Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0017140

Physical Location & Seq

Genomic Coordinates Forward (+)

27632132 .. 27638098

Gene Structure 5967 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG229900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 546 bp

ATGTTACGCCATGCGACTGTCCTTTGTATTCGATCTCCAAGTCTTCCGGAACGCTACCCAGAATCGAAGACAGAATTTCTTTCTTTCATCGCAACTTCACAATACCAAATGGCCTTCTCGTCTCAGCTCGAGGACGAAGACGACGAGGAGTACGTGCTCGATGCTCCGAATCCGAACTTCAGATCCAGAAGCAGGTTCTTGGCCAGCATTATCAAGAAAGGAGCGCATTGCGACCCCGTCAAGTACAACATCTGCAATGGGATTTCGGCAAACAATGGAACGAGCATTCTGAACTGCTGTAAGACTCATTGTCGTAACATTCTCGGAGACCGGAATAACTGCGGGAAGTGCGGGCGCAAGTGCAATCAGGGACAGCTTTGCTGCAATGGAAGTTGTACGTATGTTGCTTACAACGCTGATCACTGTGGCAAGTGTGCTAGGAAGTGCTCGGCTGGGGTTAAATGTGAGTCTGGATACTGTGGGTTCGAAAGCAGATCAGGCAATTCGGAGCTTGATGATGATGAATTAGGAGCAGCCATGGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000302 GO:0001101 GO:0003006 GO:0005575 GO:0005576 GO:0005615 GO:0006950 GO:0006952 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009791 GO:0009863 GO:0009867 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010154 GO:0010193 GO:0010337 GO:0010565 GO:0010817 GO:0010941 GO:0010942 GO:0014070 GO:0019216 GO:0019217 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031326 GO:0032350 GO:0032501 GO:0032502 GO:0032870 GO:0035690 GO:0042221 GO:0042304 GO:0042493 GO:0042742 GO:0043207 GO:0044421 GO:0046677 GO:0046885 GO:0046890 GO:0048316 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0062012 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0080090 GO:0080140 GO:0080141 GO:0080142 GO:0098542 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Stig1

Protein Analysis

181

Amino Acids

19.9

Weight (kDa)

6.41

Isoelectric Point (pI)

