MD12G1064100.v1.1
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013425
Physical Location & Seq
Forward (+)
7457600 .. 7461099
3500 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1064100.v1.1.491

Sequence Viewer

Length: 1440 bp
ATGGCAATTGCTCCACCACCTCCTCCGCCCCTCCTCATCCAATCACTCACTCTCCCGCTCCTATTCCTCCTCTTTCTACTTCCTCCCCTCCAATCCCACTTCGCCACGTCACCCACCATCGAACTCCCCAACAACCCCACCACCACCATCTCCGTCGTCGACTTCGGCGCCGTCGGCGACGGCCTCCGCTACGACACCATCGCCATCCAGTCCGCCATCGACAGCTGCCCCTCCCCCTCCCCCTGTCGCGTCATCTTCCCCGCGGGCCTCATCTACCTCACCGCGACCATCCGCCTCAAATCCGGCGTCGTATTAGACATCCAAGAAGGCGCGACACTCCTGGGCGGCACCAGGCTCGAGGACTACCCGAAGGAGCAGAGCAAGTGGTACGTGGTGCTGGCGGAGAACGCGATCAACGTCGGAATCAGCGGCGGCGGAGTGGTGGACGGTCAGGGGTTGAAGTTCGTGATGAGGTTCGATGAGAGGAAGAATGTGATGGTGAGCTGGAATCGGACCGGGGCTTGCTTGGGCGACGAGTGCCGTCCCAGGCTCGTCGGCTTCATCAATTGCCGGAATGTCAACCTCTCCAATTTCAAGCTGCGTCAGCCCGCTTACTGGTGCTTGCACATTGTACAGTGTGAGAATATTTCCATTGAAGATGTTTCAATTTATGGAGATTTCAATACACCCAACAATGATGGGATAGATATCGAGGATTCAAATAACACAGTCATTACCAGATGCCATATCAATACTGGAGATGATGCTATCTGTCCCAAGACGTACACCGGCCCCCTTTATAACTTGACTGTAACAAATTGTTGGATTCGAACAAAGTCTTCAGCAATCAAACTTGGCAGCGCTAGTTGGTTCGATTTCAAGGGTCTGGTGTTCGACAACATCACCATTGTGGATTCTCATAGAGGGCTGGGACTCCAAATACGCGATGGAGGAAATGTGAGTGACATTACCTTCTCAAACATAAATATGATCACAAGATACTATGATCAATCCTGGTGGGGAAGAGCTGAGCCAATCTACGTGACCTCATGTCCTCGACACTCAAAGTCAAAGGAGGGTTCAATCTCTAACCTACTCTTCGTTAATATCACCTCAACTTCCGAGAATGGAGTATTCTTATCAGGTTCTAAAGGTGGGCTTCTCAGTGATTTACGATTCATTAACTTGGATCTGACTTACAGAAGATGGACCAACAATGCTGGTGGGTTGGTAGACTACAGACCGGGATGCCAGGGACTTGTTAAACACATCACGGCCGGGATCATCATGGAACACATCAATGGCTTGGTGATTGACAATGTTAATATGAGATGGTCCGATGAGCCGTTAAGGCAGTGGAATGATCCTTTGGATTTCAAGTCTGCTACTGTAAATAACATATCTCTGCTTAATTTCCACTCCGGCTTGTACAAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

480

Amino Acids

52.96

Weight (kDa)

6.08

Isoelectric Point (pI)

46.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 50 - 105 1.3e-07 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 114 - 400 1.8e-24 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013425)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57790 AT3G57790
fragaria_vesca FvH4_6g22970
