RchiOBHm_Chr3g0480761
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0013425
Physical Location & Seq
Forward (+)
26617012 .. 26619831
2820 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44577

Sequence Viewer

Length: 1518 bp
ATGTTTTTCTTTTCCTTCTATTTTAAGCCATCAAGCTTTTTAGTCAGGTACAGAGCCAAACTGAGTAACTGGTTGTTGGACTTGAAAACAGAGCCCCAATCCGAAATGGCAACACTCTTCTTCTTCCCCTTTCTACTTACTCTCCTCCACTCCACCTCCGCCACGTCATCATCCCCACCCACCATCGAGCTCTTCCGCAACCTCACCGCCGCTCTCTCCGTCACCGACTTCGGCGCCACCGGAGACGGCGTCACCTACGACACCTCCGCCATCGAATCAGCCATCGACAGCTGCCGCACCACCCCCTGCCACGTCACCTTCCCGAGTGGAGGCACCTACCTGACCGCGACGATCCGCCTCAAATCCGGCGTCGTTTTGCACATCAAAGAGGGCGCGACTCTCCTCGGCGGCACGCGGCTGCGGGACTACCCGATCGAGCAGGAGCGGTGGTACGTGGTGCTGGCCGAGAACACCACCGATGTAGGGATCACCGGCGGCGGAGTCGTCGACGGCCAGGGTCTTGAGTTCGTGAAGAGATTCGATGAGAAGAAGAATGTGATGGTCAGCTGGAACTGGACCGGGGCTTGTTTGGGAGACGAGTGCCGGCCCAGGCTCGTCGGCTTCATTGACTGCAGAAATGTCAAGCTTTCCAATATTGAGTTGCGTCAGCCGGCTTACTGGTGCTTGCACATTGTACGTTGTGAACACATTACTATTCAAGATGTTTCAATTTATGGAGATTTCAATACACCCAATAATGATGGGATAGATATTGAGGATTCAAATAATACAGTCATTACTAGATGCCATATCGATACCGGAGATGATGCTATCTGTCCAAAGACGTACACTGGCCCCCTTTATAACTTAACTGTAACAAATTGCTGGATTCGGACCAAATCTTCCGCAATCAAACTTGGCAGTGCTAGTTGGTTTGATTTTAAAGGTCTGGTGTTTGACAATATCACTATTGCAGATTCTCATAGAGGGCTGGGATTCCAGATACGTGATGGAGGAAATGTGAGTGATGTTATCTTTTCAAACATTAATATCAGCACAAGATACTATCATCAATCCTGGTGGGGAAGAGCAGAACCTATTTATGTGACTACTTGTCCACGCGATTCATATGCAAAGGAGGGTTCAATCTCTAACCTACTCTTTGTGAACATCAGTGCAAATTCAGAGAATGGTATATTTTTATCAGGCTCTAAAAAGGGGCTTCTTAGTGATTTGAGATTCATCAACTTGAATCTGACCTACAGCAGACGGACCAACAATGTGGGTGGGTTGGTAGACTACAGACCAGGATGCCAAGGGCTGGTAAAGCACAGCACAGCTGGATTCATCATGGAGTACATTAATGGCTTAGTGTTTGACAATATTTACATGAGATGGTCCGATGAGCAATGGTCTGCTGAGCAATTAGGACAGTGGAATAATCCCTTGGATTTCAGGTCTTCTACTGTAAATAACATATCTATGCTTAATTTTCATTCAAGCCTATACACACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

505

Amino Acids

56.64

Weight (kDa)

5.96

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 73 - 126 7.1e-10 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 104 - 426 3.4e-23 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013425)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57790 AT3G57790
fragaria_vesca FvH4_6g22970