48.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stig1	PF04885	76 - 162	2.2e-19	Stigma-specific STIG1-like protein family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017140)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53130
fragaria_vesca	FvH4_3g19820
malus_domestica	MD03G1228400.v1.1
prunus_persica	Prupe.4G180300_v2.0.a1 Prupe.4G180400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0033251
rosa_laevigata	RLG00000033460
rosa_multiflora	Rmu_ssc0000299.1_g000017
rosa_roxburghii	Rroxscaffold_1G00046740
rosa_rugosa	Rorug05G0138100
rosa_samantha	Rh5AG229900 Rh5CG258800
rosa_wichuraiana	Rw5G021020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	183
AccIII	TCCGGA	1 cut(s)	46
AciI	CCGC	2 cut(s)	342, 351
AclWI	GGATC	1 cut(s)	177
AcoI	YGGCCR	1 cut(s)	201
AcsI	RAATTY	1 cut(s)	74
AcuI	CTGAAG	1 cut(s)	163
AfaI	GTAC	3 cut(s)	152, 245, 397
AfiI	CCNNNNNNNGG	1 cut(s)	192
AhdI	GACNNNNNGTC	1 cut(s)	309
AloI	GAACNNNNNNTCC	2 cut(s)	167, 199
AluBI	AGCT	3 cut(s)	127, 376, 511
AluI	AGCT	3 cut(s)	127, 376, 511
Alw21I	GWGCWC	2 cut(s)	159, 449
Alw26I	GTCTC	2 cut(s)	126, 321
AlwI	GGATC	1 cut(s)	177
Ama87I	CYCGRG	1 cut(s)	128
Aor13HI	TCCGGA	1 cut(s)	46
AoxI	GGCC	2 cut(s)	111, 201
ApeKI	GCWGC	2 cut(s)	381, 533
ApoI	RAATTY	1 cut(s)	74
AspLEI	GCGC	2 cut(s)	226, 357
AsuII	TTCGAA	1 cut(s)	486
AvaI	CYCGRG	1 cut(s)	128
BalI	TGGCCA	1 cut(s)	203
BbsI	GAAGAC	3 cut(s)	35, 74, 144
Bbv12I	GWGCWC	2 cut(s)	159, 449
BbvI	GCAGC	1 cut(s)	368
BciVI	GTATCC	1 cut(s)	467
BclI	TGATCA	1 cut(s)	418
BcoDI	GTCTC	2 cut(s)	126, 321
BfaI	CTAG	1 cut(s)	438
BfuAI	ACCTGC	1 cut(s)	183
BfuI	GTATCC	1 cut(s)	467
BisI	GCNGC	2 cut(s)	382, 534
BlsI	GCNGC	2 cut(s)	383, 535
BmeRI	GACNNNNNGTC	1 cut(s)	309
BmeT110I	CYCGRG	1 cut(s)	128
BmsI	GCATC	1 cut(s)	151
BpiI	GAAGAC	3 cut(s)	35, 74, 144
Bpu14I	TTCGAA	1 cut(s)	486
BsaAI	YACGTR	2 cut(s)	154, 399
BsaI	GGTCTC	1 cut(s)	321
BsaJI	CCNNGG	1 cut(s)	537
BsaWI	WCCGGW	2 cut(s)	46, 330
Bsc4I	CCNNNNNNNGG	1 cut(s)	192
Bse3DI	GCAATG	3 cut(s)	226, 262, 391
BseAI	TCCGGA	1 cut(s)	46
BseDI	CCNNGG	1 cut(s)	537
BseLI	CCNNNNNNNGG	1 cut(s)	192
BseMI	GCAATG	3 cut(s)	226, 262, 391
BseMII	CTCAG	1 cut(s)	137
BseRI	GAGGAG	1 cut(s)	161
BseXI	GCAGC	1 cut(s)	368
BseYI	CCCAGC	1 cut(s)	452
BshFI	GGCC	2 cut(s)	113, 203
BsiHKAI	GWGCWC	2 cut(s)	159, 449
BsiHKCI	CYCGRG	1 cut(s)	128
BsiSI	CCGG	2 cut(s)	47, 331
BslFI	GGGAC	1 cut(s)	384
BslI	CCNNNNNNNGG	1 cut(s)	192
BsmAI	GTCTC	2 cut(s)	126, 321
BsmBI	CGTCTC	1 cut(s)	126
BsmFI	GGGAC	1 cut(s)	384
BsmI	GAATGC	1 cut(s)	285
BsnI	GGCC	2 cut(s)	113, 203
Bso31I	GGTCTC	1 cut(s)	321
BsoBI	CYCGRG	1 cut(s)	128
