Rw5G023400

Domain of unknown function (DUF1995)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0015382
Physical Location & Seq
Forward (+)
32925816 .. 32926828
1013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G023400.1

Sequence Viewer

Length: 981 bp
ATGGCTTCCAATCTTCTCAGAATTCATACAAAAACCATCAGAACACCAAACCCAGCAACCCCATTTTCACTCACCAAAACCCTCTCCTCCCCCTCTCTTCAATTTTCCACACACCACCACTCCCAACTCTCATTCCCTTCAACTCTCCTCAGCAAATCCAAAACTCCCTCTCTATCCCCACTACTCTGCTCCCTCTCCTCCCCCACCCCACCAAACTCAAAAGAAGCAGCCATCCAACAAGCCAAAACATGCCTCTCAACCACCTTAGAGAAGCCCCTCAACAACCTCAGACTCACCAGCAAGCTCAAGAAGCTCAAACAGCCCCGATTCCGTATCGAAATCCCGGTCGCCGACGACTCCCCCGAGTCCCTATCCCAACTCGCCCTCCAATTCTTCCGAGACCTCCCCATCAAAAGAAAAGGCTCCCCAGTCAACACCGTAATCCTATGGCCCGATATCACCTCCACAGAATCGGCACTTAAGGCCTTCAAGTCCTCCCCCTCAAGCACAGTTGAGCATTTAGACATTCCCTCAGTCGCCAACGGTAATACCAGAATTTCAAACAACGCTGACGTGGCGGTGTTTTTGGCGCCAGAACCATCCCAAGTGGCGGTTATGAAAACCGTTGTGGATTTGTTGTACCCGAGGCCGGTGGTTATTTTCAACCCCAAGTGGGGGTTTGAGGAAGAGGCCGAGTTTGGTGAGCTGAGTGGATTTGTGGGTTCGTTTGAGGTCATTTTTTCTAAAAGAAATGGGGTGGTTTTCAAGTGTGTGAGAAATGGGGTTGTGAGCGGTGAGAAGTGGGCGGTTCTTGTTGAAGAAGAACAAGGGGAATTGAAGGTGGTTTCGACGTTTAAGTCGAGGCCTTCGATTGCTGAAGTTGAGTATGTGTTGTATAATTTGATGGCCATGAATTCGCCTGTAACGAAATCCGTGAAGTTCTTTAAGGATTTGGTGTCAAATGTAACCGGAAAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1995

Protein Analysis

326

Amino Acids

35.87

Weight (kDa)