malus_domestica MD03G1162500.v1.1 MD12G1064100.v1.1
prunus_persica Prupe.7G070000_v2.0.a1
pyrus_communis pycom12g05590
rosa_chinensis RchiOBHm_Chr3g0480761
rosa_laevigata RLG00000023454
rosa_multiflora Rmu_co8289587.1_g000001 Rmu_sc0003902.1_g000012
rosa_roxburghii Rroxscaffold_6G00401090
rosa_rugosa Rorug03G0185600
rosa_samantha Rh3AG237700 Rh3BG271100 Rh3CG267200 Rh3DG263200
rosa_wichuraiana Rw3G021290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 801
AccB1I GGYRCC 2 cut(s) 167, 347
AccBSI CCGCTC 1 cut(s) 58
AccI GTMKAC 2 cut(s) 159, 1233
AccII CGCG 6 cut(s) 249, 263, 284, 332, 410, 945
AclWI GGATC 3 cut(s) 1197, 1289, 1358
AcoI YGGCCR 1 cut(s) 1275
AcuI CTGAAG 1 cut(s) 825
AcyI GRCGYC 2 cut(s) 168, 306
AfaI GTAC 4 cut(s) 389, 633, 785, 1430
AfeI AGCGCT 1 cut(s) 862
AfiI CCNNNNNNNGG 1 cut(s) 615
AgsI TTSAA 9 cut(s) 460, 595, 656, 666, 682, 720, 880, 1083, 1378
AhdI GACNNNNNGTC 1 cut(s) 1050
AjiI CACGTC 1 cut(s) 108
AjnI CCWGG 5 cut(s) 339, 350, 545, 1013, 1251
AjuI GAANNNNNNNTTGG 2 cut(s) 1352, 1384
AleI CACNNNNGTG 1 cut(s) 908
AluBI AGCT 4 cut(s) 225, 504, 598, 1028
AluI AGCT 4 cut(s) 225, 504, 598, 1028
AlwI GGATC 3 cut(s) 1197, 1289, 1358
Ama87I CYCGRG 1 cut(s) 356
Aor51HI AGCGCT 1 cut(s) 862
AoxI GGCC 4 cut(s) 181, 265, 790, 1275
ApeKI GCWGC 3 cut(s) 225, 598, 858
ArsI GACNNNNNNTTYG 2 cut(s) 291, 323
AspLEI GCGC 3 cut(s) 170, 332, 863
AspS9I GGNCC 5 cut(s) 265, 513, 791, 1209, 1335
AsuC2I CCSGG 3 cut(s) 517, 1245, 1279
AsuHPI GGTGA 6 cut(s) 102, 271, 511, 895, 1102, 1321
AsuII TTCGAA 1 cut(s) 829
AvaI CYCGRG 1 cut(s) 356
AvaII GGWCC 3 cut(s) 513, 1209, 1335
BanI GGYRCC 2 cut(s) 167, 347
BbsI GAAGAC 1 cut(s) 831
BbvI GCAGC 3 cut(s) 212, 585, 870
BceAI ACGGC 5 cut(s) 155, 196, 525, 1290, 1330
BcgI CGANNNNNNTGC 2 cut(s) 337, 371
BciT130I CCWGG 5 cut(s) 341, 352, 547, 1015, 1253
BclI TGATCA 2 cut(s) 990, 1006
BcnI CCSGG 3 cut(s) 517, 1245, 1279
BfaI CTAG 1 cut(s) 864
BfmI CTRYAG 1 cut(s) 1237
BfoI RGCGCY 2 cut(s) 171, 864
BglI GCCNNNNNGGC 1 cut(s) 1351
BisI GCNGC 6 cut(s) 226, 346, 430, 433, 599, 859
BlpI GCTNAGC 1 cut(s) 1029
BlsI GCNGC 6 cut(s) 227, 347, 431, 434, 600, 860