malus_domestica MD03G1162500.v1.1 MD12G1064100.v1.1
prunus_persica Prupe.7G070000_v2.0.a1
pyrus_communis pycom12g05590
rosa_chinensis RchiOBHm_Chr3g0480761
rosa_laevigata RLG00000023454
rosa_multiflora Rmu_co8289587.1_g000001 Rmu_sc0003902.1_g000012
rosa_roxburghii Rroxscaffold_6G00401090
rosa_rugosa Rorug03G0185600
rosa_samantha Rh3AG237700 Rh3BG271100 Rh3CG267200 Rh3DG263200
rosa_wichuraiana Rw3G021290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 864
AccB1I GGYRCC 2 cut(s) 233, 332
AccB7I CCANNNNNTGG 1 cut(s) 1321
AccBSI CCGCTC 2 cut(s) 212, 445
AccI GTMKAC 2 cut(s) 507, 1296
AccII CGCG 4 cut(s) 347, 395, 415, 1122
AclWI GGATC 2 cut(s) 346, 494
AcoI YGGCCR 2 cut(s) 462, 511
AcsI RAATTY 1 cut(s) 1180
AcyI GRCGYC 3 cut(s) 234, 249, 369
AdeI CACNNNGTG 1 cut(s) 1515
AfaI GTAC 5 cut(s) 50, 452, 696, 848, 1358
AfiI CCNNNNNNNGG 3 cut(s) 329, 483, 1321
AgsI TTSAA 9 cut(s) 85, 719, 729, 745, 783, 1041, 1146, 1252, 1500
AhdI GACNNNNNGTC 1 cut(s) 1113
AjiI CACGTC 2 cut(s) 165, 313
AjnI CCWGG 4 cut(s) 513, 608, 1076, 1306
AjuI GAANNNNNNNTTGG 2 cut(s) 1430, 1462
AluBI AGCT 6 cut(s) 36, 190, 291, 567, 646, 1340
AluI AGCT 6 cut(s) 36, 190, 291, 567, 646, 1340
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 2 cut(s) 237, 588
AlwI GGATC 2 cut(s) 346, 494
Ama87I CYCGRG 1 cut(s) 322
AoxI GGCC 4 cut(s) 462, 511, 605, 853
ApeKI GCWGC 2 cut(s) 291, 418
ApoI RAATTY 1 cut(s) 1180
ArsI GACNNNNNNTTYG 2 cut(s) 354, 386
AseI ATTAAT 2 cut(s) 1047, 1362
Asp700I GAANNNNTTC 1 cut(s) 536
AspLEI GCGC 2 cut(s) 236, 395
AspS9I GGNCC 6 cut(s) 576, 606, 854, 894, 1272, 1398
AsuC2I CCSGG 1 cut(s) 580
AsuHPI GGTGA 5 cut(s) 196, 214, 244, 307, 481
AvaI CYCGRG 1 cut(s) 322
AvaII GGWCC 4 cut(s) 576, 894, 1272, 1398
BanI GGYRCC 2 cut(s) 233, 332
BanII GRGCYC 2 cut(s) 96, 192
BbsI GAAGAC 1 cut(s) 1452
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 278, 405
BccI CCATC 8 cut(s) 37, 191, 278, 290, 553, 755, 1004, 1389
BceAI ACGGC 2 cut(s) 262, 526
BcgI CGANNNNNNTGC 2 cut(s) 1112, 1146
BciT130I CCWGG 4 cut(s) 515, 610, 1078, 1308
BcnI CCSGG 1 cut(s) 580
BcoDI GTCTC 2 cut(s) 237, 588
BfaI CTAG 2 cut(s) 801, 927
