373, 632
BsiHKCI CYCGRG 1 cut(s) 572
BsiSI CCGG 1 cut(s) 474
BslI CCNNNNNNNGG 2 cut(s) 106, 375
BsmAI GTCTC 2 cut(s) 198, 251
BsoBI CYCGRG 1 cut(s) 572
Bsp13I TCCGGA 1 cut(s) 473
Bsp1407I TGTACA 1 cut(s) 721
Bsp143I GATC 3 cut(s) 124, 372, 448
Bsp1720I GCTNAGC 1 cut(s) 186
BspACI CCGC 2 cut(s) 190, 569
BspCNI CTCAG 2 cut(s) 178, 633
BspEI TCCGGA 1 cut(s) 473
BspHI TCATGA 2 cut(s) 141, 420
BspLI GGNNCC 2 cut(s) 481, 604
BspMI ACCTGC 2 cut(s) 107, 301
BspPI GGATC 1 cut(s) 456
BsrBI CCGCTC 1 cut(s) 190
BsrDI GCAATG 1 cut(s) 797
BsrGI TGTACA 1 cut(s) 721
BssECI CCNNGG 1 cut(s) 493
BssMI GATC 3 cut(s) 124, 372, 448
Bst2UI CCWGG 2 cut(s) 113, 495
Bst4CI ACNGT 3 cut(s) 61, 587, 746
Bst6I CTCTTC 2 cut(s) 281, 362
BstAUI TGTACA 1 cut(s) 721
BstC8I GCNNGC 1 cut(s) 650
BstDEI CTNAG 3 cut(s) 186, 620, 667
BstF5I GGATG 7 cut(s) 49, 96, 365, 384, 516, 614, 703
BstKTI GATC 3 cut(s) 127, 375, 451
BstMAI GTCTC 2 cut(s) 198, 251
BstMBI GATC 3 cut(s) 124, 372, 448
BstMWI GCNNNNNNNGC 2 cut(s) 230, 769
BstNI CCWGG 2 cut(s) 113, 495
BstNSI RCATGY 2 cut(s) 95, 782
BstSCI CCNGG 2 cut(s) 111, 493
BstV1I GCAGC 2 cut(s) 373, 632
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BsuI GTATCC 1 cut(s) 404
BtrI CACGTC 1 cut(s) 714
BtsCI GGATG 7 cut(s) 49, 96, 365, 384, 516, 614, 703
BtsIMutI CAGTG 1 cut(s) 592
BveI ACCTGC 2 cut(s) 107, 301
Cac8I GCNNGC 1 cut(s) 650
CaiI CAGNNNCTG 1 cut(s) 591
CciI TCATGA 2 cut(s) 141, 420
Cfr13I GGNCC 1 cut(s) 109
Csp6I GTAC 1 cut(s) 722
CspCI CAANNNNNGTGG 2 cut(s) 700, 735
CviJI RGCY 7 cut(s) 224, 273, 364, 386, 480, 536, 603
CviKI_1 RGCY 7 cut(s) 224, 273, 364, 386, 480, 536, 603
CviQI GTAC 1 cut(s) 722
DdeI CTNAG 3 cut(s) 186, 620, 667
DpnI GATC 3 cut(s) 126, 374, 450
DpnII GATC 3 cut(s) 124, 372, 448
Eam1104I CTCTTC 2 cut(s) 281, 362
EarI CTCTTC 2 cut(s) 281, 362
Eco32I GATATC 1 cut(s) 439
Eco47I GGWCC 1 cut(s) 109
Eco57I CTGAAG 1 cut(s) 369
Eco88I CYCGRG 1 cut(s) 572
EcoRII CCWGG 2 cut(s) 111, 493
EcoRV GATATC 1 cut(s) 439
EcoT22I ATGCAT 1 cut(s) 687
FauI CCCGC 1 cut(s) 183
Fnu4HI GCNGC 2 cut(s) 387, 646
FokI GGATG 7 cut(s) 56, 83, 352, 391, 523, 601, 690
Fsp4HI GCNGC 2 cut(s) 387, 646
FspBI CTAG 3 cut(s) 236, 254, 765
GluI GCNGC 2 cut(s) 387, 646
HapII CCGG 1 cut(s) 474
HinfI GANTC 3 cut(s) 35, 52, 336
HpaII CCGG 1 cut(s) 474
HphI GGTGA 2 cut(s) 166, 734
Hpy188I TCNGA 2 cut(s) 66, 341
HpyAV CCTTC 6 cut(s) 68, 110, 205, 439, 637, 717
HpyCH4III ACNGT 3 cut(s) 61, 587, 746
HpyCH4IV ACGT 2 cut(s) 160, 713
HpyCH4V TGCA 5 cut(s) 652, 658, 685, 772, 802