Bme1390I CCNGG 8 cut(s) 341, 352, 517, 547, 1015, 1245, 1253, 1279
Bme18I GGWCC 3 cut(s) 513, 1209, 1335
BmeRI GACNNNNNGTC 1 cut(s) 1050
BmeT110I CYCGRG 1 cut(s) 356
BmgBI CACGTC 1 cut(s) 108
BmgT120I GGNCC 5 cut(s) 265, 513, 791, 1209, 1335
BmiI GGNNCC 3 cut(s) 169, 349, 793
BmrFI CCNGG 8 cut(s) 341, 352, 517, 547, 1015, 1245, 1253, 1279
BmsI GCATC 3 cut(s) 731, 754, 1238
BpiI GAAGAC 1 cut(s) 831
BpmI CTGGAG 1 cut(s) 777
Bpu1102I GCTNAGC 1 cut(s) 1029
Bpu14I TTCGAA 1 cut(s) 829
BpuMI CCSGG 3 cut(s) 517, 1245, 1279
BsaAI YACGTR 2 cut(s) 391, 1042
BsaBI GATNNNNATC 1 cut(s) 707
BsaHI GRCGYC 2 cut(s) 168, 306
BsaJI CCNNGG 5 cut(s) 261, 340, 516, 545, 1252
BsaXI ACNNNNNCTCC 4 cut(s) 36, 66, 942, 972
Bsc4I CCNNNNNNNGG 1 cut(s) 615
Bse118I RCCGGY 1 cut(s) 788
Bse1I ACTGG 3 cut(s) 208, 620, 760
Bse8I GATNNNNATC 1 cut(s) 707
BseBI CCWGG 5 cut(s) 341, 352, 547, 1015, 1253
BseDI CCNNGG 5 cut(s) 261, 340, 516, 545, 1252
BseGI GGATG 5 cut(s) 36, 204, 288, 318, 1253
BseJI GATNNNNATC 1 cut(s) 707
BseLI CCNNNNNNNGG 1 cut(s) 615
BseMII CTCAG 2 cut(s) 1020, 1177
BseNI ACTGG 3 cut(s) 208, 620, 760
BseRI GAGGAG 3 cut(s) 12, 23, 59
BseX3I CGGCCG 1 cut(s) 1275
BseXI GCAGC 3 cut(s) 212, 585, 870
BseYI CCCAGC 1 cut(s) 928
Bsh1236I CGCG 6 cut(s) 249, 263, 284, 332, 410, 945
Bsh1285I CGRYCG 1 cut(s) 1278
BshFI GGCC 4 cut(s) 183, 267, 792, 1277
BshNI GGYRCC 2 cut(s) 167, 347
BsiEI CGRYCG 1 cut(s) 1278
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 7 cut(s) 303, 516, 571, 789, 1244, 1278, 1422
BslFI GGGAC 4 cut(s) 528, 759, 945, 1269
BslI CCNNNNNNNGG 1 cut(s) 615
BsmFI GGGAC 4 cut(s) 528, 759, 945, 1269
BsnI GGCC 4 cut(s) 183, 267, 792, 1277
BsoBI CYCGRG 1 cut(s) 356
Bsp119I TTCGAA 1 cut(s) 829
Bsp1407I TGTACA 2 cut(s) 631, 1428
Bsp143I GATC 6 cut(s) 411, 990, 1006, 1189, 1281, 1363
Bsp1720I GCTNAGC 1 cut(s) 1029
BspANI GGCC 4 cut(s) 183, 267, 792, 1277
BspCNI CTCAG 2 cut(s) 1021, 1176
BspFNI CGCG 6 cut(s) 249, 263, 284, 332, 410, 945
BspLI GGNNCC 3 cut(s) 169, 349, 793
BspPI GGATC 3 cut(s) 1197, 1289, 1358
BspQI GCTCTTC 1 cut(s) 1018
BspT104I TTCGAA 1 cut(s) 829
