Rh5DG408100

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0014999
Physical Location & Seq
Forward (+)
64630677 .. 64635093
4417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG408100.1

Sequence Viewer

Length: 2175 bp
ATGGGGAGCAAATGGAGGAAAGCCAAGCTTGCTCTTGGCTTGAACACTTGTCTTTATGTTCCTCAAACTGCAGAGGAATCATCATCACCGTCTCCAAACGGCATCGTTGAGTCTAGACACTCCGACGCCGTTTCTTCCTCCTCTCTGCTTTCTCCGACTGGTATTGGCTCCGACCGGCGACCCACCACGCCCACTCCGTCCTCCTCGGGGCTCCGGTTGCCCAAAAGTGGACCCAAGTCCTCCAAGAGAACTTGCGCAATATGCTTAAATACCATGAAACCTGGACACGGGCATGCCATTTTTACTGCAGAGTGCTCTCACTCTTTTCACTTCCACTGCATTACTTCTAATGTAAAACATGGGAACCAAATTTGCCCAGTTTGCAGGGCAAAGTGGAAAGAAGTCCCCTTTCAGAACCCTGCTTCTGATCTTTCACAGGGTATTCCAAGAATTAACCCAGTGAATTGGCCTCGAGATGATGCATGGATGACTGTTTTACGCCAAATTCCTCCACCACGCGTTGATGTGAGCCGGCCTATTACGTCACTCTTGCACACTCCTGAACCAGTTATTTTTGACGATGATGAAAGCTTAGATAACCAACCAGACATTTCTAAGAATACGACATCTGTTGAAGAACAGAGTTCTAACAACAATTGTATTGGAATCGTAGAGGTCAAAACATATCCCGAAGTTCCAGCTGTTGAAAGATCAAGCTCTCATGATAACTTCAGTGTTTTAATTCATCTCAAGGCTCCTCTTACAAGTGCAAGACAAAATGGCAGCAGAAACCCAGTGTCTCAAAATTCTCGGGCCCCAGTAGACCTTATTACAGTGCTTGATGTTAGTGGCAGCATGGCAGGTACAAAGCTTGCTTTGCTAAAACGAGCCATGGGGTTTGTGGTGCAGAACCTTGGTCCTTCTGATCGGCTTTCTGTTATTGCCTTCTCCTCTACAGCCCGCCGGCTCTTTCCCCTTCGTCGCATGACTGACACCGGAAGGCAGCAAGCATTGCAGGCTGTAAACTCTCTTGTTTCAAATGGTGGGACAAACATTGCCGAGGCTCTTAGAAAAGGTACCAAGGTTTTAGTAGACCGCAAGTCAAAAAATCCTGTTTGCAGTATCATACTATTATCTGATGGGCAGGATACATATACTGTTACTAGTCCATGTGGGATCCATCCTCGTACAGATTACCAATCACTTCTCCCAATCTCTATCCGTCGCAATAATGCAGCAGGCTTGCATATTCCAGTTCACACATTTGGATTTGGTGCAGACCATGATGCTGCCTCGATGCATTCAATCTCTGAGATTTCTGGGGGAACATTTTCTTTCATAGAAGCTGAAAGTGTGATTCAGGATGCATTCGCACAGTGCATTGGGGGCCTTCTGAGTGTAGTGGTGCAAGAACTGCATGTCAGAATTGAGTGTGTTCACCCAAGTCTGCAATTGGGTTCAATAAAAGCTGGGAGTTATAGAACCAGCATGACGGCTGATGCAAGAATGGGATCTATTGATGCTGGAGACCTGTATGCTGAAGAAGAGAGGGATTTTTTGGTGACAATGAATATTCCTGTCGACCAGTCTAGTAATGAGATGTCACTGGTAAAGGTTAGATGTCTTTACAGAGACCCAATTACGAAAGAAATGGTCAATTTGAATGAAGCTGGTGAAGTCACAATCGAAAGGCCCGAAGTAGCCGGACAACTAGTAGTGTCAATGGAAGTAGACAGGCAACGCAACAGGCTTCGTGCGGCAGAAGCAATGGCCGAGGCCAGAGTAGCAGCTGAGAATGCTGATTTGGCTGGTGCAGTTTCCCTCCTGGAGAGCTGTCGTCAGGCATTGGCTGAAACTACTTCTGCACGAGCCGGTGACCGCTTGTGTGTTGCACTTGCAGCTGAGTTAAAGGAGATGCAAGAAAGAATGGGAAACCGTCGTGCATATGAAGAAACAGGTAGGGCTTACGTTTTATCAGGATTGAGTTCACACTCATGGCAGAGGGCAACTGCTCGAGGTGATTCCACAGATAGCACAAGCCTTCTGCAATCTTACCAAACCCCATCAATGGTAGACATGGTGACACGGTCTCAGACTATGCTACTAGGGAACCCATCACCTCGACGAACCCTCAGCAGTGTTAAGTCATTTCCAGCCAAATCACAGCCACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

724

Amino Acids

78.56

Weight (kDa)

8.43

Isoelectric Point (pI)

48.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 84 - 129 3.5e-07 Ring finger domain
zf-RING_11 PF17123 85 - 114 1.7e-07 RING-like zinc finger
zf-C3HC4 PF00097 85 - 128 1.2e-06 Zinc finger, C3HC4 type (RING finger)
VWA_CoxE PF05762 269 - 386 2e-08 VWA domain containing CoxE-like protein
VWA PF00092 275 - 456 3.3e-22 von Willebrand factor type A domain
VWA_3 PF13768 275 - 446 9.5e-19 von Willebrand factor type A domain
VWA_2 PF13519 276 - 382 1.3e-19 von Willebrand factor type A domain
WAV3_C PF25243 577 - 646 7.2e-07 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint PF14624 626 - 699 5.6e-20 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014999)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60710
fragaria_vesca FvH4_3g31730 FvH4_3g31730
malus_domestica MD03G1121000.v1.1 MD11G1139800.v1.1
pyrus_communis pycom11g11540
rosa_chinensis RchiOBHm_Chr5g0058461
rosa_laevigata RLG00000035222
rosa_multiflora Rmu_sc0000367.1_g000122
rosa_roxburghii Rroxscaffold_1G00022080
rosa_rugosa Rorug05G0314900
rosa_samantha Rh5AG381400 Rh5BG392900 Rh5CG417100 Rh5DG408100
rosa_wichuraiana Rw5G035930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 256
Acc36I ACCTGC 1 cut(s) 851
Acc65I GGTACC 1 cut(s) 1076
AccB1I GGYRCC 1 cut(s) 1076
AccI GTMKAC 5 cut(s) 822, 1092, 1581, 1731, 2073
AccII CGCG 1 cut(s) 519
AciI CCGC 4 cut(s) 961, 1096, 1757, 1879
AclWI GGATC 3 cut(s) 1171, 1184, 1519
AcoI YGGCCR 1 cut(s) 1770
AcsI RAATTY 3 cut(s) 369, 504, 805
AcuI CTGAAG 2 cut(s) 715, 1560
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 3 cut(s) 865, 1078, 1189
AfiI CCNNNNNNNGG 4 cut(s) 207, 227, 287, 2068
AflIII ACRYGT 1 cut(s) 517
AgsI TTSAA 7 cut(s) 43, 635, 707, 1038, 1305, 1461, 1663
AhdI GACNNNNNGTC 1 cut(s) 1099
