Rroxscaffold_1G00022080

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014999
Physical Location & Seq
Reverse (-)
27159104 .. 27163123
4020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00022080.1

Sequence Viewer

Length: 2076 bp
ATGGGGAGCAAATGGAGGAAAGCCAAGCTTGCTCTTGGCTTGAACACTTGTCTTTATGTTCCTCAAACTGCAGAGGAATCATCTCCACCGTCTCCAAACGGCGTCGTCGAGTCTAGACACTCCGACGCCGTTTCTTCCTCCTCTCTGCTTTCTCCGACTGGTATTGGCGCCGACCGGCGACCCACCACGCCCACTCCGTCCTCCTCGGGGCTCCGGTTGCCCAAAAGTGGACCCAAGTCCTCCAAGAGAACTTGCGCAATATGCTTAAATACCATGAAACCTGGACACGGGCATGCCATTTTTACTGCAGAGTGCTCTCACTCTTTTCACTTCCACTGCATTACTTCTAATGTAAAACACGGGAACCAAATTTGCCCAGTGTGCAGGGCAAAGTGGAAAGAAGTACCCTTTCAGAACCCTGCTTCTGATCTTTCACAGGGTATTCCAAGAATTAACCCAGTGAATTGGCCCCGAGATGATGCATGGATGACTGTTTTACGCCAAATTCCTCCACCACGCATTGACGTGAGCCAGCCTATTACGTCACTCTTGCACACTCCTGAACCAGTTATTTTTGACGATGATGAAAGCTTAGATAACCAACCTGACATTTCTAAGAATACGACATCTGTTGAAGATCAGAGTTCTAACAACAATTGTATTGGAATCGTAGAGGTCAAAACATATCCCGAAGTTCCAGCTGTTGAAAGATCAAGCTGTCATGATAACTTCAGTGTTTTAATTCATCTCAAGGCTCCTCTTACAAGTGCAAGACAGAATGGCAGCAGAAACCCAGTGTCTCAAAATTCTCGGGCCCCAGTAGACCTTGTTACAGTGCTTGACGTTAGTGGCAGCATGGCAGGTACAAAGCTTGCTTTGCTAAAACGAGCCATGGGGTTTGTGGTGCAGAACCTTGGTCCCTCTGATCGGCTTTCTGTTATTGCCTTCTCCTCTACAGCCCGCCGGCTCTTTCCCCTCCATCGCATGACTGACACTGGAAGGCAGCAAGCATTGCAGGCGGTAAACTCTCTTGTTTCAAATGGTGGGACAAACATTGCCGAGGCTCTTAGAAAAGGTACCAAGGTTTTAGTAGACCGCAAGTCAAAAAATCCTGTTTGCAGTATCATACTATTATCTGATGGGCAGGATACATATACTGTTACTAGTCCTGGTGGGATCCATTCTCGTACAGATTACCAATCACTTCTCCCAATCTCTATCCGTCGCAATAATGCAGCAGGCTTGCATATTCCAGTTCACACATTTGGATTTGGTGCAGACCATGATGCTGCCTCGATGCATTCAATCTCTGAGATTTCTGGGGGAACATTTTCTTTCATAGAAGCTGAAAGTGTGATTCAGGATGCATTCGCACAGTGCATTGGGGGCCTTCTGAGTGTAGTGGTGCAAGAGCTGCATGTCAGAATTGAGTGTGTTCACCCAAGTCTGCAATTGGGTTCAATAAAAGCTGGGAGTTATAGAACCAGCATGATGGCTGATTCAAGAATGGGATCTATTGATGCTGGAGACCTAGACCCAATTACGAAAGAAATGGTCAATTTGAATGAAGCTGGTGAAGTCATAATCGAAAGGCCCGAAGTAGCCGGACAACCAGTAGTGTCAATGGAAGTAGACAGGCAACGCAACAGGCTTCGTGCAGCAGAAGCAATGGCCGAGGCCAGAGTAGCAGCTGAGAATGCTGACTTGGCTGGTGCAGTTTCCCTCCTGGAGAGCTGTCGTCAGGCATTGGCTGAAACTACTTCTGCACGAGCCGGTGACCGCTTGTATGTTTCACTTGCAGCTGAGTTAAAGGAGATGCAAGAAAGAATGGGAAACCGGCGTGCATACGAAGAAACAGGTAGGGCTTACGTTTTATCAGGATTGAGTTCACACTCATGGCAGAGGGCAACTGCTCGAGGTGATTCCACAGATAGCACAAGCCTTCTGCAATCTTACCAAACCCCATCAATGGTAGACATGGTGACACGGTCTCAGACTATGCTACTAGGGAACCCATCACCTCGACGAACCCTCAGCAGTGTTAAGTCATTTCCAGCCAAATCACAGCCACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

