MD03G1212500.v1.1

Domain of unknown function (DUF1995)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015382
Physical Location & Seq
Reverse (-)
29175397 .. 29175756
360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1212500.v1.1.491

Sequence Viewer

Length: 360 bp
ATGAAGCTCAAGAAGCCCAAACAACCCCGATTCCGACTTGAAATCCCTCTCGCCGATGACTCCCCGGAATCCCTCTCCCACCTCGCCCTCCAGCTCTTCCAAGACCTACCCATCAAACGAATATCCTCCAAAGTCAACGTCTTGAACGTATGGTCAAACGCCGCCTTCGCAGAAACCGCATTGAAAGCCTTTGGGGCTTGCCCTTCCACCCCGGTTGAGCATTCAGACATTTCCTCCATCGCCAACGATAATACCGGAAATTTGAATTCTGCTGACGTGGCGGTGTTTTTGGCGCCAGAGGGAACCCAACTGGCGGTTACAAAAAGGGACACTAATTTGTTGTTCCGTGGCCGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1995

Protein Analysis

120

Amino Acids

13.07

Weight (kDa)

8.89

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20680
fragaria_vesca FvH4_3g21740
malus_domestica MD03G1212500.v1.1 MD11G1227700.v1.1
prunus_persica Prupe.4G201600_v2.0.a1
pyrus_communis pycom03g16120
rosa_chinensis RchiOBHm_Chr5g0037211
rosa_laevigata RLG00000033765
rosa_multiflora Rmu_co8240257.1_g000001 Rmu_sc0006633.1_g000004
rosa_roxburghii Rroxscaffold_1G00043760
rosa_rugosa Rorug05G0163800
rosa_samantha Rh5BG255400 Rh5CG288100 Rh5DG263700
rosa_wichuraiana Rw5G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 292
AciI CCGC 4 cut(s) 162, 177, 281, 314
AcoI YGGCCR 1 cut(s) 349
AcsI RAATTY 2 cut(s) 259, 265
AcyI GRCGYC 1 cut(s) 293
AfiI CCNNNNNNNGG 1 cut(s) 313
AgsI TTSAA 4 cut(s) 41, 145, 184, 265
AjiI CACGTC 1 cut(s) 277
AluBI AGCT 2 cut(s) 7, 94
AluI AGCT 2 cut(s) 7, 94
AoxI GGCC 1 cut(s) 349
ApoI RAATTY 2 cut(s) 259, 265
ArsI GACNNNNNNTTYG 2 cut(s) 123, 155
AspLEI GCGC 1 cut(s) 295
AsuC2I CCSGG 2 cut(s) 65, 212
BanI GGYRCC 1 cut(s) 292
BccI CCATC 2 cut(s) 119, 245
BcnI CCSGG 2 cut(s) 65, 212
BfoI RGCGCY 1 cut(s) 296
BglI GCCNNNNNGGC 1 cut(s) 194
BisI GCNGC 1 cut(s) 162
BlsI GCNGC 1 cut(s) 163
Bme1390I CCNGG 2 cut(s) 65, 212
BmgBI CACGTC 1 cut(s) 277
BmiI GGNNCC 2 cut(s) 294, 304
BmrFI CCNGG 2 cut(s) 65, 212
BpmI CTGGAG 1 cut(s) 74
BpuMI CCSGG 2 cut(s) 65, 212
BsaHI GRCGYC 1 cut(s) 293
BsaJI CCNNGG 3 cut(s) 63, 210, 346
BsaWI WCCGGW 1 cut(s) 254
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 1 cut(s) 313
Bse118I RCCGGY 1 cut(s) 351
Bse1I ACTGG 1 cut(s) 315
BseDI CCNNGG 3 cut(s) 63, 210, 346
BseLI CCNNNNNNNGG 1 cut(s) 313
BseNI ACTGG 1 cut(s) 315
BshFI GGCC 1 cut(s) 351
BshNI GGYRCC 1 cut(s) 292
BsiSI CCGG 4 cut(s) 65, 212, 255, 352
BslFI GGGAC 1 cut(s) 341
BslI CCNNNNNNNGG 1 cut(s) 313
BsmFI GGGAC 1 cut(s) 341
BsmI GAATGC 1 cut(s) 220
BsnI GGCC 1 cut(s) 351
BspACI CCGC 4 cut(s) 162, 177, 281, 314
BspANI GGCC 1 cut(s) 351
BspLI GGNNCC 2 cut(s) 294, 304
BspQI GCTCTTC 1 cut(s) 101
BspT107I GGYRCC 1 cut(s) 292
BsrFI RCCGGY 1 cut(s) 351
BsrI ACTGG 1 cut(s) 315
BssAI RCCGGY 1 cut(s) 351
BssECI CCNNGG 3 cut(s) 63, 210, 346
BssNI GRCGYC 1 cut(s) 293
Bst6I CTCTTC 1 cut(s) 101
BstACI GRCGYC 1 cut(s) 293
BstC8I GCNNGC 1 cut(s) 199
BstDSI CCRYGG 1 cut(s) 346
