MD03G1223300.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014079
Physical Location & Seq
Reverse (-)
30818821 .. 30821801
2981 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1223300.v1.1.491

Sequence Viewer

Length: 948 bp
ATGGTTATTGCTAAGCTGTTTGTAGTGTTTCTACTAATGCAAATGATTAGTGGCTTCCCATTTCGAGCAAATGGTTTGAGTATGGGCTATTACATTATGAGCTGCCCGATGGCTGAACTTATTGTCAGAAATACAGTCACCAGAGCCTTGCAAGCCGATCCAACTTTAGCAGCAGGTCTTATCAGAATGCACTTCCATGACTGCTTCATAGAGGGATGTGATGCGTCAATTCTCCTGGATTCAACAAAAGATAACAAGGCTGAGAAGGACTCCCCAGCAAATTTGAGCTTGCGTGGCTATGAGGTTATAGATGATGCAAAAGAGGAACTTGAAGAACAATGCCCTGGTGTTGTCTCTTGCGCCGACATAGTTGCAATGGCTTCTACTTATGCAGTTTATGCGTCTGGGGGTCCGGTATATGACATACCCAAAGGAAGAAAGGATGGAAGTAGGTCTAAAATAGAAGACACAAGAAATCTGCCTCCACCCACCTTGAATGCCTCTGAGCTCATCACAATGTTTGGGCAACATGGATTTACTGAGCAAGAAATGGTTGCTTTATCTGGTGCTCACACACTAGGAGTAGCCAGGTGCTCCTCATTCAAAAACCGATTGAGTGGTACTGTGGATCCAAATTTAGATTCAGGCTTTGCAAAGCAACTGGCCAAAACATGCAGCGCAGGCGATAACGCGGAGCAACCCTTCGATGCAACGAGAAACATTTTTGATAATGTCTACTTCAACGGACTGCAAAGGAAGGCTGGAGTCCTGAGTTCTGATCAAACCCTATTTGCCAGTGCGAGAACTAGAAACATTGTAAACGGTTATGCCTTCAACCAAGCTATGTTCTTCTTAGATTTTCAACAGGCGATGGTGAAAATGAGCACCCTCGATGTCAAGGAAGGTTCGAAAGGAGAAGTAAGAAAAGACTGCCGCAAAATCAACTAA

Protein Analysis

316

Amino Acids

34.46

Weight (kDa)

