MD03G1162500.v1.1
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013425
Physical Location & Seq
Reverse (-)
19681588 .. 19690659
9072 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1162500.v1.1.491

Sequence Viewer

Length: 561 bp
ATGATGATGGGTTTCATGATGTGGATGGCTAGGAGCACGGTGCATTTGTTTAGCATTGGAATCACCTTATCATCAAATGTGAGTGACTTTTCCTTTTCAAACATACATATGAGCACAAGATGCTATGATCAATCCTGGTGGGGAAGAGCCGAGCCAATCTACGTGACCACATGTCCTCGACACTCAAAGTCAAAGGAGGGTTCAATCTCTAACCTACTCTTCGTTAATATCACCTCAACTTCCGAGAATGGAGTATTCTTATCAGGTTCTAAAGGCGGGCTTCTTAGTGATTTACGATTCATTAACTTGGATCTGACTTACAGAAGATGGACCAACAATGATGGTGGGTTGGTAGACTACAGACCGGGATGCCAGGGACTTGTTAAACACAGCACGGCCGGGATCATCATGGAACACATCAATGGCTTGGTGATTGACAATGTTAATATGAGATGGTCCGATGATCCGTTAAGGCAGTGGAATGATTCTTTGGATTTCAGGTCTACTACTGTAAATAACATATCTCTGCTTAATTTCCACTCCAGCTTGTACAAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

187

Amino Acids

21.13

Weight (kDa)