BstH2I RGCGCY 1 cut(s) 296
BstHHI GCGC 1 cut(s) 295
BstMWI GCNNNNNNNGC 6 cut(s) 13, 167, 176, 185, 194, 278
BstSCI CCNGG 2 cut(s) 63, 210
BsuRI GGCC 1 cut(s) 351
BtgI CCRYGG 1 cut(s) 346
BtgZI GCGATG 1 cut(s) 223
BtrI CACGTC 1 cut(s) 277
Cac8I GCNNGC 1 cut(s) 199
CfoI GCGC 1 cut(s) 295
Cfr10I RCCGGY 1 cut(s) 351
CspCI CAANNNNNGTGG 2 cut(s) 196, 231
CviJI RGCY 6 cut(s) 7, 16, 94, 188, 197, 351
CviKI_1 RGCY 6 cut(s) 7, 16, 94, 188, 197, 351
DinI GGCGCC 1 cut(s) 294
EaeI YGGCCR 1 cut(s) 349
Eam1104I CTCTTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 101
EcoRI GAATTC 1 cut(s) 265
EgeI GGCGCC 1 cut(s) 294
EheI GGCGCC 1 cut(s) 294
FaiI YATR 1 cut(s) 151
FaqI GGGAC 1 cut(s) 341
Fnu4HI GCNGC 1 cut(s) 162
Fsp4HI GCNGC 1 cut(s) 162
GlaI GCGC 1 cut(s) 294
GluI GCNGC 1 cut(s) 162
GsuI CTGGAG 1 cut(s) 74
HaeII RGCGCY 1 cut(s) 296
HaeIII GGCC 1 cut(s) 351
HapII CCGG 4 cut(s) 65, 212, 255, 352
HhaI GCGC 1 cut(s) 295
Hin1I GRCGYC 1 cut(s) 293
Hin6I GCGC 1 cut(s) 293
HinP1I GCGC 1 cut(s) 293
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 3 cut(s) 30, 59, 68
HpaII CCGG 4 cut(s) 65, 212, 255, 352
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 2 cut(s) 35, 226
Hpy188III TCNNGA 2 cut(s) 10, 142
Hpy8I GTNNAC 1 cut(s) 136
HpyAV CCTTC 2 cut(s) 175, 213
HpyCH4IV ACGT 3 cut(s) 138, 147, 276
HpyF10VI GCNNNNNNNGC 6 cut(s) 13, 167, 176, 185, 194, 278
HpySE526I ACGT 3 cut(s) 138, 147, 276
Hsp92I GRCGYC 1 cut(s) 293
HspAI GCGC 1 cut(s) 293
KasI GGCGCC 1 cut(s) 292
LguI GCTCTTC 1 cut(s) 101
LpnPI CCDG 6 cut(s) 78, 104, 225, 268, 296, 309
MaeII ACGT 3 cut(s) 138, 147, 276
MaeIII GTNAC 1 cut(s) 316
MboII GAAGA 1 cut(s) 88
MluCI AATT 3 cut(s) 259, 265, 334
Mly113I GGCGCC 1 cut(s) 293
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 7 cut(s) 57, 83, 92, 98, 136, 244, 292
MspI CCGG 4 cut(s) 65, 212, 255, 352
MspR9I CCNGG 2 cut(s) 65, 212
Mva1269I GAATGC 1 cut(s) 220
MwoI GCNNNNNNNGC 6 cut(s) 13, 167, 176, 185, 194, 278
NarI GGCGCC 1 cut(s) 293
NciI CCSGG 2 cut(s) 65, 212
NlaIV GGNNCC 2 cut(s) 294, 304
PciSI GCTCTTC 1 cut(s) 101
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PctI GAATGC 1 cut(s) 220
PfeI GAWTC 2 cut(s) 30, 68
PkrI GCNGC 1 cut(s) 163
PleI GAGTC 1 cut(s) 53
PluTI GGCGCC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 53
PspN4I GGNNCC 2 cut(s) 294, 304
SapI GCTCTTC 1 cut(s) 101
SatI GCNGC 1 cut(s) 162
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 2 cut(s) 65, 212
SetI ASST 7 cut(s) 9, 84, 96, 108, 141, 150, 279
SfoI GGCGCC 1 cut(s) 294
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
Sse9I AATT 3 cut(s) 259, 265, 334
SsiI CCGC 4 cut(s) 162, 177, 281, 314
SspDI GGCGCC 1 cut(s) 292
StyD4I CCNGG 2 cut(s) 63, 210
TaiI ACGT 3 cut(s) 141, 150, 279
TasI AATT 3 cut(s) 259, 265, 334
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 2 cut(s) 30, 68
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 335
XapI RAATTY 2 cut(s) 259, 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.