MD03G1143400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006311
Physical Location & Seq
Forward (+)
15376238 .. 15385365
9128 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1143400.v1.1.491

Sequence Viewer

Length: 3765 bp
ATGCTAAATTCTGGGAATAATGTAAACTGCAGTAGTGCAACACTGTCATCAACTATGCCTTCTTTGCCCCAATGTTTGCCATTGGAGCCTATTATGTTAAGTAATCAAAAGTACACCCGCACAGGAGAACTAAGTAGGGTTCTGGGCCTTCCTCTTGGGAGTATAACAGATGACCATTCTCTTGGAGTTGGCCATTCGAAACCTCCACCTCCGGTGGCAACTGAGGAGCTAAAGCACTTCAAAGAAAGTGTGCAAGATGCCTCTAGAAGGGCCAGGGACAGAGCAAAAATGTTGCGTGAATCTATTTTCAAATTGGATAAGTACAAAGAAGCTTTAAGCTCAAAGAAGAGACAGCGTAGTGATCTTTCATCAAGTGAGAGGTCCAATGGAGTAAACATAGTGAAACTGGGAAGTCAGATTTATAAAAATCCTCGTGAAAATATGACTCAAAGATTAGAAGACAGGACCAAGAGTGTTGGGTTCAACAAACGTGTTAGGACGTCAGTGGCTGATGTGCGGGCAGATGTTAGGTCAGCTGCTACCTCAAGGCAGCAGGTAGTCACAGACAAGGATGAAAAAATGCCTCAGGCTGTTAATGCGGCTTCTGTTAGGATTGAAGAAAAGTCCCGTAGATTGCTTGCTCGAGGTGAAGGGTTGGATCAAAAAGTAAAAAAGAAACGTTCGGTAGGAGCAGTGAGTCATAGAATTACAGGTGGCGAACGGGATATACAACGAGCTACGCATCCAAAGCTGAGCGGTGATCTTAAGCTACGCTCTTGTGATGTACAAGGTGTCAGATTAAAAACTTCACTTGGAGTCGGGGGAATCAACAAGTCAGAACCATCTTTTGAGTTGAGTAATCTCAGCACTTGTACAGTACTCAAGAATGATCTGGAAAATGCTCCTGTTCCAAAAGACCGTTCAGTTCTGTTAGAGCAGAGGGTTGTGAAAGGAGACATTAAGCTAAATATTCAGGAGGAGAACCCTGTAGGCAGTCTTAATACTGTGATAAAAGGAAAGGTTTCTAGGGCGCCACGAACTGGTTCCATTATGAATCTAGATTCATCGCCTAATGTTCACTCTCCGTCTGCACCTTGTCAAGGTTGGCAACAGCCTGCTGGTGAAAACAAAGGCAAAGTGGCAAGTGTTATGAGTAATCAAAACCGTGCAATGTCGAGTGGTTCTTCCATACAACCCATGGCTCAGTGGGTTGGTCAGAGACCGCATAAAAACTCTCGCACAAGGAGAACAAGTTTAGTTGCTCCTACACCAAACAATGCTGAGGCTCAGATTTCCTGTCACAGCTCTGCAACTTCTGATTTTAGTGCTAGAACTTCTTCTGTTGGGACCAACGGATCACAACTTACGAGCAGTTTAGATAATCACACCCCGAAATCTAAAAGAGAACTTCAGAACGTTTCATCTCCATTTGGGTTATCTGGAAGTGAAGAATCTGGAGCTAGGGGAAACAAGTTGAAAGATAAGGGAATGGACAGCATTGAAATTTCCTTAGCTGCAGATCAAAAAGTTGGGGCTCACTTATTCCCCTACAAGAAGAATAAGTCACCAACTAAAGAAATTGGGGATAGTGTGCGAAGACAAGGAAGAAGTGGAAGGGGTTCATCTTTGACAAGGCCTGGCAGTCCTCCAACTATGGAGAAAACGGAGAATTTACCAACCACGAAGTCTCTTCAAGGCCTGAAACCTATGTCCGATAAGAATAAAAGTAAAACAGGTCGTCCACCCTCAAAAAAGATTAAAGATCGCAAGGTGTTAACTTGTGTTGGGCCTTCAACATGCAATGGTTCGTCAGATTTCACAGGTGAATCTGATGATGATCATGAAGAACTATTTTTGGCTGCCAATTCTGCTCGTAATGCTAGTAAATTTGCCTGTTCTGGTCCATTTTGGAAGAAAATGGAATTAGTTTTTGGTTCTCTCAGCTCAGAGGACATATCCTACTTGCAGCAGCAGTTAAGTTTTGCAGAGGAGCTTGGTGAGAGTTTGTCTCAGATGTTTAGTGATGAATACAATATTTCGGAAGTTTTGATGCCTAGAGCAGTCCCTAAGCATTCTGGGGAAAGAGAAGGGAGCCATTTTAGTCAAGATTCATTGAAGACTGATGCTTTATGTGAACAACTTGACATGAAAAGGTTGGAAAAGGTCACTCCATTGTACCAAAGAGTTCTTTCTGCTTTGATTGAAGAAGGTGAAAGTGAAGAACTTTACCATCAGAGTGAAGGGAAAAATATACACCTGTGGTGTGCTACTGATGATTCTCATTGTGGTTCATGTAATCAGAATCATATTGATGTTGAACCCAAAGTTTGGGACAGAATGGAATCTGAAGTTGAGTCGAAAGTAGATGTTCCGACTCAGAAATATTGCACGCTGGACAGACTTTCTTGTGATGGAAGCGCTAAAACTTGTACAATTGGTAATGGAAACATGTCCAGTTTTTTACACAGCCAGGAACAGTGGCATGGAGATGATGACCTTTCAGATTCTGATGTGGAGCAACTGCAACCTAGGAAACTAGATACTCCTAGCTTTCCTTCCTCTGACTGCCAATATCAGTTGATGTGCCTGGATGACAGGCTTCTTCTGGAGCTACAGAGTATTGGTTTATGTCCGGAGAGATTGCCTGATCTAACAGAGGGAGAAGACGTGATTAATCAAGATATAATGGGACTCGAGCAAGAGCTGCATCAACAGATTGCGAGGAAGAAGAAAAACTTAGCAAAAATTGATAAAACTATCCAGAGAGAAAGAGCTACAGAAAGATGGAGAACTGAACTAGTTGCAATGGACCAGCTTATTGAGATGGCTTACAGAAAACGATTGGCTTCCCATGGGAATTATGGTTCAAAAAATGCTGTACGTAAGGTTTCAAAACAAGTAGCTTTGGCTTTTCTCAAACGTACACTTTCTAGATGTCGAAAATTTGAAGAAAGGGGAATTAGTTGCTTTAGTGATCCTGAACTACAGAAGGTTATATTCTCTGAACCATCATGCAACAGTGCTGCAAAATCTATTGATTGTATTGGCTCTGGAACTGCTAGTAATACCTGCAATGGAGGTTCCCATCAAGCGGAAGTCAAGAGATCCGGGGCAGCATCCAGTGTGAGTGGGAGATATGATTCCCATAGTGATAATCTCGAAAGGGGTGATAATCTTGAAAGGGGTTCATCAGTGGCTCTTCATGCTGTCATTGACTCGTCTGGTCAAGCTTCTTCGAAGCATGGTTCAGTGTTGAACTTGAACAAGGGGAAGAAGAGGGAAGTGCTGATTACTGATATTGGTGGAAGTGCCTCCTCAATGGTAACTTCAGCTCTTGACGACACTCATCACGAAGTACAGGAAAAGAAAAATGAGAGAGAAAAGGGCCGACATTCAGATAATTTAAGAAATAATTTGCCCAGCGGAGCTGGTCGCACGTCATTGGACTCCTCCGGAAGTGAATTCAAAACAAAAGGAAATTCCAAGCAAAAGAATACTCAGTTACCAAGTCAATCAGTTGCTAATGCCAGCAATACAAGGAGTGGGGTGGGCCCGTCATTACCCAGTAATACTCGTACACCTTCGTCCAAAGAGGCAGATGAACCCGCTGATTTTGCAAACCTGCAACTACCTGAATTAGATTCACTGGAAGAGAATCAAGATCTCAGTACATGGTTGAACTTTGACGAAGATGGCCTGCAAGATCATGATTCCATTGGCCTCGAAATACCAATGGATGATCTCTCAGAGTTGATGCTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ada3

Protein Analysis

1255

Amino Acids

137.53

Weight (kDa)

8.38

Isoelectric Point (pI)

52.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ada3 PF10198 863 - 958 3.2e-06 Histone acetyltransferases subunit 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 423
AatII GACGTC 1 cut(s) 503
Acc36I ACCTGC 3 cut(s) 544, 3077, 3630
AccB1I GGYRCC 1 cut(s) 1030
AccB7I CCANNNNNTGG 2 cut(s) 1040, 2328
