Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0017140

Physical Location & Seq

Genomic Coordinates Reverse (-)

31399050 .. 31399502

Gene Structure 453 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1228400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 453 bp

ATGGCCAACCTCCTCAAGCTCACCACCATTTTCTTCCTCATGTTACATTCACTCCTAGCTCTGCATTTTCAAATGGCCTTCTCATCGTCGGAGCTCGAAGACAATGACGAAGTGTACGTAATCGACTCCCCGAATCCCAACTTCAGATCAAGAAGCAGGTTCTTGGCCAGCATCATCAAAAAAGGTGCACAATGTAACCCAATCAAGAACATTTGCAATGGGATTTCTGCAAACAAAGGGACCGGCATTCTCTATTGCTGCAAGAAACATTGCCGAAATGTTCTTAGCGATAAGAACAATTGTGGAAAATGTGGGAATAAATGCAAGTTTGGAATGCGTTGTTGCAATGGAACTTGCATGAATACTACTTCGGATGCCAACAACTGTGGCAAGTGTGGTAAAAAATGCAAGTATGGGGTTAAATGTGAGTATGGATACTGTGGGTATGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000302 GO:0001101 GO:0003006 GO:0005575 GO:0005576 GO:0005615 GO:0006950 GO:0006952 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009791 GO:0009863 GO:0009867 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010154 GO:0010193 GO:0010337 GO:0010565 GO:0010817 GO:0010941 GO:0010942 GO:0014070 GO:0019216 GO:0019217 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031326 GO:0032350 GO:0032501 GO:0032502 GO:0032870 GO:0035690 GO:0042221 GO:0042304 GO:0042493 GO:0042742 GO:0043207 GO:0044421 GO:0046677 GO:0046885 GO:0046890 GO:0048316 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0062012 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0080090 GO:0080140 GO:0080141 GO:0080142 GO:0098542 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Stig1

Protein Analysis

151

Amino Acids

16.58

Weight (kDa)

9.07

Isoelectric Point (pI)

