MD03G1121000.v1.1

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014999
Physical Location & Seq
Reverse (-)
11254583 .. 11259866
5284 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1121000.v1.1.491

Sequence Viewer

Length: 2178 bp
ATGGGAAGCAAATGGAGGAAAGCCAAGCTTGCTTTTGGGTTGAATTCTTGTCTCTATGTTCCTCAAACCAGAGAGGAACCGCCAGTCTCATCGAATGACGTTGCCGCATCTAGATTCTCCGACGCCGTTTCATCCTCCTCTCTGCTTTCTCCGATGGGTGGTGGCTCCGATTGCCGACCCACCACGCCCACGCCGTCGTCTTCCGGCCTCCGGTTGCCCAAAACTGGAACCAAGTGCTCCAAGAGGACCTGCGCAATATGCTTGACGACCATGAAGACAGGACAGGGGCATGCCATTTTCACTGCAGAGTGCTCCCACGCTTTCCACTTCCACTGTATTACTTCTAATGTAAAACATGGAAACCAAATTTGTCCAGTTTGCAGAGCAAAGTGGAAAGAAATCCCCTTTCAGAGCCCCGCTTCTGATCTTTCAGGCAGTGGTTCTAGAATTAACCCTGTTGGTTGGCCCCAGGATGATTCATGGATGGCCGTTTTACGACAAATTCCTCCCCCACGAGTTGATGCAACCCGACCGGTTTCTTCACTCTTTCACACTCCCGAACCAGTTATATTTGACGATGATGAAAGCTTAGATCAGCAACCTGAGATTTCCAACAAAAGCCCATCTGTCGAAGATGCTTATGATAACAATTCTATTCGAACAATAGAGGTCAAAACATATCCTGAAGTTCCGGCCATTCAAAGAGCAGCCTCTCATGATAACTTCACTGTTCTAATCCACCTTAAGGCTCCTCATACAAGTGGAAGACAGAATAGCAGCAGAAACCAGACACCGCCAGTATCTCAAATTTCTCGTGCTCCAGTAGATCTTGTGACAGTGCTTGATATCAGTGGCAGCATGGCAGGTACAAAACTTGCTTTGCTGAAACGGGCAATGGGGTTTGTTGTACAGAACCTTGGTCCATCTGATCGGCTGTCTGTAATTGCCTTCTCATCTACTGCCCGCCGGCTTTTTCCCCTTCGTCGGATGACAGATACTGGGCGACAGCAGGCATTGCAGGCGGTAAACTCTCTGGTTTCAAGTGGTGGGACAAACATTGCTGAGGCTCTTAGGAAAGGTACCAAGTTGTTAGTAGACCGCAAGTGGAAGAACCCAGTTTGCAGTATCATACTACTATCTGATGGGCAGGATACATACACTGTCAGTAGTCCTAGTGGGATCCATCCCCGATCAGATTACCAATCACTTATCCCAATCTCTATCCGTCGCAATAATGCAGCGGGCTTGCGAATTCCAGTTCACGCATTTGGATTTGGTGCAGACCATGATGCTGCCTCGATGCATTCAATCTCGGAAATTTCTGGTGGAACATTTTCTTTCATAGAAGCTGAGAGTGTGATTCAGGATGCATTTGCACAGTGTATTGGCGGCCTTTTGAGTGTGGTAGTGCAAGAACTAAAGGTTAGAATTGAGTGTGTTCACACGAGTTTGCAACTCGGATCAATAAAAGCAGGAAGTTACAGAACCAGCATGATGGCTGATGCAAGAATGGGTTGTATTGATGTTGGAGACCTGTATGCTGAAGAAGAAAGGGATTTTTTGGTGACAATCAATATTCCAGATGATGTGTCCGGTAATGACATGTCGCTGGTAAAGGTTAGCTGTGCTTACAGAGATCCCATTACGAAAGAAATGGTGAATTTGGAAGAAAGCAGTGAAGTCACGATCCAAAGGCCTGAAGTAGTAGGACAACTAATAGTGTCGCTGGAAGTAGACAGGCAGCAGAACAGGCTTCGTGCAGCAGAAGCAATGGCTGAGGCTAGAGTGGCAGCTGAGAACGGTGATTTGGCTGGTGCAGTCTCTGTCCTGGAAAGCTGTCGTATGGCATTGTCTGAAACTGCTTCCGCACTAGCTGGTGACTGCCTGTGTGTCTCACTTTCCGCTGAGTTAAAGGAGATGCAAGAAAGGATGGGAAACCGTGGTGCATATGAGGAATCGGGAAGAGCATACGTTTTATCAGGATTGAGCTCACACTCATGGCAGAGGGCAACTGCTCGAGGTGATTCCACAGATAGCACTAGCCTTGTGCTATCTTACCAAACTCCATCGATGATAGACATGGTGACACGTTCACAAACCATGTTGCTAGGGAACCCATCTCCCCAACGAGCCCTCAGCAGTGCTCAATCATTTCCGGCCAAGCCACAGCCACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