AccBSI CCGCTC 1 cut(s) 756
AccIII TCCGGA 2 cut(s) 2632, 3452
AciI CCGC 8 cut(s) 118, 517, 599, 756, 1223, 3092, 3423, 3606
AclI AACGTT 2 cut(s) 679, 1416
AclWI GGATC 4 cut(s) 666, 1363, 2969, 3099
AcoI YGGCCR 1 cut(s) 190
AcsI RAATTY 7 cut(s) 7, 1503, 1669, 1886, 2942, 3461, 3478
AcuI CTGAAG 3 cut(s) 1394, 2367, 3312
AcyI GRCGYC 2 cut(s) 500, 1031
AfeI AGCGCT 1 cut(s) 2419
AfiI CCNNNNNNNGG 5 cut(s) 267, 1040, 1100, 2328, 3091
AflII CTTAAG 1 cut(s) 764
AflIII ACRYGT 2 cut(s) 490, 2448
AhlI ACTAGT 1 cut(s) 2797
AjiI CACGTC 2 cut(s) 2668, 3438
AjnI CCWGG 4 cut(s) 272, 1636, 2469, 2586
AjuI GAANNNNNNNTTGG 4 cut(s) 904, 936, 1914, 1946
AloI GAACNNNNNNTCC 2 cut(s) 2848, 2880
Alw26I GTCTC 5 cut(s) 343, 948, 1213, 1692, 2013
AlwI GGATC 4 cut(s) 666, 1363, 2969, 3099
AlwNI CAGNNNCTG 2 cut(s) 509, 2507
Ama87I CYCGRG 2 cut(s) 642, 2693
Aor13HI TCCGGA 2 cut(s) 2632, 3452
Aor51HI AGCGCT 1 cut(s) 2419
ApaI GGGCCC 1 cut(s) 3554
ApeKI GCWGC 9 cut(s) 536, 550, 1514, 1859, 1966, 1969, 2704, 3023, 3113
ApoI RAATTY 7 cut(s) 7, 1503, 1669, 1886, 2942, 3461, 3478
AseI ATTAAT 1 cut(s) 2673
Asp700I GAANNNNTTC 4 cut(s) 1021, 1042, 1336, 1618
AspA2I CCTAGG 1 cut(s) 2528
AspLEI GCGC 2 cut(s) 1033, 2420
AsuC2I CCSGG 1 cut(s) 3109
AsuHPI GGTGA 8 cut(s) 659, 770, 1133, 1557, 1835, 2009, 2222, 3179
AsuII TTCGAA 2 cut(s) 197, 3236
AvaI CYCGRG 2 cut(s) 642, 2693
AvaII GGWCC 5 cut(s) 381, 465, 1347, 1901, 2809
AvrII CCTAGG 1 cut(s) 2528
AxyI CCTNAGG 1 cut(s) 585
BaeGI GKGCMC 1 cut(s) 3554
BalI TGGCCA 1 cut(s) 192
BanI GGYRCC 1 cut(s) 1030
BanII GRGCYC 2 cut(s) 1537, 3554
BauI CACGAG 1 cut(s) 432
BbsI GAAGAC 4 cut(s) 465, 1603, 2123, 2670
BbvCI CCTCAGC 1 cut(s) 1281
BbvI GCAGC 9 cut(s) 523, 562, 1501, 1846, 1978, 1981, 2691, 3010, 3125
BccI CCATC 8 cut(s) 850, 2238, 2405, 2778, 2818, 3016, 3093, 3686
BciT130I CCWGG 4 cut(s) 274, 1638, 2471, 2588
BclI TGATCA 1 cut(s) 1837
BcnI CCSGG 1 cut(s) 3109
BcoDI GTCTC 5 cut(s) 343, 948, 1213, 1692, 2013
BcuI ACTAGT 1 cut(s) 2797
BfmI CTRYAG 6 cut(s) 28, 987, 1515, 2612, 2775, 2984
BfoI RGCGCY 2 cut(s) 1034, 2421
BfrI CTTAAG 1 cut(s) 764
BfuAI ACCTGC 3 cut(s) 544, 3077, 3630
BglII AGATCT 1 cut(s) 3661
BlnI CCTAGG 1 cut(s) 2528
BlpI GCTNAGC 1 cut(s) 752
BmcAI AGTACT 1 cut(s) 879
