MD03G1277900.v1.1

Monothiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012965
Physical Location & Seq
Reverse (-)
36009631 .. 36009930
300 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1277900.v1.1.491

Sequence Viewer

Length: 300 bp
ATGGACAAAGTAATGAGGTTGGCATCAGAGAAGGGTGTGGTGATTTTTAGCAAGAGCTCATGTTGCCTGTGTTACGCAGTCAAAATTCTATTCCAAGAAATTGGGGTGAGTCCTGTGGTTCATGAGATTGACCAAGATCCCGAGGGCCGGGAAATGGAGAAGGCTCTTACGAGGATGGGGTGTACCGCGCCAGTGCCCGCTGTGTTCATAGGTGGAAATCTCATTGGATCGACCAACGAAGTCATGTCCCTCCACCTAAAAGGCCAGCTGATCCCAAAGCTGAAGCCTTATCAGCAGTAG

Protein Analysis

100

Amino Acids

10.85

Weight (kDa)

7.67

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 13 - 75 4.2e-13 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012965)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06830 AT2G30540 AT2G47880 AT3G62960
fragaria_vesca FvH4_2g24940
malus_domestica MD03G1277900.v1.1 MD11G1299800.v1.1
prunus_persica Prupe.8G254200_v2.0.a1
pyrus_communis pycom03g22130 pycom11g26350
rosa_chinensis RchiOBHm_Chr6g0292861
rosa_laevigata RLG00000011986
rosa_rugosa Rorug06G0230300
rosa_samantha Rh6AG343600 Rh6BG350800 Rh6CG357800 Rh6DG343700
rosa_wichuraiana Rw6G029970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 188
AciI CCGC 2 cut(s) 186, 198
AclWI GGATC 3 cut(s) 131, 235, 265
AcsI RAATTY 1 cut(s) 84
AfaI GTAC 1 cut(s) 184
AfiI CCNNNNNNNGG 2 cut(s) 147, 154
AluBI AGCT 3 cut(s) 57, 268, 280
AluI AGCT 3 cut(s) 57, 268, 280
Alw21I GWGCWC 1 cut(s) 59
AlwI GGATC 3 cut(s) 131, 235, 265
Ama87I CYCGRG 1 cut(s) 140
AoxI GGCC 2 cut(s) 145, 262
ApoI RAATTY 1 cut(s) 84
AspLEI GCGC 1 cut(s) 190
AspS9I GGNCC 1 cut(s) 145
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 2 cut(s) 52, 118
AvaI CYCGRG 1 cut(s) 140
BaeGI GKGCMC 1 cut(s) 198
BanII GRGCYC 1 cut(s) 59
Bbv12I GWGCWC 1 cut(s) 59
BccI CCATC 1 cut(s) 169
BcnI CCSGG 1 cut(s) 149
Bme1390I CCNGG 1 cut(s) 149
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 145
BmrFI CCNGG 1 cut(s) 149
BmsI GCATC 1 cut(s) 32
BpuMI CCSGG 1 cut(s) 149
BsaJI CCNNGG 1 cut(s) 141
Bsc4I CCNNNNNNNGG 2 cut(s) 147, 154
Bse1I ACTGG 1 cut(s) 191
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 1 cut(s) 180
BseLI CCNNNNNNNGG 2 cut(s) 147, 154
BseNI ACTGG 1 cut(s) 191
BseSI GKGCMC 1 cut(s) 198
Bsh1236I CGCG 1 cut(s) 188
BshFI GGCC 2 cut(s) 147, 264
BsiHKAI GWGCWC 1 cut(s) 59
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 1 cut(s) 148
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 2 cut(s) 147, 154
BsmFI GGGAC 1 cut(s) 232
BsnI GGCC 2 cut(s) 147, 264
BsoBI CYCGRG 1 cut(s) 140
Bsp1286I GDGCHC 2 cut(s) 59, 198
Bsp143I GATC 3 cut(s) 136, 227, 270
BspACI CCGC 2 cut(s) 186, 198
BspANI GGCC 2 cut(s) 147, 264
BspFNI CGCG 1 cut(s) 188
BspHI TCATGA 1 cut(s) 121
BspPI GGATC 3 cut(s) 131, 235, 265
BsrI ACTGG 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 141
BssMI GATC 3 cut(s) 136, 227, 270
BstC8I GCNNGC 2 cut(s) 198, 266
BstF5I GGATG 1 cut(s) 180
BstFNI CGCG 1 cut(s) 188