726

Amino Acids

78.29

Weight (kDa)

6.94

Isoelectric Point (pI)

51.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 79 - 128 1.7e-06 RING-H2 zinc finger domain
zf-RING_2 PF13639 83 - 128 5.1e-08 Ring finger domain
zf-RING_11 PF17123 84 - 113 3e-08 RING-like zinc finger
zf-C3HC4 PF00097 84 - 127 8.5e-07 Zinc finger, C3HC4 type (RING finger)
VWA_CoxE PF05762 271 - 388 2e-08 VWA domain containing CoxE-like protein
VWA PF00092 276 - 457 1.6e-22 von Willebrand factor type A domain
VWA_3 PF13768 276 - 447 6.3e-18 von Willebrand factor type A domain
VWA_2 PF13519 277 - 383 1.3e-18 von Willebrand factor type A domain
WAV3_C PF25243 586 - 645 1.9e-06 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint PF14624 629 - 700 1.4e-19 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014999)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60710
fragaria_vesca FvH4_3g31730 FvH4_3g31730
malus_domestica MD03G1121000.v1.1 MD11G1139800.v1.1
pyrus_communis pycom11g11540
rosa_chinensis RchiOBHm_Chr5g0058461
rosa_laevigata RLG00000035222
rosa_multiflora Rmu_sc0000367.1_g000122
rosa_roxburghii Rroxscaffold_1G00022080
rosa_rugosa Rorug05G0314900
rosa_samantha Rh5AG381400 Rh5BG392900 Rh5CG417100 Rh5DG408100
rosa_wichuraiana Rw5G035930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 253
Acc36I ACCTGC 2 cut(s) 257, 854
Acc65I GGTACC 1 cut(s) 1079
AccB1I GGYRCC 1 cut(s) 1079
AccI GTMKAC 2 cut(s) 1095, 1734
AclWI GGATC 5 cut(s) 1174, 1187, 1468, 1631, 1681
AcoI YGGCCR 3 cut(s) 486, 693, 2157
AcsI RAATTY 7 cut(s) 43, 366, 501, 807, 1251, 1317, 1660
AcuI CTGAAG 3 cut(s) 705, 1563, 1719
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 3 cut(s) 868, 909, 1081
AfiI CCNNNNNNNGG 5 cut(s) 158, 210, 224, 745, 984
AflII CTTAAG 1 cut(s) 743
AflIII ACRYGT 2 cut(s) 1602, 2087
AgeI ACCGGT 1 cut(s) 532
AgsI TTSAA 4 cut(s) 43, 701, 1041, 1308