Bsp119I	TTCGAA	1 cut(s)	486
Bsp1286I	GDGCHC	2 cut(s)	159, 449
Bsp13I	TCCGGA	1 cut(s)	46
Bsp143I	GATC	4 cut(s)	32, 182, 418, 494
Bsp19I	CCATGG	1 cut(s)	537
BspACI	CCGC	2 cut(s)	342, 351
BspANI	GGCC	2 cut(s)	113, 203
BspCNI	CTCAG	1 cut(s)	136
BspEI	TCCGGA	1 cut(s)	46
BspMI	ACCTGC	1 cut(s)	183
BspPI	GGATC	1 cut(s)	177
BspT104I	TTCGAA	1 cut(s)	486
BspTNI	GGTCTC	1 cut(s)	321
BsrDI	GCAATG	3 cut(s)	226, 262, 391
BssECI	CCNNGG	1 cut(s)	537
BssMI	GATC	4 cut(s)	32, 182, 418, 494
BssT1I	CCWWGG	1 cut(s)	537
Bst4CI	ACNGT	3 cut(s)	19, 425, 479
BstBAI	YACGTR	2 cut(s)	154, 399
BstBI	TTCGAA	1 cut(s)	486
BstC8I	GCNNGC	2 cut(s)	205, 353
BstDEI	CTNAG	1 cut(s)	123
BstDSI	CCRYGG	1 cut(s)	537
BstHHI	GCGC	2 cut(s)	226, 357
BstKTI	GATC	4 cut(s)	35, 185, 421, 497
BstMAI	GTCTC	2 cut(s)	126, 321
BstMBI	GATC	4 cut(s)	32, 182, 418, 494
BstMWI	GCNNNNNNNGC	3 cut(s)	348, 413, 498
BstSNI	TACGTA	1 cut(s)	399
BstV1I	GCAGC	1 cut(s)	368
BstV2I	GAAGAC	3 cut(s)	35, 74, 144
BstX2I	RGATCY	1 cut(s)	182
BstYI	RGATCY	1 cut(s)	182
BsuI	GTATCC	1 cut(s)	467
BsuRI	GGCC	2 cut(s)	113, 203
BtgI	CCRYGG	1 cut(s)	537
BtgZI	GCGATG	1 cut(s)	73
BtsIMutI	CAGTG	1 cut(s)	421
BveI	ACCTGC	1 cut(s)	183
Cac8I	GCNNGC	2 cut(s)	205, 353
CfoI	GCGC	2 cut(s)	226, 357
Csp6I	GTAC	3 cut(s)	151, 244, 396
CviAII	CATG	2 cut(s)	11, 538
CviJI	RGCY	7 cut(s)	113, 127, 203, 376, 452, 511, 536
CviKI_1	RGCY	7 cut(s)	113, 127, 203, 376, 452, 511, 536
CviQI	GTAC	3 cut(s)	151, 244, 396
DdeI	CTNAG	1 cut(s)	123
DpnI	GATC	4 cut(s)	34, 184, 420, 496
DpnII	GATC	4 cut(s)	32, 182, 418, 494
DriI	GACNNNNNGTC	1 cut(s)	309
EaeI	YGGCCR	1 cut(s)	201
Eam1105I	GACNNNNNGTC	1 cut(s)	309
Eco105I	TACGTA	1 cut(s)	399
Eco130I	CCWWGG	1 cut(s)	537
Eco31I	GGTCTC	1 cut(s)	321
Eco57I	CTGAAG	1 cut(s)	163
Eco88I	CYCGRG	1 cut(s)	128
EcoT14I	CCWWGG	1 cut(s)	537
ErhI	CCWWGG	1 cut(s)	537
Esp3I	CGTCTC	1 cut(s)	126
FaeI	CATG	2 cut(s)	14, 541
FaiI	YATR	3 cut(s)	12, 402, 539
FaqI	GGGAC	1 cut(s)	384
FatI	CATG	2 cut(s)	10, 537
FauI	CCCGC	2 cut(s)	335, 344
FbaI	TGATCA	1 cut(s)	418
Fnu4HI	GCNGC	2 cut(s)	382, 534
Fsp4HI	GCNGC	2 cut(s)	382, 534
FspBI	CTAG	1 cut(s)	438
GlaI	GCGC	2 cut(s)	225, 356
GluI	GCNGC	2 cut(s)	382, 534
GsaI	CCCAGC	1 cut(s)	456
HaeIII	GGCC	2 cut(s)	113, 203
HapII	CCGG	2 cut(s)	47, 331
HhaI	GCGC	2 cut(s)	226, 357
Hin1II	CATG	2 cut(s)	14, 541
Hin6I	GCGC	2 cut(s)	224, 355
HinP1I	GCGC	2 cut(s)	224, 355
HinfI	GANTC	4 cut(s)	62, 169, 304, 467
HpaII	CCGG	2 cut(s)	47, 331
Hpy188I	TCNGA	6 cut(s)	168, 174, 182, 291, 326, 508
Hpy188III	TCNNGA	4 cut(s)	47, 186, 214, 471
Hpy99I	CGWCG	1 cut(s)	146
HpyAV	CCTTC	1 cut(s)	124
HpyCH4III	ACNGT	3 cut(s)	19, 425, 479