Bme1390I CCNGG 5 cut(s) 274, 1638, 2471, 2588, 3109
Bme18I GGWCC 5 cut(s) 381, 465, 1347, 1901, 2809
BmeT110I CYCGRG 2 cut(s) 642, 2693
BmgBI CACGTC 2 cut(s) 2668, 3438
BmiI GGNNCC 7 cut(s) 87, 1032, 1045, 1348, 2093, 3082, 3552
BmrFI CCNGG 5 cut(s) 274, 1638, 2471, 2588, 3109
BmrI ACTGGG 2 cut(s) 416, 3558
BmsI GCATC 7 cut(s) 247, 751, 2040, 2113, 2716, 3125, 3744
BmuI ACTGGG 2 cut(s) 416, 3558
BpiI GAAGAC 4 cut(s) 465, 1603, 2123, 2670
BplI GAGNNNNNCTC 2 cut(s) 1993, 2025
BpmI CTGGAG 2 cut(s) 1476, 2627
Bpu10I CCTNAGC 3 cut(s) 1281, 1510, 2067
Bpu1102I GCTNAGC 1 cut(s) 752
Bpu14I TTCGAA 2 cut(s) 197, 3236
BpuEI CTTGAG 2 cut(s) 529, 866
BpuMI CCSGG 1 cut(s) 3109
BsaAI YACGTR 1 cut(s) 2882
BsaBI GATNNNNATC 1 cut(s) 1836
BsaHI GRCGYC 2 cut(s) 500, 1031
BsaI GGTCTC 1 cut(s) 1213
BsaJI CCNNGG 5 cut(s) 273, 1197, 2528, 2851, 3108
BsaWI WCCGGW 3 cut(s) 211, 2632, 3452
BsaXI ACNNNNNCTCC 4 cut(s) 1237, 1267, 2652, 2682
Bsc4I CCNNNNNNNGG 5 cut(s) 267, 1040, 1100, 2328, 3091
Bse1I ACTGG 6 cut(s) 411, 1045, 2454, 3120, 3564, 3651
Bse21I CCTNAGG 1 cut(s) 585
Bse3DI GCAATG 4 cut(s) 1176, 1807, 2811, 3079
Bse8I GATNNNNATC 1 cut(s) 1836
BseAI TCCGGA 2 cut(s) 2632, 3452
BseBI CCWGG 4 cut(s) 274, 1638, 2471, 2588
BseDI CCNNGG 5 cut(s) 273, 1197, 2528, 2851, 3108
BseGI GGATG 5 cut(s) 577, 742, 2596, 3116, 3742
BseJI GATNNNNATC 1 cut(s) 1836
BseLI CCNNNNNNNGG 5 cut(s) 267, 1040, 1100, 2328, 3091
BseMI GCAATG 4 cut(s) 1176, 1807, 2811, 3079
BseNI ACTGG 6 cut(s) 411, 1045, 2454, 3120, 3564, 3651
BseRI GAGGAG 5 cut(s) 239, 992, 2003, 3304, 3439
BseSI GKGCMC 1 cut(s) 3554
BseXI GCAGC 9 cut(s) 523, 562, 1501, 1846, 1978, 1981, 2691, 3010, 3125
BseYI CCCAGC 1 cut(s) 3419
BsgI GTGCAG 1 cut(s) 1074
BshNI GGYRCC 1 cut(s) 1030
BsiHKCI CYCGRG 2 cut(s) 642, 2693
BsiSI CCGG 4 cut(s) 212, 2633, 3108, 3453
BslFI GGGAC 6 cut(s) 290, 610, 1360, 2048, 2345, 2703
BslI CCNNNNNNNGG 5 cut(s) 267, 1040, 1100, 2328, 3091
BsmAI GTCTC 5 cut(s) 343, 948, 1213, 1692, 2013
BsmFI GGGAC 6 cut(s) 290, 610, 1360, 2048, 2345, 2703
BsmI GAATGC 1 cut(s) 2071
Bso31I GGTCTC 1 cut(s) 1213
BsoBI CYCGRG 2 cut(s) 642, 2693
Bsp119I TTCGAA 2 cut(s) 197, 3236
Bsp120I GGGCCC 1 cut(s) 3550
Bsp1286I GDGCHC 2 cut(s) 1537, 3554
Bsp13I TCCGGA 2 cut(s) 2632, 3452