691

Amino Acids

74.64

Weight (kDa)

8.49

Isoelectric Point (pI)

48.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 84 - 129 3.3e-07 Ring finger domain
zf-RING_11 PF17123 85 - 114 1.6e-07 RING-like zinc finger
zf-C3HC4 PF00097 85 - 128 1.1e-06 Zinc finger, C3HC4 type (RING finger)
VWA_CoxE PF05762 269 - 386 7.8e-09 VWA domain containing CoxE-like protein
VWA PF00092 275 - 456 1.8e-22 von Willebrand factor type A domain
VWA_3 PF13768 275 - 446 3.7e-19 von Willebrand factor type A domain
VWA_2 PF13519 276 - 382 9.5e-20 von Willebrand factor type A domain
WAV3_C PF25243 544 - 613 6.1e-07 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint PF14624 593 - 666 3.4e-19 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014999)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60710
fragaria_vesca FvH4_3g31730 FvH4_3g31730
malus_domestica MD03G1121000.v1.1 MD11G1139800.v1.1
pyrus_communis pycom11g11540
rosa_chinensis RchiOBHm_Chr5g0058461
rosa_laevigata RLG00000035222
rosa_multiflora Rmu_sc0000367.1_g000122
rosa_roxburghii Rroxscaffold_1G00022080
rosa_rugosa Rorug05G0314900
rosa_samantha Rh5AG381400 Rh5BG392900 Rh5CG417100 Rh5DG408100
rosa_wichuraiana Rw5G035930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 256
Acc36I ACCTGC 1 cut(s) 851
Acc65I GGTACC 1 cut(s) 1076
AccB1I GGYRCC 2 cut(s) 167, 1076
AccI GTMKAC 4 cut(s) 822, 1092, 1632, 1974
AciI CCGC 4 cut(s) 961, 1019, 1096, 1780
AclWI GGATC 3 cut(s) 1171, 1184, 1519
AcoI YGGCCR 1 cut(s) 1671
AcsI RAATTY 3 cut(s) 369, 504, 805
AcuI CTGAAG 1 cut(s) 715
AcyI GRCGYC 3 cut(s) 102, 126, 168
AfaI GTAC 4 cut(s) 405, 865, 1078, 1189
AfiI CCNNNNNNNGG 5 cut(s) 207, 227, 287, 927, 1969
AgsI TTSAA 8 cut(s) 43, 635, 707, 1038, 1305, 1461, 1503, 1564
AhdI GACNNNNNGTC 1 cut(s) 1099
AhlI ACTAGT 1 cut(s) 1163
AjiI CACGTC 1 cut(s) 526
AjnI CCWGG 3 cut(s) 280, 1168, 1725
AjuI GAANNNNNNNTTGG 2 cut(s) 1688, 1720
Alw21I GWGCWC 1 cut(s) 317