9.53

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1995 PF09353 71 - 295 3e-33 Domain of unknown function (DUF1995)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20680
fragaria_vesca FvH4_3g21740
malus_domestica MD03G1212500.v1.1 MD11G1227700.v1.1
prunus_persica Prupe.4G201600_v2.0.a1
pyrus_communis pycom03g16120
rosa_chinensis RchiOBHm_Chr5g0037211
rosa_laevigata RLG00000033765
rosa_multiflora Rmu_co8240257.1_g000001 Rmu_sc0006633.1_g000004
rosa_roxburghii Rroxscaffold_1G00043760
rosa_rugosa Rorug05G0163800
rosa_samantha Rh5BG255400 Rh5CG288100 Rh5DG263700
rosa_wichuraiana Rw5G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 856
AccB1I GGYRCC 1 cut(s) 589
AccBSI CCGCTC 1 cut(s) 792
AciI CCGC 4 cut(s) 578, 611, 792, 806
AcoI YGGCCR 1 cut(s) 906
AcsI RAATTY 3 cut(s) 21, 555, 913
AcuI CTGAAG 1 cut(s) 897
AcyI GRCGYC 1 cut(s) 590
AfaI GTAC 1 cut(s) 641
AfiI CCNNNNNNNGG 5 cut(s) 610, 649, 673, 674, 675
AflII CTTAAG 1 cut(s) 479
AgsI TTSAA 8 cut(s) 101, 141, 490, 561, 664, 766, 818, 838
AjiI CACGTC 1 cut(s) 574
AluBI AGCT 3 cut(s) 304, 313, 706
AluI AGCT 3 cut(s) 304, 313, 706
Alw26I GTCTC 1 cut(s) 393
Ama87I CYCGRG 2 cut(s) 362, 643
AoxI GGCC 6 cut(s) 449, 483, 647, 690, 863, 906
ApeKI GCWGC 1 cut(s) 227
ApoI RAATTY 3 cut(s) 21, 555, 913
ArsI GACNNNNNNTTYG 2 cut(s) 330, 362
AspLEI GCGC 1 cut(s) 592
AspS9I GGNCC 1 cut(s) 450
AsuC2I CCSGG 1 cut(s) 344
AsuHPI GGTGA 5 cut(s) 64, 286, 451, 713, 806
AvaI CYCGRG 2 cut(s) 362, 643
BalI TGGCCA 1 cut(s) 908
BanI GGYRCC 1 cut(s) 589
BbvCI CCTCAGC 1 cut(s) 149
BbvI GCAGC 1 cut(s) 239
BccI CCATC 5 cut(s) 44, 239, 416, 607, 898
BcnI CCSGG 1 cut(s) 344
BcoDI GTCTC 1 cut(s) 393
BfoI RGCGCY 1 cut(s) 593
BfrI CTTAAG 1 cut(s) 479
BisI GCNGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 229
Bme1390I CCNGG 1 cut(s) 344
BmeT110I CYCGRG 2 cut(s) 362, 643
BmgBI CACGTC 1 cut(s) 574
BmgT120I GGNCC 1 cut(s) 450
BmiI GGNNCC 2 cut(s) 424, 591
BmrFI CCNGG 1 cut(s) 344
BmrI ACTGGG 1 cut(s) 422
BmuI ACTGGG 1 cut(s) 422
Bpu10I CCTNAGC 1 cut(s) 149
BpuEI CTTGAG 2 cut(s) 290, 487
BpuMI CCSGG 1 cut(s) 344
BsaHI GRCGYC 1 cut(s) 590
BsaI GGTCTC 1 cut(s) 393
BsaJI CCNNGG 1 cut(s) 644
BsaWI WCCGGW 1 cut(s) 968
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 369, 399
Bsc4I CCNNNNNNNGG 5 cut(s) 610, 649, 673, 674, 675
Bse118I RCCGGY 1 cut(s) 649
Bse1I ACTGG 1 cut(s) 428
BseDI CCNNGG 1 cut(s) 644
BseGI GGATG 2 cut(s) 231, 599
BseLI CCNNNNNNNGG 5 cut(s) 610, 649, 673, 674, 675
BseMII CTCAG 5 cut(s) 31, 163, 301, 546, 698
BseNI ACTGG 1 cut(s) 428
BseRI GAGGAG 3 cut(s) 76, 137, 187
BseXI GCAGC 1 cut(s) 239
BseYI CCCAGC 1 cut(s) 52
Bsh1285I CGRYCG 1 cut(s) 348
