Rmu_sc0000459.1_g000004

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000459.1
OG0014189
Physical Location & Seq
Reverse (-)
19882 .. 21483
1602 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000459.1_g000004.1.cds

Sequence Viewer

Length: 657 bp
atgtgtaaggacgatacttgtatccagtcactagctgtaatgcagagagcttccgttggctggttcgataggtttccgactggttctggtggttcttccgccagttctggattcctgggatcgagggcttcaagttgtgtggcagaggtagaaaaggccataacaagtgtaagggtgagtcaccatcatgttgataaccagaaagtaccgacaaaagaccagaccaagacacagcagaccgaccgtgacaccaaaacaatggcatcaacacgacacagaaacaaccaaccccaaaccctcccttacaagaggagggacttattttcaccaagacaacaactaaaaacaaccaaagaaaacctaaacaaccagaaaccaaagaccaccagagagacccaagaccaaaggcccgacccaaacaccacctcccacagccaaaaccaagcgcagcaggtaaccattcccgccgccagtgagccctgtcgccaccacgccgccgccccacaacaatacgccattgcgccgaatcccttcgccgctgcacctccttgtcacgacgtagccagcccctacatcaggcaaacagccacaaaacgatcccagaccaaagcgaaggcagatcccattcgagatctccaatgcctcgatccgatctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

218

Amino Acids

24.16

Weight (kDa)

9.63

Isoelectric Point (pI)