Alw26I GTCTC 4 cut(s) 96, 804, 1521, 1995
AlwI GGATC 3 cut(s) 1171, 1184, 1519
Ama87I CYCGRG 4 cut(s) 205, 471, 810, 1914
AoxI GGCC 6 cut(s) 467, 813, 1387, 1592, 1671, 1677
ApaI GGGCCC 1 cut(s) 817
ApeKI GCWGC 9 cut(s) 783, 852, 1003, 1235, 1289, 1414, 1658, 1688, 1799
ApoI RAATTY 3 cut(s) 369, 504, 805
Asp700I GAANNNNTTC 1 cut(s) 1330
Asp718I GGTACC 1 cut(s) 1076
AspLEI GCGC 2 cut(s) 170, 257
AspS9I GGNCC 7 cut(s) 230, 468, 813, 814, 917, 1387, 1593
AsuHPI GGTGA 6 cut(s) 1430, 1586, 1787, 1931, 1993, 2010
AvaI CYCGRG 4 cut(s) 205, 471, 810, 1914
AvaII GGWCC 2 cut(s) 230, 917
BaeGI GKGCMC 1 cut(s) 817
BaeI ACNNNNGTAYC 2 cut(s) 1068, 1101
BamHI GGATCC 1 cut(s) 1176
BanI GGYRCC 2 cut(s) 167, 1076
BanII GRGCYC 2 cut(s) 213, 817
BauI CACGAG 1 cut(s) 1767
Bbv12I GWGCWC 1 cut(s) 317
BbvCI CCTCAGC 1 cut(s) 2033
BbvI GCAGC 9 cut(s) 795, 864, 1015, 1247, 1276, 1401, 1670, 1700, 1811
BccI CCATC 5 cut(s) 987, 1133, 1486, 1972, 2023
BceAI ACGGC 2 cut(s) 113, 115
BciT130I CCWGG 3 cut(s) 282, 1170, 1727
BciVI GTATCC 1 cut(s) 1141
BcoDI GTCTC 4 cut(s) 96, 804, 1521, 1995
BcuI ACTAGT 1 cut(s) 1163
BfaI CTAG 4 cut(s) 114, 1164, 1532, 2006
BfmI CTRYAG 3 cut(s) 69, 306, 954
BfoI RGCGCY 1 cut(s) 171
BfuAI ACCTGC 1 cut(s) 851
BfuI GTATCC 1 cut(s) 1141
BisI GCNGC 9 cut(s) 784, 853, 1004, 1236, 1290, 1415, 1659, 1689, 1800
BlsI GCNGC 9 cut(s) 785, 854, 1005, 1237, 1291, 1416, 1660, 1690, 1801
Bme1390I CCNGG 3 cut(s) 282, 1170, 1727
Bme18I GGWCC 2 cut(s) 230, 917
BmeRI GACNNNNNGTC 1 cut(s) 1099
BmeT110I CYCGRG 4 cut(s) 205, 471, 810, 1914
BmgBI CACGTC 1 cut(s) 526
BmgT120I GGNCC 7 cut(s) 230, 468, 813, 814, 917, 1387, 1593
BmrFI CCNGG 3 cut(s) 282, 1170, 1727
BmrI ACTGGG 4 cut(s) 371, 452, 788, 812
BmsI GCATC 6 cut(s) 469, 1276, 1287, 1354, 1510, 1806
BmuI ACTGGG 4 cut(s) 371, 452, 788, 812
BoxI GACNNNNGTC 1 cut(s) 235
BpmI CTGGAG 2 cut(s) 1545, 1748
Bpu10I CCTNAGC 1 cut(s) 2033
BpuEI CTTGAG 1 cut(s) 734
BsaHI GRCGYC 3 cut(s) 102, 126, 168
BsaI GGTCTC 2 cut(s) 1521, 1995
BsaJI CCNNGG 6 cut(s) 204, 891, 913, 1059, 1080, 1674
BsaWI WCCGGW 1 cut(s) 213
BsaXI ACNNNNNCTCC 2 cut(s) 178, 208
Bsc4I CCNNNNNNNGG 5 cut(s) 207, 227, 287, 927, 1969
