Prupe.4G201600_v2.0.a1

Domain of unknown function (DUF1995)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0015382
Physical Location & Seq
Forward (+)
12525328 .. 12526609
1282 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G201600.1

Sequence Viewer

Length: 1011 bp
ATGGCTTCCAATCTTCTCAGGGTTCATACAAAAACCATTAAAACTGCAAAGCAACCAAACCCATTTCCACTCACTAAAACTCTCTCTTTCACTTCTCTTCCATTTCCCACACACCAACACCACCAACATATCTCCTTGCCTCCAATTTTCCTCCACAAATCCAAAATTACTACTTTGTCCCCACTCTGCTCCTTATCTTCTCCCACCCCTCCAAGCTCAAAAGAAGCAGCCATTCAACAAGCCAAAACCTGTCTCTCAACCACATTGGAAAAGCCCCTCAACAACCTCAGACTCACCAACAAGCTCAAGAAGCTCAAGCAGCCCAGATTTCGTATCGAAATCCCGGTCATCGATGACTCTCCTGAATCCCTATCCCAGCTCGCTTTCCAGGTATTTCGAGACCTACCCATTAAAAGAAAAGGGTCCAAAGTTAACATCTTGATCATATGGCCTAACCTTGCCTACACAGAAGCTGCATTAAAAGCCTTCGAGTCTAACCCTTCAAGCCCAGTTGAAAATTTAGACATATATTCGATCACCAACGGTAATACCAGAATTTCGAGCTCTGCTGACGTGGCAGTGTTTGTGGCGCCAGAGGGAACCCAACTGGCTGATATGAAAAGTGTTGCTGATTTATTGTACCCAAGGCCAGTGGTTATTTTCAACCCCAAGTGGGTGTTTGAGGATGAAGCTGAGTTTGGTGAGCTGAGTGGCTTTGTGGGTTCATTTGAGGTCATTTTTTCATTTATGGGTTTGGAAGTTCAGGGGCTTTTGAGCAAAAGAAATGGAGTGATTTTCAAGTGTGTGAGAGATGGGGTTGTGAGCGGTGAGAAGTGGGCTGTTCTTGTTGAAGAGGAAGGGCAATTGAAAGCGGTTTCAACGTTTAAATCGCGGCCATCAATCAGTGAAGTTGAGAATGTTTTGTATAATTTGATGGCAATTAATTCGCCTGTTACGAAGTCCGTGAAGTTCTTCAGGGATTTGGTGTCTAATGTAACCGGAAAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1995

Protein Analysis

337

Amino Acids

37.27

Weight (kDa)