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 769
HpyF3I CTNAG 3 cut(s) 186, 620, 667
HpySE526I ACGT 2 cut(s) 160, 713
Kpn2I TCCGGA 1 cut(s) 473
Kzo9I GATC 3 cut(s) 124, 372, 448
LmnI GCTCC 3 cut(s) 485, 541, 608
Lsp1109I GCAGC 2 cut(s) 373, 632
LweI GCATC 3 cut(s) 124, 332, 623
MaeI CTAG 3 cut(s) 236, 254, 765
MaeII ACGT 2 cut(s) 160, 713
MaeIII GTNAC 3 cut(s) 53, 164, 781
MalI GATC 3 cut(s) 126, 374, 450
MbiI CCGCTC 1 cut(s) 190
MboI GATC 3 cut(s) 124, 372, 448
MboII GAAGA 2 cut(s) 298, 349
MflI RGATCY 1 cut(s) 448
MluCI AATT 5 cut(s) 195, 218, 296, 498, 805
MlyI GAGTC 2 cut(s) 29, 61
MmeI TCCRAC 1 cut(s) 655
MnlI CCTC 7 cut(s) 95, 149, 365, 470, 570, 593, 629
Mph1103I ATGCAT 1 cut(s) 687
MroI TCCGGA 1 cut(s) 473
MseI TTAA 1 cut(s) 735
MslI CAYNNNNRTG 1 cut(s) 729
MspI CCGG 1 cut(s) 474
MspR9I CCNGG 2 cut(s) 113, 495
MvaI CCWGG 2 cut(s) 113, 495
MwoI GCNNNNNNNGC 2 cut(s) 230, 769
NdeII GATC 3 cut(s) 124, 372, 448
NlaIV GGNNCC 2 cut(s) 481, 604
NmuCI GTSAC 1 cut(s) 53
NsiI ATGCAT 1 cut(s) 687
NspI RCATGY 2 cut(s) 95, 782
PagI TCATGA 2 cut(s) 141, 420
PciI ACATGT 1 cut(s) 778
PfeI GAWTC 1 cut(s) 336
PkrI GCNGC 2 cut(s) 388, 647
PleI GAGTC 2 cut(s) 29, 60
PpsI GAGTC 2 cut(s) 29, 60
PscI ACATGT 1 cut(s) 778
Psp6I CCWGG 2 cut(s) 111, 493
PspGI CCWGG 2 cut(s) 111, 493
PspN4I GGNNCC 2 cut(s) 481, 604
PspPI GGNCC 1 cut(s) 109
PstNI CAGNNNCTG 1 cut(s) 591
PsuI RGATCY 1 cut(s) 448
RsaI GTAC 1 cut(s) 723
RsaNI GTAC 1 cut(s) 722
RseI CAYNNNNRTG 1 cut(s) 729
SaqAI TTAA 1 cut(s) 735
SatI GCNGC 2 cut(s) 387, 646
Sau3AI GATC 3 cut(s) 124, 372, 448
Sau96I GGNCC 1 cut(s) 109
SchI GAGTC 2 cut(s) 29, 61
ScrFI CCNGG 2 cut(s) 113, 495
SfaNI GCATC 3 cut(s) 124, 332, 623
SinI GGWCC 1 cut(s) 109
SmiMI CAYNNNNRTG 1 cut(s) 729
Sse9I AATT 5 cut(s) 195, 218, 296, 498, 805
SsiI CCGC 2 cut(s) 190, 569
SspMI CTAG 3 cut(s) 236, 254, 765
StyD4I CCNGG 2 cut(s) 111, 493
TaaI ACNGT 3 cut(s) 61, 587, 746
TaiI ACGT 2 cut(s) 163, 716
TasI AATT 5 cut(s) 195, 218, 296, 498, 805
TatI WGTACW 1 cut(s) 721
TfiI GAWTC 1 cut(s) 336
Tru1I TTAA 1 cut(s) 735
Tru9I TTAA 1 cut(s) 735
TscAI CASTG 1 cut(s) 592
TseFI GTSAC 1 cut(s) 53
TseI GCWGC 2 cut(s) 386, 645
Tsp45I GTSAC 1 cut(s) 53
TspDTI ATGAA 8 cut(s) 87, 158, 224, 258, 309, 414, 437, 563
TspRI CASTG 1 cut(s) 592
VpaK11BI GGWCC 1 cut(s) 109
XbaI TCTAGA 1 cut(s) 235
XceI RCATGY 2 cut(s) 95, 782
XcmI CCANNNNNNNNNTGG 2 cut(s) 103, 376
XspI CTAG 3 cut(s) 236, 254, 765
Zsp2I ATGCAT 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.