Prupe.6G113500_v2.0.a1

Histone H2B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0019125
Physical Location & Seq
Forward (+)
8195589 .. 8196428
840 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G113500.1

Sequence Viewer

Length: 435 bp
ATGGCGCCCAAGGCAGAGAAGAAGCCAGCCGAGAAGAAGCCAGCGGAGGAGAAGAAGGCCGAGAAGGCTCCTGCAGAGAAGAAGCCGAGAGCCGAGAAGAAGCTTCCCAAAGAAGCCTCTGGTGCCACTGACAAGAAGAAGAAGAGATCCAAGAAGAGCGTGGAGACCTACAAGATCTACATCTTCAAGGTCCTGAAGCAGGTCCACCCTGACATTGGGATCTCAAGCAAGGCCATGGGGATCATGAACAGCTTCATCAACGATATCTTCGAGAAGCTCGCCCAGGAGTCTTCGAGGCTCGCTAGATACAACAAGAAGCCGACGATCACTTCCAGGGAGATTCAGACCGCTGTGAGATTGGTTTTGCCTGGTGAGCTTGCTAAGCACGCCGTCTCGGAGGGGACCAAGGCGGTGACCAAGTTTACTAGCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

145

Amino Acids

16.02

Weight (kDa)

10.05

Isoelectric Point (pI)

30.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019125)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07790 AT5G22880
malus_domestica MD00G1105600.v1.1 MD03G1128400.v1.1 MD05G1239800.v1.1 MD11G1149100.v1.1
prunus_persica Prupe.6G113500_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0073431