22.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stig1	PF04885	63 - 150	8.8e-24	Stigma-specific STIG1-like protein family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0017140)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53130
fragaria_vesca	FvH4_3g19820
malus_domestica	MD03G1228400.v1.1
prunus_persica	Prupe.4G180300_v2.0.a1 Prupe.4G180400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0033251
rosa_laevigata	RLG00000033460
rosa_multiflora	Rmu_ssc0000299.1_g000017
rosa_roxburghii	Rroxscaffold_1G00046740
rosa_rugosa	Rorug05G0138100
rosa_samantha	Rh5AG229900 Rh5CG258800
rosa_wichuraiana	Rw5G021020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	147
AcoI	YGGCCR	2 cut(s)	3, 165
AcuI	CTGAAG	1 cut(s)	127
AfaI	GTAC	1 cut(s)	116
AgsI	TTSAA	1 cut(s)	71
AluBI	AGCT	3 cut(s)	19, 59, 94
AluI	AGCT	3 cut(s)	19, 59, 94
Alw21I	GWGCWC	2 cut(s)	96, 190
Alw44I	GTGCAC	1 cut(s)	186
AoxI	GGCC	3 cut(s)	3, 75, 165
ApaLI	GTGCAC	1 cut(s)	186
ApeKI	GCWGC	1 cut(s)	258
AspS9I	GGNCC	1 cut(s)	240
AsuHPI	GGTGA	1 cut(s)	13
AvaII	GGWCC	1 cut(s)	240
BaeGI	GKGCMC	1 cut(s)	190
BalI	TGGCCA	2 cut(s)	5, 167
BanII	GRGCYC	1 cut(s)	96
BbsI	GAAGAC	1 cut(s)	105
Bbv12I	GWGCWC	2 cut(s)	96, 190
BbvI	GCAGC	1 cut(s)	245
BciVI	GTATCC	1 cut(s)	428
BfaI	CTAG	1 cut(s)	56
BfuAI	ACCTGC	1 cut(s)	147
BfuI	GTATCC	1 cut(s)	428
BisI	GCNGC	1 cut(s)	259
BlsI	GCNGC	1 cut(s)	260
Bme18I	GGWCC	1 cut(s)	240
BmgT120I	GGNCC	1 cut(s)	240
BmiI	GGNNCC	1 cut(s)	241
BmsI	GCATC	2 cut(s)	180, 364
BpiI	GAAGAC	1 cut(s)	105
BsaAI	YACGTR	1 cut(s)	118
BsaXI	ACNNNNNCTCC	2 cut(s)	36, 66
Bse118I	RCCGGY	1 cut(s)	242
Bse3DI	GCAATG	3 cut(s)	223, 268, 352
BseGI	GGATG	1 cut(s)	379
BseMI	GCAATG	3 cut(s)	223, 268, 352
BseSI	GKGCMC	1 cut(s)	190
BseXI	GCAGC	1 cut(s)	245
BshFI	GGCC	3 cut(s)	5, 77, 167
BsiHKAI	GWGCWC	2 cut(s)	96, 190
BsiSI	CCGG	1 cut(s)	243
BslFI	GGGAC	1 cut(s)	253
BsmFI	GGGAC	1 cut(s)	253
BsmI	GAATGC	2 cut(s)	246, 339
BsnI	GGCC	3 cut(s)	5, 77, 167
Bsp1286I	GDGCHC	2 cut(s)	96, 190
Bsp143I	GATC	1 cut(s)	146
BspANI	GGCC	3 cut(s)	5, 77, 167
BspLI	GGNNCC	1 cut(s)	241
BspMI	ACCTGC	1 cut(s)	147
BsrDI	GCAATG	3 cut(s)	223, 268, 352
BsrFI	RCCGGY	1 cut(s)	242
BssAI	RCCGGY	1 cut(s)	242
BssMI	GATC	1 cut(s)	146
Bst4CI	ACNGT	2 cut(s)	386, 440
BstBAI	YACGTR	1 cut(s)	118
BstC8I	GCNNGC	1 cut(s)	169
BstDEI	CTNAG	2 cut(s)	284, 450
BstF5I	GGATG	1 cut(s)	379
BstKTI	GATC	1 cut(s)	149
BstMBI	GATC	1 cut(s)	146
BstSLI	GKGCMC	1 cut(s)	190
BstSNI	TACGTA	1 cut(s)	118
BstV1I	GCAGC	1 cut(s)	245
BstV2I	GAAGAC	1 cut(s)	105
BsuI	GTATCC	1 cut(s)	428
BsuRI	GGCC	3 cut(s)	5, 77, 167
BtsCI	GGATG	1 cut(s)	379
BveI	ACCTGC	1 cut(s)	147
Cac8I	GCNNGC	1 cut(s)	169
Cfr10I	RCCGGY	1 cut(s)	242
Cfr13I	GGNCC	1 cut(s)	240
Csp6I	GTAC	1 cut(s)	115
CspCI	CAANNNNNGTGG	2 cut(s)	367, 402
CviAII	CATG	2 cut(s)	40, 358
CviJI	RGCY	6 cut(s)	5, 19, 59, 77, 94, 167
CviKI_1	RGCY	6 cut(s)	5, 19, 59, 77, 94, 167
CviQI	GTAC	1 cut(s)	115
DdeI	CTNAG	2 cut(s)	284, 450
DpnI	GATC	1 cut(s)	148
DpnII	GATC	1 cut(s)	146