AhlI ACTAGT 2 cut(s) 1163, 1711
AjnI CCWGG 2 cut(s) 280, 1824
AjuI GAANNNNNNNTTGG 2 cut(s) 1787, 1819
Alw21I GWGCWC 1 cut(s) 317
Alw26I GTCTC 5 cut(s) 96, 804, 1521, 1626, 2094
AlwI GGATC 3 cut(s) 1171, 1184, 1519
Ama87I CYCGRG 4 cut(s) 205, 471, 810, 2013
AoxI GGCC 7 cut(s) 467, 533, 813, 1387, 1691, 1770, 1776
ApaI GGGCCC 1 cut(s) 817
ApeKI GCWGC 7 cut(s) 783, 852, 1003, 1235, 1289, 1787, 1898
ApoI RAATTY 3 cut(s) 369, 504, 805
Asp700I GAANNNNTTC 1 cut(s) 1330
Asp718I GGTACC 1 cut(s) 1076
AspLEI GCGC 1 cut(s) 257
AspS9I GGNCC 6 cut(s) 230, 813, 814, 917, 1387, 1692
AsuHPI GGTGA 8 cut(s) 78, 1430, 1573, 1685, 1886, 2030, 2092, 2109
AvaI CYCGRG 4 cut(s) 205, 471, 810, 2013
AvaII GGWCC 2 cut(s) 230, 917
BaeGI GKGCMC 1 cut(s) 817
BaeI ACNNNNGTAYC 2 cut(s) 1068, 1101
BamHI GGATCC 1 cut(s) 1176
BanI GGYRCC 1 cut(s) 1076
BanII GRGCYC 2 cut(s) 213, 817
BauI CACGAG 1 cut(s) 1866
Bbv12I GWGCWC 1 cut(s) 317
BbvCI CCTCAGC 1 cut(s) 2132
BbvI GCAGC 7 cut(s) 795, 864, 1015, 1247, 1276, 1799, 1910
BccI CCATC 4 cut(s) 1133, 1188, 2071, 2122
BceAI ACGGC 3 cut(s) 113, 115, 1509
BciT130I CCWGG 2 cut(s) 282, 1826
BciVI GTATCC 1 cut(s) 1141
BcoDI GTCTC 5 cut(s) 96, 804, 1521, 1626, 2094
BcuI ACTAGT 2 cut(s) 1163, 1711
BfaI CTAG 5 cut(s) 114, 1164, 1590, 1712, 2105
BfmI CTRYAG 3 cut(s) 69, 306, 954
BfuAI ACCTGC 1 cut(s) 851
BfuI GTATCC 1 cut(s) 1141
BisI GCNGC 8 cut(s) 784, 853, 1004, 1236, 1290, 1758, 1788, 1899
BlsI GCNGC 8 cut(s) 785, 854, 1005, 1237, 1291, 1759, 1789, 1900
Bme1390I CCNGG 2 cut(s) 282, 1826
Bme18I GGWCC 2 cut(s) 230, 917
BmeRI GACNNNNNGTC 1 cut(s) 1099
BmeT110I CYCGRG 4 cut(s) 205, 471, 810, 2013
BmgT120I GGNCC 6 cut(s) 230, 813, 814, 917, 1387, 1692
BmrFI CCNGG 2 cut(s) 282, 1826
BmrI ACTGGG 4 cut(s) 371, 452, 788, 812
BmsI GCATC 8 cut(s) 111, 469, 1276, 1287, 1354, 1489, 1510, 1905
BmuI ACTGGG 4 cut(s) 371, 452, 788, 812
BoxI GACNNNNGTC 1 cut(s) 235
BpmI CTGGAG 2 cut(s) 1545, 1847
Bpu10I CCTNAGC 1 cut(s) 2132
BpuEI CTTGAG 1 cut(s) 734
BsaHI GRCGYC 1 cut(s) 126
BsaI GGTCTC 3 cut(s) 1521, 1626, 2094
BsaJI CCNNGG 6 cut(s) 204, 891, 913, 1059, 1080, 1773
BsaWI WCCGGW 2 cut(s) 213, 995
