RLG00000023454
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013425
Physical Location & Seq
Reverse (-)
24014467 .. 24018445
3979 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023454

Sequence Viewer

Length: 2277 bp
ATGGCCACACTCTTCTTCTTTCCCTTTCTACTTACCCTCCTCCACTCCACCTCCGCCACGTCATCATCCCCACCCGCCATCGAGCTCTTCCGCAACCTCGCCGCCGCTCTCTCCGTCACCGACTTCGGGGCCACCGGAGACGGCGTCACCTACGACACCTCCGCCATCCAATCAGCCATCGACAGCTGCCGCACCACGCCCTGCCACGTCACCTTCCCGAGTGGAGGCACCTACCTGACCGCGACGATCCGCCTCAAATCCGGCGTCGTTTTGCACATCGAAGAAGGCGCGACTCTCCTCGGCGGCACGCGGCTGCGGGACTACCCGACCGAGCAGGAGCGGTGGTACGTGGTGCTGGCCGAGAACGCCACCGATGTGGGGATCACCGGCGGCGGAGTCATCGACGGCCAGGGGCTTGAGTTCGTGAAGAGATTCGATGAGAAGAAGAATGTGATGGTCAGCTGGAACTGGACCGGGGCTTGTTTGGGAGACGAGTGCCGGCCCAGGCTCGTCGGCTTCATTAACTGTAGAAATGTCAAGCTTTCCAATATTGAGTTGCGTCAGCCGGCTTACTGGTGCTTGCACATTGTACGTTGTGAAAGTATTACTATTCAAGATGTTTCAATTTATGGAGATTTCAATACACCCAATAATGATGGGATAGATATTGAGGATTCAAATAATACGGTCATTACTAGATGCCATATCGATACCGGAGATGATGCTATCTGTCCAAAGACGTACACTGGCCCCCTTTATAACTTAACTGTAACAAATTGCTGGATTCGGACCAAGTCTTCAGCAATCAAACTTGGCAGTGCTAGTTGGTTTGATTTTAAAGGTCTGGTGTTTGACAATATCACTATTGTAGATTCTCATAGAGGGCTGGGATTCCAGATACGTGATGGAGGAAATGTGAGTGATGTTATCTTCTCAAACATTAATATCAGCACAAGATACTATCATCAATCCTGGTGGGGAAGAGCAGAACCTATTTATGTGACTACTTGTCCACGCGACTCATATTCAAAGGAGGGTTCAATCTCTAACCTACTCTTTGTGAACATCAGTGCAAATTCAGAGAATGGTATATTTTTATCAGGCTCAAAAAAGGGGTTTCTTAGTGATTTGAGATTCATCAACTTGAATCTGACCTACAGCAGACGGACCAACAATGCGGGTGGGTTGGTAGACTACAGACCAGGATGCCAAGGGCTGGTAAAGCACAGCACAGCTGGTTTCATCATGGAGCACATTAATGGCTTAGTGTTTGACAATATTTACATGAGATGGTCCGATGAGCAATGGTCTGCTGAGCAATTAGGACAGTGGAATAATCCCTTGGATTTCAGATGGACCAACTCAATCTTTTCGCTTAATCAGAAGAGCTTACAGAAGAAAACATCGATCGCCGCTTTGCAGATAACTCTATCAATGCCTTCCATGGTCTCGACCTGCTTCTCACCTCCATCAACGCCTCATCCTCGCCTCACTCTCAAAGGCTCACTCATTCCTAACCTTAGACCTGCCATAACCCTTCCTCACCATCTTTTCCAAACACCGGCTTTCCCACTCTCAGGATGCAGCTTTTCTGCAAAGGTTCTAGAATCTAGGGTTCTAATGCCCATTACAAGAGCAAGTTTTGTTGCTGTCACTGCCATTTGGCCCTGCCGAGGCGGTGCTGATCTGAAGAACAATAAGTTACCTAACTTGAGAACAAAGGGTTTAGGGTTTAGGGTTGATGGGAGATCATCAAGGCAACGAGCAGTACGACCCCAGAATGATGGTGAGCTACTTTCGTCTGAGGAGTTGTACAAGACCATGAACATCCTCGTCTCTATTTTCTCGTTCCTATCTGTAGATGGTGATCCAAGTAAAGGAGTTGAAGAAGAAAGGTGGAAGTTGATTTGGCAGTACATAACCTCTAATGGCGGTGCTGTTATTCCAGTTGAAGAGTATGCTCCGCATCTGGTCACATTGCTTAAGCAAAAGATTGAAATCGAAAAGAGAAATCGAGCAATGCAGCAGTCAACTCGATTGGCGCTTGAATTTTCAGACGAAAGCCAGCCAAGACGAAAGCTCCTCAGTTTTATTAGTCTCTGTTGGTGCTGCTTTCTACGGGTAGCTGCTTTTCTTGCCAAGGGTGTCTCCTTTCTCTTGCTCTCTTTTGGTGGCTCCTTTGTATTGCTGTGTTATTTTACTTATGTATTGGAAAACAACCTAAAGACTTGTAAAGTGTTCGGTTTGTTTTTGTCATACTGGGGAAAATTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

759

Amino Acids

84.5

Weight (kDa)

8.62

Isoelectric Point (pI)

46.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 38 - 91 2.2e-10 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 68 - 389 5e-23 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013425)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57790 AT3G57790
fragaria_vesca FvH4_6g22970
malus_domestica MD03G1162500.v1.1 MD12G1064100.v1.1
prunus_persica Prupe.7G070000_v2.0.a1
pyrus_communis pycom12g05590
rosa_chinensis RchiOBHm_Chr3g0480761
rosa_laevigata RLG00000023454
rosa_multiflora Rmu_co8289587.1_g000001 Rmu_sc0003902.1_g000012
rosa_roxburghii Rroxscaffold_6G00401090
rosa_rugosa Rorug03G0185600
rosa_samantha Rh3AG237700 Rh3BG271100 Rh3CG267200 Rh3DG263200
rosa_wichuraiana Rw3G021290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 759
Acc36I ACCTGC 2 cut(s) 1463, 1534
AccB1I GGYRCC 1 cut(s) 227
AccB7I CCANNNNNTGG 1 cut(s) 1216
AccBSI CCGCTC 2 cut(s) 107, 340
AccI GTMKAC 1 cut(s) 1191
AccII CGCG 4 cut(s) 242, 290, 310, 1017
AclWI GGATC 3 cut(s) 241, 389, 1862
AcoI YGGCCR 3 cut(s) 3, 357, 406
AcsI RAATTY 2 cut(s) 1075, 2048
AcuI CTGAAG 2 cut(s) 783, 1709
AcyI GRCGYC 2 cut(s) 144, 264
AfaI GTAC 6 cut(s) 347, 591, 743, 1770, 1814, 1916
AfiI CCNNNNNNNGG 8 cut(s) 126, 224, 378, 1216, 1561, 1577, 1673, 1877
AflII CTTAAG 1 cut(s) 1982
AhdI GACNNNNNGTC 1 cut(s) 1008
AjiI CACGTC 2 cut(s) 60, 208
AjnI CCWGG 4 cut(s) 408, 503, 971, 1201
AjuI GAANNNNNNNTTGG 2 cut(s) 1325, 1357
AleI CACNNNNGTG 1 cut(s) 374
Alw21I GWGCWC 2 cut(s) 87, 1254
Alw26I GTCTC 6 cut(s) 132, 483, 1453, 1840, 2102, 2152
AlwI GGATC 3 cut(s) 241, 389, 1862
Ama87I CYCGRG 1 cut(s) 217
AoxI GGCC 7 cut(s) 3, 129, 357, 406, 500, 748, 1664
ApeKI GCWGC 6 cut(s) 186, 313, 1584, 2023, 2109, 2126
ApoI RAATTY 2 cut(s) 1075, 2048
ArsI GACNNNNNNTTYG 2 cut(s) 249, 281
AseI ATTAAT 2 cut(s) 942, 1257