Bse118I RCCGGY 4 cut(s) 174, 963, 1772, 1836
Bse1I ACTGG 9 cut(s) 163, 377, 458, 566, 794, 818, 1000, 1253, 1613
Bse3DI GCAATG 3 cut(s) 1010, 1053, 1674
BseBI CCWGG 3 cut(s) 282, 1170, 1727
BseDI CCNNGG 6 cut(s) 204, 891, 913, 1059, 1080, 1674
BseGI GGATG 2 cut(s) 492, 1369
BseLI CCNNNNNNNGG 5 cut(s) 207, 227, 287, 927, 1969
BseMI GCAATG 3 cut(s) 1010, 1053, 1674
BseMII CTCAG 6 cut(s) 1302, 1385, 1683, 1794, 2006, 2047
BseNI ACTGG 9 cut(s) 163, 377, 458, 566, 794, 818, 1000, 1253, 1613
BseRI GAGGAG 4 cut(s) 130, 193, 747, 940
BseSI GKGCMC 1 cut(s) 817
BseXI GCAGC 9 cut(s) 795, 864, 1015, 1247, 1276, 1401, 1670, 1700, 1811
BseYI CCCAGC 1 cut(s) 1469
BsgI GTGCAG 6 cut(s) 403, 926, 1296, 1677, 1734, 1749
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 6 cut(s) 469, 815, 1389, 1594, 1673, 1679
BshNI GGYRCC 2 cut(s) 167, 1076
BsiEI CGRYCG 1 cut(s) 175
BsiHKAI GWGCWC 1 cut(s) 317
BsiHKCI CYCGRG 4 cut(s) 205, 471, 810, 1914
BsiSI CCGG 6 cut(s) 175, 214, 964, 1605, 1773, 1837
BslFI GGGAC 2 cut(s) 903, 1060
BslI CCNNNNNNNGG 5 cut(s) 207, 227, 287, 927, 1969
BsmAI GTCTC 4 cut(s) 96, 804, 1521, 1995
BsmBI CGTCTC 1 cut(s) 96
BsmFI GGGAC 2 cut(s) 903, 1060
BsmI GAATGC 3 cut(s) 1300, 1367, 1702
BsnI GGCC 6 cut(s) 469, 815, 1389, 1594, 1673, 1679
Bso31I GGTCTC 2 cut(s) 1521, 1995
BsoBI CYCGRG 4 cut(s) 205, 471, 810, 1914
Bsp120I GGGCCC 1 cut(s) 813
Bsp1286I GDGCHC 3 cut(s) 213, 317, 817
Bsp143I GATC 6 cut(s) 427, 637, 710, 925, 1176, 1511
Bsp19I CCATGG 1 cut(s) 891
BspACI CCGC 4 cut(s) 961, 1019, 1096, 1780
BspANI GGCC 6 cut(s) 469, 815, 1389, 1594, 1673, 1679
BspCNI CTCAG 6 cut(s) 1303, 1386, 1684, 1795, 2005, 2046
BspHI TCATGA 1 cut(s) 721
BspMAI CTGCAG 2 cut(s) 73, 310
BspMI ACCTGC 1 cut(s) 851
BspPI GGATC 3 cut(s) 1171, 1184, 1519
BspT107I GGYRCC 2 cut(s) 167, 1076
BspTNI GGTCTC 2 cut(s) 1521, 1995
BsrDI GCAATG 3 cut(s) 1010, 1053, 1674
BsrFI RCCGGY 4 cut(s) 174, 963, 1772, 1836
BsrI ACTGG 9 cut(s) 163, 377, 458, 566, 794, 818, 1000, 1253, 1613
BssAI RCCGGY 4 cut(s) 174, 963, 1772, 1836
BssECI CCNNGG 6 cut(s) 204, 891, 913, 1059, 1080, 1674
BssMI GATC 6 cut(s) 427, 637, 710, 925, 1176, 1511
BssNI GRCGYC 3 cut(s) 102, 126, 168