BspT107I GGYRCC 2 cut(s) 167, 347
BsrBI CCGCTC 1 cut(s) 58
BsrFI RCCGGY 1 cut(s) 788
BsrGI TGTACA 2 cut(s) 631, 1428
BsrI ACTGG 3 cut(s) 208, 620, 760
BssAI RCCGGY 1 cut(s) 788
BssECI CCNNGG 5 cut(s) 261, 340, 516, 545, 1252
BssMI GATC 6 cut(s) 411, 990, 1006, 1189, 1281, 1363
BssNI GRCGYC 2 cut(s) 168, 306
Bst2UI CCWGG 5 cut(s) 341, 352, 547, 1015, 1253
Bst4CI ACNGT 5 cut(s) 449, 636, 730, 811, 1390
Bst6I CTCTTC 2 cut(s) 1018, 1103
BstACI GRCGYC 2 cut(s) 168, 306
BstAUI TGTACA 2 cut(s) 631, 1428
BstBAI YACGTR 2 cut(s) 391, 1042
BstBI TTCGAA 1 cut(s) 829
BstC8I GCNNGC 5 cut(s) 265, 399, 523, 609, 623
BstDEI CTNAG 2 cut(s) 1029, 1163
BstDSI CCRYGG 1 cut(s) 261
BstF5I GGATG 5 cut(s) 36, 204, 288, 318, 1253
BstFNI CGCG 6 cut(s) 249, 263, 284, 332, 410, 945
BstH2I RGCGCY 2 cut(s) 171, 864
BstHHI GCGC 3 cut(s) 170, 332, 863
BstKTI GATC 6 cut(s) 414, 993, 1009, 1192, 1284, 1366
BstMBI GATC 6 cut(s) 411, 990, 1006, 1189, 1281, 1363
BstMCI CGRYCG 1 cut(s) 1278
BstMWI GCNNNNNNNGC 5 cut(s) 174, 407, 537, 604, 1351
BstNI CCWGG 5 cut(s) 341, 352, 547, 1015, 1253
BstSCI CCNGG 8 cut(s) 339, 350, 515, 545, 1013, 1243, 1251, 1277
BstSFI CTRYAG 1 cut(s) 1237
BstUI CGCG 6 cut(s) 249, 263, 284, 332, 410, 945
BstV1I GCAGC 3 cut(s) 212, 585, 870
BstV2I GAAGAC 1 cut(s) 831
BstX2I RGATCY 1 cut(s) 1189
BstYI RGATCY 1 cut(s) 1189
BstZI CGGCCG 1 cut(s) 1275
BsuRI GGCC 4 cut(s) 183, 267, 792, 1277
BtgI CCRYGG 1 cut(s) 261
BtgZI GCGATG 2 cut(s) 184, 960
BtrI CACGTC 1 cut(s) 108
BtsCI GGATG 5 cut(s) 36, 204, 288, 318, 1253
BtsI GCAGTG 1 cut(s) 1361
BtsIMutI CAGTG 3 cut(s) 641, 1171, 1361
Cac8I GCNNGC 5 cut(s) 265, 399, 523, 609, 623
CfoI GCGC 3 cut(s) 170, 332, 863
Cfr10I RCCGGY 1 cut(s) 788
Cfr13I GGNCC 5 cut(s) 265, 513, 791, 1209, 1335
Cfr42I CCGCGG 1 cut(s) 264
CpoI CGGWCCG 1 cut(s) 513
CseI GACGC 3 cut(s) 238, 295, 590
Csp6I GTAC 4 cut(s) 388, 632, 784, 1429
CspCI CAANNNNNGTGG 4 cut(s) 998, 1033, 1204, 1239
CspI CGGWCCG 1 cut(s) 513
CviAII CATG 2 cut(s) 1050, 1288
CviQI GTAC 4 cut(s) 388, 632, 784, 1429
DdeI CTNAG 2 cut(s) 1029, 1163
DinI GGCGCC 1 cut(s) 169
DpnI GATC 6 cut(s) 413, 992, 1008, 1191, 1283, 1365
