MD03G1206100.v1.1

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0016113
Physical Location & Seq
Reverse (-)
28182408 .. 28185068
2661 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1206100.v1.1.491

Sequence Viewer

Length: 450 bp
ATGGAAGCGCAGACGATGAAGAAGGAGCTGGCGGTGGCCGTGGCACTTGTTCTCTTCACAGTCTTGATCTCCGGCGTCGGAGCCTGGACTGGTGAAATCCACGGCAGAGTTGTTTGCGATGTCTGTGGCGACTCCGCCATCGGACCCGAAGATCATGTTCTAGAAGGTGCTGAGGTAGCTGTACTGTGCATTACAAAATCAGGGGAAGTTCTAAATTACCAGGCATTCACGAATGATAAGGGGGTATATACGGTGGCGGAGACAATGCCCGAGAGTGACAGGTGGGATGCATGCCTGGCACGACCTATCAGCAGCTTCCATGAGCACTGCACTCATCTCGGGGGTGGTAGCTCAGGCGTAAAGTTCACTTACAGTCACCCATCCGGCTATTCTCACACCGTTAAAACTTTCGTCTATCGACCAGCAATTGTACCGGCATACTGCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

150

Amino Acids

15.98

Weight (kDa)

5.55

Isoelectric Point (pI)