Bsp1407I TGTACA 3 cut(s) 784, 872, 2429
Bsp1720I GCTNAGC 1 cut(s) 752
Bsp19I CCATGG 2 cut(s) 1197, 2851
BspACI CCGC 8 cut(s) 118, 517, 599, 756, 1223, 3092, 3423, 3606
BspEI TCCGGA 2 cut(s) 2632, 3452
BspHI TCATGA 2 cut(s) 1840, 3706
BspLI GGNNCC 7 cut(s) 87, 1032, 1045, 1348, 2093, 3082, 3552
BspMAI CTGCAG 2 cut(s) 32, 1519
BspMI ACCTGC 3 cut(s) 544, 3077, 3630
BspPI GGATC 4 cut(s) 666, 1363, 2969, 3099
BspQI GCTCTTC 1 cut(s) 3204
BspT104I TTCGAA 2 cut(s) 197, 3236
BspT107I GGYRCC 1 cut(s) 1030
BspTI CTTAAG 1 cut(s) 764
BspTNI GGTCTC 1 cut(s) 1213
BsrBI CCGCTC 1 cut(s) 756
BsrDI GCAATG 4 cut(s) 1176, 1807, 2811, 3079
BsrGI TGTACA 3 cut(s) 784, 872, 2429
BsrI ACTGG 6 cut(s) 411, 1045, 2454, 3120, 3564, 3651
BssECI CCNNGG 5 cut(s) 273, 1197, 2528, 2851, 3108
BssNI GRCGYC 2 cut(s) 500, 1031
BssSI CACGAG 1 cut(s) 432
BssT1I CCWWGG 3 cut(s) 1197, 2528, 2851
Bst2BI CACGAG 1 cut(s) 432
Bst2UI CCWGG 4 cut(s) 274, 1638, 2471, 2588
Bst4CI ACNGT 7 cut(s) 45, 877, 920, 1006, 1166, 2478, 3020
Bst6I CTCTTC 5 cut(s) 341, 1695, 3204, 3269, 3645
BstACI GRCGYC 2 cut(s) 500, 1031
BstAFI CTTAAG 1 cut(s) 764
BstAPI GCANNNNNTGC 1 cut(s) 2704
BstAUI TGTACA 3 cut(s) 784, 872, 2429
BstBAI YACGTR 1 cut(s) 2882
BstBI TTCGAA 2 cut(s) 197, 3236
BstC8I GCNNGC 6 cut(s) 519, 639, 1116, 2390, 3529, 3698
BstDSI CCRYGG 2 cut(s) 1197, 2851
BstENI CCTNNNNNAGG 2 cut(s) 265, 1098
BstF5I GGATG 5 cut(s) 577, 742, 2596, 3116, 3742
BstH2I RGCGCY 2 cut(s) 1034, 2421
BstHHI GCGC 2 cut(s) 1033, 2420
BstMAI GTCTC 5 cut(s) 343, 948, 1213, 1692, 2013
BstMWI GCNNNNNNNGC 9 cut(s) 64, 85, 596, 748, 1868, 1877, 2704, 3203, 3614
BstNI CCWGG 4 cut(s) 274, 1638, 2471, 2588
BstNSI RCATGY 2 cut(s) 1800, 2452
BstSCI CCNGG 5 cut(s) 272, 1636, 2469, 2586, 3107
BstSFI CTRYAG 6 cut(s) 28, 987, 1515, 2612, 2775, 2984
BstSLI GKGCMC 1 cut(s) 3554
BstSNI TACGTA 1 cut(s) 2882
BstV1I GCAGC 9 cut(s) 523, 562, 1501, 1846, 1978, 1981, 2691, 3010, 3125
BstV2I GAAGAC 4 cut(s) 465, 1603, 2123, 2670
BstX2I RGATCY 2 cut(s) 3104, 3661
BstXI CCANNNNNNTGG 1 cut(s) 182
BstYI RGATCY 2 cut(s) 3104, 3661
Bsu36I CCTNAGG 1 cut(s) 585
BtgI CCRYGG 2 cut(s) 1197, 2851
BtgZI GCGATG 1 cut(s) 1050
BtrI CACGTC 2 cut(s) 2668, 3438
BtsCI GGATG 5 cut(s) 577, 742, 2596, 3116, 3742