Asp700I GAANNNNTTC 1 cut(s) 431
AspLEI GCGC 2 cut(s) 290, 2044
AspS9I GGNCC 9 cut(s) 129, 471, 501, 749, 789, 1167, 1293, 1356, 1665
AsuC2I CCSGG 1 cut(s) 475
AsuHPI GGTGA 8 cut(s) 109, 139, 202, 376, 1455, 1535, 1799, 1877
AvaI CYCGRG 1 cut(s) 217
AvaII GGWCC 5 cut(s) 471, 789, 1167, 1293, 1356
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 227
BanII GRGCYC 1 cut(s) 87
BbsI GAAGAC 1 cut(s) 789
Bbv12I GWGCWC 2 cut(s) 87, 1254
BbvI GCAGC 6 cut(s) 173, 300, 1596, 2035, 2096, 2113
BceAI ACGGC 2 cut(s) 157, 421
BcgI CGANNNNNNTGC 2 cut(s) 2015, 2049
BciT130I CCWGG 4 cut(s) 410, 505, 973, 1203
BcnI CCSGG 1 cut(s) 475
BcoDI GTCTC 6 cut(s) 132, 483, 1453, 1840, 2102, 2152
BfaI CTAG 4 cut(s) 696, 822, 1604, 1611
BfmI CTRYAG 4 cut(s) 526, 1156, 1195, 1857
BfoI RGCGCY 1 cut(s) 2045
BfrI CTTAAG 1 cut(s) 1982
BfuAI ACCTGC 2 cut(s) 1463, 1534
BlpI GCTNAGC 1 cut(s) 1314
Bme1390I CCNGG 5 cut(s) 410, 475, 505, 973, 1203
Bme18I GGWCC 5 cut(s) 471, 789, 1167, 1293, 1356
BmeRI GACNNNNNGTC 1 cut(s) 1008
BmeT110I CYCGRG 1 cut(s) 217
BmgBI CACGTC 2 cut(s) 60, 208
BmgT120I GGNCC 9 cut(s) 129, 471, 501, 749, 789, 1167, 1293, 1356, 1665
BmiI GGNNCC 4 cut(s) 130, 229, 751, 2176
BmrFI CCNGG 5 cut(s) 410, 475, 505, 973, 1203
BmrI ACTGGG 1 cut(s) 2269
BmsI GCATC 5 cut(s) 689, 712, 1196, 1571, 1975
BmuI ACTGGG 1 cut(s) 2269
BpiI GAAGAC 1 cut(s) 789
Bpu1102I GCTNAGC 1 cut(s) 1314
BpuEI CTTGAG 2 cut(s) 437, 1732
BpuMI CCSGG 1 cut(s) 475
Bsa29I ATCGAT 2 cut(s) 708, 1406
BsaAI YACGTR 2 cut(s) 349, 902
BsaBI GATNNNNATC 2 cut(s) 1866, 1997
BsaHI GRCGYC 2 cut(s) 144, 264
BsaI GGTCTC 1 cut(s) 1453
BsaJI CCNNGG 9 cut(s) 298, 409, 474, 503, 1210, 1341, 1443, 1672, 2139
BsaWI WCCGGW 2 cut(s) 134, 713
Bsc4I CCNNNNNNNGG 8 cut(s) 126, 224, 378, 1216, 1561, 1577, 1673, 1877
Bse118I RCCGGY 4 cut(s) 386, 498, 565, 1561
Bse1I ACTGG 5 cut(s) 473, 578, 751, 1946, 2264
Bse3DI GCAATG 3 cut(s) 1310, 1976, 2025
Bse8I GATNNNNATC 2 cut(s) 1866, 1997
BseBI CCWGG 4 cut(s) 410, 505, 973, 1203
BseCI ATCGAT 2 cut(s) 708, 1406
BseDI CCNNGG 9 cut(s) 298, 409, 474, 503, 1210, 1341, 1443, 1672, 2139
BseGI GGATG 6 cut(s) 65, 165, 1211, 1480, 1586, 1827
BseJI GATNNNNATC 2 cut(s) 1866, 1997
BseLI CCNNNNNNNGG 8 cut(s) 126, 224, 378, 1216, 1561, 1577, 1673, 1877
