Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0009672

Physical Location & Seq

Genomic Coordinates Reverse (-)

9730908 .. 9736252

Gene Structure 5345 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1110200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2481 bp

ATGGAGCTCGGACAGCCTTCGTATCCGCACCATGGCCCGGTGGCTGAATCCCCTTCTGGGTTCTCTTCTCCGGTGAGTTTCCGACGTGGGTTTGACCGGCCTGCGACTTTCTCCTCTCAGTCTCAGTTGCCCGAGATTTTTGAAGAAGGTGGTGGTGGTGACAAGGTGCACGTGGCGGTTGGGAAGTCTGTGGAGAAAGCTTTGAGCTTGCTTCGTTGGACGGTCAAGCACTTTGGCTGTAATGAAATTTGTATACTTCATGTTCATCAGCCATCTCCGCTCATTCCCACTCTCTTAGGAAAACTACCCGCAAGTCAGGCAAATGACGAGGTGGTGACGGCTTTCAGGAGGGAGGAAAGGGAACGGAAAATGAAGCTTTTGCAAAATTATTTGAGGGCTTGCTCTAGAGCAAAGGTTAAAGCAAGCATTGTTATGGTTGAAGCTAATGAAATTCATAAAGGAATTGTAGAGTTGGTCAACAATAATGGGGTTAGGAAGCTTGTTATGGGAGCTGTACCAGAAGATTGCATGAAGGTGAAAAAGAGCTCCAGAAAAGCAAATTATGTAGCCAAATGTGCTCCAGTGTTCTGTGAAATACGGTTTATCAACAAAGGGAAACATGTCTGGACGAGAGATGCTTCAGATGGCCAAAGTTCTCTGACCTCATGTAGTCAACTGCAGATTGCACCTGCAGAAATTTTGAGTTCTAGATCATTTCAAAATAGAGAGAATGAAGAAATCCACGAAGAATTGGCTGCATCACCAACTTTAGCTTGTTCTTCAAATACATGCTTACCCTACAATATTCATAATTCAACTAGTACAAATTCTAGCTCTGGTTCAGGATATAATTCTGCTGAGGGAAGGATGTCTTCAGATTCTGATTTAAACGTTGAGGAACAGAGCTTATGCCGTATGCTTACGGAAGCTACACTAGAAGCCGAGGCATCAAGGGACAAGGCATTTGCAGAGCTATTGAAGCGCAAAAGTTTAGAATCAGAAGCTGTGAATGCTATCAACAAGGCCAAGGCATTTGAGTTAGCCCGTGGCCGAGAAGTTAAACTTAGAGAAGAGGCTGAGGACGCCTTGAGAACCACGATTGAGGAACAACAAAAACTCTTGGAAGAAAAGGAAGAAATTAGTGGTGAGATAAGCAGGGCCATGAGAAATATTGCCCTTCTAGACAGCCGAGCACAAGAAGCAAGTCGAAGGTTTGCTGAAGCTTCTGGGGAAGTGAAGCAAATTCAGACTTCGATTGCAACTCTTCAGCAGGAAAAACAGTGTATTCAACGACAAAAGGTGGAAGCCCTTCGCTGGATTGAGCGGTGGAGGAATCATCGACAAGCTGGAGCCGCAAACTGCAATGCACTCATTGGGTTTGTTGAAGAACTGCCGAAATTAGCAGAATTCTCATTGTCAGATTTGCAGACTGCAACATGCAATTTTTCTGAGAGCTTTAAAATTGGGCAGGGAGGATATGGTTGTGTTTATAAGGGGGAAATGCTGGGCAGAACTGTCGCCATAAGGAAATTGCATCCGCATAACTTGCAAGAACAAGCCGAGTTTCAACAAGAGGTACAAGTTCTTGGACAATTACAGCATCGTCACCTTGTGACCTTGCTTGGTGTTTGTCCAGAAGCCTGGTCGCTTGTATACGAATACTTATCAAATGGGAGTCTGCAAGACCATCTTTTCAGGAAAAGCAATATTTCTCCGGTGCCATGGAAGACCCGAACACGGATAATTGCTCAAATCTCTAGTGCCCTTTGCTTCTTGCACTCCTCTAGACCTGAAAAGATTGTCCATGGTGATTTGAAGCTGCAAAACATACTACTAGATTCTGAACTCAACTGCAAGATTTGTGATTTTGGGATTTGCAGGCTGGTCACTGAAGACAATCTTTACTGTCGAAGTTTCCGCTGGAGAACTGAGCCAAAGGGTGCTTTTCCTTACACAGATCCAGAGCTGCAGAGAGTGGGGGTCCTGACGCCCAAGTCTGATATTTATTCCTTTGGGCTGATCCTTCTGCAGTTAGTCACCGGACGGCCTCCAGTTGGCGTAGCAAGTGAGGTGCGCAAGGCAGTTTTAGGTGGAAATTTGACATCGATTATAGATTCCTCAGCTGGAGAATGGCCTATATCTGTGGCAACGCGATTGGTAGACCTTGGTTTGCAATGCTGTGAATTGAATAGTAGGGAAAGACCAGAGCTTACGCCAGCTTTAGTGAGGGAATTGCAGCAGCTACATGCCTCTGAAGAGCGACCAGTGCCATCTTTCTTCTTGTGTCCGATCCTTCAGGAAATAATGCATGACCCGCAGTTGGCAGCTGATGGCTTCACGTACGAAGGTGAAGCTTTGCGTGGATGGTTACAAAATTGTCGCGAGACTTCTCCAATGACCAATTTGAAATTGAGTCACTTACATCTCACTCCCAACCATGCCTTACGCCTTGCTATTCAAGATTGGCTTTGCAAGACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr U-box Usp

Protein Analysis

827

Amino Acids

92.25

Weight (kDa)

7.08

Isoelectric Point (pI)

61.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	485 - 740	3.3e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	487 - 738	2.3e-40	Protein tyrosine and serine/threonine kinase
U-box	PF04564	756 - 825	6.5e-17	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009672)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g35970 FvH4_3g35970 FvH4_3g35970 FvH4_3g35970 FvH4_3g35970
malus_domestica	MD03G1095200.v1.1 MD11G1110200.v1.1
prunus_persica	Prupe.6G078700_v2.0.a1 Prupe.6G078700_v2.0.a1
pyrus_communis	pycom03g07770 pycom11g09100
rosa_chinensis	RchiOBHm_Chr5g0064331
rosa_laevigata	RLG00000035717
rosa_multiflora	Rmu_sc0000806.1_g000029
rosa_roxburghii	Rroxscaffold_1G00016670
rosa_rugosa	Rorug05G0364100 Rorug05G0364100
rosa_samantha	Rh5AG422200 Rh5BG438100 Rh5CG460600 Rh5DG451000
rosa_wichuraiana	Rw5G039740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1491
AarI	CACCTGC	1 cut(s)	697
AasI	GACNNNNNNGTC	1 cut(s)	1993
Acc16I	TGCGCA	1 cut(s)	2075
Acc36I	ACCTGC	1 cut(s)	697
AccB1I	GGYRCC	1 cut(s)	1717
AccBSI	CCGCTC	2 cut(s)	280, 1324
AccI	GTMKAC	3 cut(s)	253, 1653, 2160
AccII	CGCG	2 cut(s)	2152, 2382
AciI	CCGC	9 cut(s)	26, 176, 278, 309, 1324, 1353, 1538, 1918, 2315
AclI	AACGTT	1 cut(s)	891
AclWI	GGATC	3 cut(s)	1952, 2014, 2284
AcoI	YGGCCR	2 cut(s)	646, 1048
AcsI	RAATTY	7 cut(s)	246, 450, 696, 826, 1242, 1406, 2095
AcuI	CTGAAG	7 cut(s)	624, 858, 1239, 1250, 1911, 2274, 2279
AcvI	CACGTG	1 cut(s)	172
AcyI	GRCGYC	2 cut(s)	1083, 1988
AdeI	CACNNNGTG	1 cut(s)	1612
