MD11G1149100.v1.1

histone H2B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0019125
Physical Location & Seq
Forward (+)
14121177 .. 14121925
749 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1149100.v1.1.491

Sequence Viewer

Length: 429 bp
ATGGCTCCCAAGGCAGAGAAGAAGCCGGCTGAGAAGAAGCCCGCGGAGGAGAAGAAGGCTGAGAAGGCCCCCGCTGAGAAAAAGCCCCGTGCCGAGAAGAATCTCCCCAAGGAGGCCGCCGGCGAGAAGAAGAAGAAGAGATCGAAGAAGAGCGTGGAGACCTACAAGATCTACATCTTCAAGGTCCTGAAACAGGTCCACCCAGACATCGGAATCTCCAGCAAGGCCATGGGGATCATGAACAGCTTCATCAACGACATCTTTGAGAAGCTCGCTCAGGAGTCGTCGCGGCTCGCGAGGTACAACAAGAAGCCAACGATCACTTCCCGGGAGATTCAGACGGCGGTGAGGTTGGTGCTCCCCGGTGAGCTCGCCAAGCACGCTGTCTCTGAGGGGACTAAGTCGGTGACCAAGTTTACTAGCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

143

Amino Acids

15.85

Weight (kDa)

10.02

Isoelectric Point (pI)

32.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 38 - 118 2.1e-21 Core histone H2A/H2B/H3/H4 domain
Histone_ABTB PF26281 72 - 140 2.6e-07 Histone domain in ABTB family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019125)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07790 AT5G22880
malus_domestica MD00G1105600.v1.1 MD03G1128400.v1.1 MD05G1239800.v1.1 MD11G1149100.v1.1
prunus_persica Prupe.6G113500_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0073431