BseMI GCAATG 3 cut(s) 1310, 1976, 2025
BseMII CTCAG 4 cut(s) 1305, 1590, 1794, 2098
BseNI ACTGG 5 cut(s) 473, 578, 751, 1946, 2264
BseRI GAGGAG 4 cut(s) 29, 287, 1820, 2072
BseXI GCAGC 6 cut(s) 173, 300, 1596, 2035, 2096, 2113
BseYI CCCAGC 1 cut(s) 886
Bsh1236I CGCG 4 cut(s) 242, 290, 310, 1017
Bsh1285I CGRYCG 2 cut(s) 330, 1410
BshFI GGCC 7 cut(s) 5, 131, 359, 408, 502, 750, 1666
BshNI GGYRCC 1 cut(s) 227
BshVI ATCGAT 2 cut(s) 708, 1406
BsiEI CGRYCG 2 cut(s) 330, 1410
BsiHKAI GWGCWC 2 cut(s) 87, 1254
BsiHKCI CYCGRG 1 cut(s) 217
BsiSI CCGG 8 cut(s) 135, 261, 387, 474, 499, 566, 714, 1562
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 8 cut(s) 126, 224, 378, 1216, 1561, 1577, 1673, 1877
BsmAI GTCTC 6 cut(s) 132, 483, 1453, 1840, 2102, 2152
BsmBI CGTCTC 3 cut(s) 132, 483, 1840
BsmFI GGGAC 1 cut(s) 332
BsnI GGCC 7 cut(s) 5, 131, 359, 408, 502, 750, 1666
Bso31I GGTCTC 1 cut(s) 1453
BsoBI CYCGRG 1 cut(s) 217
Bsp1286I GDGCHC 2 cut(s) 87, 1254
Bsp1407I TGTACA 1 cut(s) 1812
Bsp143I GATC 6 cut(s) 246, 381, 1407, 1684, 1748, 1867
Bsp1720I GCTNAGC 1 cut(s) 1314
Bsp19I CCATGG 1 cut(s) 1443
BspANI GGCC 7 cut(s) 5, 131, 359, 408, 502, 750, 1666
BspCNI CTCAG 4 cut(s) 1306, 1589, 1795, 2097
BspDI ATCGAT 2 cut(s) 708, 1406
BspFNI CGCG 4 cut(s) 242, 290, 310, 1017
BspLI GGNNCC 4 cut(s) 130, 229, 751, 2176
BspMI ACCTGC 2 cut(s) 1463, 1534
BspPI GGATC 3 cut(s) 241, 389, 1862
BspQI GCTCTTC 3 cut(s) 92, 976, 1379
BspT107I GGYRCC 1 cut(s) 227
BspTI CTTAAG 1 cut(s) 1982
BspTNI GGTCTC 1 cut(s) 1453
BsrBI CCGCTC 2 cut(s) 107, 340
BsrDI GCAATG 3 cut(s) 1310, 1976, 2025
BsrFI RCCGGY 4 cut(s) 386, 498, 565, 1561
BsrGI TGTACA 1 cut(s) 1812
BsrI ACTGG 5 cut(s) 473, 578, 751, 1946, 2264
BssAI RCCGGY 4 cut(s) 386, 498, 565, 1561
BssECI CCNNGG 9 cut(s) 298, 409, 474, 503, 1210, 1341, 1443, 1672, 2139
BssMI GATC 6 cut(s) 246, 381, 1407, 1684, 1748, 1867
BssNI GRCGYC 2 cut(s) 144, 264
BssT1I CCWWGG 4 cut(s) 1210, 1341, 1443, 2139
Bst2UI CCWGG 4 cut(s) 410, 505, 973, 1203
Bst4CI ACNGT 4 cut(s) 527, 688, 769, 1329
Bst6I CTCTTC 6 cut(s) 17, 92, 422, 976, 1379, 1947
BstACI GRCGYC 2 cut(s) 144, 264
BstAFI CTTAAG 1 cut(s) 1982
BstAUI TGTACA 1 cut(s) 1812
BstBAI YACGTR 2 cut(s) 349, 902