48.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29070 AT4G29070
fragaria_vesca FvH4_3g27811
malus_domestica MD03G1165500.v1.1
prunus_persica Prupe.4G265500_v2.0.a1
pyrus_communis pycom03g12010
rosa_chinensis RchiOBHm_Chr5g0050481
rosa_laevigata RLG00000034704
rosa_multiflora Rmu_sc0000459.1_g000004 Rmu_sc0003202.1_g000007
rosa_roxburghii Rroxscaffold_1G00030410
rosa_rugosa Rorug05G0261300
rosa_samantha Rh5AG333000 Rh5BG343100 Rh5CG370400 Rh5DG355700
rosa_wichuraiana Rw5G031460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 442
AciI CCGC 6 cut(s) 99, 465, 468, 495, 498, 537
AclWI GGATC 4 cut(s) 127, 591, 614, 641
AfaI GTAC 1 cut(s) 207
AfiI CCNNNNNNNGG 2 cut(s) 60, 576
AgsI TTSAA 1 cut(s) 132
AjnI CCWGG 1 cut(s) 114
AluBI AGCT 2 cut(s) 35, 50
AluI AGCT 2 cut(s) 35, 50
Alw26I GTCTC 1 cut(s) 386
AlwI GGATC 4 cut(s) 127, 591, 614, 641
AoxI GGCC 2 cut(s) 156, 407
ApeKI GCWGC 2 cut(s) 448, 539
Asp700I GAANNNNTTC 1 cut(s) 530
AspLEI GCGC 2 cut(s) 448, 523
AspS9I GGNCC 1 cut(s) 408
AsuHPI GGTGA 3 cut(s) 173, 187, 318
BanII GRGCYC 1 cut(s) 480
BbvI GCAGC 2 cut(s) 460, 526
BccI CCATC 1 cut(s) 192
BciT130I CCWGG 1 cut(s) 116
BciVI GTATCC 1 cut(s) 32
BcoDI GTCTC 1 cut(s) 386
BfaI CTAG 1 cut(s) 32
BfuAI ACCTGC 1 cut(s) 442
BfuI GTATCC 1 cut(s) 32
BglII AGATCT 1 cut(s) 631
BisI GCNGC 6 cut(s) 449, 468, 495, 498, 537, 540
BlsI GCNGC 6 cut(s) 450, 469, 496, 499, 538, 541
Bme1390I CCNGG 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 408
BmrFI CCNGG 1 cut(s) 116
BmsI GCATC 1 cut(s) 272
BsaI GGTCTC 1 cut(s) 386
BsaJI CCNNGG 1 cut(s) 115
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 576
Bse1I ACTGG 4 cut(s) 25, 85, 102, 471
Bse3DI GCAATG 1 cut(s) 516
BseBI CCWGG 1 cut(s) 116
BseDI CCNNGG 1 cut(s) 115
BseLI CCNNNNNNNGG 2 cut(s) 60, 576
BseMI GCAATG 1 cut(s) 516
BseNI ACTGG 4 cut(s) 25, 85, 102, 471
BseRI GAGGAG 1 cut(s) 325
BseXI GCAGC 2 cut(s) 460, 526
BsgI GTGCAG 1 cut(s) 525
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 2 cut(s) 158, 409
BsiEI CGRYCG 1 cut(s) 244
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 2 cut(s) 60, 576
BsmAI GTCTC 1 cut(s) 386
BsmFI GGGAC 1 cut(s) 329
BsnI GGCC 2 cut(s) 158, 409
Bso31I GGTCTC 1 cut(s) 386
Bsp1286I GDGCHC 1 cut(s) 480
Bsp143I GATC 6 cut(s) 119, 596, 619, 631, 646, 651
BspACI CCGC 6 cut(s) 99, 465, 468, 495, 498, 537
BspANI GGCC 2 cut(s) 158, 409
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 4 cut(s) 127, 591, 614, 641
BspTNI GGTCTC 1 cut(s) 386
BsrDI GCAATG 1 cut(s) 516
BsrI ACTGG 4 cut(s) 25, 85, 102, 471
BssECI CCNNGG 1 cut(s) 115
BssMI GATC 6 cut(s) 119, 596, 619, 631, 646, 651
Bst2UI CCWGG 1 cut(s) 116
Bst4CI ACNGT 1 cut(s) 245
BstC8I GCNNGC 1 cut(s) 565
BstEII GGTNACC 1 cut(s) 454
BstENI CCTNNNNNAGG 1 cut(s) 574
BstHHI GCGC 2 cut(s) 448, 523
BstKTI GATC 6 cut(s) 122, 599, 622, 634, 649, 654
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 6 cut(s) 119, 596, 619, 631, 646, 651
BstMCI CGRYCG 1 cut(s) 244
BstNI CCWGG 1 cut(s) 116
BstPI GGTNACC 1 cut(s) 454
BstSCI CCNGG 1 cut(s) 114
BstV1I GCAGC 2 cut(s) 460, 526
BstX2I RGATCY 2 cut(s) 619, 631
BstXI CCANNNNNNTGG 1 cut(s) 259
BstYI RGATCY 2 cut(s) 619, 631
BsuI GTATCC 1 cut(s) 32
BsuRI GGCC 2 cut(s) 158, 409
BtsIMutI CAGTG 1 cut(s) 478
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 1 cut(s) 565
CfoI GCGC 2 cut(s) 448, 523
