MD03G1210500.v1.1

Trafficking protein particle complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011907
Physical Location & Seq
Forward (+)
28953637 .. 28957950
4314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1210500.v1.1.491

Sequence Viewer

Length: 561 bp
ATGCCTCCAGTTGGCCCTCGATCTGGTGATGCAATCTTCGCCAACGTGGAACGTGTGAATGCGGAGTTGTTTACATTGACGTACGGTGCGATTGTGCGCCAGCTGCTTACGGATCTTGAGGAAGTTGAAGAGGTTAACAAGCAGCTTGATCAAATGGGCTATAATATTGGCATTCGATTAATTGATGAGTTTCTAGCAAAGTCTAATGTATCTAGATGTGTTGACTTCAAAGAGACAGCTGAAGTAATTGCAAAGGTTGGTTTCAAAATGTTCTTGGGGGTCACTGCATCTGTTACAAATTGGGATGCTGATGGAACATGTTGCAGTATTGTTCTGGAGGACAATCCTCTGGTAGACTTTGTTGAGCTTCCAGATACGTGCCAAGGTCTTTACTATTGCAATATCTTAAGTGGAGTTGTAAGAGGCGCCTTAGAAATGGTATCAATGAAGACTGAAATTAGTTGGATCCGTGATATGCTTCGAGGTGATGATGCATTCGAGTTGCAAGTTAAACTCCTGAAGCAAATTCCAGAAGAGTACCCGTACAAAGATGATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAPP

Protein Analysis

187

Amino Acids

20.89

Weight (kDa)

4.36

Isoelectric Point (pI)