BtsI GCAGTG 1 cut(s) 699
BveI ACCTGC 3 cut(s) 544, 3077, 3630
Cac8I GCNNGC 6 cut(s) 519, 639, 1116, 2390, 3529, 3698
CaiI CAGNNNCTG 2 cut(s) 509, 2507
CciI TCATGA 2 cut(s) 1840, 3706
CfoI GCGC 2 cut(s) 1033, 2420
DinI GGCGCC 1 cut(s) 1032
EaeI YGGCCR 1 cut(s) 190
Eam1104I CTCTTC 5 cut(s) 341, 1695, 3204, 3269, 3645
EarI CTCTTC 5 cut(s) 341, 1695, 3204, 3269, 3645
Eco105I TACGTA 1 cut(s) 2882
Eco130I CCWWGG 3 cut(s) 1197, 2528, 2851
Eco147I AGGCCT 2 cut(s) 1636, 1698
Eco24I GRGCYC 2 cut(s) 1537, 3554
Eco31I GGTCTC 1 cut(s) 1213
Eco47I GGWCC 5 cut(s) 381, 465, 1347, 1901, 2809
Eco47III AGCGCT 1 cut(s) 2419
Eco57I CTGAAG 3 cut(s) 1394, 2367, 3312
Eco81I CCTNAGG 1 cut(s) 585
Eco88I CYCGRG 2 cut(s) 642, 2693
EcoNI CCTNNNNNAGG 2 cut(s) 265, 1098
EcoRI GAATTC 1 cut(s) 3461
EcoRII CCWGG 4 cut(s) 272, 1636, 2469, 2586
EcoT14I CCWWGG 3 cut(s) 1197, 2528, 2851
EcoT38I GRGCYC 2 cut(s) 1537, 3554
EgeI GGCGCC 1 cut(s) 1032
EheI GGCGCC 1 cut(s) 1032
ErhI CCWWGG 3 cut(s) 1197, 2528, 2851
FalI AAGNNNNNCTT 2 cut(s) 2720, 2752
FaqI GGGAC 6 cut(s) 290, 610, 1360, 2048, 2345, 2703
FauI CCCGC 3 cut(s) 125, 510, 3613
FbaI TGATCA 1 cut(s) 1837
FokI GGATG 5 cut(s) 584, 729, 2603, 3103, 3749
FriOI GRGCYC 2 cut(s) 1537, 3554
GlaI GCGC 2 cut(s) 1032, 2419
GsaI CCCAGC 1 cut(s) 3423
GsuI CTGGAG 2 cut(s) 1476, 2627
HaeII RGCGCY 2 cut(s) 1034, 2421
HapII CCGG 4 cut(s) 212, 2633, 3108, 3453
HhaI GCGC 2 cut(s) 1033, 2420
Hin1I GRCGYC 2 cut(s) 500, 1031
Hin6I GCGC 2 cut(s) 1031, 2418
HinP1I GCGC 2 cut(s) 1031, 2418
HincII GTYRAC 1 cut(s) 1776
HindII GTYRAC 1 cut(s) 1776
HindIII AAGCTT 2 cut(s) 330, 3228
HpaI GTTAAC 1 cut(s) 1776
HpaII CCGG 4 cut(s) 212, 2633, 3108, 3453
HphI GGTGA 8 cut(s) 659, 770, 1133, 1557, 1835, 2009, 2222, 3179
Hpy166II GTNNAC 9 cut(s) 25, 114, 394, 1078, 1742, 1776, 2135, 2924, 3578
Hpy8I GTNNAC 9 cut(s) 25, 114, 394, 1078, 1742, 1776, 2135, 2924, 3578
HpyCH4III ACNGT 7 cut(s) 45, 877, 920, 1006, 1166, 2478, 3020
HpyCH4IV ACGT 8 cut(s) 490, 500, 679, 1416, 2667, 2881, 2920, 3437
HpyF10VI GCNNNNNNNGC 9 cut(s) 64, 85, 596, 748, 1868, 1877, 2704, 3203, 3614
HpySE526I ACGT 8 cut(s) 490, 500, 679, 1416, 2667, 2881, 2920, 3437
Hsp92I GRCGYC 2 cut(s) 500, 1031
HspAI GCGC 2 cut(s) 1031, 2418