AjnI CCWGG 2 cut(s) 468, 1827
AjuI GAANNNNNNNTTGG 2 cut(s) 1790, 1822
AluBI AGCT 8 cut(s) 28, 588, 1349, 1623, 1793, 1836, 1874, 1989
AluI AGCT 8 cut(s) 28, 588, 1349, 1623, 1793, 1836, 1874, 1989
Alw21I GWGCWC 5 cut(s) 239, 314, 820, 1991, 2146
Alw26I GTCTC 5 cut(s) 56, 91, 1524, 1825, 1897
AlwI GGATC 5 cut(s) 1174, 1187, 1468, 1631, 1681
AlwNI CAGNNNCTG 2 cut(s) 998, 1823
Ama87I CYCGRG 1 cut(s) 2016
AoxI GGCC 7 cut(s) 205, 464, 486, 693, 1390, 1694, 2157
ApeKI GCWGC 8 cut(s) 707, 777, 855, 1238, 1292, 1741, 1760, 1790
ApoI RAATTY 7 cut(s) 43, 366, 501, 807, 1251, 1317, 1660
AsiGI ACCGGT 1 cut(s) 532
Asp700I GAANNNNTTC 1 cut(s) 1333
Asp718I GGTACC 1 cut(s) 1079
AspLEI GCGC 1 cut(s) 254
AspS9I GGNCC 3 cut(s) 246, 465, 920
AsuHPI GGTGA 6 cut(s) 1576, 1669, 1814, 1889, 2033, 2095
AsuII TTCGAA 1 cut(s) 658
AvaI CYCGRG 1 cut(s) 2016
AvaII GGWCC 2 cut(s) 246, 920
BamHI GGATCC 1 cut(s) 1179
BanI GGYRCC 1 cut(s) 1079
BanII GRGCYC 3 cut(s) 416, 1991, 2134
BauI CACGAG 3 cut(s) 513, 813, 1444
BbsI GAAGAC 3 cut(s) 192, 281, 772
Bbv12I GWGCWC 5 cut(s) 239, 314, 820, 1991, 2146
BbvCI CCTCAGC 3 cut(s) 1062, 1776, 2135
BbvI GCAGC 8 cut(s) 719, 789, 867, 1250, 1279, 1753, 1772, 1802
BceAI ACGGC 3 cut(s) 110, 178, 473
BciT130I CCWGG 2 cut(s) 470, 1829
BciVI GTATCC 1 cut(s) 1144
BcoDI GTCTC 5 cut(s) 56, 91, 1524, 1825, 1897
BfaI CTAG 7 cut(s) 111, 444, 1173, 1782, 1871, 2040, 2108
BfmI CTRYAG 1 cut(s) 303
BfrI CTTAAG 1 cut(s) 743
BfuAI ACCTGC 2 cut(s) 257, 854
BfuI GTATCC 1 cut(s) 1144
BglII AGATCT 1 cut(s) 826
Bme1390I CCNGG 2 cut(s) 470, 1829
Bme18I GGWCC 2 cut(s) 246, 920
BmeT110I CYCGRG 1 cut(s) 2016
BmgT120I GGNCC 3 cut(s) 246, 465, 920
BmiI GGNNCC 8 cut(s) 78, 166, 229, 467, 750, 1081, 1181, 2114
BmrFI CCNGG 2 cut(s) 470, 1829
BmrI ACTGGG 2 cut(s) 1008, 1109
BmsI GCATC 8 cut(s) 116, 511, 625, 1279, 1290, 1357, 1492, 1908
BmuI ACTGGG 2 cut(s) 1008, 1109
BpiI GAAGAC 3 cut(s) 192, 281, 772
BpmI CTGGAG 1 cut(s) 804
Bpu10I CCTNAGC 3 cut(s) 1062, 1776, 2135
Bpu14I TTCGAA 1 cut(s) 658
Bsa29I ATCGAT 1 cut(s) 2069
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 1524
BsaJI CCNNGG 3 cut(s) 468, 916, 1939
BsaWI WCCGGW 3 cut(s) 210, 532, 1592