BshFI GGCC 6 cut(s) 451, 485, 649, 692, 865, 908
BshNI GGYRCC 1 cut(s) 589
BsiEI CGRYCG 1 cut(s) 348
BsiHKCI CYCGRG 2 cut(s) 362, 643
BsiSI CCGG 3 cut(s) 344, 650, 969
BslFI GGGAC 1 cut(s) 352
BslI CCNNNNNNNGG 5 cut(s) 610, 649, 673, 674, 675
BsmAI GTCTC 1 cut(s) 393
BsmFI GGGAC 1 cut(s) 352
BsnI GGCC 6 cut(s) 451, 485, 649, 692, 865, 908
Bso31I GGTCTC 1 cut(s) 393
BsoBI CYCGRG 2 cut(s) 362, 643
BspACI CCGC 4 cut(s) 578, 611, 792, 806
BspANI GGCC 6 cut(s) 451, 485, 649, 692, 865, 908
BspCNI CTCAG 5 cut(s) 30, 162, 300, 545, 699
BspLI GGNNCC 2 cut(s) 424, 591
BspT107I GGYRCC 1 cut(s) 589
BspTI CTTAAG 1 cut(s) 479
BspTNI GGTCTC 1 cut(s) 393
BsrBI CCGCTC 1 cut(s) 792
BsrFI RCCGGY 1 cut(s) 649
BsrI ACTGG 1 cut(s) 428
BssAI RCCGGY 1 cut(s) 649
BssECI CCNNGG 1 cut(s) 644
BssNI GRCGYC 1 cut(s) 590
Bst4CI ACNGT 4 cut(s) 439, 511, 545, 625
Bst6I CTCTTC 2 cut(s) 102, 681
BstACI GRCGYC 1 cut(s) 590
BstAFI CTTAAG 1 cut(s) 479
BstC8I GCNNGC 1 cut(s) 302
BstDEI CTNAG 6 cut(s) 17, 149, 265, 287, 532, 707
BstF5I GGATG 2 cut(s) 231, 599
BstH2I RGCGCY 1 cut(s) 593
BstHHI GCGC 1 cut(s) 592
BstMAI GTCTC 1 cut(s) 393
BstMCI CGRYCG 1 cut(s) 348
BstMWI GCNNNNNNNGC 4 cut(s) 310, 319, 482, 575
BstNSI RCATGY 1 cut(s) 252
BstSCI CCNGG 1 cut(s) 342
BstV1I GCAGC 1 cut(s) 239
BsuRI GGCC 6 cut(s) 451, 485, 649, 692, 865, 908
BtrI CACGTC 1 cut(s) 574
BtsCI GGATG 2 cut(s) 231, 599
Cac8I GCNNGC 1 cut(s) 302
CfoI GCGC 1 cut(s) 592
Cfr10I RCCGGY 1 cut(s) 649
Cfr13I GGNCC 1 cut(s) 450
Csp6I GTAC 1 cut(s) 640
CviAII CATG 2 cut(s) 249, 910
CviQI GTAC 1 cut(s) 640
DdeI CTNAG 6 cut(s) 17, 149, 265, 287, 532, 707
DinI GGCGCC 1 cut(s) 591
DrdI GACNNNNNNGTC 1 cut(s) 856
DseDI GACNNNNNNGTC 1 cut(s) 856
EaeI YGGCCR 1 cut(s) 906
Eam1104I CTCTTC 2 cut(s) 102, 681
EarI CTCTTC 2 cut(s) 102, 681
Eco147I AGGCCT 2 cut(s) 485, 865
Eco31I GGTCTC 1 cut(s) 393
Eco32I GATATC 1 cut(s) 457
Eco57I CTGAAG 1 cut(s) 897
Eco88I CYCGRG 2 cut(s) 362, 643
EcoRI GAATTC 2 cut(s) 21, 913
EcoRV GATATC 1 cut(s) 457
EgeI GGCGCC 1 cut(s) 591
EheI GGCGCC 1 cut(s) 591
FaeI CATG 2 cut(s) 252, 913
FaiI YATR 7 cut(s) 27, 250, 448, 617, 888, 897, 911
FaqI GGGAC 1 cut(s) 352
FatI CATG 2 cut(s) 248, 909
Fnu4HI GCNGC 1 cut(s) 228
FokI GGATG 2 cut(s) 218, 586
Fsp4HI GCNGC 1 cut(s) 228
GlaI GCGC 1 cut(s) 591
GluI GCNGC 1 cut(s) 228
GsaI CCCAGC 1 cut(s) 56
HaeII RGCGCY 1 cut(s) 593
HaeIII GGCC 6 cut(s) 451, 485, 649, 692, 865, 908
HapII CCGG 3 cut(s) 344, 650, 969
HhaI GCGC 1 cut(s) 592
Hin1I GRCGYC 1 cut(s) 590
Hin1II CATG 2 cut(s) 252, 913
Hin6I GCGC 1 cut(s) 590
HinP1I GCGC 1 cut(s) 590
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HinfI GANTC 5 cut(s) 291, 327, 356, 365, 470
HpaII CCGG 3 cut(s) 344, 650, 969