HpyCH4IV	ACGT	2 cut(s)	153, 398
HpyCH4V	TGCA	3 cut(s)	255, 363, 384
HpyF10VI	GCNNNNNNNGC	3 cut(s)	348, 413, 498
HpyF3I	CTNAG	1 cut(s)	123
HpySE526I	ACGT	2 cut(s)	153, 398
Hsp92II	CATG	2 cut(s)	14, 541
HspAI	GCGC	2 cut(s)	224, 355
Kpn2I	TCCGGA	1 cut(s)	46
Ksp22I	TGATCA	1 cut(s)	418
Kzo9I	GATC	4 cut(s)	32, 182, 418, 494
LmnI	GCTCC	4 cut(s)	169, 221, 508, 530
Lsp1109I	GCAGC	1 cut(s)	368
LweI	GCATC	1 cut(s)	151
MaeI	CTAG	1 cut(s)	438
MaeII	ACGT	2 cut(s)	153, 398
MaeIII	GTNAC	2 cut(s)	3, 314
MalI	GATC	4 cut(s)	34, 184, 420, 496
MboI	GATC	4 cut(s)	32, 182, 418, 494
MboII	GAAGA	3 cut(s)	35, 79, 149
MflI	RGATCY	1 cut(s)	182
MhlI	GDGCHC	2 cut(s)	159, 449
MlsI	TGGCCA	1 cut(s)	203
MluCI	AATT	3 cut(s)	74, 502, 524
MluNI	TGGCCA	1 cut(s)	203
MlyI	GAGTC	2 cut(s)	298, 476
MnlI	CCTC	2 cut(s)	124, 139
Mox20I	TGGCCA	1 cut(s)	203
MroI	TCCGGA	1 cut(s)	46
MscI	TGGCCA	1 cut(s)	203
MseI	TTAA	1 cut(s)	459
Msp20I	TGGCCA	1 cut(s)	203
MspI	CCGG	2 cut(s)	47, 331
Mva1269I	GAATGC	1 cut(s)	285
MwoI	GCNNNNNNNGC	3 cut(s)	348, 413, 498
NcoI	CCATGG	1 cut(s)	537
NdeII	GATC	4 cut(s)	32, 182, 418, 494
NlaIII	CATG	2 cut(s)	14, 541
NmeAIII	GCCGAG	1 cut(s)	428
NspV	TTCGAA	1 cut(s)	486
PaeR7I	CTCGAG	1 cut(s)	128
PcsI	WCGNNNNNNNCGW	2 cut(s)	141, 150
PctI	GAATGC	1 cut(s)	285
PfeI	GAWTC	2 cut(s)	62, 169
PflFI	GACNNNGTC	1 cut(s)	236
PkrI	GCNGC	2 cut(s)	383, 535
PleI	GAGTC	2 cut(s)	298, 475
PpsI	GAGTC	2 cut(s)	298, 475
Ppu21I	YACGTR	2 cut(s)	154, 399
PspFI	CCCAGC	1 cut(s)	452
PspXI	VCTCGAGB	1 cut(s)	128
PsuI	RGATCY	1 cut(s)	182
PsyI	GACNNNGTC	1 cut(s)	236
RsaI	GTAC	3 cut(s)	152, 245, 397
RsaNI	GTAC	3 cut(s)	151, 244, 396
SaqAI	TTAA	1 cut(s)	459
SatI	GCNGC	2 cut(s)	382, 534
Sau3AI	GATC	4 cut(s)	32, 182, 418, 494
SchI	GAGTC	2 cut(s)	298, 476
SduI	GDGCHC	2 cut(s)	159, 449
SetI	ASST	6 cut(s)	129, 156, 197, 378, 401, 513
SfaNI	GCATC	1 cut(s)	151
Sfr274I	CTCGAG	1 cut(s)	128
SfuI	TTCGAA	1 cut(s)	486
SlaI	CTCGAG	1 cut(s)	128
SmlI	CTYRAG	1 cut(s)	128
SmoI	CTYRAG	1 cut(s)	128
SnaBI	TACGTA	1 cut(s)	399
Sse9I	AATT	3 cut(s)	74, 502, 524
SsiI	CCGC	2 cut(s)	342, 351
SspMI	CTAG	1 cut(s)	438
StyI	CCWWGG	1 cut(s)	537
TaaI	ACNGT	3 cut(s)	19, 425, 479
TaiI	ACGT	2 cut(s)	156, 401
TaqI	TCGA	5 cut(s)	31, 65, 129, 159, 486
TasI	AATT	3 cut(s)	74, 502, 524
TatI	WGTACW	1 cut(s)	243
TfiI	GAWTC	2 cut(s)	62, 169
Tru1I	TTAA	1 cut(s)	459
Tru9I	TTAA	1 cut(s)	459
TscAI	CASTG	1 cut(s)	428
TseI	GCWGC	2 cut(s)	381, 533
TspDTI	ATGAA	2 cut(s)	76, 537
TspRI	CASTG	1 cut(s)	428
Tth111I	GACNNNGTC	1 cut(s)	236
XapI	RAATTY	1 cut(s)	74
XhoI	CTCGAG	1 cut(s)	128
XspI	CTAG	1 cut(s)	438

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.