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 44, 289, 296
AciI CCGC 6 cut(s) 42, 44, 72, 117, 289, 344
AclWI GGATC 1 cut(s) 242
AfaI GTAC 1 cut(s) 302
AfiI CCNNNNNNNGG 3 cut(s) 112, 193, 209
AgsI TTSAA 1 cut(s) 181
AjuI GAANNNNNNNTTGG 2 cut(s) 307, 339
AluBI AGCT 4 cut(s) 246, 271, 370, 423
AluI AGCT 4 cut(s) 246, 271, 370, 423
Alw21I GWGCWC 2 cut(s) 360, 372
Alw26I GTCTC 2 cut(s) 152, 391
AlwI GGATC 1 cut(s) 242
Ama87I CYCGRG 1 cut(s) 327
AoxI GGCC 3 cut(s) 66, 114, 225
Asp700I GAANNNNTTC 1 cut(s) 245
AspS9I GGNCC 3 cut(s) 67, 184, 196
AsuC2I CCSGG 3 cut(s) 328, 329, 363
AsuHPI GGTGA 3 cut(s) 358, 377, 418
AvaI CYCGRG 1 cut(s) 327
AvaII GGWCC 2 cut(s) 184, 196
BanII GRGCYC 1 cut(s) 372
Bbv12I GWGCWC 2 cut(s) 360, 372
BceAI ACGGC 1 cut(s) 357
BcnI CCSGG 3 cut(s) 328, 329, 363
BcoDI GTCTC 2 cut(s) 152, 391
BfaI CTAG 1 cut(s) 420
BglII AGATCT 1 cut(s) 168
BisI GCNGC 2 cut(s) 117, 290
BlsI GCNGC 2 cut(s) 118, 291
Bme1390I CCNGG 3 cut(s) 328, 329, 363
Bme18I GGWCC 2 cut(s) 184, 196
BmeT110I CYCGRG 1 cut(s) 327
BmgT120I GGNCC 3 cut(s) 67, 184, 196
BmiI GGNNCC 2 cut(s) 6, 69
BmrFI CCNGG 3 cut(s) 328, 329, 363
BpmI CTGGAG 1 cut(s) 202
Bpu10I CCTNAGC 1 cut(s) 276
BpuMI CCSGG 3 cut(s) 328, 329, 363
BsaBI GATNNNNATC 1 cut(s) 173
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 6 cut(s) 9, 42, 108, 228, 327, 361
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 193, 209
Bse118I RCCGGY 2 cut(s) 25, 119
Bse8I GATNNNNATC 1 cut(s) 173
BseDI CCNNGG 6 cut(s) 9, 42, 108, 228, 327, 361
BseJI GATNNNNATC 1 cut(s) 173
BseLI CCNNNNNNNGG 3 cut(s) 112, 193, 209
BseMII CTCAG 5 cut(s) 21, 51, 66, 290, 381
BseRI GAGGAG 1 cut(s) 62
Bsh1236I CGCG 3 cut(s) 44, 289, 296
BshFI GGCC 3 cut(s) 68, 116, 227
BsiHKAI GWGCWC 2 cut(s) 360, 372
BsiHKCI CYCGRG 1 cut(s) 327
BsiSI CCGG 4 cut(s) 26, 120, 328, 363
BslFI GGGAC 1 cut(s) 409
BslI CCNNNNNNNGG 3 cut(s) 112, 193, 209
BsmAI GTCTC 2 cut(s) 152, 391
BsmFI GGGAC 1 cut(s) 409
BsnI GGCC 3 cut(s) 68, 116, 227
Bso31I GGTCTC 1 cut(s) 152
BsoBI CYCGRG 1 cut(s) 327
Bsp1286I GDGCHC 2 cut(s) 360, 372
Bsp143I GATC 4 cut(s) 140, 168, 234, 318
Bsp19I CCATGG 1 cut(s) 228
Bsp68I TCGCGA 1 cut(s) 296
BspACI CCGC 6 cut(s) 42, 44, 72, 117, 289, 344
BspANI GGCC 3 cut(s) 68, 116, 227
BspCNI CTCAG 5 cut(s) 22, 52, 67, 289, 382
BspFNI CGCG 3 cut(s) 44, 289, 296
BspHI TCATGA 1 cut(s) 237
BspLI GGNNCC 2 cut(s) 6, 69
BspPI GGATC 1 cut(s) 242
BspQI GCTCTTC 1 cut(s) 143
BspTNI GGTCTC 1 cut(s) 152
BsrFI RCCGGY 2 cut(s) 25, 119
BssAI RCCGGY 2 cut(s) 25, 119
BssECI CCNNGG 6 cut(s) 9, 42, 108, 228, 327, 361
BssMI GATC 4 cut(s) 140, 168, 234, 318
BssT1I CCWWGG 3 cut(s) 9, 108, 228
Bst6I CTCTTC 2 cut(s) 131, 143