BstC8I GCNNGC 6 cut(s) 308, 357, 500, 567, 581, 2066
BstDEI CTNAG 7 cut(s) 1121, 1264, 1314, 1520, 1576, 1803, 2084
BstDSI CCRYGG 1 cut(s) 1443
BstF5I GGATG 6 cut(s) 65, 165, 1211, 1480, 1586, 1827
BstFNI CGCG 4 cut(s) 242, 290, 310, 1017
BstH2I RGCGCY 1 cut(s) 2045
BstHHI GCGC 2 cut(s) 290, 2044
BstKTI GATC 6 cut(s) 249, 384, 1410, 1687, 1751, 1870
BstMAI GTCTC 6 cut(s) 132, 483, 1453, 1840, 2102, 2152
BstMBI GATC 6 cut(s) 246, 381, 1407, 1684, 1748, 1867
BstMCI CGRYCG 2 cut(s) 330, 1410
BstMWI GCNNNNNNNGC 4 cut(s) 365, 1222, 1655, 2135
BstNI CCWGG 4 cut(s) 410, 505, 973, 1203
BstSCI CCNGG 5 cut(s) 408, 473, 503, 971, 1201
BstSFI CTRYAG 4 cut(s) 526, 1156, 1195, 1857
BstUI CGCG 4 cut(s) 242, 290, 310, 1017
BstV1I GCAGC 6 cut(s) 173, 300, 1596, 2035, 2096, 2113
BstV2I GAAGAC 1 cut(s) 789
BstXI CCANNNNNNTGG 2 cut(s) 376, 1784
Bsu15I ATCGAT 2 cut(s) 708, 1406
BsuRI GGCC 7 cut(s) 5, 131, 359, 408, 502, 750, 1666
BsuTUI ATCGAT 2 cut(s) 708, 1406
BtgI CCRYGG 1 cut(s) 1443
BtrI CACGTC 2 cut(s) 60, 208
BtsCI GGATG 6 cut(s) 65, 165, 1211, 1480, 1586, 1827
BtsI GCAGTG 2 cut(s) 823, 1653
BtsIMutI CAGTG 5 cut(s) 744, 823, 1075, 1334, 1653
BveI ACCTGC 2 cut(s) 1463, 1534
Cac8I GCNNGC 6 cut(s) 308, 357, 500, 567, 581, 2066
CfoI GCGC 2 cut(s) 290, 2044
Cfr10I RCCGGY 4 cut(s) 386, 498, 565, 1561
Cfr13I GGNCC 9 cut(s) 129, 471, 501, 749, 789, 1167, 1293, 1356, 1665
ClaI ATCGAT 2 cut(s) 708, 1406
CseI GACGC 3 cut(s) 133, 253, 548
Csp6I GTAC 6 cut(s) 346, 590, 742, 1769, 1813, 1915
CspCI CAANNNNNGTGG 4 cut(s) 956, 991, 1162, 1197
CviAII CATG 4 cut(s) 1246, 1285, 1444, 1822
CviQI GTAC 6 cut(s) 346, 590, 742, 1769, 1813, 1915
DdeI CTNAG 7 cut(s) 1121, 1264, 1314, 1520, 1576, 1803, 2084
DpnI GATC 6 cut(s) 248, 383, 1409, 1686, 1750, 1869
DpnII GATC 6 cut(s) 246, 381, 1407, 1684, 1748, 1867
DraI TTTAAA 1 cut(s) 838
DriI GACNNNNNGTC 1 cut(s) 1008
EaeI YGGCCR 3 cut(s) 3, 357, 406
Eam1104I CTCTTC 6 cut(s) 17, 92, 422, 976, 1379, 1947
Eam1105I GACNNNNNGTC 1 cut(s) 1008
EarI CTCTTC 6 cut(s) 17, 92, 422, 976, 1379, 1947
EciI GGCGGA 4 cut(s) 43, 151, 239, 408
Ecl136II GAGCTC 1 cut(s) 85
Eco130I CCWWGG 4 cut(s) 1210, 1341, 1443, 2139
Eco24I GRGCYC 1 cut(s) 87
Eco31I GGTCTC 1 cut(s) 1453
Eco47I GGWCC 5 cut(s) 471, 789, 1167, 1293, 1356