Bsc4I CCNNNNNNNGG 5 cut(s) 158, 210, 224, 745, 984
Bse118I RCCGGY 2 cut(s) 532, 966
Bse1I ACTGG 9 cut(s) 83, 229, 374, 563, 797, 821, 1003, 1115, 1256
Bse3DI GCAATG 4 cut(s) 900, 1013, 1056, 1776
BseBI CCWGG 2 cut(s) 470, 1829
BseCI ATCGAT 1 cut(s) 2069
BseDI CCNNGG 3 cut(s) 468, 916, 1939
BseGI GGATG 7 cut(s) 131, 478, 489, 993, 1183, 1372, 1935
BseLI CCNNNNNNNGG 5 cut(s) 158, 210, 224, 745, 984
BseMI GCAATG 4 cut(s) 900, 1013, 1056, 1776
BseMII CTCAG 7 cut(s) 594, 1053, 1341, 1767, 1785, 1896, 2149
BseNI ACTGG 9 cut(s) 83, 229, 374, 563, 797, 821, 1003, 1115, 1256
BseRI GAGGAG 2 cut(s) 127, 741
BseXI GCAGC 8 cut(s) 719, 789, 867, 1250, 1279, 1753, 1772, 1802
BsgI GTGCAG 3 cut(s) 1299, 1779, 1836
Bsh1285I CGRYCG 1 cut(s) 533
BshFI GGCC 7 cut(s) 207, 466, 488, 695, 1392, 1696, 2159
BshNI GGYRCC 1 cut(s) 1079
BshTI ACCGGT 1 cut(s) 532
BshVI ATCGAT 1 cut(s) 2069
BsiEI CGRYCG 1 cut(s) 533
BsiHKAI GWGCWC 5 cut(s) 239, 314, 820, 1991, 2146
BsiHKCI CYCGRG 1 cut(s) 2016
BsiSI CCGG 7 cut(s) 204, 211, 533, 692, 967, 1593, 2156
BslFI GGGAC 1 cut(s) 1063
BslI CCNNNNNNNGG 5 cut(s) 158, 210, 224, 745, 984
BsmAI GTCTC 5 cut(s) 56, 91, 1524, 1825, 1897
BsmFI GGGAC 1 cut(s) 1063
BsmI GAATGC 1 cut(s) 1303
BsnI GGCC 7 cut(s) 207, 466, 488, 695, 1392, 1696, 2159
Bso31I GGTCTC 1 cut(s) 1524
BsoBI CYCGRG 1 cut(s) 2016
Bsp119I TTCGAA 1 cut(s) 658
Bsp1286I GDGCHC 7 cut(s) 239, 314, 416, 820, 1991, 2134, 2146
Bsp1407I TGTACA 1 cut(s) 907
Bsp143I GATC 9 cut(s) 424, 592, 826, 928, 1179, 1190, 1460, 1636, 1686
BspANI GGCC 7 cut(s) 207, 466, 488, 695, 1392, 1696, 2159
BspCNI CTCAG 7 cut(s) 595, 1054, 1342, 1768, 1786, 1897, 2148
BspDI ATCGAT 1 cut(s) 2069
BspHI TCATGA 1 cut(s) 715
BspLI GGNNCC 8 cut(s) 78, 166, 229, 467, 750, 1081, 1181, 2114
BspMAI CTGCAG 1 cut(s) 307
BspMI ACCTGC 2 cut(s) 257, 854
BspPI GGATC 5 cut(s) 1174, 1187, 1468, 1631, 1681
BspQI GCTCTTC 1 cut(s) 1957
BspT104I TTCGAA 1 cut(s) 658
BspT107I GGYRCC 1 cut(s) 1079
BspTI CTTAAG 1 cut(s) 743
BspTNI GGTCTC 1 cut(s) 1524
BsrDI GCAATG 4 cut(s) 900, 1013, 1056, 1776
BsrFI RCCGGY 2 cut(s) 532, 966
BsrGI TGTACA 1 cut(s) 907
BsrI ACTGG 9 cut(s) 83, 229, 374, 563, 797, 821, 1003, 1115, 1256