BfmI CTRYAG 3 cut(s) 631, 1261, 1300
BfoI RGCGCY 1 cut(s) 237
BisI GCNGC 7 cut(s) 210, 292, 295, 409, 416, 419, 496
BlpI GCTNAGC 1 cut(s) 1419
BlsI GCNGC 7 cut(s) 211, 293, 296, 410, 417, 420, 497
Bme1390I CCNGG 5 cut(s) 515, 580, 610, 1078, 1308
Bme18I GGWCC 4 cut(s) 576, 894, 1272, 1398
BmeRI GACNNNNNGTC 1 cut(s) 1113
BmeT110I CYCGRG 1 cut(s) 322
BmgBI CACGTC 2 cut(s) 165, 313
BmgT120I GGNCC 6 cut(s) 576, 606, 854, 894, 1272, 1398
BmiI GGNNCC 3 cut(s) 235, 334, 856
BmrFI CCNGG 5 cut(s) 515, 580, 610, 1078, 1308
BmsI GCATC 3 cut(s) 794, 817, 1301
BpiI GAAGAC 1 cut(s) 1452
Bpu1102I GCTNAGC 1 cut(s) 1419
BpuEI CTTGAG 1 cut(s) 542
BpuMI CCSGG 1 cut(s) 580
Bsa29I ATCGAT 1 cut(s) 813
BsaAI YACGTR 2 cut(s) 454, 1007
BsaHI GRCGYC 3 cut(s) 234, 249, 369
BsaJI CCNNGG 6 cut(s) 403, 514, 579, 608, 1315, 1446
BsaWI WCCGGW 2 cut(s) 239, 818
Bsc4I CCNNNNNNNGG 3 cut(s) 329, 483, 1321
Bse118I RCCGGY 3 cut(s) 491, 603, 670
Bse1I ACTGG 4 cut(s) 74, 578, 683, 856
Bse3DI GCAATG 1 cut(s) 1415
BseBI CCWGG 4 cut(s) 515, 610, 1078, 1308
BseCI ATCGAT 1 cut(s) 813
BseDI CCNNGG 6 cut(s) 403, 514, 579, 608, 1315, 1446
BseGI GGATG 2 cut(s) 170, 1316
BseLI CCNNNNNNNGG 3 cut(s) 329, 483, 1321
BseMI GCAATG 1 cut(s) 1415
BseMII CTCAG 2 cut(s) 53, 1410
BseNI ACTGG 4 cut(s) 74, 578, 683, 856
BseRI GAGGAG 2 cut(s) 134, 392
BseXI GCAGC 2 cut(s) 278, 405
BseYI CCCAGC 1 cut(s) 991
Bsh1236I CGCG 4 cut(s) 347, 395, 415, 1122
Bsh1285I CGRYCG 1 cut(s) 435
BshFI GGCC 4 cut(s) 464, 513, 607, 855
BshNI GGYRCC 2 cut(s) 233, 332
BshVI ATCGAT 1 cut(s) 813
BsiEI CGRYCG 1 cut(s) 435
BsiHKAI GWGCWC 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 322
BsiSI CCGG 7 cut(s) 240, 366, 492, 579, 604, 671, 819
BslFI GGGAC 1 cut(s) 437
BslI CCNNNNNNNGG 3 cut(s) 329, 483, 1321
BsmAI GTCTC 2 cut(s) 237, 588
BsmBI CGTCTC 2 cut(s) 237, 588
BsmFI GGGAC 1 cut(s) 437
BsnI GGCC 4 cut(s) 464, 513, 607, 855
BsoBI CYCGRG 1 cut(s) 322
Bsp1286I GDGCHC 2 cut(s) 96, 192
Bsp143I GATC 3 cut(s) 351, 432, 486
Bsp1720I GCTNAGC 1 cut(s) 1419
BspANI GGCC 4 cut(s) 464, 513, 607, 855
BspCNI CTCAG 2 cut(s) 54, 1411
BspDI ATCGAT 1 cut(s) 813
BspFNI CGCG 4 cut(s) 347, 395, 415, 1122