AfaI	GTAC	4 cut(s)	516, 823, 1578, 2342
AfiI	CCNNNNNNNGG	7 cut(s)	32, 37, 57, 1314, 1737, 2054, 2320
AflIII	ACRYGT	1 cut(s)	619
AhlI	ACTAGT	1 cut(s)	818
AjiI	CACGTC	1 cut(s)	86
AjnI	CCWGG	1 cut(s)	1640
Alw21I	GWGCWC	5 cut(s)	9, 171, 548, 580, 1195
Alw26I	GTCTC	2 cut(s)	126, 2378
Alw44I	GTGCAC	1 cut(s)	167
AlwI	GGATC	3 cut(s)	1952, 2014, 2284
AlwNI	CAGNNNCTG	2 cut(s)	881, 1004
Ama87I	CYCGRG	1 cut(s)	131
AoxI	GGCC	8 cut(s)	34, 98, 646, 1023, 1048, 1158, 2045, 2132
ApaLI	GTGCAC	1 cut(s)	167
ApeKI	GCWGC	6 cut(s)	755, 1819, 1966, 2236, 2239, 2324
ApoI	RAATTY	7 cut(s)	246, 450, 696, 826, 1242, 1406, 2095
ArsI	GACNNNNNNTTYG	2 cut(s)	947, 979
Asp700I	GAANNNNTTC	1 cut(s)	1308
AspLEI	GCGC	2 cut(s)	984, 2076
AspS9I	GGNCC	3 cut(s)	35, 1158, 1981
AsuC2I	CCSGG	1 cut(s)	38
AvaI	CYCGRG	1 cut(s)	131
AvaII	GGWCC	1 cut(s)	1981
BaeGI	GKGCMC	2 cut(s)	171, 1765
BalI	TGGCCA	1 cut(s)	648
BanI	GGYRCC	1 cut(s)	1717
BanII	GRGCYC	2 cut(s)	9, 548
BbrPI	CACGTG	1 cut(s)	172
BbsI	GAAGAC	3 cut(s)	864, 1733, 1899
Bbv12I	GWGCWC	5 cut(s)	9, 171, 548, 580, 1195
BbvCI	CCTCAGC	3 cut(s)	858, 1077, 2119
BbvI	GCAGC	6 cut(s)	742, 1806, 1953, 2248, 2251, 2336
BccI	CCATC	6 cut(s)	280, 638, 1695, 2278, 2324, 2358
BceAI	ACGGC	3 cut(s)	354, 897, 2060
BcgI	CGANNNNNNTGC	2 cut(s)	1498, 1532
BciT130I	CCWGG	1 cut(s)	1642
BciVI	GTATCC	1 cut(s)	33
BcnI	CCSGG	1 cut(s)	38
BcoDI	GTCTC	2 cut(s)	126, 2378
BcuI	ACTAGT	1 cut(s)	818
BfaI	CTAG	9 cut(s)	405, 708, 819, 831, 935, 1181, 1758, 1785, 1835
BfmI	CTRYAG	4 cut(s)	677, 690, 1967, 2027
BfuAI	ACCTGC	1 cut(s)	697
BfuI	GTATCC	1 cut(s)	33
BisI	GCNGC	7 cut(s)	756, 1353, 1820, 1967, 2237, 2240, 2325
BlsI	GCNGC	7 cut(s)	757, 1354, 1821, 1968, 2238, 2241, 2326
Bme1390I	CCNGG	2 cut(s)	38, 1642
Bme18I	GGWCC	1 cut(s)	1981
BmeT110I	CYCGRG	1 cut(s)	131
BmgBI	CACGTC	1 cut(s)	86
BmgT120I	GGNCC	3 cut(s)	35, 1158, 1981
BmiI	GGNNCC	3 cut(s)	1351, 1719, 1982
BmrFI	CCNGG	2 cut(s)	38, 1642
BmsI	GCATC	5 cut(s)	625, 767, 956, 1543, 1609
BpiI	GAAGAC	3 cut(s)	864, 1733, 1899
BpmI	CTGGAG	6 cut(s)	532, 564, 1368, 1942, 2034, 2145
Bpu10I	CCTNAGC	3 cut(s)	858, 1077, 2119
BpuEI	CTTGAG	1 cut(s)	1108
BpuMI	CCSGG	1 cut(s)	38
Bsa29I	ATCGAT	1 cut(s)	2105
BsaAI	YACGTR	2 cut(s)	172, 2340
BsaHI	GRCGYC	2 cut(s)	1083, 1988