BssSI CACGAG 1 cut(s) 1767
BssT1I CCWWGG 3 cut(s) 891, 913, 1080
Bst2BI CACGAG 1 cut(s) 1767
Bst2UI CCWGG 3 cut(s) 282, 1170, 1727
Bst4CI ACNGT 6 cut(s) 90, 493, 835, 1159, 1377, 1989
BstACI GRCGYC 3 cut(s) 102, 126, 168
BstAPI GCANNNNNTGC 2 cut(s) 1012, 1414
BstDEI CTNAG 9 cut(s) 592, 615, 1067, 1311, 1394, 1692, 1803, 1992, 2033
BstDSI CCRYGG 1 cut(s) 891
BstEII GGTNACC 1 cut(s) 1775
BstF5I GGATG 2 cut(s) 492, 1369
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 2 cut(s) 170, 257
BstKTI GATC 6 cut(s) 430, 640, 713, 928, 1179, 1514
BstMAI GTCTC 4 cut(s) 96, 804, 1521, 1995
BstMBI GATC 6 cut(s) 427, 637, 710, 925, 1176, 1511
BstMCI CGRYCG 1 cut(s) 175
BstNI CCWGG 3 cut(s) 282, 1170, 1727
BstNSI RCATGY 2 cut(s) 296, 1421
BstPAI GACNNNNGTC 1 cut(s) 235
BstPI GGTNACC 1 cut(s) 1775
BstSCI CCNGG 3 cut(s) 280, 1168, 1725
BstSFI CTRYAG 3 cut(s) 69, 306, 954
BstSLI GKGCMC 1 cut(s) 817
BstV1I GCAGC 9 cut(s) 795, 864, 1015, 1247, 1276, 1401, 1670, 1700, 1811
BstX2I RGATCY 2 cut(s) 1176, 1511
BstXI CCANNNNNNTGG 2 cut(s) 465, 1492
BstYI RGATCY 2 cut(s) 1176, 1511
BsuI GTATCC 1 cut(s) 1141
BsuRI GGCC 6 cut(s) 469, 815, 1389, 1594, 1673, 1679
BtgI CCRYGG 1 cut(s) 891
BtgZI GCGATG 1 cut(s) 965
BtrI CACGTC 1 cut(s) 526
BtsCI GGATG 2 cut(s) 492, 1369
BtsI GCAGTG 2 cut(s) 334, 2044
BtsIMutI CAGTG 9 cut(s) 334, 384, 465, 739, 801, 840, 993, 1382, 2044
BveI ACCTGC 1 cut(s) 851
CciI TCATGA 1 cut(s) 721
CfoI GCGC 2 cut(s) 170, 257
Cfr10I RCCGGY 4 cut(s) 174, 963, 1772, 1836
Cfr13I GGNCC 7 cut(s) 230, 468, 813, 814, 917, 1387, 1593
CseI GACGC 2 cut(s) 91, 134
Csp6I GTAC 4 cut(s) 404, 864, 1077, 1188
CviQI GTAC 4 cut(s) 404, 864, 1077, 1188
DdeI CTNAG 9 cut(s) 592, 615, 1067, 1311, 1394, 1692, 1803, 1992, 2033
DinI GGCGCC 1 cut(s) 169
DpnI GATC 6 cut(s) 429, 639, 712, 927, 1178, 1513
DpnII GATC 6 cut(s) 427, 637, 710, 925, 1176, 1511
DriI GACNNNNNGTC 1 cut(s) 1099
EaeI YGGCCR 1 cut(s) 1671
Eam1105I GACNNNNNGTC 1 cut(s) 1099
Eco130I CCWWGG 3 cut(s) 891, 913, 1080
Eco24I GRGCYC 2 cut(s) 213, 817
Eco31I GGTCTC 2 cut(s) 1521, 1995
Eco47I GGWCC 2 cut(s) 230, 917
Eco57I CTGAAG 1 cut(s) 715
Eco88I CYCGRG 4 cut(s) 205, 471, 810, 1914
Eco91I GGTNACC 1 cut(s) 1775