BspLI GGNNCC 3 cut(s) 235, 334, 856
BspMAI CTGCAG 1 cut(s) 635
BspPI GGATC 2 cut(s) 346, 494
BspQI GCTCTTC 2 cut(s) 197, 1081
BspT107I GGYRCC 2 cut(s) 233, 332
BsrBI CCGCTC 2 cut(s) 212, 445
BsrDI GCAATG 1 cut(s) 1415
BsrFI RCCGGY 3 cut(s) 491, 603, 670
BsrI ACTGG 4 cut(s) 74, 578, 683, 856
BssAI RCCGGY 3 cut(s) 491, 603, 670
BssECI CCNNGG 6 cut(s) 403, 514, 579, 608, 1315, 1446
BssMI GATC 3 cut(s) 351, 432, 486
BssNI GRCGYC 3 cut(s) 234, 249, 369
BssT1I CCWWGG 2 cut(s) 1315, 1446
Bst2UI CCWGG 4 cut(s) 515, 610, 1078, 1308
Bst4CI ACNGT 5 cut(s) 793, 874, 1434, 1468, 1515
Bst6I CTCTTC 4 cut(s) 122, 197, 527, 1081
BstACI GRCGYC 3 cut(s) 234, 249, 369
BstBAI YACGTR 2 cut(s) 454, 1007
BstC8I GCNNGC 5 cut(s) 413, 462, 605, 672, 686
BstDEI CTNAG 4 cut(s) 62, 1226, 1369, 1419
BstF5I GGATG 2 cut(s) 170, 1316
BstFNI CGCG 4 cut(s) 347, 395, 415, 1122
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 2 cut(s) 236, 395
BstKTI GATC 3 cut(s) 354, 435, 489
BstMAI GTCTC 2 cut(s) 237, 588
BstMBI GATC 3 cut(s) 351, 432, 486
BstMCI CGRYCG 1 cut(s) 435
BstMWI GCNNNNNNNGC 1 cut(s) 1327
BstNI CCWGG 4 cut(s) 515, 610, 1078, 1308
BstSCI CCNGG 5 cut(s) 513, 578, 608, 1076, 1306
BstSFI CTRYAG 3 cut(s) 631, 1261, 1300
BstUI CGCG 4 cut(s) 347, 395, 415, 1122
BstV1I GCAGC 2 cut(s) 278, 405
BstV2I GAAGAC 1 cut(s) 1452
BstXI CCANNNNNNTGG 1 cut(s) 1282
Bsu15I ATCGAT 1 cut(s) 813
BsuRI GGCC 4 cut(s) 464, 513, 607, 855
BsuTUI ATCGAT 1 cut(s) 813
BtrI CACGTC 2 cut(s) 165, 313
BtsCI GGATG 2 cut(s) 170, 1316
BtsI GCAGTG 1 cut(s) 928
BtsIMutI CAGTG 4 cut(s) 849, 928, 1180, 1439
Cac8I GCNNGC 5 cut(s) 413, 462, 605, 672, 686
CfoI GCGC 2 cut(s) 236, 395
Cfr10I RCCGGY 3 cut(s) 491, 603, 670
Cfr13I GGNCC 6 cut(s) 576, 606, 854, 894, 1272, 1398
ClaI ATCGAT 1 cut(s) 813
CseI GACGC 3 cut(s) 238, 358, 653
Csp6I GTAC 5 cut(s) 49, 451, 695, 847, 1357
CspCI CAANNNNNGTGG 4 cut(s) 1061, 1096, 1267, 1302
CviAII CATG 2 cut(s) 1351, 1390
CviQI GTAC 5 cut(s) 49, 451, 695, 847, 1357
DdeI CTNAG 4 cut(s) 62, 1226, 1369, 1419
DinI GGCGCC 1 cut(s) 235
DpnI GATC 3 cut(s) 353, 434, 488
DpnII GATC 3 cut(s) 351, 432, 486
DraI TTTAAA 1 cut(s) 943
DraIII CACNNNGTG 1 cut(s) 1515