HphI GGTGA 5 cut(s) 64, 286, 451, 713, 806
Hpy166II GTNNAC 1 cut(s) 433
Hpy188I TCNGA 4 cut(s) 20, 41, 290, 398
Hpy188III TCNNGA 1 cut(s) 307
Hpy8I GTNNAC 1 cut(s) 433
Hpy99I CGWCG 2 cut(s) 356, 853
HpyAV CCTTC 4 cut(s) 147, 496, 832, 876
HpyCH4III ACNGT 4 cut(s) 439, 511, 545, 625
HpyCH4IV ACGT 2 cut(s) 573, 851
HpyF10VI GCNNNNNNNGC 4 cut(s) 310, 319, 482, 575
HpyF3I CTNAG 6 cut(s) 17, 149, 265, 287, 532, 707
HpySE526I ACGT 2 cut(s) 573, 851
Hsp92I GRCGYC 1 cut(s) 590
Hsp92II CATG 2 cut(s) 252, 913
HspAI GCGC 1 cut(s) 590
KasI GGCGCC 1 cut(s) 589
LmnI GCTCC 2 cut(s) 194, 428
LpnPI CCDG 8 cut(s) 66, 310, 357, 441, 565, 606, 663, 933
Lsp1109I GCAGC 1 cut(s) 239
MaeII ACGT 2 cut(s) 573, 851
MaeIII GTNAC 2 cut(s) 922, 964
MbiI CCGCTC 1 cut(s) 792
MboII GAAGA 6 cut(s) 5, 89, 385, 698, 830, 833
MlsI TGGCCA 1 cut(s) 908
MluCI AATT 7 cut(s) 21, 101, 389, 555, 833, 898, 913
MluNI TGGCCA 1 cut(s) 908
Mly113I GGCGCC 1 cut(s) 590
MlyI GAGTC 3 cut(s) 285, 350, 374
MmeI TCCRAC 1 cut(s) 259
Mox20I TGGCCA 1 cut(s) 908
MscI TGGCCA 1 cut(s) 908
MseI TTAA 3 cut(s) 480, 855, 945
Msp20I TGGCCA 1 cut(s) 908
MspCI CTTAAG 1 cut(s) 479
MspI CCGG 3 cut(s) 344, 650, 969
MspR9I CCNGG 1 cut(s) 344
MwoI GCNNNNNNNGC 4 cut(s) 310, 319, 482, 575
NarI GGCGCC 1 cut(s) 590
NciI CCSGG 1 cut(s) 344
NlaIII CATG 2 cut(s) 252, 913
NlaIV GGNNCC 2 cut(s) 424, 591
NmeAIII GCCGAG 1 cut(s) 718
NspI RCATGY 1 cut(s) 252
PceI AGGCCT 2 cut(s) 485, 865
PcsI WCGNNNNNNNCGW 4 cut(s) 360, 857, 866, 923
PfeI GAWTC 2 cut(s) 327, 470
PkrI GCNGC 1 cut(s) 229
PleI GAGTC 3 cut(s) 285, 350, 373
PluTI GGCGCC 1 cut(s) 593
PpsI GAGTC 3 cut(s) 285, 350, 373
PspFI CCCAGC 1 cut(s) 52
PspN4I GGNNCC 2 cut(s) 424, 591
PspPI GGNCC 1 cut(s) 450
RsaI GTAC 1 cut(s) 641
RsaNI GTAC 1 cut(s) 640
SaqAI TTAA 3 cut(s) 480, 855, 945
SatI GCNGC 1 cut(s) 228
Sau96I GGNCC 1 cut(s) 450
SchI GAGTC 3 cut(s) 285, 350, 374
ScrFI CCNGG 1 cut(s) 344
SfoI GGCGCC 1 cut(s) 591
SmlI CTYRAG 3 cut(s) 305, 479, 502
SmoI CTYRAG 3 cut(s) 305, 479, 502
Sse9I AATT 7 cut(s) 21, 101, 389, 555, 833, 898, 913
SseBI AGGCCT 2 cut(s) 485, 865
SsiI CCGC 4 cut(s) 578, 611, 792, 806
SspDI GGCGCC 1 cut(s) 589
StuI AGGCCT 2 cut(s) 485, 865
StyD4I CCNGG 1 cut(s) 342
TaaI ACNGT 4 cut(s) 439, 511, 545, 625
TaiI ACGT 2 cut(s) 576, 854
TaqI TCGA 4 cut(s) 336, 848, 860, 869
TasI AATT 7 cut(s) 21, 101, 389, 555, 833, 898, 913
TfiI GAWTC 2 cut(s) 327, 470
Tru1I TTAA 3 cut(s) 480, 855, 945
Tru9I TTAA 3 cut(s) 480, 855, 945
TseI GCWGC 1 cut(s) 227
TspDTI ATGAA 3 cut(s) 14, 632, 926
TspGWI ACGGA 2 cut(s) 320, 922
Vha464I CTTAAG 1 cut(s) 479
XapI RAATTY 3 cut(s) 21, 555, 913
XceI RCATGY 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.