MD03G1165500.v1.1

Phospholipase A2 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014189
Physical Location & Seq
Forward (+)
21950685 .. 21953327
2643 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1165500.v1.1.491

Sequence Viewer

Length: 729 bp
ATGAACCTGGGTTTTCTTGGTAATCTCCCTTGGTTTAGGGCCCAATCAAACAGCGATTTGGAATTGGAACCAAGTCTTAAACCAACAACTGCCCTTTTCGAGCAACCTAACCAAAAGGGTGGTTTTGATGTCAAGTTGTTGGGATTGTCCCTTCTTTCCTTTGTTCCATGGCTGGTGGACAATTCTAAAGATAAGATTCGAACTCCATCCACTATTAACCGCGGCTTAAGAAGGCGTGCACAGCCTCGCAGGGTGTTTGAGAATGGGGATCCCAGTACATCTTTGCGATTTAGGCCATATGTTTCTAGGGTTCCATGGCATACTGGCGTAAGAGGTTTTCTCTCTCAGCTATTTCCAAGATATGGGCATTATTGTGGACCTAATTGGTCAAGTGGGAAAGATGGAGGATCTCCTATTTGGGACAAGCGGCCAATTGATTGGTTGGATTTTTGTTGCTACTGCCATGACATTGGTTACGACAGTCACAATCAGGCTGATCTGCTGAAGGCTGATTTAGCTTTTCTGGACTGCCTTGAGAGGCCAAATATGAGTACTAAAGGAGATGCCCGCATTGCTCACCTTTATAAGACAATGTGCACCACAGGTCTCAGGAATTTACTTATTCCGTACAGAACTCATCTTTTGAAGCTGCAAGCAGGACAACCTTTGATTCAATTTGGATGGCTAAGCAATGTTAGATGGAGAGTTTGGAAATTTCAGAAAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

243

Amino Acids

27.88

Weight (kDa)