5.88

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 42 - 278 6.8e-84 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014079)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33420 AT4G33420
fragaria_vesca FvH4_3g20670 FvH4_3g20670
malus_domestica MD03G1223300.v1.1 MD11G1241100.v1.1
prunus_persica Prupe.4G186300_v2.0.a1
pyrus_communis pycom03g17160 pycom11g21270
rosa_chinensis RchiOBHm_Chr5g0034661
rosa_laevigata RLG00000033570
rosa_multiflora Rmu_sc0002460.1_g000077
rosa_roxburghii Rroxscaffold_1G00045630
rosa_rugosa Rorug05G0147700
rosa_samantha Rh5AG240100 Rh5DG248800
rosa_wichuraiana Rw5G021970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 164
AccI GTMKAC 1 cut(s) 735
AccII CGCG 1 cut(s) 692
AciI CCGC 2 cut(s) 692, 934
AclWI GGATC 3 cut(s) 152, 623, 636
AcoI YGGCCR 1 cut(s) 663
AcsI RAATTY 2 cut(s) 280, 634
AfaI GTAC 1 cut(s) 622
AgsI TTSAA 7 cut(s) 243, 332, 496, 604, 742, 835, 863
AjnI CCWGG 3 cut(s) 234, 343, 587
AjuI GAANNNNNNNTTGG 2 cut(s) 625, 657
AluBI AGCT 5 cut(s) 16, 102, 288, 508, 842
AluI AGCT 5 cut(s) 16, 102, 288, 508, 842
Alw21I GWGCWC 4 cut(s) 510, 571, 596, 887
Alw26I GTCTC 1 cut(s) 358
AlwI GGATC 3 cut(s) 152, 623, 636
AoxI GGCC 1 cut(s) 663
ApeKI GCWGC 3 cut(s) 102, 170, 675
ApoI RAATTY 2 cut(s) 280, 634
AspLEI GCGC 2 cut(s) 362, 680
AspS9I GGNCC 1 cut(s) 410
AsuHPI GGTGA 2 cut(s) 130, 886
AsuII TTCGAA 1 cut(s) 908
AvaII GGWCC 1 cut(s) 410
BalI TGGCCA 1 cut(s) 665
BamHI GGATCC 1 cut(s) 628
BanII GRGCYC 1 cut(s) 510
BbsI GAAGAC 1 cut(s) 471
Bbv12I GWGCWC 4 cut(s) 510, 571, 596, 887
BbvI GCAGC 3 cut(s) 89, 182, 687
BccI CCATC 3 cut(s) 103, 437, 865
BcgI CGANNNNNNTGC 2 cut(s) 353, 387
BciT130I CCWGG 3 cut(s) 236, 345, 589
BclI TGATCA 1 cut(s) 778
BcoDI GTCTC 1 cut(s) 358
BfaI CTAG 2 cut(s) 578, 807
BfuAI ACCTGC 1 cut(s) 164
BisI GCNGC 4 cut(s) 103, 171, 676, 934
BlpI GCTNAGC 1 cut(s) 12
BlsI GCNGC 4 cut(s) 104, 172, 677, 935
Bme1390I CCNGG 3 cut(s) 236, 345, 589
Bme18I GGWCC 1 cut(s) 410
BmgT120I GGNCC 1 cut(s) 410
BmiI GGNNCC 2 cut(s) 411, 630
BmrFI CCNGG 3 cut(s) 236, 345, 589
BmsI GCATC 3 cut(s) 211, 304, 697
BpiI GAAGAC 1 cut(s) 471
BplI GAGNNNNNCTC 2 cut(s) 254, 286
BpmI CTGGAG 1 cut(s) 783
Bpu1102I GCTNAGC 1 cut(s) 12
Bpu14I TTCGAA 1 cut(s) 908
BsaJI CCNNGG 1 cut(s) 343
BsaWI WCCGGW 1 cut(s) 412
Bse1I ACTGG 2 cut(s) 666, 795
Bse3DI GCAATG 1 cut(s) 381
BseBI CCWGG 3 cut(s) 236, 345, 589
BseDI CCNNGG 1 cut(s) 343
BseGI GGATG 2 cut(s) 221, 448
BseMI GCAATG 1 cut(s) 381
BseMII CTCAG 4 cut(s) 252, 495, 531, 761
BseNI ACTGG 2 cut(s) 666, 795
BseRI GAGGAG 1 cut(s) 586
BseXI GCAGC 3 cut(s) 89, 182, 687
BseYI CCCAGC 1 cut(s) 274
Bsh1236I CGCG 1 cut(s) 692
BshFI GGCC 1 cut(s) 665
BsiHKAI GWGCWC 4 cut(s) 510, 571, 596, 887
BsiSI CCGG 1 cut(s) 413
BsmAI GTCTC 1 cut(s) 358
BsmI GAATGC 2 cut(s) 192, 502
BsnI GGCC 1 cut(s) 665
