MD11G1227700.v1.1

Domain of unknown function (DUF1995)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0015382
Physical Location & Seq
Reverse (-)
33140156 .. 33141148
993 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1227700.v1.1.491

Sequence Viewer

Length: 993 bp
ATGGCTACCAACCTTCTCAAAATCCACACGCAAACCATCAAAACCCCAAACCCGCCACCACCAACCCACCCATTCCCATTTCCCAAACACCACCAAAACTCTCACATCTCCTTCCCCTCAACCCTCCACCACAAACCCAAAATTACCCCCCTATCCCCACTCTGCTCCTCCTTATCCTCCTCCCCCACCCCACCAACCTCCAAAGAAGCCGCCATCCAACAGGCCAAAACCTGCCTCTCCGCCACCCTCTCCAAGCCCCTCAACAACCTCCGCCTCACCACCAAGCTCAAGAAGCCCAAACAACCCCGATTCCGACTCGAAATCCCCGTCGCCGACGACTCCCCGGAATCCCTCTCCAACCTCGCCATCCAGCTCTTCCAAGACCTTCCCATCAAACGAAGATCCTCCAAGGTCAACGTCTTGATTATATGGTCAAACGCTGCCTTCGCAGAATCCGCAGTGAAAGCCTTCGGGGCTTCCCCATGGAGCCCGGTTGAGCATTCGGACATTTCCTCCATCGCCAATGGTAATACCGATAATTTGAATTCTGCTGACGTGGTGGTGTTTTTGGCGCCAGAGGGAACCCAACTGGCGGTTATAAAAAGGGTTACTGATTTGCTGTTCCCGAGGCCGGTGGTGATTTTCAACCCCAGGTGGGCGTTCGAGGAGGAGGCGGAGTTGGGCGAGCTCGGCTGGTTCGTGGATTCGTTTGAGGTGATTTTTTCGTTCATGGGTTTGGAGGTGAAGGGGCTTTTGAGCAGCAGGAGTGGAGTGGTTTTCAGGTGCGTGAGAGATGGGGTTGTGAGTGGTGAGAAATGGGCTGTTTTTGTTGAGGAAGGAGGGGAATTGAAGATGGTTTCGACGTTTAAATCGCGGCCTTCAATCGGTGAAGTTGAGAGCGTTTTGTATAATGTGATGGCCATTAATTCACCCGTGACGAAATCAGTGAAGTTTTTCAGGGATTTGGTGTCGAATGTGACCGGAAAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1995

Protein Analysis

331

Amino Acids

36.29

Weight (kDa)