BssAI RCCGGY 2 cut(s) 532, 966
BssECI CCNNGG 3 cut(s) 468, 916, 1939
BssMI GATC 9 cut(s) 424, 592, 826, 928, 1179, 1190, 1460, 1636, 1686
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 3 cut(s) 513, 813, 1444
BssT1I CCWWGG 1 cut(s) 916
Bst2BI CACGAG 3 cut(s) 513, 813, 1444
Bst2UI CCWGG 2 cut(s) 470, 1829
Bst4CI ACNGT 7 cut(s) 335, 730, 838, 1162, 1380, 1802, 1940
Bst6I CTCTTC 1 cut(s) 1957
BstACI GRCGYC 1 cut(s) 123
BstAFI CTTAAG 1 cut(s) 743
BstAPI GCANNNNNTGC 1 cut(s) 1015
BstAUI TGTACA 1 cut(s) 907
BstBI TTCGAA 1 cut(s) 658
BstC8I GCNNGC 8 cut(s) 30, 291, 964, 968, 1011, 1020, 1243, 1247
BstDEI CTNAG 9 cut(s) 589, 603, 1062, 1070, 1350, 1776, 1794, 1905, 2135
BstDSI CCRYGG 1 cut(s) 1939
BstF5I GGATG 7 cut(s) 131, 478, 489, 993, 1183, 1372, 1935
BstHHI GCGC 1 cut(s) 254
BstKTI GATC 9 cut(s) 427, 595, 829, 931, 1182, 1193, 1463, 1639, 1689
BstMAI GTCTC 5 cut(s) 56, 91, 1524, 1825, 1897
BstMBI GATC 9 cut(s) 424, 592, 826, 928, 1179, 1190, 1460, 1636, 1686
BstMCI CGRYCG 1 cut(s) 533
BstMWI GCNNNNNNNGC 8 cut(s) 29, 171, 258, 1015, 1019, 1750, 1766, 1787
BstNI CCWGG 2 cut(s) 470, 1829
BstNSI RCATGY 2 cut(s) 293, 1606
BstSCI CCNGG 2 cut(s) 468, 1827
BstSFI CTRYAG 1 cut(s) 303
BstV1I GCAGC 8 cut(s) 719, 789, 867, 1250, 1279, 1753, 1772, 1802
BstV2I GAAGAC 3 cut(s) 192, 281, 772
BstX2I RGATCY 3 cut(s) 826, 1179, 1636
BstXI CCANNNNNNTGG 1 cut(s) 1495
BstYI RGATCY 3 cut(s) 826, 1179, 1636
Bsu15I ATCGAT 1 cut(s) 2069
BsuI GTATCC 1 cut(s) 1144
BsuRI GGCC 7 cut(s) 207, 466, 488, 695, 1392, 1696, 2159
BsuTUI ATCGAT 1 cut(s) 2069
BtgI CCRYGG 1 cut(s) 1939
BtsCI GGATG 7 cut(s) 131, 478, 489, 993, 1183, 1372, 1935
BtsI GCAGTG 4 cut(s) 300, 442, 1681, 2146
BveI ACCTGC 2 cut(s) 257, 854
Cac8I GCNNGC 8 cut(s) 30, 291, 964, 968, 1011, 1020, 1243, 1247
CaiI CAGNNNCTG 2 cut(s) 998, 1823
CciI TCATGA 1 cut(s) 715
CfoI GCGC 1 cut(s) 254
Cfr10I RCCGGY 2 cut(s) 532, 966
Cfr13I GGNCC 3 cut(s) 246, 465, 920
ClaI ATCGAT 1 cut(s) 2069
CseI GACGC 1 cut(s) 131
Csp6I GTAC 3 cut(s) 867, 908, 1080
CspAI ACCGGT 1 cut(s) 532
CviQI GTAC 3 cut(s) 867, 908, 1080
DdeI CTNAG 9 cut(s) 589, 603, 1062, 1070, 1350, 1776, 1794, 1905, 2135
