MD03G1219700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0016788
Physical Location & Seq
Forward (+)
30425787 .. 30427723
1937 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1219700.v1.1.491

Sequence Viewer

Length: 447 bp
ATGGCCGCCCTTAGAACTGAGAGACCCTCCTCTAAGCTGGAAGCGCACAAATCCAACCCCAACCGCCGAGAAAAAATTGGCGGCGGGCTTCTGAGCAACATCGCCAAATTCGCCATCGACTCCACCATCAACTACTCTTTCAAGGCCTTCCCTGGTAGGAAGCAAGCGCATCAGATTGTGCGGGAAGGATTAAAGGACCATCCACCCCTTTCAGCATCACTTGACGACAAGAAGAAACCGGAAGATCTTAAGTTAGTGATGGAGGAAATGCACGCGAAAATGGAGCAGATGCAAGAAGACACGAATATTGTAAAGCAGCAACATAAGACATCAGCAGAGCATGTCGAAGGATCCGGGCATCCAAAGAAGATGGCGGATGAAGGTGTTCAGGGTTCGGATCTTCTGCAGAACAAGAAGAAAAAGATCTTCATTCGTTCGCGTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

16.69

Weight (kDa)

9.7

Isoelectric Point (pI)

53.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016788)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g20870
malus_domestica MD03G1219700.v1.1
prunus_persica Prupe.4G189200_v2.0.a1
pyrus_communis pycom03g16910
rosa_laevigata RLG00000033612
rosa_multiflora Rmu_sc0007948.1_g000002 Rmu_sc0008116.1_g000008
rosa_roxburghii Rroxscaffold_1G00045130
rosa_rugosa Rorug05G0150900
rosa_samantha Rh5BG243300 Rh5CG275000 Rh5DG252400 Rh5DG252500
rosa_wichuraiana Rw5G022270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 275, 439
AciI CCGC 6 cut(s) 6, 64, 81, 84, 181, 374
AclWI GGATC 3 cut(s) 345, 358, 405
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 107
AflII CTTAAG 1 cut(s) 248
AgsI TTSAA 1 cut(s) 142
AjnI CCWGG 1 cut(s) 151
AluBI AGCT 1 cut(s) 37
AluI AGCT 1 cut(s) 37
Alw26I GTCTC 1 cut(s) 16
AlwI GGATC 3 cut(s) 345, 358, 405
AoxI GGCC 2 cut(s) 3, 144
ApeKI GCWGC 1 cut(s) 316
ApoI RAATTY 1 cut(s) 107
Asp700I GAANNNNTTC 1 cut(s) 384
AspLEI GCGC 2 cut(s) 46, 169
AspS9I GGNCC 1 cut(s) 196
AsuC2I CCSGG 1 cut(s) 355
AvaII GGWCC 1 cut(s) 196
BamHI GGATCC 1 cut(s) 350
BbsI GAAGAC 1 cut(s) 303
BbvI GCAGC 1 cut(s) 328
BccI CCATC 5 cut(s) 122, 134, 207, 253, 364
BciT130I CCWGG 1 cut(s) 153
BcnI CCSGG 1 cut(s) 355
BcoDI GTCTC 1 cut(s) 16
BfmI CTRYAG 1 cut(s) 404
BfrI CTTAAG 1 cut(s) 248
BglII AGATCT 2 cut(s) 244, 423
BisI GCNGC 3 cut(s) 6, 82, 317
BlsI GCNGC 3 cut(s) 7, 83, 318
Bme1390I CCNGG 2 cut(s) 153, 355
Bme18I GGWCC 1 cut(s) 196
BmgT120I GGNCC 1 cut(s) 196
BmiI GGNNCC 1 cut(s) 352
BmrFI CCNGG 2 cut(s) 153, 355
BmsI GCATC 4 cut(s) 178, 224, 279, 367
BpiI GAAGAC 1 cut(s) 303
BplI GAGNNNNNCTC 2 cut(s) 11, 43
BpuMI CCSGG 1 cut(s) 355