Eco53kI GAGCTC 1 cut(s) 85
Eco57I CTGAAG 2 cut(s) 783, 1709
Eco88I CYCGRG 1 cut(s) 217
EcoICRI GAGCTC 1 cut(s) 85
EcoRII CCWGG 4 cut(s) 408, 503, 971, 1201
EcoT14I CCWWGG 4 cut(s) 1210, 1341, 1443, 2139
EcoT38I GRGCYC 1 cut(s) 87
ErhI CCWWGG 4 cut(s) 1210, 1341, 1443, 2139
Esp3I CGTCTC 3 cut(s) 132, 483, 1840
FaeI CATG 4 cut(s) 1249, 1288, 1447, 1825
FaqI GGGAC 1 cut(s) 332
FatI CATG 4 cut(s) 1245, 1284, 1443, 1821
FauI CCCGC 3 cut(s) 82, 309, 1171
FblI GTMKAC 1 cut(s) 1191
FokI GGATG 6 cut(s) 52, 152, 1218, 1467, 1593, 1814
FriOI GRGCYC 1 cut(s) 87
FspBI CTAG 4 cut(s) 696, 822, 1604, 1611
GlaI GCGC 2 cut(s) 289, 2043
GsaI CCCAGC 1 cut(s) 890
HaeII RGCGCY 1 cut(s) 2045
HaeIII GGCC 7 cut(s) 5, 131, 359, 408, 502, 750, 1666
HapII CCGG 8 cut(s) 135, 261, 387, 474, 499, 566, 714, 1562
HgaI GACGC 3 cut(s) 133, 253, 548
HhaI GCGC 2 cut(s) 290, 2044
Hin1I GRCGYC 2 cut(s) 144, 264
Hin1II CATG 4 cut(s) 1249, 1288, 1447, 1825
Hin6I GCGC 2 cut(s) 288, 2042
HinP1I GCGC 2 cut(s) 288, 2042
HincII GTYRAC 1 cut(s) 2031
HindII GTYRAC 1 cut(s) 2031
HindIII AAGCTT 1 cut(s) 539
HpaII CCGG 8 cut(s) 135, 261, 387, 474, 499, 566, 714, 1562
HphI GGTGA 8 cut(s) 109, 139, 202, 376, 1455, 1535, 1799, 1877
Hpy166II GTNNAC 5 cut(s) 744, 1013, 1063, 1192, 2031
Hpy188I TCNGA 9 cut(s) 789, 1081, 1152, 1297, 1352, 1383, 1689, 1804, 2056
Hpy188III TCNNGA 7 cut(s) 217, 424, 614, 895, 1450, 1578, 1604
Hpy8I GTNNAC 5 cut(s) 744, 1013, 1063, 1192, 2031
Hpy99I CGWCG 4 cut(s) 247, 269, 407, 515
HpyAV CCTTC 4 cut(s) 223, 278, 1449, 1547
HpyCH4III ACNGT 4 cut(s) 527, 688, 769, 1329
HpyCH4IV ACGT 6 cut(s) 59, 207, 348, 592, 740, 901
HpyCH4V TGCA 7 cut(s) 274, 583, 1073, 1420, 1584, 1595, 2023
HpyF10VI GCNNNNNNNGC 4 cut(s) 365, 1222, 1655, 2135
HpyF3I CTNAG 7 cut(s) 1121, 1264, 1314, 1520, 1576, 1803, 2084
HpySE526I ACGT 6 cut(s) 59, 207, 348, 592, 740, 901
Hsp92I GRCGYC 2 cut(s) 144, 264
Hsp92II CATG 4 cut(s) 1249, 1288, 1447, 1825
HspAI GCGC 2 cut(s) 288, 2042
KroI GCCGGC 2 cut(s) 498, 565
KroNI GCCGGC 2 cut(s) 500, 567
Kzo9I GATC 6 cut(s) 246, 381, 1407, 1684, 1748, 1867
LguI GCTCTTC 3 cut(s) 92, 976, 1379
LmnI GCTCC 5 cut(s) 337, 1249, 1966, 2085, 2180
Lsp1109I GCAGC 6 cut(s) 173, 300, 1596, 2035, 2096, 2113