27.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAPP PF04051 24 - 171 2.4e-42 Transport protein particle (TRAPP) component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011907)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54750 AT5G54750
fragaria_vesca FvH4_3g21920
malus_domestica MD03G1210500.v1.1 MD11G1225200.v1.1
prunus_persica Prupe.4G204700_v2.0.a1
pyrus_communis pycom03g15920 pycom11g19870
rosa_chinensis RchiOBHm_Chr5g0037731
rosa_laevigata RLG00000033805
rosa_multiflora Rmu_sc0006558.1_g000039
rosa_roxburghii Rroxscaffold_1G00043170
rosa_rugosa Rorug05G0166800 Rorug05G0166900
rosa_samantha Rh5AG256100 Rh5BG258300 Rh5CG291600 Rh5DG268000
rosa_wichuraiana Rw5G023740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 425
AccI GTMKAC 1 cut(s) 354
AciI CCGC 1 cut(s) 62
AclWI GGATC 3 cut(s) 120, 460, 473
AcsI RAATTY 1 cut(s) 525
AcuI CTGAAG 2 cut(s) 261, 539
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 3 cut(s) 83, 539, 545
AfiI CCNNNNNNNGG 2 cut(s) 11, 23
AflII CTTAAG 1 cut(s) 406
AflIII ACRYGT 2 cut(s) 52, 317
AgsI TTSAA 3 cut(s) 128, 229, 265
AluBI AGCT 4 cut(s) 103, 145, 239, 367
AluI AGCT 4 cut(s) 103, 145, 239, 367
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 3 cut(s) 120, 460, 473
AoxI GGCC 1 cut(s) 13
ApeKI GCWGC 2 cut(s) 103, 142
ApoI RAATTY 1 cut(s) 525
AseI ATTAAT 1 cut(s) 179
AspLEI GCGC 2 cut(s) 99, 428
AspS9I GGNCC 1 cut(s) 14
AsuHPI GGTGA 2 cut(s) 38, 497
BamHI GGATCC 1 cut(s) 465
BanI GGYRCC 1 cut(s) 425
BbsI GAAGAC 1 cut(s) 455
BbvI GCAGC 2 cut(s) 90, 154
BccI CCATC 1 cut(s) 305
BclI TGATCA 1 cut(s) 148
BcoDI GTCTC 1 cut(s) 227
BfaI CTAG 2 cut(s) 194, 213
BfoI RGCGCY 1 cut(s) 429
BfrI CTTAAG 1 cut(s) 406
BisI GCNGC 2 cut(s) 104, 143
BlsI GCNGC 2 cut(s) 105, 144
BmgT120I GGNCC 1 cut(s) 14
BmiI GGNNCC 2 cut(s) 427, 467
BmsI GCATC 4 cut(s) 19, 295, 296, 481
BpiI GAAGAC 1 cut(s) 455
BpmI CTGGAG 1 cut(s) 356
BpuEI CTTGAG 1 cut(s) 137
BsaAI YACGTR 1 cut(s) 378
BsaHI GRCGYC 1 cut(s) 426
BsaJI CCNNGG 1 cut(s) 382
Bsc4I CCNNNNNNNGG 2 cut(s) 11, 23
Bse1I ACTGG 1 cut(s) 8
BseDI CCNNGG 1 cut(s) 382
BseGI GGATG 1 cut(s) 310
BseLI CCNNNNNNNGG 2 cut(s) 11, 23
BseNI ACTGG 1 cut(s) 8
BseXI GCAGC 2 cut(s) 90, 154
BshFI GGCC 1 cut(s) 15
BshNI GGYRCC 1 cut(s) 425
BsiWI CGTACG 1 cut(s) 81
BslI CCNNNNNNNGG 2 cut(s) 11, 23
BsmAI GTCTC 1 cut(s) 227
BsmI GAATGC 3 cut(s) 64, 171, 494
BsnI GGCC 1 cut(s) 15
Bsp143I GATC 4 cut(s) 20, 112, 148, 465
BspACI CCGC 1 cut(s) 62
BspANI GGCC 1 cut(s) 15
BspLI GGNNCC 2 cut(s) 427, 467
BspPI GGATC 3 cut(s) 120, 460, 473
BspT107I GGYRCC 1 cut(s) 425
BspTI CTTAAG 1 cut(s) 406
BsrI ACTGG 1 cut(s) 8
BssECI CCNNGG 1 cut(s) 382
BssMI GATC 4 cut(s) 20, 112, 148, 465
BssNI GRCGYC 1 cut(s) 426
BssT1I CCWWGG 1 cut(s) 382
Bst4CI ACNGT 1 cut(s) 86
Bst6I CTCTTC 2 cut(s) 123, 528
BstACI GRCGYC 1 cut(s) 426
BstAFI CTTAAG 1 cut(s) 406
BstBAI YACGTR 1 cut(s) 378
BstC8I GCNNGC 1 cut(s) 101
BstDEI CTNAG 1 cut(s) 430
BstF5I GGATG 1 cut(s) 310
BstH2I RGCGCY 1 cut(s) 429
BstHHI GCGC 2 cut(s) 99, 428
BstKTI GATC 4 cut(s) 23, 115, 151, 468
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 4 cut(s) 20, 112, 148, 465
BstMWI GCNNNNNNNGC 2 cut(s) 38, 103
BstNSI RCATGY 1 cut(s) 321
BstV1I GCAGC 2 cut(s) 90, 154
BstV2I GAAGAC 1 cut(s) 455
BstX2I RGATCY 2 cut(s) 112, 465
BstYI RGATCY 2 cut(s) 112, 465
BsuRI GGCC 1 cut(s) 15
BtsCI GGATG 1 cut(s) 310
BtsI GCAGTG 1 cut(s) 282
BtsIMutI CAGTG 1 cut(s) 282
Cac8I GCNNGC 1 cut(s) 101
CfoI GCGC 2 cut(s) 99, 428
Cfr13I GGNCC 1 cut(s) 14
Csp6I GTAC 3 cut(s) 82, 538, 544
CviAII CATG 1 cut(s) 318
CviJI RGCY 6 cut(s) 15, 103, 145, 159, 239, 367
CviKI_1 RGCY 6 cut(s) 15, 103, 145, 159, 239, 367
CviQI GTAC 3 cut(s) 82, 538, 544
DdeI CTNAG 1 cut(s) 430
DinI GGCGCC 1 cut(s) 427
DpnI GATC 4 cut(s) 22, 114, 150, 467
DpnII GATC 4 cut(s) 20, 112, 148, 465
Eam1104I CTCTTC 2 cut(s) 123, 528
EarI CTCTTC 2 cut(s) 123, 528
Eco130I CCWWGG 1 cut(s) 382