DpnI GATC 9 cut(s) 426, 594, 828, 930, 1181, 1192, 1462, 1638, 1688
DpnII GATC 9 cut(s) 424, 592, 826, 928, 1179, 1190, 1460, 1636, 1686
EaeI YGGCCR 3 cut(s) 486, 693, 2157
Eam1104I CTCTTC 1 cut(s) 1957
EarI CTCTTC 1 cut(s) 1957
Ecl136II GAGCTC 1 cut(s) 1989
Eco130I CCWWGG 1 cut(s) 916
Eco147I AGGCCT 1 cut(s) 1696
Eco24I GRGCYC 3 cut(s) 416, 1991, 2134
Eco31I GGTCTC 1 cut(s) 1524
Eco32I GATATC 1 cut(s) 847
Eco47I GGWCC 2 cut(s) 246, 920
Eco53kI GAGCTC 1 cut(s) 1989
Eco57I CTGAAG 3 cut(s) 705, 1563, 1719
Eco88I CYCGRG 1 cut(s) 2016
EcoICRI GAGCTC 1 cut(s) 1989
EcoO109I RGGNCCY 1 cut(s) 246
EcoRI GAATTC 2 cut(s) 43, 1251
EcoRII CCWGG 2 cut(s) 468, 1827
EcoRV GATATC 1 cut(s) 847
EcoT14I CCWWGG 1 cut(s) 916
EcoT22I ATGCAT 2 cut(s) 1305, 1372
EcoT38I GRGCYC 3 cut(s) 416, 1991, 2134
ErhI CCWWGG 1 cut(s) 916
FalI AAGNNNNNCTT 2 cut(s) 12, 44
FaqI GGGAC 1 cut(s) 1063
FauI CCCGC 3 cut(s) 424, 971, 1234
FauNDI CATATG 1 cut(s) 1948
FblI GTMKAC 2 cut(s) 1095, 1734
FokI GGATG 7 cut(s) 118, 485, 496, 1000, 1170, 1379, 1942
FriOI GRGCYC 3 cut(s) 416, 1991, 2134
FspBI CTAG 7 cut(s) 111, 444, 1173, 1782, 1871, 2040, 2108
FspI TGCGCA 1 cut(s) 253
GlaI GCGC 1 cut(s) 253
GsuI CTGGAG 1 cut(s) 804
HaeIII GGCC 7 cut(s) 207, 466, 488, 695, 1392, 1696, 2159
HapII CCGG 7 cut(s) 204, 211, 533, 692, 967, 1593, 2156
HgaI GACGC 1 cut(s) 131
HhaI GCGC 1 cut(s) 254
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 1 cut(s) 252
HinP1I GCGC 1 cut(s) 252
HindIII AAGCTT 2 cut(s) 26, 586
HinfI GANTC 5 cut(s) 114, 476, 1360, 1955, 2024
HpaII CCGG 7 cut(s) 204, 211, 533, 692, 967, 1593, 2156
HphI GGTGA 6 cut(s) 1576, 1669, 1814, 1889, 2033, 2095
Hpy166II GTNNAC 6 cut(s) 1027, 1096, 1261, 1441, 1735, 2093
Hpy8I GTNNAC 6 cut(s) 1027, 1096, 1261, 1441, 1735, 2093
Hpy99I CGWCG 4 cut(s) 125, 199, 987, 1230
HpyAV CCTTC 2 cut(s) 958, 989
HpyCH4III ACNGT 7 cut(s) 335, 730, 838, 1162, 1380, 1802, 1940
HpyCH4IV ACGT 3 cut(s) 99, 1971, 2089
HpyF10VI GCNNNNNNNGC 8 cut(s) 29, 171, 258, 1015, 1019, 1750, 1766, 1787
HpyF3I CTNAG 9 cut(s) 589, 603, 1062, 1070, 1350, 1776, 1794, 1905, 2135
HpySE526I ACGT 3 cut(s) 99, 1971, 2089
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 1 cut(s) 252