DpnII GATC 6 cut(s) 411, 990, 1006, 1189, 1281, 1363
DriI GACNNNNNGTC 1 cut(s) 1050
EaeI YGGCCR 1 cut(s) 1275
EagI CGGCCG 1 cut(s) 1275
Eam1104I CTCTTC 2 cut(s) 1018, 1103
Eam1105I GACNNNNNGTC 1 cut(s) 1050
EarI CTCTTC 2 cut(s) 1018, 1103
EciI GGCGGA 5 cut(s) 15, 202, 281, 416, 450
EclXI CGGCCG 1 cut(s) 1275
Eco32I GATATC 1 cut(s) 709
Eco47I GGWCC 3 cut(s) 513, 1209, 1335
Eco47III AGCGCT 1 cut(s) 862
Eco52I CGGCCG 1 cut(s) 1275
Eco57I CTGAAG 1 cut(s) 825
Eco88I CYCGRG 1 cut(s) 356
EcoRII CCWGG 5 cut(s) 339, 350, 545, 1013, 1251
EcoRV GATATC 1 cut(s) 709
EgeI GGCGCC 1 cut(s) 169
EheI GGCGCC 1 cut(s) 169
FaeI CATG 2 cut(s) 1053, 1291
FalI AAGNNNNNCTT 2 cut(s) 1143, 1175
FaqI GGGAC 4 cut(s) 528, 759, 945, 1269
FatI CATG 2 cut(s) 1049, 1287
FauI CCCGC 4 cut(s) 63, 256, 268, 616
FbaI TGATCA 2 cut(s) 990, 1006
FblI GTMKAC 2 cut(s) 159, 1233
Fnu4HI GCNGC 6 cut(s) 226, 346, 430, 433, 599, 859
FokI GGATG 5 cut(s) 23, 191, 275, 305, 1260
Fsp4HI GCNGC 6 cut(s) 226, 346, 430, 433, 599, 859
FspBI CTAG 1 cut(s) 864
GlaI GCGC 3 cut(s) 169, 331, 862
GluI GCNGC 6 cut(s) 226, 346, 430, 433, 599, 859
GsaI CCCAGC 1 cut(s) 932
GsuI CTGGAG 1 cut(s) 777
HaeII RGCGCY 2 cut(s) 171, 864
HaeIII GGCC 4 cut(s) 183, 267, 792, 1277
HapII CCGG 7 cut(s) 303, 516, 571, 789, 1244, 1278, 1422
HgaI GACGC 3 cut(s) 238, 295, 590
HhaI GCGC 3 cut(s) 170, 332, 863
Hin1I GRCGYC 2 cut(s) 168, 306
Hin1II CATG 2 cut(s) 1053, 1291
Hin6I GCGC 3 cut(s) 168, 330, 861
HinP1I GCGC 3 cut(s) 168, 330, 861
HincII GTYRAC 2 cut(s) 160, 580
HindII GTYRAC 2 cut(s) 160, 580
HinfI GANTC 7 cut(s) 423, 508, 716, 826, 914, 933, 1176
HpaII CCGG 7 cut(s) 303, 516, 571, 789, 1244, 1278, 1422
HphI GGTGA 6 cut(s) 102, 271, 511, 895, 1102, 1321
Hpy166II GTNNAC 5 cut(s) 160, 445, 580, 786, 1234
Hpy188I TCNGA 5 cut(s) 422, 513, 1123, 1194, 1339
Hpy188III TCNNGA 1 cut(s) 466
Hpy8I GTNNAC 5 cut(s) 160, 445, 580, 786, 1234
Hpy99I CGWCG 8 cut(s) 158, 161, 176, 182, 311, 422, 536, 557
HpyAV CCTTC 3 cut(s) 320, 364, 982
HpyCH4III ACNGT 5 cut(s) 449, 636, 730, 811, 1390
HpyCH4IV ACGT 5 cut(s) 107, 390, 417, 782, 1041