9.72

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29070 AT4G29070
fragaria_vesca FvH4_3g27811
malus_domestica MD03G1165500.v1.1
prunus_persica Prupe.4G265500_v2.0.a1
pyrus_communis pycom03g12010
rosa_chinensis RchiOBHm_Chr5g0050481
rosa_laevigata RLG00000034704
rosa_multiflora Rmu_sc0000459.1_g000004 Rmu_sc0003202.1_g000007
rosa_roxburghii Rroxscaffold_1G00030410
rosa_rugosa Rorug05G0261300
rosa_samantha Rh5AG333000 Rh5BG343100 Rh5CG370400 Rh5DG355700
rosa_wichuraiana Rw5G031460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
AccB7I CCANNNNNTGG 1 cut(s) 362
AccII CGCG 1 cut(s) 222
AciI CCGC 4 cut(s) 220, 222, 427, 568
AclWI GGATC 3 cut(s) 263, 276, 415
AcoI YGGCCR 1 cut(s) 428
AcsI RAATTY 2 cut(s) 613, 713
AcuI CTGAAG 1 cut(s) 524
AfaI GTAC 3 cut(s) 277, 553, 629
AfiI CCNNNNNNNGG 1 cut(s) 362
AflII CTTAAG 1 cut(s) 226
AgsI TTSAA 2 cut(s) 646, 674
AjnI CCWGG 1 cut(s) 6
AluBI AGCT 3 cut(s) 349, 518, 649
AluI AGCT 3 cut(s) 349, 518, 649
Alw21I GWGCWC 2 cut(s) 241, 599
Alw26I GTCTC 1 cut(s) 611
Alw44I GTGCAC 2 cut(s) 237, 595
AlwI GGATC 3 cut(s) 263, 276, 415
AoxI GGCC 4 cut(s) 39, 293, 428, 539
ApaI GGGCCC 1 cut(s) 43
ApaLI GTGCAC 2 cut(s) 237, 595
ApeKI GCWGC 1 cut(s) 649
ApoI RAATTY 2 cut(s) 613, 713
AspS9I GGNCC 3 cut(s) 39, 40, 377
AsuHPI GGTGA 1 cut(s) 569
AsuII TTCGAA 1 cut(s) 199
AvaII GGWCC 1 cut(s) 377
BaeGI GKGCMC 3 cut(s) 43, 241, 599
BamHI GGATCC 1 cut(s) 268
BanII GRGCYC 1 cut(s) 43
Bbv12I GWGCWC 2 cut(s) 241, 599
BbvI GCAGC 1 cut(s) 636
BccI CCATC 4 cut(s) 214, 395, 675, 693
BciT130I CCWGG 1 cut(s) 8
BcoDI GTCTC 1 cut(s) 611
BfaI CTAG 1 cut(s) 306
BfrI CTTAAG 1 cut(s) 226
BisI GCNGC 3 cut(s) 223, 428, 650
BlpI GCTNAGC 1 cut(s) 686
BlsI GCNGC 3 cut(s) 224, 429, 651
BmcAI AGTACT 1 cut(s) 553
Bme1390I CCNGG 1 cut(s) 8
Bme18I GGWCC 1 cut(s) 377
BmgT120I GGNCC 3 cut(s) 39, 40, 377
BmiI GGNNCC 4 cut(s) 41, 69, 270, 312
BmrFI CCNGG 1 cut(s) 8
BmrI ACTGGG 1 cut(s) 267
BmsI GCATC 1 cut(s) 553
BmuI ACTGGG 1 cut(s) 267
BplI GAGNNNNNCTC 2 cut(s) 324, 356
Bpu1102I GCTNAGC 1 cut(s) 686
Bpu14I TTCGAA 1 cut(s) 199
BpuEI CTTGAG 1 cut(s) 554
BsaI GGTCTC 1 cut(s) 611
BsaJI CCNNGG 5 cut(s) 7, 29, 167, 220, 314
Bsc4I CCNNNNNNNGG 1 cut(s) 362
Bse1I ACTGG 2 cut(s) 273, 328
Bse3DI GCAATG 2 cut(s) 570, 697
BseBI CCWGG 1 cut(s) 8
BseDI CCNNGG 5 cut(s) 7, 29, 167, 220, 314
BseGI GGATG 2 cut(s) 206, 686
BseLI CCNNNNNNNGG 1 cut(s) 362
BseMI GCAATG 2 cut(s) 570, 697
BseMII CTCAG 2 cut(s) 359, 622
BseNI ACTGG 2 cut(s) 273, 328
BseSI GKGCMC 3 cut(s) 43, 241, 599
BseXI GCAGC 1 cut(s) 636
Bsh1236I CGCG 1 cut(s) 222
BshFI GGCC 4 cut(s) 41, 295, 430, 541
BsiHKAI GWGCWC 2 cut(s) 241, 599
BslFI GGGAC 2 cut(s) 133, 434
BslI CCNNNNNNNGG 1 cut(s) 362
BsmAI GTCTC 1 cut(s) 611
BsmFI GGGAC 2 cut(s) 133, 434
BsnI GGCC 4 cut(s) 41, 295, 430, 541