EcoO109I RGGNCCY 2 cut(s) 814, 1387
EcoO65I GGTNACC 1 cut(s) 1775
EcoRII CCWGG 3 cut(s) 280, 1168, 1725
EcoT14I CCWWGG 3 cut(s) 891, 913, 1080
EcoT22I ATGCAT 3 cut(s) 484, 1302, 1369
EcoT38I GRGCYC 2 cut(s) 213, 817
EgeI GGCGCC 1 cut(s) 169
EheI GGCGCC 1 cut(s) 169
ErhI CCWWGG 3 cut(s) 891, 913, 1080
Esp3I CGTCTC 1 cut(s) 96
FalI AAGNNNNNCTT 2 cut(s) 12, 44
FaqI GGGAC 2 cut(s) 903, 1060
FauI CCCGC 1 cut(s) 968
FblI GTMKAC 4 cut(s) 822, 1092, 1632, 1974
Fnu4HI GCNGC 9 cut(s) 784, 853, 1004, 1236, 1290, 1415, 1659, 1689, 1800
FokI GGATG 2 cut(s) 499, 1376
FriOI GRGCYC 2 cut(s) 213, 817
Fsp4HI GCNGC 9 cut(s) 784, 853, 1004, 1236, 1290, 1415, 1659, 1689, 1800
FspBI CTAG 4 cut(s) 114, 1164, 1532, 2006
FspI TGCGCA 1 cut(s) 256
GlaI GCGC 2 cut(s) 169, 256
GluI GCNGC 9 cut(s) 784, 853, 1004, 1236, 1290, 1415, 1659, 1689, 1800
GsaI CCCAGC 1 cut(s) 1473
GsuI CTGGAG 2 cut(s) 1545, 1748
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 6 cut(s) 469, 815, 1389, 1594, 1673, 1679
HapII CCGG 6 cut(s) 175, 214, 964, 1605, 1773, 1837
HgaI GACGC 2 cut(s) 91, 134
HhaI GCGC 2 cut(s) 170, 257
Hin1I GRCGYC 3 cut(s) 102, 126, 168
Hin6I GCGC 2 cut(s) 168, 255
HinP1I GCGC 2 cut(s) 168, 255
HindIII AAGCTT 3 cut(s) 26, 589, 869
HinfI GANTC 6 cut(s) 77, 110, 666, 1357, 1499, 1922
HpaII CCGG 6 cut(s) 175, 214, 964, 1605, 1773, 1837
HphI GGTGA 6 cut(s) 1430, 1586, 1787, 1931, 1993, 2010
Hpy166II GTNNAC 9 cut(s) 230, 823, 1024, 1093, 1258, 1438, 1633, 1889, 1975
Hpy188III TCNNGA 7 cut(s) 114, 560, 689, 722, 1361, 1503, 1878
Hpy8I GTNNAC 9 cut(s) 230, 823, 1024, 1093, 1258, 1438, 1633, 1889, 1975
Hpy99I CGWCG 5 cut(s) 107, 110, 128, 1227, 2028
HpyAV CCTTC 4 cut(s) 955, 993, 1400, 1952
HpyCH4III ACNGT 6 cut(s) 90, 493, 835, 1159, 1377, 1989
HpyCH4IV ACGT 5 cut(s) 525, 542, 843, 1869, 2071
HpyF3I CTNAG 9 cut(s) 592, 615, 1067, 1311, 1394, 1692, 1803, 1992, 2033
HpySE526I ACGT 5 cut(s) 525, 542, 843, 1869, 2071
Hsp92I GRCGYC 3 cut(s) 102, 126, 168
HspAI GCGC 2 cut(s) 168, 255
KasI GGCGCC 1 cut(s) 167
KpnI GGTACC 1 cut(s) 1080
KroI GCCGGC 1 cut(s) 963
KroNI GCCGGC 1 cut(s) 965
Kzo9I GATC 6 cut(s) 427, 637, 710, 925, 1176, 1511