32.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 33 - 116 5.1e-08 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016113)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54855
fragaria_vesca FvH4_3g22360
malus_domestica MD03G1206100.v1.1 MD11G1221600.v1.1
prunus_persica Prupe.4G210000_v2.0.a1
pyrus_communis pycom11g19530
rosa_chinensis RchiOBHm_Chr5g0038891
rosa_laevigata RLG00000033879
rosa_multiflora Rmu_sc0000533.1_g000083
rosa_roxburghii Rroxscaffold_1G00042130
rosa_rugosa Rorug05G0174900
rosa_samantha Rh5BG266400 Rh5CG299400 Rh5DG273300
rosa_wichuraiana Rw5G024440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 32, 135, 257
AcoI YGGCCR 1 cut(s) 36
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 2 cut(s) 183, 432
AjnI CCWGG 3 cut(s) 83, 219, 294
AluBI AGCT 4 cut(s) 28, 179, 315, 351
AluI AGCT 4 cut(s) 28, 179, 315, 351
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 1 cut(s) 254
Ama87I CYCGRG 2 cut(s) 269, 338
AoxI GGCC 1 cut(s) 36
ApeKI GCWGC 1 cut(s) 312
AspLEI GCGC 1 cut(s) 10
AspS9I GGNCC 1 cut(s) 143
AsuHPI GGTGA 2 cut(s) 104, 368
AvaI CYCGRG 2 cut(s) 269, 338
AvaII GGWCC 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 327
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 1 cut(s) 324
BccI CCATC 2 cut(s) 146, 388
BceAI ACGGC 2 cut(s) 23, 118
BcgI CGANNNNNNTGC 2 cut(s) 319, 353
BciT130I CCWGG 3 cut(s) 85, 221, 296
BcoDI GTCTC 1 cut(s) 254
BfaI CTAG 1 cut(s) 161
BisI GCNGC 1 cut(s) 313
BlsI GCNGC 1 cut(s) 314
Bme1390I CCNGG 3 cut(s) 85, 221, 296
Bme18I GGWCC 1 cut(s) 143
BmeT110I CYCGRG 2 cut(s) 269, 338
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 2 cut(s) 82, 145
BmrFI CCNGG 3 cut(s) 85, 221, 296
BmsI GCATC 1 cut(s) 277
Bpu10I CCTNAGC 2 cut(s) 171, 352
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 2 cut(s) 39, 100
Bse118I RCCGGY 1 cut(s) 433
Bse1I ACTGG 1 cut(s) 94
BseBI CCWGG 3 cut(s) 85, 221, 296
BseDI CCNNGG 2 cut(s) 39, 100
BseGI GGATG 2 cut(s) 292, 380
BseMII CTCAG 2 cut(s) 162, 366
BseNI ACTGG 1 cut(s) 94
BseXI GCAGC 1 cut(s) 324
BsgI GTGCAG 1 cut(s) 313
BshFI GGCC 1 cut(s) 38
BsiHKAI GWGCWC 1 cut(s) 327
BsiHKCI CYCGRG 2 cut(s) 269, 338
BsiSI CCGG 3 cut(s) 72, 384, 434
BsmAI GTCTC 1 cut(s) 254
BsmI GAATGC 1 cut(s) 224
BsnI GGCC 1 cut(s) 38
BsoBI CYCGRG 2 cut(s) 269, 338
Bsp1286I GDGCHC 1 cut(s) 327
Bsp143I GATC 2 cut(s) 66, 151
BspACI CCGC 3 cut(s) 32, 135, 257
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 2 cut(s) 163, 365
BspLI GGNNCC 2 cut(s) 82, 145
BsrFI RCCGGY 1 cut(s) 433
BsrI ACTGG 1 cut(s) 94
BssAI RCCGGY 1 cut(s) 433
BssECI CCNNGG 2 cut(s) 39, 100
BssMI GATC 2 cut(s) 66, 151
BssNI GRCGYC 1 cut(s) 75
Bst2UI CCWGG 3 cut(s) 85, 221, 296
Bst4CI ACNGT 5 cut(s) 61, 186, 253, 374, 400
Bst6I CTCTTC 1 cut(s) 59
BstACI GRCGYC 1 cut(s) 75
BstC8I GCNNGC 2 cut(s) 30, 292
BstDEI CTNAG 2 cut(s) 171, 352
BstDSI CCRYGG 2 cut(s) 39, 100
BstF5I GGATG 2 cut(s) 292, 380
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 2 cut(s) 69, 154
BstMAI GTCTC 1 cut(s) 254
BstMBI GATC 2 cut(s) 66, 151
BstMWI GCNNNNNNNGC 2 cut(s) 176, 296
BstNI CCWGG 3 cut(s) 85, 221, 296
BstNSI RCATGY 1 cut(s) 294
BstSCI CCNGG 3 cut(s) 83, 219, 294
BstV1I GCAGC 1 cut(s) 324
BsuRI GGCC 1 cut(s) 38
BtgI CCRYGG 2 cut(s) 39, 100
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 2 cut(s) 292, 380
BtsI GCAGTG 1 cut(s) 325
BtsIMutI CAGTG 1 cut(s) 325
Cac8I GCNNGC 2 cut(s) 30, 292
CfoI GCGC 1 cut(s) 10
Cfr10I RCCGGY 1 cut(s) 433
Cfr13I GGNCC 1 cut(s) 143
CseI GACGC 1 cut(s) 64
Csp6I GTAC 2 cut(s) 182, 431
CviAII CATG 3 cut(s) 155, 291, 320