9.43

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20680
fragaria_vesca FvH4_3g21740
malus_domestica MD03G1212500.v1.1 MD11G1227700.v1.1
prunus_persica Prupe.4G201600_v2.0.a1
pyrus_communis pycom03g16120
rosa_chinensis RchiOBHm_Chr5g0037211
rosa_laevigata RLG00000033765
rosa_multiflora Rmu_co8240257.1_g000001 Rmu_sc0006633.1_g000004
rosa_roxburghii Rroxscaffold_1G00043760
rosa_rugosa Rorug05G0163800
rosa_samantha Rh5BG255400 Rh5CG288100 Rh5DG263700
rosa_wichuraiana Rw5G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 589
AccBSI CCGCTC 1 cut(s) 825
AccII CGCG 1 cut(s) 892
AciI CCGC 3 cut(s) 825, 872, 892
AclI AACGTT 1 cut(s) 881
AcoI YGGCCR 1 cut(s) 893
AcsI RAATTY 2 cut(s) 517, 555
AcuI CTGAAG 1 cut(s) 958
AcyI GRCGYC 1 cut(s) 590
AfaI GTAC 1 cut(s) 641
AfiI CCNNNNNNNGG 1 cut(s) 673
AgsI TTSAA 8 cut(s) 236, 504, 515, 664, 799, 851, 868, 879
AjiI CACGTC 1 cut(s) 574
AjnI CCWGG 1 cut(s) 387
AjuI GAANNNNNNNTTGG 2 cut(s) 681, 713
AluBI AGCT 8 cut(s) 216, 304, 313, 379, 473, 564, 692, 706
AluI AGCT 8 cut(s) 216, 304, 313, 379, 473, 564, 692, 706
Alw21I GWGCWC 1 cut(s) 566
Alw26I GTCTC 2 cut(s) 257, 393
AlwNI CAGNNNCTG 1 cut(s) 473
AoxI GGCC 3 cut(s) 449, 647, 893
ApeKI GCWGC 3 cut(s) 227, 319, 473
ApoI RAATTY 2 cut(s) 517, 555
ArsI GACNNNNNNTTYG 2 cut(s) 330, 362
AseI ATTAAT 1 cut(s) 942
Asp700I GAANNNNTTC 1 cut(s) 971
AspLEI GCGC 1 cut(s) 592
AspS9I GGNCC 1 cut(s) 423
AsuC2I CCSGG 1 cut(s) 344
AsuHPI GGTGA 4 cut(s) 286, 529, 713, 839
AvaII GGWCC 1 cut(s) 423
BanI GGYRCC 1 cut(s) 589
BanII GRGCYC 1 cut(s) 566
Bbv12I GWGCWC 1 cut(s) 566
BbvI GCAGC 3 cut(s) 239, 331, 460
BccI CCATC 3 cut(s) 806, 904, 928
BcgI CGANNNNNNTGC 2 cut(s) 927, 961
BciT130I CCWGG 1 cut(s) 389
BclI TGATCA 1 cut(s) 441
BcnI CCSGG 1 cut(s) 344
BcoDI GTCTC 2 cut(s) 257, 393
BfoI RGCGCY 1 cut(s) 593
BisI GCNGC 4 cut(s) 228, 320, 474, 893
BlsI GCNGC 4 cut(s) 229, 321, 475, 894
Bme1390I CCNGG 2 cut(s) 344, 389
Bme18I GGWCC 1 cut(s) 423
BmgBI CACGTC 1 cut(s) 574
BmgT120I GGNCC 1 cut(s) 423
BmiI GGNNCC 3 cut(s) 424, 591, 601
BmrFI CCNGG 2 cut(s) 344, 389
BmrI ACTGGG 1 cut(s) 503
BmuI ACTGGG 1 cut(s) 503
BpuEI CTTGAG 2 cut(s) 290, 299
BpuMI CCSGG 1 cut(s) 344
Bsa29I ATCGAT 1 cut(s) 351
BsaHI GRCGYC 1 cut(s) 590
BsaI GGTCTC 1 cut(s) 393
BsaJI CCNNGG 1 cut(s) 644
BsaWI WCCGGW 1 cut(s) 998
Bsc4I CCNNNNNNNGG 1 cut(s) 673
Bse1I ACTGG 3 cut(s) 509, 612, 650
BseBI CCWGG 1 cut(s) 389
BseCI ATCGAT 1 cut(s) 351
BseDI CCNNGG 1 cut(s) 644
BseGI GGATG 1 cut(s) 691
BseLI CCNNNNNNNGG 1 cut(s) 673
BseMII CTCAG 4 cut(s) 31, 301, 684, 698
BseNI ACTGG 3 cut(s) 509, 612, 650
BseXI GCAGC 3 cut(s) 239, 331, 460
BseYI CCCAGC 1 cut(s) 375
Bsh1236I CGCG 1 cut(s) 892
BshFI GGCC 3 cut(s) 451, 649, 895
BshNI GGYRCC 1 cut(s) 589
BshVI ATCGAT 1 cut(s) 351
BsiHKAI GWGCWC 1 cut(s) 566
BsiSI CCGG 2 cut(s) 344, 999
BslFI GGGAC 1 cut(s) 163
BslI CCNNNNNNNGG 1 cut(s) 673
BsmAI GTCTC 2 cut(s) 257, 393
BsmFI GGGAC 1 cut(s) 163
BsnI GGCC 3 cut(s) 451, 649, 895
Bso31I GGTCTC 1 cut(s) 393