HpyCH4V TGCA 1 cut(s) 625
HpyF10VI GCNNNNNNNGC 5 cut(s) 174, 407, 537, 604, 1351
HpyF3I CTNAG 2 cut(s) 1029, 1163
HpySE526I ACGT 5 cut(s) 107, 390, 417, 782, 1041
Hsp92I GRCGYC 2 cut(s) 168, 306
Hsp92II CATG 2 cut(s) 1053, 1291
HspAI GCGC 3 cut(s) 168, 330, 861
KasI GGCGCC 1 cut(s) 167
Ksp22I TGATCA 2 cut(s) 990, 1006
KspI CCGCGG 1 cut(s) 264
Kzo9I GATC 6 cut(s) 411, 990, 1006, 1189, 1281, 1363
LguI GCTCTTC 1 cut(s) 1018
LmnI GCTCC 3 cut(s) 16, 63, 373
Lsp1109I GCAGC 3 cut(s) 212, 585, 870
LweI GCATC 3 cut(s) 731, 754, 1238
MaeI CTAG 1 cut(s) 864
MaeII ACGT 5 cut(s) 107, 390, 417, 782, 1041
MaeIII GTNAC 4 cut(s) 108, 811, 962, 1042
MalI GATC 6 cut(s) 413, 992, 1008, 1191, 1283, 1365
MbiI CCGCTC 1 cut(s) 58
MboI GATC 6 cut(s) 411, 990, 1006, 1189, 1281, 1363
MboII GAAGA 7 cut(s) 247, 499, 668, 831, 1035, 1090, 1215
MfeI CAATTG 2 cut(s) 6, 565
MflI RGATCY 1 cut(s) 1189
MluCI AATT 6 cut(s) 6, 565, 589, 666, 817, 1411
Mly113I GGCGCC 1 cut(s) 168
MlyI GAGTC 1 cut(s) 927
MmeI TCCRAC 2 cut(s) 400, 803
MseI TTAA 6 cut(s) 1104, 1182, 1263, 1323, 1349, 1410
MslI CAYNNNNRTG 3 cut(s) 908, 986, 1299
MspA1I CMGCKG 3 cut(s) 225, 263, 429
MspI CCGG 7 cut(s) 303, 516, 571, 789, 1244, 1278, 1422
MspR9I CCNGG 8 cut(s) 341, 352, 517, 547, 1015, 1245, 1253, 1279
MunI CAATTG 2 cut(s) 6, 565
MvaI CCWGG 5 cut(s) 341, 352, 547, 1015, 1253
MvnI CGCG 6 cut(s) 249, 263, 284, 332, 410, 945
MwoI GCNNNNNNNGC 5 cut(s) 174, 407, 537, 604, 1351
NarI GGCGCC 1 cut(s) 168
NciI CCSGG 3 cut(s) 517, 1245, 1279
NdeII GATC 6 cut(s) 411, 990, 1006, 1189, 1281, 1363
NlaIII CATG 2 cut(s) 1053, 1291
NlaIV GGNNCC 3 cut(s) 169, 349, 793
NmuCI GTSAC 3 cut(s) 108, 962, 1042
NspV TTCGAA 1 cut(s) 829
OliI CACNNNNGTG 1 cut(s) 908
PaeR7I CTCGAG 1 cut(s) 356
PciSI GCTCTTC 1 cut(s) 1018
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PfeI GAWTC 6 cut(s) 423, 508, 716, 826, 914, 1176
PkrI GCNGC 6 cut(s) 227, 347, 431, 434, 600, 860
PleI GAGTC 1 cut(s) 927
PluTI GGCGCC 1 cut(s) 171
PpsI GAGTC 1 cut(s) 927
Ppu21I YACGTR 2 cut(s) 391, 1042
PsiI TTATAA 1 cut(s) 801
Psp6I CCWGG 5 cut(s) 339, 350, 545, 1013, 1251
PspFI CCCAGC 1 cut(s) 928