LweI GCATC 5 cut(s) 689, 712, 1196, 1571, 1975
MaeI CTAG 4 cut(s) 696, 822, 1604, 1611
MaeII ACGT 6 cut(s) 59, 207, 348, 592, 740, 901
MaeIII GTNAC 8 cut(s) 115, 145, 208, 769, 1000, 1651, 1701, 1972
MalI GATC 6 cut(s) 248, 383, 1409, 1686, 1750, 1869
MbiI CCGCTC 2 cut(s) 107, 340
MboI GATC 6 cut(s) 246, 381, 1407, 1684, 1748, 1867
MhlI GDGCHC 2 cut(s) 87, 1254
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 624, 775, 1075, 1319, 2048, 2267
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 286, 405, 1013
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 2 cut(s) 498, 565
MroXI GAANNNNTTC 1 cut(s) 431
MscI TGGCCA 1 cut(s) 5
MseI TTAA 7 cut(s) 522, 764, 837, 942, 1257, 1377, 1983
MslI CAYNNNNRTG 2 cut(s) 374, 1257
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 186, 462, 1235
MspCI CTTAAG 1 cut(s) 1982
MspI CCGG 8 cut(s) 135, 261, 387, 474, 499, 566, 714, 1562
MspR9I CCNGG 5 cut(s) 410, 475, 505, 973, 1203
MvaI CCWGG 4 cut(s) 410, 505, 973, 1203
MvnI CGCG 4 cut(s) 242, 290, 310, 1017
MwoI GCNNNNNNNGC 4 cut(s) 365, 1222, 1655, 2135
NaeI GCCGGC 2 cut(s) 500, 567
NciI CCSGG 1 cut(s) 475
NcoI CCATGG 1 cut(s) 1443
NdeII GATC 6 cut(s) 246, 381, 1407, 1684, 1748, 1867
NgoMIV GCCGGC 2 cut(s) 498, 565
NlaIII CATG 4 cut(s) 1249, 1288, 1447, 1825
NlaIV GGNNCC 4 cut(s) 130, 229, 751, 2176
NmeAIII GCCGAG 3 cut(s) 279, 385, 1697
NmuCI GTSAC 6 cut(s) 115, 145, 208, 1000, 1651, 1972
OliI CACNNNNGTG 1 cut(s) 374
PciSI GCTCTTC 3 cut(s) 92, 976, 1379
PcsI WCGNNNNNNNCGW 1 cut(s) 1768
PdiI GCCGGC 2 cut(s) 500, 567
PdmI GAANNNNTTC 1 cut(s) 431
PfeI GAWTC 8 cut(s) 432, 674, 784, 872, 891, 1134, 1147, 1607
PflFI GACNNNGTC 2 cut(s) 143, 793
PflMI CCANNNNNTGG 1 cut(s) 1216
Ple19I CGATCG 1 cut(s) 1410
PleI GAGTC 3 cut(s) 286, 404, 1013
PpsI GAGTC 3 cut(s) 286, 404, 1013
Ppu21I YACGTR 2 cut(s) 349, 902
PshBI ATTAAT 2 cut(s) 942, 1257
PsiI TTATAA 1 cut(s) 759
Psp124BI GAGCTC 1 cut(s) 87
Psp6I CCWGG 4 cut(s) 408, 503, 971, 1201
PspFI CCCAGC 1 cut(s) 886
PspGI CCWGG 4 cut(s) 408, 503, 971, 1201
PspN4I GGNNCC 4 cut(s) 130, 229, 751, 2176
PspPI GGNCC 9 cut(s) 129, 471, 501, 749, 789, 1167, 1293, 1356, 1665
PsyI GACNNNGTC 2 cut(s) 143, 793
PvuI CGATCG 1 cut(s) 1410