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 190
AccB1I GGYRCC 2 cut(s) 4, 122
AciI CCGC 3 cut(s) 44, 348, 410
AclWI GGATC 3 cut(s) 141, 227, 248
AcuI CTGAAG 1 cut(s) 215
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 199, 215
AgsI TTSAA 1 cut(s) 187
AjnI CCWGG 3 cut(s) 282, 332, 367
AluBI AGCT 5 cut(s) 103, 252, 277, 376, 429
AluI AGCT 5 cut(s) 103, 252, 277, 376, 429
Alw26I GTCTC 2 cut(s) 158, 397
AlwI GGATC 3 cut(s) 141, 227, 248
AoxI GGCC 2 cut(s) 57, 231
Asp700I GAANNNNTTC 1 cut(s) 251
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 3 cut(s) 190, 202, 402
AsuHPI GGTGA 2 cut(s) 383, 424
AvaII GGWCC 3 cut(s) 190, 202, 402
BanI GGYRCC 2 cut(s) 4, 122
BbsI GAAGAC 1 cut(s) 282
BceAI ACGGC 1 cut(s) 374
BciT130I CCWGG 3 cut(s) 284, 334, 369
BcoDI GTCTC 2 cut(s) 158, 397
BfaI CTAG 2 cut(s) 303, 426
BfmI CTRYAG 1 cut(s) 72
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 190
BglI GCCNNNNNGGC 1 cut(s) 65
BglII AGATCT 1 cut(s) 174
BlpI GCTNAGC 1 cut(s) 381
Bme1390I CCNGG 3 cut(s) 284, 334, 369
Bme18I GGWCC 3 cut(s) 190, 202, 402
BmgT120I GGNCC 3 cut(s) 190, 202, 402
BmiI GGNNCC 4 cut(s) 6, 69, 124, 403
BmrFI CCNGG 3 cut(s) 284, 334, 369
BpiI GAAGAC 1 cut(s) 282
Bpu1102I GCTNAGC 1 cut(s) 381
BpuEI CTTGAG 1 cut(s) 208
BsaBI GATNNNNATC 1 cut(s) 179
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 158
BsaJI CCNNGG 5 cut(s) 9, 234, 282, 333, 405
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 215
Bse8I GATNNNNATC 1 cut(s) 179
BseBI CCWGG 3 cut(s) 284, 334, 369
BseDI CCNNGG 5 cut(s) 9, 234, 282, 333, 405
BseJI GATNNNNATC 1 cut(s) 179
BseLI CCNNNNNNNGG 2 cut(s) 199, 215
BseRI GAGGAG 1 cut(s) 62
BshFI GGCC 2 cut(s) 59, 233
BshNI GGYRCC 2 cut(s) 4, 122
BslFI GGGAC 1 cut(s) 415
BslI CCNNNNNNNGG 2 cut(s) 199, 215
BsmAI GTCTC 2 cut(s) 158, 397
BsmBI CGTCTC 1 cut(s) 397
BsmFI GGGAC 1 cut(s) 415
BsnI GGCC 2 cut(s) 59, 233
Bso31I GGTCTC 1 cut(s) 158
Bsp143I GATC 5 cut(s) 146, 174, 219, 240, 324
Bsp1720I GCTNAGC 1 cut(s) 381
Bsp19I CCATGG 1 cut(s) 234
BspACI CCGC 3 cut(s) 44, 348, 410
BspANI GGCC 2 cut(s) 59, 233
BspHI TCATGA 1 cut(s) 243
BspLI GGNNCC 4 cut(s) 6, 69, 124, 403
BspMAI CTGCAG 1 cut(s) 76
BspMI ACCTGC 1 cut(s) 190
BspPI GGATC 3 cut(s) 141, 227, 248
BspQI GCTCTTC 1 cut(s) 149
BspT107I GGYRCC 2 cut(s) 4, 122
BspTNI GGTCTC 1 cut(s) 158
BssECI CCNNGG 5 cut(s) 9, 234, 282, 333, 405
BssMI GATC 5 cut(s) 146, 174, 219, 240, 324
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 9, 234, 405
Bst2UI CCWGG 3 cut(s) 284, 334, 369
Bst6I CTCTTC 2 cut(s) 137, 149
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 6 cut(s) 27, 42, 279, 300, 378, 387
BstDEI CTNAG 2 cut(s) 381, 432
BstDSI CCRYGG 1 cut(s) 234
BstEII GGTNACC 1 cut(s) 412
BstENI CCTNNNNNAGG 1 cut(s) 197
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 5 cut(s) 149, 177, 222, 243, 327
BstMAI GTCTC 2 cut(s) 158, 397
BstMBI GATC 5 cut(s) 146, 174, 219, 240, 324
BstMWI GCNNNNNNNGC 6 cut(s) 11, 65, 122, 373, 382, 386
BstNI CCWGG 3 cut(s) 284, 334, 369
BstPI GGTNACC 1 cut(s) 412
BstSCI CCNGG 3 cut(s) 282, 332, 367
BstSFI CTRYAG 1 cut(s) 72
BstV2I GAAGAC 1 cut(s) 282
BstX2I RGATCY 3 cut(s) 146, 174, 219
BstYI RGATCY 3 cut(s) 146, 174, 219
BsuRI GGCC 2 cut(s) 59, 233
BtgI CCRYGG 1 cut(s) 234
BtsIMutI CAGTG 1 cut(s) 126
BveI ACCTGC 1 cut(s) 190
Cac8I GCNNGC 6 cut(s) 27, 42, 279, 300, 378, 387
CciI TCATGA 1 cut(s) 243
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 3 cut(s) 190, 202, 402
CviAII CATG 2 cut(s) 235, 244
DdeI CTNAG 2 cut(s) 381, 432
DinI GGCGCC 1 cut(s) 6
DpnI GATC 5 cut(s) 148, 176, 221, 242, 326
DpnII GATC 5 cut(s) 146, 174, 219, 240, 324
Eam1104I CTCTTC 2 cut(s) 137, 149