BsaXI ACNNNNNCTCC 2 cut(s) 178, 208
Bsc4I CCNNNNNNNGG 4 cut(s) 207, 227, 287, 2068
Bse118I RCCGGY 4 cut(s) 174, 531, 963, 1871
Bse1I ACTGG 9 cut(s) 163, 377, 458, 566, 794, 818, 1253, 1585, 1611
Bse3DI GCAATG 3 cut(s) 1010, 1053, 1773
BseBI CCWGG 2 cut(s) 282, 1826
BseDI CCNNGG 6 cut(s) 204, 891, 913, 1059, 1080, 1773
BseGI GGATG 3 cut(s) 492, 1180, 1369
BseLI CCNNNNNNNGG 4 cut(s) 207, 227, 287, 2068
BseMI GCAATG 3 cut(s) 1010, 1053, 1773
BseMII CTCAG 6 cut(s) 1302, 1385, 1782, 1893, 2105, 2146
BseNI ACTGG 9 cut(s) 163, 377, 458, 566, 794, 818, 1253, 1585, 1611
BseRI GAGGAG 4 cut(s) 130, 193, 747, 940
BseSI GKGCMC 1 cut(s) 817
BseXI GCAGC 7 cut(s) 795, 864, 1015, 1247, 1276, 1799, 1910
BseYI CCCAGC 1 cut(s) 1469
BsgI GTGCAG 4 cut(s) 926, 1296, 1833, 1848
Bsh1236I CGCG 1 cut(s) 519
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 7 cut(s) 469, 535, 815, 1389, 1693, 1772, 1778
BshNI GGYRCC 1 cut(s) 1076
BsiEI CGRYCG 1 cut(s) 175
BsiHKAI GWGCWC 1 cut(s) 317
BsiHKCI CYCGRG 4 cut(s) 205, 471, 810, 2013
BsiSI CCGG 7 cut(s) 175, 214, 532, 964, 996, 1704, 1872
BslFI GGGAC 2 cut(s) 389, 1060
BslI CCNNNNNNNGG 4 cut(s) 207, 227, 287, 2068
BsmAI GTCTC 5 cut(s) 96, 804, 1521, 1626, 2094
BsmBI CGTCTC 1 cut(s) 96
BsmFI GGGAC 2 cut(s) 389, 1060
BsmI GAATGC 3 cut(s) 1300, 1367, 1801
BsnI GGCC 7 cut(s) 469, 535, 815, 1389, 1693, 1772, 1778
Bso31I GGTCTC 3 cut(s) 1521, 1626, 2094
BsoBI CYCGRG 4 cut(s) 205, 471, 810, 2013
Bsp120I GGGCCC 1 cut(s) 813
Bsp1286I GDGCHC 3 cut(s) 213, 317, 817
Bsp143I GATC 5 cut(s) 427, 710, 925, 1176, 1511
Bsp19I CCATGG 1 cut(s) 891
BspACI CCGC 4 cut(s) 961, 1096, 1757, 1879
BspANI GGCC 7 cut(s) 469, 535, 815, 1389, 1693, 1772, 1778
BspCNI CTCAG 6 cut(s) 1303, 1386, 1783, 1894, 2104, 2145
BspFNI CGCG 1 cut(s) 519
BspHI TCATGA 1 cut(s) 721
BspMAI CTGCAG 2 cut(s) 73, 310
BspMI ACCTGC 1 cut(s) 851
BspPI GGATC 3 cut(s) 1171, 1184, 1519
BspT107I GGYRCC 1 cut(s) 1076
BspTNI GGTCTC 3 cut(s) 1521, 1626, 2094
BsrDI GCAATG 3 cut(s) 1010, 1053, 1773
BsrFI RCCGGY 4 cut(s) 174, 531, 963, 1871
BsrI ACTGG 9 cut(s) 163, 377, 458, 566, 794, 818, 1253, 1585, 1611
BssAI RCCGGY 4 cut(s) 174, 531, 963, 1871