Bsp119I TTCGAA 1 cut(s) 908
Bsp1286I GDGCHC 4 cut(s) 510, 571, 596, 887
Bsp143I GATC 3 cut(s) 157, 628, 778
Bsp1720I GCTNAGC 1 cut(s) 12
BspACI CCGC 2 cut(s) 692, 934
BspANI GGCC 1 cut(s) 665
BspCNI CTCAG 4 cut(s) 253, 496, 532, 762
BspFNI CGCG 1 cut(s) 692
BspLI GGNNCC 2 cut(s) 411, 630
BspMI ACCTGC 1 cut(s) 164
BspPI GGATC 3 cut(s) 152, 623, 636
BspT104I TTCGAA 1 cut(s) 908
BsrDI GCAATG 1 cut(s) 381
BsrI ACTGG 2 cut(s) 666, 795
BssECI CCNNGG 1 cut(s) 343
BssMI GATC 3 cut(s) 157, 628, 778
Bst2UI CCWGG 3 cut(s) 236, 345, 589
Bst4CI ACNGT 3 cut(s) 136, 625, 824
BstAPI GCANNNNNTGC 1 cut(s) 398
BstBI TTCGAA 1 cut(s) 908
BstC8I GCNNGC 3 cut(s) 153, 290, 682
BstDEI CTNAG 6 cut(s) 12, 261, 504, 540, 770, 853
BstF5I GGATG 2 cut(s) 221, 448
BstFNI CGCG 1 cut(s) 692
BstHHI GCGC 2 cut(s) 362, 680
BstKTI GATC 3 cut(s) 160, 631, 781
BstMAI GTCTC 1 cut(s) 358
BstMBI GATC 3 cut(s) 157, 628, 778
BstMWI GCNNNNNNNGC 4 cut(s) 152, 294, 398, 681
BstNI CCWGG 3 cut(s) 236, 345, 589
BstNSI RCATGY 1 cut(s) 675
BstSCI CCNGG 3 cut(s) 234, 343, 587
BstUI CGCG 1 cut(s) 692
BstV1I GCAGC 3 cut(s) 89, 182, 687
BstV2I GAAGAC 1 cut(s) 471
BstX2I RGATCY 1 cut(s) 628
BstYI RGATCY 1 cut(s) 628
BsuRI GGCC 1 cut(s) 665
BtgZI GCGATG 1 cut(s) 884
BtsCI GGATG 2 cut(s) 221, 448
BtsIMutI CAGTG 1 cut(s) 802
BveI ACCTGC 1 cut(s) 164
Cac8I GCNNGC 3 cut(s) 153, 290, 682
CfoI GCGC 2 cut(s) 362, 680
Cfr13I GGNCC 1 cut(s) 410
CseI GACGC 2 cut(s) 213, 390
Csp6I GTAC 1 cut(s) 621
CspCI CAANNNNNGTGG 2 cut(s) 474, 509
CviAII CATG 3 cut(s) 197, 530, 672
CviQI GTAC 1 cut(s) 621
DdeI CTNAG 6 cut(s) 12, 261, 504, 540, 770, 853
DpnI GATC 3 cut(s) 159, 630, 780
DpnII GATC 3 cut(s) 157, 628, 778
EaeI YGGCCR 1 cut(s) 663
Ecl136II GAGCTC 1 cut(s) 508
Eco24I GRGCYC 1 cut(s) 510
Eco47I GGWCC 1 cut(s) 410
Eco53kI GAGCTC 1 cut(s) 508
EcoICRI GAGCTC 1 cut(s) 508
EcoRII CCWGG 3 cut(s) 234, 343, 587
EcoT38I GRGCYC 1 cut(s) 510
FaeI CATG 3 cut(s) 200, 533, 675
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FatI CATG 3 cut(s) 196, 529, 671
FbaI TGATCA 1 cut(s) 778
FblI GTMKAC 1 cut(s) 735
Fnu4HI GCNGC 4 cut(s) 103, 171, 676, 934
FokI GGATG 2 cut(s) 228, 455
FriOI GRGCYC 1 cut(s) 510
Fsp4HI GCNGC 4 cut(s) 103, 171, 676, 934
FspBI CTAG 2 cut(s) 578, 807
GlaI GCGC 2 cut(s) 361, 679
GluI GCNGC 4 cut(s) 103, 171, 676, 934
GsaI CCCAGC 1 cut(s) 278
GsuI CTGGAG 1 cut(s) 783
HaeIII GGCC 1 cut(s) 665
HapII CCGG 1 cut(s) 413
HgaI GACGC 2 cut(s) 213, 390
HhaI GCGC 2 cut(s) 362, 680
Hin1II CATG 3 cut(s) 200, 533, 675
Hin6I GCGC 2 cut(s) 360, 678
HinP1I GCGC 2 cut(s) 360, 678
HinfI GANTC 4 cut(s) 239, 269, 641, 765
HpaII CCGG 1 cut(s) 413
HphI GGTGA 2 cut(s) 130, 886
Hpy166II GTNNAC 2 cut(s) 736, 820
Hpy188I TCNGA 4 cut(s) 128, 185, 505, 778
Hpy188III TCNNGA 1 cut(s) 769
Hpy8I GTNNAC 2 cut(s) 736, 820
HpyAV CCTTC 5 cut(s) 259, 712, 751, 841, 896