Eco57I CTGAAG 2 cut(s) 261, 539
EcoT14I CCWWGG 1 cut(s) 382
EcoT22I ATGCAT 1 cut(s) 496
EgeI GGCGCC 1 cut(s) 427
EheI GGCGCC 1 cut(s) 427
ErhI CCWWGG 1 cut(s) 382
FaeI CATG 1 cut(s) 321
FaiI YATR 3 cut(s) 162, 319, 476
FatI CATG 1 cut(s) 317
FbaI TGATCA 1 cut(s) 148
FblI GTMKAC 1 cut(s) 354
Fnu4HI GCNGC 2 cut(s) 104, 143
FokI GGATG 1 cut(s) 317
Fsp4HI GCNGC 2 cut(s) 104, 143
FspBI CTAG 2 cut(s) 194, 213
GlaI GCGC 2 cut(s) 98, 427
GluI GCNGC 2 cut(s) 104, 143
GsuI CTGGAG 1 cut(s) 356
HaeII RGCGCY 1 cut(s) 429
HaeIII GGCC 1 cut(s) 15
HhaI GCGC 2 cut(s) 99, 428
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 1 cut(s) 321
Hin6I GCGC 2 cut(s) 97, 426
HinP1I GCGC 2 cut(s) 97, 426
HincII GTYRAC 2 cut(s) 136, 223
HindII GTYRAC 2 cut(s) 136, 223
HpaI GTTAAC 1 cut(s) 136
HphI GGTGA 2 cut(s) 38, 497
Hpy166II GTNNAC 4 cut(s) 72, 136, 223, 355
Hpy188III TCNNGA 6 cut(s) 116, 213, 335, 371, 517, 530
Hpy8I GTNNAC 4 cut(s) 72, 136, 223, 355
HpyCH4III ACNGT 1 cut(s) 86
HpyCH4IV ACGT 4 cut(s) 45, 52, 80, 377
HpyCH4V TGCA 7 cut(s) 32, 251, 287, 324, 399, 494, 505
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 103
HpyF3I CTNAG 1 cut(s) 430
HpySE526I ACGT 4 cut(s) 45, 52, 80, 377
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 1 cut(s) 321
HspAI GCGC 2 cut(s) 97, 426
KasI GGCGCC 1 cut(s) 425
Ksp22I TGATCA 1 cut(s) 148
KspAI GTTAAC 1 cut(s) 136
Kzo9I GATC 4 cut(s) 20, 112, 148, 465
LpnPI CCDG 8 cut(s) 9, 21, 113, 320, 335, 384, 530, 543
Lsp1109I GCAGC 2 cut(s) 90, 154
LweI GCATC 4 cut(s) 19, 295, 296, 481
MaeI CTAG 2 cut(s) 194, 213
MaeII ACGT 4 cut(s) 45, 52, 80, 377
MaeIII GTNAC 2 cut(s) 280, 292
MalI GATC 4 cut(s) 22, 114, 150, 467
MboI GATC 4 cut(s) 20, 112, 148, 465
MboII GAAGA 4 cut(s) 28, 140, 460, 545
MflI RGATCY 2 cut(s) 112, 465
MluCI AATT 5 cut(s) 180, 246, 298, 456, 525
Mly113I GGCGCC 1 cut(s) 426
MmeI TCCRAC 1 cut(s) 443
MnlI CCTC 8 cut(s) 15, 27, 112, 124, 331, 357, 416, 476
Mph1103I ATGCAT 1 cut(s) 496
MseI TTAA 4 cut(s) 135, 179, 407, 510
MspA1I CMGCKG 2 cut(s) 103, 239
MspCI CTTAAG 1 cut(s) 406
Mva1269I GAATGC 3 cut(s) 64, 171, 494
MwoI GCNNNNNNNGC 2 cut(s) 38, 103
NarI GGCGCC 1 cut(s) 426
NdeII GATC 4 cut(s) 20, 112, 148, 465
NlaIII CATG 1 cut(s) 321
NlaIV GGNNCC 2 cut(s) 427, 467
NmuCI GTSAC 1 cut(s) 280
NsiI ATGCAT 1 cut(s) 496
NspI RCATGY 1 cut(s) 321
PciI ACATGT 1 cut(s) 317
PcsI WCGNNNNNNNCGW 1 cut(s) 86
PctI GAATGC 3 cut(s) 64, 171, 494
Pfl23II CGTACG 1 cut(s) 81
PkrI GCNGC 2 cut(s) 105, 144
PluTI GGCGCC 1 cut(s) 429
Ppu21I YACGTR 1 cut(s) 378
PscI ACATGT 1 cut(s) 317
PshBI ATTAAT 1 cut(s) 179
PspLI CGTACG 1 cut(s) 81
PspN4I GGNNCC 2 cut(s) 427, 467
PspPI GGNCC 1 cut(s) 14
PsuI RGATCY 2 cut(s) 112, 465
PvuII CAGCTG 2 cut(s) 103, 239
RsaI GTAC 3 cut(s) 83, 539, 545
RsaNI GTAC 3 cut(s) 82, 538, 544
SaqAI TTAA 4 cut(s) 135, 179, 407, 510
SatI GCNGC 2 cut(s) 104, 143
Sau3AI GATC 4 cut(s) 20, 112, 148, 465
Sau96I GGNCC 1 cut(s) 14
SfaNI GCATC 4 cut(s) 19, 295, 296, 481
SfoI GGCGCC 1 cut(s) 427
SmlI CTYRAG 2 cut(s) 116, 406
SmoI CTYRAG 2 cut(s) 116, 406
Sse9I AATT 5 cut(s) 180, 246, 298, 456, 525
SsiI CCGC 1 cut(s) 62
SspDI GGCGCC 1 cut(s) 425
SspI AATATT 1 cut(s) 166
SspMI CTAG 2 cut(s) 194, 213
StyI CCWWGG 1 cut(s) 382
TaaI ACNGT 1 cut(s) 86
TaiI ACGT 4 cut(s) 48, 55, 83, 380
TaqI TCGA 4 cut(s) 19, 175, 481, 498
TasI AATT 5 cut(s) 180, 246, 298, 456, 525
Tru1I TTAA 4 cut(s) 135, 179, 407, 510
Tru9I TTAA 4 cut(s) 135, 179, 407, 510
TscAI CASTG 1 cut(s) 289
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 2 cut(s) 103, 142
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 1 cut(s) 461
TspGWI ACGGA 2 cut(s) 125, 458
TspRI CASTG 1 cut(s) 289
Vha464I CTTAAG 1 cut(s) 406
VspI ATTAAT 1 cut(s) 179
XapI RAATTY 1 cut(s) 525
XbaI TCTAGA 1 cut(s) 212
XceI RCATGY 1 cut(s) 321
XmiI GTMKAC 1 cut(s) 354
XspI CTAG 2 cut(s) 194, 213
Zsp2I ATGCAT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.