BstC8I GCNNGC 7 cut(s) 27, 42, 121, 273, 294, 372, 381
BstDEI CTNAG 6 cut(s) 30, 60, 75, 276, 390, 399
BstDSI CCRYGG 2 cut(s) 42, 228
BstEII GGTNACC 1 cut(s) 406
BstENI CCTNNNNNAGG 1 cut(s) 191
BstFNI CGCG 3 cut(s) 44, 289, 296
BstKTI GATC 4 cut(s) 143, 171, 237, 321
BstMAI GTCTC 2 cut(s) 152, 391
BstMBI GATC 4 cut(s) 140, 168, 234, 318
BstMWI GCNNNNNNNGC 4 cut(s) 11, 65, 376, 380
BstPI GGTNACC 1 cut(s) 406
BstSCI CCNGG 3 cut(s) 326, 327, 361
BstUI CGCG 3 cut(s) 44, 289, 296
BstX2I RGATCY 1 cut(s) 168
BstYI RGATCY 1 cut(s) 168
BsuRI GGCC 3 cut(s) 68, 116, 227
BtgI CCRYGG 2 cut(s) 42, 228
BtuMI TCGCGA 1 cut(s) 296
Cac8I GCNNGC 7 cut(s) 27, 42, 121, 273, 294, 372, 381
CciI TCATGA 1 cut(s) 237
Cfr10I RCCGGY 2 cut(s) 25, 119
Cfr13I GGNCC 3 cut(s) 67, 184, 196
Cfr42I CCGCGG 1 cut(s) 45
Cfr9I CCCGGG 1 cut(s) 327
Csp6I GTAC 1 cut(s) 301
CviAII CATG 2 cut(s) 229, 238
CviQI GTAC 1 cut(s) 301
DdeI CTNAG 6 cut(s) 30, 60, 75, 276, 390, 399
DpnI GATC 4 cut(s) 142, 170, 236, 320
DpnII GATC 4 cut(s) 140, 168, 234, 318
Eam1104I CTCTTC 2 cut(s) 131, 143
EarI CTCTTC 2 cut(s) 131, 143
Ecl136II GAGCTC 1 cut(s) 370
Eco130I CCWWGG 3 cut(s) 9, 108, 228
Eco24I GRGCYC 1 cut(s) 372
Eco31I GGTCTC 1 cut(s) 152
Eco47I GGWCC 2 cut(s) 184, 196
Eco53kI GAGCTC 1 cut(s) 370
Eco88I CYCGRG 1 cut(s) 327
Eco91I GGTNACC 1 cut(s) 406
EcoICRI GAGCTC 1 cut(s) 370
EcoNI CCTNNNNNAGG 1 cut(s) 191
EcoO109I RGGNCCY 2 cut(s) 67, 184
EcoO65I GGTNACC 1 cut(s) 406
EcoT14I CCWWGG 3 cut(s) 9, 108, 228
EcoT38I GRGCYC 1 cut(s) 372
ErhI CCWWGG 3 cut(s) 9, 108, 228
FaeI CATG 2 cut(s) 232, 241
FaiI YATR 2 cut(s) 230, 239
FaqI GGGAC 1 cut(s) 409
FatI CATG 2 cut(s) 228, 237
FauI CCCGC 2 cut(s) 49, 79
Fnu4HI GCNGC 2 cut(s) 117, 290
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 2 cut(s) 117, 290
FspBI CTAG 1 cut(s) 420
GluI GCNGC 2 cut(s) 117, 290
GsuI CTGGAG 1 cut(s) 202
HaeIII GGCC 3 cut(s) 68, 116, 227
HapII CCGG 4 cut(s) 26, 120, 328, 363
Hin1II CATG 2 cut(s) 232, 241
HinfI GANTC 4 cut(s) 100, 213, 281, 334
HpaII CCGG 4 cut(s) 26, 120, 328, 363
HphI GGTGA 3 cut(s) 358, 377, 418
Hpy166II GTNNAC 2 cut(s) 199, 417
Hpy188I TCNGA 3 cut(s) 212, 339, 391
Hpy188III TCNNGA 4 cut(s) 187, 238, 278, 295
Hpy8I GTNNAC 2 cut(s) 199, 417
Hpy99I CGWCG 1 cut(s) 289
HpyAV CCTTC 2 cut(s) 49, 58
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 65, 376, 380
HpyF3I CTNAG 6 cut(s) 30, 60, 75, 276, 390, 399
Hsp92II CATG 2 cut(s) 232, 241
KroI GCCGGC 2 cut(s) 25, 119
KroNI GCCGGC 2 cut(s) 27, 121
KspI CCGCGG 1 cut(s) 45
Kzo9I GATC 4 cut(s) 140, 168, 234, 318
LguI GCTCTTC 1 cut(s) 143
LmnI GCTCC 2 cut(s) 10, 363
LpnPI CCDG 9 cut(s) 39, 133, 179, 200, 216, 232, 263, 341, 376
