MD03G1180200.v1.1

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0018486
Physical Location & Seq
Forward (+)
24741906 .. 24747551
5646 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1180200.v1.1.491

Sequence Viewer

Length: 264 bp
ATGGAGGGTTTGGAGGGTCCGACGATCCGATTTTGCCAGCGCCGTCGGAGATGCTGCCTGAGGAGGGCTTCGCTCTCAGAGAATGGAGGAGGGGAAACAATAGCTTTGGGTTTCCAGCACTACATTTTGGCCTTGGGAACCGCTGTGATGATCCCCACATTCCGTGTTCCTTTGATGGGTGGTGATGATGGTGACAAAGTGAGAATAGTGCAGACTCTGCTTTTTATTCAAGGAATCAGCACACTTCTACAAACATTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Xan_ur_permease

Protein Analysis

88

Amino Acids

9.63

Weight (kDa)

9.15

Isoelectric Point (pI)

53.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 141
AclWI GGATC 2 cut(s) 19, 145
AfiI CCNNNNNNNGG 2 cut(s) 64, 176
AgsI TTSAA 1 cut(s) 230
AluBI AGCT 1 cut(s) 104
AluI AGCT 1 cut(s) 104
AlwI GGATC 2 cut(s) 19, 145
AlwNI CAGNNNCTG 1 cut(s) 217
AoxI GGCC 1 cut(s) 129
ApeKI GCWGC 1 cut(s) 54
AspLEI GCGC 1 cut(s) 42
AspS9I GGNCC 1 cut(s) 17
AsuHPI GGTGA 2 cut(s) 194, 203
AvaII GGWCC 1 cut(s) 17
AxyI CCTNAGG 1 cut(s) 59
BbvI GCAGC 1 cut(s) 41
BccI CCATC 2 cut(s) 169, 182
BceAI ACGGC 1 cut(s) 27
BfoI RGCGCY 1 cut(s) 43
BisI GCNGC 1 cut(s) 55
BlsI GCNGC 1 cut(s) 56
Bme18I GGWCC 1 cut(s) 17
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 2 cut(s) 18, 139
BmsI GCATC 1 cut(s) 41
BsaJI CCNNGG 1 cut(s) 132
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 176
Bse21I CCTNAGG 1 cut(s) 59
BseDI CCNNGG 1 cut(s) 132
BseLI CCNNNNNNNGG 2 cut(s) 64, 176
BseMII CTCAG 2 cut(s) 50, 90
BseRI GAGGAG 2 cut(s) 76, 102
BseXI GCAGC 1 cut(s) 41
BsgI GTGCAG 1 cut(s) 230
BshFI GGCC 1 cut(s) 131
BslI CCNNNNNNNGG 2 cut(s) 64, 176
BsnI GGCC 1 cut(s) 131
Bsp143I GATC 2 cut(s) 24, 150
BspACI CCGC 1 cut(s) 141
BspANI GGCC 1 cut(s) 131
BspCNI CTCAG 2 cut(s) 51, 89
BspLI GGNNCC 2 cut(s) 18, 139
BspPI GGATC 2 cut(s) 19, 145
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 2 cut(s) 24, 150
BssT1I CCWWGG 1 cut(s) 132
BstAPI GCANNNNNTGC 1 cut(s) 217
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 2 cut(s) 59, 76
BstH2I RGCGCY 1 cut(s) 43
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 2 cut(s) 27, 153
BstMBI GATC 2 cut(s) 24, 150
BstMWI GCNNNNNNNGC 1 cut(s) 217
BstV1I GCAGC 1 cut(s) 41
Bsu36I CCTNAGG 1 cut(s) 59
BsuRI GGCC 1 cut(s) 131
Cac8I GCNNGC 1 cut(s) 38
CaiI CAGNNNCTG 1 cut(s) 217
CfoI GCGC 1 cut(s) 42
Cfr13I GGNCC 1 cut(s) 17
CviJI RGCY 3 cut(s) 68, 104, 131
CviKI_1 RGCY 3 cut(s) 68, 104, 131
DdeI CTNAG 2 cut(s) 59, 76
DpnI GATC 2 cut(s) 26, 152
DpnII GATC 2 cut(s) 24, 150
Eco130I CCWWGG 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 17
Eco81I CCTNAGG 1 cut(s) 59
EcoT14I CCWWGG 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 132
Fnu4HI GCNGC 1 cut(s) 55
Fsp4HI GCNGC 1 cut(s) 55
GlaI GCGC 1 cut(s) 41
GluI GCNGC 1 cut(s) 55
HaeII RGCGCY 1 cut(s) 43
HaeIII GGCC 1 cut(s) 131
HhaI GCGC 1 cut(s) 42
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HinfI GANTC 2 cut(s) 214, 234
HphI GGTGA 2 cut(s) 194, 203
Hpy188I TCNGA 4 cut(s) 21, 29, 48, 79
Hpy99I CGWCG 2 cut(s) 25, 48
HpyCH4V TGCA 1 cut(s) 211
HpyF10VI GCNNNNNNNGC 1 cut(s) 217
HpyF3I CTNAG 2 cut(s) 59, 76
HspAI GCGC 1 cut(s) 40
Kzo9I GATC 2 cut(s) 24, 150
LpnPI CCDG 3 cut(s) 50, 71, 128
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 1 cut(s) 41
MaeIII GTNAC 1 cut(s) 191
MalI GATC 2 cut(s) 26, 152
MboI GATC 2 cut(s) 24, 150
MlyI GAGTC 1 cut(s) 208
MmeI TCCRAC 2 cut(s) 26, 44
MnlI CCTC 5 cut(s) 7, 54, 57, 80, 83
MspA1I CMGCKG 1 cut(s) 143
MwoI GCNNNNNNNGC 1 cut(s) 217
NdeII GATC 2 cut(s) 24, 150
NlaIV GGNNCC 2 cut(s) 18, 139
NmuCI GTSAC 1 cut(s) 191
PfeI GAWTC 1 cut(s) 234
PkrI GCNGC 1 cut(s) 56
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
PspN4I GGNNCC 2 cut(s) 18, 139
PspPI GGNCC 1 cut(s) 17
PstNI CAGNNNCTG 1 cut(s) 217
SatI GCNGC 1 cut(s) 55
Sau3AI GATC 2 cut(s) 24, 150
Sau96I GGNCC 1 cut(s) 17
SchI GAGTC 1 cut(s) 208
SetI ASST 1 cut(s) 106
SfaNI GCATC 1 cut(s) 41
SgeI CNNG 6 cut(s) 49, 70, 127, 145, 176, 242
SinI GGWCC 1 cut(s) 17
SsiI CCGC 1 cut(s) 141
StyI CCWWGG 1 cut(s) 132
TfiI GAWTC 1 cut(s) 234
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 1 cut(s) 54
Tsp45I GTSAC 1 cut(s) 191
TspGWI ACGGA 1 cut(s) 152
VpaK11BI GGWCC 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.