LmnI GCTCC 3 cut(s) 6, 216, 760
Lsp1109I GCAGC 9 cut(s) 795, 864, 1015, 1247, 1276, 1401, 1670, 1700, 1811
LweI GCATC 6 cut(s) 469, 1276, 1287, 1354, 1510, 1806
MaeI CTAG 4 cut(s) 114, 1164, 1532, 2006
MaeII ACGT 5 cut(s) 525, 542, 843, 1869, 2071
MaeIII GTNAC 5 cut(s) 543, 829, 1159, 1775, 1981
MalI GATC 6 cut(s) 429, 639, 712, 927, 1178, 1513
MboI GATC 6 cut(s) 427, 637, 710, 925, 1176, 1511
MboII GAAGA 3 cut(s) 126, 647, 1862
MfeI CAATTG 2 cut(s) 655, 1451
MflI RGATCY 2 cut(s) 1176, 1511
MhlI GDGCHC 3 cut(s) 213, 317, 817
Mly113I GGCGCC 1 cut(s) 168
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 2 cut(s) 147, 179
Mph1103I ATGCAT 3 cut(s) 484, 1302, 1369
MroNI GCCGGC 1 cut(s) 963
MroXI GAANNNNTTC 1 cut(s) 1330
MseI TTAA 6 cut(s) 266, 453, 740, 1808, 2043, 2074
MslI CAYNNNNRTG 3 cut(s) 291, 524, 1894
MspA1I CMGCKG 3 cut(s) 701, 1691, 1802
MspI CCGG 6 cut(s) 175, 214, 964, 1605, 1773, 1837
MspR9I CCNGG 3 cut(s) 282, 1170, 1727
MunI CAATTG 2 cut(s) 655, 1451
Mva1269I GAATGC 3 cut(s) 1300, 1367, 1702
MvaI CCWGG 3 cut(s) 282, 1170, 1727
NaeI GCCGGC 1 cut(s) 965
NarI GGCGCC 1 cut(s) 168
NcoI CCATGG 1 cut(s) 891
NdeII GATC 6 cut(s) 427, 637, 710, 925, 1176, 1511
NgoMIV GCCGGC 1 cut(s) 963
NmeAIII GCCGAG 2 cut(s) 1084, 1699
NmuCI GTSAC 3 cut(s) 543, 1775, 1981
NsbI TGCGCA 1 cut(s) 256
NsiI ATGCAT 3 cut(s) 484, 1302, 1369
NspI RCATGY 2 cut(s) 296, 1421
PaeI GCATGC 1 cut(s) 296
PaeR7I CTCGAG 1 cut(s) 1914
PagI TCATGA 1 cut(s) 721
PcsI WCGNNNNNNNCGW 3 cut(s) 105, 194, 1593
PctI GAATGC 3 cut(s) 1300, 1367, 1702
PdiI GCCGGC 1 cut(s) 965
PdmI GAANNNNTTC 1 cut(s) 1330
PfeI GAWTC 5 cut(s) 77, 666, 1357, 1499, 1922
PflFI GACNNNGTC 1 cut(s) 1987
PfoI TCCNGGA 1 cut(s) 1725
PkrI GCNGC 9 cut(s) 785, 854, 1005, 1237, 1291, 1416, 1660, 1690, 1801
PleI GAGTC 1 cut(s) 118
PluTI GGCGCC 1 cut(s) 171
PpsI GAGTC 1 cut(s) 118
PshAI GACNNNNGTC 1 cut(s) 235
Psp6I CCWGG 3 cut(s) 280, 1168, 1725
PspEI GGTNACC 1 cut(s) 1775
PspFI CCCAGC 1 cut(s) 1469
PspGI CCWGG 3 cut(s) 280, 1168, 1725
PspOMI GGGCCC 1 cut(s) 813
PspPI GGNCC 7 cut(s) 230, 468, 813, 814, 917, 1387, 1593
PspXI VCTCGAGB 1 cut(s) 1914
PstI CTGCAG 2 cut(s) 73, 310
PsuI RGATCY 2 cut(s) 1176, 1511
