MD05G1239800.v1.1

histone H2B.1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0019125
Physical Location & Seq
Forward (+)
37063329 .. 37063529
201 bp
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UTR
Exon/CDS
Intron
MD05G1239800.v1.1.491

Sequence Viewer

Length: 201 bp
ATGGGAATCATGAACAGCTTCATCAACGACATCTTCGAGAAGCTCGCCCAGGAGTCGTCCAGGCTAGCCAGGTACAACAAGAAGCCCACCATCACTTCCCGGGAGATCCAGACCGCCGTGAGGCTCGTGCTGCCCGGCGAGCTCGCCAAGCACGCCGTTTCGGAAGGGACAAAAGCCGTGACTAAGTTCACCAGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

67

Amino Acids

7.31

Weight (kDa)

9.87

Isoelectric Point (pI)

31.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 1 - 42 3.1e-10 Core histone H2A/H2B/H3/H4 domain
Histone_ABTB PF26281 8 - 65 3.7e-08 Histone domain in ABTB family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019125)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07790 AT5G22880
malus_domestica MD00G1105600.v1.1 MD03G1128400.v1.1 MD05G1239800.v1.1 MD11G1149100.v1.1
prunus_persica Prupe.6G113500_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0073431

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 114
AclWI GGATC 1 cut(s) 100
AfaI GTAC 1 cut(s) 74
AfiI CCNNNNNNNGG 1 cut(s) 120
AjnI CCWGG 3 cut(s) 48, 59, 68
AluBI AGCT 4 cut(s) 18, 43, 142, 195
AluI AGCT 4 cut(s) 18, 43, 142, 195
Alw21I GWGCWC 1 cut(s) 144
AlwI GGATC 1 cut(s) 100
Ama87I CYCGRG 1 cut(s) 99
ApeKI GCWGC 1 cut(s) 130
Asp700I GAANNNNTTC 1 cut(s) 17
AsuC2I CCSGG 3 cut(s) 100, 101, 135
AsuHPI GGTGA 1 cut(s) 181
AsuNHI GCTAGC 1 cut(s) 64
AvaI CYCGRG 1 cut(s) 99
BanII GRGCYC 1 cut(s) 144
BauI CACGAG 1 cut(s) 125
Bbv12I GWGCWC 1 cut(s) 144
BbvI GCAGC 1 cut(s) 117
BccI CCATC 1 cut(s) 98
BceAI ACGGC 3 cut(s) 101, 140, 161
BciT130I CCWGG 3 cut(s) 50, 61, 70
BcnI CCSGG 3 cut(s) 100, 101, 135
BfaI CTAG 1 cut(s) 65
BisI GCNGC 1 cut(s) 131
BlsI GCNGC 1 cut(s) 132
Bme1390I CCNGG 6 cut(s) 50, 61, 70, 100, 101, 135
BmeT110I CYCGRG 1 cut(s) 99
BmrFI CCNGG 6 cut(s) 50, 61, 70, 100, 101, 135
BmtI GCTAGC 1 cut(s) 68
BpuMI CCSGG 3 cut(s) 100, 101, 135
BsaJI CCNNGG 2 cut(s) 48, 99
Bsc4I CCNNNNNNNGG 1 cut(s) 120
BseBI CCWGG 3 cut(s) 50, 61, 70
BseDI CCNNGG 2 cut(s) 48, 99
BseLI CCNNNNNNNGG 1 cut(s) 120
BseXI GCAGC 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 144
BsiHKCI CYCGRG 1 cut(s) 99
BsiSI CCGG 2 cut(s) 100, 135
BslFI GGGAC 1 cut(s) 181
BslI CCNNNNNNNGG 1 cut(s) 120
BsmFI GGGAC 1 cut(s) 181
BsoBI CYCGRG 1 cut(s) 99
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 1 cut(s) 105
BspACI CCGC 1 cut(s) 114
BspHI TCATGA 1 cut(s) 9
BspOI GCTAGC 1 cut(s) 68
BspPI GGATC 1 cut(s) 100
BssECI CCNNGG 2 cut(s) 48, 99
BssMI GATC 1 cut(s) 105
BssSI CACGAG 1 cut(s) 125
Bst2BI CACGAG 1 cut(s) 125
Bst2UI CCWGG 3 cut(s) 50, 61, 70
BstC8I GCNNGC 5 cut(s) 45, 66, 140, 144, 153
BstDEI CTNAG 1 cut(s) 183
BstKTI GATC 1 cut(s) 108
BstMBI GATC 1 cut(s) 105
BstMWI GCNNNNNNNGC 4 cut(s) 130, 139, 148, 152
BstNI CCWGG 3 cut(s) 50, 61, 70