BsaI GGTCTC 1 cut(s) 16
BsaJI CCNNGG 1 cut(s) 151
BsaWI WCCGGW 1 cut(s) 238
BseBI CCWGG 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 151
BseGI GGATG 3 cut(s) 199, 358, 382
BseMII CTCAG 2 cut(s) 9, 83
BseRI GAGGAG 1 cut(s) 19
BseXI GCAGC 1 cut(s) 328
Bsh1236I CGCG 2 cut(s) 275, 439
BshFI GGCC 2 cut(s) 5, 146
BsiSI CCGG 2 cut(s) 239, 354
BsmAI GTCTC 1 cut(s) 16
BsnI GGCC 2 cut(s) 5, 146
Bso31I GGTCTC 1 cut(s) 16
Bsp143I GATC 4 cut(s) 244, 350, 397, 423
BspACI CCGC 6 cut(s) 6, 64, 81, 84, 181, 374
BspANI GGCC 2 cut(s) 5, 146
BspCNI CTCAG 2 cut(s) 10, 84
BspFNI CGCG 2 cut(s) 275, 439
BspLI GGNNCC 1 cut(s) 352
BspMAI CTGCAG 1 cut(s) 408
BspPI GGATC 3 cut(s) 345, 358, 405
BspTI CTTAAG 1 cut(s) 248
BspTNI GGTCTC 1 cut(s) 16
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 4 cut(s) 244, 350, 397, 423
Bst2UI CCWGG 1 cut(s) 153
BstAFI CTTAAG 1 cut(s) 248
BstC8I GCNNGC 3 cut(s) 86, 165, 273
BstDEI CTNAG 4 cut(s) 11, 18, 33, 92
BstF5I GGATG 3 cut(s) 199, 358, 382
BstFNI CGCG 2 cut(s) 275, 439
BstHHI GCGC 2 cut(s) 46, 169
BstKTI GATC 4 cut(s) 247, 353, 400, 426
BstMAI GTCTC 1 cut(s) 16
BstMBI GATC 4 cut(s) 244, 350, 397, 423
BstMWI GCNNNNNNNGC 2 cut(s) 43, 110
BstNI CCWGG 1 cut(s) 153
BstNSI RCATGY 1 cut(s) 344
BstSCI CCNGG 2 cut(s) 151, 353
BstSFI CTRYAG 1 cut(s) 404
BstUI CGCG 2 cut(s) 275, 439
BstV1I GCAGC 1 cut(s) 328
BstV2I GAAGAC 1 cut(s) 303
BstX2I RGATCY 4 cut(s) 244, 350, 397, 423
BstYI RGATCY 4 cut(s) 244, 350, 397, 423
BsuRI GGCC 2 cut(s) 5, 146
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 3 cut(s) 199, 358, 382
Cac8I GCNNGC 3 cut(s) 86, 165, 273
CfoI GCGC 2 cut(s) 46, 169
Cfr13I GGNCC 1 cut(s) 196
CseI GACGC 1 cut(s) 428
CviAII CATG 1 cut(s) 341
CviJI RGCY 4 cut(s) 5, 37, 88, 146
CviKI_1 RGCY 4 cut(s) 5, 37, 88, 146
DdeI CTNAG 4 cut(s) 11, 18, 33, 92
DpnI GATC 4 cut(s) 246, 352, 399, 425
DpnII GATC 4 cut(s) 244, 350, 397, 423
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 389
Eco147I AGGCCT 1 cut(s) 146
Eco31I GGTCTC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 196
EcoRII CCWGG 1 cut(s) 151
FaeI CATG 1 cut(s) 344
FaiI YATR 2 cut(s) 324, 342
FatI CATG 1 cut(s) 340
FauI CCCGC 2 cut(s) 77, 174
Fnu4HI GCNGC 3 cut(s) 6, 82, 317
FokI GGATG 3 cut(s) 186, 345, 389
Fsp4HI GCNGC 3 cut(s) 6, 82, 317
GlaI GCGC 2 cut(s) 45, 168
GluI GCNGC 3 cut(s) 6, 82, 317
HaeIII GGCC 2 cut(s) 5, 146
HapII CCGG 2 cut(s) 239, 354
HgaI GACGC 1 cut(s) 428
HhaI GCGC 2 cut(s) 46, 169