Bsp1286I GDGCHC 1 cut(s) 566
Bsp143I GATC 2 cut(s) 441, 534
BspACI CCGC 3 cut(s) 825, 872, 892
BspANI GGCC 3 cut(s) 451, 649, 895
BspCNI CTCAG 4 cut(s) 30, 300, 685, 699
BspDI ATCGAT 1 cut(s) 351
BspFNI CGCG 1 cut(s) 892
BspLI GGNNCC 3 cut(s) 424, 591, 601
BspT107I GGYRCC 1 cut(s) 589
BspTNI GGTCTC 1 cut(s) 393
BsrBI CCGCTC 1 cut(s) 825
BsrI ACTGG 3 cut(s) 509, 612, 650
BssECI CCNNGG 1 cut(s) 644
BssMI GATC 2 cut(s) 441, 534
BssNI GRCGYC 1 cut(s) 590
BssT1I CCWWGG 1 cut(s) 644
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 1 cut(s) 545
Bst6I CTCTTC 2 cut(s) 102, 846
BstACI GRCGYC 1 cut(s) 590
BstC8I GCNNGC 1 cut(s) 381
BstDEI CTNAG 4 cut(s) 17, 287, 693, 707
BstF5I GGATG 1 cut(s) 691
BstFNI CGCG 1 cut(s) 892
BstH2I RGCGCY 1 cut(s) 593
BstHHI GCGC 1 cut(s) 592
BstKTI GATC 2 cut(s) 444, 537
BstMAI GTCTC 2 cut(s) 257, 393
BstMBI GATC 2 cut(s) 441, 534
BstMWI GCNNNNNNNGC 4 cut(s) 310, 319, 482, 575
BstNI CCWGG 1 cut(s) 389
BstSCI CCNGG 2 cut(s) 342, 387
BstUI CGCG 1 cut(s) 892
BstV1I GCAGC 3 cut(s) 239, 331, 460
Bsu15I ATCGAT 1 cut(s) 351
BsuRI GGCC 3 cut(s) 451, 649, 895
BsuTUI ATCGAT 1 cut(s) 351
BtrI CACGTC 1 cut(s) 574
BtsCI GGATG 1 cut(s) 691
BtsI GCAGTG 1 cut(s) 585
BtsIMutI CAGTG 3 cut(s) 585, 657, 910
Cac8I GCNNGC 1 cut(s) 381
CaiI CAGNNNCTG 1 cut(s) 473
CfoI GCGC 1 cut(s) 592
Cfr13I GGNCC 1 cut(s) 423
ClaI ATCGAT 1 cut(s) 351
Csp6I GTAC 1 cut(s) 640
CspCI CAANNNNNGTGG 2 cut(s) 633, 668
CviQI GTAC 1 cut(s) 640
DdeI CTNAG 4 cut(s) 17, 287, 693, 707
DinI GGCGCC 1 cut(s) 591
DpnI GATC 2 cut(s) 443, 536
DpnII GATC 2 cut(s) 441, 534
DraI TTTAAA 1 cut(s) 886
EaeI YGGCCR 1 cut(s) 893
Eam1104I CTCTTC 2 cut(s) 102, 846
EarI CTCTTC 2 cut(s) 102, 846
Ecl136II GAGCTC 1 cut(s) 564
Eco130I CCWWGG 1 cut(s) 644
Eco24I GRGCYC 1 cut(s) 566
Eco31I GGTCTC 1 cut(s) 393
Eco47I GGWCC 1 cut(s) 423
Eco53kI GAGCTC 1 cut(s) 564
Eco57I CTGAAG 1 cut(s) 958
EcoICRI GAGCTC 1 cut(s) 564
EcoRII CCWGG 1 cut(s) 387
EcoT14I CCWWGG 1 cut(s) 644
EcoT38I GRGCYC 1 cut(s) 566
EgeI GGCGCC 1 cut(s) 591
EheI GGCGCC 1 cut(s) 591
ErhI CCWWGG 1 cut(s) 644
FaiI YATR 9 cut(s) 27, 129, 446, 448, 527, 529, 617, 749, 927
FaqI GGGAC 1 cut(s) 163
FauNDI CATATG 1 cut(s) 446
FbaI TGATCA 1 cut(s) 441
Fnu4HI GCNGC 4 cut(s) 228, 320, 474, 893
FokI GGATG 1 cut(s) 698
FriOI GRGCYC 1 cut(s) 566
Fsp4HI GCNGC 4 cut(s) 228, 320, 474, 893
GlaI GCGC 1 cut(s) 591
GluI GCNGC 4 cut(s) 228, 320, 474, 893
GsaI CCCAGC 1 cut(s) 379
HaeII RGCGCY 1 cut(s) 593
HaeIII GGCC 3 cut(s) 451, 649, 895
HapII CCGG 2 cut(s) 344, 999
HhaI GCGC 1 cut(s) 592
Hin1I GRCGYC 1 cut(s) 590
Hin6I GCGC 1 cut(s) 590
HinP1I GCGC 1 cut(s) 590
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HinfI GANTC 4 cut(s) 291, 356, 365, 491
HpaI GTTAAC 1 cut(s) 433
HpaII CCGG 2 cut(s) 344, 999
HphI GGTGA 4 cut(s) 286, 529, 713, 839
Hpy166II GTNNAC 1 cut(s) 433
Hpy188I TCNGA 1 cut(s) 290
Hpy188III TCNNGA 4 cut(s) 307, 362, 398, 439
Hpy8I GTNNAC 1 cut(s) 433
HpyAV CCTTC 3 cut(s) 496, 510, 851