BssECI CCNNGG 6 cut(s) 204, 891, 913, 1059, 1080, 1773
BssMI GATC 5 cut(s) 427, 710, 925, 1176, 1511
BssNI GRCGYC 1 cut(s) 126
BssSI CACGAG 1 cut(s) 1866
BssT1I CCWWGG 3 cut(s) 891, 913, 1080
Bst2BI CACGAG 1 cut(s) 1866
Bst2UI CCWGG 2 cut(s) 282, 1826
Bst4CI ACNGT 7 cut(s) 90, 493, 835, 1159, 1377, 1937, 2088
Bst6I CTCTTC 1 cut(s) 1539
BstACI GRCGYC 1 cut(s) 126
BstAPI GCANNNNNTGC 2 cut(s) 1012, 1414
BstDEI CTNAG 9 cut(s) 592, 615, 1067, 1311, 1394, 1791, 1902, 2091, 2132
BstDSI CCRYGG 1 cut(s) 891
BstEII GGTNACC 1 cut(s) 1874
BstF5I GGATG 3 cut(s) 492, 1180, 1369
BstFNI CGCG 1 cut(s) 519
BstHHI GCGC 1 cut(s) 257
BstKTI GATC 5 cut(s) 430, 713, 928, 1179, 1514
BstMAI GTCTC 5 cut(s) 96, 804, 1521, 1626, 2094
BstMBI GATC 5 cut(s) 427, 710, 925, 1176, 1511
BstMCI CGRYCG 1 cut(s) 175
BstNI CCWGG 2 cut(s) 282, 1826
BstNSI RCATGY 2 cut(s) 296, 1421
BstPAI GACNNNNGTC 1 cut(s) 235
BstPI GGTNACC 1 cut(s) 1874
BstSCI CCNGG 2 cut(s) 280, 1824
BstSFI CTRYAG 3 cut(s) 69, 306, 954
BstSLI GKGCMC 1 cut(s) 817
BstUI CGCG 1 cut(s) 519
BstV1I GCAGC 7 cut(s) 795, 864, 1015, 1247, 1276, 1799, 1910
BstX2I RGATCY 2 cut(s) 1176, 1511
BstXI CCANNNNNNTGG 1 cut(s) 465
BstYI RGATCY 2 cut(s) 1176, 1511
BsuI GTATCC 1 cut(s) 1141
BsuRI GGCC 7 cut(s) 469, 535, 815, 1389, 1693, 1772, 1778
BtgI CCRYGG 1 cut(s) 891
BtsCI GGATG 3 cut(s) 492, 1180, 1369
BtsI GCAGTG 2 cut(s) 334, 2143
BtsIMutI CAGTG 8 cut(s) 334, 465, 739, 801, 840, 1382, 1604, 2143
BveI ACCTGC 1 cut(s) 851
CciI TCATGA 1 cut(s) 721
CfoI GCGC 1 cut(s) 257
Cfr10I RCCGGY 4 cut(s) 174, 531, 963, 1871
Cfr13I GGNCC 6 cut(s) 230, 813, 814, 917, 1387, 1692
CseI GACGC 1 cut(s) 134
Csp6I GTAC 3 cut(s) 864, 1077, 1188
CviQI GTAC 3 cut(s) 864, 1077, 1188
DdeI CTNAG 9 cut(s) 592, 615, 1067, 1311, 1394, 1791, 1902, 2091, 2132
DpnI GATC 5 cut(s) 429, 712, 927, 1178, 1513
DpnII GATC 5 cut(s) 427, 710, 925, 1176, 1511
DriI GACNNNNNGTC 1 cut(s) 1099
EaeI YGGCCR 1 cut(s) 1770
Eam1104I CTCTTC 1 cut(s) 1539
Eam1105I GACNNNNNGTC 1 cut(s) 1099
EarI CTCTTC 1 cut(s) 1539
Eco130I CCWWGG 3 cut(s) 891, 913, 1080
Eco24I GRGCYC 2 cut(s) 213, 817
Eco31I GGTCTC 3 cut(s) 1521, 1626, 2094
Eco47I GGWCC 2 cut(s) 230, 917