KasI GGCGCC 1 cut(s) 1030
Kpn2I TCCGGA 2 cut(s) 2632, 3452
Ksp22I TGATCA 1 cut(s) 1837
KspAI GTTAAC 1 cut(s) 1776
LguI GCTCTTC 1 cut(s) 3204
Lsp1109I GCAGC 9 cut(s) 523, 562, 1501, 1846, 1978, 1981, 2691, 3010, 3125
LweI GCATC 7 cut(s) 247, 751, 2040, 2113, 2716, 3125, 3744
MaeII ACGT 8 cut(s) 490, 500, 679, 1416, 2667, 2881, 2920, 3437
MaeIII GTNAC 6 cut(s) 559, 1298, 1563, 2164, 3322, 3501
MbiI CCGCTC 1 cut(s) 756
MfeI CAATTG 1 cut(s) 2433
MflI RGATCY 2 cut(s) 3104, 3661
MhlI GDGCHC 2 cut(s) 1537, 3554
MlsI TGGCCA 1 cut(s) 192
MluNI TGGCCA 1 cut(s) 192
Mly113I GGCGCC 1 cut(s) 1031
MlyI GAGTC 8 cut(s) 439, 706, 825, 2363, 2368, 2685, 3209, 3440
MmeI TCCRAC 4 cut(s) 636, 1673, 2136, 2396
Mox20I TGGCCA 1 cut(s) 192
MroI TCCGGA 2 cut(s) 2632, 3452
MroXI GAANNNNTTC 4 cut(s) 1021, 1042, 1336, 1618
MscI TGGCCA 1 cut(s) 192
MslI CAYNNNNRTG 3 cut(s) 2235, 2310, 2487
Msp20I TGGCCA 1 cut(s) 192
MspA1I CMGCKG 3 cut(s) 536, 3423, 3608
MspCI CTTAAG 1 cut(s) 764
MspI CCGG 4 cut(s) 212, 2633, 3108, 3453
MspR9I CCNGG 5 cut(s) 274, 1638, 2471, 2588, 3109
MunI CAATTG 1 cut(s) 2433
Mva1269I GAATGC 1 cut(s) 2071
MvaI CCWGG 4 cut(s) 274, 1638, 2471, 2588
MwoI GCNNNNNNNGC 9 cut(s) 64, 85, 596, 748, 1868, 1877, 2704, 3203, 3614
NarI GGCGCC 1 cut(s) 1031
NciI CCSGG 1 cut(s) 3109
NcoI CCATGG 2 cut(s) 1197, 2851
NlaIV GGNNCC 7 cut(s) 87, 1032, 1045, 1348, 2093, 3082, 3552
NmuCI GTSAC 4 cut(s) 559, 1298, 1563, 2164
NspI RCATGY 2 cut(s) 1800, 2452
NspV TTCGAA 2 cut(s) 197, 3236
PaeR7I CTCGAG 2 cut(s) 642, 2693
PagI TCATGA 2 cut(s) 1840, 3706
PceI AGGCCT 2 cut(s) 1636, 1698
PciI ACATGT 1 cut(s) 2448
PciSI GCTCTTC 1 cut(s) 3204
PctI GAATGC 1 cut(s) 2071
PdmI GAANNNNTTC 4 cut(s) 1021, 1042, 1336, 1618
PflMI CCANNNNNTGG 2 cut(s) 1040, 2328
PleI GAGTC 8 cut(s) 439, 705, 824, 2362, 2368, 2685, 3209, 3440
PluTI GGCGCC 1 cut(s) 1034
PpsI GAGTC 8 cut(s) 439, 705, 824, 2362, 2368, 2685, 3209, 3440
Ppu21I YACGTR 1 cut(s) 2882
PscI ACATGT 1 cut(s) 2448
PshBI ATTAAT 1 cut(s) 2673
PsiI TTATAA 1 cut(s) 423
Psp1406I AACGTT 2 cut(s) 679, 1416
Psp6I CCWGG 4 cut(s) 272, 1636, 2469, 2586
PspFI CCCAGC 1 cut(s) 3419
PspGI CCWGG 4 cut(s) 272, 1636, 2469, 2586
PspN4I GGNNCC 7 cut(s) 87, 1032, 1045, 1348, 2093, 3082, 3552
PspOMI GGGCCC 1 cut(s) 3550