KpnI GGTACC 1 cut(s) 1083
KroI GCCGGC 1 cut(s) 966
KroNI GCCGGC 1 cut(s) 968
Kzo9I GATC 9 cut(s) 424, 592, 826, 928, 1179, 1190, 1460, 1636, 1686
LguI GCTCTTC 1 cut(s) 1957
LmnI GCTCC 5 cut(s) 170, 242, 317, 754, 823
Lsp1109I GCAGC 8 cut(s) 719, 789, 867, 1250, 1279, 1753, 1772, 1802
LweI GCATC 8 cut(s) 116, 511, 625, 1279, 1290, 1357, 1492, 1908
MaeI CTAG 7 cut(s) 111, 444, 1173, 1782, 1871, 2040, 2108
MaeII ACGT 3 cut(s) 99, 1971, 2089
MaeIII GTNAC 6 cut(s) 832, 1478, 1564, 1681, 1877, 2083
MalI GATC 9 cut(s) 426, 594, 828, 930, 1181, 1192, 1462, 1638, 1688
MboI GATC 9 cut(s) 424, 592, 826, 928, 1179, 1190, 1460, 1636, 1686
MflI RGATCY 3 cut(s) 826, 1179, 1636
MhlI GDGCHC 7 cut(s) 239, 314, 416, 820, 1991, 2134, 2146
MmeI TCCRAC 4 cut(s) 144, 636, 965, 1507
Mph1103I ATGCAT 2 cut(s) 1305, 1372
MroNI GCCGGC 1 cut(s) 966
MroXI GAANNNNTTC 1 cut(s) 1333
MseI TTAA 3 cut(s) 450, 744, 1910
MslI CAYNNNNRTG 2 cut(s) 759, 1996
MspA1I CMGCKG 3 cut(s) 1241, 1793, 1904
MspCI CTTAAG 1 cut(s) 743
MspI CCGG 7 cut(s) 204, 211, 533, 692, 967, 1593, 2156
MspR9I CCNGG 2 cut(s) 470, 1829
Mva1269I GAATGC 1 cut(s) 1303
MvaI CCWGG 2 cut(s) 470, 1829
MwoI GCNNNNNNNGC 8 cut(s) 29, 171, 258, 1015, 1019, 1750, 1766, 1787
NaeI GCCGGC 1 cut(s) 968
NdeI CATATG 1 cut(s) 1948
NdeII GATC 9 cut(s) 424, 592, 826, 928, 1179, 1190, 1460, 1636, 1686
NgoMIV GCCGGC 1 cut(s) 966
NlaIV GGNNCC 8 cut(s) 78, 166, 229, 467, 750, 1081, 1181, 2114
NmuCI GTSAC 5 cut(s) 832, 1564, 1681, 1877, 2083
NsbI TGCGCA 1 cut(s) 253
NsiI ATGCAT 2 cut(s) 1305, 1372
NspI RCATGY 2 cut(s) 293, 1606
NspV TTCGAA 1 cut(s) 658
PaeI GCATGC 1 cut(s) 293
PaeR7I CTCGAG 1 cut(s) 2016
PagI TCATGA 1 cut(s) 715
PceI AGGCCT 1 cut(s) 1696
PciI ACATGT 1 cut(s) 1602
PciSI GCTCTTC 1 cut(s) 1957
PcsI WCGNNNNNNNCGW 1 cut(s) 191
PctI GAATGC 1 cut(s) 1303
PdiI GCCGGC 1 cut(s) 968
PdmI GAANNNNTTC 1 cut(s) 1333
PfeI GAWTC 5 cut(s) 114, 476, 1360, 1955, 2024
PfoI TCCNGGA 1 cut(s) 1827
PinAI ACCGGT 1 cut(s) 532
PpuMI RGGWCCY 1 cut(s) 246
PscI ACATGT 1 cut(s) 1602
Psp124BI GAGCTC 1 cut(s) 1991
Psp5II RGGWCCY 1 cut(s) 246
Psp6I CCWGG 2 cut(s) 468, 1827
PspGI CCWGG 2 cut(s) 468, 1827