PsyI GACNNNGTC 1 cut(s) 1987
PvuII CAGCTG 3 cut(s) 701, 1691, 1802
RsaI GTAC 4 cut(s) 405, 865, 1078, 1189
RsaNI GTAC 4 cut(s) 404, 864, 1077, 1188
RseI CAYNNNNRTG 3 cut(s) 291, 524, 1894
SaqAI TTAA 6 cut(s) 266, 453, 740, 1808, 2043, 2074
SatI GCNGC 9 cut(s) 784, 853, 1004, 1236, 1290, 1415, 1659, 1689, 1800
Sau3AI GATC 6 cut(s) 427, 637, 710, 925, 1176, 1511
Sau96I GGNCC 7 cut(s) 230, 468, 813, 814, 917, 1387, 1593
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 3 cut(s) 282, 1170, 1727
SduI GDGCHC 3 cut(s) 213, 317, 817
SfaNI GCATC 6 cut(s) 469, 1276, 1287, 1354, 1510, 1806
SfcI CTRYAG 3 cut(s) 69, 306, 954
SfoI GGCGCC 1 cut(s) 169
Sfr274I CTCGAG 1 cut(s) 1914
SinI GGWCC 2 cut(s) 230, 917
SlaI CTCGAG 1 cut(s) 1914
SmiMI CAYNNNNRTG 3 cut(s) 291, 524, 1894
SmlI CTYRAG 2 cut(s) 749, 1914
SmoI CTYRAG 2 cut(s) 749, 1914
SpeI ACTAGT 1 cut(s) 1163
SphI GCATGC 1 cut(s) 296
SsiI CCGC 4 cut(s) 961, 1019, 1096, 1780
SspDI GGCGCC 1 cut(s) 167
SspMI CTAG 4 cut(s) 114, 1164, 1532, 2006
StyD4I CCNGG 3 cut(s) 280, 1168, 1725
StyI CCWWGG 3 cut(s) 891, 913, 1080
TaaI ACNGT 6 cut(s) 90, 493, 835, 1159, 1377, 1989
TaiI ACGT 5 cut(s) 528, 545, 846, 1872, 2074
TaqI TCGA 5 cut(s) 108, 1295, 1587, 1915, 2023
TfiI GAWTC 5 cut(s) 77, 666, 1357, 1499, 1922
Tru1I TTAA 6 cut(s) 266, 453, 740, 1808, 2043, 2074
Tru9I TTAA 6 cut(s) 266, 453, 740, 1808, 2043, 2074
TscAI CASTG 9 cut(s) 341, 384, 465, 739, 801, 840, 1000, 1382, 2044
TseFI GTSAC 3 cut(s) 543, 1775, 1981
TseI GCWGC 9 cut(s) 783, 852, 1003, 1235, 1289, 1414, 1658, 1688, 1799
Tsp45I GTSAC 3 cut(s) 543, 1775, 1981
TspDTI ATGAA 5 cut(s) 290, 600, 734, 1327, 1581
TspGWI ACGGA 2 cut(s) 186, 1211
TspRI CASTG 9 cut(s) 341, 384, 465, 739, 801, 840, 1000, 1382, 2044
Tth111I GACNNNGTC 1 cut(s) 1987
VpaK11BI GGWCC 2 cut(s) 230, 917
XapI RAATTY 3 cut(s) 369, 504, 805
XbaI TCTAGA 1 cut(s) 113
XceI RCATGY 2 cut(s) 296, 1421
XcmI CCANNNNNNNNNTGG 1 cut(s) 898
XhoI CTCGAG 1 cut(s) 1914
XmiI GTMKAC 4 cut(s) 822, 1092, 1632, 1974
XmnI GAANNNNTTC 1 cut(s) 1330
XspI CTAG 4 cut(s) 114, 1164, 1532, 2006
Zsp2I ATGCAT 3 cut(s) 484, 1302, 1369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.