Bso31I GGTCTC 1 cut(s) 611
Bsp119I TTCGAA 1 cut(s) 199
Bsp120I GGGCCC 1 cut(s) 39
Bsp1286I GDGCHC 3 cut(s) 43, 241, 599
Bsp143I GATC 3 cut(s) 268, 407, 496
Bsp1720I GCTNAGC 1 cut(s) 686
Bsp19I CCATGG 2 cut(s) 167, 314
BspACI CCGC 4 cut(s) 220, 222, 427, 568
BspANI GGCC 4 cut(s) 41, 295, 430, 541
BspCNI CTCAG 2 cut(s) 358, 621
BspFNI CGCG 1 cut(s) 222
BspLI GGNNCC 4 cut(s) 41, 69, 270, 312
BspPI GGATC 3 cut(s) 263, 276, 415
BspT104I TTCGAA 1 cut(s) 199
BspTI CTTAAG 1 cut(s) 226
BspTNI GGTCTC 1 cut(s) 611
BsrDI GCAATG 2 cut(s) 570, 697
BsrI ACTGG 2 cut(s) 273, 328
BssECI CCNNGG 5 cut(s) 7, 29, 167, 220, 314
BssMI GATC 3 cut(s) 268, 407, 496
BssT1I CCWWGG 3 cut(s) 29, 167, 314
Bst2UI CCWGG 1 cut(s) 8
Bst4CI ACNGT 1 cut(s) 482
BstAFI CTTAAG 1 cut(s) 226
BstBI TTCGAA 1 cut(s) 199
BstC8I GCNNGC 3 cut(s) 237, 568, 654
BstDEI CTNAG 3 cut(s) 345, 608, 686
BstDSI CCRYGG 3 cut(s) 167, 220, 314
BstF5I GGATG 2 cut(s) 206, 686
BstFNI CGCG 1 cut(s) 222
BstKTI GATC 3 cut(s) 271, 410, 499
BstMAI GTCTC 1 cut(s) 611
BstMBI GATC 3 cut(s) 268, 407, 496
BstMWI GCNNNNNNNGC 4 cut(s) 241, 292, 515, 572
BstNI CCWGG 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 6
BstSLI GKGCMC 3 cut(s) 43, 241, 599
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 1 cut(s) 636
BstX2I RGATCY 2 cut(s) 268, 407
BstXI CCANNNNNNTGG 3 cut(s) 119, 438, 470
BstYI RGATCY 2 cut(s) 268, 407
BsuRI GGCC 4 cut(s) 41, 295, 430, 541
BtgI CCRYGG 3 cut(s) 167, 220, 314
BtsCI GGATG 2 cut(s) 206, 686
Cac8I GCNNGC 3 cut(s) 237, 568, 654
Cfr13I GGNCC 3 cut(s) 39, 40, 377
Cfr42I CCGCGG 1 cut(s) 223
Csp6I GTAC 3 cut(s) 276, 552, 628
CviAII CATG 3 cut(s) 168, 315, 464
CviQI GTAC 3 cut(s) 276, 552, 628
DdeI CTNAG 3 cut(s) 345, 608, 686
DpnI GATC 3 cut(s) 270, 409, 498
DpnII GATC 3 cut(s) 268, 407, 496
EaeI YGGCCR 1 cut(s) 428
Eco130I CCWWGG 3 cut(s) 29, 167, 314
Eco24I GRGCYC 1 cut(s) 43
Eco31I GGTCTC 1 cut(s) 611
Eco47I GGWCC 1 cut(s) 377
Eco57I CTGAAG 1 cut(s) 524
EcoO109I RGGNCCY 1 cut(s) 39
EcoRII CCWGG 1 cut(s) 6
EcoT14I CCWWGG 3 cut(s) 29, 167, 314
EcoT38I GRGCYC 1 cut(s) 43
ErhI CCWWGG 3 cut(s) 29, 167, 314
FaeI CATG 3 cut(s) 171, 318, 467
FaiI YATR 9 cut(s) 169, 298, 300, 316, 321, 363, 465, 548, 585
FaqI GGGAC 2 cut(s) 133, 434
FatI CATG 3 cut(s) 167, 314, 463
FauI CCCGC 1 cut(s) 575
FauNDI CATATG 1 cut(s) 298
Fnu4HI GCNGC 3 cut(s) 223, 428, 650
FokI GGATG 2 cut(s) 193, 693
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 3 cut(s) 223, 428, 650
FspBI CTAG 1 cut(s) 306
GluI GCNGC 3 cut(s) 223, 428, 650
HaeIII GGCC 4 cut(s) 41, 295, 430, 541
Hin1II CATG 3 cut(s) 171, 318, 467
HinfI GANTC 2 cut(s) 196, 670
HphI GGTGA 1 cut(s) 569
Hpy166II GTNNAC 4 cut(s) 178, 239, 377, 597
Hpy188I TCNGA 1 cut(s) 720
Hpy188III TCNNGA 2 cut(s) 524, 610
Hpy8I GTNNAC 4 cut(s) 178, 239, 377, 597
HpyAV CCTTC 3 cut(s) 161, 225, 499