DriI GACNNNNNGTC 1 cut(s) 1113
EaeI YGGCCR 2 cut(s) 462, 511
Eam1104I CTCTTC 4 cut(s) 122, 197, 527, 1081
Eam1105I GACNNNNNGTC 1 cut(s) 1113
EarI CTCTTC 4 cut(s) 122, 197, 527, 1081
EciI GGCGGA 4 cut(s) 148, 256, 344, 513
Ecl136II GAGCTC 1 cut(s) 190
Eco130I CCWWGG 2 cut(s) 1315, 1446
Eco24I GRGCYC 2 cut(s) 96, 192
Eco47I GGWCC 4 cut(s) 576, 894, 1272, 1398
Eco53kI GAGCTC 1 cut(s) 190
Eco88I CYCGRG 1 cut(s) 322
EcoICRI GAGCTC 1 cut(s) 190
EcoRII CCWGG 4 cut(s) 513, 608, 1076, 1306
EcoT14I CCWWGG 2 cut(s) 1315, 1446
EcoT38I GRGCYC 2 cut(s) 96, 192
EgeI GGCGCC 1 cut(s) 235
EheI GGCGCC 1 cut(s) 235
ErhI CCWWGG 2 cut(s) 1315, 1446
Esp3I CGTCTC 2 cut(s) 237, 588
FaeI CATG 2 cut(s) 1354, 1393
FaqI GGGAC 1 cut(s) 437
FatI CATG 2 cut(s) 1350, 1389
FauI CCCGC 1 cut(s) 414
FauNDI CATATG 1 cut(s) 1129
FblI GTMKAC 2 cut(s) 507, 1296
Fnu4HI GCNGC 7 cut(s) 210, 292, 295, 409, 416, 419, 496
FokI GGATG 2 cut(s) 157, 1323
FriOI GRGCYC 2 cut(s) 96, 192
Fsp4HI GCNGC 7 cut(s) 210, 292, 295, 409, 416, 419, 496
FspBI CTAG 2 cut(s) 801, 927
GlaI GCGC 2 cut(s) 235, 394
GluI GCNGC 7 cut(s) 210, 292, 295, 409, 416, 419, 496
GsaI CCCAGC 1 cut(s) 995
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 4 cut(s) 464, 513, 607, 855
HapII CCGG 7 cut(s) 240, 366, 492, 579, 604, 671, 819
HgaI GACGC 3 cut(s) 238, 358, 653
HhaI GCGC 2 cut(s) 236, 395
Hin1I GRCGYC 3 cut(s) 234, 249, 369
Hin1II CATG 2 cut(s) 1354, 1393
Hin6I GCGC 2 cut(s) 234, 393
HinP1I GCGC 2 cut(s) 234, 393
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HindIII AAGCTT 2 cut(s) 34, 644
HpaII CCGG 7 cut(s) 240, 366, 492, 579, 604, 671, 819
HphI GGTGA 5 cut(s) 196, 214, 244, 307, 481
Hpy166II GTNNAC 6 cut(s) 508, 704, 849, 1118, 1168, 1297
Hpy188I TCNGA 5 cut(s) 103, 894, 1186, 1257, 1402
Hpy188III TCNNGA 5 cut(s) 322, 521, 529, 719, 1000
Hpy8I GTNNAC 6 cut(s) 508, 704, 849, 1118, 1168, 1297
Hpy99I CGWCG 5 cut(s) 352, 374, 509, 512, 620
HpyAV CCTTC 2 cut(s) 25, 328
HpyCH4III ACNGT 5 cut(s) 793, 874, 1434, 1468, 1515
HpyCH4IV ACGT 6 cut(s) 164, 312, 453, 697, 845, 1006
HpyCH4V TGCA 6 cut(s) 379, 633, 688, 974, 1133, 1178
HpyF10VI GCNNNNNNNGC 1 cut(s) 1327
