MD03G1128400.v1.1

histone H2B.1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0019125
Physical Location & Seq
Forward (+)
12487314 .. 12487514
201 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1128400.v1.1.491

Sequence Viewer

Length: 201 bp
ATGGGGATCATGAACAGCTTCATCAACGACATCTTCGAGAAGCTCGCCCAGGAGTCCTCACGGCTCGCGAGGTACAACAAGAAGCCGACGATCACTTCCCGGGAGATACAGACGGCCGTGCGTCTGGTGCTGCCCGGTGAGCTTGCTAAGCACGCCGTCTCTGAGGGGACTAAGGCGGTGACCAAGTTTACTAGCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

67

Amino Acids

7.31

Weight (kDa)

9.87

Isoelectric Point (pI)

31.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 1 - 42 3.1e-10 Core histone H2A/H2B/H3/H4 domain
Histone_ABTB PF26281 8 - 65 3.7e-08 Histone domain in ABTB family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019125)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07790 AT5G22880
malus_domestica MD00G1105600.v1.1 MD03G1128400.v1.1 MD05G1239800.v1.1 MD11G1149100.v1.1
prunus_persica Prupe.6G113500_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0073431

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 68
AciI CCGC 1 cut(s) 176
AclWI GGATC 1 cut(s) 14
AcoI YGGCCR 1 cut(s) 114
AfaI GTAC 1 cut(s) 74
AjnI CCWGG 1 cut(s) 48
AluBI AGCT 4 cut(s) 18, 43, 142, 195
AluI AGCT 4 cut(s) 18, 43, 142, 195
Alw26I GTCTC 1 cut(s) 163
AlwI GGATC 1 cut(s) 14
Ama87I CYCGRG 1 cut(s) 99
AoxI GGCC 1 cut(s) 114
ApeKI GCWGC 1 cut(s) 130
Asp700I GAANNNNTTC 1 cut(s) 17
AsuC2I CCSGG 3 cut(s) 100, 101, 135
AsuHPI GGTGA 2 cut(s) 149, 190
AvaI CYCGRG 1 cut(s) 99
BbvI GCAGC 1 cut(s) 117
BceAI ACGGC 4 cut(s) 77, 101, 129, 140
BciT130I CCWGG 1 cut(s) 50
BcnI CCSGG 3 cut(s) 100, 101, 135
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 1 cut(s) 192
BisI GCNGC 1 cut(s) 131
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 132
Bme1390I CCNGG 4 cut(s) 50, 100, 101, 135
BmeT110I CYCGRG 1 cut(s) 99
BmrFI CCNGG 4 cut(s) 50, 100, 101, 135
Bpu1102I GCTNAGC 1 cut(s) 147
BpuMI CCSGG 3 cut(s) 100, 101, 135
BsaJI CCNNGG 2 cut(s) 48, 99
BseBI CCWGG 1 cut(s) 50
BseDI CCNNGG 2 cut(s) 48, 99
BseMII CTCAG 1 cut(s) 153
BseX3I CGGCCG 1 cut(s) 114
BseXI GCAGC 1 cut(s) 117
Bsh1236I CGCG 1 cut(s) 68
Bsh1285I CGRYCG 1 cut(s) 117
BshFI GGCC 1 cut(s) 116
BsiEI CGRYCG 1 cut(s) 117
BsiHKCI CYCGRG 1 cut(s) 99
BsiSI CCGG 2 cut(s) 100, 135
BslFI GGGAC 1 cut(s) 181
BsmAI GTCTC 1 cut(s) 163
BsmBI CGTCTC 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 181
BsnI GGCC 1 cut(s) 116
BsoBI CYCGRG 1 cut(s) 99
Bsp143I GATC 2 cut(s) 6, 90
Bsp1720I GCTNAGC 1 cut(s) 147
Bsp68I TCGCGA 1 cut(s) 68
BspACI CCGC 1 cut(s) 176
BspANI GGCC 1 cut(s) 116
BspCNI CTCAG 1 cut(s) 154
BspFNI CGCG 1 cut(s) 68
BspHI TCATGA 1 cut(s) 9
BspPI GGATC 1 cut(s) 14
BssECI CCNNGG 2 cut(s) 48, 99
BssMI GATC 2 cut(s) 6, 90
Bst2UI CCWGG 1 cut(s) 50
BstC8I GCNNGC 4 cut(s) 45, 66, 144, 153
BstDEI CTNAG 4 cut(s) 147, 162, 171, 198
BstEII GGTNACC 1 cut(s) 178
BstFNI CGCG 1 cut(s) 68
BstKTI GATC 2 cut(s) 9, 93
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 2 cut(s) 6, 90
BstMCI CGRYCG 1 cut(s) 117