PspGI CCWGG 5 cut(s) 339, 350, 545, 1013, 1251
PspN4I GGNNCC 3 cut(s) 169, 349, 793
PspPI GGNCC 5 cut(s) 265, 513, 791, 1209, 1335
PspXI VCTCGAGB 1 cut(s) 356
PsuI RGATCY 1 cut(s) 1189
PvuII CAGCTG 1 cut(s) 225
RsaI GTAC 4 cut(s) 389, 633, 785, 1430
RsaNI GTAC 4 cut(s) 388, 632, 784, 1429
RseI CAYNNNNRTG 3 cut(s) 908, 986, 1299
Rsr2I CGGWCCG 1 cut(s) 513
RsrII CGGWCCG 1 cut(s) 513
SacII CCGCGG 1 cut(s) 264
SalI GTCGAC 1 cut(s) 158
SapI GCTCTTC 1 cut(s) 1018
SaqAI TTAA 6 cut(s) 1104, 1182, 1263, 1323, 1349, 1410
SatI GCNGC 6 cut(s) 226, 346, 430, 433, 599, 859
Sau3AI GATC 6 cut(s) 411, 990, 1006, 1189, 1281, 1363
Sau96I GGNCC 5 cut(s) 265, 513, 791, 1209, 1335
SchI GAGTC 1 cut(s) 927
ScrFI CCNGG 8 cut(s) 341, 352, 517, 547, 1015, 1245, 1253, 1279
SfaNI GCATC 3 cut(s) 731, 754, 1238
SfcI CTRYAG 1 cut(s) 1237
SfoI GGCGCC 1 cut(s) 169
Sfr274I CTCGAG 1 cut(s) 356
Sfr303I CCGCGG 1 cut(s) 264
SfuI TTCGAA 1 cut(s) 829
SgrBI CCGCGG 1 cut(s) 264
SinI GGWCC 3 cut(s) 513, 1209, 1335
SlaI CTCGAG 1 cut(s) 356
SmiMI CAYNNNNRTG 3 cut(s) 908, 986, 1299
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 6 cut(s) 6, 565, 589, 666, 817, 1411
SspDI GGCGCC 1 cut(s) 167
SspI AATATT 1 cut(s) 646
SspMI CTAG 1 cut(s) 864
StyD4I CCNGG 8 cut(s) 339, 350, 515, 545, 1013, 1243, 1251, 1277
TaaI ACNGT 5 cut(s) 449, 636, 730, 811, 1390
TaiI ACGT 5 cut(s) 110, 393, 420, 785, 1044
TasI AATT 6 cut(s) 6, 565, 589, 666, 817, 1411
TatI WGTACW 2 cut(s) 631, 1428
TauI GCSGC 3 cut(s) 348, 432, 435
TfiI GAWTC 6 cut(s) 423, 508, 716, 826, 914, 1176
Tru1I TTAA 6 cut(s) 1104, 1182, 1263, 1323, 1349, 1410
Tru9I TTAA 6 cut(s) 1104, 1182, 1263, 1323, 1349, 1410
TscAI CASTG 3 cut(s) 641, 1171, 1361
TseFI GTSAC 3 cut(s) 108, 962, 1042
TseI GCWGC 3 cut(s) 225, 598, 858
Tsp45I GTSAC 3 cut(s) 108, 962, 1042
TspDTI ATGAA 2 cut(s) 550, 1168
TspGWI ACGGA 1 cut(s) 142
TspRI CASTG 3 cut(s) 641, 1171, 1361
VpaK11BI GGWCC 3 cut(s) 513, 1209, 1335
XcmI CCANNNNNNNNNTGG 2 cut(s) 752, 944
XhoI CTCGAG 1 cut(s) 356
XmiI GTMKAC 2 cut(s) 159, 1233
XspI CTAG 1 cut(s) 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.