BsaJI	CCNNGG	7 cut(s)	31, 942, 1026, 1045, 1721, 1804, 2164
BsaWI	WCCGGW	3 cut(s)	70, 1714, 2039
Bsc4I	CCNNNNNNNGG	7 cut(s)	32, 37, 57, 1314, 1737, 2054, 2320
Bse118I	RCCGGY	1 cut(s)	96
Bse1I	ACTGG	3 cut(s)	581, 2051, 2264
Bse3DI	GCAATG	2 cut(s)	1369, 2180
BseBI	CCWGG	1 cut(s)	1642
BseCI	ATCGAT	1 cut(s)	2105
BseDI	CCNNGG	7 cut(s)	31, 942, 1026, 1045, 1721, 1804, 2164
BseGI	GGATG	3 cut(s)	873, 1534, 2369
BseLI	CCNNNNNNNGG	7 cut(s)	32, 37, 57, 1314, 1737, 2054, 2320
BseMI	GCAATG	2 cut(s)	1369, 2180
BseMII	CTCAG	7 cut(s)	131, 137, 849, 1068, 1440, 1920, 2133
BseNI	ACTGG	3 cut(s)	581, 2051, 2264
BseRI	GAGGAG	2 cut(s)	103, 1771
BseSI	GKGCMC	2 cut(s)	171, 1765
BseXI	GCAGC	6 cut(s)	742, 1806, 1953, 2248, 2251, 2336
BseYI	CCCAGC	1 cut(s)	1504
Bsh1236I	CGCG	2 cut(s)	2152, 2382
BshFI	GGCC	8 cut(s)	36, 100, 648, 1025, 1050, 1160, 2047, 2134
BshNI	GGYRCC	1 cut(s)	1717
BshVI	ATCGAT	1 cut(s)	2105
BsiHKAI	GWGCWC	5 cut(s)	9, 171, 548, 580, 1195
BsiHKCI	CYCGRG	1 cut(s)	131
BsiSI	CCGG	5 cut(s)	38, 71, 97, 1715, 2040
BsiWI	CGTACG	1 cut(s)	2340
BslFI	GGGAC	1 cut(s)	968
BslI	CCNNNNNNNGG	7 cut(s)	32, 37, 57, 1314, 1737, 2054, 2320
BsmAI	GTCTC	2 cut(s)	126, 2378
BsmFI	GGGAC	1 cut(s)	968
BsmI	GAATGC	1 cut(s)	1015
BsnI	GGCC	8 cut(s)	36, 100, 648, 1025, 1050, 1160, 2047, 2134
BsoBI	CYCGRG	1 cut(s)	131
Bsp1286I	GDGCHC	6 cut(s)	9, 171, 548, 580, 1195, 1765
Bsp143I	GATC	4 cut(s)	710, 1957, 2019, 2289
Bsp19I	CCATGG	3 cut(s)	31, 1721, 1804
Bsp68I	TCGCGA	1 cut(s)	2382
BspACI	CCGC	9 cut(s)	26, 176, 278, 309, 1324, 1353, 1538, 1918, 2315
BspANI	GGCC	8 cut(s)	36, 100, 648, 1025, 1050, 1160, 2047, 2134
BspCNI	CTCAG	7 cut(s)	130, 136, 850, 1069, 1441, 1921, 2132
BspDI	ATCGAT	1 cut(s)	2105
BspFNI	CGCG	2 cut(s)	2152, 2382
BspLI	GGNNCC	3 cut(s)	1351, 1719, 1982
BspMAI	CTGCAG	4 cut(s)	681, 694, 1971, 2031
BspMI	ACCTGC	1 cut(s)	697
BspPI	GGATC	3 cut(s)	1952, 2014, 2284
BspQI	GCTCTTC	1 cut(s)	2250
BspT107I	GGYRCC	1 cut(s)	1717
BsrBI	CCGCTC	2 cut(s)	280, 1324
BsrDI	GCAATG	2 cut(s)	1369, 2180
BsrFI	RCCGGY	1 cut(s)	96
BsrI	ACTGG	3 cut(s)	581, 2051, 2264
BssAI	RCCGGY	1 cut(s)	96
BssECI	CCNNGG	7 cut(s)	31, 942, 1026, 1045, 1721, 1804, 2164
BssMI	GATC	4 cut(s)	710, 1957, 2019, 2289
BssNAI	GTATAC	2 cut(s)	254, 1654