BstMWI GCNNNNNNNGC 4 cut(s) 127, 139, 148, 152
BstNI CCWGG 1 cut(s) 50
BstPI GGTNACC 1 cut(s) 178
BstSCI CCNGG 4 cut(s) 48, 98, 99, 133
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 1 cut(s) 117
BstZI CGGCCG 1 cut(s) 114
BsuRI GGCC 1 cut(s) 116
BtuMI TCGCGA 1 cut(s) 68
Cac8I GCNNGC 4 cut(s) 45, 66, 144, 153
CciI TCATGA 1 cut(s) 9
Cfr9I CCCGGG 1 cut(s) 99
CseI GACGC 1 cut(s) 110
Csp6I GTAC 1 cut(s) 73
CviAII CATG 1 cut(s) 10
CviJI RGCY 7 cut(s) 18, 43, 64, 85, 116, 142, 195
CviKI_1 RGCY 7 cut(s) 18, 43, 64, 85, 116, 142, 195
CviQI GTAC 1 cut(s) 73
DdeI CTNAG 4 cut(s) 147, 162, 171, 198
DpnI GATC 2 cut(s) 8, 92
DpnII GATC 2 cut(s) 6, 90
EaeI YGGCCR 1 cut(s) 114
EagI CGGCCG 1 cut(s) 114
EclXI CGGCCG 1 cut(s) 114
Eco52I CGGCCG 1 cut(s) 114
Eco88I CYCGRG 1 cut(s) 99
Eco91I GGTNACC 1 cut(s) 178
EcoO65I GGTNACC 1 cut(s) 178
EcoRII CCWGG 1 cut(s) 48
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 1 cut(s) 13
FaiI YATR 1 cut(s) 11
FaqI GGGAC 1 cut(s) 181
FatI CATG 1 cut(s) 9
Fnu4HI GCNGC 1 cut(s) 131
Fsp4HI GCNGC 1 cut(s) 131
FspBI CTAG 1 cut(s) 192
GluI GCNGC 1 cut(s) 131
HaeIII GGCC 1 cut(s) 116
HapII CCGG 2 cut(s) 100, 135
HgaI GACGC 1 cut(s) 110
Hin1II CATG 1 cut(s) 13
HinfI GANTC 1 cut(s) 53
HpaII CCGG 2 cut(s) 100, 135
HphI GGTGA 2 cut(s) 149, 190
Hpy166II GTNNAC 1 cut(s) 189
Hpy188I TCNGA 1 cut(s) 163
Hpy188III TCNNGA 3 cut(s) 10, 37, 67
Hpy8I GTNNAC 1 cut(s) 189
Hpy99I CGWCG 1 cut(s) 91
HpyF10VI GCNNNNNNNGC 4 cut(s) 127, 139, 148, 152
HpyF3I CTNAG 4 cut(s) 147, 162, 171, 198
Hsp92II CATG 1 cut(s) 13
Kzo9I GATC 2 cut(s) 6, 90
LpnPI CCDG 5 cut(s) 35, 62, 110, 113, 148
Lsp1109I GCAGC 1 cut(s) 117
MaeI CTAG 1 cut(s) 192
MaeIII GTNAC 1 cut(s) 178
MalI GATC 2 cut(s) 8, 92
MboI GATC 2 cut(s) 6, 90
MboII GAAGA 1 cut(s) 25
MlyI GAGTC 1 cut(s) 62
MnlI CCTC 3 cut(s) 63, 67, 157
MroXI GAANNNNTTC 1 cut(s) 17
MspI CCGG 2 cut(s) 100, 135
MspR9I CCNGG 4 cut(s) 50, 100, 101, 135
MvaI CCWGG 1 cut(s) 50
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 4 cut(s) 127, 139, 148, 152
NciI CCSGG 3 cut(s) 100, 101, 135
NdeII GATC 2 cut(s) 6, 90
NlaIII CATG 1 cut(s) 13
NmuCI GTSAC 1 cut(s) 178
NruI TCGCGA 1 cut(s) 68
PagI TCATGA 1 cut(s) 9
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PdmI GAANNNNTTC 1 cut(s) 17
PkrI GCNGC 1 cut(s) 132
PleI GAGTC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 61
Psp6I CCWGG 1 cut(s) 48
PspEI GGTNACC 1 cut(s) 178
PspGI CCWGG 1 cut(s) 48
RruI TCGCGA 1 cut(s) 68
RsaI GTAC 1 cut(s) 74
RsaNI GTAC 1 cut(s) 73
SatI GCNGC 1 cut(s) 131
Sau3AI GATC 2 cut(s) 6, 90
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 4 cut(s) 50, 100, 101, 135
SetI ASST 5 cut(s) 20, 45, 74, 144, 197
SmaI CCCGGG 1 cut(s) 101
SsiI CCGC 1 cut(s) 176
SspMI CTAG 1 cut(s) 192
StyD4I CCNGG 4 cut(s) 48, 98, 99, 133
TaqI TCGA 1 cut(s) 36
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 1 cut(s) 130
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 2 cut(s) 10, 26
TspMI CCCGGG 1 cut(s) 99
XmaI CCCGGG 1 cut(s) 99
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.