PvuII CAGCTG 3 cut(s) 186, 462, 1235
RsaI GTAC 6 cut(s) 347, 591, 743, 1770, 1814, 1916
RsaNI GTAC 6 cut(s) 346, 590, 742, 1769, 1813, 1915
RseI CAYNNNNRTG 2 cut(s) 374, 1257
SacI GAGCTC 1 cut(s) 87
SapI GCTCTTC 3 cut(s) 92, 976, 1379
SaqAI TTAA 7 cut(s) 522, 764, 837, 942, 1257, 1377, 1983
Sau3AI GATC 6 cut(s) 246, 381, 1407, 1684, 1748, 1867
Sau96I GGNCC 9 cut(s) 129, 471, 501, 749, 789, 1167, 1293, 1356, 1665
SchI GAGTC 3 cut(s) 286, 405, 1013
ScrFI CCNGG 5 cut(s) 410, 475, 505, 973, 1203
SduI GDGCHC 2 cut(s) 87, 1254
SfaNI GCATC 5 cut(s) 689, 712, 1196, 1571, 1975
SfcI CTRYAG 4 cut(s) 526, 1156, 1195, 1857
SgrAI CRCCGGYG 1 cut(s) 386
SinI GGWCC 5 cut(s) 471, 789, 1167, 1293, 1356
SmiMI CAYNNNNRTG 2 cut(s) 374, 1257
SmlI CTYRAG 3 cut(s) 416, 1711, 1982
SmoI CTYRAG 3 cut(s) 416, 1711, 1982
Sse9I AATT 6 cut(s) 624, 775, 1075, 1319, 2048, 2267
SspI AATATT 2 cut(s) 550, 1279
SspMI CTAG 4 cut(s) 696, 822, 1604, 1611
SstI GAGCTC 1 cut(s) 87
StyD4I CCNGG 5 cut(s) 408, 473, 503, 971, 1201
StyI CCWWGG 4 cut(s) 1210, 1341, 1443, 2139
TaaI ACNGT 4 cut(s) 527, 688, 769, 1329
TaiI ACGT 6 cut(s) 62, 210, 351, 595, 743, 904
TaqII GACCGA 1 cut(s) 344
TasI AATT 6 cut(s) 624, 775, 1075, 1319, 2048, 2267
TatI WGTACW 2 cut(s) 1812, 1914
TauI GCSGC 7 cut(s) 104, 107, 192, 306, 313, 393, 1415
TfiI GAWTC 8 cut(s) 432, 674, 784, 872, 891, 1134, 1147, 1607
Tru1I TTAA 7 cut(s) 522, 764, 837, 942, 1257, 1377, 1983
Tru9I TTAA 7 cut(s) 522, 764, 837, 942, 1257, 1377, 1983
TscAI CASTG 5 cut(s) 751, 823, 1075, 1334, 1660
TseFI GTSAC 6 cut(s) 115, 145, 208, 1000, 1651, 1972
TseI GCWGC 6 cut(s) 186, 313, 1584, 2023, 2109, 2126
Tsp45I GTSAC 6 cut(s) 115, 145, 208, 1000, 1651, 1972
TspDTI ATGAA 4 cut(s) 508, 1126, 1231, 1838
TspGWI ACGGA 2 cut(s) 103, 1180
TspRI CASTG 5 cut(s) 751, 823, 1075, 1334, 1660
Tth111I GACNNNGTC 2 cut(s) 143, 793
Van91I CCANNNNNTGG 1 cut(s) 1216
Vha464I CTTAAG 1 cut(s) 1982
VpaK11BI GGWCC 5 cut(s) 471, 789, 1167, 1293, 1356
VspI ATTAAT 2 cut(s) 942, 1257
XapI RAATTY 2 cut(s) 1075, 2048
XbaI TCTAGA 1 cut(s) 1603
XcmI CCANNNNNNNNNTGG 1 cut(s) 902
XmiI GTMKAC 1 cut(s) 1191
XmnI GAANNNNTTC 1 cut(s) 431
XspI CTAG 4 cut(s) 696, 822, 1604, 1611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.