9.38

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1995 PF09353 65 - 299 1.8e-33 Domain of unknown function (DUF1995)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20680
fragaria_vesca FvH4_3g21740
malus_domestica MD03G1212500.v1.1 MD11G1227700.v1.1
prunus_persica Prupe.4G201600_v2.0.a1
pyrus_communis pycom03g16120
rosa_chinensis RchiOBHm_Chr5g0037211
rosa_laevigata RLG00000033765
rosa_multiflora Rmu_co8240257.1_g000001 Rmu_sc0006633.1_g000004
rosa_roxburghii Rroxscaffold_1G00043760
rosa_rugosa Rorug05G0163800
rosa_samantha Rh5BG255400 Rh5CG288100 Rh5DG263700
rosa_wichuraiana Rw5G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 599
Acc36I ACCTGC 1 cut(s) 239
AccB1I GGYRCC 1 cut(s) 571
AccII CGCG 1 cut(s) 874
AciI CCGC 8 cut(s) 53, 210, 240, 271, 456, 593, 674, 874
AclWI GGATC 1 cut(s) 396
AcoI YGGCCR 1 cut(s) 918
AcsI RAATTY 1 cut(s) 544
AcyI GRCGYC 1 cut(s) 572
AfiI CCNNNNNNNGG 4 cut(s) 592, 631, 655, 884
AgsI TTSAA 4 cut(s) 544, 646, 850, 882
AjiI CACGTC 1 cut(s) 556
AjnI CCWGG 1 cut(s) 650
AluBI AGCT 3 cut(s) 286, 373, 688
AluI AGCT 3 cut(s) 286, 373, 688
Alw21I GWGCWC 1 cut(s) 690
AlwI GGATC 1 cut(s) 396
Ama87I CYCGRG 1 cut(s) 625
AoxI GGCC 4 cut(s) 222, 629, 875, 918
ApeKI GCWGC 2 cut(s) 440, 759
ApoI RAATTY 1 cut(s) 544
ArsI GACNNNNNNTTYG 2 cut(s) 312, 344
AseI ATTAAT 1 cut(s) 924
Asp700I GAANNNNTTC 2 cut(s) 467, 953
AspLEI GCGC 1 cut(s) 574
AsuC2I CCSGG 2 cut(s) 344, 491
AsuHPI GGTGA 7 cut(s) 268, 649, 727, 754, 821, 899, 921
AvaI CYCGRG 1 cut(s) 625
BalI TGGCCA 1 cut(s) 920
BanI GGYRCC 1 cut(s) 571
BanII GRGCYC 2 cut(s) 491, 690
Bbv12I GWGCWC 1 cut(s) 690
BbvI GCAGC 2 cut(s) 427, 771
BccI CCATC 8 cut(s) 44, 221, 374, 398, 524, 788, 847, 910
BciT130I CCWGG 1 cut(s) 652
BcnI CCSGG 2 cut(s) 344, 491
BfoI RGCGCY 1 cut(s) 575
BfuAI ACCTGC 1 cut(s) 239
BglI GCCNNNNNGGC 1 cut(s) 473
BisI GCNGC 4 cut(s) 210, 441, 760, 875
BlsI GCNGC 4 cut(s) 211, 442, 761, 876
Bme1390I CCNGG 3 cut(s) 344, 491, 652
BmeT110I CYCGRG 1 cut(s) 625
BmgBI CACGTC 1 cut(s) 556
BmiI GGNNCC 3 cut(s) 488, 573, 583
BmrFI CCNGG 3 cut(s) 344, 491, 652
BpuEI CTTGAG 1 cut(s) 272
BpuMI CCSGG 2 cut(s) 344, 491
BsaHI GRCGYC 1 cut(s) 572
BsaJI CCNNGG 5 cut(s) 342, 408, 482, 626, 650
BsaWI WCCGGW 1 cut(s) 980
BsaXI ACNNNNNCTCC 4 cut(s) 497, 527, 662, 692
Bsc4I CCNNNNNNNGG 4 cut(s) 592, 631, 655, 884
Bse118I RCCGGY 1 cut(s) 631
Bse1I ACTGG 1 cut(s) 594
BseBI CCWGG 1 cut(s) 652
BseDI CCNNGG 5 cut(s) 342, 408, 482, 626, 650
BseGI GGATG 2 cut(s) 213, 366
BseLI CCNNNNNNNGG 4 cut(s) 592, 631, 655, 884
BseNI ACTGG 1 cut(s) 594
BseRI GAGGAG 4 cut(s) 157, 169, 680, 683
BseXI GCAGC 2 cut(s) 427, 771
Bsh1236I CGCG 1 cut(s) 874
BshFI GGCC 4 cut(s) 224, 631, 877, 920
BshNI GGYRCC 1 cut(s) 571
BsiHKAI GWGCWC 1 cut(s) 690
BsiHKCI CYCGRG 1 cut(s) 625
BsiSI CCGG 4 cut(s) 344, 491, 632, 981
BslI CCNNNNNNNGG 4 cut(s) 592, 631, 655, 884
BsmI GAATGC 1 cut(s) 499
BsnI GGCC 4 cut(s) 224, 631, 877, 920
BsoBI CYCGRG 1 cut(s) 625