MaeI CTAG 1 cut(s) 420
MaeIII GTNAC 1 cut(s) 406
MalI GATC 4 cut(s) 142, 170, 236, 320
MboI GATC 4 cut(s) 140, 168, 234, 318
MflI RGATCY 1 cut(s) 168
MhlI GDGCHC 2 cut(s) 360, 372
MlyI GAGTC 1 cut(s) 290
MnlI CCTC 5 cut(s) 40, 106, 291, 342, 385
MreI CGCCGGCG 1 cut(s) 119
MroNI GCCGGC 2 cut(s) 25, 119
MroXI GAANNNNTTC 1 cut(s) 245
MseI TTAA 1 cut(s) 427
MspA1I CMGCKG 2 cut(s) 44, 74
MspI CCGG 4 cut(s) 26, 120, 328, 363
MspR9I CCNGG 3 cut(s) 328, 329, 363
MvnI CGCG 3 cut(s) 44, 289, 296
MwoI GCNNNNNNNGC 4 cut(s) 11, 65, 376, 380
NaeI GCCGGC 2 cut(s) 27, 121
NciI CCSGG 3 cut(s) 328, 329, 363
NcoI CCATGG 1 cut(s) 228
NdeII GATC 4 cut(s) 140, 168, 234, 318
NgoMIV GCCGGC 2 cut(s) 25, 119
NlaIII CATG 2 cut(s) 232, 241
NlaIV GGNNCC 2 cut(s) 6, 69
NmeAIII GCCGAG 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 406
NruI TCGCGA 1 cut(s) 296
PagI TCATGA 1 cut(s) 237
PciSI GCTCTTC 1 cut(s) 143
PcsI WCGNNNNNNNCGW 1 cut(s) 293
PdiI GCCGGC 2 cut(s) 27, 121
PdmI GAANNNNTTC 1 cut(s) 245
PfeI GAWTC 3 cut(s) 100, 213, 334
PflFI GACNNNGTC 1 cut(s) 400
PkrI GCNGC 2 cut(s) 118, 291
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
PpuMI RGGWCCY 1 cut(s) 184
Psp124BI GAGCTC 1 cut(s) 372
Psp5II RGGWCCY 1 cut(s) 184
PspEI GGTNACC 1 cut(s) 406
PspN4I GGNNCC 2 cut(s) 6, 69
PspPI GGNCC 3 cut(s) 67, 184, 196
PspPPI RGGWCCY 1 cut(s) 184
PsuI RGATCY 1 cut(s) 168
PsyI GACNNNGTC 1 cut(s) 400
RruI TCGCGA 1 cut(s) 296
RsaI GTAC 1 cut(s) 302
RsaNI GTAC 1 cut(s) 301
SacI GAGCTC 1 cut(s) 372
SacII CCGCGG 1 cut(s) 45
SapI GCTCTTC 1 cut(s) 143
SaqAI TTAA 1 cut(s) 427
SatI GCNGC 2 cut(s) 117, 290
Sau3AI GATC 4 cut(s) 140, 168, 234, 318
Sau96I GGNCC 3 cut(s) 67, 184, 196
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 3 cut(s) 328, 329, 363
SduI GDGCHC 2 cut(s) 360, 372
SetI ASST 9 cut(s) 164, 186, 198, 248, 273, 302, 353, 372, 425
Sfr303I CCGCGG 1 cut(s) 45
SgrAI CRCCGGYG 1 cut(s) 119
SgrBI CCGCGG 1 cut(s) 45
SinI GGWCC 2 cut(s) 184, 196
SmaI CCCGGG 1 cut(s) 329
SsiI CCGC 6 cut(s) 42, 44, 72, 117, 289, 344
SspMI CTAG 1 cut(s) 420
SstI GAGCTC 1 cut(s) 372
StyD4I CCNGG 3 cut(s) 326, 327, 361
StyI CCWWGG 3 cut(s) 9, 108, 228
TaqI TCGA 1 cut(s) 143
TauI GCSGC 2 cut(s) 119, 292
TfiI GAWTC 3 cut(s) 100, 213, 334
Tru1I TTAA 1 cut(s) 427
Tru9I TTAA 1 cut(s) 427
TseFI GTSAC 1 cut(s) 406
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 2 cut(s) 238, 254
TspMI CCCGGG 1 cut(s) 327
Tth111I GACNNNGTC 1 cut(s) 400
VpaK11BI GGWCC 2 cut(s) 184, 196
XagI CCTNNNNNAGG 1 cut(s) 191
XcmI CCANNNNNNNNNTGG 1 cut(s) 226
XmaI CCCGGG 1 cut(s) 327
XmnI GAANNNNTTC 1 cut(s) 245
XspI CTAG 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.