CviJI RGCY 7 cut(s) 28, 38, 83, 179, 315, 351, 387
CviKI_1 RGCY 7 cut(s) 28, 38, 83, 179, 315, 351, 387
CviQI GTAC 2 cut(s) 182, 431
DdeI CTNAG 2 cut(s) 171, 352
DpnI GATC 2 cut(s) 68, 153
DpnII GATC 2 cut(s) 66, 151
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
EciI GGCGGA 2 cut(s) 124, 272
Eco47I GGWCC 1 cut(s) 143
Eco88I CYCGRG 2 cut(s) 269, 338
EcoRII CCWGG 3 cut(s) 83, 219, 294
EcoT22I ATGCAT 1 cut(s) 292
FaeI CATG 3 cut(s) 158, 294, 323
FaiI YATR 6 cut(s) 156, 247, 249, 292, 321, 439
FatI CATG 3 cut(s) 154, 290, 319
Fnu4HI GCNGC 1 cut(s) 313
FokI GGATG 2 cut(s) 299, 367
Fsp4HI GCNGC 1 cut(s) 313
FspBI CTAG 1 cut(s) 161
GlaI GCGC 1 cut(s) 9
GluI GCNGC 1 cut(s) 313
HaeIII GGCC 1 cut(s) 38
HapII CCGG 3 cut(s) 72, 384, 434
HgaI GACGC 1 cut(s) 64
HhaI GCGC 1 cut(s) 10
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 3 cut(s) 158, 294, 323
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HinfI GANTC 1 cut(s) 131
HpaII CCGG 3 cut(s) 72, 384, 434
HphI GGTGA 2 cut(s) 104, 368
Hpy166II GTNNAC 1 cut(s) 366
Hpy188I TCNGA 2 cut(s) 80, 143
Hpy188III TCNNGA 3 cut(s) 64, 161, 229
Hpy8I GTNNAC 1 cut(s) 366
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 2 cut(s) 16, 158
HpyCH4III ACNGT 5 cut(s) 61, 186, 253, 374, 400
HpyCH4V TGCA 4 cut(s) 189, 290, 330, 444
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 296
HpyF3I CTNAG 2 cut(s) 171, 352
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 3 cut(s) 158, 294, 323
HspAI GCGC 1 cut(s) 8
Kzo9I GATC 2 cut(s) 66, 151
LmnI GCTCC 2 cut(s) 25, 80
Lsp1109I GCAGC 1 cut(s) 324
LweI GCATC 1 cut(s) 277
MaeI CTAG 1 cut(s) 161
MaeIII GTNAC 2 cut(s) 275, 374
MalI GATC 2 cut(s) 68, 153
MboI GATC 2 cut(s) 66, 151
MboII GAAGA 3 cut(s) 31, 46, 161
MfeI CAATTG 1 cut(s) 426
MhlI GDGCHC 1 cut(s) 327
MluCI AATT 2 cut(s) 214, 426
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 1 cut(s) 166
Mph1103I ATGCAT 1 cut(s) 292
MseI TTAA 1 cut(s) 402
MspI CCGG 3 cut(s) 72, 384, 434
MspR9I CCNGG 3 cut(s) 85, 221, 296
MunI CAATTG 1 cut(s) 426
Mva1269I GAATGC 1 cut(s) 224
MvaI CCWGG 3 cut(s) 85, 221, 296
MwoI GCNNNNNNNGC 2 cut(s) 176, 296
NdeII GATC 2 cut(s) 66, 151
NlaIII CATG 3 cut(s) 158, 294, 323
NlaIV GGNNCC 2 cut(s) 82, 145
NmuCI GTSAC 2 cut(s) 275, 374
NsiI ATGCAT 1 cut(s) 292
NspI RCATGY 1 cut(s) 294
PaeI GCATGC 1 cut(s) 294
PctI GAATGC 1 cut(s) 224
PkrI GCNGC 1 cut(s) 314
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
Psp6I CCWGG 3 cut(s) 83, 219, 294
PspGI CCWGG 3 cut(s) 83, 219, 294
PspN4I GGNNCC 2 cut(s) 82, 145
PspPI GGNCC 1 cut(s) 143
RsaI GTAC 2 cut(s) 183, 432
RsaNI GTAC 2 cut(s) 182, 431
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 1 cut(s) 313
Sau3AI GATC 2 cut(s) 66, 151
Sau96I GGNCC 1 cut(s) 143
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 3 cut(s) 85, 221, 296
SduI GDGCHC 1 cut(s) 327
SetI ASST 8 cut(s) 30, 169, 177, 181, 284, 307, 317, 353
SfaNI GCATC 1 cut(s) 277
SinI GGWCC 1 cut(s) 143
SphI GCATGC 1 cut(s) 294
Sse9I AATT 2 cut(s) 214, 426
SsiI CCGC 3 cut(s) 32, 135, 257
SspMI CTAG 1 cut(s) 161
StyD4I CCNGG 3 cut(s) 83, 219, 294
TaaI ACNGT 5 cut(s) 61, 186, 253, 374, 400
TaqI TCGA 1 cut(s) 418
TasI AATT 2 cut(s) 214, 426
TatI WGTACW 1 cut(s) 181
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TscAI CASTG 1 cut(s) 332
TseFI GTSAC 2 cut(s) 275, 374
TseI GCWGC 1 cut(s) 312
Tsp45I GTSAC 2 cut(s) 275, 374
TspDTI ATGAA 1 cut(s) 32
TspRI CASTG 1 cut(s) 332
VpaK11BI GGWCC 1 cut(s) 143
XbaI TCTAGA 1 cut(s) 160
XceI RCATGY 1 cut(s) 294
XspI CTAG 1 cut(s) 161
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.