BssNI	GRCGYC	2 cut(s)	1083, 1988
BssT1I	CCWWGG	5 cut(s)	31, 1026, 1721, 1804, 2164
Bst1107I	GTATAC	2 cut(s)	254, 1654
Bst2UI	CCWGG	1 cut(s)	1642
Bst4CI	ACNGT	5 cut(s)	223, 600, 1281, 1516, 1907
Bst6I	CTCTTC	4 cut(s)	70, 1065, 1269, 2250
BstACI	GRCGYC	2 cut(s)	1083, 1988
BstAPI	GCANNNNNTGC	1 cut(s)	1546
BstBAI	YACGTR	2 cut(s)	172, 2340
BstC8I	GCNNGC	6 cut(s)	102, 209, 400, 424, 1880, 2217
BstDEI	CTNAG	9 cut(s)	117, 123, 295, 858, 1064, 1077, 1449, 1929, 2119
BstDSI	CCRYGG	4 cut(s)	31, 1045, 1721, 1804
BstF5I	GGATG	3 cut(s)	873, 1534, 2369
BstFNI	CGCG	2 cut(s)	2152, 2382
BstHHI	GCGC	2 cut(s)	984, 2076
BstKTI	GATC	4 cut(s)	713, 1960, 2022, 2292
BstMAI	GTCTC	2 cut(s)	126, 2378
BstMBI	GATC	4 cut(s)	710, 1957, 2019, 2289
BstNI	CCWGG	1 cut(s)	1642
BstNSI	RCATGY	4 cut(s)	623, 792, 1440, 2249
BstSCI	CCNGG	2 cut(s)	36, 1640
BstSFI	CTRYAG	4 cut(s)	677, 690, 1967, 2027
BstSLI	GKGCMC	2 cut(s)	171, 1765
BstUI	CGCG	2 cut(s)	2152, 2382
BstV1I	GCAGC	6 cut(s)	742, 1806, 1953, 2248, 2251, 2336
BstV2I	GAAGAC	3 cut(s)	864, 1733, 1899
BstX2I	RGATCY	1 cut(s)	1957
BstXI	CCANNNNNNTGG	1 cut(s)	1641
BstYI	RGATCY	1 cut(s)	1957
BstZ17I	GTATAC	2 cut(s)	254, 1654
Bsu15I	ATCGAT	1 cut(s)	2105
BsuI	GTATCC	1 cut(s)	33
BsuRI	GGCC	8 cut(s)	36, 100, 648, 1025, 1050, 1160, 2047, 2134
BsuTUI	ATCGAT	1 cut(s)	2105
BtgI	CCRYGG	4 cut(s)	31, 1045, 1721, 1804
BtrI	CACGTC	1 cut(s)	86
BtsCI	GGATG	3 cut(s)	873, 1534, 2369
BtsIMutI	CAGTG	4 cut(s)	588, 1286, 1887, 2271
BtuMI	TCGCGA	1 cut(s)	2382
BveI	ACCTGC	1 cut(s)	697
Cac8I	GCNNGC	6 cut(s)	102, 209, 400, 424, 1880, 2217
CaiI	CAGNNNCTG	2 cut(s)	881, 1004
CfoI	GCGC	2 cut(s)	984, 2076
Cfr10I	RCCGGY	1 cut(s)	96
Cfr13I	GGNCC	3 cut(s)	35, 1158, 1981
ClaI	ATCGAT	1 cut(s)	2105
CseI	GACGC	2 cut(s)	1091, 1996
Csp6I	GTAC	4 cut(s)	515, 822, 1577, 2341
CviQI	GTAC	4 cut(s)	515, 822, 1577, 2341
DdeI	CTNAG	9 cut(s)	117, 123, 295, 858, 1064, 1077, 1449, 1929, 2119
DpnI	GATC	4 cut(s)	712, 1959, 2021, 2291
DpnII	GATC	4 cut(s)	710, 1957, 2019, 2289
DraI	TTTAAA	2 cut(s)	888, 1459
DraIII	CACNNNGTG	1 cut(s)	1612
DrdI	GACNNNNNNGTC	1 cut(s)	1993
DseDI	GACNNNNNNGTC	1 cut(s)	1993
EaeI	YGGCCR	2 cut(s)	646, 1048
Eam1104I	CTCTTC	4 cut(s)	70, 1065, 1269, 2250