EaeI	YGGCCR	2 cut(s)	3, 165
Ecl136II	GAGCTC	1 cut(s)	94
Eco105I	TACGTA	1 cut(s)	118
Eco24I	GRGCYC	1 cut(s)	96
Eco47I	GGWCC	1 cut(s)	240
Eco53kI	GAGCTC	1 cut(s)	94
Eco57I	CTGAAG	1 cut(s)	127
EcoICRI	GAGCTC	1 cut(s)	94
EcoT38I	GRGCYC	1 cut(s)	96
FaeI	CATG	2 cut(s)	43, 361
FaiI	YATR	5 cut(s)	41, 359, 414, 432, 447
FaqI	GGGAC	1 cut(s)	253
FatI	CATG	2 cut(s)	39, 357
Fnu4HI	GCNGC	1 cut(s)	259
FokI	GGATG	1 cut(s)	386
FriOI	GRGCYC	1 cut(s)	96
Fsp4HI	GCNGC	1 cut(s)	259
FspBI	CTAG	1 cut(s)	56
GluI	GCNGC	1 cut(s)	259
HaeIII	GGCC	3 cut(s)	5, 77, 167
HapII	CCGG	1 cut(s)	243
Hin1II	CATG	2 cut(s)	43, 361
HinfI	GANTC	2 cut(s)	125, 133
HpaII	CCGG	1 cut(s)	243
HphI	GGTGA	1 cut(s)	13
Hpy166II	GTNNAC	2 cut(s)	115, 188
Hpy188I	TCNGA	3 cut(s)	91, 146, 373
Hpy188III	TCNNGA	2 cut(s)	150, 205
Hpy8I	GTNNAC	2 cut(s)	115, 188
Hpy99I	CGWCG	1 cut(s)	91
HpyAV	CCTTC	1 cut(s)	88
HpyCH4III	ACNGT	2 cut(s)	386, 440
HpyCH4IV	ACGT	1 cut(s)	117
HpyCH4V	TGCA	9 cut(s)	64, 188, 216, 230, 261, 324, 345, 357, 408
HpyF3I	CTNAG	2 cut(s)	284, 450
HpySE526I	ACGT	1 cut(s)	117
Hsp92II	CATG	2 cut(s)	43, 361
Kzo9I	GATC	1 cut(s)	146
LmnI	GCTCC	1 cut(s)	91
LpnPI	CCDG	3 cut(s)	142, 181, 256
Lsp1109I	GCAGC	1 cut(s)	245
LweI	GCATC	2 cut(s)	180, 364
MaeI	CTAG	1 cut(s)	56
MaeII	ACGT	1 cut(s)	117
MaeIII	GTNAC	2 cut(s)	42, 194
MalI	GATC	1 cut(s)	148
MboI	GATC	1 cut(s)	146
MboII	GAAGA	2 cut(s)	25, 110
MfeI	CAATTG	1 cut(s)	298
MhlI	GDGCHC	2 cut(s)	96, 190
MlsI	TGGCCA	2 cut(s)	5, 167
MluCI	AATT	1 cut(s)	298
MluNI	TGGCCA	2 cut(s)	5, 167
MlyI	GAGTC	1 cut(s)	119
MmeI	TCCRAC	1 cut(s)	69
MnlI	CCTC	3 cut(s)	20, 23, 47
Mox20I	TGGCCA	2 cut(s)	5, 167
MscI	TGGCCA	2 cut(s)	5, 167
MseI	TTAA	1 cut(s)	420
Msp20I	TGGCCA	2 cut(s)	5, 167
MspI	CCGG	1 cut(s)	243
MunI	CAATTG	1 cut(s)	298
Mva1269I	GAATGC	2 cut(s)	246, 339
NdeII	GATC	1 cut(s)	146
NlaIII	CATG	2 cut(s)	43, 361
NlaIV	GGNNCC	1 cut(s)	241
PcsI	WCGNNNNNNNCGW	1 cut(s)	114
PctI	GAATGC	2 cut(s)	246, 339
PfeI	GAWTC	1 cut(s)	133
PkrI	GCNGC	1 cut(s)	260
PleI	GAGTC	1 cut(s)	119
PpsI	GAGTC	1 cut(s)	119
Ppu21I	YACGTR	1 cut(s)	118
Psp124BI	GAGCTC	1 cut(s)	96
PspN4I	GGNNCC	1 cut(s)	241
PspPI	GGNCC	1 cut(s)	240
RsaI	GTAC	1 cut(s)	116
RsaNI	GTAC	1 cut(s)	115
SacI	GAGCTC	1 cut(s)	96
SaqAI	TTAA	1 cut(s)	420
SatI	GCNGC	1 cut(s)	259
Sau3AI	GATC	1 cut(s)	146
Sau96I	GGNCC	1 cut(s)	240
SchI	GAGTC	1 cut(s)	119
SduI	GDGCHC	2 cut(s)	96, 190
SetI	ASST	7 cut(s)	12, 21, 61, 96, 120, 161, 187
SfaNI	GCATC	2 cut(s)	180, 364
SinI	GGWCC	1 cut(s)	240
SmlI	CTYRAG	1 cut(s)	14
SmoI	CTYRAG	1 cut(s)	14
SnaBI	TACGTA	1 cut(s)	118
Sse9I	AATT	1 cut(s)	298
SspMI	CTAG	1 cut(s)	56
SstI	GAGCTC	1 cut(s)	96
TaaI	ACNGT	2 cut(s)	386, 440
TaiI	ACGT	1 cut(s)	120
TaqI	TCGA	2 cut(s)	96, 123
TasI	AATT	1 cut(s)	298
TfiI	GAWTC	1 cut(s)	133
Tru1I	TTAA	1 cut(s)	420
Tru9I	TTAA	1 cut(s)	420
TseI	GCWGC	1 cut(s)	258
TspDTI	ATGAA	1 cut(s)	374
VneI	GTGCAC	1 cut(s)	186
VpaK11BI	GGWCC	1 cut(s)	240
XspI	CTAG	1 cut(s)	56

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.