Cfr13I GGNCC 1 cut(s) 408
Csp6I GTAC 1 cut(s) 206
CspCI CAANNNNNGTGG 2 cut(s) 120, 155
CviAII CATG 1 cut(s) 188
CviQI GTAC 1 cut(s) 206
DpnI GATC 6 cut(s) 121, 598, 621, 633, 648, 653
DpnII GATC 6 cut(s) 119, 596, 619, 631, 646, 651
EciI GGCGGA 1 cut(s) 88
Eco24I GRGCYC 1 cut(s) 480
Eco31I GGTCTC 1 cut(s) 386
Eco91I GGTNACC 1 cut(s) 454
EcoNI CCTNNNNNAGG 1 cut(s) 574
EcoO65I GGTNACC 1 cut(s) 454
EcoRII CCWGG 1 cut(s) 114
EcoT38I GRGCYC 1 cut(s) 480
FaeI CATG 1 cut(s) 191
FaiI YATR 2 cut(s) 161, 189
FaqI GGGAC 1 cut(s) 329
FatI CATG 1 cut(s) 187
FauI CCCGC 1 cut(s) 472
Fnu4HI GCNGC 6 cut(s) 449, 468, 495, 498, 537, 540
FriOI GRGCYC 1 cut(s) 480
Fsp4HI GCNGC 6 cut(s) 449, 468, 495, 498, 537, 540
FspBI CTAG 1 cut(s) 32
GlaI GCGC 2 cut(s) 447, 522
GluI GCNGC 6 cut(s) 449, 468, 495, 498, 537, 540
HaeIII GGCC 2 cut(s) 158, 409
HhaI GCGC 2 cut(s) 448, 523
Hin1II CATG 1 cut(s) 191
Hin6I GCGC 2 cut(s) 446, 521
HinP1I GCGC 2 cut(s) 446, 521
HinfI GANTC 3 cut(s) 111, 178, 526
HphI GGTGA 3 cut(s) 173, 187, 318
Hpy188I TCNGA 3 cut(s) 78, 651, 656
Hpy188III TCNNGA 3 cut(s) 108, 554, 629
Hpy99I CGWCG 1 cut(s) 560
HpyAV CCTTC 2 cut(s) 541, 607
HpyCH4III ACNGT 1 cut(s) 245
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 2 cut(s) 43, 542
HpySE526I ACGT 1 cut(s) 558
Hsp92II CATG 1 cut(s) 191
HspAI GCGC 2 cut(s) 446, 521
Kzo9I GATC 6 cut(s) 119, 596, 619, 631, 646, 651
Lsp1109I GCAGC 2 cut(s) 460, 526
LweI GCATC 1 cut(s) 272
MaeI CTAG 1 cut(s) 32
MaeII ACGT 1 cut(s) 558
MaeIII GTNAC 5 cut(s) 27, 179, 245, 454, 551
MalI GATC 6 cut(s) 121, 598, 621, 633, 648, 653
MboI GATC 6 cut(s) 119, 596, 619, 631, 646, 651
MboII GAAGA 1 cut(s) 87
MflI RGATCY 2 cut(s) 619, 631
MhlI GDGCHC 1 cut(s) 480
MlyI GAGTC 1 cut(s) 187
MmeI TCCRAC 1 cut(s) 101
MnlI CCTC 8 cut(s) 117, 139, 303, 306, 308, 436, 555, 653
MroXI GAANNNNTTC 1 cut(s) 530
MslI CAYNNNNRTG 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 539
MspR9I CCNGG 1 cut(s) 116
MvaI CCWGG 1 cut(s) 116
NdeII GATC 6 cut(s) 119, 596, 619, 631, 646, 651
NlaIII CATG 1 cut(s) 191
NmuCI GTSAC 4 cut(s) 27, 179, 245, 551
PdmI GAANNNNTTC 1 cut(s) 530
PfeI GAWTC 2 cut(s) 111, 526
PkrI GCNGC 6 cut(s) 450, 469, 496, 499, 538, 541
PleI GAGTC 1 cut(s) 186
PpsI GAGTC 1 cut(s) 186
Psp6I CCWGG 1 cut(s) 114
PspEI GGTNACC 1 cut(s) 454
PspGI CCWGG 1 cut(s) 114
PspPI GGNCC 1 cut(s) 408
PsuI RGATCY 2 cut(s) 619, 631
RsaI GTAC 1 cut(s) 207
RsaNI GTAC 1 cut(s) 206
RseI CAYNNNNRTG 1 cut(s) 186
SatI GCNGC 6 cut(s) 449, 468, 495, 498, 537, 540
Sau3AI GATC 6 cut(s) 119, 596, 619, 631, 646, 651
Sau96I GGNCC 1 cut(s) 408
SchI GAGTC 1 cut(s) 187
ScrFI CCNGG 1 cut(s) 116
SduI GDGCHC 1 cut(s) 480
SetI ASST 9 cut(s) 37, 52, 74, 150, 363, 428, 456, 547, 561
SfaNI GCATC 1 cut(s) 272
SmiMI CAYNNNNRTG 1 cut(s) 186
SsiI CCGC 6 cut(s) 99, 465, 468, 495, 498, 537
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 114
TaaI ACNGT 1 cut(s) 245
TaiI ACGT 1 cut(s) 561
TaqI TCGA 4 cut(s) 66, 122, 628, 645
TaqII GACCGA 1 cut(s) 254
TauI GCSGC 4 cut(s) 470, 497, 500, 539
TfiI GAWTC 2 cut(s) 111, 526
TscAI CASTG 1 cut(s) 478
TseFI GTSAC 4 cut(s) 27, 179, 245, 551
TseI GCWGC 2 cut(s) 448, 539
Tsp45I GTSAC 4 cut(s) 27, 179, 245, 551
TspGWI ACGGA 1 cut(s) 43
TspRI CASTG 1 cut(s) 478
XagI CCTNNNNNAGG 1 cut(s) 574
XmnI GAANNNNTTC 1 cut(s) 530
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.