PspN4I GGNNCC 8 cut(s) 78, 166, 229, 467, 750, 1081, 1181, 2114
PspPI GGNCC 3 cut(s) 246, 465, 920
PspPPI RGGWCCY 1 cut(s) 246
PspXI VCTCGAGB 1 cut(s) 2016
PstI CTGCAG 1 cut(s) 307
PstNI CAGNNNCTG 2 cut(s) 998, 1823
PsuI RGATCY 3 cut(s) 826, 1179, 1636
PvuII CAGCTG 1 cut(s) 1793
RsaI GTAC 3 cut(s) 868, 909, 1081
RsaNI GTAC 3 cut(s) 867, 908, 1080
RseI CAYNNNNRTG 2 cut(s) 759, 1996
SacI GAGCTC 1 cut(s) 1991
SapI GCTCTTC 1 cut(s) 1957
SaqAI TTAA 3 cut(s) 450, 744, 1910
Sau3AI GATC 9 cut(s) 424, 592, 826, 928, 1179, 1190, 1460, 1636, 1686
Sau96I GGNCC 3 cut(s) 246, 465, 920
ScrFI CCNGG 2 cut(s) 470, 1829
SduI GDGCHC 7 cut(s) 239, 314, 416, 820, 1991, 2134, 2146
SfaNI GCATC 8 cut(s) 116, 511, 625, 1279, 1290, 1357, 1492, 1908
SfcI CTRYAG 1 cut(s) 303
Sfr274I CTCGAG 1 cut(s) 2016
SfuI TTCGAA 1 cut(s) 658
SinI GGWCC 2 cut(s) 246, 920
SlaI CTCGAG 1 cut(s) 2016
SmiMI CAYNNNNRTG 2 cut(s) 759, 1996
SmlI CTYRAG 2 cut(s) 743, 2016
SmoI CTYRAG 2 cut(s) 743, 2016
SphI GCATGC 1 cut(s) 293
SseBI AGGCCT 1 cut(s) 1696
SspI AATATT 1 cut(s) 1576
SspMI CTAG 7 cut(s) 111, 444, 1173, 1782, 1871, 2040, 2108
SstI GAGCTC 1 cut(s) 1991
StuI AGGCCT 1 cut(s) 1696
StyD4I CCNGG 2 cut(s) 468, 1827
StyI CCWWGG 1 cut(s) 916
TaaI ACNGT 7 cut(s) 335, 730, 838, 1162, 1380, 1802, 1940
TaiI ACGT 3 cut(s) 102, 1974, 2092
TaqI TCGA 6 cut(s) 92, 630, 658, 1298, 2017, 2069
TatI WGTACW 1 cut(s) 907
TauI GCSGC 2 cut(s) 107, 1392
TfiI GAWTC 5 cut(s) 114, 476, 1360, 1955, 2024
Tru1I TTAA 3 cut(s) 450, 744, 1910
Tru9I TTAA 3 cut(s) 450, 744, 1910
TseFI GTSAC 5 cut(s) 832, 1564, 1681, 1877, 2083
TseI GCWGC 8 cut(s) 707, 777, 855, 1238, 1292, 1741, 1760, 1790
Tsp45I GTSAC 5 cut(s) 832, 1564, 1681, 1877, 2083
TspDTI ATGAA 5 cut(s) 120, 287, 468, 597, 1330
TspGWI ACGGA 1 cut(s) 1214
Vha464I CTTAAG 1 cut(s) 743
VpaK11BI GGWCC 2 cut(s) 246, 920
XapI RAATTY 7 cut(s) 43, 366, 501, 807, 1251, 1317, 1660
XbaI TCTAGA 2 cut(s) 110, 443
XceI RCATGY 2 cut(s) 293, 1606
XhoI CTCGAG 1 cut(s) 2016
XmiI GTMKAC 2 cut(s) 1095, 1734
XmnI GAANNNNTTC 1 cut(s) 1333
XspI CTAG 7 cut(s) 111, 444, 1173, 1782, 1871, 2040, 2108
Zsp2I ATGCAT 2 cut(s) 1305, 1372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.