EarI CTCTTC 2 cut(s) 137, 149
Eco130I CCWWGG 3 cut(s) 9, 234, 405
Eco31I GGTCTC 1 cut(s) 158
Eco32I GATATC 1 cut(s) 265
Eco47I GGWCC 3 cut(s) 190, 202, 402
Eco57I CTGAAG 1 cut(s) 215
Eco91I GGTNACC 1 cut(s) 412
EcoNI CCTNNNNNAGG 1 cut(s) 197
EcoO109I RGGNCCY 1 cut(s) 190
EcoO65I GGTNACC 1 cut(s) 412
EcoRII CCWGG 3 cut(s) 282, 332, 367
EcoRV GATATC 1 cut(s) 265
EcoT14I CCWWGG 3 cut(s) 9, 234, 405
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 3 cut(s) 9, 234, 405
Esp3I CGTCTC 1 cut(s) 397
FaeI CATG 2 cut(s) 238, 247
FaiI YATR 2 cut(s) 236, 245
FaqI GGGAC 1 cut(s) 415
FatI CATG 2 cut(s) 234, 243
FspBI CTAG 2 cut(s) 303, 426
GlaI GCGC 1 cut(s) 6
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 59, 233
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 238, 247
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HindIII AAGCTT 1 cut(s) 101
HinfI GANTC 2 cut(s) 287, 340
HphI GGTGA 2 cut(s) 383, 424
Hpy166II GTNNAC 2 cut(s) 205, 423
Hpy188I TCNGA 2 cut(s) 345, 397
Hpy188III TCNNGA 3 cut(s) 193, 244, 271
Hpy8I GTNNAC 2 cut(s) 205, 423
Hpy99I CGWCG 1 cut(s) 325
HpyAV CCTTC 2 cut(s) 49, 58
HpyCH4V TGCA 1 cut(s) 74
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 65, 122, 373, 382, 386
HpyF3I CTNAG 2 cut(s) 381, 432
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 238, 247
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 5 cut(s) 146, 174, 219, 240, 324
LguI GCTCTTC 1 cut(s) 149
LmnI GCTCC 1 cut(s) 73
MaeI CTAG 2 cut(s) 303, 426
MaeIII GTNAC 1 cut(s) 412
MalI GATC 5 cut(s) 148, 176, 221, 242, 326
MboI GATC 5 cut(s) 146, 174, 219, 240, 324
MflI RGATCY 3 cut(s) 146, 174, 219
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 296
MnlI CCTC 4 cut(s) 40, 127, 288, 391
MroXI GAANNNNTTC 1 cut(s) 251
MspA1I CMGCKG 2 cut(s) 44, 350
MspR9I CCNGG 3 cut(s) 284, 334, 369
MvaI CCWGG 3 cut(s) 284, 334, 369
MwoI GCNNNNNNNGC 6 cut(s) 11, 65, 122, 373, 382, 386
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 1 cut(s) 234
NdeII GATC 5 cut(s) 146, 174, 219, 240, 324
NlaIII CATG 2 cut(s) 238, 247
NlaIV GGNNCC 4 cut(s) 6, 69, 124, 403
NmeAIII GCCGAG 4 cut(s) 55, 85, 111, 118
NmuCI GTSAC 1 cut(s) 412
PagI TCATGA 1 cut(s) 243
PciSI GCTCTTC 1 cut(s) 149
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PdmI GAANNNNTTC 1 cut(s) 251
PfeI GAWTC 1 cut(s) 340
PleI GAGTC 1 cut(s) 295
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 295
PpuMI RGGWCCY 1 cut(s) 190
Psp5II RGGWCCY 1 cut(s) 190
Psp6I CCWGG 3 cut(s) 282, 332, 367
PspEI GGTNACC 1 cut(s) 412
PspGI CCWGG 3 cut(s) 282, 332, 367
PspN4I GGNNCC 4 cut(s) 6, 69, 124, 403
PspPI GGNCC 3 cut(s) 190, 202, 402
PspPPI RGGWCCY 1 cut(s) 190
PstI CTGCAG 1 cut(s) 76
PsuI RGATCY 3 cut(s) 146, 174, 219
SapI GCTCTTC 1 cut(s) 149
Sau3AI GATC 5 cut(s) 146, 174, 219, 240, 324
Sau96I GGNCC 3 cut(s) 190, 202, 402
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 3 cut(s) 284, 334, 369
SetI ASST 8 cut(s) 105, 170, 192, 204, 254, 279, 378, 431
SfcI CTRYAG 1 cut(s) 72
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 3 cut(s) 190, 202, 402
SmlI CTYRAG 1 cut(s) 223
SmoI CTYRAG 1 cut(s) 223
SsiI CCGC 3 cut(s) 44, 348, 410
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 2 cut(s) 303, 426
StyD4I CCNGG 3 cut(s) 282, 332, 367
StyI CCWWGG 3 cut(s) 9, 234, 405
TaqI TCGA 2 cut(s) 270, 293
TfiI GAWTC 1 cut(s) 340
TscAI CASTG 1 cut(s) 133
TseFI GTSAC 1 cut(s) 412
Tsp45I GTSAC 1 cut(s) 412
TspDTI ATGAA 2 cut(s) 244, 260
TspRI CASTG 1 cut(s) 133
VpaK11BI GGWCC 3 cut(s) 190, 202, 402
XagI CCTNNNNNAGG 1 cut(s) 197
XcmI CCANNNNNNNNNTGG 2 cut(s) 157, 212
XmnI GAANNNNTTC 1 cut(s) 251
XspI CTAG 2 cut(s) 303, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.