HpyF3I CTNAG 4 cut(s) 62, 1226, 1369, 1419
HpySE526I ACGT 6 cut(s) 164, 312, 453, 697, 845, 1006
Hsp92I GRCGYC 3 cut(s) 234, 249, 369
Hsp92II CATG 2 cut(s) 1354, 1393
HspAI GCGC 2 cut(s) 234, 393
KasI GGCGCC 1 cut(s) 233
KroI GCCGGC 2 cut(s) 603, 670
KroNI GCCGGC 2 cut(s) 605, 672
Kzo9I GATC 3 cut(s) 351, 432, 486
LguI GCTCTTC 2 cut(s) 197, 1081
LmnI GCTCC 1 cut(s) 442
Lsp1109I GCAGC 2 cut(s) 278, 405
LweI GCATC 3 cut(s) 794, 817, 1301
MaeI CTAG 2 cut(s) 801, 927
MaeII ACGT 6 cut(s) 164, 312, 453, 697, 845, 1006
MaeIII GTNAC 6 cut(s) 65, 220, 250, 313, 874, 1105
MalI GATC 3 cut(s) 353, 434, 488
MbiI CCGCTC 2 cut(s) 212, 445
MboI GATC 3 cut(s) 351, 432, 486
MhlI GDGCHC 2 cut(s) 96, 192
MluCI AATT 5 cut(s) 729, 880, 1180, 1424, 1489
Mly113I GGCGCC 1 cut(s) 234
MlyI GAGTC 2 cut(s) 391, 510
MmeI TCCRAC 1 cut(s) 57
MroNI GCCGGC 2 cut(s) 603, 670
MroXI GAANNNNTTC 1 cut(s) 536
MseI TTAA 6 cut(s) 24, 869, 942, 1047, 1362, 1488
MslI CAYNNNNRTG 1 cut(s) 1481
MspA1I CMGCKG 3 cut(s) 291, 567, 1340
MspI CCGG 7 cut(s) 240, 366, 492, 579, 604, 671, 819
MspR9I CCNGG 5 cut(s) 515, 580, 610, 1078, 1308
MvaI CCWGG 4 cut(s) 515, 610, 1078, 1308
MvnI CGCG 4 cut(s) 347, 395, 415, 1122
MwoI GCNNNNNNNGC 1 cut(s) 1327
NaeI GCCGGC 2 cut(s) 605, 672
NarI GGCGCC 1 cut(s) 234
NciI CCSGG 1 cut(s) 580
NdeI CATATG 1 cut(s) 1129
NdeII GATC 3 cut(s) 351, 432, 486
NgoMIV GCCGGC 2 cut(s) 603, 670
NlaIII CATG 2 cut(s) 1354, 1393
NlaIV GGNNCC 3 cut(s) 235, 334, 856
NmeAIII GCCGAG 2 cut(s) 384, 490
NmuCI GTSAC 4 cut(s) 220, 250, 313, 1105
PciSI GCTCTTC 2 cut(s) 197, 1081
PdiI GCCGGC 2 cut(s) 605, 672
PdmI GAANNNNTTC 1 cut(s) 536
PflFI GACNNNGTC 1 cut(s) 248
PflMI CCANNNNNTGG 1 cut(s) 1321
PkrI GCNGC 7 cut(s) 211, 293, 296, 410, 417, 420, 497
Ple19I CGATCG 1 cut(s) 435
PleI GAGTC 2 cut(s) 391, 509
PluTI GGCGCC 1 cut(s) 237
PpsI GAGTC 2 cut(s) 391, 509
Ppu21I YACGTR 2 cut(s) 454, 1007
PshBI ATTAAT 2 cut(s) 1047, 1362
PsiI TTATAA 1 cut(s) 864
Psp124BI GAGCTC 1 cut(s) 192
Psp6I CCWGG 4 cut(s) 513, 608, 1076, 1306
PspFI CCCAGC 1 cut(s) 991
PspGI CCWGG 4 cut(s) 513, 608, 1076, 1306
PspN4I GGNNCC 3 cut(s) 235, 334, 856
PspPI GGNCC 6 cut(s) 576, 606, 854, 894, 1272, 1398