Hin1II CATG 1 cut(s) 344
Hin6I GCGC 2 cut(s) 44, 167
HinP1I GCGC 2 cut(s) 44, 167
HinfI GANTC 1 cut(s) 119
HpaII CCGG 2 cut(s) 239, 354
Hpy188I TCNGA 3 cut(s) 93, 174, 397
HpyAV CCTTC 4 cut(s) 157, 179, 341, 374
HpyCH4V TGCA 3 cut(s) 271, 292, 406
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 110
HpyF3I CTNAG 4 cut(s) 11, 18, 33, 92
Hsp92II CATG 1 cut(s) 344
HspAI GCGC 2 cut(s) 44, 167
Kzo9I GATC 4 cut(s) 244, 350, 397, 423
LmnI GCTCC 1 cut(s) 283
LpnPI CCDG 6 cut(s) 23, 138, 165, 252, 367, 374
Lsp1109I GCAGC 1 cut(s) 328
LweI GCATC 4 cut(s) 178, 224, 279, 367
MalI GATC 4 cut(s) 246, 352, 399, 425
MboI GATC 4 cut(s) 244, 350, 397, 423
MboII GAAGA 7 cut(s) 244, 254, 308, 379, 392, 418, 427
MflI RGATCY 4 cut(s) 244, 350, 397, 423
MluCI AATT 2 cut(s) 75, 107
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 3 cut(s) 37, 40, 256
MroXI GAANNNNTTC 1 cut(s) 384
MseI TTAA 2 cut(s) 191, 249
MspCI CTTAAG 1 cut(s) 248
MspI CCGG 2 cut(s) 239, 354
MspR9I CCNGG 2 cut(s) 153, 355
MvaI CCWGG 1 cut(s) 153
MvnI CGCG 2 cut(s) 275, 439
MwoI GCNNNNNNNGC 2 cut(s) 43, 110
NciI CCSGG 1 cut(s) 355
NdeII GATC 4 cut(s) 244, 350, 397, 423
NlaIII CATG 1 cut(s) 344
NlaIV GGNNCC 1 cut(s) 352
NmeAIII GCCGAG 1 cut(s) 92
NspI RCATGY 1 cut(s) 344
PceI AGGCCT 1 cut(s) 146
PdmI GAANNNNTTC 1 cut(s) 384
PkrI GCNGC 3 cut(s) 7, 83, 318
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
Psp6I CCWGG 1 cut(s) 151
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 1 cut(s) 352
PspPI GGNCC 1 cut(s) 196
PstI CTGCAG 1 cut(s) 408
PsuI RGATCY 4 cut(s) 244, 350, 397, 423
SaqAI TTAA 2 cut(s) 191, 249
SatI GCNGC 3 cut(s) 6, 82, 317
Sau3AI GATC 4 cut(s) 244, 350, 397, 423
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 2 cut(s) 153, 355
SetI ASST 2 cut(s) 39, 385
SfaNI GCATC 4 cut(s) 178, 224, 279, 367
SfcI CTRYAG 1 cut(s) 404
SinI GGWCC 1 cut(s) 196
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 2 cut(s) 75, 107
SseBI AGGCCT 1 cut(s) 146
SsiI CCGC 6 cut(s) 6, 64, 81, 84, 181, 374
SspI AATATT 1 cut(s) 307
StuI AGGCCT 1 cut(s) 146
StyD4I CCNGG 2 cut(s) 151, 353
TaqI TCGA 2 cut(s) 117, 345
TasI AATT 2 cut(s) 75, 107
TauI GCSGC 2 cut(s) 8, 84
Tru1I TTAA 2 cut(s) 191, 249
Tru9I TTAA 2 cut(s) 191, 249
TseI GCWGC 1 cut(s) 316
TspDTI ATGAA 2 cut(s) 393, 418
Vha464I CTTAAG 1 cut(s) 248
VpaK11BI GGWCC 1 cut(s) 196
XapI RAATTY 1 cut(s) 107
XceI RCATGY 1 cut(s) 344
XmnI GAANNNNTTC 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.