Eco57I CTGAAG 2 cut(s) 715, 1560
Eco88I CYCGRG 4 cut(s) 205, 471, 810, 2013
Eco91I GGTNACC 1 cut(s) 1874
EcoO109I RGGNCCY 2 cut(s) 814, 1387
EcoO65I GGTNACC 1 cut(s) 1874
EcoRII CCWGG 2 cut(s) 280, 1824
EcoT14I CCWWGG 3 cut(s) 891, 913, 1080
EcoT22I ATGCAT 3 cut(s) 484, 1302, 1369
EcoT38I GRGCYC 2 cut(s) 213, 817
ErhI CCWWGG 3 cut(s) 891, 913, 1080
Esp3I CGTCTC 1 cut(s) 96
FalI AAGNNNNNCTT 2 cut(s) 12, 44
FaqI GGGAC 2 cut(s) 389, 1060
FauI CCCGC 1 cut(s) 968
FauNDI CATATG 1 cut(s) 1945
FblI GTMKAC 5 cut(s) 822, 1092, 1581, 1731, 2073
Fnu4HI GCNGC 8 cut(s) 784, 853, 1004, 1236, 1290, 1758, 1788, 1899
FokI GGATG 3 cut(s) 499, 1167, 1376
FriOI GRGCYC 2 cut(s) 213, 817
Fsp4HI GCNGC 8 cut(s) 784, 853, 1004, 1236, 1290, 1758, 1788, 1899
FspBI CTAG 5 cut(s) 114, 1164, 1590, 1712, 2105
FspI TGCGCA 1 cut(s) 256
GlaI GCGC 1 cut(s) 256
GluI GCNGC 8 cut(s) 784, 853, 1004, 1236, 1290, 1758, 1788, 1899
GsaI CCCAGC 1 cut(s) 1473
GsuI CTGGAG 2 cut(s) 1545, 1847
HaeIII GGCC 7 cut(s) 469, 535, 815, 1389, 1693, 1772, 1778
HapII CCGG 7 cut(s) 175, 214, 532, 964, 996, 1704, 1872
HgaI GACGC 1 cut(s) 134
HhaI GCGC 1 cut(s) 257
Hin1I GRCGYC 1 cut(s) 126
Hin6I GCGC 1 cut(s) 255
HinP1I GCGC 1 cut(s) 255
HincII GTYRAC 1 cut(s) 1582
HindII GTYRAC 1 cut(s) 1582
HindIII AAGCTT 3 cut(s) 26, 589, 869
HinfI GANTC 5 cut(s) 77, 110, 666, 1357, 2021
HpaII CCGG 7 cut(s) 175, 214, 532, 964, 996, 1704, 1872
HphI GGTGA 8 cut(s) 78, 1430, 1573, 1685, 1886, 2030, 2092, 2109
Hpy188III TCNNGA 7 cut(s) 114, 473, 560, 689, 722, 1361, 1977
Hpy99I CGWCG 5 cut(s) 128, 984, 1227, 1941, 2127
HpyAV CCTTC 6 cut(s) 930, 955, 986, 993, 1400, 2051
HpyCH4III ACNGT 7 cut(s) 90, 493, 835, 1159, 1377, 1937, 2088
HpyCH4IV ACGT 3 cut(s) 542, 1968, 2170
HpyF3I CTNAG 9 cut(s) 592, 615, 1067, 1311, 1394, 1791, 1902, 2091, 2132
HpySE526I ACGT 3 cut(s) 542, 1968, 2170
Hsp92I GRCGYC 1 cut(s) 126
HspAI GCGC 1 cut(s) 255
KpnI GGTACC 1 cut(s) 1080
KroI GCCGGC 2 cut(s) 531, 963
KroNI GCCGGC 2 cut(s) 533, 965
Kzo9I GATC 5 cut(s) 427, 710, 925, 1176, 1511
LmnI GCTCC 4 cut(s) 6, 173, 216, 760
Lsp1109I GCAGC 7 cut(s) 795, 864, 1015, 1247, 1276, 1799, 1910