PspXI VCTCGAGB 2 cut(s) 642, 2693
PsrI GAACNNNNNNTAC 2 cut(s) 711, 743
PstI CTGCAG 2 cut(s) 32, 1519
PstNI CAGNNNCTG 2 cut(s) 509, 2507
PsuI RGATCY 2 cut(s) 3104, 3661
PvuII CAGCTG 1 cut(s) 536
RseI CAYNNNNRTG 3 cut(s) 2235, 2310, 2487
SapI GCTCTTC 1 cut(s) 3204
ScaI AGTACT 1 cut(s) 879
SchI GAGTC 8 cut(s) 439, 706, 825, 2363, 2368, 2685, 3209, 3440
ScrFI CCNGG 5 cut(s) 274, 1638, 2471, 2588, 3109
SduI GDGCHC 2 cut(s) 1537, 3554
SfaNI GCATC 7 cut(s) 247, 751, 2040, 2113, 2716, 3125, 3744
SfcI CTRYAG 6 cut(s) 28, 987, 1515, 2612, 2775, 2984
SfoI GGCGCC 1 cut(s) 1032
Sfr274I CTCGAG 2 cut(s) 642, 2693
SfuI TTCGAA 2 cut(s) 197, 3236
SinI GGWCC 5 cut(s) 381, 465, 1347, 1901, 2809
SlaI CTCGAG 2 cut(s) 642, 2693
SmiMI CAYNNNNRTG 3 cut(s) 2235, 2310, 2487
SmlI CTYRAG 5 cut(s) 544, 642, 764, 881, 2693
SmoI CTYRAG 5 cut(s) 544, 642, 764, 881, 2693
SnaBI TACGTA 1 cut(s) 2882
SpeI ACTAGT 1 cut(s) 2797
SseBI AGGCCT 2 cut(s) 1636, 1698
SsiI CCGC 8 cut(s) 118, 517, 599, 756, 1223, 3092, 3423, 3606
SspDI GGCGCC 1 cut(s) 1030
SspI AATATT 3 cut(s) 970, 2035, 2384
StuI AGGCCT 2 cut(s) 1636, 1698
StyD4I CCNGG 5 cut(s) 272, 1636, 2469, 2586, 3107
StyI CCWWGG 3 cut(s) 1197, 2528, 2851
TaaI ACNGT 7 cut(s) 45, 877, 920, 1006, 1166, 2478, 3020
TaiI ACGT 8 cut(s) 493, 503, 682, 1419, 2670, 2884, 2923, 3440
TaqI TCGA 9 cut(s) 197, 643, 1175, 2357, 2694, 2938, 3159, 3236, 3723
TatI WGTACW 8 cut(s) 111, 321, 784, 872, 877, 2429, 3355, 3668
TauI GCSGC 1 cut(s) 602
TseFI GTSAC 4 cut(s) 559, 1298, 1563, 2164
TseI GCWGC 9 cut(s) 536, 550, 1514, 1859, 1966, 1969, 2704, 3023, 3113
Tsp45I GTSAC 4 cut(s) 559, 1298, 1563, 2164
TspGWI ACGGA 3 cut(s) 1074, 1368, 1679
Van91I CCANNNNNTGG 2 cut(s) 1040, 2328
Vha464I CTTAAG 1 cut(s) 764
VpaK11BI GGWCC 5 cut(s) 381, 465, 1347, 1901, 2809
VspI ATTAAT 1 cut(s) 2673
XagI CCTNNNNNAGG 2 cut(s) 265, 1098
XapI RAATTY 7 cut(s) 7, 1503, 1669, 1886, 2942, 3461, 3478
XbaI TCTAGA 3 cut(s) 263, 1057, 2930
XceI RCATGY 2 cut(s) 1800, 2452
XcmI CCANNNNNNNNNTGG 2 cut(s) 1195, 2858
XhoI CTCGAG 2 cut(s) 642, 2693
XmaJI CCTAGG 1 cut(s) 2528
XmnI GAANNNNTTC 4 cut(s) 1021, 1042, 1336, 1618
ZraI GACGTC 1 cut(s) 501
ZrmI AGTACT 1 cut(s) 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.