7.79

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013425)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57790 AT3G57790
fragaria_vesca FvH4_6g22970
malus_domestica MD03G1162500.v1.1 MD12G1064100.v1.1
prunus_persica Prupe.7G070000_v2.0.a1
pyrus_communis pycom12g05590
rosa_chinensis RchiOBHm_Chr3g0480761
rosa_laevigata RLG00000023454
rosa_multiflora Rmu_co8289587.1_g000001 Rmu_sc0003902.1_g000012
rosa_roxburghii Rroxscaffold_6G00401090
rosa_rugosa Rorug03G0185600
rosa_samantha Rh3AG237700 Rh3BG271100 Rh3CG267200 Rh3DG263200
rosa_wichuraiana Rw3G021290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 354, 503
AciI CCGC 1 cut(s) 276
AclWI GGATC 3 cut(s) 318, 410, 458
AcoI YGGCCR 1 cut(s) 396
AfaI GTAC 1 cut(s) 551
AflIII ACRYGT 1 cut(s) 170
AgsI TTSAA 2 cut(s) 99, 204
AhdI GACNNNNNGTC 1 cut(s) 171
AjnI CCWGG 2 cut(s) 134, 372
AjuI GAANNNNNNNTTGG 2 cut(s) 473, 505
AluBI AGCT 1 cut(s) 546
AluI AGCT 1 cut(s) 546
Alw21I GWGCWC 2 cut(s) 38, 116
AlwI GGATC 3 cut(s) 318, 410, 458
AoxI GGCC 1 cut(s) 396
AspS9I GGNCC 2 cut(s) 330, 456
AsuC2I CCSGG 2 cut(s) 366, 400
AsuHPI GGTGA 3 cut(s) 55, 223, 442
AvaII GGWCC 2 cut(s) 330, 456
Bbv12I GWGCWC 2 cut(s) 38, 116
BccI CCATC 4 cut(s) 19, 321, 335, 447
BceAI ACGGC 1 cut(s) 411
BciT130I CCWGG 2 cut(s) 136, 374
BclI TGATCA 1 cut(s) 127
BcnI CCSGG 2 cut(s) 366, 400
BfaI CTAG 1 cut(s) 30
BfmI CTRYAG 1 cut(s) 358
Bme1390I CCNGG 4 cut(s) 136, 366, 374, 400
Bme18I GGWCC 2 cut(s) 330, 456
BmeRI GACNNNNNGTC 1 cut(s) 171
BmgT120I GGNCC 2 cut(s) 330, 456
BmrFI CCNGG 4 cut(s) 136, 366, 374, 400
BmsI GCATC 2 cut(s) 110, 359
BpmI CTGGAG 1 cut(s) 526
BpuMI CCSGG 2 cut(s) 366, 400
BsaAI YACGTR 1 cut(s) 163
BsaJI CCNNGG 1 cut(s) 373
BseBI CCWGG 2 cut(s) 136, 374
BseDI CCNNGG 1 cut(s) 373
BseGI GGATG 2 cut(s) 30, 374
BseX3I CGGCCG 1 cut(s) 396
Bsh1285I CGRYCG 1 cut(s) 399
BshFI GGCC 1 cut(s) 398
BsiEI CGRYCG 1 cut(s) 399
BsiHKAI GWGCWC 2 cut(s) 38, 116
BsiSI CCGG 2 cut(s) 365, 399
BslFI GGGAC 1 cut(s) 390
BsmFI GGGAC 1 cut(s) 390
BsnI GGCC 1 cut(s) 398
Bsp1286I GDGCHC 2 cut(s) 38, 116
Bsp1407I TGTACA 1 cut(s) 549
Bsp143I GATC 4 cut(s) 127, 310, 402, 463
BspACI CCGC 1 cut(s) 276
BspANI GGCC 1 cut(s) 398
BspHI TCATGA 1 cut(s) 15
BspPI GGATC 3 cut(s) 318, 410, 458
BspQI GCTCTTC 1 cut(s) 139
BsrGI TGTACA 1 cut(s) 549
BssECI CCNNGG 1 cut(s) 373
BssMI GATC 4 cut(s) 127, 310, 402, 463
Bst2UI CCWGG 2 cut(s) 136, 374
Bst4CI ACNGT 2 cut(s) 40, 511
Bst6I CTCTTC 2 cut(s) 139, 224
BstAPI GCANNNNNTGC 1 cut(s) 120
BstAUI TGTACA 1 cut(s) 549
BstBAI YACGTR 1 cut(s) 163
BstC8I GCNNGC 1 cut(s) 278
BstDEI CTNAG 1 cut(s) 284
BstF5I GGATG 2 cut(s) 30, 374
BstKTI GATC 4 cut(s) 130, 313, 405, 466
BstMBI GATC 4 cut(s) 127, 310, 402, 463
BstMCI CGRYCG 1 cut(s) 399
BstMWI GCNNNNNNNGC 1 cut(s) 120
BstNI CCWGG 2 cut(s) 136, 374
BstNSI RCATGY 1 cut(s) 174
BstSCI CCNGG 4 cut(s) 134, 364, 372, 398
BstSFI CTRYAG 1 cut(s) 358
BstX2I RGATCY 1 cut(s) 310
BstYI RGATCY 1 cut(s) 310
BstZI CGGCCG 1 cut(s) 396
BsuRI GGCC 1 cut(s) 398
BtsCI GGATG 2 cut(s) 30, 374
BtsI GCAGTG 1 cut(s) 482
BtsIMutI CAGTG 1 cut(s) 482
Cac8I GCNNGC 1 cut(s) 278
CciI TCATGA 1 cut(s) 15
Cfr13I GGNCC 2 cut(s) 330, 456
Csp6I GTAC 1 cut(s) 550
CspCI CAANNNNNGTGG 4 cut(s) 119, 154, 325, 360
CviAII CATG 3 cut(s) 16, 171, 409
CviJI RGCY 7 cut(s) 29, 149, 154, 280, 398, 426, 546
CviKI_1 RGCY 7 cut(s) 29, 149, 154, 280, 398, 426, 546
CviQI GTAC 1 cut(s) 550
DdeI CTNAG 1 cut(s) 284
DpnI GATC 4 cut(s) 129, 312, 404, 465
DpnII GATC 4 cut(s) 127, 310, 402, 463
DriI GACNNNNNGTC 1 cut(s) 171
EaeI YGGCCR 1 cut(s) 396