Bsp1286I GDGCHC 2 cut(s) 491, 690
Bsp143I GATC 1 cut(s) 401
Bsp19I CCATGG 1 cut(s) 482
BspACI CCGC 8 cut(s) 53, 210, 240, 271, 456, 593, 674, 874
BspANI GGCC 4 cut(s) 224, 631, 877, 920
BspFNI CGCG 1 cut(s) 874
BspLI GGNNCC 3 cut(s) 488, 573, 583
BspMI ACCTGC 1 cut(s) 239
BspPI GGATC 1 cut(s) 396
BspQI GCTCTTC 1 cut(s) 380
BspT107I GGYRCC 1 cut(s) 571
BsrFI RCCGGY 1 cut(s) 631
BsrI ACTGG 1 cut(s) 594
BssAI RCCGGY 1 cut(s) 631
BssECI CCNNGG 5 cut(s) 342, 408, 482, 626, 650
BssMI GATC 1 cut(s) 401
BssNI GRCGYC 1 cut(s) 572
BssT1I CCWWGG 2 cut(s) 408, 482
Bst2UI CCWGG 1 cut(s) 652
Bst6I CTCTTC 1 cut(s) 380
BstACI GRCGYC 1 cut(s) 572
BstC8I GCNNGC 1 cut(s) 686
BstDSI CCRYGG 1 cut(s) 482
BstF5I GGATG 2 cut(s) 213, 366
BstFNI CGCG 1 cut(s) 874
BstH2I RGCGCY 1 cut(s) 575
BstHHI GCGC 1 cut(s) 574
BstKTI GATC 1 cut(s) 404
BstMBI GATC 1 cut(s) 401
BstMWI GCNNNNNNNGC 6 cut(s) 292, 446, 455, 464, 473, 690
BstNI CCWGG 1 cut(s) 652
BstSCI CCNGG 3 cut(s) 342, 489, 650
BstUI CGCG 1 cut(s) 874
BstV1I GCAGC 2 cut(s) 427, 771
BstX2I RGATCY 1 cut(s) 401
BstYI RGATCY 1 cut(s) 401
BsuRI GGCC 4 cut(s) 224, 631, 877, 920
BtgI CCRYGG 1 cut(s) 482
BtgZI GCGATG 1 cut(s) 502
BtrI CACGTC 1 cut(s) 556
BtsCI GGATG 2 cut(s) 213, 366
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 2 cut(s) 465, 951
BveI ACCTGC 1 cut(s) 239
Cac8I GCNNGC 1 cut(s) 686
CfoI GCGC 1 cut(s) 574
Cfr10I RCCGGY 1 cut(s) 631
CviAII CATG 2 cut(s) 483, 730
DinI GGCGCC 1 cut(s) 573
DpnI GATC 1 cut(s) 403
DpnII GATC 1 cut(s) 401
DraI TTTAAA 1 cut(s) 868
EaeI YGGCCR 1 cut(s) 918
Eam1104I CTCTTC 1 cut(s) 380
EarI CTCTTC 1 cut(s) 380
EciI GGCGGA 3 cut(s) 229, 260, 689
Ecl136II GAGCTC 1 cut(s) 688
Eco130I CCWWGG 2 cut(s) 408, 482
Eco24I GRGCYC 2 cut(s) 491, 690
Eco53kI GAGCTC 1 cut(s) 688
Eco88I CYCGRG 1 cut(s) 625
EcoICRI GAGCTC 1 cut(s) 688
EcoRI GAATTC 1 cut(s) 544
EcoRII CCWGG 1 cut(s) 650
EcoT14I CCWWGG 2 cut(s) 408, 482
EcoT38I GRGCYC 2 cut(s) 491, 690
EgeI GGCGCC 1 cut(s) 573
EheI GGCGCC 1 cut(s) 573
ErhI CCWWGG 2 cut(s) 408, 482
FaeI CATG 2 cut(s) 486, 733
FaiI YATR 6 cut(s) 428, 430, 484, 599, 731, 909
FatI CATG 2 cut(s) 482, 729
FauI CCCGC 1 cut(s) 60
Fnu4HI GCNGC 4 cut(s) 210, 441, 760, 875
FokI GGATG 2 cut(s) 200, 353
FriOI GRGCYC 2 cut(s) 491, 690
Fsp4HI GCNGC 4 cut(s) 210, 441, 760, 875
GlaI GCGC 1 cut(s) 573
GluI GCNGC 4 cut(s) 210, 441, 760, 875
HaeII RGCGCY 1 cut(s) 575
HaeIII GGCC 4 cut(s) 224, 631, 877, 920
HapII CCGG 4 cut(s) 344, 491, 632, 981
HhaI GCGC 1 cut(s) 574
Hin1I GRCGYC 1 cut(s) 572
Hin1II CATG 2 cut(s) 486, 733
Hin6I GCGC 1 cut(s) 572
HinP1I GCGC 1 cut(s) 572
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HinfI GANTC 6 cut(s) 309, 315, 338, 347, 452, 704
HpaII CCGG 4 cut(s) 344, 491, 632, 981
HphI GGTGA 7 cut(s) 268, 649, 727, 754, 821, 899, 921
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 2 cut(s) 314, 505
Hpy188III TCNNGA 3 cut(s) 289, 421, 625
Hpy8I GTNNAC 1 cut(s) 415
Hpy99I CGWCG 3 cut(s) 332, 338, 865