EarI	CTCTTC	4 cut(s)	70, 1065, 1269, 2250
Ecl136II	GAGCTC	2 cut(s)	7, 546
Eco130I	CCWWGG	5 cut(s)	31, 1026, 1721, 1804, 2164
Eco24I	GRGCYC	2 cut(s)	9, 548
Eco47I	GGWCC	1 cut(s)	1981
Eco53kI	GAGCTC	2 cut(s)	7, 546
Eco57I	CTGAAG	7 cut(s)	624, 858, 1239, 1250, 1911, 2274, 2279
Eco72I	CACGTG	1 cut(s)	172
Eco88I	CYCGRG	1 cut(s)	131
EcoICRI	GAGCTC	2 cut(s)	7, 546
EcoO109I	RGGNCCY	1 cut(s)	1981
EcoRI	GAATTC	1 cut(s)	1406
EcoRII	CCWGG	1 cut(s)	1640
EcoT14I	CCWWGG	5 cut(s)	31, 1026, 1721, 1804, 2164
EcoT22I	ATGCAT	1 cut(s)	2310
EcoT38I	GRGCYC	2 cut(s)	9, 548
ErhI	CCWWGG	5 cut(s)	31, 1026, 1721, 1804, 2164
FalI	AAGNNNNNCTT	9 cut(s)	856, 888, 1047, 1079, 1674, 1706, 1884, 1916, 2451
FaqI	GGGAC	1 cut(s)	968
FauI	CCCGC	2 cut(s)	316, 2322
FblI	GTMKAC	3 cut(s)	253, 1653, 2160
Fnu4HI	GCNGC	7 cut(s)	756, 1353, 1820, 1967, 2237, 2240, 2325
FokI	GGATG	3 cut(s)	880, 1521, 2376
FriOI	GRGCYC	2 cut(s)	9, 548
Fsp4HI	GCNGC	7 cut(s)	756, 1353, 1820, 1967, 2237, 2240, 2325
FspBI	CTAG	9 cut(s)	405, 708, 819, 831, 935, 1181, 1758, 1785, 1835
FspI	TGCGCA	1 cut(s)	2075
GlaI	GCGC	2 cut(s)	983, 2075
GluI	GCNGC	7 cut(s)	756, 1353, 1820, 1967, 2237, 2240, 2325
GsaI	CCCAGC	1 cut(s)	1508
GsuI	CTGGAG	6 cut(s)	532, 564, 1368, 1942, 2034, 2145
HaeIII	GGCC	8 cut(s)	36, 100, 648, 1025, 1050, 1160, 2047, 2134
HapII	CCGG	5 cut(s)	38, 71, 97, 1715, 2040
HgaI	GACGC	2 cut(s)	1091, 1996
HhaI	GCGC	2 cut(s)	984, 2076
Hin1I	GRCGYC	2 cut(s)	1083, 1988
Hin6I	GCGC	2 cut(s)	982, 2074
HinP1I	GCGC	2 cut(s)	982, 2074
HincII	GTYRAC	2 cut(s)	478, 674
HindII	GTYRAC	2 cut(s)	478, 674
HindIII	AAGCTT	5 cut(s)	198, 374, 497, 1221, 2352
HinfI	GANTC	8 cut(s)	47, 878, 995, 1333, 1675, 1838, 2114, 2413
HpaII	CCGG	5 cut(s)	38, 71, 97, 1715, 2040
Hpy166II	GTNNAC	6 cut(s)	169, 254, 478, 674, 1654, 2161
Hpy8I	GTNNAC	6 cut(s)	169, 254, 478, 674, 1654, 2161
Hpy99I	CGWCG	1 cut(s)	87
HpyCH4III	ACNGT	5 cut(s)	223, 600, 1281, 1516, 1907
HpyCH4IV	ACGT	4 cut(s)	85, 171, 891, 2339
HpyF3I	CTNAG	9 cut(s)	117, 123, 295, 858, 1064, 1077, 1449, 1929, 2119
HpySE526I	ACGT	4 cut(s)	85, 171, 891, 2339
Hsp92I	GRCGYC	2 cut(s)	1083, 1988
HspAI	GCGC	2 cut(s)	982, 2074
Kzo9I	GATC	4 cut(s)	710, 1957, 2019, 2289
LguI	GCTCTTC	1 cut(s)	2250
LmnI	GCTCC	5 cut(s)	4, 509, 551, 583, 1349