HpyCH4III ACNGT 1 cut(s) 545
HpyCH4IV ACGT 2 cut(s) 573, 881
HpyCH4V TGCA 2 cut(s) 47, 476
HpyF10VI GCNNNNNNNGC 4 cut(s) 310, 319, 482, 575
HpyF3I CTNAG 4 cut(s) 17, 287, 693, 707
HpySE526I ACGT 2 cut(s) 573, 881
Hsp92I GRCGYC 1 cut(s) 590
HspAI GCGC 1 cut(s) 590
KasI GGCGCC 1 cut(s) 589
Ksp22I TGATCA 1 cut(s) 441
KspAI GTTAAC 1 cut(s) 433
Kzo9I GATC 2 cut(s) 441, 534
LmnI GCTCC 1 cut(s) 194
Lsp1109I GCAGC 3 cut(s) 239, 331, 460
MaeII ACGT 2 cut(s) 573, 881
MaeIII GTNAC 2 cut(s) 952, 994
MalI GATC 2 cut(s) 443, 536
MbiI CCGCTC 1 cut(s) 825
MboI GATC 2 cut(s) 441, 534
MboII GAAGA 5 cut(s) 5, 89, 189, 863, 964
MfeI CAATTG 1 cut(s) 863
MhlI GDGCHC 1 cut(s) 566
MluCI AATT 8 cut(s) 144, 165, 517, 555, 863, 928, 939, 943
Mly113I GGCGCC 1 cut(s) 590
MlyI GAGTC 3 cut(s) 285, 350, 500
MnlI CCTC 9 cut(s) 150, 161, 219, 287, 296, 589, 676, 724, 847
MroXI GAANNNNTTC 1 cut(s) 971
MseI TTAA 6 cut(s) 39, 411, 432, 479, 885, 942
MspI CCGG 2 cut(s) 344, 999
MspR9I CCNGG 2 cut(s) 344, 389
MunI CAATTG 1 cut(s) 863
MvaI CCWGG 1 cut(s) 389
MvnI CGCG 1 cut(s) 892
MwoI GCNNNNNNNGC 4 cut(s) 310, 319, 482, 575
NarI GGCGCC 1 cut(s) 590
NciI CCSGG 1 cut(s) 344
NdeI CATATG 1 cut(s) 446
NdeII GATC 2 cut(s) 441, 534
NlaIV GGNNCC 3 cut(s) 424, 591, 601
PcsI WCGNNNNNNNCGW 1 cut(s) 953
PdmI GAANNNNTTC 1 cut(s) 971
PfeI GAWTC 1 cut(s) 365
PkrI GCNGC 4 cut(s) 229, 321, 475, 894
PleI GAGTC 3 cut(s) 285, 350, 499
PluTI GGCGCC 1 cut(s) 593
PpsI GAGTC 3 cut(s) 285, 350, 499
PshBI ATTAAT 1 cut(s) 942
Psp124BI GAGCTC 1 cut(s) 566
Psp1406I AACGTT 1 cut(s) 881
Psp6I CCWGG 1 cut(s) 387
PspFI CCCAGC 1 cut(s) 375
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 3 cut(s) 424, 591, 601
PspPI GGNCC 1 cut(s) 423
PstNI CAGNNNCTG 1 cut(s) 473
RsaI GTAC 1 cut(s) 641
RsaNI GTAC 1 cut(s) 640
SacI GAGCTC 1 cut(s) 566
SaqAI TTAA 6 cut(s) 39, 411, 432, 479, 885, 942
SatI GCNGC 4 cut(s) 228, 320, 474, 893
Sau3AI GATC 2 cut(s) 441, 534
Sau96I GGNCC 1 cut(s) 423
SchI GAGTC 3 cut(s) 285, 350, 500
ScrFI CCNGG 2 cut(s) 344, 389
SduI GDGCHC 1 cut(s) 566
SfoI GGCGCC 1 cut(s) 591
SinI GGWCC 1 cut(s) 423
SmlI CTYRAG 2 cut(s) 305, 314
SmoI CTYRAG 2 cut(s) 305, 314
Sse9I AATT 8 cut(s) 144, 165, 517, 555, 863, 928, 939, 943
SsiI CCGC 3 cut(s) 825, 872, 892
SspDI GGCGCC 1 cut(s) 589
SstI GAGCTC 1 cut(s) 566
StyD4I CCNGG 2 cut(s) 342, 387
StyI CCWWGG 1 cut(s) 644
TaaI ACNGT 1 cut(s) 545
TaiI ACGT 2 cut(s) 576, 884
TaqI TCGA 6 cut(s) 336, 351, 397, 489, 533, 560
TasI AATT 8 cut(s) 144, 165, 517, 555, 863, 928, 939, 943
TauI GCSGC 1 cut(s) 895
TfiI GAWTC 1 cut(s) 365
Tru1I TTAA 6 cut(s) 39, 411, 432, 479, 885, 942
Tru9I TTAA 6 cut(s) 39, 411, 432, 479, 885, 942
TscAI CASTG 3 cut(s) 585, 657, 910
TseI GCWGC 3 cut(s) 227, 319, 473
TspDTI ATGAA 5 cut(s) 14, 632, 702, 714, 732
TspGWI ACGGA 1 cut(s) 952
TspRI CASTG 3 cut(s) 585, 657, 910
VpaK11BI GGWCC 1 cut(s) 423
VspI ATTAAT 1 cut(s) 942
XapI RAATTY 2 cut(s) 517, 555
XmnI GAANNNNTTC 1 cut(s) 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.