HpyAV CCTTC 8 cut(s) 23, 121, 395, 454, 478, 739, 830, 888
HpyCH4IV ACGT 3 cut(s) 417, 555, 863
HpyF10VI GCNNNNNNNGC 6 cut(s) 292, 446, 455, 464, 473, 690
HpySE526I ACGT 3 cut(s) 417, 555, 863
Hsp92I GRCGYC 1 cut(s) 572
Hsp92II CATG 2 cut(s) 486, 733
HspAI GCGC 1 cut(s) 572
KasI GGCGCC 1 cut(s) 571
Kzo9I GATC 1 cut(s) 401
LguI GCTCTTC 1 cut(s) 380
LmnI GCTCC 2 cut(s) 170, 486
Lsp1109I GCAGC 2 cut(s) 427, 771
MaeII ACGT 3 cut(s) 417, 555, 863
MaeIII GTNAC 3 cut(s) 607, 934, 976
MalI GATC 1 cut(s) 403
MboI GATC 1 cut(s) 401
MboII GAAGA 3 cut(s) 367, 411, 862
MflI RGATCY 1 cut(s) 401
MhlI GDGCHC 2 cut(s) 491, 690
MlsI TGGCCA 1 cut(s) 920
MluCI AATT 5 cut(s) 141, 538, 544, 845, 925
MluNI TGGCCA 1 cut(s) 920
Mly113I GGCGCC 1 cut(s) 572
MlyI GAGTC 2 cut(s) 309, 332
MmeI TCCRAC 3 cut(s) 241, 337, 381
Mox20I TGGCCA 1 cut(s) 920
MroXI GAANNNNTTC 2 cut(s) 467, 953
MscI TGGCCA 1 cut(s) 920
MseI TTAA 2 cut(s) 867, 924
Msp20I TGGCCA 1 cut(s) 920
MspI CCGG 4 cut(s) 344, 491, 632, 981
MspR9I CCNGG 3 cut(s) 344, 491, 652
Mva1269I GAATGC 1 cut(s) 499
MvaI CCWGG 1 cut(s) 652
MvnI CGCG 1 cut(s) 874
MwoI GCNNNNNNNGC 6 cut(s) 292, 446, 455, 464, 473, 690
NarI GGCGCC 1 cut(s) 572
NciI CCSGG 2 cut(s) 344, 491
NcoI CCATGG 1 cut(s) 482
NdeII GATC 1 cut(s) 401
NlaIII CATG 2 cut(s) 486, 733
NlaIV GGNNCC 3 cut(s) 488, 573, 583
NmeAIII GCCGAG 1 cut(s) 669
NmuCI GTSAC 2 cut(s) 934, 976
PciSI GCTCTTC 1 cut(s) 380
PcsI WCGNNNNNNNCGW 2 cut(s) 324, 696
PctI GAATGC 1 cut(s) 499
PdmI GAANNNNTTC 2 cut(s) 467, 953
PfeI GAWTC 4 cut(s) 309, 347, 452, 704
PkrI GCNGC 4 cut(s) 211, 442, 761, 876
PleI GAGTC 2 cut(s) 309, 332
PluTI GGCGCC 1 cut(s) 575
PpsI GAGTC 2 cut(s) 309, 332
PshBI ATTAAT 1 cut(s) 924
PsiI TTATAA 1 cut(s) 599
Psp124BI GAGCTC 1 cut(s) 690
Psp6I CCWGG 1 cut(s) 650
PspGI CCWGG 1 cut(s) 650
PspN4I GGNNCC 3 cut(s) 488, 573, 583
PsuI RGATCY 1 cut(s) 401
SacI GAGCTC 1 cut(s) 690
SapI GCTCTTC 1 cut(s) 380
SaqAI TTAA 2 cut(s) 867, 924
SatI GCNGC 4 cut(s) 210, 441, 760, 875
Sau3AI GATC 1 cut(s) 401
SchI GAGTC 2 cut(s) 309, 332
ScrFI CCNGG 3 cut(s) 344, 491, 652
SduI GDGCHC 2 cut(s) 491, 690
SfoI GGCGCC 1 cut(s) 573
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 5 cut(s) 141, 538, 544, 845, 925
SsiI CCGC 8 cut(s) 53, 210, 240, 271, 456, 593, 674, 874
SspDI GGCGCC 1 cut(s) 571
SstI GAGCTC 1 cut(s) 690
StyD4I CCNGG 3 cut(s) 342, 489, 650
StyI CCWWGG 2 cut(s) 408, 482
TaiI ACGT 3 cut(s) 420, 558, 866
TaqI TCGA 4 cut(s) 318, 663, 860, 971
TasI AATT 5 cut(s) 141, 538, 544, 845, 925
TauI GCSGC 2 cut(s) 212, 877
TfiI GAWTC 4 cut(s) 309, 347, 452, 704
Tru1I TTAA 2 cut(s) 867, 924
Tru9I TTAA 2 cut(s) 867, 924
TscAI CASTG 2 cut(s) 465, 951
TseFI GTSAC 2 cut(s) 934, 976
TseI GCWGC 2 cut(s) 440, 759
Tsp45I GTSAC 2 cut(s) 934, 976
TspDTI ATGAA 1 cut(s) 718
TspRI CASTG 2 cut(s) 465, 951
VspI ATTAAT 1 cut(s) 924
XapI RAATTY 1 cut(s) 544
XmnI GAANNNNTTC 2 cut(s) 467, 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.