HpyCH4III ACNGT 3 cut(s) 136, 625, 824
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 294, 398, 681
HpyF3I CTNAG 6 cut(s) 12, 261, 504, 540, 770, 853
Hsp92II CATG 3 cut(s) 200, 533, 675
HspAI GCGC 2 cut(s) 360, 678
Ksp22I TGATCA 1 cut(s) 778
Kzo9I GATC 3 cut(s) 157, 628, 778
LmnI GCTCC 2 cut(s) 599, 694
Lsp1109I GCAGC 3 cut(s) 89, 182, 687
LweI GCATC 3 cut(s) 211, 304, 697
MaeI CTAG 2 cut(s) 578, 807
MaeIII GTNAC 1 cut(s) 136
MalI GATC 3 cut(s) 159, 630, 780
MboI GATC 3 cut(s) 157, 628, 778
MboII GAAGA 4 cut(s) 344, 447, 476, 841
MflI RGATCY 1 cut(s) 628
MhlI GDGCHC 4 cut(s) 510, 571, 596, 887
MlsI TGGCCA 1 cut(s) 665
MluCI AATT 3 cut(s) 228, 280, 634
MluNI TGGCCA 1 cut(s) 665
MlyI GAGTC 2 cut(s) 263, 774
MmeI TCCRAC 1 cut(s) 185
MnlI CCTC 7 cut(s) 205, 295, 316, 492, 511, 607, 899
Mox20I TGGCCA 1 cut(s) 665
MscI TGGCCA 1 cut(s) 665
MslI CAYNNNNRTG 2 cut(s) 195, 515
Msp20I TGGCCA 1 cut(s) 665
MspI CCGG 1 cut(s) 413
MspR9I CCNGG 3 cut(s) 236, 345, 589
Mva1269I GAATGC 2 cut(s) 192, 502
MvaI CCWGG 3 cut(s) 236, 345, 589
MvnI CGCG 1 cut(s) 692
MwoI GCNNNNNNNGC 4 cut(s) 152, 294, 398, 681
NdeII GATC 3 cut(s) 157, 628, 778
NlaIII CATG 3 cut(s) 200, 533, 675
NlaIV GGNNCC 2 cut(s) 411, 630
NmuCI GTSAC 1 cut(s) 136
NspI RCATGY 1 cut(s) 675
NspV TTCGAA 1 cut(s) 908
PctI GAATGC 2 cut(s) 192, 502
PfeI GAWTC 2 cut(s) 239, 641
PfoI TCCNGGA 1 cut(s) 234
PkrI GCNGC 4 cut(s) 104, 172, 677, 935
PleI GAGTC 2 cut(s) 263, 773
PpsI GAGTC 2 cut(s) 263, 773
Psp124BI GAGCTC 1 cut(s) 510
Psp6I CCWGG 3 cut(s) 234, 343, 587
PspFI CCCAGC 1 cut(s) 274
PspGI CCWGG 3 cut(s) 234, 343, 587
PspN4I GGNNCC 2 cut(s) 411, 630
PspPI GGNCC 1 cut(s) 410
PsuI RGATCY 1 cut(s) 628
RsaI GTAC 1 cut(s) 622
RsaNI GTAC 1 cut(s) 621
RseI CAYNNNNRTG 2 cut(s) 195, 515
SacI GAGCTC 1 cut(s) 510
SatI GCNGC 4 cut(s) 103, 171, 676, 934
Sau3AI GATC 3 cut(s) 157, 628, 778
Sau96I GGNCC 1 cut(s) 410
SchI GAGTC 2 cut(s) 263, 774
ScrFI CCNGG 3 cut(s) 236, 345, 589
SduI GDGCHC 4 cut(s) 510, 571, 596, 887
SfaNI GCATC 3 cut(s) 211, 304, 697
SfuI TTCGAA 1 cut(s) 908
SinI GGWCC 1 cut(s) 410
SmiMI CAYNNNNRTG 2 cut(s) 195, 515
Sse9I AATT 3 cut(s) 228, 280, 634
SsiI CCGC 2 cut(s) 692, 934
SspMI CTAG 2 cut(s) 578, 807
SstI GAGCTC 1 cut(s) 510
StyD4I CCNGG 3 cut(s) 234, 343, 587
TaaI ACNGT 3 cut(s) 136, 625, 824
TaqI TCGA 4 cut(s) 64, 705, 891, 908
TasI AATT 3 cut(s) 228, 280, 634
TauI GCSGC 1 cut(s) 936
TfiI GAWTC 2 cut(s) 239, 641
TscAI CASTG 1 cut(s) 802
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 3 cut(s) 102, 170, 675
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 1 cut(s) 196
TspGWI ACGGA 1 cut(s) 759
TspRI CASTG 1 cut(s) 802
VpaK11BI GGWCC 1 cut(s) 410
XapI RAATTY 2 cut(s) 280, 634
XceI RCATGY 1 cut(s) 675
XmiI GTMKAC 1 cut(s) 735
XspI CTAG 2 cut(s) 578, 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.