LweI GCATC 8 cut(s) 111, 469, 1276, 1287, 1354, 1489, 1510, 1905
MaeI CTAG 5 cut(s) 114, 1164, 1590, 1712, 2105
MaeII ACGT 3 cut(s) 542, 1968, 2170
MaeIII GTNAC 7 cut(s) 543, 1159, 1561, 1602, 1678, 1874, 2080
MalI GATC 5 cut(s) 429, 712, 927, 1178, 1513
MboI GATC 5 cut(s) 427, 710, 925, 1176, 1511
MboII GAAGA 5 cut(s) 126, 647, 1553, 1556, 1961
MfeI CAATTG 2 cut(s) 655, 1451
MflI RGATCY 2 cut(s) 1176, 1511
MhlI GDGCHC 3 cut(s) 213, 317, 817
MluI ACGCGT 1 cut(s) 517
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 3 cut(s) 147, 179, 195
Mph1103I ATGCAT 3 cut(s) 484, 1302, 1369
MroNI GCCGGC 2 cut(s) 531, 963
MroXI GAANNNNTTC 1 cut(s) 1330
MseI TTAA 6 cut(s) 266, 453, 740, 1907, 2142, 2173
MslI CAYNNNNRTG 2 cut(s) 291, 1993
MspA1I CMGCKG 3 cut(s) 701, 1790, 1901
MspI CCGG 7 cut(s) 175, 214, 532, 964, 996, 1704, 1872
MspR9I CCNGG 2 cut(s) 282, 1826
MunI CAATTG 2 cut(s) 655, 1451
Mva1269I GAATGC 3 cut(s) 1300, 1367, 1801
MvaI CCWGG 2 cut(s) 282, 1826
MvnI CGCG 1 cut(s) 519
NaeI GCCGGC 2 cut(s) 533, 965
NcoI CCATGG 1 cut(s) 891
NdeI CATATG 1 cut(s) 1945
NdeII GATC 5 cut(s) 427, 710, 925, 1176, 1511
NgoMIV GCCGGC 2 cut(s) 531, 963
NmeAIII GCCGAG 2 cut(s) 1084, 1798
NmuCI GTSAC 6 cut(s) 543, 1561, 1602, 1678, 1874, 2080
NsbI TGCGCA 1 cut(s) 256
NsiI ATGCAT 3 cut(s) 484, 1302, 1369
NspI RCATGY 2 cut(s) 296, 1421
PaeI GCATGC 1 cut(s) 296
PaeR7I CTCGAG 2 cut(s) 471, 2013
PagI TCATGA 1 cut(s) 721
PcsI WCGNNNNNNNCGW 2 cut(s) 194, 1692
PctI GAATGC 3 cut(s) 1300, 1367, 1801
PdiI GCCGGC 2 cut(s) 533, 965
PdmI GAANNNNTTC 1 cut(s) 1330
PfeI GAWTC 4 cut(s) 77, 666, 1357, 2021
PflFI GACNNNGTC 1 cut(s) 2086
PfoI TCCNGGA 1 cut(s) 1824
PkrI GCNGC 8 cut(s) 785, 854, 1005, 1237, 1291, 1759, 1789, 1900
PleI GAGTC 1 cut(s) 118
PpsI GAGTC 1 cut(s) 118
PshAI GACNNNNGTC 1 cut(s) 235
Psp6I CCWGG 2 cut(s) 280, 1824
PspEI GGTNACC 1 cut(s) 1874
PspFI CCCAGC 1 cut(s) 1469
PspGI CCWGG 2 cut(s) 280, 1824
PspOMI GGGCCC 1 cut(s) 813
PspPI GGNCC 6 cut(s) 230, 813, 814, 917, 1387, 1692
PspXI VCTCGAGB 1 cut(s) 2013
PstI CTGCAG 2 cut(s) 73, 310
PsuI RGATCY 2 cut(s) 1176, 1511
PsyI GACNNNGTC 1 cut(s) 2086
PvuII CAGCTG 3 cut(s) 701, 1790, 1901