PstI CTGCAG 1 cut(s) 635
PsyI GACNNNGTC 1 cut(s) 248
PvuI CGATCG 1 cut(s) 435
PvuII CAGCTG 3 cut(s) 291, 567, 1340
RsaI GTAC 5 cut(s) 50, 452, 696, 848, 1358
RsaNI GTAC 5 cut(s) 49, 451, 695, 847, 1357
RseI CAYNNNNRTG 1 cut(s) 1481
SacI GAGCTC 1 cut(s) 192
SalI GTCGAC 1 cut(s) 506
SapI GCTCTTC 2 cut(s) 197, 1081
SaqAI TTAA 6 cut(s) 24, 869, 942, 1047, 1362, 1488
SatI GCNGC 7 cut(s) 210, 292, 295, 409, 416, 419, 496
Sau3AI GATC 3 cut(s) 351, 432, 486
Sau96I GGNCC 6 cut(s) 576, 606, 854, 894, 1272, 1398
SchI GAGTC 2 cut(s) 391, 510
ScrFI CCNGG 5 cut(s) 515, 580, 610, 1078, 1308
SduI GDGCHC 2 cut(s) 96, 192
SfaNI GCATC 3 cut(s) 794, 817, 1301
SfcI CTRYAG 3 cut(s) 631, 1261, 1300
SfoI GGCGCC 1 cut(s) 235
SgrAI CRCCGGYG 1 cut(s) 491
SgrDI CGTCGACG 1 cut(s) 506
SinI GGWCC 4 cut(s) 576, 894, 1272, 1398
SmiMI CAYNNNNRTG 1 cut(s) 1481
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 5 cut(s) 729, 880, 1180, 1424, 1489
SspDI GGCGCC 1 cut(s) 233
SspI AATATT 2 cut(s) 655, 1384
SspMI CTAG 2 cut(s) 801, 927
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 5 cut(s) 513, 578, 608, 1076, 1306
StyI CCWWGG 2 cut(s) 1315, 1446
TaaI ACNGT 5 cut(s) 793, 874, 1434, 1468, 1515
TaiI ACGT 6 cut(s) 167, 315, 456, 700, 848, 1009
TaqI TCGA 7 cut(s) 186, 273, 285, 435, 507, 540, 813
TasI AATT 5 cut(s) 729, 880, 1180, 1424, 1489
TatI WGTACW 1 cut(s) 1356
TauI GCSGC 5 cut(s) 212, 297, 411, 418, 498
Tru1I TTAA 6 cut(s) 24, 869, 942, 1047, 1362, 1488
Tru9I TTAA 6 cut(s) 24, 869, 942, 1047, 1362, 1488
TscAI CASTG 4 cut(s) 856, 928, 1180, 1439
TseFI GTSAC 4 cut(s) 220, 250, 313, 1105
TseI GCWGC 2 cut(s) 291, 418
Tsp45I GTSAC 4 cut(s) 220, 250, 313, 1105
TspDTI ATGAA 5 cut(s) 613, 1116, 1231, 1336, 1484
TspGWI ACGGA 2 cut(s) 208, 1285
TspRI CASTG 4 cut(s) 856, 928, 1180, 1439
Tth111I GACNNNGTC 1 cut(s) 248
Van91I CCANNNNNTGG 1 cut(s) 1321
VpaK11BI GGWCC 4 cut(s) 576, 894, 1272, 1398
VspI ATTAAT 2 cut(s) 1047, 1362
XapI RAATTY 1 cut(s) 1180
XcmI CCANNNNNNNNNTGG 1 cut(s) 1007
XmiI GTMKAC 2 cut(s) 507, 1296
XmnI GAANNNNTTC 1 cut(s) 536
XspI CTAG 2 cut(s) 801, 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.