EagI CGGCCG 1 cut(s) 396
Eam1104I CTCTTC 2 cut(s) 139, 224
Eam1105I GACNNNNNGTC 1 cut(s) 171
EarI CTCTTC 2 cut(s) 139, 224
EclXI CGGCCG 1 cut(s) 396
Eco47I GGWCC 2 cut(s) 330, 456
Eco52I CGGCCG 1 cut(s) 396
EcoRII CCWGG 2 cut(s) 134, 372
FaeI CATG 3 cut(s) 19, 174, 412
FaiI YATR 9 cut(s) 17, 104, 108, 110, 126, 172, 410, 449, 521
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FaqI GGGAC 1 cut(s) 390
FatI CATG 3 cut(s) 15, 170, 408
FauI CCCGC 1 cut(s) 269
FauNDI CATATG 1 cut(s) 108
FbaI TGATCA 1 cut(s) 127
FblI GTMKAC 2 cut(s) 354, 503
FokI GGATG 2 cut(s) 37, 381
FspBI CTAG 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 526
HaeIII GGCC 1 cut(s) 398
HapII CCGG 2 cut(s) 365, 399
Hin1II CATG 3 cut(s) 19, 174, 412
HinfI GANTC 3 cut(s) 60, 297, 485
HpaII CCGG 2 cut(s) 365, 399
HphI GGTGA 3 cut(s) 55, 223, 442
Hpy166II GTNNAC 2 cut(s) 355, 504
Hpy188I TCNGA 3 cut(s) 244, 315, 460
Hpy188III TCNNGA 1 cut(s) 16
Hpy8I GTNNAC 2 cut(s) 355, 504
HpyCH4III ACNGT 2 cut(s) 40, 511
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 1 cut(s) 43
HpyF10VI GCNNNNNNNGC 1 cut(s) 120
HpyF3I CTNAG 1 cut(s) 284
HpySE526I ACGT 1 cut(s) 162
Hsp92II CATG 3 cut(s) 19, 174, 412
Ksp22I TGATCA 1 cut(s) 127
Kzo9I GATC 4 cut(s) 127, 310, 402, 463
LguI GCTCTTC 1 cut(s) 139
LmnI GCTCC 1 cut(s) 33
LpnPI CCDG 9 cut(s) 121, 148, 249, 359, 378, 386, 412, 484, 556
LweI GCATC 2 cut(s) 110, 359
MaeI CTAG 1 cut(s) 30
MaeII ACGT 1 cut(s) 162
MaeIII GTNAC 2 cut(s) 83, 163
MalI GATC 4 cut(s) 129, 312, 404, 465
MboI GATC 4 cut(s) 127, 310, 402, 463
MboII GAAGA 3 cut(s) 156, 211, 336
MflI RGATCY 1 cut(s) 310
MhlI GDGCHC 2 cut(s) 38, 116
MluCI AATT 1 cut(s) 532
MnlI CCTC 3 cut(s) 186, 190, 244
MseI TTAA 6 cut(s) 225, 303, 384, 444, 470, 531
MslI CAYNNNNRTG 2 cut(s) 107, 420
MspI CCGG 2 cut(s) 365, 399
MspR9I CCNGG 4 cut(s) 136, 366, 374, 400
MvaI CCWGG 2 cut(s) 136, 374
MwoI GCNNNNNNNGC 1 cut(s) 120
NciI CCSGG 2 cut(s) 366, 400
NdeI CATATG 1 cut(s) 108
NdeII GATC 4 cut(s) 127, 310, 402, 463
NlaIII CATG 3 cut(s) 19, 174, 412
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 2 cut(s) 83, 163
NspI RCATGY 1 cut(s) 174
PagI TCATGA 1 cut(s) 15
PciI ACATGT 1 cut(s) 170
PciSI GCTCTTC 1 cut(s) 139
PfeI GAWTC 3 cut(s) 60, 297, 485
Ppu21I YACGTR 1 cut(s) 163
PscI ACATGT 1 cut(s) 170
Psp6I CCWGG 2 cut(s) 134, 372
PspGI CCWGG 2 cut(s) 134, 372
PspPI GGNCC 2 cut(s) 330, 456
PsuI RGATCY 1 cut(s) 310
RsaI GTAC 1 cut(s) 551
RsaNI GTAC 1 cut(s) 550
RseI CAYNNNNRTG 2 cut(s) 107, 420
SapI GCTCTTC 1 cut(s) 139
SaqAI TTAA 6 cut(s) 225, 303, 384, 444, 470, 531
Sau3AI GATC 4 cut(s) 127, 310, 402, 463
Sau96I GGNCC 2 cut(s) 330, 456
ScrFI CCNGG 4 cut(s) 136, 366, 374, 400
SduI GDGCHC 2 cut(s) 38, 116
SetI ASST 7 cut(s) 68, 165, 216, 236, 268, 503, 548
SfaNI GCATC 2 cut(s) 110, 359
SfcI CTRYAG 1 cut(s) 358
SinI GGWCC 2 cut(s) 330, 456
SmiMI CAYNNNNRTG 2 cut(s) 107, 420
Sse9I AATT 1 cut(s) 532
SsiI CCGC 1 cut(s) 276
SspMI CTAG 1 cut(s) 30
StyD4I CCNGG 4 cut(s) 134, 364, 372, 398
TaaI ACNGT 2 cut(s) 40, 511
TaiI ACGT 1 cut(s) 165
TaqI TCGA 1 cut(s) 178
TasI AATT 1 cut(s) 532
TatI WGTACW 1 cut(s) 549
TfiI GAWTC 3 cut(s) 60, 297, 485
Tru1I TTAA 6 cut(s) 225, 303, 384, 444, 470, 531
Tru9I TTAA 6 cut(s) 225, 303, 384, 444, 470, 531
TscAI CASTG 1 cut(s) 482
TseFI GTSAC 2 cut(s) 83, 163
Tsp45I GTSAC 2 cut(s) 83, 163
TspDTI ATGAA 2 cut(s) 4, 289
TspGWI ACGGA 1 cut(s) 456
TspRI CASTG 1 cut(s) 482
VpaK11BI GGWCC 2 cut(s) 330, 456
XceI RCATGY 1 cut(s) 174
XmiI GTMKAC 2 cut(s) 354, 503
XspI CTAG 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.