Lsp1109I	GCAGC	6 cut(s)	742, 1806, 1953, 2248, 2251, 2336
LweI	GCATC	5 cut(s)	625, 767, 956, 1543, 1609
MaeI	CTAG	9 cut(s)	405, 708, 819, 831, 935, 1181, 1758, 1785, 1835
MaeII	ACGT	4 cut(s)	85, 171, 891, 2339
MaeIII	GTNAC	8 cut(s)	158, 334, 1604, 1612, 1885, 2035, 2367, 2414
MalI	GATC	4 cut(s)	712, 1959, 2021, 2291
MbiI	CCGCTC	2 cut(s)	280, 1324
MboI	GATC	4 cut(s)	710, 1957, 2019, 2289
MflI	RGATCY	1 cut(s)	1957
MhlI	GDGCHC	6 cut(s)	9, 171, 548, 580, 1195, 1765
MlsI	TGGCCA	1 cut(s)	648
MluNI	TGGCCA	1 cut(s)	648
MlyI	GAGTC	2 cut(s)	1684, 2422
MmeI	TCCRAC	2 cut(s)	106, 197
Mox20I	TGGCCA	1 cut(s)	648
Mph1103I	ATGCAT	1 cut(s)	2310
MroXI	GAANNNNTTC	1 cut(s)	1308
MscI	TGGCCA	1 cut(s)	648
MseI	TTAA	4 cut(s)	417, 887, 1059, 1458
MslI	CAYNNNNRTG	2 cut(s)	431, 533
Msp20I	TGGCCA	1 cut(s)	648
MspA1I	CMGCKG	3 cut(s)	1920, 2123, 2327
MspI	CCGG	5 cut(s)	38, 71, 97, 1715, 2040
MspR9I	CCNGG	2 cut(s)	38, 1642
Mva1269I	GAATGC	1 cut(s)	1015
MvaI	CCWGG	1 cut(s)	1642
MvnI	CGCG	2 cut(s)	2152, 2382
NciI	CCSGG	1 cut(s)	38
NcoI	CCATGG	3 cut(s)	31, 1721, 1804
NdeII	GATC	4 cut(s)	710, 1957, 2019, 2289
NlaIV	GGNNCC	3 cut(s)	1351, 1719, 1982
NmeAIII	GCCGAG	4 cut(s)	967, 1076, 1214, 1585
NmuCI	GTSAC	7 cut(s)	158, 334, 1604, 1612, 1885, 2035, 2414
NruI	TCGCGA	1 cut(s)	2382
NsbI	TGCGCA	1 cut(s)	2075
NsiI	ATGCAT	1 cut(s)	2310
NspI	RCATGY	4 cut(s)	623, 792, 1440, 2249
PaqCI	CACCTGC	1 cut(s)	697
PciI	ACATGT	1 cut(s)	619
PciSI	GCTCTTC	1 cut(s)	2250
PctI	GAATGC	1 cut(s)	1015
PdmI	GAANNNNTTC	1 cut(s)	1308
PfeI	GAWTC	6 cut(s)	47, 878, 995, 1333, 1838, 2114
Pfl23II	CGTACG	1 cut(s)	2340
PkrI	GCNGC	7 cut(s)	757, 1354, 1821, 1968, 2238, 2241, 2326
PleI	GAGTC	2 cut(s)	1683, 2421
PmaCI	CACGTG	1 cut(s)	172
PmlI	CACGTG	1 cut(s)	172
PpsI	GAGTC	2 cut(s)	1683, 2421
Ppu21I	YACGTR	2 cut(s)	172, 2340
PpuMI	RGGWCCY	1 cut(s)	1981
PscI	ACATGT	1 cut(s)	619
PsiI	TTATAA	1 cut(s)	1491
Psp124BI	GAGCTC	2 cut(s)	9, 548
Psp1406I	AACGTT	1 cut(s)	891
Psp5II	RGGWCCY	1 cut(s)	1981
Psp6I	CCWGG	1 cut(s)	1640
PspCI	CACGTG	1 cut(s)	172
PspFI	CCCAGC	1 cut(s)	1504
PspGI	CCWGG	1 cut(s)	1640
PspLI	CGTACG	1 cut(s)	2340
PspN4I	GGNNCC	3 cut(s)	1351, 1719, 1982
PspPI	GGNCC	3 cut(s)	35, 1158, 1981
PspPPI	RGGWCCY	1 cut(s)	1981