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 3 cut(s) 139, 230, 273
BstMBI GATC 3 cut(s) 136, 227, 270
BstMWI GCNNNNNNNGC 2 cut(s) 63, 292
BstSCI CCNGG 1 cut(s) 147
BstSLI GKGCMC 1 cut(s) 198
BstUI CGCG 1 cut(s) 188
BstX2I RGATCY 1 cut(s) 136
BstXI CCANNNNNNTGG 1 cut(s) 101
BstYI RGATCY 1 cut(s) 136
BsuRI GGCC 2 cut(s) 147, 264
BtsCI GGATG 1 cut(s) 180
BtsIMutI CAGTG 1 cut(s) 198
Cac8I GCNNGC 2 cut(s) 198, 266
CciI TCATGA 1 cut(s) 121
CfoI GCGC 1 cut(s) 190
Cfr13I GGNCC 1 cut(s) 145
Csp6I GTAC 1 cut(s) 183
CviAII CATG 3 cut(s) 60, 122, 244
CviJI RGCY 7 cut(s) 57, 147, 164, 264, 268, 280, 286
CviKI_1 RGCY 7 cut(s) 57, 147, 164, 264, 268, 280, 286
CviQI GTAC 1 cut(s) 183
DpnI GATC 3 cut(s) 138, 229, 272
DpnII GATC 3 cut(s) 136, 227, 270
Ecl136II GAGCTC 1 cut(s) 57
Eco24I GRGCYC 1 cut(s) 59
Eco53kI GAGCTC 1 cut(s) 57
Eco88I CYCGRG 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 57
EcoT38I GRGCYC 1 cut(s) 59
FaeI CATG 3 cut(s) 63, 125, 247
FaiI YATR 4 cut(s) 61, 123, 209, 245
FaqI GGGAC 1 cut(s) 232
FatI CATG 3 cut(s) 59, 121, 243
FauI CCCGC 1 cut(s) 205
FokI GGATG 1 cut(s) 187
FriOI GRGCYC 1 cut(s) 59
GlaI GCGC 1 cut(s) 189
HaeIII GGCC 2 cut(s) 147, 264
HapII CCGG 1 cut(s) 148
HhaI GCGC 1 cut(s) 190
Hin1II CATG 3 cut(s) 63, 125, 247
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HinfI GANTC 1 cut(s) 109
HpaII CCGG 1 cut(s) 148
HphI GGTGA 2 cut(s) 52, 118
Hpy166II GTNNAC 1 cut(s) 183
Hpy188I TCNGA 1 cut(s) 28
Hpy188III TCNNGA 2 cut(s) 122, 140
Hpy8I GTNNAC 1 cut(s) 183
HpyAV CCTTC 2 cut(s) 25, 154
HpyF10VI GCNNNNNNNGC 2 cut(s) 63, 292
Hsp92II CATG 3 cut(s) 63, 125, 247
HspAI GCGC 1 cut(s) 188
Kzo9I GATC 3 cut(s) 136, 227, 270
LpnPI CCDG 5 cut(s) 80, 126, 161, 204, 278
LweI GCATC 1 cut(s) 32
MaeIII GTNAC 1 cut(s) 71
MalI GATC 3 cut(s) 138, 229, 272
MboI GATC 3 cut(s) 136, 227, 270
MflI RGATCY 1 cut(s) 136
MhlI GDGCHC 2 cut(s) 59, 198
MluCI AATT 2 cut(s) 84, 99
MlyI GAGTC 1 cut(s) 118
MnlI CCTC 4 cut(s) 9, 136, 165, 260
MspA1I CMGCKG 2 cut(s) 200, 268
MspI CCGG 1 cut(s) 148
MspR9I CCNGG 1 cut(s) 149
MvnI CGCG 1 cut(s) 188
MwoI GCNNNNNNNGC 2 cut(s) 63, 292
NciI CCSGG 1 cut(s) 149
NdeII GATC 3 cut(s) 136, 227, 270
NlaIII CATG 3 cut(s) 63, 125, 247
PagI TCATGA 1 cut(s) 121
PleI GAGTC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 117
Psp124BI GAGCTC 1 cut(s) 59
PspPI GGNCC 1 cut(s) 145
PsuI RGATCY 1 cut(s) 136
PvuII CAGCTG 1 cut(s) 268
RsaI GTAC 1 cut(s) 184
RsaNI GTAC 1 cut(s) 183
SacI GAGCTC 1 cut(s) 59
Sau3AI GATC 3 cut(s) 136, 227, 270
Sau96I GGNCC 1 cut(s) 145
SchI GAGTC 1 cut(s) 118
ScrFI CCNGG 1 cut(s) 149
SduI GDGCHC 2 cut(s) 59, 198
SetI ASST 6 cut(s) 20, 59, 214, 258, 270, 282
SfaNI GCATC 1 cut(s) 32
Sse9I AATT 2 cut(s) 84, 99
SsiI CCGC 2 cut(s) 186, 198
SstI GAGCTC 1 cut(s) 59
StyD4I CCNGG 1 cut(s) 147
TaqI TCGA 1 cut(s) 230
TasI AATT 2 cut(s) 84, 99
TscAI CASTG 1 cut(s) 198
TspDTI ATGAA 2 cut(s) 110, 196
TspRI CASTG 1 cut(s) 198
XapI RAATTY 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.