HpyCH4III ACNGT 1 cut(s) 482
HpyCH4V TGCA 3 cut(s) 239, 597, 652
HpyF10VI GCNNNNNNNGC 4 cut(s) 241, 292, 515, 572
HpyF3I CTNAG 3 cut(s) 345, 608, 686
Hsp92II CATG 3 cut(s) 171, 318, 467
KspI CCGCGG 1 cut(s) 223
Kzo9I GATC 3 cut(s) 268, 407, 496
Lsp1109I GCAGC 1 cut(s) 636
LweI GCATC 1 cut(s) 553
MaeI CTAG 1 cut(s) 306
MaeIII GTNAC 2 cut(s) 473, 482
MalI GATC 3 cut(s) 270, 409, 498
MboI GATC 3 cut(s) 268, 407, 496
MfeI CAATTG 1 cut(s) 432
MflI RGATCY 2 cut(s) 268, 407
MhlI GDGCHC 3 cut(s) 43, 241, 599
MluCI AATT 7 cut(s) 62, 181, 382, 432, 613, 674, 713
MmeI TCCRAC 1 cut(s) 423
MnlI CCTC 4 cut(s) 255, 326, 398, 531
MseI TTAA 3 cut(s) 78, 216, 227
MslI CAYNNNNRTG 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 222
MspCI CTTAAG 1 cut(s) 226
MspR9I CCNGG 1 cut(s) 8
MunI CAATTG 1 cut(s) 432
MvaI CCWGG 1 cut(s) 8
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 4 cut(s) 241, 292, 515, 572
NcoI CCATGG 2 cut(s) 167, 314
NdeI CATATG 1 cut(s) 298
NdeII GATC 3 cut(s) 268, 407, 496
NlaIII CATG 3 cut(s) 171, 318, 467
NlaIV GGNNCC 4 cut(s) 41, 69, 270, 312
NmuCI GTSAC 1 cut(s) 482
NspV TTCGAA 1 cut(s) 199
PfeI GAWTC 2 cut(s) 196, 670
PflMI CCANNNNNTGG 1 cut(s) 362
PkrI GCNGC 3 cut(s) 224, 429, 651
PsiI TTATAA 1 cut(s) 585
Psp6I CCWGG 1 cut(s) 6
PspGI CCWGG 1 cut(s) 6
PspN4I GGNNCC 4 cut(s) 41, 69, 270, 312
PspOMI GGGCCC 1 cut(s) 39
PspPI GGNCC 3 cut(s) 39, 40, 377
PsuI RGATCY 2 cut(s) 268, 407
RsaI GTAC 3 cut(s) 277, 553, 629
RsaNI GTAC 3 cut(s) 276, 552, 628
RseI CAYNNNNRTG 1 cut(s) 372
SacII CCGCGG 1 cut(s) 223
SaqAI TTAA 3 cut(s) 78, 216, 227
SatI GCNGC 3 cut(s) 223, 428, 650
Sau3AI GATC 3 cut(s) 268, 407, 496
Sau96I GGNCC 3 cut(s) 39, 40, 377
ScaI AGTACT 1 cut(s) 553
ScrFI CCNGG 1 cut(s) 8
SduI GDGCHC 3 cut(s) 43, 241, 599
SfaNI GCATC 1 cut(s) 553
Sfr303I CCGCGG 1 cut(s) 223
SfuI TTCGAA 1 cut(s) 199
SgrBI CCGCGG 1 cut(s) 223
SinI GGWCC 1 cut(s) 377
SmiMI CAYNNNNRTG 1 cut(s) 372
SmlI CTYRAG 2 cut(s) 226, 533
SmoI CTYRAG 2 cut(s) 226, 533
Sse9I AATT 7 cut(s) 62, 181, 382, 432, 613, 674, 713
SsiI CCGC 4 cut(s) 220, 222, 427, 568
SspMI CTAG 1 cut(s) 306
StyD4I CCNGG 1 cut(s) 6
StyI CCWWGG 3 cut(s) 29, 167, 314
TaaI ACNGT 1 cut(s) 482
TaqI TCGA 2 cut(s) 99, 199
TasI AATT 7 cut(s) 62, 181, 382, 432, 613, 674, 713
TatI WGTACW 2 cut(s) 275, 551
TauI GCSGC 2 cut(s) 225, 430
TfiI GAWTC 2 cut(s) 196, 670
Tru1I TTAA 3 cut(s) 78, 216, 227
Tru9I TTAA 3 cut(s) 78, 216, 227
TseFI GTSAC 1 cut(s) 482
TseI GCWGC 1 cut(s) 649
Tsp45I GTSAC 1 cut(s) 482
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 615
Van91I CCANNNNNTGG 1 cut(s) 362
Vha464I CTTAAG 1 cut(s) 226
VneI GTGCAC 2 cut(s) 237, 595
VpaK11BI GGWCC 1 cut(s) 377
XapI RAATTY 2 cut(s) 613, 713
XspI CTAG 1 cut(s) 306
ZrmI AGTACT 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.