PstI	CTGCAG	4 cut(s)	681, 694, 1971, 2031
PstNI	CAGNNNCTG	2 cut(s)	881, 1004
PsuI	RGATCY	1 cut(s)	1957
PvuII	CAGCTG	2 cut(s)	2123, 2327
RruI	TCGCGA	1 cut(s)	2382
RsaI	GTAC	4 cut(s)	516, 823, 1578, 2342
RsaNI	GTAC	4 cut(s)	515, 822, 1577, 2341
RseI	CAYNNNNRTG	2 cut(s)	431, 533
SacI	GAGCTC	2 cut(s)	9, 548
SapI	GCTCTTC	1 cut(s)	2250
SaqAI	TTAA	4 cut(s)	417, 887, 1059, 1458
SatI	GCNGC	7 cut(s)	756, 1353, 1820, 1967, 2237, 2240, 2325
Sau3AI	GATC	4 cut(s)	710, 1957, 2019, 2289
Sau96I	GGNCC	3 cut(s)	35, 1158, 1981
SchI	GAGTC	2 cut(s)	1684, 2422
ScrFI	CCNGG	2 cut(s)	38, 1642
SduI	GDGCHC	6 cut(s)	9, 171, 548, 580, 1195, 1765
SfaNI	GCATC	5 cut(s)	625, 767, 956, 1543, 1609
SfcI	CTRYAG	4 cut(s)	677, 690, 1967, 2027
SinI	GGWCC	1 cut(s)	1981
SmiMI	CAYNNNNRTG	2 cut(s)	431, 533
SmlI	CTYRAG	1 cut(s)	1087
SmoI	CTYRAG	1 cut(s)	1087
SpeI	ACTAGT	1 cut(s)	818
SsiI	CCGC	9 cut(s)	26, 176, 278, 309, 1324, 1353, 1538, 1918, 2315
SspI	AATATT	3 cut(s)	805, 1171, 1708
SspMI	CTAG	9 cut(s)	405, 708, 819, 831, 935, 1181, 1758, 1785, 1835
SstI	GAGCTC	2 cut(s)	9, 548
StyD4I	CCNGG	2 cut(s)	36, 1640
StyI	CCWWGG	5 cut(s)	31, 1026, 1721, 1804, 2164
TaaI	ACNGT	5 cut(s)	223, 600, 1281, 1516, 1907
TaiI	ACGT	4 cut(s)	88, 174, 894, 2342
TaqI	TCGA	5 cut(s)	1207, 1253, 1339, 1909, 2105
TatI	WGTACW	1 cut(s)	821
TauI	GCSGC	1 cut(s)	1355
TfiI	GAWTC	6 cut(s)	47, 878, 995, 1333, 1838, 2114
Tru1I	TTAA	4 cut(s)	417, 887, 1059, 1458
Tru9I	TTAA	4 cut(s)	417, 887, 1059, 1458
TscAI	CASTG	4 cut(s)	588, 1286, 1894, 2271
TseFI	GTSAC	7 cut(s)	158, 334, 1604, 1612, 1885, 2035, 2414
TseI	GCWGC	6 cut(s)	755, 1819, 1966, 2236, 2239, 2324
Tsp45I	GTSAC	7 cut(s)	158, 334, 1604, 1612, 1885, 2035, 2414
TspDTI	ATGAA	9 cut(s)	248, 254, 258, 386, 443, 462, 545, 747, 797
TspGWI	ACGGA	3 cut(s)	379, 938, 1753
TspRI	CASTG	4 cut(s)	588, 1286, 1894, 2271
VneI	GTGCAC	1 cut(s)	167
VpaK11BI	GGWCC	1 cut(s)	1981
XapI	RAATTY	7 cut(s)	246, 450, 696, 826, 1242, 1406, 2095
XbaI	TCTAGA	4 cut(s)	404, 707, 1180, 1784
XceI	RCATGY	4 cut(s)	623, 792, 1440, 2249
XmiI	GTMKAC	3 cut(s)	253, 1653, 2160
XmnI	GAANNNNTTC	1 cut(s)	1308
XspI	CTAG	9 cut(s)	405, 708, 819, 831, 935, 1181, 1758, 1785, 1835
Zsp2I	ATGCAT	1 cut(s)	2310

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.