RsaI GTAC 3 cut(s) 865, 1078, 1189
RsaNI GTAC 3 cut(s) 864, 1077, 1188
RseI CAYNNNNRTG 2 cut(s) 291, 1993
SalI GTCGAC 1 cut(s) 1580
SaqAI TTAA 6 cut(s) 266, 453, 740, 1907, 2142, 2173
SatI GCNGC 8 cut(s) 784, 853, 1004, 1236, 1290, 1758, 1788, 1899
Sau3AI GATC 5 cut(s) 427, 710, 925, 1176, 1511
Sau96I GGNCC 6 cut(s) 230, 813, 814, 917, 1387, 1692
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 2 cut(s) 282, 1826
SduI GDGCHC 3 cut(s) 213, 317, 817
SfaNI GCATC 8 cut(s) 111, 469, 1276, 1287, 1354, 1489, 1510, 1905
SfcI CTRYAG 3 cut(s) 69, 306, 954
Sfr274I CTCGAG 2 cut(s) 471, 2013
SinI GGWCC 2 cut(s) 230, 917
SlaI CTCGAG 2 cut(s) 471, 2013
SmiMI CAYNNNNRTG 2 cut(s) 291, 1993
SmlI CTYRAG 3 cut(s) 471, 749, 2013
SmoI CTYRAG 3 cut(s) 471, 749, 2013
SpeI ACTAGT 2 cut(s) 1163, 1711
SphI GCATGC 1 cut(s) 296
SsiI CCGC 4 cut(s) 961, 1096, 1757, 1879
SspI AATATT 1 cut(s) 1573
SspMI CTAG 5 cut(s) 114, 1164, 1590, 1712, 2105
StyD4I CCNGG 2 cut(s) 280, 1824
StyI CCWWGG 3 cut(s) 891, 913, 1080
TaaI ACNGT 7 cut(s) 90, 493, 835, 1159, 1377, 1937, 2088
TaiI ACGT 3 cut(s) 545, 1971, 2173
TaqI TCGA 6 cut(s) 472, 1295, 1581, 1686, 2014, 2122
TauI GCSGC 1 cut(s) 1760
TfiI GAWTC 4 cut(s) 77, 666, 1357, 2021
Tru1I TTAA 6 cut(s) 266, 453, 740, 1907, 2142, 2173
Tru9I TTAA 6 cut(s) 266, 453, 740, 1907, 2142, 2173
TscAI CASTG 8 cut(s) 341, 465, 739, 801, 840, 1382, 1611, 2143
TseFI GTSAC 6 cut(s) 543, 1561, 1602, 1678, 1874, 2080
TseI GCWGC 7 cut(s) 783, 852, 1003, 1235, 1289, 1787, 1898
Tsp45I GTSAC 6 cut(s) 543, 1561, 1602, 1678, 1874, 2080
TspDTI ATGAA 7 cut(s) 290, 600, 734, 1327, 1583, 1680, 1962
TspGWI ACGGA 2 cut(s) 186, 1211
TspRI CASTG 8 cut(s) 341, 465, 739, 801, 840, 1382, 1611, 2143
Tth111I GACNNNGTC 1 cut(s) 2086
VpaK11BI GGWCC 2 cut(s) 230, 917
XapI RAATTY 3 cut(s) 369, 504, 805
XbaI TCTAGA 1 cut(s) 113
XceI RCATGY 2 cut(s) 296, 1421
XcmI CCANNNNNNNNNTGG 1 cut(s) 898
XhoI CTCGAG 2 cut(s) 471, 2013
XmiI GTMKAC 5 cut(s) 822, 1092, 1581, 1731, 2073
XmnI GAANNNNTTC 1 cut(s) 1330
XspI CTAG 5 cut(s) 114, 1164, 1590, 1712, 2105
Zsp2I ATGCAT 3 cut(s) 484, 1302, 1369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.