BstSCI CCNGG 6 cut(s) 48, 59, 68, 98, 99, 133
BstV1I GCAGC 1 cut(s) 117
BstX2I RGATCY 1 cut(s) 105
BstYI RGATCY 1 cut(s) 105
Cac8I GCNNGC 5 cut(s) 45, 66, 140, 144, 153
CciI TCATGA 1 cut(s) 9
Cfr9I CCCGGG 1 cut(s) 99
Csp6I GTAC 1 cut(s) 73
CviAII CATG 1 cut(s) 10
CviJI RGCY 9 cut(s) 18, 43, 64, 68, 85, 124, 142, 176, 195
CviKI_1 RGCY 9 cut(s) 18, 43, 64, 68, 85, 124, 142, 176, 195
CviQI GTAC 1 cut(s) 73
DdeI CTNAG 1 cut(s) 183
DpnI GATC 1 cut(s) 107
DpnII GATC 1 cut(s) 105
Ecl136II GAGCTC 1 cut(s) 142
Eco24I GRGCYC 1 cut(s) 144
Eco53kI GAGCTC 1 cut(s) 142
Eco88I CYCGRG 1 cut(s) 99
EcoICRI GAGCTC 1 cut(s) 142
EcoRII CCWGG 3 cut(s) 48, 59, 68
EcoT38I GRGCYC 1 cut(s) 144
FaeI CATG 1 cut(s) 13
FaiI YATR 1 cut(s) 11
FaqI GGGAC 1 cut(s) 181
FatI CATG 1 cut(s) 9
Fnu4HI GCNGC 1 cut(s) 131
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 1 cut(s) 131
FspBI CTAG 1 cut(s) 65
GluI GCNGC 1 cut(s) 131
HapII CCGG 2 cut(s) 100, 135
Hin1II CATG 1 cut(s) 13
HinfI GANTC 2 cut(s) 6, 53
HpaII CCGG 2 cut(s) 100, 135
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 1 cut(s) 189
Hpy188I TCNGA 1 cut(s) 163
Hpy188III TCNNGA 4 cut(s) 10, 37, 109, 198
Hpy8I GTNNAC 1 cut(s) 189
HpyAV CCTTC 1 cut(s) 158
HpyF10VI GCNNNNNNNGC 4 cut(s) 130, 139, 148, 152
HpyF3I CTNAG 1 cut(s) 183
Hsp92II CATG 1 cut(s) 13
Kzo9I GATC 1 cut(s) 105
LpnPI CCDG 9 cut(s) 35, 46, 55, 62, 73, 82, 113, 122, 148
Lsp1109I GCAGC 1 cut(s) 117
MaeI CTAG 1 cut(s) 65
MaeIII GTNAC 1 cut(s) 178
MalI GATC 1 cut(s) 107
MboI GATC 1 cut(s) 105
MboII GAAGA 1 cut(s) 25
MflI RGATCY 1 cut(s) 105
MhlI GDGCHC 1 cut(s) 144
MlyI GAGTC 1 cut(s) 62
MnlI CCTC 1 cut(s) 114
MroXI GAANNNNTTC 1 cut(s) 17
MspI CCGG 2 cut(s) 100, 135
MspR9I CCNGG 6 cut(s) 50, 61, 70, 100, 101, 135
MvaI CCWGG 3 cut(s) 50, 61, 70
MwoI GCNNNNNNNGC 4 cut(s) 130, 139, 148, 152
NciI CCSGG 3 cut(s) 100, 101, 135
NdeII GATC 1 cut(s) 105
NheI GCTAGC 1 cut(s) 64
NlaIII CATG 1 cut(s) 13
NmuCI GTSAC 1 cut(s) 178
PagI TCATGA 1 cut(s) 9
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 1 cut(s) 6
PkrI GCNGC 1 cut(s) 132
PleI GAGTC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 61
Psp124BI GAGCTC 1 cut(s) 144
Psp6I CCWGG 3 cut(s) 48, 59, 68
PspGI CCWGG 3 cut(s) 48, 59, 68
PsuI RGATCY 1 cut(s) 105
RsaI GTAC 1 cut(s) 74
RsaNI GTAC 1 cut(s) 73
SacI GAGCTC 1 cut(s) 144
SatI GCNGC 1 cut(s) 131
Sau3AI GATC 1 cut(s) 105
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 6 cut(s) 50, 61, 70, 100, 101, 135
SduI GDGCHC 1 cut(s) 144
SetI ASST 5 cut(s) 20, 45, 74, 144, 197
SmaI CCCGGG 1 cut(s) 101
SsiI CCGC 1 cut(s) 114
SspMI CTAG 1 cut(s) 65
SstI GAGCTC 1 cut(s) 144
StyD4I CCNGG 6 cut(s) 48, 59, 68, 98, 99, 133
TaqI TCGA 1 cut(s) 36
TfiI GAWTC 1 cut(s) 6
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 1 cut(s) 130
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 2 cut(s) 10, 26
TspMI CCCGGG 1 cut(s) 99
XmaI CCCGGG 1 cut(s) 99
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.